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EA201890032A1 - Термостабильные нуклеазы cas9 - Google Patents

Термостабильные нуклеазы cas9

Info

Publication number
EA201890032A1
EA201890032A1 EA201890032A EA201890032A EA201890032A1 EA 201890032 A1 EA201890032 A1 EA 201890032A1 EA 201890032 A EA201890032 A EA 201890032A EA 201890032 A EA201890032 A EA 201890032A EA 201890032 A1 EA201890032 A1 EA 201890032A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cas9
genetic engineering
cas
nucleases
protein
Prior art date
Application number
EA201890032A
Other languages
English (en)
Other versions
EA038500B1 (ru
Inventor
Джон Ост Ван Дер
Мартинус Йоханнес Арнолдус Дас
Серватиус Вилхелмус Мария Кенген
Виллем Мейндерт Вос Де
Original Assignee
Пюрак Биокем Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Пюрак Биокем Б.В. filed Critical Пюрак Биокем Б.В.
Publication of EA201890032A1 publication Critical patent/EA201890032A1/ru
Publication of EA038500B1 publication Critical patent/EA038500B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)

Abstract

Изобретение относится к области генной инженерии и более конкретно к редактированию нуклеиновых кислот и модификации генома. В изобретении предлагается выделенный белок Cas или его полипептидный фрагмент, имеющий аминокислотную последовательность SEQ ID NO: 1 или последовательность, идентичную ей по меньшей мере на 77%, где белок или полипептид Cas способен расщеплять ДНК при температуре в диапазоне от 50 до 100°C включительно. В изобретении дополнительно предлагаются выделенные молекулы нуклеиновой кислоты, кодирующие указанные нуклеазы Cas9, экспрессионные векторы и клетки-хозяева. Нуклеазы Cas9, раскрытые в настоящем изобретении, обеспечивают новыми инструментами для генной инженерии при повышенных температурах и являются особенно ценными для генетических манипуляций с термофильными организмами; особенно микроорганизмами.
EA201890032A 2015-06-12 2016-06-06 Термостабильные нуклеазы cas9 EA038500B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1510296.5A GB201510296D0 (en) 2015-06-12 2015-06-12 Thermostable CAS9 nucleases
PCT/EP2016/062817 WO2016198361A1 (en) 2015-06-12 2016-06-06 Thermostable cas9 nucleases

Publications (2)

Publication Number Publication Date
EA201890032A1 true EA201890032A1 (ru) 2018-07-31
EA038500B1 EA038500B1 (ru) 2021-09-07

Family

ID=53784593

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201890032A EA038500B1 (ru) 2015-06-12 2016-06-06 Термостабильные нуклеазы cas9

Country Status (11)

Country Link
US (2) US10731142B2 (ru)
EP (1) EP3307883A1 (ru)
JP (1) JP7088528B2 (ru)
KR (1) KR20180019655A (ru)
CN (1) CN107922931B (ru)
AU (1) AU2016274452C1 (ru)
CA (1) CA2989084A1 (ru)
EA (1) EA038500B1 (ru)
GB (1) GB201510296D0 (ru)
PH (1) PH12017502281A1 (ru)
WO (1) WO2016198361A1 (ru)

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WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US20150166982A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
CA2956224A1 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
CN108699116A (zh) 2015-10-23 2018-10-23 哈佛大学的校长及成员们 用于基因编辑的演化的cas9蛋白
AU2017306676B2 (en) 2016-08-03 2024-02-22 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
WO2018109101A1 (en) * 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases
BR112019012155A2 (pt) * 2016-12-14 2019-11-12 Stichting Technische Wetenschappen uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
JP7191388B2 (ja) 2017-03-23 2022-12-19 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸によってプログラム可能なｄｎａ結合蛋白質を含む核酸塩基編集因子
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11692184B2 (en) 2017-05-16 2023-07-04 The Regents Of The University Of California Thermostable RNA-guided endonucleases and methods of use thereof
US11732274B2 (en) 2017-07-28 2023-08-22 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
GB201716590D0 (en) * 2017-10-10 2017-11-22 Univ Wageningen Thermostable cas9 nucleases with reduced off-target activity
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
WO2020081808A1 (en) * 2018-10-18 2020-04-23 Chan Zuckerberg Biohub, Inc. Methods and compositions involving thermostable cas9 protein variants
WO2020089448A1 (en) 2018-11-01 2020-05-07 Keygene N.V. Dual guide rna for crispr/cas genome editing in plants cells
RU2712497C1 (ru) * 2018-11-26 2020-01-29 Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий Средство разрезания ДНК на основе Cas9 белка из биотехнологически значимой бактерии Clostridium cellulolyticum
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GB2616539B (en) * 2019-01-07 2023-12-20 Crisp Hr Therapeutics Inc A non-toxic cas9 enzyme and application thereof
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CN111979257B (zh) * 2019-05-22 2023-10-13 上海凯赛生物技术股份有限公司 一种重组dna及其应用
CN114787347B (zh) * 2019-12-10 2024-07-12 因思科瑞普特公司 新颖的mad核酸酶
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US20240002904A1 (en) 2020-11-24 2024-01-04 Keygene N.V. Targeted enrichment using nanopore selective sequencing

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WO2010054108A2 (en) * 2008-11-06 2010-05-14 University Of Georgia Research Foundation, Inc. Cas6 polypeptides and methods of use
GB2518764C (en) 2012-05-25 2016-08-24 Univ California Methods and compositions for RNA-directed target DNA modification
WO2016106239A1 (en) * 2014-12-23 2016-06-30 The Regents Of The University Of California Methods and compositions for nucleic acid integration
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
BR112019012155A2 (pt) * 2016-12-14 2019-11-12 Stichting Technische Wetenschappen uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína

Also Published As

Publication number Publication date
KR20180019655A (ko) 2018-02-26
BR112017026719A2 (pt) 2018-08-28
US20200385697A1 (en) 2020-12-10
JP7088528B2 (ja) 2022-06-21
WO2016198361A1 (en) 2016-12-15
US20180171314A1 (en) 2018-06-21
PH12017502281A1 (en) 2018-06-11
EP3307883A1 (en) 2018-04-18
AU2016274452C1 (en) 2022-03-17
US11802277B2 (en) 2023-10-31
JP2018522544A (ja) 2018-08-16
AU2016274452B2 (en) 2021-12-02
GB201510296D0 (en) 2015-07-29
CN107922931B (zh) 2022-07-26
BR112017026719A8 (pt) 2022-07-05
AU2016274452A1 (en) 2018-01-25
EA038500B1 (ru) 2021-09-07
CA2989084A1 (en) 2016-12-15
CN107922931A (zh) 2018-04-17
US10731142B2 (en) 2020-08-04

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