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DK3287523T3 - Fremgangsmåde til sekretorisk fremstilling af protein - Google Patents

Fremgangsmåde til sekretorisk fremstilling af protein Download PDF

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Publication number
DK3287523T3
DK3287523T3 DK16783245.0T DK16783245T DK3287523T3 DK 3287523 T3 DK3287523 T3 DK 3287523T3 DK 16783245 T DK16783245 T DK 16783245T DK 3287523 T3 DK3287523 T3 DK 3287523T3
Authority
DK
Denmark
Prior art keywords
protein
procedure
secretory production
secretory
production
Prior art date
Application number
DK16783245.0T
Other languages
English (en)
Inventor
Yoshihiko Matsuda
Yumi Ito
Yukari Kashima
Naoko Yamada
Noriko Tsurui
Hiroshi Itaya
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Application granted granted Critical
Publication of DK3287523T3 publication Critical patent/DK3287523T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/13Protein-histidine kinases (2.7.13)
    • C12Y207/13003Histidine kinase (2.7.13.3)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DK16783245.0T 2015-04-24 2016-04-21 Fremgangsmåde til sekretorisk fremstilling af protein DK3287523T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2015089046 2015-04-24
PCT/JP2016/062675 WO2016171224A1 (ja) 2015-04-24 2016-04-21 タンパク質の分泌生産法

Publications (1)

Publication Number Publication Date
DK3287523T3 true DK3287523T3 (da) 2022-07-25

Family

ID=57143158

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16783245.0T DK3287523T3 (da) 2015-04-24 2016-04-21 Fremgangsmåde til sekretorisk fremstilling af protein

Country Status (8)

Country Link
US (1) US10538798B2 (da)
EP (1) EP3287523B1 (da)
JP (1) JP6741000B2 (da)
KR (1) KR102619848B1 (da)
CN (1) CN107532163A (da)
AU (1) AU2016253217B2 (da)
DK (1) DK3287523T3 (da)
WO (1) WO2016171224A1 (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017347016B2 (en) 2016-10-21 2021-09-23 Ajinomoto Co., Inc. Secretory production method for protein
EP3530747A4 (en) 2016-10-21 2020-07-22 Ajinomoto Co., Inc. SECRETORY PROTEIN PRODUCTION PROCESS
WO2019078095A1 (en) 2017-10-16 2019-04-25 Ajinomoto Co., Inc. PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY
CN108330095B (zh) * 2018-03-01 2020-12-29 江南大学 一种积累n-乙酰神经氨酸的重组谷氨酸棒杆菌及其应用
KR20210002575A (ko) * 2018-04-20 2021-01-08 아지노모토 가부시키가이샤 단백질의 분비 생산법
CN110511953B (zh) * 2018-08-31 2022-09-30 江南大学 一种适用于谷氨酸棒杆菌的重组表达载体、外源蛋白表达系统、应用和木聚糖酶的制备方法
WO2020071538A1 (en) 2018-10-05 2020-04-09 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
AU2019364829A1 (en) * 2018-10-25 2021-06-03 Ajinomoto Co., Inc. Method for secretory production of protein
CN113227124A (zh) 2018-10-31 2021-08-06 味之素株式会社 具有针对抗体的亲和性物质、切割性部分及反应性基团的化合物或其盐
CN111434770B (zh) * 2019-01-11 2022-12-06 华东师范大学 蛋白质谷氨酰胺酶酶原在枯草芽孢杆菌中的表达和自加工
AU2020396647A1 (en) 2019-12-06 2022-07-07 Ajinomoto Co., Inc. Method for producing peptide having physiological activity, and peptide comprising short linker
JPWO2023282315A1 (da) 2021-07-07 2023-01-12
JPWO2023063314A1 (da) * 2021-10-11 2023-04-20
WO2024172163A1 (ja) * 2023-02-16 2024-08-22 味の素株式会社 タンパク質の分泌生産法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS592689A (ja) * 1982-06-04 1984-01-09 Handai Biseibutsubiyou Kenkyukai 強力な遺伝子発現能を有する新規レプリコンの作成法
US4965197A (en) 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
EP0551506A1 (fr) 1991-07-30 1993-07-21 Orsan Systeme d'expression et de secretion de proteines utilisables en particulier chez les corynebacteries
JPH11169182A (ja) 1997-12-10 1999-06-29 Mitsubishi Chemical Corp コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
EP1219713B1 (en) 1999-09-30 2011-11-09 Ajinomoto Co., Inc. Process for producing transglutaminase
ES2335750T3 (es) 2001-03-30 2010-04-05 Ajinomoto Co., Inc. Metodo de secrecion y de produccion de proteinas.
GB0224082D0 (en) * 2002-10-16 2002-11-27 Celltech R&D Ltd Biological products
DK1748077T3 (da) * 2004-04-20 2013-04-08 Ajinomoto Kk Fremgangsmåde til fremstilling af protein
CN100554431C (zh) * 2004-04-20 2009-10-28 味之素株式会社 生产蛋白质的方法
BRPI0512818A (pt) * 2004-07-02 2007-10-23 Metanomics Gmbh processos para a produção do respectivo produto quìmico fino e de um polipeptìdeo e para a identificação de um composto conferindo produção incrementada do respectivo produto quìmico fino, molécula de ácido nucleico isolada, construção de ácido nucleico, vetor, célula hospedeira, polipeptìdeo, anticorpo, tecido de planta, material de propagação, material colhido ou uma planta. métodos para selecionar agonistas e antagonistas da atividade de um polipeptìdeo, para identificar um produto gênico e para produzir uma composição agrìcola, composição e usos de uma molécula de ácido nucleico e de um polipeptìdeo e célula de planta, organela de célula de planta, tecido de planta, planta ou parte da mesma
CA2559760A1 (en) * 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
US7618799B2 (en) * 2007-01-31 2009-11-17 Dow Global Technologies Inc Bacterial leader sequences for increased expression
AU2012329967B2 (en) * 2011-10-25 2015-07-23 Ajinomoto Co., Inc. Secretion production method for protein
EP2774982B1 (en) 2011-11-02 2017-06-21 Ajinomoto Co., Inc. Method for secreting and producing proteins
WO2013065869A1 (en) 2011-11-02 2013-05-10 Ajinomoto Co.,Inc. Method for secretory production of protein
JPWO2013118544A1 (ja) 2012-02-08 2015-05-11 味の素株式会社 タンパク質の分泌生産法
JP6309317B2 (ja) 2013-03-28 2018-04-11 株式会社半導体エネルギー研究所 発光素子及び化合物

Also Published As

Publication number Publication date
KR20170140351A (ko) 2017-12-20
JP6741000B2 (ja) 2020-08-19
US20180037918A1 (en) 2018-02-08
EP3287523B1 (en) 2022-06-29
WO2016171224A1 (ja) 2016-10-27
AU2016253217A1 (en) 2017-11-23
CN107532163A (zh) 2018-01-02
US10538798B2 (en) 2020-01-21
EP3287523A1 (en) 2018-02-28
WO2016171224A9 (ja) 2017-09-28
KR102619848B1 (ko) 2024-01-04
JPWO2016171224A1 (ja) 2018-02-22
EP3287523A4 (en) 2018-09-05
AU2016253217B2 (en) 2021-09-02

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