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DK3259364T3 - Fremgangsmåder til styring af proteasefremstilling - Google Patents

Fremgangsmåder til styring af proteasefremstilling Download PDF

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Publication number
DK3259364T3
DK3259364T3 DK16708433.4T DK16708433T DK3259364T3 DK 3259364 T3 DK3259364 T3 DK 3259364T3 DK 16708433 T DK16708433 T DK 16708433T DK 3259364 T3 DK3259364 T3 DK 3259364T3
Authority
DK
Denmark
Prior art keywords
methods
protease production
controlling
controlling protease
production
Prior art date
Application number
DK16708433.4T
Other languages
English (en)
Inventor
Marja Paloheimo
Susanna Mäkinen
Peter Punt
Kari Juntunen
Terhi Puranen
Jari Vehmaanperä
Original Assignee
Roal Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roal Oy filed Critical Roal Oy
Application granted granted Critical
Publication of DK3259364T3 publication Critical patent/DK3259364T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/14Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from fungi, algea or lichens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0055Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061Laccase (1.10.3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y110/00Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002Laccase (1.10.3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK16708433.4T 2015-02-20 2016-02-19 Fremgangsmåder til styring af proteasefremstilling DK3259364T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20155112 2015-02-20
PCT/FI2016/050108 WO2016132021A1 (en) 2015-02-20 2016-02-19 Methods for controlling protease production

Publications (1)

Publication Number Publication Date
DK3259364T3 true DK3259364T3 (da) 2020-09-07

Family

ID=55485006

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16708433.4T DK3259364T3 (da) 2015-02-20 2016-02-19 Fremgangsmåder til styring af proteasefremstilling

Country Status (8)

Country Link
US (3) US10883131B2 (da)
EP (1) EP3259364B1 (da)
CN (1) CN108138213A (da)
AU (1) AU2016221605B2 (da)
CA (1) CA2976021A1 (da)
DK (1) DK3259364T3 (da)
ES (1) ES2815628T3 (da)
WO (1) WO2016132021A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108138213A (zh) * 2015-02-20 2018-06-08 罗尔公司 控制蛋白酶产生的方法
CN110054684B (zh) * 2019-04-20 2021-07-20 中国农业科学院植物保护研究所 东亚飞蝗丝氨酸蛋白酶抑制剂serpin-5及其编码基因和应用
US20240279595A1 (en) 2020-07-31 2024-08-22 Biotalys NV Expression host
CN114686389B (zh) * 2020-12-25 2024-05-07 泰兴市东圣生物科技有限公司 一种增强vgbS基因转录水平的谷氨酰胺转氨酶高产菌株及其制备与发酵方法
CN113913413B (zh) * 2021-08-11 2023-10-27 上海雅心生物技术有限公司 一种耐盐的rpk突变体及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5296731A (en) * 1976-02-05 1977-08-13 Green Cross Corp:The Prophylactics and remedies of cyanomycosis
US5000939A (en) * 1984-06-12 1991-03-19 Colgate-Palmolive Company Dentifrice containing stabilized enzyme
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
ATE315638T1 (de) 1999-02-22 2006-02-15 Novozymes As Oxalacetat-hydrolase defiziente fungale wirtszellen
DK1266011T3 (da) 2000-03-14 2010-07-26 Novozymes As Svampetranskriptionsaktivator, der er anvendelig i fremgangsmåder til fremstilling af polypeptider
FI118339B (fi) 2004-09-21 2007-10-15 Ab Enzymes Oy Uusi lakkaasientsyymi ja sen käyttö
EP1799827A2 (en) * 2004-10-12 2007-06-27 DSMIP Assets B.V. Fungal transcriptional activators useful in methods for producing a polypeptide
PT1874927E (pt) 2005-04-29 2014-04-23 Ab Enzymes Oy Celulases melhoradas
CA2994320C (en) * 2005-12-22 2023-05-16 Roal Oy Treatment of cellulosic material and enzymes useful therein
WO2013102674A2 (en) 2012-01-05 2013-07-11 Novartis International Pharmaceutical Ltd. Protease deficient filamentous fungal cells and methods of use thereof
CN102787108B (zh) * 2012-07-27 2013-12-04 中国科学院微生物研究所 来源于里氏木霉的一个蛋白质及其基因的用途
EP2938727B1 (en) * 2012-12-28 2018-11-14 AB Enzymes GmbH Genes/genetic elements associated with mating impairment in trichoderma reesei qm6a and its derivatives and process for their identification
US10266814B2 (en) * 2014-06-02 2019-04-23 The Board Of Regents For Oklahoma State University Systems and methods for production and use of fungal glycosyl hydrolases
CN108138213A (zh) * 2015-02-20 2018-06-08 罗尔公司 控制蛋白酶产生的方法

Also Published As

Publication number Publication date
US20180037920A1 (en) 2018-02-08
AU2016221605B2 (en) 2019-12-05
US20240309419A1 (en) 2024-09-19
US20210102231A1 (en) 2021-04-08
WO2016132021A1 (en) 2016-08-25
AU2016221605A1 (en) 2017-09-07
EP3259364A1 (en) 2017-12-27
EP3259364B1 (en) 2020-07-01
ES2815628T3 (es) 2021-03-30
US10883131B2 (en) 2021-01-05
BR112017015180A2 (pt) 2018-01-16
CA2976021A1 (en) 2016-08-25
US12031168B2 (en) 2024-07-09
CN108138213A (zh) 2018-06-08

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