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DE69528706D1 - Gekoppeltes ampflikation- und ligationverfahren - Google Patents

Gekoppeltes ampflikation- und ligationverfahren

Info

Publication number
DE69528706D1
DE69528706D1 DE69528706T DE69528706T DE69528706D1 DE 69528706 D1 DE69528706 D1 DE 69528706D1 DE 69528706 T DE69528706 T DE 69528706T DE 69528706 T DE69528706 T DE 69528706T DE 69528706 D1 DE69528706 D1 DE 69528706D1
Authority
DE
Germany
Prior art keywords
ola
reaction
ampflication
coupled
ligation procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69528706T
Other languages
English (en)
Other versions
DE69528706T2 (de
Inventor
Faye Eggerding
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
PE Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PE Corp filed Critical PE Corp
Application granted granted Critical
Publication of DE69528706D1 publication Critical patent/DE69528706D1/de
Publication of DE69528706T2 publication Critical patent/DE69528706T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6862Ligase chain reaction [LCR]
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/81Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Seal Device For Vehicle (AREA)
  • Surgical Instruments (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Compounds Of Unknown Constitution (AREA)
DE69528706T 1994-08-19 1995-08-17 Gekoppeltes ampflikation- und ligationverfahren Expired - Lifetime DE69528706T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29268694A 1994-08-19 1994-08-19
PCT/US1995/010603 WO1996006190A2 (en) 1994-08-19 1995-08-17 Coupled amplification and ligation method

Publications (2)

Publication Number Publication Date
DE69528706D1 true DE69528706D1 (de) 2002-12-05
DE69528706T2 DE69528706T2 (de) 2003-06-12

Family

ID=23125755

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69528706T Expired - Lifetime DE69528706T2 (de) 1994-08-19 1995-08-17 Gekoppeltes ampflikation- und ligationverfahren

Country Status (7)

Country Link
US (2) US5912148A (de)
EP (1) EP0777749B1 (de)
JP (1) JP3102800B2 (de)
AT (1) ATE226983T1 (de)
CA (1) CA2195562A1 (de)
DE (1) DE69528706T2 (de)
WO (1) WO1996006190A2 (de)

Families Citing this family (248)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6280935B1 (en) * 1994-10-13 2001-08-28 Lynx Therapeutics, Inc. Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags
US6974666B1 (en) * 1994-10-21 2005-12-13 Appymetric, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
WO1997007235A2 (en) * 1995-08-14 1997-02-27 Abbott Laboratories All-in-one nucleic acid amplification assay
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
CA2199213C (en) * 1996-03-11 2007-04-17 John Wesley Backus Amplifying and detecting target nucleic acids using a post amplification incubation step
WO1997045559A1 (en) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
AUPO524897A0 (en) * 1997-02-21 1997-03-20 Johnson & Johnson Research Pty. Limited Method of amplifying specific nucleic acid target sequences
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6949370B1 (en) 1998-10-30 2005-09-27 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
CA2375106C (en) 1999-06-01 2014-08-19 Huda Y. Zoghbi Compositions and methods for the therapeutic use of an atonal-associated sequence for deafness, osteoarthritis, and abnormal cell proliferation
EP1190097A2 (de) * 1999-06-22 2002-03-27 Invitrogen Corporation Verbesserte primer und methoden zum nachweis und zur unterscheidung von nukleinsäuren
US20020119448A1 (en) * 1999-06-23 2002-08-29 Joseph A. Sorge Methods of enriching for and identifying polymorphisms
US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
US7455965B2 (en) 2000-04-14 2008-11-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
CA2410950A1 (en) * 2000-05-30 2001-12-06 Hans-Michael Wenz Methods for detecting target nucleic acids using coupled ligation and amplification
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) * 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
JP4310599B2 (ja) * 2000-07-05 2009-08-12 東洋紡績株式会社 塩基多型を検出する方法
WO2002053778A2 (en) * 2001-01-05 2002-07-11 Genomicfx, Inc. Method for relative quantification of attached nucleic acids
WO2004059289A2 (en) * 2001-05-22 2004-07-15 Epicentre Technologies Target-dependent transcription using deletion mutants of n4 rna polymerase
US7153656B2 (en) * 2001-09-11 2006-12-26 Los Alamos National Security, Llc Nucleic acid sequence detection using multiplexed oligonucleotide PCR
DE10147439B4 (de) * 2001-09-26 2014-01-30 Qiagen Gmbh Verfahren zur Isolierung von DNA aus biologischen Proben
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7510829B2 (en) * 2001-11-19 2009-03-31 Affymetrix, Inc. Multiplex PCR
US20030119004A1 (en) * 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
EP1572902B1 (de) 2002-02-01 2014-06-11 Life Technologies Corporation HOCHWIRKSAME siRNAS ZUR REDUZIERUNG DER EXPRESSION VON ZIELGENEN
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
JP4527984B2 (ja) 2002-02-01 2010-08-18 ライフ テクノロジーズ コーポレーション 増強された効力を有するオリゴヌクレオチド組成物
WO2003073233A2 (en) * 2002-02-26 2003-09-04 Pharmacia Corporation Sequence detection system calculator
FI20021325A0 (fi) * 2002-07-05 2002-07-05 Valtion Teknillinen Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
EP1636561B1 (de) 2002-07-15 2011-02-09 Board Of Regents, The University Of Texas System Screening kombinatorischer proteinbibliotheken mittels periplasmatischer expression
US20040023220A1 (en) 2002-07-23 2004-02-05 Lawrence Greenfield Integrated method for PCR cleanup and oligonucleotide removal
CN101912421A (zh) 2002-08-12 2010-12-15 杰能斯有限公司 涉及痘病毒和癌的方法及组合物
US7153658B2 (en) * 2002-09-19 2006-12-26 Applera Corporation Methods and compositions for detecting targets
AU2003272498A1 (en) * 2002-09-19 2004-04-08 Applera Corporation Fragmentation of dna
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
ATE399882T1 (de) * 2002-11-07 2008-07-15 Yoichi Matsubara Verfahren zum genmutationsnachweis
US20050053957A1 (en) * 2002-11-19 2005-03-10 Applera Corporation Polynucleotide sequence detection assays
WO2004046343A2 (en) * 2002-11-19 2004-06-03 Applera Corporation Polynucleotide sequence detection assays and analysis
JP2006508662A (ja) 2002-12-04 2006-03-16 アプレラ コーポレイション ポリヌクレオチドの多重増幅
US20040110134A1 (en) * 2002-12-05 2004-06-10 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US8043834B2 (en) * 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
WO2005001129A2 (en) * 2003-06-06 2005-01-06 Applera Corporation Mobility cassettes
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
CN1878866B (zh) 2003-07-25 2012-08-01 安比恩股份有限公司 用于从固定的样品中制备rna的方法和组合物
WO2005118862A2 (en) * 2004-04-30 2005-12-15 Applera Corporation Compositions, methods, and kits for (mis)ligating oligonucleotides
WO2006076017A2 (en) * 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7311794B2 (en) 2004-05-28 2007-12-25 Wafergen, Inc. Methods of sealing micro wells
US7785843B2 (en) * 2004-06-23 2010-08-31 Sequenom, Inc. Target-specific compomers and methods of use
RU2389798C2 (ru) 2004-09-14 2010-05-20 Дзе Риджентс Оф Дзе Юниверсити Оф Колорадо, Э Боди Корпорейт Способ лечения буциндололом, основанный на определении генотипа
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
KR20090004839A (ko) * 2005-09-07 2009-01-12 제네렉스, 인코포레이티드 Gm-csf를 발현하는 폭스바이러스를 사용한 전이성및/또는 전신 파종성 암의 전신 치료법
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
EP2839837B1 (de) 2006-09-15 2019-05-08 Children's Hospital of Eastern Ontario Research Institute Inc. Onkolytisches farmington rhabdovirus
US20080113349A1 (en) * 2006-11-03 2008-05-15 Pranvera Ikonomi Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay
US8067179B2 (en) 2006-11-30 2011-11-29 Research Development Foundation Immunoglobulin libraries
CA2685675C (en) 2007-05-01 2016-02-16 Research Development Foundation Immunoglobulin fc libraries
US8008010B1 (en) 2007-06-27 2011-08-30 Applied Biosystems, Llc Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor
KR20100098529A (ko) * 2007-12-13 2010-09-07 킴벌리-클라크 월드와이드, 인크. 폴리락트산 및 폴리에테르 공중합체를 함유하는 열가소성 조성물로부터 형성된 생분해성 섬유
US20090181390A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
WO2010115100A1 (en) 2009-04-03 2010-10-07 L&C Diagment, Inc. Multiplex nucleic acid detection methods and systems
US9587270B2 (en) 2009-06-29 2017-03-07 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
DK2464729T3 (en) 2009-08-14 2014-12-15 Epict Technologies Corp PROCEDURES, COMPOSITIONS AND KITS FOR PREPARING rRNA DEPLETED SAMPLES OR ISOLATING rRNA FROM SAMPLES
WO2011027966A2 (en) * 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
WO2011032088A1 (en) 2009-09-11 2011-03-17 Arca Biopharma, Inc. Polymorphisms in the pde3a gene
CA2774144C (en) 2009-09-14 2018-02-13 Jennerex, Inc. Oncolytic vaccinia virus combination cancer therapy
EP2980224B1 (de) 2009-10-21 2019-07-03 Brandeis University Kits zur analyse einzelstrangiger nukleinsäuresequenzen
MX337062B (es) 2009-12-10 2016-02-11 Ottawa Hospital Res Inst Rabdovirus oncolítico.
CA2822747A1 (en) 2009-12-23 2011-06-30 Arca Biopharma, Inc. Use of s-(6-nitro-oxi-hexahydro-furo[3,2-b]thioacetate in the treatment of cardiovascular disorders associated with oxide synthase dysfunction
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US8470261B2 (en) * 2010-05-06 2013-06-25 Ibis Biosciences, Inc. Integrated sample preparation systems and stabilized enzyme mixtures
CA2811294C (en) 2010-09-16 2016-06-28 Ibis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea
ES2689257T3 (es) * 2010-11-10 2018-11-12 Brandeis University Kit para la detección e identificación de micobacterias
WO2012092265A1 (en) 2010-12-27 2012-07-05 Ibis Biosciences, Inc. Nucleic acid sample preparation methods and compositions
CN103429258B (zh) 2011-01-04 2016-03-09 新罗杰公司 通过施用溶瘤痘苗病毒生成针对肿瘤抗原的抗体和生成肿瘤特异性补体依赖性细胞毒性
CA2826467C (en) 2011-02-07 2019-11-12 Research Development Foundation Engineered immunoglobulin fc polypeptides
MX2013009863A (es) 2011-02-28 2013-12-06 Univ Iowa Res Found Cambios de la hormona anti-mulleriana en el embarazo y prediccion de sexo y de resultados adversos en el embarazo.
EP2683835B1 (de) 2011-03-08 2017-01-11 King Abdullah University Of Science And Technology Molekulares biomarker-set zur früherkennung von eierstockkrebs
EP3150750B1 (de) 2011-04-08 2018-12-26 Prognosys Biosciences, Inc. Peptidkonstrukte und testsysteme dafür
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CA2872045A1 (en) 2011-06-08 2012-12-13 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
WO2013036799A2 (en) 2011-09-09 2013-03-14 Fred Hutchinson Cancer Research Center Methods and compositions involving nkg2d inhibitors and cancer
WO2013063308A1 (en) 2011-10-25 2013-05-02 University Of Massachusetts An enzymatic method to enrich for capped rna, kits for performing same, and compositions derived therefrom
US20140349858A1 (en) 2011-12-22 2014-11-27 Ibis Bioscience, Inc. Amplification of a sequence from a ribonucleic acid
US9334491B2 (en) 2011-12-22 2016-05-10 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
ES2626058T3 (es) 2011-12-22 2017-07-21 Ibis Biosciences, Inc. Cebadores y métodos de amplificación
US9803188B2 (en) 2011-12-22 2017-10-31 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
US9822417B2 (en) 2012-01-09 2017-11-21 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
US20130184165A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
EP2809783A2 (de) 2012-02-02 2014-12-10 Invenra, Inc. Hochdurchsatz-screen für biologisch aktive polypeptide
US20170260576A1 (en) * 2012-02-24 2017-09-14 Seegene, Inc. Td probe and its uses
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
WO2013166303A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Dna sequencing
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
JP6510978B2 (ja) 2012-10-16 2019-05-08 アボツト・モレキユラー・インコーポレイテツド 核酸を配列決定する方法および装置
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2014116721A1 (en) 2013-01-22 2014-07-31 The Arizona Board Of Regents For And On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
AU2014221143B2 (en) 2013-02-21 2019-02-07 Turnstone Limited Partnership Vaccine composition
WO2014152937A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
JP2016512437A (ja) 2013-03-14 2016-04-28 アボツト・モレキユラー・インコーポレイテツド 多重メチル化特異的増幅システムおよび方法
WO2014151511A2 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
US20160153029A1 (en) 2013-03-15 2016-06-02 Ibis Biosciences, Inc. Dna sequences to assess contamination in dna sequencing
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
EP3879012A1 (de) 2013-08-19 2021-09-15 Abbott Molecular Inc. Sequenzierungsbibliotheken der nächsten generation
WO2015042708A1 (en) 2013-09-25 2015-04-02 Bio-Id Diagnostic Inc. Methods for detecting nucleic acid fragments
SG11201602540XA (en) 2013-10-03 2016-04-28 Oklahoma Med Res Found Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
ES2885191T3 (es) 2013-10-17 2021-12-13 Illumina Inc Métodos y composiciones para preparar bibliotecas de ácidos nucleicos
CA2927752A1 (en) 2013-10-18 2015-04-23 The Regents Of The University Of Michigan Systems and methods for determining a treatment course of action
EP3080268A1 (de) 2013-12-09 2016-10-19 Illumina, Inc. Verfahren und zusammensetzungen zur gezielten nukleinsäuresequenzierung
WO2015095355A2 (en) 2013-12-17 2015-06-25 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
EP3151733B1 (de) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Zusammensetzungen und verfahren zur charakterisierung und diagnose von parodontaler erkrankung
AU2015280069B2 (en) 2014-06-23 2021-08-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
WO2015200541A1 (en) 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
JP2017521654A (ja) 2014-06-27 2017-08-03 アボット・ラボラトリーズAbbott Laboratories ヒトペギウイルス2(HPgV−2)を検出するための組成物および方法
EP3191607B1 (de) 2014-07-24 2019-08-21 Abbott Molecular Inc. Zusammensetzungen zum nachweis und zur analyse von mycobacterium tuberculosis
KR101559853B1 (ko) * 2014-10-06 2015-10-13 이진곤 골프티
WO2016130516A1 (en) 2015-02-09 2016-08-18 Research Development Foundation Engineered immunoglobulin fc polypeptides displaying improved complement activation
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
WO2016134021A1 (en) * 2015-02-20 2016-08-25 Rosalind Franklin University Of Medicine And Science Antisense compounds targeting genes associated with cystic fibrosis
EP3259602B9 (de) 2015-02-20 2021-05-19 Takara Bio USA, Inc. Verfahren zur schnellen präzisen abgabe, visualisierung und analyse von einzelzellen
US10525076B2 (en) 2015-02-20 2020-01-07 Rosalind Franklin University Of Medicine And Science Antisense compounds targeting genes associated with cystic fibrosis
EP3070178B1 (de) 2015-03-20 2020-02-12 Albert-Ludwigs-Universität Freiburg Verfahren zur diagnose von brustkrebs
ES2935860T3 (es) 2015-04-10 2023-03-13 Spatial Transcriptomics Ab Análisis de ácidos nucleicos múltiplex, espacialmente distinguidos de especímenes biológicos
KR20170136555A (ko) 2015-04-15 2017-12-11 더 제너럴 하스피탈 코포레이션 Lna-기반 돌연변이체 농축 차세대 서열분석 검정
ES2902125T3 (es) 2015-07-14 2022-03-25 Abbott Molecular Inc Purificación de ácidos nucleicos utilizando óxidos de cobre-titanio u óxidos de magnesio-titanio
EP3322483A4 (de) 2015-07-14 2019-01-02 Abbott Molecular Inc. Zusammensetzungen und verfahren zum nachweis von wirkstoffresistenter tuberkulose
US10526408B2 (en) 2015-08-28 2020-01-07 Research Development Foundation Engineered antibody FC variants
AU2016319110B2 (en) 2015-09-11 2022-01-27 The General Hospital Corporation Full interrogation of nuclease DSBs and sequencing (FIND-seq)
EP3356526B1 (de) 2015-09-30 2021-08-25 The General Hospital Corporation Umfassende in-vitro-meldung von spaltungsereignissen durch sequenzierung (circle-seq)
WO2017120531A1 (en) 2016-01-08 2017-07-13 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
US10626390B2 (en) 2016-02-08 2020-04-21 RGENE, Inc. Multiple ligase compositions, systems, and methods
CN109477127A (zh) 2016-03-14 2019-03-15 R基因股份有限公司 超热稳定的赖氨酸-突变型ssDNA/RNA连接酶
WO2018013509A1 (en) 2016-07-11 2018-01-18 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
WO2018013558A1 (en) 2016-07-12 2018-01-18 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
JP7075394B2 (ja) 2016-07-21 2022-05-25 タカラ バイオ ユーエスエー, インコーポレイテッド マルチウェルデバイスを用いたマルチz撮像及び分注
US11543417B2 (en) 2016-08-29 2023-01-03 Oslo Universitetssykehus Hf ChIP-seq assays
US11021738B2 (en) 2016-12-19 2021-06-01 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented DNA
AU2018213315A1 (en) 2017-01-26 2019-07-25 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
EP3354746B1 (de) 2017-01-30 2019-05-29 Gregor Mendel Institute of Molecular Plant Biology GmbH Neuartige spike-in-oligonukleotide zur normalisierung von sequenzdaten
EP3634992B1 (de) 2017-06-08 2024-03-27 The Brigham And Women's Hospital, Inc. Verfahren und zusammensetzungen zur identifizierung von epitopen
EP3642363A1 (de) 2017-06-20 2020-04-29 Bio-Rad Laboratories, Inc. Mda mit bead-oligonukleotid
WO2019002265A1 (en) 2017-06-26 2019-01-03 Universität Für Bodenkultur Wien NOVEL BIOMARKERS FOR THE DETECTION OF SENESCENT CELLS
JP7242644B2 (ja) 2017-09-20 2023-03-20 ガーダント ヘルス, インコーポレイテッド 体細胞および生殖細胞系統バリアントを鑑別するための方法およびシステム
US10907205B2 (en) 2017-11-02 2021-02-02 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
EP3746552B1 (de) 2018-01-31 2023-12-27 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur dekonvolution von partitionsbarcodes
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
WO2019213619A1 (en) 2018-05-04 2019-11-07 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
WO2019236478A1 (en) 2018-06-04 2019-12-12 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free nucleic acids
JP7466519B2 (ja) 2018-07-23 2024-04-12 ガーダント ヘルス, インコーポレイテッド 腫瘍遺伝子変異量を腫瘍割合およびカバレッジによって調整するための方法およびシステム
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN112970068A (zh) 2018-08-30 2021-06-15 夸登特健康公司 用于检测样品之间的污染的方法和系统
SG11202101400UA (en) 2018-08-31 2021-03-30 Guardant Health Inc Microsatellite instability detection in cell-free dna
JP2021534803A (ja) 2018-09-04 2021-12-16 ガーダント ヘルス, インコーポレイテッド 無細胞核酸試料におけるアレル不均衡を検出するための方法およびシステム
PL3814533T3 (pl) 2018-09-20 2022-01-31 Tamirna Gmbh Sygnatury mikro-rna do przewidywania dysfunkcji wątroby
US20210349088A1 (en) 2018-10-18 2021-11-11 Oklahoma Medical Research Foundation Biomarkers For A Systemic Lupus Erythematosus (SLE) Disease Activity Immune Index That Characterizes Disease Activity
US20200131566A1 (en) 2018-10-31 2020-04-30 Guardant Health, Inc. Methods, compositions and systems for calibrating epigenetic partitioning assays
EP3898969A1 (de) 2018-12-20 2021-10-27 Guardant Health, Inc. Verfahren, zusammensetzungen und systeme zur verbesserung der rückgewinnung von nukleinsäuremolekülen
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN113661249A (zh) 2019-01-31 2021-11-16 夸登特健康公司 用于分离无细胞dna的组合物和方法
WO2020176659A1 (en) 2019-02-27 2020-09-03 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free dna
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP3976822A1 (de) 2019-05-31 2022-04-06 Guardant Health, Inc. Verfahren und systeme zur verbesserung der patientenüberwachung nach der operation
US20220282326A1 (en) 2019-08-05 2022-09-08 Mission Bio, Inc. Method and Apparatus for Single-Cell Analysis for Determining a Cell Trajectory
EP3772543B1 (de) 2019-08-09 2023-09-27 Albert-Ludwigs-Universität Freiburg Verfahren zur diagnose von brustkrebs
EP3812472B1 (de) 2019-10-21 2022-11-23 Albert-Ludwigs-Universität Freiburg In-vitro-test zum profilieren der off-target-aktivität eines oder mehrerer zielspezifischer programmierbarer nukleasen in zellen (abnoba-seq)
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
CA3157560A1 (en) 2019-11-26 2021-06-03 Dustin Howard HITE Methods, compositions and systems for improving the binding of methylated polynucleotides
AU2020412766B2 (en) 2019-12-23 2024-09-19 10X Genomics, Inc. Methods for spatial analysis using RNA-templated ligation
US20210190770A1 (en) 2019-12-23 2021-06-24 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
WO2021163637A1 (en) 2020-02-13 2021-08-19 Zymergen Inc. Metagenomic library and natural product discovery platform
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
CN115768884A (zh) 2020-03-20 2023-03-07 使命生物公司 用于全基因组扩增的单细胞工作流程
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021222828A1 (en) 2020-04-30 2021-11-04 Guardant Health, Inc. Methods for sequence determination using partitioned nucleic acids
US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
EP4150113A1 (de) 2020-05-14 2023-03-22 Guardant Health, Inc. Nachweis von mangel an reparatur der homologen rekombination
EP4153776A1 (de) 2020-05-22 2023-03-29 10X Genomics, Inc. Räumliche analyse zur erkennung von sequenzvarianten
EP4414459A3 (de) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
AU2021283184A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207B1 (de) 2020-06-10 2024-09-25 10X Genomics, Inc. Verfahren zur bestimmung der position eines analyten in einer biologischen probe
EP4450639A2 (de) 2020-06-25 2024-10-23 10X Genomics, Inc. Räumliche analyse der dna-methylierung
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
EP4179111B1 (de) 2020-07-10 2024-04-03 Guardant Health, Inc. Verfahren zum nachweis von genomischen neuanordnungen unter verwendung zellfreier nukleinsäuren
WO2022020359A1 (en) 2020-07-24 2022-01-27 The Regents Of The University Of Michigan Compositions and methods for detecting and treating high grade subtypes of uterine cancer
US11946044B2 (en) 2020-07-30 2024-04-02 Guardant Health, Inc. Methods for isolating cell-free DNA
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
WO2022046947A1 (en) 2020-08-25 2022-03-03 Guardant Health, Inc. Methods and systems for predicting an origin of a variant
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022073012A1 (en) 2020-09-30 2022-04-07 Guardant Health, Inc. Compositions and methods for analyzing dna using partitioning and a methylation-dependent nuclease
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4267757A1 (de) 2020-12-23 2023-11-01 Guardant Health, Inc. Verfahren und systeme zur analyse methylierter polynukleotide
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
CA3210101A1 (en) 2021-03-05 2022-09-09 Katie Julia QUINN Methods and related aspects for analyzing molecular response
WO2022192889A1 (en) 2021-03-09 2022-09-15 Guardant Health, Inc. Detecting the presence of a tumor based on off-target polynucleotide sequencing data
EP4301870A1 (de) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
EP4347879A1 (de) 2021-06-03 2024-04-10 10X Genomics, Inc. Verfahren, zusammensetzungen, kits und systeme zur verbesserung der analyterfassung zur räumlichen analyse
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023220602A1 (en) 2022-05-09 2023-11-16 Guardant Health, Inc. Detecting degradation based on strand bias
US20240010742A1 (en) 2022-06-10 2024-01-11 Research Development Foundation Engineered fcriib selective igg1 fc variants and uses thereof
WO2024107599A1 (en) 2022-11-15 2024-05-23 Guardant Health, Inc. Method of predicting non-small cell lung cancer (nsclc) patient drug response or time until death or cancer progression from circulating tumor dna (ctdna) utilizing signals from both baseline ctdna level and longitudinal change of ctdna level over time
WO2024129672A1 (en) 2022-12-12 2024-06-20 The Broad Institute, Inc. Trafficked rnas for assessment of cell-cell connectivity and neuroanatomy
WO2024137682A1 (en) 2022-12-21 2024-06-27 Guardant Health, Inc. Detecting homologous recombination deficiences based on methylation status of cell-free nucleic acid molecules
WO2024154298A1 (ja) * 2023-01-19 2024-07-25 株式会社日立ハイテク 核酸定量方法および核酸定量用試薬

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5407769A (en) * 1989-07-28 1995-04-18 Canon Kabushiki Kaisha Magnetic toner having triaryl methyl organic resin
US5407796A (en) * 1991-01-04 1995-04-18 The Johns Hopkins University Cystic fibrosis mutation cluster
US5427932A (en) * 1991-04-09 1995-06-27 Reagents Of The University Of California Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using
DK0519338T3 (da) * 1991-06-20 1996-10-28 Hoffmann La Roche Forbedrede fremgangsmåder til nukleinsyreamplifikation
AU658962B2 (en) * 1991-11-15 1995-05-04 Igen, Inc. Rapid assays for amplification products
WO1993020236A1 (en) * 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
WO1994002648A1 (en) * 1992-07-21 1994-02-03 Imclone Systems Incorporated Gap-filling nucleic acid amplification and detection
US5508179A (en) * 1994-03-18 1996-04-16 Bio-Rad Laboratories, Inc. Use of deoxyribose nicotinamide adenine dinucleotide to enhance the specificity of NAD+ -dependent ligation reactions

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WO1996006190A2 (en) 1996-02-29
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US6130073A (en) 2000-10-10
US5912148A (en) 1999-06-15

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