CN211886631U - Dilution box convenient to mixing - Google Patents

Abstract

The utility model discloses a dilution box convenient to mixing belongs to biological and chemical experiment technical field. The effect of mixing uniformly in one time can be achieved by setting the ball body as the main body in the dilution place, 9 ml and 10 ml are distinguished by setting the bottom of the ball body of 9 ml and the upper part of the cylinder of 3 ml, the monitoring and the inspection of the dilution process are convenient, two rows of glass inner cores are arranged, 6 dilution cups in each row are used for the gradient dilution of two samples, serial numbers are arranged on the outer sides of the dilution cups to facilitate the orderly execution of dilution experiments, through resin box body and lid protection glass inner core reduce glass breakage probability, firm structure, avoid the dilution process to suffer air miscellaneous fungus pollution, satisfy the sample demand and dilute the experiment at the sample more than 1 ml, make 9 ml diluents and 1 ml sample mixing dilution process succinct, accurate, convenient, high-efficient, be fit for ten times of gradient dilution of blood sample, mould spore solution sample, yeast cell solution sample, various biological and chemical sample solution.

Description

Dilution box convenient to mixing

Technical Field

The utility model relates to a dilute the box, especially a dilute box convenient to liquid mixing belongs to biology and chemical experiment technical field.

Background

The gradient dilution of liquid is a common technique for biological and chemical experiments, such as blood samples, mold spore solution samples, yeast cell solution samples, tenfold gradient dilution of various biological and chemical sample solutions, etc., and the dilution in a test tube is a traditional technique used for hundreds of years. There are some disadvantages to completing the dilution in the tube. It is difficult to reach the bottom of the tube with 1000 or 200 microliter format pipette tips and pipettes. The test tube is of a slender type and is difficult to mix well. In order to avoid the liquid in the test tube from flowing out, a test tube rack and a test tube cover are needed, so that articles required by the experiment are too bloated, and the experiment process is complicated. Sterilized 9 ml of sterile water is susceptible to volume changes upon use due to evaporation. A interlinked dilution tube (ZL2010202088527) is convenient to dilute and mix, but has the defects of unstable structure, glass lack of protection and easy breakage, inconvenient transportation and packaging, tube cover lack, easy pollution by sundry bacteria in the air and difficulty in maintaining the sterile state after sterilization. Dilution cartridges (ZL2010202303792) facilitate dilution of liquid single samples below 1000 microliters, but some dilution procedures require sample volumes above 1000 microliters. A set of multiple dilution cartridges (ZL2010202304494) is suitable for parallel dilution of multiple samples, but only for sample volumes below 1000 microliters. Sit before the laboratory bench, perhaps when the aseptic technique platform is preceding to dilute the experiment, general naked eye only looks sideways at the control, and aforementioned two kinds dilute the box, because it is little to dilute the operating unit, the outward appearance is unanimous, looks sideways at difficult inspection, dilute the experiment operation process and confuse easily and forget the step, lead to the omission or the repetition of experimental step. Sometimes the dilution process requires a sample size of more than 1000 microliters. For example, in the case of microbial inverted plate experiments, more than 4000. mu.l of sample size is required for four replicates. When the volume of a sample to be diluted is more than 1 ml and less than 10 ml, the prior art can not simultaneously meet the requirements of convenient mixing and convenient sterilization.

Disclosure of Invention

The utility model aims at: in order to overcome the above-mentioned not enough of prior art, the utility model provides a dilution box convenient to mixing for the mixing and the dilution of experiment liquid sample are particularly suitable for ten times of gradient dilution of 9 ml diluent and 1 ml sample mixing.

The utility model provides a technical scheme that its technical problem adopted is: the utility model provides a dilute box convenient to mixing, including lid (1), box body (2), glass inner core (3), lid (1) is disconnected with box body (2), lid (1) and box body (2) are made by the transparent resin material of available 121 ℃ high pressure steam sterilization, glass inner core (3) are by A row dilute cup (4), B row dilute cup (5), barn door (6) are constituteed, A row dilute cup (4) and B row dilute cup (5) and are made by cuboid transparent glass material, set up 6 dilution bubble (7) that the specification is the same side by side respectively, lid (1) and box body (2) can with glass inner core (3) gomphosis, A row dilute cup (4) lateral surface marks respectively: a1, A2, A3, A4, A5 and A6, wherein the outer side surfaces of the dilution cups (5) in the row B are respectively marked as follows: b1, B2, B3, B4, B5 and B6, wherein the dilution bubble (7) is a hollow container and is shaped like a bottom sphere and an upper cylinder, the volume of the bottom sphere of the dilution bubble (7) is 9 milliliters, the diameter of the sphere is 2.58 centimeters, the diameter of the upper cylinder of the dilution bubble (7) is 1.2 centimeters, the volume of the upper cylinder of the dilution bubble (7) is 3 milliliters, and a cylinder light baffle (6) is made of sterilizable opaque glass.

The names and the purposes of the parts are as follows:

the box cover (1) is used for preventing the dilution cup from being polluted by air sundry bacteria;

the box body (2) is used for embedding the protective glass inner core (3) so as to reduce the possibility of glass breakage;

the glass inner core (3) is used for a mixing dilution experiment of 9 ml of liquid and 1 ml of sample, and is convenient to mix and sterilize;

the A row of dilution cups (4) are used for the dilution process of a sample;

the row B dilution cup (5) is used for the dilution process of the 2 nd sample;

the light barrier (6) is used for blocking the light rays of the dilution cups (4) in the row A and the dilution cups (5) in the row B;

dilution bubble (7) was used for each dilution step, the sphere volume was 9 ml, the sample and diluent were mixed to 10 ml, and the single dilution bubble volume was 12 ml.

The utility model has the advantages that: the utility model provides a dilution box convenient to mixing makes things convenient for the mixing of 1 ml sample and 9 ml diluent, and the great dilution process of being convenient for of glass material weight keeps firm upright state, conveniently monitors and dilutes the experimentation with the inspection and avoids omitting step and repeated step, conveniently sterilizes and keeps aseptic technique, and the cuboid structure is conveniently transported and is preserved, and the structure is succinct, dilutes portably. A ten-fold gradient dilution suitable for both samples. Such as dilution pour-plate experiments of fungi, etc.

Drawings

The present invention will be further explained with reference to the drawings and examples.

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the row A dilution cup (4) of the present invention;

fig. 3 is a schematic structural view of the row B dilution cup (5) of the present invention.

In the figure, 1, a box cover, 2, a box body, 3, a glass inner core, 4, A row of dilution cups, 5, B row of dilution cups, 6, a light barrier and 7, dilution bubbles.

Detailed Description

A row of dilution cups, a light barrier, a row of dilution cups, a box body and a box cover are respectively manufactured according to specifications and materials, and then the dilution boxes convenient for uniform mixing are assembled.

The utility model discloses a working process of diagram embodiment does: before diluting, prepare the glass container that has the lid, prepare the diluent, disinfect earlier the utility model discloses and diluent, then pour into 1 ml sample in diluting bubble (7) toward A1, move and get 9 ml sterilization diluent with 10 ml range pipettor, 1 ml sample of mixing draws 1 ml sample in following from A1 and changes over to A2, pours into 9 ml diluent mixing into, analogizes in proper order, until accomplishing the dilution process in the A6. Thus, at least 9 ml of samples with various dilution gradients are obtained, and the dilution process is simple, accurate and convenient.

Claims (1)

1. The utility model provides a dilute box convenient to mixing, characterized by includes lid (1), box body (2), glass inner core (3), lid (1) is disconnected with box body (2), lid (1) and box body (2) are made by the transparent resin material of available 121 ℃ high pressure steam sterilization, glass inner core (3) are by A row dilute cup (4), B row dilute cup (5), barn door (6) are constituteed, A row dilute cup (4) and B row dilute cup (5) and are made by cuboid transparent glass material, set up 6 dilution bubble (7) that the specification is the same side by side respectively, lid (1) and box body (2) can with glass inner core (3) gomphosis, A row dilute cup (4) lateral surface marks respectively: a1, A2, A3, A4, A5 and A6, wherein the outer side surfaces of the dilution cups (5) in the row B are respectively marked as follows: b1, B2, B3, B4, B5 and B6, wherein the dilution bubble (7) is a hollow container and is shaped like a bottom sphere and an upper cylinder, the volume of the bottom sphere of the dilution bubble (7) is 9 milliliters, the diameter of the sphere is 2.58 centimeters, the diameter of the upper cylinder of the dilution bubble (7) is 1.2 centimeters, the volume of the upper cylinder of the dilution bubble (7) is 3 milliliters, and a cylinder light baffle (6) is made of sterilizable opaque glass.

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