CN201809339U - Variety dilution box - Google Patents
Variety dilution box Download PDFInfo
- Publication number
- CN201809339U CN201809339U CN 201020230449 CN201020230449U CN201809339U CN 201809339 U CN201809339 U CN 201809339U CN 201020230449 CN201020230449 CN 201020230449 CN 201020230449 U CN201020230449 U CN 201020230449U CN 201809339 U CN201809339 U CN 201809339U
- Authority
- CN
- China
- Prior art keywords
- box
- dilution
- uncovered
- centrifuge tube
- water glass
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a set of variety dilution box which comprises a suction head box, a water glass box and a dilution box, and can be used for sterilizing by steam at 121 DEG C; the suction head box is characterized by being internally provided with a suction head frame for placing suction heads which respectively have volumes of 1000 microliters and 200 microliters; the number of suction head holes on the suction head frame is an optimal combination of the numbers of the suction heads which respectively have the volumes of 1000 microliters and 200 microliters and are needed by dilution; the water glass box is characterized by being internally provided with a water glass frame and a water glass, wherein the water glass frame is provided with a hole used for fixing the water glass; the dilution box is characterized by being internally provided with an uncovered centrifuge tube rack which is provided with a hole used for placing an uncovered centrifuge tube; and the uncovered centrifuge tube has a volume of 1.5 milliliters. The variety dilution box enables the preparation process and the operation process of simultaneously diluting a plurality of microbial samples to be simple and convenient, reduces the pollution risk, ensures the dilution steps not to be easily confused and enables the dilution time not to be limited by the standing time of sterile water.
Description
Affiliated technical field
The utility model relates to the cover dilution box that a plurality of samples of cover dilution box, especially a microorganism dilute simultaneously.
Background technology
At present, gradient dilution generally need be prepared test tube, test-tube stand, test tube plug, 1000 microlitre suction nozzles and suction nozzle box, 200 microlitre suction nozzles and suction nozzle box a plurality of sample of known microorganism the time.The a plurality of samples of existing this microorganism dilution simultaneously exist following point and shortcoming: test tube makes suction nozzle be difficult to draw the test tube bottom liquid too deeply, need the article of preparation too many, it is too big to take up room, operating process is numerous and diverse, Pollution risk is big, sample and dilution gradient thereof are obscured too much easily, placement produces than mistake because of diluting required sterilized water spontaneous evaporation smaller volume too for a long time easily, only is fit to now with the current.
Summary of the invention
The set-up procedure of gradient dilution and the above-mentioned deficiency of operating process when overcoming a plurality of sample of existing microorganism, the utility model provides a cover various dilution box, needed article were three boxes when the various dilution box of this cover was simplified a plurality of sample of dilution, the quantity of the required article of optimum combination, make the dilution Pollution risk little, be not easy to obscure, make the dilution blending process simpler, mixing is more abundant, does not produce error because of the sterilized water spontaneous evaporation.
The technical scheme that its technical problem that solves the utility model adopts is: the various dilution box of a cover, and it comprises: a suction nozzle box, a water tumbler box and a dilution box, can 121 ℃ of steam sterilizings.Before a plurality of diluted samples of microorganism, only need in the suction nozzle box, load onto 1000 microlitre suction nozzles and 200 microlitre suction nozzles, inject water, toward the box-packed upward uncovered centrifuge tube of dilution, stand-by the getting final product of sterilizing toward water tumbler.Move into 0.1 ml sample toward diluting whenever to ranked first in the uncovered centrifuge tube in the box with 200 microlitre suction nozzles earlier during dilution, then with in the uncovered centrifuge tube that sample is housed, moving into 0.9 ml sterile water the water tumbler of 1000 microlitre suction nozzles in the water tumbler box, reach the purpose of the dilution the first step, the later steps in sequence of dilution is analogized.Because measure by pipettor and suction nozzle when sterilized water is dilution, thereby can not produce error because of the sterilized water evaporated volume changes, dilution time is not limited by storage period.
The feature of suction nozzle box is the suction nozzle frame that interior dress can be shelved 1000 microlitre suction nozzles and 200 microlitre suction nozzles.Because 1000 microlitre suction nozzles in use do not contact sample, if do not have contaminatedly just can reuse, therefore need quantity to reduce than conventional art, 200 microlitre suction nozzles are used for pipetting sample because of needs, can only use once, therefore need quantity to increase than conventional art.Two kinds of suction nozzle optimum combinations are contained in the box can simplify dilution.
The feature of water tumbler box is interior dress drinking cup holder and water tumbler, and the fixedly hole of water tumbler is arranged on the drinking cup holder.Be convenient to pipettor behind the water tumbler splendid attire water and draw the interior water of water tumbler, each step needs the aseptic water yield little with 0.1 ml sample and 0.9 ml sterile water during dilution, can pass through the water tumbler splendid attire.In a single day water in the water tumbler pollute the dilution that will have influence on the back, and by increasing water tumbler quantity, the frequent water tumbler of changing can reduce this contamination of heavy when drawing sterilized water selection water tumbler.
The feature of dilution box is interior dress uncovered centrifuge tube shelf, and the hole that can shelve the uncovered centrifuge tube is arranged on the uncovered centrifuge tube shelf, and the volume of uncovered centrifuge tube is 1.5 milliliters.The uncovered centrifuge tube can be the centrifuge tube that cuts off 1.5 milliliters of specifications can sterilizing of lid, each step of dilution need take the volume of 1 milliliter of centrifuge tube, thereby the centrifuge tube of 1.5 milliliters of specifications is fit to dilution, and make pipettor easily draw uncovered centrifuge tube bottom liquid, thereby use test tube easy more in the past.Because centrifuge tube is more much smaller than existing test tube volume commonly used, and the uncovered centrifuge tube shelf is much lighter than test-tube stand, therefore makes dilution easier.
The beneficial effects of the utility model are: set-up procedure and operating process that a plurality of microbiological specimens are diluted simultaneously are easy, and Pollution risk diminishes, and dilution step is not easy to obscure, and dilution time is not limited by sterilized water storage period.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 is the organigram of the various dilution box embodiment of a cover.
Fig. 2 is the longitudinal section organigram of the various dilution box embodiment of a cover.
1. water tumbler boxes among the figure, 2. water tumbler hole, 3. drinking cup holder, 4. suction nozzle box, 5.1000 microlitre suction nozzle holes, 6.200 microlitre suction nozzle hole, 7. suction nozzle frame, 8. dilution box, the 9. centrifugal pore of uncovered, 10. dilution box internal frame, 11. water tumbler, 12.200 microlitre suction nozzles, 13.1000 microlitre suction nozzles, 14. uncovered centrifuge tubes.
Embodiment
In Fig. 1, place drinking cup holder (3) in the water tumbler box (1), fixedly water tumbler (11) of water tumbler hole (2) is arranged, drinking cup holder (3) and water tumbler box (1) tight engagement on the drinking cup holder (3).Place suction nozzle frame (7) in the suction nozzle box (4), have 1000 microlitre suction nozzle holes (5) and 200 microlitre suction nozzle holes (6) can shelve 1000 microlitre suction nozzles (13) and 200 microlitre suction nozzles (12) on the suction nozzle frame (7), suction nozzle frame (7) and suction nozzle box (4) tight engagement.Place dilution box internal frame (10) in the dilution box (8), on the dilution box internal frame (10) uncovered centrifuge tube hole (9) is arranged, the centrifugal pore of uncovered (9) can be shelved uncovered centrifuge tube (14), dilution box internal frame (10) and dilution box (8) tight engagement.
Water tumbler box (1), suction nozzle box (4), dilution box (8), drinking cup holder (3), suction nozzle frame (7), dilution box internal frame (10), uncovered centrifuge tube (14) are made by resin material that can the high-temperature steam sterilization, and water tumbler (11) is made by glass.
Claims (4)
1. one overlaps various dilution box, and it comprises: a suction nozzle box, a water tumbler box and a dilution box, and can 121 ℃ of steam sterilizings.
2. the various dilution box of a cover according to claim 1, it is characterized in that: the feature of suction nozzle box is the suction nozzle frame that interior dress can be shelved 1000 microlitre suction nozzles and 200 microlitre suction nozzles.
3. the various dilution box of a cover according to claim 1, it is characterized in that: the feature of water tumbler box is interior dress drinking cup holder and water tumbler, and the fixedly hole of water tumbler is arranged on the drinking cup holder.
4. the various dilution box of a cover according to claim 1, it is characterized in that: the feature of dilution box is interior dress uncovered centrifuge tube shelf, and the hole that can shelve the uncovered centrifuge tube is arranged on the uncovered centrifuge tube shelf, and the volume of uncovered centrifuge tube is 1.5 milliliters.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201020230449 CN201809339U (en) | 2010-06-21 | 2010-06-21 | Variety dilution box |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201020230449 CN201809339U (en) | 2010-06-21 | 2010-06-21 | Variety dilution box |
Publications (1)
Publication Number | Publication Date |
---|---|
CN201809339U true CN201809339U (en) | 2011-04-27 |
Family
ID=43892278
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 201020230449 Expired - Fee Related CN201809339U (en) | 2010-06-21 | 2010-06-21 | Variety dilution box |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN201809339U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108226494A (en) * | 2018-01-17 | 2018-06-29 | 郑州中道生物技术有限公司 | Porcine reproductive and respiratory syndrome virus ELISA antibody assay kits |
-
2010
- 2010-06-21 CN CN 201020230449 patent/CN201809339U/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108226494A (en) * | 2018-01-17 | 2018-06-29 | 郑州中道生物技术有限公司 | Porcine reproductive and respiratory syndrome virus ELISA antibody assay kits |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110427 Termination date: 20130621 |