CN209052695U - A kind of laboratory anaerobic fermentation easy device - Google Patents
A kind of laboratory anaerobic fermentation easy device Download PDFInfo
- Publication number
- CN209052695U CN209052695U CN201821579476.5U CN201821579476U CN209052695U CN 209052695 U CN209052695 U CN 209052695U CN 201821579476 U CN201821579476 U CN 201821579476U CN 209052695 U CN209052695 U CN 209052695U
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- CN
- China
- Prior art keywords
- fermentation
- laboratory
- anaerobic fermentation
- flask
- easy device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 75
- 230000004151 fermentation Effects 0.000 claims abstract description 51
- 229910001220 stainless steel Inorganic materials 0.000 claims description 9
- 239000010935 stainless steel Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 239000012188 paraffin wax Substances 0.000 claims description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 2
- 239000007789 gas Substances 0.000 abstract description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 5
- 239000001301 oxygen Substances 0.000 abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 238000004500 asepsis Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of laboratory anaerobic fermentation easy device, including fermentation flask, the fermentation flask is equipped with closing device, and the closing device is equipped with fermentation bung and sampler.The laboratory anaerobic fermentation easy device can guarantee that metastable oxygen-free environment, the gas that fermentation process generates can be discharged in time, can guarantee and fast sample in entire fermentation process and do not enter oxygen, be able to satisfy the anaerobic fermentation demand in laboratory.
Description
Technical field
The utility model relates to a kind of installation for fermenting, in particular to a kind of laboratory anaerobic fermentation easy device.
Background technique
Anaerobic fermentation refers to organic matter under anaerobic, is decomposed by anaerobic bacteria or facultative anaerobic bacteria and utilizes and generate
The process of certain metabolin.The presence of oxygen is not needed in anaerobic fermentation process, while fermentation process may discharge CO2Equal gas
Body.Therefore, it when carrying out anaerobic fermentation experiment, needs O in Zymolysis Equipment2It is cleared, while may need can be carried out the row of gas
Out.In addition, needing to be sampled fermentation process analysis during the experiment, it is therefore desirable to which anaerobic fermentation equipment can conveniently take
Sample.
Use for laboratory mainly has anaerobism pipe and anaerobic fermentation tank etc. in the device of anaerobic fermentation at present.Anaerobism pipe is one to finish
Hermetically sealed device, but anaerobism pipe is generally only suitable for anaerogenic bacterium of fermenting, and operates more demanding.Although anaerobic fermentation tank
The shortcomings that anaerobism pipe can be overcome, but expensive, complicated for operation, higher operating costs.
Utility model content
The main purpose of the utility model is to provide a kind of laboratory anaerobic fermentation easy device, can effectively solve to carry on the back
The problems in scape technology.
To achieve the above object, the technical solution that the utility model is taken are as follows:
A kind of laboratory anaerobic fermentation easy device, including fermentation flask, the fermentation flask are equipped with closing device, the sealing
Device is equipped with fermentation bung and sampler, and the sampler is one and interts the stainless steel into fermentation flask from rubber stopper
Needle tubing, stainless steel needle tubing upper end are equipped with syringe filter, and the syringe filter upper end is equipped with syringe.
Further, the closing device is rubber stopper, and is sealed between rubber stopper and fermentation flask by paraffin, is guaranteed close
Seal effect.
Further, the fermentation bung is sealed using liquid.
Further, the liquid is the sulfuric acid solution of sterile water or 50% volumetric concentration.
Further, the syringe filter aperture is 0.22 μm or 0.45 μm.
Further, the fermentation flask is vial.
Compared with prior art, the utility model has the following beneficial effects:
A kind of laboratory anaerobic fermentation easy device can guarantee metastable oxygen-free environment, what fermentation process generated
Gas can be discharged in time, can guarantee and fast sample in entire fermentation process and do not enter oxygen, be able to satisfy the anaerobism in laboratory
Fermentation demand.
Detailed description of the invention
Fig. 1 is the utility model laboratory anaerobic fermentation easy device overall structure diagram.
In figure: 1, fermentation flask；2, closing device；3, fermentation bung；4, sampler；5, stainless steel needle tubing；6, needle type filtration
Device；7, syringe.
Specific embodiment
To be easy to understand the technical means, creative features, achievement of purpose, and effectiveness of the utility model, below
In conjunction with specific embodiment, the utility model is further described.
As shown in Figure 1, a kind of laboratory anaerobic fermentation easy device, including fermentation flask 1, the fermentation flask 1 are vial,
The fermentation flask 1 is equipped with closing device 2, and the closing device 2 is rubber stopper, and passes through paraffin between rubber stopper and fermentation flask 1
Sealing, the closing device 2 are equipped with fermentation bung 3 and sampler 4, and the fermentation bung 3 is sealed using liquid, the liquid
Body is sterile water, and the sampler 4 is one from the interspersed stainless steel needle tubing 5 into fermentation flask 1 of rubber stopper, described stainless
5 upper end of draw point pipe is equipped with 0.22 μm of syringe filter 6, and 6 upper end of syringe filter is equipped with syringe 7.
It should be noted that a kind of working principle of laboratory anaerobic fermentation easy device of the utility model is fermentation bung 3
Sterile water is provided with as sealing solution, closing device 2(rubber stopper) and fermentation flask 1 between further sealed with paraffin, fermentation bung
3, it is also further sealed with paraffin between sampler 4 and rubber stopper, to reach better seal effect.Fermentation bung 3 protrudes into hair
1 upper space of ferment bottle, stainless steel needle tubing 5 access in fermentation flask 1 and are immersed in fermentation liquid level or less；
The gas generated in fermentation process can escape air by fermentation bung 3 and cannot be introduced into, therefore can guarantee to ferment
The microorganism in environment can be effectively prevented to enter in fermentation flask 1 again while equipment and ambient pressure consistency；
In order to further ensure anaerobic state in fermentation flask 1 passes through syringe filter after fermentation inoculation and after sampling
6 and stainless steel needle tubing 5 nitrogen or mixed gas (N are blown into fermentation flask 12、H2、CO2) replacement of oxygen is clean.
When being sampled in fermentation process, syringe filter 6 is removed, hair needed for being drawn from fermentation flask 1 with asepsis injector 7
Zymotic fluid loads onto sterile syringe filter 6 after sampling, then pass through syringe filter 6 and stainless steel needle tubing 5 to fermentation flask 1
In be blown into nitrogen or mixed gas (N2、H2、CO2), then sucking nitrogen or mixed gas (N are packed into syringe filter 62、H2、
CO2) asepsis injector 7 is sealed, guarantee that no oxygen enters in fermentation flask 1 in sampling and fermentation process.
The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above.Current row
The technical staff of industry is described in above embodiments and description it should be appreciated that the present utility model is not limited to the above embodiments
Only illustrate the principles of the present invention, on the premise of not departing from the spirit and scope of the utility model, the utility model is also
It will have various changes and improvements, these various changes and improvements fall within the scope of the claimed invention.The utility model
Claimed range is defined by the appending claims and its equivalent thereof.
Claims (6)
1. a kind of laboratory anaerobic fermentation easy device, it is characterised in that: including fermentation flask (1), the fermentation flask (1) is equipped with envelope
Mouthful device (2), the closing device (2) are equipped with fermentation bung (3) and sampler (4), the sampler (4) for one from
The interspersed stainless steel needle tubing (5) into fermentation flask (1) of rubber stopper, stainless steel needle tubing (5) upper end is equipped with needle type filtration
Device (6), syringe filter (6) upper end are equipped with syringe (7).
2. a kind of laboratory anaerobic fermentation easy device according to claim 1, it is characterised in that: the closing device
It (2) is rubber stopper, and sealed between rubber stopper and fermentation flask (1) by paraffin.
3. a kind of laboratory anaerobic fermentation easy device according to claim 1, it is characterised in that: the fermentation bung (3)
It is sealed using liquid.
4. a kind of laboratory anaerobic fermentation easy device according to claim 3, it is characterised in that: the liquid is sterile
The sulfuric acid solution of water or 50% volumetric concentration.
5. a kind of laboratory anaerobic fermentation easy device according to claim 1, it is characterised in that: the syringe filter
(6) aperture is 0.22 μm or 0.45 μm.
6. a kind of laboratory anaerobic fermentation easy device according to claim 1, it is characterised in that: the fermentation flask (1)
For vial.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821579476.5U CN209052695U (en) | 2018-09-27 | 2018-09-27 | A kind of laboratory anaerobic fermentation easy device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821579476.5U CN209052695U (en) | 2018-09-27 | 2018-09-27 | A kind of laboratory anaerobic fermentation easy device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209052695U true CN209052695U (en) | 2019-07-02 |
Family
ID=67049722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821579476.5U Expired - Fee Related CN209052695U (en) | 2018-09-27 | 2018-09-27 | A kind of laboratory anaerobic fermentation easy device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209052695U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110343610A (en) * | 2019-08-16 | 2019-10-18 | 贵州大学 | A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide |
| CN111534421A (en) * | 2020-05-14 | 2020-08-14 | 中国科学院沈阳应用生态研究所 | Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device |
-
2018
- 2018-09-27 CN CN201821579476.5U patent/CN209052695U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110343610A (en) * | 2019-08-16 | 2019-10-18 | 贵州大学 | A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide |
| CN111534421A (en) * | 2020-05-14 | 2020-08-14 | 中国科学院沈阳应用生态研究所 | Anaerobic culture device, soil anaerobic culture method and sampling method of anaerobic culture device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220831 Address after: No. 68, Shunxing Road, Xishunhe Town, Hongze County, Huaian City, Jiangsu Province 223100 Patentee after: Hongze County Fushun Modern Ecological Fishery Co.,Ltd. Address before: 223399 No. 111, Changjiang West Road, Huaiyin District, Huai'an City, Jiangsu Province Patentee before: HUAIYIN NORMAL University |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190702 |