CN204550627U - A kind of air ventilation device for cell cultures - Google Patents
A kind of air ventilation device for cell cultures Download PDFInfo
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- CN204550627U CN204550627U CN201520134456.7U CN201520134456U CN204550627U CN 204550627 U CN204550627 U CN 204550627U CN 201520134456 U CN201520134456 U CN 201520134456U CN 204550627 U CN204550627 U CN 204550627U
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Abstract
The utility model discloses a kind of air ventilation device for cell cultures.Described device is made up of cell culture environment container, buoyancy carrier, cartridge, gaseous fraction input module, gaseous equilibrium bag, vacuum elements, manometer and connecting tube.The basic using method of described device is: under the condition of cell culture environment container closure and isothermal, directly carries out water in container-gas displacement, or water-gas displacement is carried out on the basis of gas-gas displacement in container.The design of buoyancy carrier and cartridge can make the residual gas phase space after placing culture vessel in described environmental chamber very little, directly carry out water-gas displacement and can prepare mixed gas needed for most cells culture environment in container, also significantly reduce the burden of gas-gas displacement, save time province's gas, simple operation.The utility model provides a series of formula, replaces the enforcement of gentle-gas displacement, can meet the quantitative requirement of gaseous fraction in cell culture environment better for water-gas.
Description
Technical field
The utility model relates to technical field of experiment equipment, is specially a kind of air ventilation device being applicable to cell cultures.
Background technology
Cell cultures makes its growth and breeding, and maintains a kind of technology of its structure and function under generally referring to and the cell that in-vivo tissue takes out being placed in in-vitro simulated physiological environment.In this simulated environment, suitable gas concentration is the prerequisite of cells in vitro existence.Cell cultures is also the important means of gas physiopathology research, and tested gas is as the component of mixed gas in cell cultures gaseous environment.
Encloses container is usually used in cell cultures, and culture vessel is placed in container, the air displacement in container is become the mixed gas of certain component proportion, for setting up cell cultures gaseous environment.Gas displacement in this encloses container has again different concrete grammars and the device that matches.One method is continued to pass into container by mixed gas, and the air in container is discharged.Such as, in patent " a kind of hypobaric hypoxia cell culture apparatus " (ZL201420550339.4), exactly mixed gas (2% oxygen, 5% carbonic acid gas, 93% nitrogen) is continued to pass into cultivation air chamber, thus realize gas displacement.This method is time-consuming takes gas, and needs prewired mixed gas.Another kind method is first extracted out by the original gas fraction in container, then component gas and tested gasometry is filled with container.This method requires to bleed and inflate quantitatively to carry out, but its quantitative problem is not yet well solved.
A kind of method that patent " the gentle pressure adjustable cell culture case of a kind of gas concentration " (ZL 200810031070.8) provides is: the water adding certain volume in container, cell culture vessel is placed in the gas-phase space above the water surface by support; Quantitatively extracted out by water in container, gaseous fraction is quantitatively filled with container simultaneously, sets up cell cultures gaseous environment by the displacement of equal-volume water/gas.But, for requiring O
2the cell culture experiments that concentration is extremely low or very high, need by O contained by air
2or N
2almost cement out completely, because the air capacity in water surface superjacent air space is comparatively large, need many wheels drawing water-air inlet and-air inlet of drawing water after water filling again, operate the used time still longer.
Therefore, the apparatus and method that encloses container inner cell cultivation gaseous environment is set up need to improve simplicity and practicality.
Summary of the invention
The deficiency that the utility model exists for above-mentioned gas in closed vessel displacement apparatus and method, provides a kind of simple and practical air ventilation device and using method thereof.
The utility model adopts following technical scheme:
Be replaced into an air ventilation device for core technology with gauge water-gas displacement and firm gas-gas, this device is filled with assembly, vacuum elements, manometer, gaseous equilibrium bag and connecting tube is formed by cell culture environment container (hereinafter referred to as environmental chamber), buoyancy carrier, cartridge, gaseous fraction.
Described environmental chamber be cover plate have round-ended cylinder, the equal diameters of base plate and cover plate, be all greater than outside diameter of cylinder 2 ~ 3cm.There are a water service pipe in cylinder base plate central authorities, and it is equipped with water valve.Cover plate there are two ventpipes, the equal connecting tee air valve in cylinder outer end of pipe.The lower panel of cover plate has a circular groove, groove external diameter is slightly larger than outside diameter of cylinder, and well width is slightly larger than cylindrical wall thickness, and groove is embedded with silica gel sealing ring.On base plate and cover plate, the circular hole that all equidistant distribution number order is equal on the circumferential line of outer rim 1 ~ 2cm, the end opening in each hole of base plate is bonded with nut.Screw screws in the nut on base plate under corresponding circular hole downwards from the circular hole cover plate, and mouth cylinder wall is embedded in cover plate circular groove, and cover plate is held on mouth cylinder.On the inwall of described cylinder, on the contour of nozzle circle several centimetres, be equidistantly bonded with 4 support bars.
Described buoyancy carrier is disc closed enclosure, and diameter is slightly less than barrel bore, and it is recessed that upper Middle face has square box shaped or discoidal culture vessel to place, and for placing cell culture vessel, it is equal with the height of the culture vessel of placement that culture vessel places the recessed degree of depth; Described buoyancy carrier is placed in cylinder, is positioned on described support bar, can floats on liquid level, and when liquid level drops under support bar, buoyancy carrier is shelved on it.
Described cartridge is used for culture vessel described in filling and places space between recessed sidewall and culture vessel, hollow and airtight.Separately there is the cartridge for oxygen-free environment cell cultures, opening wide above it, for placing reductor.
Described gaseous fraction input module is composed in series by bladder tank, gas filter and three-way air valve, and three-way air valve is connected with a ventpipe on described cover plate, the another port of this three-way air valve connects described gaseous equilibrium bag.
Described vacuum elements is connected to form by hand-operating vacuum-pump and three-way air valve, and three-way air valve is connected with another root ventpipe on described cover plate, and the another port of this three-way air valve connects described manometer.
The basic using method of the utility model air ventilation device is: add water in described environmental chamber, is placed in container by buoyancy carrier, then is placed on carrier by culture vessel, covers tightly cover plate.Under isothermal conditions, discharge water in described environmental chamber, when container internal gas pressure is equal with around atmospheric pressure, then have the gaseous fraction of equal volume to enter container from described bladder tank.By this equal-volume water-gas displacement, various gaseous fraction is inputted described environmental chamber, in container, prepares mixed gas needed for cell culture environment.For O
2the cell culture experiments that concentration is extremely low or very high, then add water as much as possible in described environmental chamber, and gas-phase space in container is reduced to minimum, advanced promoting the circulation of qi-gas displacement, then carry out water-gas displacement.
Described gas-gas displacement is under isothermal conditions, and be quantitatively with reference to index with air pressure, bleed in described environmental chamber, make container internal gas pressure lower 1/2, then the gas volume extracted out equals 1/2 of gas in container amount; Then in container, be filled with unstripped gas, level before container internal gas pressure being returned to bleed, then the material gas quantity be filled with is equal with withdrawing gas amount.So carry out once bleed and once inflation is counted one and is taken turns and bleed-inflate.Take turns or take turns more bleeding by one-inflate (N
2or O
2), make constituent of air N in environmental chamber
2or O
2increase or reduce to certain value.
Further, the use of described air ventilation device is divided into following four kinds of situations.
1.2%≤O
2the cell culture experiments (alone water-gas displacement) of volume fraction≤80%.
1) calculating of water in environmental chamber-gas displacement desired gas volume components
The gas component volumes that need add in described environmental chamber is calculated by formula 1
v gc:
In formula,
v gc: water-gas displacement need add arbitrary gas component volumes of displacement in described environmental chamber, comprises
v o2,
v cO2,
v n2,
v gx.Symbol subscript O
2and CO
2i.e. literal implication own; For the purpose of practicality, N
2general expression N
2with air contained by other rare gas element; Gx represents non-O
2non-CO
2non-N
2gas (in this specification sheets in other symbol, these lower target implications are also same);
f c: gas-phase space desired gas volume components mark in described environmental chamber during cell cultures, comprises
f c-O2,
f c-CO2,
f c-N2,
f c-Gx,
f c-Va, all
f csum equals 1.
f c-Varepresent the volume fraction of water vapor in cell culture environment.
f c-N2with meeting other
f crear fixed, namely
f c-N2=1-(
f c-O2+
f c-CO2+
f c-Va+
f c-GX), wherein
f c-Vaduring value 0.06(37 DEG C);
f i: the volume fraction of gaseous fraction contained by air in water-front described environmental chamber of gas displacement, comprises
f i-O2,
f i-CO2,
f i-N2,
f i-Va, all
f isum equals 1.Under general experimental situation condition, mainly with
f i-Vasize, other
f ithere is small size variation.For the purpose of practicality,
f i-O2,
f i-CO2,
f i-N2,
f i-Vasimple value be 0.20,0.00,0.78,0.02 successively;
v c: the volume of gas-phase space in described environmental chamber during cell cultures,
v c=
v t-(
v d+ v v+ v m+ v w-c), wherein,
v trepresent the cubic capacity of environmental chamber;
v drepresent the volume of described buoyancy carrier;
v vrepresent the volume of cell culture vessel;
v mrepresent the cumulative volume of substratum in cell culture vessel;
v w-cthe volume of water in described environmental chamber when representing cell cultures;
v i: the volume of gas-phase space in water-front described environmental chamber of gas displacement, in order to utilize O contained by air in environmental chamber
2, it is suitable to retain
v i, be able to meet
f c-O2's
v i=
f c-O2.
v c/
f i-O2, by amount of water in environmental chamber before water-gas displacement
v w-inumber, reach retain suitable
v iobject,
v w-i=
v t-(
v d+
v v+
v m+
v i);
t i: surrounding environment (in laboratory) absolute temperature (K);
t c: absolute temperature (K=273) in described environmental chamber during cell cultures.
2) operation steps of water-gas displacement.
1. formula is used
v i=
f c-O2.
v c/
f i-O2needed for calculating
v i, by what calculate
v isubstitute into
v w-i=
v t-(
v d+
v v+
v m+
v i), calculate
v w-i, in described environmental chamber, add volume is
v w-isterilized water.
2. buoyancy carrier is put into described environmental chamber, culture vessel is placed on carrier, cover tightly described container cover plate.
3. make described environmental chamber be communicated with certain gaseous fraction bladder tank, make environmental chamber be communicated with described manometer simultaneously, discharge water in environmental chamber, in described bladder tank, gas enters container, when the volume that discharges water reaches this gaseous fraction
v gctime, stop discharging water; If now described environmental chamber internal gas pressure is lower than around atmospheric pressure, the described bladder tank of appropriateness extruding, makes gas in bag continue to enter in container, equal with around atmospheric pressure to container internal gas pressure.
4. by step 3. repetitive operation, required various gaseous fraction is filled with described environmental chamber.
5. take off described gaseous fraction input module and manometer, described gaseous equilibrium bag is communicated with environmental chamber, described environmental chamber is moved in constant incubator.
6., after cell cultures carries out 30min, extract 10mL gas out from described environmental chamber, detected components concentration, the difference if any concentration of component detected value and its set(ting)value exceedes allowed band, and available gas syringe injects a small amount of component gas in container, corrects.
2. 2% > O
2the cell culture experiments (coupling gas-gas displacement and water-gas displacement) of volume fraction > 80%.
1) bleed needed for the displacement of gas-gas-inflated wheel number is gentle-gas displacement after the calculating of gas component volumes mark in environmental chamber
Bleed-inflate and generally comprise bleed-fill nitrogen and-oxygenation of bleeding, the former makes to subtract oxygen nitrogen pick-up in described environmental chamber, for low-oxygen environment cell cultures; The latter is used for oxygenation in described environmental chamber and subtracts nitrogen, for oxygen environment cell cultures.
Carry out environmental chamber to bleed-fill nitrogen, require O in container
2volume fraction reduces to
f c-O2(
v c/
v i); For the purpose of simplification, by changing the volume of water adding described environmental chamber
v w-i, will
v c/
v ibe adjusted to 10, when namely container inner cell is cultivated, the volume of gas-phase space is 10 times of the volume of gas-phase space before water-gas is replaced, and calculates required nitrogen wheel number of bleeding-fill by formula 2
r l-O2, calculate by formula 3
rwheel to be bled-fills after nitrogen O in described environmental chamber
2volume fraction
f pr-O2:
If calculate
r l-O2be positive integer, directly take turns number with its nitrogen of bleeding-fill as actually operating
r; If calculate
r l-O2being positive mark, is then the minimum positive integer larger than it nitrogen wheel number of bleeding-fill as actually operating by value
r.
Carry out environmental chamber to bleed-oxygenation, require O in container
2volume fraction increases to
f c-O2, calculate required-oxygenation wheel number of bleeding by formula 4
r h-O2, calculate by formula 5
rwheel bleeding-oxygenation after O in environmental chamber
2volume fraction
f pr-O2:
If calculate
r h-O2positive integer, directly with it as the bleeding of actually operating-oxygenation wheel number
r; If calculate
r h-O2being positive mark, is then the minimum positive integer larger than it-oxygenation wheel number of bleeding as actually operating by value
r.
Carry out
rwheel bleed-fill nitrogen or
rwheel bleeding-oxygenation after, N in environmental chamber
2volume fraction changes, and calculates by formula 6
rwheel is bled-fills nitrogen (or oxygen) N in environmental chamber afterwards
2volume fraction
f pr-N2:
Calculate
rwheel is bled-is filled after nitrogen in environmental chamber
f pr-N2, calculate by formula 3
f pr-O2; Calculate
rwheel bleeding-oxygenation after in environmental chamber
f pr-N2, calculate by formula 5
f pr-O2;
f pr-Vafor
rtake turns the water vapor volume fraction in environmental chamber after bleeding-inflating, its value is same
f i-Va.
2) calculating of water after gas-gas displacement-gas displacement desired gas volume components
After calculating gas-gas displacement by formula 7, water-gas replaces the gas component volumes that need add in described environmental chamber
v g':
In formula,
v gc': after gas-gas displacement, water-gas displacement need add arbitrary gas component volumes of displacement in described environmental chamber, comprises
v o2',
v cO2',
v n2',
v gx';
f pr: the volume fraction of gas-phase space gaseous fraction in gas-rear environmental chamber of gas displacement, comprises
f pr-O2,
f pr-CO2,
f pr-N2,
f pr-Va, wherein
f pr-O2calculated by formula 4 or formula 6,
f pr-CO2value 0,
f pr-Vavalue the same, all
f prsum equals 1.
3) operation steps of gas-gas displacement and water-gas displacement coupling.
1. press
v c/
v i=10, by volume
v w-isterilized water add described environmental chamber.
2. buoyancy carrier is put into described environmental chamber, culture vessel is placed on carrier, cover tightly described container cover plate.
3. when described environmental chamber is communicated with manometer, bleed in container with described hand-operating vacuum-pump, when container internal gas pressure equals 1/2 around atmospheric pressure, stop bleeding; Make described environmental chamber and N
2or O
2bladder tank is communicated with, and appropriateness extruding bladder tank, makes gas in bag enter in container, equal with around atmospheric pressure to container internal gas pressure.
4. as bled-inflated wheel number
r>=2, repeating step 3., until complete
rwheel is bled-is inflated.
5. make described environmental chamber be communicated with certain gaseous fraction bladder tank, discharge water in described environmental chamber, in described bladder tank, gas enters container, when the volume that discharges water reaches this gaseous fraction
v gc' time, stop discharging water; If now described container internal gas pressure is lower than around atmospheric pressure, the described bladder tank of appropriateness extruding, makes gas in bag continue to enter in container, equal with around atmospheric pressure to container internal gas pressure.
6. by step 5. repetitive operation, required various gaseous fraction is filled with described environmental chamber.
7. take off described gaseous fraction input module and manometer, described gaseous equilibrium bag is communicated with environmental chamber, described environmental chamber is moved in constant incubator.
8., after cell cultures carries out 30min, extract 10mL gas out from described environmental chamber, detected components concentration, the difference if any concentration of component detected value and its set(ting)value exceedes allowed band, and available gas syringe injects a small amount of component gas in container, corrects.
3. tested gas is the cell culture experiments of roatating packed bed in water
For the cell culture experiments that tested gas is roatating packed bed in water, it is all the same that the operation steps of alone water-gas displacement and coupling gas-gas replace the operation steps of replacing with water-gas, but replace formula 1 to calculate by formula 8
v gc, replace formula 7 to calculate by formula 9
v gc':
In formula 8 and formula 9,
s c: the solubleness of arbitrary gaseous fraction in water under cell culture temperature is (uptake factor a);
s i: the solubleness of arbitrary gaseous fraction in water under ambient temperature is (uptake factor a);
t 0: standard state absolute temperature (K=273).
4. the cell culture experiments under anaerobic gas phase envrionment conditions
By the coupling that described gas-gas displacement and water-gas are replaced, add water in described environmental chamber and place described buoyancy carrier and cell culture vessel, the special cartridge of described anaerobic cell culture experiments is placed in around cell culture vessel, add V-Brite B-calcium hydroxide saturated solution at cartridge, cover tightly container cover plate.Carry out 8 and take turns nitrogen of bleeding-fill, then carry out water-gas displacement, press
v gc' by CO
2and N
2be filled with environmental chamber.
The beneficial effects of the utility model are: 1. the design of buoyancy carrier and cartridge can make the residual gas phase space after placing culture vessel in environmental chamber very little, the gaseous environment that can complete most cells culture experiment with water-gas displacement is set up, also significantly reduce the burden of gas-gas displacement, the province's gas that saves time, easy and simple to handle; 2. bleed-gas replenishment process in, environmental chamber internal gas pressure change≤1/2 normal atmosphere, the range of choices of environmental chamber material used is increased, cost of manufacture reduction; 3. water-gentle-gas replacement technique of gas displacement is applied, for the quantitative input environment container of various gaseous fraction provides technical foundation simply and reliably, and by the requirement of a series of formulae discovery gaseous fraction, take into full account the factors such as the water solubility of water vapor, temperature, gas, meet the quantitative requirement of gaseous fraction in cell culture environment better; 4. use bladder tank to accommodate component gas and tested gas, kind and the source of available gas are restricted hardly; Except by commercial gas, various environmental gas and laboratory self-control gas also can be gathered with bladder tank; Prewired mixed gas can not be needed, also can without high-pressure air source, suitability is strong; 5. introduce the design of gaseous equilibrium bag, eliminate temperature variation to the impact of cell culture environment container internal gas pressure; And no matter detect from container withdrawing gas sample, or doing concentration of component toward gas injection in container corrects, and container internal gas pressure can keep stable, is also easy to operate easy.
Accompanying drawing explanation
Fig. 1 is the utility model example structure schematic diagram.
In figure, each label represents:
1. environmental chamber; 11. water service pipes; 12. water valves; 13. ventpipes; 14. ventpipes; 15. silica gel sealing rings; 16. nuts; 17. screws; 18. support bars; 2. buoyancy carrier; 21. culture plates are placed recessed; 3. cartridge; 4. gaseous fraction input module; 41. bladder tanks; 42. gas filters; 43. three-way air valves; 5. gaseous equilibrium bag; 6. vacuum elements; 61. hand-operating vacuum-pumps; 62. three-way air valves; 7. manometer; 8. Tissue Culture Plate.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail.
As shown in Figure 1, the present embodiment air ventilation device comprises environmental chamber 1, buoyancy carrier 2, cartridge 3, gaseous fraction input module 4, gaseous equilibrium bag 5, vacuum elements 6, manometer 7, Tissue Culture Plate 8.
Environmental chamber 1 be cover plate have round-ended cylinder, its material is polycarbonate (PC), water white transparency; Cylinder heights 120mm, external diameter 200mm, internal diameter 190mm, sidewall thickness 5mm; Base plate and cover plate diameter are 240mm, and thickness is 10mm; There are a circular hole in base plate central authorities, hole connect water service pipe 11, it are equipped with water valve 12; Cover plate there are ventpipe 13 and ventpipe 14; The lower panel of cover plate has a circular groove, groove external diameter is slightly larger than outside diameter of cylinder, and well width is slightly larger than cylindrical wall thickness, and groove is embedded with silica gel sealing ring 15; On base plate and cover plate, be all equally spaced 6 circular holes on the circumferential line of outer rim 10mm, and the end opening in each hole of base plate is bonded with nut 16; Screw 17 screws in the nut 16 on base plate under corresponding circular hole downwards from the circular hole cover plate, and mouth cylinder wall is embedded in cover plate circular groove, and cover plate is held on mouth cylinder.On environmental chamber 1 inwall, on the contour of nozzle circle 50mm, be equidistantly bonded with 4 support bars 18, for support buoyancy carrier 2.
Buoyancy carrier 2 is that diameter 188mm, can float over above liquid by PC plate bonding disc closed enclosure.On buoyancy carrier 2, Middle face has rectangle culture plate to place recessed 21, for placing Tissue Culture Plate 8; Culture plate places the length 4mm larger than the length of Tissue Culture Plate 8 of recessed 21, and width 40mm larger than the width of Tissue Culture Plate 8, the degree of depth is equal with the height of Tissue Culture Plate 8.
Cartridge 3 forms rectangular tight box, width 18mm by PC plate is bonding, length and height and the length of Tissue Culture Plate 8 and highly equal, for the space between filling culture plate recessed 21 sidewalls of placement and Tissue Culture Plate 8.
Gaseous fraction input module 4 is composed in series successively by bladder tank 41, gas filter 42 and three-way air valve 43, and three-way air valve 43 is connected to ventpipe 13.
Gaseous equilibrium bag 5 is medical drainage bags of 1000mL, is arranged on a port of three-way air valve 15.
Vacuum elements 6 is connected to form by hand-operating vacuum-pump 61 and three-way air valve 62, and three-way air valve 62 is connected to ventpipe 14.
Manometer 7 is precise digital display differential pressure indicators, is arranged on a port of three-way air valve 62.
Below with room temperature 20 DEG C, in environmental chamber 1
v cfor 2600mL,
v ifor 260mL, preparation 1%O
2, 5%CO
2, N
2mixed gas for Balance Air and containing saturated steam is example, and the using method of the utility model embodiment is described.
1.
r l-O2,
v cO2',
v n2' calculating
Calculate by formula 3
r l-O2:
Calculate by formula 7
v cO2':
Use formula
f c-N2=1-(
f c-O2+
f c-CO2+
f c-Va+
f c-GX) calculate
f c-N2and calculate by formula 6
f pr-N2after, calculate by formula 7
v n2':
F c-N2 =1－( 0.01 + 0.05 + 0.06 + 0 )
= 0.88
F pr-N2 = 1－(0.1+ 0.02)
= 0.88
V N2’ = (0.88 · 2600－0.88 · 260 ) · 293/310
= 1946 mL。
2. the operation steps of gas displacement.
1. get the environmental chamber 1 and connected pipeline thereof and air valve, buoyancy carrier 2, cartridge 3 and gas balance bag 5 of having made sterilising treatment, under the aseptic condition of Bechtop, assemble before carrying out the use of air ventilation device.
2. press
v ibe 260 mL, by volume
v w-isterilized water add described environmental chamber 1.
3. buoyancy carrier 2 is put into environmental chamber 1, then Tissue Culture Plate 8 is placed on buoyancy carrier 2, cover tightly container cover plate.
4. when environmental chamber 1 is communicated with manometer 7, bleed in container with described hand-operating vacuum-pump 61, when container internal gas pressure is down to 1/2 around atmospheric pressure, stop bleeding; Make environmental chamber 1 and N
2bladder tank 41 is communicated with, and appropriateness extruding bladder tank, makes N in bag
2enter in container, equal with around atmospheric pressure to container internal gas pressure.
5. open water valve 12, discharge water 1946 mL in environmental chamber 1, closes water valve 12; Appropriateness extruding N
2bladder tank, makes N in bag
2continue to enter in container, equal with around atmospheric pressure to container internal gas pressure.
6. CO is used
2bladder tank 41 replaces N
2bladder tank 41, discharge water 123 mL in environmental chamber 1, closes water valve 12; Appropriateness extruding CO
2bladder tank, makes CO in bag
2continue to enter in container, equal with around atmospheric pressure to container internal gas pressure.
7. CO is taken off from air ventilation device
2bladder tank 41, gas filter 42, hand-operating vacuum-pump 61 and manometer 7, the switch of swivel tee air valve 43, makes described gaseous equilibrium bag 5 be communicated with environmental chamber 1, is placed in constant incubator by environmental chamber 1.
8. after 30min, extract 10mL gas out from environmental chamber 1, detected components concentration, the difference if any concentration of component detected value and its set(ting)value exceedes allowed band, injects appropriate amount of components gas, corrects, proceed cell cultures with gas syringe in container.
Although the utility model discloses as above with preferred embodiment, but and be not used to limit the present invention.Any those of ordinary skill in the art, when not departing from technical solutions of the utility model scope, the technology contents of above-mentioned announcement all can be utilized to make many possible variations and modification to technical solution of the present invention, or be revised as the Equivalent embodiments of equivalent variations.Therefore, every content not departing from technical solutions of the utility model, according to the technology of the present invention essence to any simple modification made for any of the above embodiments, equivalent variations and modification, all should drop in the scope of technical solutions of the utility model protection.
Claims (4)
1. for an air ventilation device for cell cultures, it is characterized in that: this device is made up of cell culture environment container (1), buoyancy carrier (2), cartridge (3), gaseous fraction input module (4), gaseous equilibrium bag (5), vacuum elements (6), manometer (7) and connecting tube; Cell culture environment container (1) be cover plate have round-ended cylinder, on the circumferential line of nozzle 5cm, be equidistantly bonded with 4 support bars (18); Buoyancy carrier (2) is placed in cylinder, is positioned at support bar (18) top; Gaseous fraction input module (4) is connected with two ventpipes of cell culture environment container (1) cover plate respectively by three-way air valve with vacuum elements (6); Gaseous equilibrium bag (5) and manometer (7) are also connected with described two ventpipes respectively by described three-way air valve.
2. ask a kind of air ventilation device for cell cultures described in 1 according to right, it is characterized in that: the base plate of cell culture environment container (1) cylinder and the equal diameters of cover plate, be all greater than outside diameter of cylinder 2 ~ 3cm; There are a water service pipe (11) in cylinder base plate central authorities, it are equipped with water valve (12); Cover plate has ventpipe (13) and ventpipe (14), the lower panel of cover plate has a circular groove, groove is embedded with silica gel sealing ring (15); On base plate and cover plate, the circular hole that all equidistant distribution number order is equal on the circumferential line of outer rim 1 ~ 2cm, the end opening in each hole of base plate is bonded with nut (16), and screw (17) screws in the nut (16) on base plate under corresponding circular hole downwards from the circular hole cover plate, and cover plate is held on mouth cylinder.
3. according to a kind of air ventilation device for cell cultures described in claim 1, it is characterized in that: buoyancy carrier (2) is disc closed enclosure, can float on liquid, diameter is slightly less than the internal diameter of cell culture environment container (1) cylinder; It is recessed that the upper Middle face of buoyancy carrier (2) has culture vessel to place, and its recessed degree of depth is equal with the culture vessel height of placement; Cartridge (3) is placed in the space that culture vessel places between recessed sidewall and culture vessel.
4. according to a kind of air ventilation device for cell cultures described in claim 1, it is characterized in that: gaseous fraction input module (4) is composed in series by bladder tank (41), gas filter (42) and three-way air valve (43), a port of three-way air valve (43) connects the ventpipe (13) of cell culture environment container (1) cover plate, another port connects gaseous equilibrium bag (5); Vacuum elements (6) is connected to form by hand-operating vacuum-pump (61) and three-way air valve (62), three-way air valve (62) ports connect the ventpipe (14) of cell culture environment container (1) cover plate, the another port of three-way air valve (62) connect manometer (7).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400694A (en) * | 2015-12-21 | 2016-03-16 | 天津亿海生物科技有限公司 | Cell culture system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400694A (en) * | 2015-12-21 | 2016-03-16 | 天津亿海生物科技有限公司 | Cell culture system |
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