CN107557290A - A kind of perseveranceization bactogen of microalgae - Google Patents
A kind of perseveranceization bactogen of microalgae Download PDFInfo
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- CN107557290A CN107557290A CN201710902212.2A CN201710902212A CN107557290A CN 107557290 A CN107557290 A CN 107557290A CN 201710902212 A CN201710902212 A CN 201710902212A CN 107557290 A CN107557290 A CN 107557290A
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- incubator
- perseveranceization
- bactogen
- nutrient solution
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Abstract
The invention discloses a kind of perseveranceization bactogen of microalgae, it is included in both ends the first incubator and the second incubator parallel with one another, first incubator is provided with inlet and overfall, the inlet is connected with being stored with the liquid storage bottle of nutrient solution, it can be discharged after the nutrient solution filling completely the first incubator and the second incubator by overfall, second incubator is connected with air pump by airway tube, after the air pumped out through the air pump enters the second incubator, gas-liquid cycle stream is formed in the shunt circuit that the first incubator and the second incubator are formed.In the presence of gas-liquid cycle stream so that the carbon dioxide in nutrient solution and nutrient solution is sufficiently mixed with algae, and excludes the high density oxygen in container in time, on the one hand improves the utilization rate of nutrient solution, another photosynthesis for conveniently also enhancing algae.Meanwhile in the presence of gas-liquid cycle stream, the situation that effectively prevent algae adherent growth occurs.
Description
Technical field
The present invention relates to microalgae culture technique field, continuously culture fills more particularly to a kind of perseveranceization of microalgae
Put.
Background technology
Microalgae is common biology in nature, is widely used in scientific experiment.
Continuously culture is that fresh medium is consistently continuously inputted into incubator to perseveranceization, by limiting nutrition in nutrient solution
The effect of salt and the overflow of incubator maintain volume of culture constant.Continuously culture passes through limiting factor single in nutrient solution to perseveranceization
With the control of the overflow of incubator, the growth of algae in incubator is set to be in relative stability, and this stable state can be held
The continuous long period.According to the characteristics of chemostat cultivation, it is adapted to the experiment for carrying out each side such as algal grown, metabolism, toxicity.And grasp
Make and expense is relatively easy cheap, be the method for use of being worthy to be popularized.
At present on algae culture technology, no matter indoor research uses, or outdoor large-scale cultivation, has a large amount of
The research and development on its device, it is each has something to recommend him, but still following technological deficiency be present：It is because continuous culture is most of
Quiescent culture, easily cause algae adherent growth；And it is easily agglomerating, it is unfavorable for abundant mixing of the algae with nutritive salt, it is also unfavorable
In conventional algae counts.
The content of the invention
The technical problems to be solved by the invention be to provide a kind of nutrient solution it is well mixed, can effectively avoid algae adherent
Perseveranceization bactogen that is growing and being advantageous to photosynthetic microalgae.
Technical scheme used by solve above-mentioned technical problem：A kind of perseveranceization bactogen of microalgae, bag
Include and be provided with inlet and overfall in both ends the first incubator and the second incubator parallel with one another, first incubator,
The inlet is connected with being stored with the liquid storage bottle of nutrient solution, after nutrient solution filling completely first incubator and the second incubator
It can be discharged by overfall, second incubator is connected with air pump by airway tube, and the air pumped out through the air pump enters
After second incubator, gas-liquid cycle stream is formed in the shunt circuit that the first incubator and the second incubator are formed.
Further, first incubator is cylinder incubator, and second incubator is trained to be connected to cylinder
Support the helix tube type incubator below device.
Further, the both ends of the helix tube type incubator are the vertical tube that is connected with cylinder incubator, the air guide
Pipe stretches in vertical tube and downward bending make it that the mouth of pipe of airway tube is downward, and the mouth of pipe of the airway tube is horn structure.
Further, the middle part of the helix tube type incubator includes first paragraph helix tube, and the first paragraph helix tube is put
In in water vat, being contained with water in the water vat and be provided with water temperature regulater.
Further, the middle part of the helix tube type incubator includes second segment helix tube, the second segment helix tube
Some fluorescent tubes are disposed with rotating channel.
Further, Drexel bottle is provided between the air pump and airway tube, the concentrated sulfuric acid or 5% are contained with the Drexel bottle
Mercury oxide.
Further, the top of the liquid storage bottle is provided with the bottle cap with inner membrance and the inside of liquid storage bottle communicates with air.
Further, the top of the overfall, which communicates with air and has been sequentially placed from the bottom to top, blocks algae film and aseptic cotton
Ball, outlet pipe and collecting tank for collecting nutrient solution are connected between algae film and sterilized cotton ball positioned at described block.
Further, constant pressure pump is provided between the liquid storage bottle and inlet.
Further, the second segment helix tube is provided with algae solution acquisition port.
Beneficial effect：In a kind of perseveranceization bactogen of microalgae of the present invention, the first incubator and the second culture
Device both ends are in parallel, and the first incubator is provided with inlet and overfall, and inlet is connected with being stored with the liquid storage bottle of nutrient solution, training
It can be discharged after full first incubator of nutrient solution filling and the second incubator by overfall, the second incubator is connected with by airway tube
Air pump, after the air pumped out through air pump enters the second incubator, returned in the parallel connection that the first incubator and the second incubator are formed
Gas-liquid cycle stream is formed in road；In the presence of gas-liquid cycle stream so that carbon dioxide and algae in nutrient solution and nutrient solution
Class is sufficiently mixed, and excludes the high density oxygen in container in time, on the one hand improves the utilization rate of nutrient solution, another convenience
Enhance the photosynthesis of algae.Meanwhile in the presence of gas-liquid cycle stream, it effectively prevent the situation hair of algae adherent growth
It is raw.
Brief description of the drawings
The present invention is described further with reference to the accompanying drawings and examples；
Fig. 1 is a kind of schematic diagram of the perseveranceization bactogen of microalgae of the embodiment of the present invention.
Embodiment
Reference picture 1, a kind of perseveranceization bactogen of microalgae of the embodiment of the present invention, it is included in both ends mutually simultaneously
The first incubator and the second incubator of connection, specifically, the first incubator are cylinder incubator 7, and the second incubator is connection
The both ends of cylinder incubator 7 and helix tube type incubator 8 below the cylinder incubator 7, cylinder incubator 7
Left end and right-hand member above be respectively disposed with inlet 6 and overfall 13.
Inlet 6 is connected with being stored with the liquid storage bottle 2 of nutrient solution 3 by flow in pipes, and wherein the upper end of inlet 6 seals
The Filler plug 5 of quality of rubber materials is connected with, makes nutrient solution 3 and air exclusion, avoids contact with.Liquid storage bottle 2 is the translucent material of brown
Matter, both can lucifuge to a certain degree, the situation of liquid level can be observed at any time again.Also, constant pressure pump 4 is additionally provided with flow in pipes, it is adjustable
Save the speed that nutrient solution 3 is inputted in incubator；Can after nutrient solution 3 fills full cylinder incubator 7 and helix tube type incubator 8
Discharged by overfall 13.
The both ends of helix tube type incubator 8 are the vertical tube being connected with cylinder incubator 7, and left side vertical tube passes through airway tube 22
It is connected with air pump 21.Specifically, airway tube 22 is stretched in vertical tube and downward bending make it that the mouth of pipe of airway tube 22 is downward, air guide
The mouth of pipe of pipe 22 is horn structure 221；Drexel bottle 23 is provided between air pump 21 and airway tube 22, is contained with Drexel bottle 23
The concentrated sulfuric acid or 5% mercury oxide.The air that air pump 21 pumps out after the processing of the concentrated sulfuric acid or 5% mercury oxide in Drexel bottle 23,
Flowed into again by airway tube 22 in vertical tube, the end downward bending of airway tube 22 and be horn structure 221 is advantageous to air
Air pressure is formed, downward gas-liquid mixed flow flows along helix tube type incubator 8 to right-hand member, the nutrient solution 3 in cylinder incubator 7
From left end toward flowing down, followed so as to form gas-liquid in the shunt circuit that cylinder incubator 7 and helix tube type incubator 8 are formed
Circulation so that the carbon dioxide in nutrient solution 3 and nutrient solution 3 is sufficiently mixed with algae, and excludes the height in container in time
Density oxygen, on the one hand improve the utilization rate of nutrient solution 3, another photosynthesis for conveniently also enhancing algae.Meanwhile in gas-liquid
In the presence of recycle stream, the situation that effectively prevent algae adherent growth occurs.
Preferably, the middle part of helix tube type incubator 8 includes first paragraph helix tube, first paragraph helix tube is placed in water vat 11
It is interior, water is contained with water vat 11 and is provided with water temperature regulater.The temperature of nutrient solution 3 is controlled by water-bath, nutrient solution is effectively ensured
3 temperature in the production of suitable algae.
Preferably, the middle part of helix tube type incubator 8 includes second segment helix tube, the rotating channel of second segment helix tube
Inside it is disposed with some fluorescent tubes 9.The illumination of fluorescent tube 9 is distributed by spiral center, substantially increases the utilization rate of light source, and fluorescent tube 9
Intensity of illumination it is controllable, can effectively avoid the waste of light source.
Preferably, the top of liquid storage bottle 2 is provided with the bottle cap 1 with inner membrance and the inside of liquid storage bottle 2 communicates with air.Liquid storage
Bottle 2 need to can ensure that nutrient solution 3 is smoothly injected into incubator with air phase generalist, and bottle cap 1 carries inner membrance, and the aperture of inner membrance is 1.0
~2.0 μm, both can air conservation nutrient solution 3, evaporated prevented also from nutrient solution 3.
Preferably, the top of overfall 13, which communicates with air and has been sequentially placed from the bottom to top, blocks algae film 14 and sterilization
Cotton balls 12, outlet pipe 15 and collecting tank for collecting nutrient solution 3 are connected between algae film 14 and sterilized cotton ball 12 positioned at blocking
16.Sterilized cotton ball 12 after autoclave sterilization, the influence for reducing air to algae culture is served, meanwhile, block algae
Film 14 reduces the loss that algae is cultivated in 3 overflow of nutrient solution.Blocking the aperture of algae film 14 can be changed and be adjusted as needed.
Preferably, second segment helix tube is provided with algae solution acquisition port 17.Algae solution is acquired in the bottom of helix tube, grasped
Facilitate.
The early-stage preparations that algae culture is carried out using the perseveranceization bactogen of the microalgae are as follows：
1st, appropriate required nutrient solution 3 is poured into storage bottle, connects liquid storage bottle 2, constant pressure pump 4 and Filler plug 5；
2nd, the appropriate concentrated sulfuric acid of injection or 5% mercuric chloride solution in the Drexel bottle 23, by airway tube 22 by inflator pump 21,
Drexel bottle 23 and helix tube type incubator 8 are connected；
3rd, running water, liquid level submergence first paragraph helix tube 5-10cm are put in water vat 11；
4th, algae film 14 is blocked in aperture needed for being embedded in overfall 13, and sterilized cotton ball 12 is inserted into the top of overfall 13,
Overfall 13 and collecting tank 16 are connected using outlet pipe 15；
5th, intensity of light and property needed for, the fluorescent tube 9 needed for installation in second segment helix tube；
6th, the valve of algae solution acquisition port 17 is closed, ensured water-tight air tight.
The operating procedure that algae culture is carried out using the perseveranceization bactogen of the microalgae is as follows：
Filler plug 5 is stoppered, booster air pump 21 starts pump gas, the concentrated sulfuric acid or 5% chlorine that gas passes through in Drexel bottle 23
After changing mercury solution sterilizing, enter helix tube type incubator 8 through airway tube 22, and original air in device is drained；
The power supply of water vat 11 and fluorescent tube 9 is respectively turned on, regulates temperature and illumination condition.
Filler plug 5 is opened, the former algae liquid of culture is filled into incubator by inlet 6；
Filler plug 5 is stoppered, constant flow pump is opened and regulates the input rate of nutrient solution 3.
As needed, overall cycle rate can be adjusted by adjusting the power of air pump 21；Can be with incubation
When by the valve of algae solution acquisition port 17, sampled,, can be from algae solution acquisition port after the completion of culture to sample detection inspection etc.
17 all release.
By apparatus of the present invention, it can easily realize that the perseveranceization of microalgae is continuously cultivated, simple to operate, cost is cheap, can
The accuracy of experiment is greatly improved, because illumination and supply (carbon dioxide) are sufficient, and can be excluded in time highly dense in container
Oxygen is spent, advantageously in the photosynthesis of algae, improves yield.Apparatus of the present invention are related to microalgae scientific research, production etc., but not
Limitation and microalgae, some zooplankters can also be cultivated after expanding to device equal proportion, such as wheel animalcule, Daphnia magna
Contamination probability is reduced Deng, the invention, the energy and human cost is saved, there is wide utilization prospect.
Embodiments of the present invention are explained in detail above in conjunction with accompanying drawing, but the invention is not restricted to above-mentioned embodiment party
Formula, can also be before present inventive concept not be departed from the technical field those of ordinary skill possessed knowledge
Put that various changes can be made.
Claims (10)
- A kind of 1. perseveranceization bactogen of microalgae, it is characterised in that：It is included in both ends the first training parallel with one another Device and the second incubator are supported, first incubator is provided with inlet and overfall, and the inlet is with being stored with nutrient solution Liquid storage bottle is connected, and can be discharged after the nutrient solution filling completely the first incubator and the second incubator by overfall, described second Incubator is connected with air pump by airway tube, after the air pumped out through the air pump enters the second incubator, in the first incubator Gas-liquid cycle stream is formed in the shunt circuit formed with the second incubator.
- 2. the perseveranceization bactogen of microalgae according to claim 1, it is characterised in that：First incubator For cylinder incubator, second incubator is to be connected to the helix tube type incubator below cylinder incubator.
- 3. the perseveranceization bactogen of microalgae according to claim 2, it is characterised in that：The helix tube type training The both ends for supporting device are the vertical tube being connected with cylinder incubator, and the airway tube stretches in vertical tube and downward bending causes air guide The mouth of pipe of pipe is downward, and the mouth of pipe of the airway tube is horn structure.
- 4. the perseveranceization bactogen of the microalgae according to Claims 2 or 3, it is characterised in that：The helix tube The middle part of formula incubator includes first paragraph helix tube, and the first paragraph helix tube is placed in water vat, and water is contained with the water vat And it is provided with water temperature regulater.
- 5. the perseveranceization bactogen of the microalgae according to Claims 2 or 3, it is characterised in that：The helix tube The middle part of formula incubator includes second segment helix tube, and some fluorescent tubes are disposed with the rotating channel of the second segment helix tube.
- 6. the perseveranceization bactogen of the microalgae according to Claims 2 or 3, it is characterised in that：The air pump and Drexel bottle is provided between airway tube, the concentrated sulfuric acid or 5% mercury oxide are contained with the Drexel bottle.
- 7. the perseveranceization bactogen of the microalgae according to Claims 2 or 3, it is characterised in that：The liquid storage bottle Top be provided with the inside of the bottle cap with inner membrance and liquid storage bottle and communicated with air.
- 8. the perseveranceization bactogen of the microalgae according to Claims 2 or 3, it is characterised in that：The overfall Top communicate with air and be sequentially placed from the bottom to top and block algae film and sterilized cotton ball, block algae film and sterilized cotton ball positioned at described Between be connected with outlet pipe and the collecting tank for collecting nutrient solution.
- 9. the perseveranceization bactogen of the microalgae according to Claims 2 or 3, it is characterised in that：The liquid storage bottle Constant pressure pump is provided between inlet.
- 10. the perseveranceization bactogen of microalgae according to claim 5, it is characterised in that：The second segment spiral shell Coil is provided with algae solution acquisition port.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710902212.2A CN107557290B (en) | 2017-09-28 | 2017-09-28 | Continuous culture apparatus for micro algae |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710902212.2A CN107557290B (en) | 2017-09-28 | 2017-09-28 | Continuous culture apparatus for micro algae |
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| CN107557290A true CN107557290A (en) | 2018-01-09 |
| CN107557290B CN107557290B (en) | 2021-03-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710902212.2A Active CN107557290B (en) | 2017-09-28 | 2017-09-28 | Continuous culture apparatus for micro algae |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362559A (en) * | 2018-12-17 | 2019-02-22 | 郭亮 | A kind of efficiently control algae device |
| CN114431131A (en) * | 2022-01-07 | 2022-05-06 | 中国科学院东北地理与农业生态研究所 | Aeration culture method for improving survival rate of algae |
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| CN102533521A (en) * | 2010-12-22 | 2012-07-04 | 新奥科技发展有限公司 | Photo-bioreactor device |
| CN103109752A (en) * | 2013-01-05 | 2013-05-22 | 北京航空航天大学 | Experimental device of minitype creature regenerative type life support system |
| CN103756886B (en) * | 2014-01-26 | 2016-01-13 | 武汉凯迪工程技术研究总院有限公司 | A kind of method of micro-algae high-density cultured continuously and device thereof |
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2017
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Patent Citations (4)
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| CN101653106A (en) * | 2009-09-22 | 2010-02-24 | 中国水产科学研究院渔业机械仪器研究所 | Closed circulating rotifer continuous culturing system |
| CN102533521A (en) * | 2010-12-22 | 2012-07-04 | 新奥科技发展有限公司 | Photo-bioreactor device |
| CN103109752A (en) * | 2013-01-05 | 2013-05-22 | 北京航空航天大学 | Experimental device of minitype creature regenerative type life support system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362559A (en) * | 2018-12-17 | 2019-02-22 | 郭亮 | A kind of efficiently control algae device |
| CN114431131A (en) * | 2022-01-07 | 2022-05-06 | 中国科学院东北地理与农业生态研究所 | Aeration culture method for improving survival rate of algae |
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| CN107557290B (en) | 2021-03-23 |
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