CN113786379A - Lactic acid bacteria whitening and repairing essence and preparation method thereof - Google Patents
Lactic acid bacteria whitening and repairing essence and preparation method thereof Download PDFInfo
- Publication number
- CN113786379A CN113786379A CN202111187327.0A CN202111187327A CN113786379A CN 113786379 A CN113786379 A CN 113786379A CN 202111187327 A CN202111187327 A CN 202111187327A CN 113786379 A CN113786379 A CN 113786379A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus
- whitening
- parts
- skin
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000002087 whitening effect Effects 0.000 title claims abstract description 45
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims description 18
- 241000894006 Bacteria Species 0.000 title claims description 9
- 235000014655 lactic acid Nutrition 0.000 title claims description 9
- 239000004310 lactic acid Substances 0.000 title claims description 9
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 241000186660 Lactobacillus Species 0.000 claims abstract description 41
- 238000000855 fermentation Methods 0.000 claims abstract description 41
- 230000004151 fermentation Effects 0.000 claims abstract description 41
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 41
- 239000000047 product Substances 0.000 claims abstract description 37
- 239000000706 filtrate Substances 0.000 claims abstract description 22
- 239000006166 lysate Substances 0.000 claims abstract description 20
- CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000000284 extract Substances 0.000 claims abstract description 13
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 12
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 claims abstract description 12
- 235000021283 resveratrol Nutrition 0.000 claims abstract description 12
- 229940016667 resveratrol Drugs 0.000 claims abstract description 12
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 12
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 12
- 244000068988 Glycine max Species 0.000 claims abstract description 11
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 11
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000002532 grape seed extract Nutrition 0.000 claims abstract description 11
- 235000013336 milk Nutrition 0.000 claims abstract description 11
- 239000008267 milk Substances 0.000 claims abstract description 11
- 210000004080 milk Anatomy 0.000 claims abstract description 11
- 108010020346 Polyglutamic Acid Proteins 0.000 claims abstract description 10
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 9
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims abstract description 9
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 9
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 9
- 239000006046 creatine Substances 0.000 claims abstract description 9
- 229960003624 creatine Drugs 0.000 claims abstract description 9
- 229960005323 phenoxyethanol Drugs 0.000 claims abstract description 9
- 229920002643 polyglutamic acid Polymers 0.000 claims abstract description 9
- 229930003799 tocopherol Natural products 0.000 claims abstract description 9
- 229960001295 tocopherol Drugs 0.000 claims abstract description 9
- 235000010384 tocopherol Nutrition 0.000 claims abstract description 9
- 239000011732 tocopherol Substances 0.000 claims abstract description 9
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 9
- 239000000230 xanthan gum Substances 0.000 claims abstract description 9
- 229940082509 xanthan gum Drugs 0.000 claims abstract description 9
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 9
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 claims abstract description 9
- 229940087603 grape seed extract Drugs 0.000 claims abstract description 8
- 239000001717 vitis vinifera seed extract Substances 0.000 claims abstract description 8
- ANZUDYZHSVGBRF-UHFFFAOYSA-N 3-ethylnonane-1,2,3-triol Chemical compound CCCCCCC(O)(CC)C(O)CO ANZUDYZHSVGBRF-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 3
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 claims abstract 2
- 239000000203 mixture Substances 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000008367 deionised water Substances 0.000 claims description 24
- 229910021641 deionized water Inorganic materials 0.000 claims description 24
- 238000009210 therapy by ultrasound Methods 0.000 claims description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 15
- 229920002670 Fructan Polymers 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 5
- 230000001804 emulsifying effect Effects 0.000 claims description 5
- NCZPCONIKBICGS-UHFFFAOYSA-N 3-(2-ethylhexoxy)propane-1,2-diol Chemical compound CCCCC(CC)COCC(O)CO NCZPCONIKBICGS-UHFFFAOYSA-N 0.000 claims description 4
- 229940100524 ethylhexylglycerin Drugs 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 235000005205 Pinus Nutrition 0.000 claims description 2
- 241000218602 Pinus <genus> Species 0.000 claims description 2
- 238000002604 ultrasonography Methods 0.000 claims 8
- 230000000694 effects Effects 0.000 abstract description 19
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 abstract description 18
- 229920000832 Cutin Polymers 0.000 abstract description 2
- 241000235346 Schizosaccharomyces Species 0.000 abstract description 2
- 230000004888 barrier function Effects 0.000 abstract description 2
- 230000019522 cellular metabolic process Effects 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 46
- 230000000052 comparative effect Effects 0.000 description 34
- 238000012360 testing method Methods 0.000 description 30
- 239000000523 sample Substances 0.000 description 20
- 230000037394 skin elasticity Effects 0.000 description 15
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 13
- 239000002537 cosmetic Substances 0.000 description 11
- 230000001815 facial effect Effects 0.000 description 9
- 230000003020 moisturizing effect Effects 0.000 description 8
- 235000014787 Vitis vinifera Nutrition 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 5
- 230000036564 melanin content Effects 0.000 description 5
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 3
- 244000062793 Sorghum vulgare Species 0.000 description 3
- 241000219095 Vitis Species 0.000 description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 description 3
- 240000006365 Vitis vinifera Species 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 208000026935 allergic disease Diseases 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 235000019713 millet Nutrition 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 230000000529 probiotic effect Effects 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 210000001747 pupil Anatomy 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 230000037303 wrinkles Effects 0.000 description 3
- 108010051842 Advanced Oxidation Protein Products Proteins 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 244000077995 Coix lacryma jobi Species 0.000 description 2
- 235000007354 Coix lacryma jobi Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 102000003425 Tyrosinase Human genes 0.000 description 2
- 108060008724 Tyrosinase Proteins 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000003454 advanced oxidation protein product Substances 0.000 description 2
- 230000007815 allergy Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000000434 stratum corneum Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 241000186016 Bifidobacterium bifidum Species 0.000 description 1
- 102000004091 Caspase-8 Human genes 0.000 description 1
- 108090000538 Caspase-8 Proteins 0.000 description 1
- 241000113542 Codium tomentosum Species 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 241000544061 Cuculus canorus Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 208000002197 Ehlers-Danlos syndrome Diseases 0.000 description 1
- 229920002444 Exopolysaccharide Polymers 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000218231 Moraceae Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 244000153955 Reynoutria sachalinensis Species 0.000 description 1
- 235000003202 Reynoutria sachalinensis Nutrition 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 229960000271 arbutin Drugs 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000005282 brightening Methods 0.000 description 1
- 230000006315 carbonylation Effects 0.000 description 1
- 238000005810 carbonylation reaction Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000004992 fission Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- -1 flavonoid polyphenol compound Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229920000370 gamma-poly(glutamate) polymer Polymers 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 229940125721 immunosuppressive agent Drugs 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000037070 skin defense Effects 0.000 description 1
- 230000036559 skin health Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical group C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 229960001367 tartaric acid Drugs 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/347—Phenols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/88—Polyamides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9711—Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Emergency Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a lactobacillus whitening repair essence which comprises raw materials of lactobacillus/soybean milk fermentation product filtrate, lactobacillus fermentation lysate, schizosaccharomyces cerevisiae fermentation product lysate, saccharomycete/Sichuan valley seed fermentation product filtrate, trehalose, levan, tocopherol, sodium hyaluronate, a pinocembrus tomentosa extract, a grape seed extract, resveratrol, creatine, polyglutamic acid, xanthan gum, phenoxyethanol and ethylhexyl glycerol. The invention also discloses a preparation method of the lactobacillus whitening and repairing essence. The lactobacillus whitening and repairing essence disclosed by the invention can establish healthy micro-ecology of skin, strengthen the microbial barrier of the skin, simultaneously whiten the skin, soften cutin, be fine and smooth, keep moisture and be stable, promote cell metabolism, promote skin renewal and inhibit melanin activity.
Description
Technical Field
The invention relates to the field of beauty and skin care products, and particularly relates to lactic acid bacteria whitening and repairing essence and a preparation method thereof.
Background
Along with the continuous improvement of the quality of life, the market is more and more concerned about the demands of the whitening cosmetics, and various whitening cosmetics are full of the scope of the Lin Lang. At present, most of the whitening principles of the whitening cosmetics on the market aim at melanin which causes skin pigmentation and darkness, and the whitening cosmetics can reduce the generation of the melanin by inhibiting the activity of tyrosinase, or can achieve the effects of whitening and brightening the skin by carrying out catabolism and reduction on the generated melanin and blocking the generated melanin from migrating to the surface layer of the skin. The main skin whitening components in the skin care product mainly comprise vitamin C, arbutin, nicotinamide, kojic acid, tartaric acid and the like. However, skin color is often influenced by both intrinsic factors and extrinsic environmental factors, and whitening cosmetics on the market are often unsatisfactory in effect and risky in safety.
Disclosure of Invention
The invention aims to provide the probiotic whitening and repairing essence which is safe in components and can effectively whiten skin.
The invention also aims to provide a preparation method of the probiotic whitening and repairing essence.
In order to solve the problems, the invention provides a lactic acid bacteria whitening and repairing essence which comprises the following raw materials in parts by weight: 2-3 parts of lactobacillus/soybean milk fermentation product filtrate, 2-3 parts of lactobacillus fermentation lysate, 2-3 parts of schizosaccharomyces fermentation product lysate, 5-10 parts of yeast/cuckoo (COIX LACRYMA-JOBI MA-YUEN) seed fermentation product filtrate, 3-5 parts of trehalose, 2-3 parts of fructan, 1-2 parts of tocopherol (vitamin E), 0.2-0.5 part of sodium hyaluronate, 2-3 parts of CODIUM TOMENTOSUM (coumdium dinosum) extract, 2-3 parts of grape (VITIS VINIFERA) seed extract, 0.1-0.5 part of resveratrol, 0.5-1 part of creatine, 0.2-0.5 part of polyglutamic acid, 0.1-0.2 part of xanthan gum, 0.2-0.3 part of phenoxyethanol and 0.5-1 part of ethylhexylglycerin.
Preferably, the lactobacillus whitening and repairing essence further comprises 100-150 parts by weight of deionized water.
The invention also provides a method for preparing the lactobacillus whitening and repairing essence, which comprises the following steps:
(1) sequentially adding trehalose, fructan, creatine and polyglutamic acid into deionized water, uniformly dispersing, sequentially adding sodium hyaluronate and xanthan gum, and performing ultrasonic treatment under the condition of heating to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 55-60 ℃, adding tocopherol, and performing ultrasonic treatment to obtain a second mixture;
(3) adjusting the temperature of the second mixture to below 50 ℃, adding lactobacillus/soybean milk fermentation product filtrate, lactobacillus fermentation lysate, yeast/Sichuan valley seed fermentation product filtrate, pinus hirsutus extract, grape seed extract and resveratrol, and performing ultrasonic treatment to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 40-45 ℃, adding phenoxyethanol and ethylhexylglycerin, performing ultrasonic treatment, and cooling to 20-35 ℃ to obtain the lactobacillus whitening and repairing essence.
Preferably, in the step (1), the ultrasonic treatment in the heating process is carried out at a temperature of 80 to 90 ℃ and an ultrasonic frequency of 30 to 50kHz for 20 to 30 minutes.
In the step (2), the ultrasonic frequency is 30-50 kHz, and the ultrasonic time is 10-20 min;
in the step (3), the ultrasonic frequency is 40-80 kHz, and the ultrasonic time is 10-20 min.
In the step (4), the ultrasonic frequency is 40-80 kHz, and the ultrasonic time is 10-20 min.
The steps (1) to (4) are carried out in an emulsifying pot.
The lysate of fermentation product of yeast BIFIDA (BIFIDA FERMENT LYSATE, CAS 96507-89-0) is metabolite, cytoplasmic fragment, cell wall component and polysaccharide complex obtained by culturing, inactivating and decomposing Bacillus bifidus. The extraction process is similar to that of yeast fermentation product lysate, and has strong immunosuppressive activity, and can promote DNA repair and protect skin from ultraviolet ray damage. The yeast secondary fission fermentation product lysate comprises micromolecules such as vitamin B group, mineral substances, amino acids and the like which are beneficial to skin care, is high-quality yeast essence which can be used for skin care, can strengthen the metabolism of cuticle, can capture free radicals, inhibits the peroxidation of lipid, has the functions of whitening and resisting aging, is rich in nutrient substances, and has the function of nourishing the skin.
The lactobacillus fermentation lysate contains thalli and all active ingredients synthesized in the fermentation process, contains peptidoglycan, phospholipid, sterol, fatty acid, exopolysaccharide and the like, can provide rich nutrition for skin, and has good anti-aging effect.
The yeast/fructus Sichuan grain (COIX LACRYMA-JOBI MA-YUEN) seed fermentation product filtrate is mixed fermentation product filtrate of yeast and Coicis semen, and has effects of increasing skin activity, reducing wrinkle, and resisting aging; is commonly used as a skin conditioner in skin care products.
The lactobacillus/soybean milk fermentation product filtrate is black soybean milk filtrate obtained by fermenting 16 35 strains of lactobacillus, is rich in various vitamins, proteins and active ingredients, and has the effects of protecting, relieving, moisturizing, repairing and whitening.
The sodium hyaluronate is in the form of sodium salt of hyaluronic acid, is a high molecular weight linear polysaccharide, is widely distributed in animal and human connective tissue extracellular matrixes, and has good viscoelasticity, plasticity and permeability and good biocompatibility. The natural moisturizing agent is widely used in the production of cosmetics, plays a unique role as a moisturizing factor in the cosmetics, has a higher moisturizing effect compared with the traditional moisturizing agent, and has no defects of greasiness, blockage of skin pores and the like. When the sodium hyaluronate is added into cosmetics and is coated on the surface of skin, a viscoelastic transparent hydrated film can be formed, the film HAs good water retention effect, just like HA naturally existing in stem cell interstitium, the film can increase the water content of the skin stratum corneum, and the water content of the stratum corneum is closely related to the maintenance of skin health and defense function to external stimulation. The sodium hyaluronate can also permeate into the dermis to regulate the metabolism of the skin, thereby achieving the effects of wrinkle resistance, beautifying and health care.
The Arthodium hirsutum extract contains abundant vitamins and phenols, has strong moisturizing capability, and can effectively increase the moisturizing capability of skin.
Resveratrol is a non-flavonoid polyphenol compound with a stilbene structure and strong biological activity, mainly comes from plants such as grapes, giant knotweed, peanuts, mulberries and the like, has some commonness of plant polyphenol and unique biological activity. Resveratrol has obvious effects of resisting oxidation and free radicals, and has good effect on preventing and treating oxidative stress related to aging; resveratrol has good inhibitory effect on tyrosinase in B16 cells, can reduce AGEs generation amount, inhibit protein carbonylation, reduce content of Advanced Oxidation Protein Product (AOPP), and has anti-glycation effect, and also has antiinflammatory, antibacterial and moisturizing effects.
The extract of grape (VITIS VINIFERA) seed has effect in inhibiting caspase-8, which is the core component of cell apoptosis, and inhibiting it can prolong cell life, enhance skin activity, eliminate free radicals, and inhibit the production of collagenase and nitrogen oxide. In addition, at a concentration of 1%, the grape (VITIS VINIFERA) seed extract has effects in inhibiting melanin generation, shrinking and astringing pore diameter, and tightening skin.
The sodium polyglutamate has strong moisture retention capacity, can effectively increase the moisture retention capacity of skin, can resist wrinkles for a long time, and can be used as a whitening agent.
Has the advantages that: the probiotic whitening and repairing essence disclosed by the invention can establish healthy micro-ecology of skin, strengthen the microbial barrier of the skin, simultaneously whiten the skin, soften cutin, be fine and smooth, keep moisture and be stable, and can promote cell metabolism, promote skin renewal and inhibit melanin activity.
Detailed Description
Example 1
The preparation of the lactobacillus whitening and repairing essence comprises the following steps:
(1) adding 1kg of deionized water into an emulsifying pot, sequentially adding 30g of trehalose, 20g of fructan, 5g of creatine and 2g of polyglutamic acid, uniformly dispersing, sequentially adding 2g of sodium hyaluronate and 1g of xanthan gum, and carrying out ultrasonic treatment at the temperature of 80 ℃ and the ultrasonic frequency of 40kHz for 25 minutes to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 60 ℃, adding 10g of tocopherol, and carrying out ultrasonic treatment for 15 minutes at the ultrasonic frequency of 40kHz to obtain a second mixture;
(3) adjusting the temperature of the second mixture to 45 ℃, adding 30g of lactobacillus/soybean milk fermentation product filtrate, 20g of lactobacillus fermentation lysate, 20g of saccharomyces cerevisiae fermentation product lysate, 50g of saccharomycetes/Sichuan millet seed fermentation product filtrate, 20g of pinocembrus tomentosa extract, 20g of grape seed extract and 1g of resveratrol, and carrying out ultrasonic treatment for 15 minutes at the ultrasonic frequency of 60kHz to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 40 ℃, adding 2g of phenoxyethanol and 5g of ethylhexyl glycerol, carrying out ultrasonic treatment for 20 minutes at the ultrasonic frequency of 40kHz, and cooling to 25 ℃ to obtain the lactobacillus whitening and repairing essence.
Example 2
The preparation of the lactobacillus whitening and repairing essence comprises the following steps:
(1) adding 1.5kg of deionized water into an emulsifying pot, then sequentially adding 50g of trehalose, 30g of fructan, 10g of creatine and 5g of polyglutamic acid, after uniform dispersion, sequentially adding 5g of sodium hyaluronate and 2g of xanthan gum, and carrying out ultrasonic treatment at 90 ℃ and the ultrasonic frequency of 50kHz for 20 minutes to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 55 ℃, adding 20g of tocopherol, and carrying out ultrasonic treatment for 10 minutes at an ultrasonic frequency of 50kHz to obtain a second mixture;
(3) adjusting the temperature of the second mixture to 50 ℃, adding 20g of lactobacillus/soybean milk fermentation product filtrate, 30g of lactobacillus fermentation lysate, 30g of saccharomyces cerevisiae fermentation product lysate, 100g of saccharomycetes/Sichuan millet seed fermentation product filtrate, 30g of pinocembrus tomentosa extract, 30g of grape seed extract and 5g of resveratrol, and carrying out ultrasonic treatment for 10 minutes at the ultrasonic frequency of 80kHz to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 45 ℃, adding 3g of phenoxyethanol and 10g of ethylhexyl glycerol, carrying out ultrasonic treatment for 10 minutes at the ultrasonic frequency of 80kHz, and cooling to 20 ℃ to obtain the lactobacillus whitening and repairing essence.
Example 3
The preparation of the lactobacillus whitening and repairing essence comprises the following steps:
(1) adding 1.2kg of deionized water into an emulsifying pot, then sequentially adding 40g of trehalose, 25g of fructan, 7g of creatine and 3g of polyglutamic acid, after uniform dispersion, sequentially adding 3g of sodium hyaluronate and 1.5g of xanthan gum, and carrying out ultrasonic treatment at the ultrasonic frequency of 30kHz for 30 minutes at the temperature of 85 ℃ to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 55 ℃, adding 20g of tocopherol, and carrying out ultrasonic treatment for 20 minutes at the ultrasonic frequency of 30kHz to obtain a second mixture;
(3) adjusting the temperature of the second mixture to 45 ℃, adding 25g of lactobacillus/soybean milk fermentation product filtrate, 25g of lactobacillus fermentation lysate, 25g of saccharomyces cerevisiae fermentation product lysate, 70g of saccharomycetes/Sichuan millet seed fermentation product filtrate, 25g of pinocembrus tomentosa extract, 25g of grape seed extract and 3g of resveratrol, and carrying out ultrasonic treatment for 20 minutes at the ultrasonic frequency of 40kHz to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 40 ℃, adding 2.5g of phenoxyethanol and 7g of ethylhexyl glycerol, carrying out ultrasonic treatment for 15 minutes at the ultrasonic frequency of 60kHz, and cooling to 35 ℃ to obtain the lactobacillus whitening and repairing essence.
Comparative example 1
The trehalose in example 1 was replaced with deionized water, and the other conditions were unchanged to obtain the sample of comparative example 1.
Comparative example 2
The fructan of example 1 was replaced with deionized water, and the other conditions were unchanged to give the sample of comparative example 2.
Comparative example 3
The creatine in example 1 was replaced with deionized water and other conditions were unchanged to give the sample of comparative example 3.
Comparative example 4
The polyglutamic acid in example 1 was replaced with deionized water, and other conditions were unchanged to obtain a sample of comparative example 4.
Comparative example 5
The sodium hyaluronate from example 1 was replaced with deionized water and the other conditions were unchanged to obtain a sample of comparative example 5.
Comparative example 6
The xanthan gum of example 1 was replaced with deionized water and the other conditions were unchanged to give the sample of comparative example 6.
Comparative example 7
The tocopherol of example 1 was replaced with deionized water and the other conditions were unchanged to give the sample of comparative example 7.
Comparative example 8
The lactobacillus/soybean milk fermentation product filtrate of example 1 was replaced with deionized water, and the other conditions were not changed to obtain a sample of comparative example 8.
Comparative example 9
The example 1 in lactic acid bacteria fermentation lysate was replaced with deionized water, other conditions were not changed, and the sample of comparative example 9 was obtained.
Comparative example 10
The two split yeast fermentation product lysate in example 1 was replaced with deionized water, other conditions were unchanged, and the sample of comparative example 10 was obtained.
Comparative example 11
The yeast/chuangu seed fermentation product filtrate in example 1 was replaced with deionized water, and the other conditions were unchanged to obtain a sample of comparative example 11.
Comparative example 12
The Arthodium hirsutum extract of example 1 was replaced with deionized water under otherwise unchanged conditions to obtain a sample of comparative example 12.
Comparative example 13
The grape seed extract of example 1 was replaced with deionized water and the other conditions were unchanged to obtain the sample of comparative example 13.
Comparative example 14
The resveratrol from example 1 was replaced with deionized water and the other conditions were unchanged to give the sample of comparative example 14.
Comparative example 15
The phenoxyethanol of example 1 was replaced with deionized water under otherwise unchanged conditions to provide a sample of comparative example 15.
Comparative example 16
The ethylhexyl glycerin obtained in example 1 was replaced with deionized water under otherwise unchanged conditions to obtain a sample of comparative example 16.
Example 4
Randomly selecting 120 volunteers between 18-55 years old, wherein the number of the volunteers is the same as that of the volunteers, and the volunteers need to meet the following conditions:
(a) no serious systemic disease, no immunodeficiency or autoimmune disease
(b) The past has no history of allergy to skin care cosmetics;
(c) an inactive allergic disease;
(d) hormonal drugs and immunosuppressive agents have not been used systemically within one month;
(e) outdoor work is not taken as a main part, and outdoor activities exceeding the conventional amount are not performed during the test period;
(f) whitening products (including other cosmetics, external medicines or internal health care products) cannot be used 3 days before the test.
The volunteers are randomly divided into 20 groups on the premise that the number of men and women in each group is the same, 6 people in each group are selected, 5mL of the lactobacillus whitening and repairing essence prepared in the embodiment 1-3, the comparative examples 1-16 prepared in the comparative examples 1-16 and deionized water are uniformly smeared on the face every morning, noon and evening, and the test lasts for 28 days. No other skin care or cosmetic products, etc. were used during the test period.
(1) Melanin content test
Each volunteer tested the amount of melanin in the skin before the test was performed on day 1 and 2 hours after the test was performed on day 28 using a skin elasticity Cotomer MPA580 host manufactured by Courage + Khazaka, Germany, and a skin melanin Mexamer MX18 test probe manufactured by CK, Germany. The melanin content is carried out indoors, strong sunlight or direct light is avoided, the ambient temperature is 20 ℃, and the humidity is 40-60%. The volunteers were uniformly cleansed of facial skin and wiped dry with facial tissue prior to testing. The facial test area is the middle point position of the connecting line between the nose tip and the outer side of the left eye pupil.
The melanin content of each group of subjects was averaged and the results are shown in table 1.
TABLE 1 test results for melanin content
As can be seen from the data in Table 1, the skin melanin content of the volunteers using the lactobacillus whitening and repairing essence is obviously reduced, which shows that the lactobacillus whitening and repairing essence has better whitening effect.
(2) Skin elasticity test
Each volunteer tested skin elasticity before the test was performed on day 1 and 2 hours after the test was performed on day 28 using a skin elasticity Cotometer MPA580 master manufactured by Courage + Khazaka, germany, a skin elasticity PVM600 test probe manufactured by CK, germany. The skin elasticity test is carried out indoors, strong sunlight or direct light does not exist, the ambient temperature is 20 ℃, and the humidity is 40-60%. The volunteers were uniformly cleansed of facial skin and wiped dry with facial tissue prior to testing. The facial test area is the middle point position of the connecting line between the nose tip and the outer side of the left eye pupil.
Skin elasticity test instrument parameter setting: negative pressure-450 mbar, negative pressure time 2 s. The evaluation method comprises the following steps: the skin elasticity recovery parameter R2 is Ua/Uf, and the closer the R2 data is to 1, the better the skin elasticity is, the ratio of the amount of springback of the skin (Ua) in the absence of negative pressure to the maximum amount of stretch in the presence of negative pressure (Uf). The R2 data were averaged for each group of subjects and the results are shown in table 2.
Table 2 skin elastic recovery parameter test results
As can be seen from the data in Table 2, the skin elasticity recovery parameters of the volunteers using the lactobacillus whitening and repairing essence are obviously improved, which shows that the lactobacillus whitening and repairing essence has a good effect of improving the skin elasticity.
(3) Skin moisture content test
Each volunteer tested skin elasticity before the test was conducted on day 1 and 2 hours after the test was conducted on day 28 using a skin elasticity Cotomer MPA580 host manufactured by Courage + Khazaka, Germany, and a skin moisture content Cornemeter CM825 test probe manufactured by CK, Germany. The skin elasticity test is carried out indoors, strong sunlight or direct light does not exist, the ambient temperature is 20 ℃, and the humidity is 40-60%. The volunteers were uniformly cleansed of facial skin and wiped dry with facial tissue prior to testing. The facial test area is the middle point position of the connecting line between the nose tip and the outer side of the left eye pupil.
The skin Moisture content test results are expressed by set Moisture Measurement Values (MMV): the skin moisture content Cornemeter CM825 test probe is based on the principle of capacitance. When the moisture content changes, the capacitance of the skin also changes, so the moisture content on the skin surface can be analyzed by measuring the capacitance of the skin. The measured value is a relative value, and the unit is represented by C.U (Corneometer Units). The larger the MMV value, the greater the skin moisture content, and conversely, the smaller the skin moisture content.
The MMV data for each group of subjects was averaged and the results are shown in Table 3.
Table 3 skin MMV test results
As can be seen from the data in Table 3, the skin moisture content of the volunteers using the lactobacillus whitening and repairing essence is obviously improved, which shows that the lactobacillus whitening and repairing essence has a good effect of improving the skin elasticity.
(4) By reviewing the volunteers of the trial groups of the lactic acid bacterium whitening and repairing essence of example 1, example 2 and example 3, 0 person showed problems of allergy, redness and swelling or other discomfort after using the lactic acid bacterium whitening and repairing essence of the present invention.
Claims (8)
1. The lactobacillus whitening and repairing essence is characterized by comprising the following raw materials in parts by weight: 2-3 parts of lactobacillus/soybean milk fermentation product filtrate, 2-3 parts of lactobacillus fermentation lysate, 2-3 parts of yeast schizolysis product, 5-10 parts of saccharomycetes/Sichuan valley seed fermentation product filtrate, 3-5 parts of trehalose, 2-3 parts of levan, 1-2 parts of tocopherol, 0.2-0.5 part of sodium hyaluronate, 2-3 parts of pinus hirsutus extract, 2-3 parts of grape seed extract, 0.1-0.5 part of resveratrol, 0.5-1 part of creatine, 0.2-0.5 part of polyglutamic acid, 0.1-0.2 part of xanthan gum, 0.2-0.3 part of phenoxyethanol and 0.5-1 part of ethylhexylglycerol.
2. The lactic acid bacteria whitening and repairing essence according to claim 1, further comprising 100-150 parts by weight of deionized water.
3. A method for preparing the lactic acid bacteria whitening and repairing essence of any one of claims 1-2, comprising the following steps:
(1) sequentially adding trehalose, fructan, creatine and polyglutamic acid into deionized water, uniformly dispersing, sequentially adding sodium hyaluronate and xanthan gum, and performing ultrasonic treatment under the condition of heating to obtain a first mixture;
(2) adjusting the temperature of the first mixture to 55-60 ℃, adding tocopherol, and performing ultrasonic treatment to obtain a second mixture;
(3) adjusting the temperature of the second mixture to be below 50 ℃, adding lactobacillus/soybean milk fermentation product filtrate, lactobacillus fermentation lysate, yeast/Sichuan valley seed fermentation product filtrate, pinocembrus tomentosa extract, grape seed extract and resveratrol, and performing ultrasonic treatment to obtain a third mixture;
(4) and adjusting the temperature of the third mixture to 40-45 ℃, adding phenoxyethanol and ethylhexylglycerin, performing ultrasonic treatment, and cooling to 20-35 ℃ to obtain the lactobacillus whitening and repairing essence.
4. The method according to claim 3, wherein in the step (1), the ultrasound under heating is performed at a temperature of 80-90 ℃ and an ultrasound frequency of 30-50 kHz for 20-30 minutes.
5. The method according to claim 3, wherein in the step (2), the frequency of the ultrasound is 30-50 kHz, and the ultrasound time is 10-20 min.
6. The method according to claim 3, wherein in the step (3), the frequency of the ultrasound is 40-80 kHz, and the ultrasound time is 10-20 min.
7. The method according to claim 3, wherein in the step (4), the frequency of the ultrasound is 40-80 kHz, and the ultrasound time is 10-20 min.
8. The method of claim 3, wherein steps (1) - (4) are carried out in an emulsifying kettle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111187327.0A CN113786379A (en) | 2021-10-12 | 2021-10-12 | Lactic acid bacteria whitening and repairing essence and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111187327.0A CN113786379A (en) | 2021-10-12 | 2021-10-12 | Lactic acid bacteria whitening and repairing essence and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113786379A true CN113786379A (en) | 2021-12-14 |
Family
ID=78877936
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111187327.0A Pending CN113786379A (en) | 2021-10-12 | 2021-10-12 | Lactic acid bacteria whitening and repairing essence and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113786379A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114459878A (en) * | 2022-01-21 | 2022-05-10 | 东北农业大学 | Method for improving gel characteristics of fermented soybean milk by using lactobacillus casei in combination with ultrasonic treatment |
| CN115581655A (en) * | 2022-04-13 | 2023-01-10 | 广州市万千粉丝化妆品有限公司 | Application and preparation of 3X nutrient and muscle energy maintenance interstitial fluid preparation |
| CN115725475A (en) * | 2022-12-15 | 2023-03-03 | 广州悦荟化妆品有限公司 | Symbiotic fermentation schizosaccharomyces cerevisiae lysate with effects of tightening, resisting wrinkles, resisting oxidation and repairing and preparation method thereof |
| CN115887259A (en) * | 2022-11-25 | 2023-04-04 | 上海臻臣化妆品有限公司 | Anti-inflammatory and anti-aging composition, anti-inflammatory and anti-aging essence and preparation method thereof |
| CN117051048A (en) * | 2022-12-15 | 2023-11-14 | 东莞华工创为生物科技有限公司 | Lysate of fermentation product of saccharomyces cerevisiae, and preparation method and application thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586737A (en) * | 2015-01-08 | 2015-05-06 | 上海圣婕化妆品有限公司 | Probiotic whitening recovery mask essence and preparation method thereof |
| CN106399192A (en) * | 2016-10-25 | 2017-02-15 | 江苏微康生物科技有限公司 | Lactic acid bacterium fermented lysate as well as preparation method and application thereof |
| CN108338965A (en) * | 2018-04-07 | 2018-07-31 | 广州市索柔生物科技有限公司 | A kind of five cereals composition and preparation method thereof |
| CN109303716A (en) * | 2018-12-05 | 2019-02-05 | 广州蔻赛生物科技有限公司 | Plant extraction moisturizing and skin rejuvenating facial mask and preparation method thereof |
| CN110339151A (en) * | 2019-08-15 | 2019-10-18 | 广东芭薇生物科技股份有限公司 | A kind of maintenance dew and preparation method thereof |
| CN110403881A (en) * | 2019-08-29 | 2019-11-05 | 湖北省麦诗特生物科技有限公司 | A kind of preparation method and application of the river paddy seed tunning extract with white-skinned face function |
| CN111407686A (en) * | 2020-05-28 | 2020-07-14 | 上海捷丽生物科技有限公司 | Multi-effect repair spray with vegetable protein and polypeptide formula and preparation method thereof |
| CN112773761A (en) * | 2021-02-22 | 2021-05-11 | 广州花出见生物科技有限公司 | Cosmetic composition, essence and preparation method thereof |
| CN113318029A (en) * | 2021-06-17 | 2021-08-31 | 广东柏俐臣生物科技有限公司 | Moisturizing lotion and preparation method thereof |
-
2021
- 2021-10-12 CN CN202111187327.0A patent/CN113786379A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586737A (en) * | 2015-01-08 | 2015-05-06 | 上海圣婕化妆品有限公司 | Probiotic whitening recovery mask essence and preparation method thereof |
| CN106399192A (en) * | 2016-10-25 | 2017-02-15 | 江苏微康生物科技有限公司 | Lactic acid bacterium fermented lysate as well as preparation method and application thereof |
| CN108338965A (en) * | 2018-04-07 | 2018-07-31 | 广州市索柔生物科技有限公司 | A kind of five cereals composition and preparation method thereof |
| CN109303716A (en) * | 2018-12-05 | 2019-02-05 | 广州蔻赛生物科技有限公司 | Plant extraction moisturizing and skin rejuvenating facial mask and preparation method thereof |
| CN110339151A (en) * | 2019-08-15 | 2019-10-18 | 广东芭薇生物科技股份有限公司 | A kind of maintenance dew and preparation method thereof |
| CN110403881A (en) * | 2019-08-29 | 2019-11-05 | 湖北省麦诗特生物科技有限公司 | A kind of preparation method and application of the river paddy seed tunning extract with white-skinned face function |
| CN111407686A (en) * | 2020-05-28 | 2020-07-14 | 上海捷丽生物科技有限公司 | Multi-effect repair spray with vegetable protein and polypeptide formula and preparation method thereof |
| CN112773761A (en) * | 2021-02-22 | 2021-05-11 | 广州花出见生物科技有限公司 | Cosmetic composition, essence and preparation method thereof |
| CN113318029A (en) * | 2021-06-17 | 2021-08-31 | 广东柏俐臣生物科技有限公司 | Moisturizing lotion and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114459878A (en) * | 2022-01-21 | 2022-05-10 | 东北农业大学 | Method for improving gel characteristics of fermented soybean milk by using lactobacillus casei in combination with ultrasonic treatment |
| CN115581655A (en) * | 2022-04-13 | 2023-01-10 | 广州市万千粉丝化妆品有限公司 | Application and preparation of 3X nutrient and muscle energy maintenance interstitial fluid preparation |
| CN115887259A (en) * | 2022-11-25 | 2023-04-04 | 上海臻臣化妆品有限公司 | Anti-inflammatory and anti-aging composition, anti-inflammatory and anti-aging essence and preparation method thereof |
| CN115725475A (en) * | 2022-12-15 | 2023-03-03 | 广州悦荟化妆品有限公司 | Symbiotic fermentation schizosaccharomyces cerevisiae lysate with effects of tightening, resisting wrinkles, resisting oxidation and repairing and preparation method thereof |
| CN115725475B (en) * | 2022-12-15 | 2023-08-15 | 广州悦荟化妆品有限公司 | Symbiotic fermentation two-split yeast lysate with effects of tightening, resisting wrinkles, resisting oxidization and repairing and preparation method thereof |
| CN117051048A (en) * | 2022-12-15 | 2023-11-14 | 东莞华工创为生物科技有限公司 | Lysate of fermentation product of saccharomyces cerevisiae, and preparation method and application thereof |
| CN117051048B (en) * | 2022-12-15 | 2024-01-30 | 东莞华工创为生物科技有限公司 | Lysate of fermentation product of saccharomyces cerevisiae, and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113786379A (en) | Lactic acid bacteria whitening and repairing essence and preparation method thereof | |
| CN105125476B (en) | A kind of anti-wrinkle essence and preparation method thereof | |
| CN110731924A (en) | Enhanced anti-aging cosmetic compositions | |
| CN111317701B (en) | Skin barrier repair compound, skin care emulsion and preparation method thereof | |
| CN111686048B (en) | Anti-aging essence and preparation method thereof | |
| EP2859918A1 (en) | Cosmetic composition containing fermented ginseng berry pleurotus ferulae product. | |
| CN111135112A (en) | Wrinkle-removing anti-aging skin care composition and application thereof | |
| CN111939116A (en) | Anti-aging composition and preparation method thereof | |
| CN112156037A (en) | Skin-care soothing composition, makeup removing lotion and preparation method thereof | |
| CN113576975A (en) | Easily-absorbed anti-aging composition and preparation method and application thereof | |
| CN117017841A (en) | Moisturizing, skin tightening and soothing facial cream and preparation method thereof | |
| CN113456569A (en) | Skin elastic repairing and moisturizing mask and preparation method thereof | |
| CN115089533A (en) | Moisturizing and relieving essence and preparation method thereof | |
| CN112773761B (en) | Cosmetic composition, essence and preparation method thereof | |
| CN113081874A (en) | Personalized beauty treatment method based on skin | |
| CN114712285A (en) | Composition with anti-wrinkle effect and preparation method and application thereof | |
| CN114344211A (en) | Moisturizing and stabilizing composition and preparation method thereof | |
| CN106309260A (en) | Pseudo-ginseng beautifying mask | |
| CN113768853A (en) | Whitening and spot-fading composition as well as preparation method and application thereof | |
| CN113786371A (en) | Composition for preparing blueberry seaweed mask, mask and preparation method | |
| CN113384482A (en) | Moisturizing and skin-tendering essence and preparation method thereof | |
| CN111759762A (en) | Black eye removing composition, essence and preparation method thereof | |
| CN111110624A (en) | Whitening composition | |
| CN114159333B (en) | Myobase liquid and preparation method thereof | |
| CN115227620B (en) | Nourishing, moisturizing and skin brightening lotion and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211214 |
|
| RJ01 | Rejection of invention patent application after publication |