CN113620521A - Spring pond water quality treatment method - Google Patents
Spring pond water quality treatment method Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 114
- 238000000034 method Methods 0.000 title claims abstract description 30
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- 241000195493 Cryptophyta Species 0.000 claims abstract description 34
- 230000001580 bacterial effect Effects 0.000 claims abstract description 34
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 31
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 31
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 30
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 21
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 17
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 230000000813 microbial effect Effects 0.000 claims abstract description 9
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 30
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 20
- 238000004321 preservation Methods 0.000 claims description 17
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 15
- 229910052698 phosphorus Inorganic materials 0.000 claims description 15
- 239000011574 phosphorus Substances 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- 239000000645 desinfectant Substances 0.000 claims description 9
- 239000002068 microbial inoculum Substances 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 7
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 6
- 239000000460 chlorine Substances 0.000 claims description 6
- 229910052801 chlorine Inorganic materials 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 5
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 4
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 4
- 241000192700 Cyanobacteria Species 0.000 claims description 4
- 229920000153 Povidone-iodine Polymers 0.000 claims description 4
- 239000007844 bleaching agent Substances 0.000 claims description 4
- 229960001621 povidone-iodine Drugs 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000012263 liquid product Substances 0.000 claims description 3
- 238000009629 microbiological culture Methods 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 3
- YRIZYWQGELRKNT-UHFFFAOYSA-N 1,3,5-trichloro-1,3,5-triazinane-2,4,6-trione Chemical compound ClN1C(=O)N(Cl)C(=O)N(Cl)C1=O YRIZYWQGELRKNT-UHFFFAOYSA-N 0.000 claims description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 2
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 2
- 229960002233 benzalkonium bromide Drugs 0.000 claims description 2
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 claims description 2
- CEJLBZWIKQJOAT-UHFFFAOYSA-N dichloroisocyanuric acid Chemical compound ClN1C(=O)NC(=O)N(Cl)C1=O CEJLBZWIKQJOAT-UHFFFAOYSA-N 0.000 claims description 2
- 229910052740 iodine Inorganic materials 0.000 claims description 2
- 239000011630 iodine Substances 0.000 claims description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 2
- 229950009390 symclosene Drugs 0.000 claims description 2
- 102000010911 Enzyme Precursors Human genes 0.000 claims 2
- 108010062466 Enzyme Precursors Proteins 0.000 claims 2
- 238000001035 drying Methods 0.000 claims 1
- 238000005507 spraying Methods 0.000 claims 1
- 239000013505 freshwater Substances 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 239000003899 bactericide agent Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 15
- 241000238557 Decapoda Species 0.000 description 12
- 241000251468 Actinopterygii Species 0.000 description 9
- 230000000249 desinfective effect Effects 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 6
- 238000009344 polyculture Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 4
- 230000000243 photosynthetic effect Effects 0.000 description 4
- 241000195628 Chlorophyta Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000192710 Microcystis aeruginosa Species 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 241000700141 Rotifera Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 239000003124 biologic agent Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
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- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000010813 municipal solid waste Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- HDMGAZBPFLDBCX-UHFFFAOYSA-M potassium;sulfooxy sulfate Chemical compound [K+].OS(=O)(=O)OOS([O-])(=O)=O HDMGAZBPFLDBCX-UHFFFAOYSA-M 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a spring pond water quality treatment method, which comprises the steps of sunning the bottom, cleaning the pond and sterilizing, wherein microbial agents are uniformly sprayed in the whole pond after the method is sterilized, and the interval time between the sterilization and the microbial agent release is 3-5 days; the splashed microbial agent comprises one or more of zymocyte powder of bacillus subtilis MES 810, zymocyte powder of bacillus amyloliquefaciens MES812, rhodopseudomonas palustris bacterial liquid or candida utilis bacterial powder. The method uses microecological bactericide to adjust water quality, and establishes pond bacteria-algae system, which can maintain fresh water quality and good algae quality throughout the year.
Description
Technical Field
The invention belongs to the field of ecological breeding, and particularly relates to a spring pond treatment method.
Technical Field
In the early stage of cultivation, the cultivation pond usually needs to be subjected to steps of pond cleaning, disinfection, water fertilization and the like to kill wild bacteria in the pond, culture a bacterial-algae system of the pond and create a good pond environment for the growth of the cultured animals. The pond is generally subjected to a balanced benign bacteria-algae system of clearing, disinfecting and rich water culture in spring.
In the traditional pond water quality treatment in spring, the pond is generally cleaned before water is fed in to kill wild trash fish; one week later, water was added, and then sterilization was performed. After one week of disinfection, fertilizing water, and after the temperature of the fertilizing water rises, adjusting the water quality by using fungi. During the prior pond treatment, the pond is cleaned and disinfected, and then water is added, so that the establishment time of an optimal bacteria-algae system is missed.
Through continuous research and exploration of technicians of the company, an 'unconventional' method for establishing a bacteria-algae system of spring pond water quality is found. No method for establishing a bacteria-algae system by using a micro-ecological preparation is found.
Disclosure of Invention
Based on the problems, the invention aims to provide a spring pond water quality treatment method, a method for specifically adjusting water quality by using a microecological bactericide is used for establishing a pond bacteria-algae system, and fresh water quality and excellent algae quality can be maintained throughout the year. The method has high efficiency and strong pertinence, and saves energy.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a method for treating the water quality of pond in spring includes sunning the bottom of pond, cleaning pond, disinfecting, and uniformly sprinkling microbial inoculum to the whole pond after disinfection. Preferably, the interval time between the disinfection and the release of the biological agent is 3-5 days.
The splashed microbial agent comprises one or more of zymocyte powder of bacillus subtilis MES 810, zymocyte powder of bacillus amyloliquefaciens MES812, rhodopseudomonas palustris bacterial liquid or candida utilis bacterial powder.
Further, the bacterial agent source and normal dosage preferably used in the application are as follows:
(1) the number of viable bacteria in the fermented powder of Bacillus subtilis is not less than 2.0 × 1010cfu/g, normal dosage is that each kilogram of bacterial powder is used in water4-5 mu of water surface with the depth of 1 meter; the bacillus subtilis MES 810 adopted by the invention is preserved in China general microbiological culture collection center, the preservation address is No. 3 of No.1 Xilu on North Chen of the Chaozhou city, the preservation date is 8 months and 10 days in 2017, and the preservation number is CGMCC No. 14514.
(2) The number of viable bacteria in the zymocyte powder of Bacillus amyloliquefaciens MES812 is not less than 2.0 × 1010cfu/g. The normal dosage is 4-5 mu per kilogram of the bacterial powder used on the water surface with the water depth of 1 m. The bacillus amyloliquefaciens MES812 is preserved in China general microbiological culture collection management center, the preservation address is No. 3 of Xilu No.1 of Beijing republic of Chaoyang district, the preservation date is 8 months and 10 days in 2017, and the preservation number is CGMCC No. 14515;
(3) the effective viable count of the rhodopseudomonas palustris bacterial liquid product is not less than 30.0 multiplied by 108CFU/ml, the normal dosage is 0.5-1L bacterial liquid per mu of water surface with the water depth less than 2 meters, and the bacterial liquid is uniformly sprinkled by adding water.
(4) The effective viable count of the candida utilis powder product is not less than 50.0 multiplied by 108CFU/g, normal dosage is 4-5 mu per kilogram of water surface that the fungus powder can use 1 meter of water depth.
The rhodopseudomonas palustris CICC23812 used in the invention is purchased from China industrial microorganism culture preservation management center (08-21.2018), and the candida utilis CICC1314 is purchased from China industrial microorganism culture preservation management center (11-5.2018).
The bacillus subtilis and the bacillus amyloliquefaciens are subjected to shake flask culture, primary seed culture, secondary seed culture, fermentation and spray drying to prepare corresponding bacterial powder. Wherein the preparation methods of the zymocyte powder of the bacillus subtilis MES 810 and the zymocyte powder of the bacillus amyloliquefaciens MES812 are the same as the patent CN201811620757.5, namely a composite microbial agent for preventing and treating the cyanobacterial bloom in the aquaculture, and the preparation method and the application thereof.
Preferably, the type and the dosage of the microbial inoculum are further determined according to different water qualities, particularly according to the content of phosphorus and the content of ammonia nitrogen in water.
Firstly, the water quality is judged according to the content of phosphorus in the detected water body and the condition of blue algae in the water body.
When the content range of the dissolved phosphorus in the water is more than 0.5mg/L, the risk of blue algae outbreak is large, and the zymocyte powder of bacillus subtilis MES 810 and the zymocyte powder of bacillus amyloliquefaciens MES812 are applied; in this case, the amount of the aqueous dispersion is generally 4 mu per 1m per kg of the Bacillus subtilis or Bacillus amyloliquefaciens.
When the content of the dissolved phosphorus in the water is in the range of 0.1 mg/L-0.5 mg/L, if blue algae exists in the water, the fermentation bacteria powder of bacillus subtilis MES 810 and the fermentation bacteria powder of bacillus amyloliquefaciens MES812 are applied. The dosage is further determined according to the ammonia nitrogen index in the water quality.
In both cases, namely when the phosphorus content is more than 0.5mg/L and the phosphorus content is 0.1-0.5 mg/L, the zymocyte powder of bacillus subtilis MES 810 or the zymocyte powder of bacillus amyloliquefaciens MES812 need to be applied. And continuously inspecting the ammonia nitrogen condition to determine whether to increase the application amount. If the ammonia nitrogen content is less than 0.2mg/L and the transparency is less than 30cm, increasing the using amount of the zymocyte powder of the bacillus subtilis MES 810 or the zymocyte powder of the bacillus amyloliquefaciens MES 812; if the ammonia nitrogen content is 0.2-1 mg/L and the transparency is less than 30cm, increasing the using amount of the fermentation bacteria powder of the bacillus subtilis MES 810 and the fermentation bacteria powder of the bacillus amyloliquefaciens MES 812; if the ammonia nitrogen content is more than 0.2mg/L and the transparency is more than 30cm, the rhodopseudomonas palustris bacterial liquid and the candida utilis bacterial powder are added; the application amount of each bacterium after the dosage is increased is the upper limit of the normal dosage. For example, the normal use amount of the fermentation bacteria powder of bacillus subtilis MES 810 and the fermentation bacteria powder of bacillus amyloliquefaciens MES812 is 4-5 mu per kilogram of the bacteria powder used on the water surface with the water depth of 1 meter, and the use amount after the addition is 4 mu per kilogram of the bacteria powder used on the water surface with the water depth of 1 meter; the normal using amount of the rhodopseudomonas palustris bacterial liquid product is 0.5-1L of bacterial liquid used on a water surface with the water depth of less than 2 meters per mu, and the application amount is the upper limit after the application amount is increased, namely 1L of bacterial liquid is used on the water surface with the water depth of less than 2 meters per mu. Similarly, the normal usage amount of the candida utilis strain powder product is 4-5 mu per kilogram of strain powder used on the water surface with the water depth of 1m, and 4 mu per kilogram of strain powder used on the water surface with the water depth of 1m after the application amount is increased
In another case, when the content of the dissolved phosphorus in the water is in the range of 0.1 mg/L-0.5 mg/L, and when the content of ammonia nitrogen is less than 0.2mg/L and the transparency is less than 30cm under the condition that no blue-green algae exists in the water body, the zymocyte powder of bacillus subtilis MES 810 or the zymocyte powder of bacillus amyloliquefaciens MES812 is applied; and when the ammonia nitrogen content is 0.2-1 mg/L and the transparency is less than 30cm, applying the zymocyte powder of the bacillus subtilis MES 810 and the zymocyte powder of the bacillus amyloliquefaciens MES 812. When two kinds of bacteria are applied simultaneously, the dosage of each bacteria can be reduced by half, or the dosage is adjusted, but the total dosage of the bacteria in the water body accords with the range of the normal dosage. When the ammonia nitrogen content is more than 0.2mg/L and the transparency is more than 30cm, the rhodopseudomonas palustris bacterial liquid and the candida utilis bacterial powder are applied. The amount of each type of inoculum applied is the lower limit of the normal amount.
Under the condition, when the phosphorus content in the water body is less than 0.1mg/L, but blue algae exists in the water body, the ammonia nitrogen is 0.2-1 mg/L, and the transparency is more than 30cm, the zymocyte powder of bacillus subtilis MES 810 and the zymocyte powder of bacillus amyloliquefaciens MES812 are applied to cooperate with rhodopseudomonas palustris bacterial liquid and candida utilis bacterial powder. The application amount of the four microbial inoculum is determined according to the lower limit of the normal application amount.
The disinfection process and the time interval for putting in the biological agent are different according to different types of disinfectants. If the disinfectant adopted for disinfection is one of concentrated glutaraldehyde solution, benzalkonium bromide solution, povidone iodine solution and compound iodine solution or the interval time between disinfection and microbial inoculum feeding is 5 days; if the disinfectant adopted by the disinfection is a chlorine preparation, the interval time between the disinfection and the microbial inoculum is 3 days.
Preferably, the chlorine preparation is one or more of bleaching powder, strong chlorine, sodium hypochlorite, sodium dichloroisocyanurate and trichloroisocyanuric acid.
The processing method of the application is based on the following principle: the bacillus subtilis and the bacillus amyloliquefaciens have similar effects, and the combination of the bacillus subtilis and the bacillus amyloliquefaciens has better effect on preventing blue-green algae; in addition, the two kinds of algae can decompose suspended organic matters in water, decompose macromolecular nutrient substances into micromolecules for algae absorption and promote algae propagation. Aiming at the 'old water', the algae can be accelerated to split, and the water quality is changed into 'fat, alive, tender and cool'. If the dosage of the compound is large, the generation of zooplankton is accelerated, the rapid circulation of N is promoted, and the generation of nitrite in the process is reduced.
Rhodopseudomonas palustris is one kind of photosynthetic bacteria, and the strain can directly absorb sunlight to synthesize self-nutrition. The bait can degrade residual bait, excrement and other organic matters in the water body, and promotes the circulation of the organic matters by absorbing and utilizing harmful substances such as ammonia, nitrite, hydrogen sulfide and the like in the water body, thereby playing a role in improving the water quality; can promote the propagation and growth of beneficial algae, maintain algae phase balance, and has the function of fertilizing water. The photosynthetic bacteria have high nutritive value, can be used as initial feed for fish, shrimp and crab seedlings, and can improve the survival rate of seedling breeding. The Candida utilis has large cells, can be used as bait for fish, shrimp and crab seedlings, and also has the function of regulating water quality.
The invention is suitable for clearing and disinfecting a pond in spring, and is the beginning of one-year culture. The water quality in spring can be adjusted according to different water qualities, and no microbial inoculum is additionally applied in the culture process if the culture is not disinfected, so that the fresh water quality and excellent algae quality can be maintained throughout the year.
The fungi and the algae jointly form a microecosystem of the pond, the original flora system is destroyed after disinfection, and along with the decomposition and the reduction of the concentration of the disinfectant, the flora in the sediment, the air and the shoreside begin to recover and diffuse into the pond water, and the balance of the flora is achieved again. Just like the fish has more harmful bacteria in pond flora when bacterial diseases occur, the harmful bacteria are reduced after disinfection, and the newly formed flora system harmful bacteria do not reach the pathogenic quantity, so that the uninfected fish can not be infected again.
The traditional water quality treatment mode is that after disinfection, a bacteria-algae system is established in pond water, the speed is low, the quality is uncontrollable, and harmful algae such as blue algae and the like are easy to appear. The technical scheme of the invention is improved on the basis of the traditional method, and after the disinfectant is added, a large amount of bacillus or photosynthetic bacteria and saccharomycetes are artificially added at an interval of 3-5 days under the condition that the action of the disinfectant is not completely disappeared, so that a benign ecological system is formed under the mutual competitive action of the disinfection and the microbial inoculum. Although some spores (or thalli) die or delay germination due to the disinfectant, compared with a naturally formed flora system, the method has the advantages of high efficiency, controllable flora and strong pertinence. Can better culture unicellular algae to form an excellent pond bacteria-algae system. Gradually establishing the ecological balance of the pond and establishing a benign bacteria-algae system.
The excellent bacteria-algae system can promote the circulation of nitrogen in the pond, so that more energy flow enters cultured animals, the whole pond is more ecological and efficient, and the culture benefit is higher.
Compared with the prior art, the invention has the following advantages:
1. the invention fully considers the characteristics of different algae and the growth characteristics of different strains, and the scheme has strong pertinence, obvious effect and quick response;
2. the microbial agent has the characteristics of no phytotoxicity, no residue, ecology and environmental protection.
3. The invention utilizes ecological cycle to make more energy flow into the cultured animals, thereby reducing energy loss, increasing benefit and reducing environmental pollution.
Detailed Description
The invention will now be further described by way of specific examples, which are not intended to limit the scope of the invention. It will be understood by those skilled in the art that equivalent substitutions for the technical features of the present invention, or corresponding modifications, can be made within the scope of the present invention.
The instruments, reagents, materials and the like used in the following examples are, unless otherwise specified, conventional instruments, reagents, materials and the like known in the art, and are commercially available, and the experimental methods, detection methods and the like used in the following examples are, unless otherwise specified, conventional experimental methods, detection methods and the like known in the art.
Example 1 treatment of early spring Water quality in Fish and shrimp polyculture tank
(1) Time and place of experiment:
the test is carried out in two ponds of Yangzhuang Zhengcun in the quiet sea area of Tianjin, starting at 28 days 2 and 28 months 2021, the areas of the two ponds are respectively 200 mu (1#), 120 mu (2#), and the average water depth is 1.8 m.
(2) Test pond conditions and methods:
the two ponds are fish and shrimp polyculture ponds, the 1# pond is a test pond, and the 2# pond is a control pond. Before the test of 3 months and 1 day, the pond water is drained and the bottom of the pond is sunned, the two ponds are cleaned by using pond cleaning medicines respectively, and water is fed into the two ponds simultaneously in 3 months and 15 days. And 3, disinfecting the water body by using povidone iodine (the content is 10 percent, and the dosage is 100g for each mu of 1 meter water depth) for 30 days. The ammonia nitrogen content of the 1# pond detected 4 months and 5 days reaches 1.2mg/L, and the dissolved phosphorus is 0.25 mg/L. There was a small amount of blue algae in the water, and the transparency was 35 cm. Therefore, 100L of rhodopseudomonas palustris and 75kg of candida utilis are applied to the culture pond, 40kg of fermentation bacteria powder of bacillus subtilis MES 810 and 20kg of fermentation bacteria powder of bacillus amyloliquefaciens MES812 are matched. The # 2 pond did not have any treatment.
(3) And (3) test results:
when fry is released in 12 days in 4 months, the water color of the pond No.1 is fresh, the algae mainly comprises high-quality algae such as green algae, diatom, cryptophyceae and the like, and a small amount of rotifers appear; a small amount of blue algae appears in the 2# pond, and slight 'water bloom' appears when the algae grow vigorously.
Therefore, the scheme has a good function of establishing a water body bacteria-algae system in the fish and shrimp polyculture pond.
Example 2 treatment of Water quality in shrimp intensive culture ponds in early spring
(1) Time and place of experiment:
the experiment is carried out in 2 months and 28 days in 2021 in 2 open-air ponds of a green pool and a depression fishing ground in a temple of Temple of Tianjin, the 2 ponds have the areas of 20 acres (1#) and 25 acres (2#), and the average water depth of the two ponds is 2 meters.
(2) Test pond conditions and methods:
the two ponds are intensive shrimp culture ponds. The 1# pond is a test pond, and the 2# pond is a control pond; before the test is started, the pond water is drained and the bottom of the pond is sunned, the two ponds are cleaned by using pond cleaning medicines respectively, and water is fed simultaneously after one month. And 4, disinfecting the water body by using bleaching powder (25 bags for 25 mu and 20 bags for 20 mu) and disinfecting the bottom of the pool by using potassium hydrogen persulfate sheets (200 g per mu) every 12 days after 4 months. And 4, detecting that the dissolved phosphorus is not detected in a No.1 pond in 16 days at 4 months, wherein the ammonia nitrogen is 0.5mg/L, and the transparency is 32 cm.
Therefore, 10L of rhodopseudomonas palustris bacterial liquid and 5kg of candida utilis powder are applied to the 1# pond. The 2# pond was not treated at all. 50kg of "EM bacteria" (marked to contain photosynthetic bacteria, yeast and lactic acid bacteria) is sprinkled on the tea for 28 days in 4 months.
(3) And (3) test results:
in the middle ten days of the 5 th month of the pond No. 2, the water color is fresh when shrimp seedlings are put in the pond, and algae mainly comprise high-quality algae such as diatom, green algae, cryptophyceae and the like; the pond No.1 shows a small amount of blue-green algae in the middle of 5 months, and has a more and more drastic trend. Is particularly suitable for the bacillus subtilis to be sprayed and controls the development momentum of blue algae.
(4) And (4) test conclusion:
therefore, the scheme has a good function of establishing the water body bacteria-algae system in the shrimp pond.
Example 3 treatment of early spring Water quality in Fish and shrimp polyculture tank
(1) Time and place of experiment:
the test is carried out in two ponds of a new village built in the town of Wangzheng, Changzhuang in the West Qing region of Tianjin city from 28 days 2 and 28 days 2021, the areas of the two ponds are respectively 30 mu (1#), 40 mu (2#), and the average water depth is 1.8 m.
(2) Test pond conditions and methods:
the two ponds are fish and shrimp polyculture ponds, the 1# pond is a test pond, and the 2# pond is a control pond. Before the test of 28 days in 2 months, the pond water is drained and the bottom of the pond is sunned, the two ponds are cleaned by using pond cleaning medicines respectively,
two ponds started to feed water simultaneously 3 months and 10 days. Bleaching powder (25 kg per mu) is used for disinfecting water for 3 months and 20 days. Adding carp and crucian fry in 4 months and 3 days. Povidone iodine (content 10%, dosage 100g for each mu of 1m water depth) is used for disinfecting water body for 20 days in 4 months. The content of ammonia nitrogen detected by a No.1 pond in 26 days after 4 months reaches 0.3mg/L, no dissolved phosphorus is detected, and the transparency is 20cm, so that 20kg of zymocyte powder of bacillus subtilis MES 810 and 20kg of zymocyte powder of bacillus amyloliquefaciens MES812 are applied to the aquaculture pond. The # 2 pond did not have any treatment.
(3) And (3) test results:
when fry is put in 10 days in 4 months, the water color of the No.1 pond is clear, the algae mainly comprises high-quality algae such as cryptophyceae, diatom, green algae and the like, and a small amount of rotifers appear; the 2# pond has a mass propagation of algae and a dinoflagellate 'water bloom'.
Therefore, the scheme has a good function of establishing a water body bacteria-algae system in the fish and shrimp polyculture pond.
Claims (10)
1. A spring pond water quality treatment method comprises the steps of bottom drying, pond cleaning and disinfection, and is characterized in that microbial agents are uniformly sprayed in the whole pond, and the interval between disinfection and spraying of the microbial agents is 3-5 days.
2. The spring pond water quality treatment method according to claim 1, wherein the splashed microbial agent comprises one or more of zymogen powder of bacillus subtilis MES 810, zymogen powder of bacillus amyloliquefaciens MES812, rhodopseudomonas palustris bacterial liquid or candida utilis bacterial powder.
3. The method for treating water quality in a spring pond according to claim 2, wherein the number of viable bacteria in the fermentation powder of bacillus subtilis MES 810 is not less than 2.0 x 1010cfu/g, wherein the normal dosage is 4-5 mu per kilogram of the bacterial powder applied to a water surface with a water depth of 1 m; the bacillus subtilis MES 810 is preserved in China general microbiological culture collection management center, the preservation address is No. 3 of Xilu No.1 of Beijing republic of south China, the preservation date is 8 months and 10 days in 2017, and the preservation number is CGMCC No. 14514;
the number of viable bacteria in the zymocyte powder of Bacillus amyloliquefaciens MES812 is not less than 2.0 × 1010cfu/g, wherein the normal dosage is 4-5 mu per kilogram of the bacterial powder applied to a water surface with a water depth of 1 m; the Bacillus amyloliquefaciens MES812 preserved in ChinaThe general microorganism strain preservation management center has the preservation address of No. 3 Xilu No.1 of Beijing, Chaoyang, the preservation date of 8 months and 10 days in 2017 and the preservation number of CGMCC No. 14515;
the effective viable count of the rhodopseudomonas palustris bacterial liquid product is not less than 30.0 multiplied by 108cfu/ml, wherein the normal dosage is 0.5-1L of bacterial liquid used on the water surface with the water depth of less than 2 meters per mu, and the rhodopseudomonas palustris CICC23812 is purchased from China center for industrial microorganism strain preservation and management; effective viable count of not less than 50.0 in candida utilis powder product
108cfu/g, wherein the normal dosage is 4-5 mu per kilogram of the bacterial powder applied to a water surface with a water depth of 1 m; the candida utilis strain CICC1314 is purchased from China center for industrial microorganism strain preservation and management.
4. The method as claimed in claim 3, wherein the fermentation powder of Bacillus subtilis MES 810 and the fermentation powder of Bacillus amyloliquefaciens MES812 are applied when the content of dissolved phosphorus in water is more than 0.5 mg/L.
5. The method for treating water quality of a spring pond according to claim 3, wherein when the content of dissolved phosphorus in water is in the range of 0.1 mg/L-0.5 mg/L and blue algae exists in the water body, the zymocyte powder of bacillus subtilis MES 810 and the zymocyte powder of bacillus amyloliquefaciens MES812 are applied.
6. The spring pond water quality treatment method according to any one of claims 4 or 5, wherein when the ammonia nitrogen content is less than 0.2mg/L and the transparency is less than 30cm, the amount of the zymocyte powder applied with the bacillus subtilis MES 810 or the zymocyte powder applied with the bacillus amyloliquefaciens MES812 is increased; when the ammonia nitrogen content is 0.2-1 mg/L and the transparency is less than 30cm, increasing the using amount of the fermentation bacteria powder of the bacillus subtilis MES 810 and the fermentation bacteria powder of the bacillus amyloliquefaciens MES 812; when the content of ammonia nitrogen is more than 0.2mg/L and the transparency is more than 30cm, the rhodopseudomonas palustris bacterial liquid and the candida utilis bacterial powder are added and applied in a matching way; the amount administered after the increased administration is the upper limit of the normal amount.
7. The spring pond water quality treatment method according to claim 3, wherein when the content of dissolved phosphorus in the water is less than 0.5mg/L and no blue-green algae exists in the water, and when the content of ammonia nitrogen is less than 0.2mg/L and the transparency is less than 30cm, the zymocyte powder of bacillus subtilis MES 810 or the zymocyte powder of bacillus amyloliquefaciens MES812 is applied; when the ammonia nitrogen content is 0.2-1 mg/L and the transparency is less than 30cm, applying the zymocyte powder of bacillus subtilis MES 810 and the zymocyte powder of bacillus amyloliquefaciens MES 812; and when the ammonia nitrogen content is more than 0.2mg/L and the transparency is more than 30cm, applying rhodopseudomonas palustris bacterial liquid and candida utilis bacterial powder, wherein the application amount is the lower limit of the normal use amount.
8. The spring pond water quality treatment method according to claim 7, characterized in that when the phosphorus content in the water body is less than 0.1mg/L, the ammonia nitrogen content in the water body is 0.2-1 mg/L, the transparency is more than 30cm, and the water body contains blue algae, the zymocyte powder of bacillus subtilis MES 810 and the zymocyte powder of bacillus amyloliquefaciens MES812 are applied, and the rhodopseudomonas palustris bacterial liquid and the candida utilis bacterial powder are matched.
9. The spring pond water quality treatment method according to claim 1, wherein when the disinfectant used for disinfection is concentrated glutaraldehyde solution, benzalkonium bromide solution, povidone iodine solution or compound iodine solution, the interval between disinfection and microbial inoculum release is 5 days; when the disinfectant adopted by the disinfection is a chlorine preparation, the interval time between the disinfection and the microbial inoculum is 3 days.
10. The spring pond water quality treatment method according to claim 9, wherein the chlorine preparation is one or more of bleaching powder, strong chlorine, sodium hypochlorite, sodium dichloroisocyanurate and trichloroisocyanuric acid.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2376755C1 (en) * | 2008-07-23 | 2009-12-27 | ФГУП "Азовский научно-исследовательский институт рыбного хозяйства" | Method for growth of young fish of azov-chernomorskaya royal fish in ponds |
| CN104719223A (en) * | 2015-04-10 | 2015-06-24 | 宁国市金东坊农业开发有限公司 | Tor-brevifilis pond artificial aquaculture method |
| CN112723560A (en) * | 2020-12-30 | 2021-04-30 | 天津开发区坤禾生物技术有限公司 | Treatment method for water quality of northern overwintering pond |
-
2021
- 2021-08-11 CN CN202110918736.7A patent/CN113620521A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2376755C1 (en) * | 2008-07-23 | 2009-12-27 | ФГУП "Азовский научно-исследовательский институт рыбного хозяйства" | Method for growth of young fish of azov-chernomorskaya royal fish in ponds |
| CN104719223A (en) * | 2015-04-10 | 2015-06-24 | 宁国市金东坊农业开发有限公司 | Tor-brevifilis pond artificial aquaculture method |
| CN112723560A (en) * | 2020-12-30 | 2021-04-30 | 天津开发区坤禾生物技术有限公司 | Treatment method for water quality of northern overwintering pond |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116218688A (en) * | 2023-02-16 | 2023-06-06 | 天津坤禾生物科技集团股份有限公司 | Deodorization microbial agent and preparation method and application thereof |
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