CN101935627B - Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same - Google Patents
Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same Download PDFInfo
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- CN101935627B CN101935627B CN2010101992701A CN201010199270A CN101935627B CN 101935627 B CN101935627 B CN 101935627B CN 2010101992701 A CN2010101992701 A CN 2010101992701A CN 201010199270 A CN201010199270 A CN 201010199270A CN 101935627 B CN101935627 B CN 101935627B
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- DQKWXTIYGWPGOO-UHFFFAOYSA-N (2,6-dibromo-4-cyanophenyl) octanoate Chemical compound CCCCCCCC(=O)OC1=C(Br)C=C(C#N)C=C1Br DQKWXTIYGWPGOO-UHFFFAOYSA-N 0.000 title claims abstract description 70
- 241000894006 Bacteria Species 0.000 title claims abstract description 27
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 11
- 230000000593 degrading effect Effects 0.000 title claims abstract description 8
- 241000588626 Acinetobacter baumannii Species 0.000 claims abstract description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 102000016938 Catalase Human genes 0.000 claims abstract description 4
- 108010053835 Catalase Proteins 0.000 claims abstract description 4
- 102000004316 Oxidoreductases Human genes 0.000 claims abstract description 4
- 108090000854 Oxidoreductases Proteins 0.000 claims abstract description 4
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 4
- 238000003794 Gram staining Methods 0.000 claims abstract description 3
- 230000015556 catabolic process Effects 0.000 claims description 44
- 238000006731 degradation reaction Methods 0.000 claims description 44
- 239000002054 inoculum Substances 0.000 claims description 16
- 239000003905 agrochemical Substances 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- 238000000855 fermentation Methods 0.000 claims description 12
- 230000004151 fermentation Effects 0.000 claims description 12
- 238000010899 nucleation Methods 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 10
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000012856 packing Methods 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 241000726221 Gemma Species 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
- 239000012138 yeast extract Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 239000000575 pesticide Substances 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 238000012271 agricultural production Methods 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 239000002068 microbial inoculum Substances 0.000 description 5
- 239000000447 pesticide residue Substances 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 239000005489 Bromoxynil Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- -1 bromoxynil octanoates Chemical class 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000008297 liquid dosage form Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012882 sequential analysis Methods 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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Abstract
The invention provides degrading bacteria for eliminating residue of pesticide, namely bromoxynil octanoate, and a degrading bacterial agent thereof, and belongs to the technical field of biology high-technology. The strain is Gram staining reaction negative bacteria XB2 and is identified as Acinetobacter baumannii. The strain has main biological characteristics that: the strain is G-; the thallus has a short-rod shape with the size of 0.9-1.6*1.5-2.5 mu m, has no spores and is strictly aerobic; and the strain is negative for oxidase and positive for catalase and can grow by taking the bromoxynil octanoate as a unique carbon source. The degrading bacteria is directly applied to make bromoxynil octanoate residue in crops reduced by over 90 percent, solves the problem that the bromoxynil octanoate residue in agricultural production is overproof, and can produce non-toxic and nuisanceless green agricultural products.
Description
One, technical field
The present invention provides a kind of degradation bacteria of bromoxynil octanoate and the microbial inoculum of production thereof, belongs to biological high-tech field, is to utilize method of microorganism degraded chemical pesticide residual, is applicable to the production and the processing of green non-polluted farm product in the modern agriculture production.
Two, background technology
Along with agricultural planting structure and management mode change, and the change of agricultural tillage cultivating method, agricultural chemicals becomes indispensable important substance in the agricultural year stable yields gradually.Their use has promoted modern agricultural development widely; For the loss that reasons such as a large amount of due to illness Chinese caterpillar funguses do harm to cause has been retrieved in agriculture prodn; And greatly improved the efficiency and the mechanization degree of agriculture prodn, in the modernization of agriculture prodn, played immeasurable effect.Wherein chemical pesticide because of the tool saving of labor, save time, herbicidal effect is good etc., and advantage is widely used.Bromoxynil octanoate is a kind of agricultural chemicals of efficient, wide spectrum, and it absorbs through blade, in plant materials, effectively conducts, and through suppressing photosynthesis, makes plant tissue downright bad rapidly.Though; Bromoxynil octanoate has been brought into play great role in preventing and kill off farmland weed, but because its a large amount of uses have caused the serious environmental pollution problem; And through biomagnification and food chain repertory in animal and plant body even in human body, the pollution problem of agricultural chemicals today has appearred.
The enhancing of Along with people's growth in the living standard and environmental protection consciousness, the whole society also more and more pays close attention to direct harm and potential impact that pesticide residue are brought, and people press for the green agricultural product that non agricultural chemical residuum pollutes.But agricultural form and demographic situation from present China; Use to stop agricultural chemicals that to reduce farm output be impracticable as the nuisanceless production model of cost, agricultural chemicals also will continue in agriculture prodn, to bring into play enormous function as the effective means of control disease and pest.How solving this double-barreled question in the agriculture prodn just becomes the research focus of each subject.Laboratory and real application research show that all utilizing mikrobe to degrade eliminates the pesticide residue in the soil; Thereby reduce the pesticide residue in the agricultural-food such as grain, vegetables, tealeaves; The quality and the economic worth that improve agricultural-food are feasible, and to the waste water treatment of producing agricultural chemicals the certain theory foundation are provided.
Three, summary of the invention
Technical problem the objective of the invention is to practical problems and demand in the production practice; Develop out a kind of novel bacterium for degradating residual agricultural chemical; Use this microbial inoculum that the residual quantity of bromoxynil octanoate is reduced more than 90%, production and use cost are lower simultaneously.Use this degradation bacterial agent in the normal growth process of farm crop, normally to use bromoxynil octanoate to carry out disease pest and weed and prevent, and guarantee that the bromoxynil octanoate residual content meets the green food requirement in the agricultural-food.
Be main contents of the present invention below the technical scheme:
The present invention provides a kind of residual degradation bacteria of agricultural chemicals bromoxynil octanoate of eliminating; Its bacterial strain is a strain gramstaining reaction negative bacterium XB2; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on May 18th, 2010; Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.Deposit number CGMCC NO.3856 is through being accredited as Acinetobacter baumannii (Acinetobacter baumannii).Main biological characteristics is G
-, thalline is a rod-short, rod-short, no gemma, strict good supporting; Oxidase negative; Catalase is positive; Can be that sole carbon source is grown with the bromoxynil octanoate, the degradation rate that shakes bromoxynil octanoate under bottle condition in the laboratory be more than 90%.This bacterium can produce with the general fermentation equipment of fermentation industry.
The technology of using above-mentioned bromoxynil octanoate degradation bacteria to produce microbial inoculum is: inclined-plane kind-shake-flask seed liquid-seeding tank-product (the packing formulation is liquid bacterial agent).
Detailed implementation step of the present invention is:
1) the test tube kind is inoculated in the LB substratum, shaking culture is to logarithmic phase;
2) above-mentioned cultured bacterial classification is inoculated into 500 liters of seeding tanks by 10% inoculum size, be cultured to logarithmic phase, the used culture medium prescription of seeding tank is: glucose 1% (w/v, down together), (NH
4)
2SO
40.5%, K
2HPO
40.2%, MgSO
40.01%, peptone 0.05%, pH 7.2-7.5;
3) seed liquor is produced a jar cultivation by 10% inoculum size access, it is identical with the seed tank culture base to produce the used substratum of jar;
4) air flow of sterile air is 1 in the culturing process of seeding tank and production jar: 0.6-1.2; Stirring velocity is 180-240 rev/min; Culture temperature is 30-35 ℃; The whole process incubation time is 48-60 hour, after the fermentation ends thalline quantity reach 1,000,000,000/more than the ml, fermentation is accomplished the back nutrient solution and is gone out jar and directly be distributed into liquid agent with plastic barrel or packing bottle.
Beneficial effect the invention provides a kind of residual degradation bacteria of agricultural chemicals bromoxynil octanoate of eliminating, and laboratory biological degradation experimental result shows, the degradation rate of bromoxynil octanoate is reached more than 90.0%.
The bromoxynil octanoate residue degrading microbial inoculum that uses this invention to produce has advantages such as production cost is low, easy to use, removal effect is good, is adapted at national grain oil & vegetable production export base or has the local big area of green food brand mark to promote the use of.The present invention is for preserving the ecological environment, and the protection mankind's is healthy, improves additional value of farm products and has great importance.Degradation bacteria XB-2 can make the residual quantity of bromoxynil octanoate reduce more than 90%.Use this degradation bacterial agent in the normal growth process of farm crop, normally to use bromoxynil octanoate to carry out the weeds pest control and guarantee that agricultural chemicals bromoxynil octanoate residual content meets the green food requirement in the agricultural-food.And this bacterium is a Gram-negative bacteria, and is suitable in the survival time in field, agricultural chemicals result of use after can not influencing.
Field test shows, the degradation bacterial agent of producing with the present invention directly is applied to farm crop can make that agricultural chemicals bromoxynil octanoate residual quantity reduces more than 90% in the crop.The solution that the present invention is successful the residual problem that exceeds standard of agricultural chemicals bromoxynil octanoate in the agriculture prodn, both given full play to the efficiently fast effect of bromoxynil octanoate in plant pest management, can produce non-toxic and non-pollution green agricultural product again.
Four, description of drawings
Fig. 1 strain X B2 bacterium colony photo
Fig. 2 inoculum size is to the influence of strain X B2 degraded bromoxynil octanoate
The initial pH of Fig. 3 is to the influence of strain X B2 degraded bromoxynil octanoate
Fig. 4 liquid amount is to the influence of strain X B2 degraded bromoxynil octanoate
Fig. 5 initial concentration is to the influence of strain X B2 degraded bromoxynil octanoate
Fig. 6 shakes a bottle degradation experiment result
Fig. 7 field degradation experiment result
Five, embodiment
The separation of 1 bacterial strain and evaluation
Get bromoxynil octanoate enrichment bacterium liquid and carry out gradient dilution.Diluent coating contains that (prescription is: every liter contains 1.5g K on the inorganic salt solid medium of 300mg/L bromoxynil octanoate
2HPO
4, 0.5g KH
2PO
4, 0.2g MgSO
47H
2O, 1.0gNaCl, 1.0g (NH
4)
2SO
4, 15g agar, pH 7.0), cultivate a couple of days for 30 ℃.Therefrom picking list bacterium colony is verified its degradation effect, and the strain bacterial strain that degradation efficiency is higher is preserved, and carries out subsequent experimental.Its bacterium colony picture is seen Fig. 1.Through the 16SrDNA sequential analysis and combine the physio-biochemical characteristics result, be Acinetobacter baumannii with identification of strains, called after: XB2.Main biological characteristics is G
-, thalline is a rod-short, rod-short, no gemma, strict good supporting; Oxidase negative; Catalase is positive; Can be that sole carbon source is grown with the bromoxynil octanoate, the degradation rate that shakes 72 hours bromoxynil octanoates under bottle condition in the laboratory be more than 90%.This bacterium can produce with the general fermentation equipment of fermentation industry.
2 laboratory biological degradation experiments
2.1 inoculum size is to the influence of XB2 degraded bromoxynil octanoate
5) XB2 is at LB substratum (LB culture medium prescription g/L: yeast extract paste 5.00; Peptone 10.00; NaCl 10.00; PH 7.0) in be cultured to the growth logarithmic phase, the inoculum size by 1%, 3%, 5%, 10% and 15% inserts the minimal medium that contains the 100mg/L bromoxynil octanoate respectively, and (the minimal medium prescription is: every liter contains 1.5g K
2HPO
4, 0.5g KH
2PO
4, 0.2g MgSO
47H
2O, 1.0g NaCl, 1.0g (NH
4)
2SO
4, pH 7.0), Hereinafter the same) in, 30 ℃, 150r/min shaking table are cultivated.48 hours mensuration result shows, arranges from low to high by inoculum size, and the degradation rate of bromoxynil octanoate is followed successively by 38.18%, 45.31%, 56.49%, 68.34%, 80.79%, result such as Fig. 2.72 hours mensuration result shows that in the substratum of 5%, 10% and 15% inoculum size, the bromoxynil octanoate degradation rate reaches 90% basically; 1%, in the substratum of 3% inoculum size, the degradation rate of bromoxynil octanoate is respectively 70.25%, 80.60%.This shows that the size of inoculum size has direct relation to the degradation efficiency of bromoxynil octanoate, inoculum size is big more, and the degradation efficiency of bromoxynil octanoate is just high more.
2.2 initial pH is to the influence of XB2 degraded bromoxynil octanoate
XB2 is cultured to logarithmic phase in the LB substratum, be seeded in the minimal medium that contains the 100mg/L bromoxynil octanoate of different initial pH by 5%, and 30 ℃, 150r/min shaking table are cultivated, and measure the wherein content of bromoxynil octanoate behind the 72h, and the result is as shown in Figure 3.Under the condition of pH7.0-9.0, XB2 to the degradation rate of bromoxynil octanoate all greater than 75%; When pH is 7.0, degradation rate is about 90%.When pH less than 5.0 the time, XB2 is tangible downtrending to the degradation rate of bromoxynil octanoate along with the decline of pH; PH is 3.0 o'clock, and bromoxynil octanoate is not degraded basically.And under alkaline condition, bromoxynil octanoate is explained by oneself, and strain X B2 is difficult to growth under alkaline condition, and Degradation reduces.
2.3 air flow is to the influence of XB2 degraded bromoxynil octanoate
The minimal medium that in the triangular flask of 5 250mL, be respectively charged into 25,50,100,125,150mL contains the 100mg/L bromoxynil octanoate is recently indicated air flow with dress liquid percentage.XB2 cultivates logarithmic phase in the LB substratum, the inoculum size by 5% is inoculated in above-mentioned each substratum, 30 ℃, the cultivation of 150r/min shaking table, the degradation rate of mensuration bromoxynil octanoate after 72 hours, result such as Fig. 4.72 hours mensuration result shows: be equipped with 25,50,75,100,125, the degradation rate of bromoxynil octanoate is respectively 94%, 88%, 83%, 79%, 62% and 47% in the triangular flask of 150mL nutrient solution; The process of XB2 degraded bromoxynil octanoate is an aerobic processes, and big air flow helps the degraded of bromoxynil octanoate.
2.4 the initial concentration of bromoxynil octanoate is to the influence of XB2 degraded bromoxynil octanoate
XB2 is cultured to logarithmic phase in the LB substratum, be seeded in the minimal medium that contains different concns (25,50,100,200,400mg/L) bromoxynil octanoate by 5%, measures content results such as Fig. 5 of bromoxynil octanoate behind the 72h.The result shows: the interpolation of 400mg/L high density bromoxynil octanoate does not produce the obvious suppression effect to the XB2 degradation property, and 200mg/L bromoxynil octanoate degradation rate reaches 82.13%.
3 field practical application experiments
With original seed activation on petridish of the degradation bacteria XB2 of mikrobe bromoxynil octanoate pesticide residue of the present invention, and measure degradation property, be inoculated on the test tube slant subsequent use.The test tube kind is inoculated in and contains 200ml LB substratum 1000ml and shake in the bottle, and constant-temperature shaking culture is prepared the inoculation seeding tank to logarithmic phase.500 liters of seeding tanks, 400 liters of charging capacitys, culture medium prescription is: glucose 1% (w/v, down together), (NH
4)
2SO
40.5%, K
2HPO
40.2%, MgSO
40.01%, peptone 0.05%, pH 7.2-7.5.The back 121 ℃ of high pressure moist heat sterilizations that finish that feed intake, be cooled to 30 ℃ after, the above-mentioned cultured bottle bacterial classification that shakes is inserted 500 liters of seeding tanks by 10% inoculum size, be cultured to logarithmic phase, stirring velocity is 220 rev/mins, sterile air feeding amount is 1: 0.8.The seed liquor that will reach logarithmic phase is produced a jar cultivation by 10% inoculum size access, and it is identical with the seed tank culture base to produce the used medium component of jar.Produce 5 tons of tankages, 4.5 tons of charging capacitys.A back production jar 1.1kg/cm feeds intake
2Pressure under, 121 ℃ of high pressure moist heat sterilizations, below the sterilization postcooling to 30 ℃, logical sterile air keeps sterile state subsequent use.A postvaccinal production jar temperature is controlled at 30-35 ℃, and the air flow of sterile air is 1 in the culturing process of production jar: 0.8-1.0, and stirring velocity is 240 rev/mins, the whole process flow incubation time is 48-60 hour.After the fermentation ends thalline quantity reach 1,000,000,000/more than the ml.
Fermentation is accomplished the back nutrient solution and is gone out jar and directly be distributed into liquid dosage form or adopt peat to adsorb with plastic barrel or packing bottle and be distributed into the solid fungicide formulation with packing bag.
The degradation bacterial agent product is directly used and can be made in the soil bromoxynil octanoate residual quantity reduce more than 90%; Can eliminate soil pollution effectively; Alleviate plant and receive symptom of chemical damage, solved in the agriculture prodn pesticide residue problem that exceeds standard, can produce non-toxic and non-pollution green agricultural product.This degradation bacterial agent can be produced with the general fermentation equipment of fermentation industry.
1, shakes a bottle degradation experiment
In the minimal medium that contains the 100mg/L bromoxynil octanoate, the inoculum size inoculation XB2 by 5% is in 30 ℃ of shaking culture; By finding out among Fig. 6; After inoculation the 48th hour, absorption peak just began to descend, and the bromoxynil octanoate in the substratum has been degraded about 1/3; By 72 hours 95%, to 120h almost by degraded fully.
2, soil degrading experiment
It is 50mg/Kg soil that soil degrading is tested initial bromoxynil octanoate concentration, is divided into three groups: use 10
4The degradation bacteria of individual/gram soil is designated as T1, uses 10
6The degradation bacteria of individual/gram soil is designated as T2, uses 10
8The degradation bacteria of individual/gram soil is designated as T3.Experimental result shows (Fig. 7), and after 60 days, all handle the bromoxynil octanoate that all can degrade more than 80%; After 7 days, the bromoxynil octanoate degradation rate has reached 90% among the T3.Eliminate experiment through the poisoning of bromoxynil octanoate sensitive crop and find (Fig. 7), use 10
8The soil of degradation bacteria processing after 7 days of individual/gram soil is planted the sensitive crop wheat again, and the poisoning phenomenon has not taken place basically.
Claims (2)
1. agricultural chemicals bromoxynil octanoate degradation bacteria; It is characterized in that this mikrobe is the strain X B2 of Gram-negative; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on May 18th, 2010; Deposit number CGMCC NO.3856 is through being accredited as Acinetobacter baumannii (Acinetobacter baumannii); Main biological characteristics is that gramstaining is negative, and thalline is a rod-short, and no gemma is strict aerobic; Oxidase negative; Catalase is positive; Can be that sole carbon source is grown with the bromoxynil octanoate, the degradation rate that shakes bromoxynil octanoate under bottle condition in the laboratory reaches more than 90%.
2. degradation bacterial agent of producing with the described bromoxynil octanoate residue degrading of claim 1 bacterium is to produce through following method:
1) strain X B2 is inoculated in the LB substratum, shaking culture is to logarithmic phase, LB culture medium prescription g/L: yeast extract paste 5.00, and peptone 10.00, NaCl 10.00, and pH 7.0;
2) above-mentioned cultured bacterial classification is inoculated into 500 liters of seeding tanks by 10% inoculum size, be cultured to logarithmic phase, the used culture medium prescription mass volume ratio of seeding tank is: glucose 1%, (NH
4)
2SO
40.5%, K
2HPO
40.2%, MgSO
40.01%, peptone 0.05%, pH 7.2-7.5;
3) seed liquor is produced a jar cultivation by 10% inoculum size access, it is identical with the seed tank culture base to produce the used substratum of jar;
4) air flow of sterile air is 1 in the culturing process of seeding tank and production jar: 0.6-1.2; Stirring velocity is 180-240 rev/min; Culture temperature is 30-35 ℃; The whole process incubation time is 48-60 hour, after the fermentation ends thalline quantity reach 1,000,000,000/more than the ml, fermentation is accomplished the back nutrient solution and is gone out jar and directly be distributed into liquid agent with plastic barrel or packing bottle.
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| CN103243058A (en) * | 2013-05-27 | 2013-08-14 | 江苏南资环保科技有限公司 | Pseudomonas MXB-03 for degrading bromoxynil octanoate and application of pseudomonas MXB-03 |
| CN103275992A (en) * | 2013-01-24 | 2013-09-04 | 南京农业大学 | Bromo benzonitrile reduction dehalogenation enzyme gene cluster bhbA2B2 and application thereof |
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| CN102250789B (en) * | 2011-05-31 | 2012-12-19 | 黑龙江大学 | Acinetobacter baumannii capable of efficiently degrading imazamox |
| CN103243056B (en) * | 2013-05-27 | 2014-08-27 | 江苏南资环保科技有限公司 | Paracoccus MXX-04 for bromoxynil degradation and application thereof |
| CN103497918B (en) * | 2013-10-12 | 2015-04-29 | 南京农业大学 | Bromoxynil degrading bacterium and application thereof |
| CN110184219B (en) * | 2019-05-30 | 2021-06-04 | 中国石油大学(华东) | Nitrile degrading bacterium and application thereof in production of acrylic acid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584019A (en) * | 2004-06-07 | 2005-02-23 | 南京农业大学 | Moderate holophilic bacteria for degradation of phenylacetic acid and bacteria agent therefrom |
| CN100400648C (en) * | 2005-12-23 | 2008-07-09 | 南京农业大学 | Highly effective phosphorus removal bacteria and its produced bacteria formulation |
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2010
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584019A (en) * | 2004-06-07 | 2005-02-23 | 南京农业大学 | Moderate holophilic bacteria for degradation of phenylacetic acid and bacteria agent therefrom |
| CN100400648C (en) * | 2005-12-23 | 2008-07-09 | 南京农业大学 | Highly effective phosphorus removal bacteria and its produced bacteria formulation |
Non-Patent Citations (2)
| Title |
|---|
| EDWARD TOPP等.Biodegradation of the Herbicide Bromoxynil (3,5-Dibromo-4- Hydroxybenzonitrile) by Purified Pentachlorophenol Hydroxylase and Whole Cells of Flavobacterium sp. Strain ATCC 39723 Is Accompanied by Cyanogenesis.《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》.1992,第58卷(第2期),502-506. * |
| Maria S. Holtze等.Microbial degradation of the benzonitrile herbicides dichlobenil,bromoxynil and ioxynil in soil and subsurface environments e Insights into degradation pathways, persistent metabolites and involved degrader organisms.《Environmental Pollution》.2008,155-168. * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275992A (en) * | 2013-01-24 | 2013-09-04 | 南京农业大学 | Bromo benzonitrile reduction dehalogenation enzyme gene cluster bhbA2B2 and application thereof |
| CN103243058A (en) * | 2013-05-27 | 2013-08-14 | 江苏南资环保科技有限公司 | Pseudomonas MXB-03 for degrading bromoxynil octanoate and application of pseudomonas MXB-03 |
| CN103243058B (en) * | 2013-05-27 | 2014-08-27 | 江苏南资环保科技有限公司 | Pseudomonas MXB-03 for degrading bromoxynil octanoate and application of pseudomonas MXB-03 |
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| Publication number | Publication date |
|---|---|
| CN101935627A (en) | 2011-01-05 |
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