CN113583897B - Bacillus aryabhattai FL05 and application thereof - Google Patents
Bacillus aryabhattai FL05 and application thereof Download PDFInfo
- Publication number
- CN113583897B CN113583897B CN202110763592.2A CN202110763592A CN113583897B CN 113583897 B CN113583897 B CN 113583897B CN 202110763592 A CN202110763592 A CN 202110763592A CN 113583897 B CN113583897 B CN 113583897B
- Authority
- CN
- China
- Prior art keywords
- strain
- bacillus aryabhattai
- sewage treatment
- denitrification
- removal rate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/301—Aerobic and anaerobic treatment in the same reactor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a Bacillus aryabhattai FL05 strain and application thereof, wherein the Bacillus aryabhattai FL05 strain is preserved in China Center for Type Culture Collection (CCTCC) in 03-04.2021, and the preservation numbers are as follows: CCTCC NO: M2021206. The invention screens and obtains a bacterial strain FL05 with very high heterotrophic nitrification and aerobic denitrification efficiency from sludge of a sewage treatment plant, can realize efficient and synchronous nitrification and denitrification in one reactor, can be applied to sewage treatment of animal husbandry and aquaculture industry to realize efficient denitrification and remove nitrogen element pollutants in a water body. Proved by verification, the strain is opposite to COD Cr The removal rate of the bacterial strain reaches up to 76.4 percent, the removal rate of the ammonia nitrogen reaches up to 56.46 percent, and the removal rate of the nitrate nitrogen reaches up to 82.27 percent, namely the bacterial strain shows good sewage treatment capability.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a bacillus aryabhattai FL05 and application thereof.
Background
The rapid extensive development of the industry and agriculture in China causes a great amount of nitrogen-containing substances to enter natural water circulation, especially the sewage of the animal husbandry and the aquaculture industry to become one of the main sources of water pollution, so that the removal of nitrogen element pollutants in the water for sewage treatment becomes an urgent problem to be solved. The traditional biological denitrification technology is jointly completed under the aerobic nitrification of autotrophic microorganisms and the anaerobic denitrification of heterotrophic microorganisms. However, the traditional denitrification process has the problems of sectional operation, high running cost, slow reaction and the like. With the continuous and deep biological denitrification research, synchronous nitrification and denitrification microorganisms are found, and the heterotrophic nitrification-aerobic denitrification microorganisms can complete nitrification and denitrification processes in the same reactor. Therefore, the screening of a strain which can efficiently treat the aquaculture wastewater, such as the pig farm wastewater or the aquaculture wastewater, and the realization of efficient synchronous nitrification and denitrification in one reactor have important application value.
Disclosure of Invention
The invention aims to provide a Bacillus aryabhattai FL05 strain and application thereof in sewage treatment, wherein the Bacillus aryabhattai FL05 strain is separated from sludge of a sewage treatment plant, and researches show that the Bacillus aryabhattai FL05 strain has heterotrophic nitrification and aerobic denitrification effects, can finish nitrification and denitrification processes in the same reactor, and can efficiently remove dichromate, ammonia nitrogen and nitrate nitrogen in sewage when being applied to the sewage treatment, namely shows good sewage treatment capability.
One of the purposes of the invention is to provide a strain of Bacillus aryabhattai FL05 with a preservation number of: CCTCC NO: M2021206, which has been deposited in China center for type culture Collection on 03/04/2021 at the address: china, wuhan university, zip code: 430072.
further, the 16S rDNA sequence of the Bacillus aryabhattai FL05 is shown in a sequence table SEQ ID NO. 1.
Further, a culture medium for culturing the Bacillus aryabhattai FL05 is a heterotrophic nitrification culture medium or an aerobic denitrification culture medium.
Further, the heterotrophic nitrification medium comprises: 0.24g/L of ammonium sulfate, 0.82g/L of sodium citrate, 0.1g/L of potassium dihydrogen phosphate, 0.05g/L of ferrous sulfate heptahydrate, 0.2g/L of calcium chloride, 1.0g/L of magnesium sulfate heptahydrate and pH of 7.5-8.0.
Further, the aerobic denitrification culture medium comprises: 0.36g/L potassium nitrate, 0.82g/L sodium citrate, 0.1g/L potassium dihydrogen phosphate, 0.05g/L ferrous sulfate heptahydrate, 0.2g/L calcium chloride, 1.0g/L magnesium sulfate heptahydrate and pH of 6.0-6.5.
Further, the screening method of the Bacillus aryabhattai FL05 comprises the following steps: taking sludge of a sewage treatment plant, performing vortex oscillation in physiological saline, standing, taking supernatant, transferring the supernatant into a heterotrophic nitrification culture medium, performing shake culture at 30 ℃ for enrichment culture for 48 hours, performing transfer enrichment for 3 times, performing gradient dilution, coating the obtained product into a heterotrophic nitrification solid culture medium, performing culture at 30 ℃ for 72 hours, selecting a single colony, performing streak culture and purification, then inoculating the single colony into the heterotrophic nitrification or aerobic denitrification culture medium, and selecting a strain with highest heterotrophic nitrification and aerobic denitrification efficiencies to obtain the Bacillus aryabhattai FL05.
The invention also aims to provide a microbial inoculum, which comprises: the Bacillus aryabhattai strain is a fermentation broth obtained by fermenting the Bacillus aryabhattai FL05, or a dry powder microbial inoculum obtained by spray drying the fermentation broth.
The invention also aims to provide the application of the Bacillus aryabhattai FL05 strain in sewage treatment.
Further, the Bacillus aryabhattai FL05 is used for removing dichromate, ammonia nitrogen and/or nitrate nitrogen in sewage.
The fourth purpose of the invention is to provide a sewage treatment method, which comprises the following steps: inoculating the Bacillus aryabhattai FL05 strain into an LB culture medium, carrying out shake culture at 30 ℃ for 24 hours, taking a bacterial solution, centrifuging, removing a supernatant, and inoculating a bacterial precipitate into sewage for sewage treatment.
Compared with the prior art, the invention has the beneficial effects that: the invention screens a strain which has heterotrophic nitrification and aerobic denitrification effects and has the highest efficiency from sludge of a sewage treatment plant, the strain is named as Bacillus aryabhattai FL05 strain and is preserved in the sludge in 2021 in 03-04 th monthChina center for type culture Collection, the collection numbers are: CCTCC NO: M2021206. The Bacillus aryabhattai FL05 strain can realize efficient and synchronous nitrification and denitrification in one reactor, can be applied to sewage treatment in animal husbandry and aquaculture industry to realize efficient denitrification and remove nitrogen element pollutants in water, and is verified to have COD (chemical oxygen demand) by the strain Cr The removal rate of the bacterial strain reaches up to 76.4 percent, the removal rate of the ammonia nitrogen reaches up to 56.46 percent, and the removal rate of the nitrate nitrogen reaches up to 82.27 percent, namely the bacterial strain shows good sewage treatment capability.
Drawings
FIG. 1 is a phylogenetic tree constructed by sequencing the strains screened in example 1 of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1 screening and identification of heterotrophic nitrification-aerobic denitrification microorganisms
(1) Screening of strains
Heterotrophic nitrification culture medium: 0.24g/L of ammonium sulfate, 0.82g/L of sodium citrate, 0.1g/L of potassium dihydrogen phosphate, 0.05g/L of ferrous sulfate heptahydrate, 0.2g/L of calcium chloride, 1.0g/L of magnesium sulfate heptahydrate, 15g/L of agar (used for preparing a solid culture medium) and pH of 7.5-8.0.
Aerobic denitrification culture medium: ammonium sulfate is changed into 0.36g/L potassium nitrate, the pH value is 6.0-6.5, and the rest components are the same as those of the heterotrophic nitrification culture medium.
Weighing 10g of sludge in a secondary sedimentation tank of a certain municipal sewage treatment plant in Wuhan, placing the sludge in a 250mL sterile triangular flask containing 90mL sterile physiological saline and glass beads, scattering a sludge sample by vortex oscillation, standing for 1min, taking supernatant, transferring the supernatant into a heterotrophic nitrification liquid culture medium, and carrying out enrichment culture in a 120r/min shaking table at 30 ℃ for 48h.The enrichment culture was subjected to 3 times of transfer enrichment according to 10 -1 ～10 -6 After gradient dilution, the mixed solution is coated on a heterotrophic nitrification solid medium and cultured for 72 hours in a biochemical incubator at the temperature of 30 ℃. After the colonies grow out, respectively selecting 5-10 single colonies with good growth vigor, continuously performing streak culture, and continuously purifying for 2-3 times until the single colonies are determined to be pure bacteria.
Selecting pure cultured single colonies, respectively inoculating the single colonies into 100mL of heterotrophic nitrification or aerobic denitrification culture medium, carrying out shaking culture at 30 ℃ and 120r/min for 72h, measuring the concentration change of ammonia nitrogen and nitrate nitrogen in the culture medium, and preferably selecting a strain with highest heterotrophic nitrification and aerobic denitrification efficiency.
(2) 16S rDNA identification and evolutionary tree analysis of strains
The strains screened by the above method were subjected to 16S rDNA identification. Extracting the total DNA of the strain by adopting a conventional method in the field, such as a kit, performing PCR amplification, purifying a PCR product, sequencing, uploading a sequencing result to GenBank, performing homology comparison with an existing sequence in a database, and constructing a phylogenetic evolution tree by using MEGA 6.0.
The 16S rDNA sequence obtained by sequencing is shown in a sequence table SEQ ID NO 1, and the phylogenetic evolutionary tree is shown in figure 1. The identified strain is Bacillus aryabhattai, named as Bacillus aryabhattai FL05, and is preserved in China center for type culture Collection in 03 months and 04 days 2021 at the address of: china, wuhan university, zip code: 430072, with the preservation number: CCTCC NO: M2021206.
Example 2 Sewage Denitrification experiment
Carrying out pig farm wastewater denitrification experimental verification on the Bacillus aryabhattai FL05 strain, which comprises the following steps:
inoculating Bacillus aryabhattai FL05 strain into LB culture medium from a glycerol tube, culturing at 30 ℃ and 200rpm for 24h, taking 10mL of bacterial liquid, centrifuging at 8000rpm for 10min, pouring off supernatant, and using the bottom bacterial precipitate as the strain for sewage biochemical treatment experiment.
Taking sewage in a pig farm sewage collecting tank, and feeding the sewage into the pig farm sewage collecting tankAnd (4) carrying out a line-synchronous nitrification and denitrification experiment. Initial COD of pig farm wastewater Cr The concentration is about 4600mg/L, the ammonia nitrogen concentration is about 830mg/L, and the nitrate nitrogen concentration is about 280mg/L. Diluting the pig farm wastewater by 5 times, 10 times and 20 times respectively, taking 1L of the diluted pig farm wastewater, placing the diluted pig farm wastewater in a 2L beaker, inoculating the centrifuged FL05 thallus precipitate, monitoring the dissolved oxygen in the water body by using an oxygen dissolving electrode, and keeping the dissolved oxygen in the water body at about 2mg/L by controlling the aeration intensity. Samples were taken at 24h,48h, and 72h post-inoculation, respectively, to determine COD in each experimental group Cr And the concentrations of ammonia nitrogen and nitrate nitrogen, and calculating the removal rate after 72 hours of treatment, wherein the experimental result is shown in table 1.
TABLE 1 Sewage treatment Effect of FL05 Strain
The results show that the FL05 strain has COD in the wastewater over a prolonged treatment period Cr The removal rate of ammonia nitrogen and nitrate nitrogen is gradually improved, and after 72 hours of treatment, the FL05 strain can be used for treating COD Cr Removal rates of (1) were 76.37% (5 ×), 77.41% (10 ×) and 71.33% (20 ×), respectively; the removal rate of ammonia nitrogen is 19.88% (5X), 41.70% (10X) and 56.46% (20X); the removal rates for nitrate nitrogen were 71.58% (5 ×), 80.21% (10 ×) and 82.27% (20 ×). The result shows that the Bacillus aryabhattai FL05 strain has good removal rate on three pollutants in sewage under the condition of not domesticating, namely the strain has good sewage treatment capability.
While the invention has been described with reference to specific preferred embodiments, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the following claims.
Sequence listing
<110> university of agriculture in Huazhong
<120> bacillus aryabhattai FL05 and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1458
<212> DNA
<213> Bacillus aryabhattai FL05 (Bacillus aryabhattai FL 05)
<400> 1
gagtgctgcg cgtgctatac atgcagtcga gcgaactgat tagaagcttg cttctatgac 60
gttagcggcg gacgggtgag taacacgtgg gcaacctgcc tgtaagactg ggataacttc 120
gggaaaccga agctaatacc ggataggatc ttctccttca tgggagatga ttgaaagatg 180
gtttcggcta tcacttacag atgggcccgc ggtgcattag ctagttggtg aggtaacggc 240
tcaccaaggc aacgatgcat agccgacctg agagggtgat cggccacact gggactgaga 300
cacggcccag actcctacgg gaggcagcag tagggaatct tccgcaatgg acgaaagtct 360
gacggagcaa cgccgcgtga gtgatgaagg ctttcgggtc gtaaaactct gttgttaggg 420
aagaacaagt acgagagtaa ctgctcgtac cttgacggta cctaaccaga aagccacggc 480
taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttatccg gaattattgg 540
gcgtaaagcg cgcgcaggcg gtttcttaag tctgatgtga aagcccacgg ctcaaccgtg 600
gagggtcatt ggaaactggg gaacttgagt gcagaagaga aaagcggaat tccacggtgt 660
agcggtgaaa tgcgtagaga tgtggaggaa caccaggtgg cgaaggcggc tttttggtct 720
gtaactgacg ctgaggcgcg aaagcgtggg gagcaaacag gattagatac cctggtagtc 780
cacgccgtaa acgatgagtg ctaagtgtta gagggtttcc gccctttagt gctgcagcta 840
acgcattaag cactccgcct ggggagtacg gtcgcaagac tgaaactcaa aggaattgac 900
gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac 960
caggtcttga catcctctga caactctaga gatagagcgt tccccttcgg gggacagagt 1020
gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa 1080
cgagcgcaac ccttgatctt agttgccagc atttagttgg gcactctaag gtgactgccg 1140
gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgacctgggc 1200
tacacacgtg ctacaatgga tggtacaaag ggctgcaaga ccgcgaggtc aagccaatcc 1260
cataaaacca ttctcagttc ggattgtagg ctgcaactcg cctacatgaa gctggaatcg 1320
ctagtaatcg cggatcagca tgccgcggtg aatacgttcc cgggccttgt acacaccgcc 1380
cgtcacacca cgagagtttg taacacccga agtcggtgga gtaaccgtaa ggagctagcc 1440
gcctaaggga cagaagtg 1458
Claims (4)
1. A strain of Bacillus aryabhattai (Bacillus aryabhattai) FL05 is characterized in that the preservation number of the Bacillus aryabhattai FL05 is as follows: CCTCC NO: M2021206.
2. The use of the Bacillus aryabhattai (Bacillus aryabhattai) FL05 strain of claim 1 in sewage treatment.
3. Use according to claim 2, characterized in that the Bacillus aryabhattai (Bacillus aryabhattai) FL05 is used for removing COD Cr, ammonia nitrogen and/or nitrate nitrogen from sewage.
4. A method of treating wastewater, the method comprising: the Bacillus aryabhattai (Bacillus aryabhattai) FL05 strain of claim 1 is inoculated in an LB culture medium, shake-cultured at 30 ℃ for 24 hours, the strain liquid is taken out, centrifuged, the supernatant is discarded, and the strain precipitate is inoculated in sewage for sewage treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110763592.2A CN113583897B (en) | 2021-07-06 | 2021-07-06 | Bacillus aryabhattai FL05 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110763592.2A CN113583897B (en) | 2021-07-06 | 2021-07-06 | Bacillus aryabhattai FL05 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113583897A CN113583897A (en) | 2021-11-02 |
| CN113583897B true CN113583897B (en) | 2023-04-18 |
Family
ID=78246161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110763592.2A Active CN113583897B (en) | 2021-07-06 | 2021-07-06 | Bacillus aryabhattai FL05 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113583897B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114031189B (en) * | 2021-11-29 | 2022-11-22 | 江南大学 | Method for treating sewage with low carbon-nitrogen ratio |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603907B (en) * | 2017-09-27 | 2020-10-16 | 北京农学院 | Bacillus aryabhattai GH-9 and application thereof |
| CN109706102B (en) * | 2019-03-04 | 2020-11-20 | 河北萌帮水溶肥料股份有限公司 | Bacillus aryabhattai with silicon-decomposing function and application thereof |
-
2021
- 2021-07-06 CN CN202110763592.2A patent/CN113583897B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113583897A (en) | 2021-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105733998B (en) | Efficient denitrification strain with heterotrophic nitrification and aerobic denitrification capabilities | |
| CN114890555B (en) | Solid microbial preparation for treating rural black and odorous water body and preparation method and application thereof | |
| CN107460141B (en) | Heterotrophic nitrification aerobic denitrification citrobacter and application thereof | |
| CN108342339B (en) | Klebsiella strain and application thereof in river sewage and rural ammonia nitrogen-containing domestic sewage | |
| CN111117914B (en) | Salt-tolerant heterotrophic aerobic nitrobacteria strain, culture method, bacterial liquid and application | |
| CN114703095B (en) | Pseudomonas adulthood and application thereof in field of sewage and wastewater purification | |
| CN110656066B (en) | Acinetobacter strain for shortcut nitrification and denitrification and application thereof | |
| CN109337832A (en) | A kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application | |
| CN113234626A (en) | Strain with heterotrophic nitrification-aerobic denitrification function and application thereof | |
| CN101811779B (en) | Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same | |
| CN114940957B (en) | Paracoccus ubitus with facultative denitrification synchronous denitrification and dephosphorization performances | |
| CN113583897B (en) | Bacillus aryabhattai FL05 and application thereof | |
| CN110184217B (en) | Salt-tolerant denitrifying bacterium taking nitrite as nitrogen source and application thereof | |
| CN114292798B (en) | Anaerobic denitrifying strain and application thereof in riverway water body remediation | |
| CN108102943B (en) | Denitrifying microorganism and application thereof | |
| CN113699060B (en) | Low-carbon high-nitrogen-resistant heterotrophic nitrification-aerobic denitrification sphingosine box fungus and application thereof | |
| CN116836847A (en) | Heterotrophic nitrification-aerobic denitrification bacteria and application thereof | |
| CN113416681B (en) | Heterotrophic nitrification-aerobic denitrification bacteria resistant to low carbon and high nitrogen and application thereof | |
| CN110184223B (en) | Arthrobacter calsium capable of removing ammonia nitrogen in culture sewage and application thereof | |
| CN115747096A (en) | Sphingosine strain and application thereof in river water body treatment | |
| CN110468066B (en) | Aerobic denitrifying strain and application thereof | |
| CN115353210A (en) | Application of bacillus pumilus LZP02 in treatment of pig raising wastewater | |
| CN101811780A (en) | Preparation method and application of halophilic decontamination bacterial agent | |
| CN115975845B (en) | Application of salt-resistant/acid-resistant heterotrophic nitrification-aerobic denitrification bacteria in environmental wastewater treatment | |
| CN117384791B (en) | Pseudomonas for promoting anaerobic iron ammoxidation process and application thereof in sewage treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |