CN113455470B - Method for artificially culturing toad feeding insects - Google Patents
Method for artificially culturing toad feeding insects Download PDFInfo
- Publication number
- CN113455470B CN113455470B CN202110992506.5A CN202110992506A CN113455470B CN 113455470 B CN113455470 B CN 113455470B CN 202110992506 A CN202110992506 A CN 202110992506A CN 113455470 B CN113455470 B CN 113455470B
- Authority
- CN
- China
- Prior art keywords
- maggot
- toad
- chamber
- toads
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000238631 Hexapoda Species 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000012258 culturing Methods 0.000 title claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 52
- 241000269420 Bufonidae Species 0.000 claims abstract description 51
- 241000202815 Chrysomya megacephala Species 0.000 claims abstract description 39
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229940000207 selenious acid Drugs 0.000 claims abstract description 33
- MCAHWIHFGHIESP-UHFFFAOYSA-N selenous acid Chemical compound O[Se](O)=O MCAHWIHFGHIESP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000000203 mixture Substances 0.000 claims abstract description 30
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000004005 microsphere Substances 0.000 claims abstract description 20
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 claims abstract description 19
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000004537 pulping Methods 0.000 claims abstract description 18
- 238000004140 cleaning Methods 0.000 claims abstract description 14
- 235000012907 honey Nutrition 0.000 claims abstract description 11
- 244000183685 Citrus aurantium Species 0.000 claims abstract description 6
- 235000007716 Citrus aurantium Nutrition 0.000 claims abstract description 6
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims abstract description 6
- 239000010446 mirabilite Substances 0.000 claims abstract description 6
- 239000002002 slurry Substances 0.000 claims description 24
- 238000009395 breeding Methods 0.000 claims description 19
- 230000001488 breeding effect Effects 0.000 claims description 17
- 239000011148 porous material Substances 0.000 claims description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000011521 glass Substances 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 10
- 238000010009 beating Methods 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 9
- 238000003760 magnetic stirring Methods 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 7
- 238000009423 ventilation Methods 0.000 claims description 7
- 208000002173 dizziness Diseases 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 241001673966 Magnolia officinalis Species 0.000 claims description 5
- 235000009411 Rheum rhabarbarum Nutrition 0.000 claims description 5
- 235000019486 Sunflower oil Nutrition 0.000 claims description 5
- 230000032050 esterification Effects 0.000 claims description 5
- 238000005886 esterification reaction Methods 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000002600 sunflower oil Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 244000299790 Rheum rhabarbarum Species 0.000 claims 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 abstract description 7
- 229910052711 selenium Inorganic materials 0.000 abstract description 7
- 239000011669 selenium Substances 0.000 abstract description 7
- 239000004480 active ingredient Substances 0.000 abstract description 4
- 201000011510 cancer Diseases 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 240000004980 Rheum officinale Species 0.000 abstract 1
- 235000008081 Rheum officinale Nutrition 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 14
- 241000269417 Bufo Species 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 6
- 229940091258 selenium supplement Drugs 0.000 description 6
- 239000003088 amphibian venom Substances 0.000 description 5
- 238000012364 cultivation method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000219061 Rheum Species 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000001678 irradiating effect Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 102000008114 Selenoproteins Human genes 0.000 description 2
- 108010074686 Selenoproteins Proteins 0.000 description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 230000007969 cellular immunity Effects 0.000 description 2
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000004727 humoral immunity Effects 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 210000004279 orbit Anatomy 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 2
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 2
- 229960001471 sodium selenite Drugs 0.000 description 2
- 239000011781 sodium selenite Substances 0.000 description 2
- 235000015921 sodium selenite Nutrition 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- 101710186708 Agglutinin Proteins 0.000 description 1
- 229930190011 Bufogenin Natural products 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- QEEBRPGZBVVINN-UHFFFAOYSA-N Desacetyl-bufotalin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C1(O)CCC2C=1C=CC(=O)OC=1 QEEBRPGZBVVINN-UHFFFAOYSA-N 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 238000001391 atomic fluorescence spectroscopy Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- QEEBRPGZBVVINN-BMPKRDENSA-N bufalin Chemical compound C=1([C@H]2CC[C@]3(O)[C@H]4[C@@H]([C@]5(CC[C@H](O)C[C@H]5CC4)C)CC[C@@]32C)C=CC(=O)OC=1 QEEBRPGZBVVINN-BMPKRDENSA-N 0.000 description 1
- ATLJNLYIJOCWJE-CWMZOUAVSA-N bufogenin Chemical compound C=1([C@H]2C[C@H]3O[C@@]43[C@H]3[C@@H]([C@]5(CC[C@H](O)C[C@H]5CC3)C)CC[C@@]42C)C=CC(=O)OC=1 ATLJNLYIJOCWJE-CWMZOUAVSA-N 0.000 description 1
- 229950006858 bufogenin Drugs 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 description 1
- 229960001008 heparin sodium Drugs 0.000 description 1
- 150000004678 hydrides Chemical class 0.000 description 1
- 230000005965 immune activity Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000004911 serous fluid Anatomy 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for artificially cultivating toads, which comprises the steps of cleaning toads by using extract liquid of mangnolia officinalis, immature bitter orange, mirabilite and rheum officinale, pulping the toads, adding a selenious acid triglyceride mixture and honey, placing a maggot cultivating box for chrysomyia megacephala to eat and grow maggots, promoting maggot production by using porous microspheres containing indole, giving a proper growth temperature to the maggots by adjusting the light, humidity and temperature in the maggot cultivating box, passing through water of 48-50 ℃, and naturally airing to obtain the toads; the cultured toad feeding insects have high utilization rate of effective active ingredients in toads, high selenium content and strong inhibition activity on malignant tumors; the yield is high, the quality is uniform, and the culture period is short; the method is simple and convenient, has no regional limitation, can ensure the stability of maggot raising conditions, and is suitable for long-term mass production.
Description
Technical Field
The invention relates to the field of toad feeding, in particular to a method for artificially culturing toad feeding.
Background
The toad maggot is a kind of maggot larva formed by laying fly on the dead body of toad and eating the maggot sprout out of toad meat, and is a traditional and precious animal in China. From ancient times to the present, a sentence of medicine proverb was introduced in the medical field: bufo frog can not produce maggot, and the produced maggot is treasure. Because the toad maggot has magical pharmacological action, it can cure several difficult and complicated diseases. As early as over 300 years ago, Li Shizhen has detailed and definite record on the unique medicinal value and magical curative effect of toad maggots in Ben Cao gang mu (Vol. fortieth).
The toad eating maggot is rich in a plurality of effective substances of the toads and the maggots, integrates the effects of the toads and the maggots, and actually takes fly maggots as carriers to selectively absorb and enrich effective components on the toads through the fly maggots. The Bufonis venenum mainly contains Bufonis venenum, chitin, natural agglutinin, various amino acids, protein, SOD, minerals, and bufalin, and has effects of tonifying heart, promoting urination, relieving pain, diminishing inflammation, and inhibiting tumor growth.
The function of the toad venom is very wide, but the toad venom has certain toxicity and is easy to be overdosed after being directly taken, so that the safety problem exists. The toad feeding is relatively safe, has the efficacy of toad venom and the function and efficacy of maggots, is a brand new compound product, and has been researched a lot.
Traditional toad eating insects are mainly naturally formed by wild accidentally dead toads, the toads eating insects collected in the past are bred by dead toads which are killed by livestock, bitten by wild animals and putrescent toads dead bodies after being killed by people, the toads eating insects are small in amount, can meet but cannot be obtained, are difficult to obtain stably and cannot meet a large number of market demands, patent CN1056956C discloses a method for artificially breeding the toad eating insects, the method is influenced by natural conditions, uncertain factors are more, the yield is limited, dirty matters in the toads are not removed, patent CN 104585 discloses a breeding method for the toad eating rejuvenation insects and prepared medicine raw materials, the breeding method is complicated, and the content of components in the toads eating insects is not uniform.
Disclosure of Invention
In view of the above situation, on the basis of the previous method, the artificial cultivation method of the toad feeding insects is provided, and the toad feeding insects with strong pharmacological activity and uniform component content can be quickly, conveniently, continuously and stably cultured in an industrialized mode.
The technical proposal for solving the problem is that,
an artificial cultivation method of toad feeding insects comprises the following steps:
s1, cleaning: cleaning living toads with clear water; then putting the toad into the mixed solution for 72 h;
s2, pulping: taking out the toad in S1, washing with flowing clear water for 20-24h, taking out the toad, beating the toad to cause dizziness, then pulping the toad uniformly, adding selenious acid triglyceride mixture and honey, and pulping the mixture uniformly again to obtain mixed toad pulp;
s3, maggot breeding: pouring the mixed toad slurry into a maggot raising pool in a maggot raising box, putting the porous microspheres into a grid box, putting chrysomyia megacephala into a maggot laying chamber, adjusting light, humidity and temperature, starting a ventilation device, starting a magnetic stirring device, continuously eating the mixed toad slurry by the chrysomyia megacephala and performing generation propagation, and obtaining the maximum toad eating number on the fifth day;
s4, maggot collection: turning off the light in the maggot laying chamber, turning on the light in the light chamber, allowing the toad to climb into the maggot collecting chamber through the pore channel, turning on a water sprayer in the maggot collecting chamber, after 2-3h, allowing the toad to climb in, and cleaning to obtain clean fresh maggots;
s5, putting the fresh maggots into water, heating to 48-50 ℃, filtering to remove water after 5-10min, and naturally airing for 2-3 days to obtain the toad eating maggots.
Further, the porous microspheres are porous activated carbon soaked in indole.
Further, the maggot raising box comprises a maggot producing chamber and a maggot collecting chamber, a maggot raising pool is arranged at the bottom of the maggot producing chamber, a plurality of grid boxes are arranged outside the maggot raising pool, pore passages are arranged outside the grid boxes, and the pore passages are communicated with the maggot producing chamber and the maggot collecting chamber.
Further, the preparation method of the porous microsphere comprises the following steps: firstly, indole is dissolved in ethanol to prepare the indole with the concentration of 1 × 10-4And (3) pouring the g/mL indole solution into porous activated carbon, immersing, stirring for 30-60min, and drying at 60-70 ℃ for 6-8h to obtain the porous microspheres.
Further, the maggot raising case comprises a maggot producing chamber and a maggot collecting chamber, the maggot producing chamber is arranged above the maggot collecting chamber, case shells are arranged outside the maggot producing chamber and the maggot collecting chamber, the case shells are black, the top of the maggot producing chamber is provided with an adjustable light, humidity detector, spraying device and air exchange device, a maggot raising pool is arranged at the bottom of the maggot producing chamber, magnetic balls are arranged in the maggot raising pool, a magnetic stirrer is arranged below the maggot raising pool, a plurality of net boxes are arranged outside the maggot raising pool, pore passages are arranged outside the net boxes and are communicated with the maggot producing chamber and the maggot collecting chamber, the lower edge of the pore passages is provided with an extension plate which extends outwards, the top of the maggot collecting chamber is provided with a water sprayer, light chambers are arranged at two sides of the maggot collecting chamber, a maggot blocking partition plate between the light chamber and the maggot collecting chamber is made of transparent glass, the maggot collecting box is arranged in the middle of the maggot collecting chamber, the cover-free position above the maggot collecting box, the transparent glass is made of transparent glass, a plurality of net holes are uniformly distributed on the maggot collecting box, the bottom of the maggot collecting chamber is provided with a water outlet.
Further, the preparation method of the mixed solution comprises the following steps: 100g of magnolia officinalis, 100g of immature bitter orange, 100g of mirabilite and 100g of rhubarb are taken, mixed and crushed, decocted in water for 30min and filtered, 25-30 kg of clean water is added into the filtrate, and the water temperature is kept at 26-28 ℃.
Further, the lamplight of the maggot producing chamber and the lamplight chamber is variable, before the toad pulp is put in, ultraviolet light is firstly used for irradiating for 5-10min, sterilization is carried out, after the toad pulp is put in, white light irradiation is carried out for 6:00-20:00, then the lamplight is turned off in 20:00-6:00 time period, the humidity is controlled to be 75% +/-5%, and the temperature is controlled to be 28 +/-2 ℃; the light of the light chamber is white light when maggots are collected.
Further, the mass quantity ratio of the mixed toad pulp to the chrysomyia megacephala is 1000g/200, wherein the quantity ratio of male and female of the chrysomyia megacephala is 1: 1; the female chrysomyia megacephala is to-be-born chrysomyia megacephala.
Furthermore, after maggots are collected, chrysomyia megacephala imagoes in the maggot breeding box can be continuously used for breeding toads in the next round.
Furthermore, the selenious acid triglyceride mixture is synthesized by esterification of sunflower oil and selenious acid, the content of the selenious acid triglyceride is 2%, and the selenious acid triglyceride mixture mainly has a dioxaselenolane structure.
Further, the mass volume ratio of the toad, selenious acid triglyceride mixture and honey is as follows: 800-1000g, 20-24g, 50-60 g.
Further, the toad variety is a Chinese toad.
Due to the adoption of the technical scheme, compared with the prior art, the invention has the following advantages;
the toad larvae cultured by the method for artificially culturing the toad larvae has high utilization rate of effective active ingredients in the toads, high selenium content and stronger inhibitory activity on malignant tumors; the yield is high, the quality is uniform, and the culture period is short; the method is simple and convenient, has no regional limitation, can ensure the stability of maggot raising conditions, and is suitable for long-term mass production.
Drawings
FIG. 1 is a structural view of a maggot raising box according to the present invention;
FIG. 2 is a sectional view of the maggot raising box of the present invention.
Detailed Description
The foregoing and other technical and scientific aspects, features and utilities of the present invention will be apparent from the following detailed description of the embodiments, which is to be read in connection with the accompanying drawings of fig. 1-2. The structural contents mentioned in the following embodiments are all referred to the attached drawings of the specification.
Example 1
An artificial cultivation method of toad feeding insects comprises the following steps:
s1, cleaning: washing 16kg of living toad with clear water; then putting the toad into the mixed solution for 72 h;
s2, pulping: taking out the toads in the S1, washing the toads with flowing clear water for 20h, taking out the toads, beating the toads to cause dizziness, then pulping the toads uniformly, adding 40g of selenious acid triglyceride mixture and 100g of honey, and pulping the toads uniformly again to obtain mixed toad pulp;
s3, maggot breeding: pouring the mixed toad slurry into a maggot raising pool in a maggot raising box, putting the porous microspheres into a grid box 15, putting chrysomyia megacephala into a maggot laying chamber, adjusting light, humidity and temperature, starting a ventilation device, starting a magnetic stirring device, continuously eating the mixed toad slurry by the chrysomyia megacephala and performing generation and propagation, and obtaining the maximum number of toads eating on the fifth day;
s4, maggot collection: turning off the light in the maggot laying chamber, turning on the light in the light chamber, allowing the toad to climb into the maggot collecting chamber through the pore channel, turning on a water sprayer in the maggot collecting chamber, after 2h, allowing the toad to climb in, and cleaning to obtain clean fresh and alive maggots;
s5, putting the fresh maggots into water, heating to 48 ℃, filtering to remove water after 5min, and naturally airing for 2 days to obtain the toad food.
After maggots are collected, chrysomyia megacephala imagoes in the maggot breeding box can be continuously used for breeding toads in the next round.
The preparation method of the porous microsphere comprises the following steps: firstly, indole is dissolved in ethanol to prepare the indole with the concentration of 1 × 10-4And (3) pouring the indole solution of g/mL into porous activated carbon, immersing, stirring for 30min, and drying for 6h at 60 ℃ to obtain the porous microspheres.
As shown in figure 1 and figure 2, the maggot raising case comprises a maggot producing chamber and a maggot collecting chamber, wherein the maggot producing chamber is arranged above the maggot collecting chamber, a case shell 1 is arranged outside the maggot producing chamber and the maggot collecting chamber, the case shell 1 is black, the top of the maggot producing chamber is provided with adjustable light 10, a humidity detector 20, a spraying device 12 and a ventilation device 11, a maggot raising pool 13 is arranged at the bottom of the maggot producing chamber, magnetic balls 19 are arranged in the maggot raising pool 13, a magnetic stirrer 18 is arranged below the maggot raising pool, a plurality of net boxes 15 are arranged outside the maggot raising pool, a pore passage 16 is arranged outside the net boxes 15, the pore passage 16 is communicated with the maggot producing chamber and the maggot collecting chamber, an extension plate 17 is arranged at the lower edge of the pore passage 16, the extension plate 17 extends outwards, a water sprayer 30 is arranged at the top of the maggot collecting chamber, light chambers 31 are arranged at two sides of the maggot collecting chamber, a baffle plate between the light chamber 31 and the maggot collecting chamber is made of transparent glass, a collection box 32 is arranged in the middle of the maggot collecting chamber, the collection box is made of transparent glass, a plurality of net holes are uniformly distributed on the maggot collecting box 32, the net holes do not allow the smallest maggots to pass through, and a water outlet 33 is arranged at the bottom of the maggot collecting chamber.
The preparation method of the mixed solution comprises the following steps: 100g of magnolia officinalis, 100g of immature bitter orange, 100g of mirabilite and 100g of rhubarb are taken, mixed and crushed, decocted in water for 30min and filtered, 25 kg of clear water is added into the filtrate, and the water temperature is kept at 26 ℃.
Before the toad slurry is put in, the lamp of a maggot breeding box is firstly adjusted to be ultraviolet light for irradiating and sterilizing for 5min, after the toad slurry is put in, white light is irradiated for 6:00-20:00, then the lamp light is turned off in 20:00-6:00 time period, the humidity is controlled at 70%, the temperature is controlled at 26 ℃, and the rotating speed of magnetic stirring is 200 r/min.
The mass quantity ratio of the mixed toad slurry to the chrysomyia megacephala is 1000g/200, wherein the quantity ratio of male to female of the chrysomyia megacephala is 1: 1; the female chrysomyia megacephala is to-be-born chrysomyia megacephala.
The selenious acid triglyceride mixture is synthesized by esterification of sunflower oil and selenious acid, the content of the selenious acid triglyceride is 2%, and the selenious acid triglyceride mixture mainly has a dioxaselenolane structure.
The toad variety is Bufo siccus.
Example 2
An artificial cultivation method of toad feeding insects comprises the following steps:
s1, cleaning: washing 18kg of living toad with clear water; then putting the toad into the mixed solution for 72 h;
s2, pulping: taking out the toads in the S1, washing the toads with flowing clear water for 22h, taking out the toads, beating the toads to cause dizziness, then pulping the toads uniformly, adding 44g of selenious acid triglyceride mixture and 110g of honey, and pulping the toads uniformly again to obtain mixed toad pulp;
s3, maggot breeding: pouring the mixed toad slurry into a maggot raising pool in a maggot raising box, putting the porous microspheres into a grid box, putting chrysomyia megacephala into a maggot laying chamber, adjusting light, humidity and temperature, starting a ventilation device, starting a magnetic stirring device, continuously eating the mixed toad slurry by the chrysomyia megacephala and performing generation propagation, and obtaining the maximum toad eating number on the fifth day;
s4, maggot collection: turning off the light in the maggot laying chamber, turning on the light in the light chamber, allowing the toad to climb into the maggot collecting chamber through the pore channel, turning on a water sprayer in the maggot collecting chamber, after 2.5h, allowing the toad to climb in, and cleaning to obtain clean fresh maggots;
s5, putting the fresh maggots into water, heating to 49 ℃, filtering to remove water after 8min, and naturally airing for 3 days to obtain the toad food.
After maggots are collected, chrysomyia megacephala imagoes in the maggot breeding box can be continuously used for breeding toads in the next round.
The preparation method of the porous microsphere comprises the following steps: firstly, indole is dissolved in ethanol to prepare the indole with the concentration of 1 × 10-4And (3) pouring the indole solution of g/mL into porous activated carbon, immersing, stirring for 45min, and drying for 7h at 65 ℃ to obtain the porous microspheres.
The preparation method of the mixed solution comprises the following steps: 100g of magnolia officinalis, 100g of immature bitter orange, 100g of mirabilite and 100g of rhubarb are taken, mixed and crushed, decocted in water for 30min and filtered, 28 kg of clear water is added into the filtrate, and the water temperature is kept at 27 ℃.
Before the toad slurry is put in, the lamp of a maggot breeding box is firstly adjusted to be ultraviolet light for irradiating and sterilizing for 8min, after the toad slurry is put in, white light is irradiated for 6:00-20:00, then the lamp light is turned off in 20:00-6:00 time period, the humidity is controlled at 75%, the temperature is controlled at 28, and the rotating speed of magnetic stirring is 250 r/min.
The mass quantity ratio of the mixed toad slurry to the chrysomyia megacephala is 1000g/200, wherein the quantity ratio of male to female of the chrysomyia megacephala is 1: 1; the female chrysomyia megacephala is to-be-born chrysomyia megacephala.
The selenious acid triglyceride mixture is synthesized by esterification of sunflower oil and selenious acid, the content of the selenious acid triglyceride is 2%, and the selenious acid triglyceride mixture mainly has a dioxaselenolane structure.
The toad variety is Bufo siccus.
Example 3
An artificial cultivation method of toad feeding insects comprises the following steps:
s1, cleaning: washing 20kg of living toad with clear water; then putting the toad into the mixed solution for 72 h;
s2, pulping: taking out the toads in the S1, washing the toads with flowing clear water for 24h, taking out the toads, beating the toads to cause dizziness, then pulping the toads uniformly, adding 48g of selenious acid triglyceride mixture and 120g of honey, and pulping the toads uniformly again to obtain mixed toad pulp;
s3, maggot breeding: pouring the mixed toad slurry into a maggot raising pool in a maggot raising box, putting the porous microspheres into a grid box, putting chrysomyia megacephala into a maggot laying chamber, adjusting light, humidity and temperature, starting a ventilation device, starting a magnetic stirring device, continuously eating the mixed toad slurry by the chrysomyia megacephala and performing generation propagation, and obtaining the maximum toad eating number on the fifth day;
s4, maggot collection: turning off the light in the maggot laying chamber, turning on the light in the light chamber, allowing the toad to climb into the maggot collecting chamber through the pore channel, turning on a water sprayer in the maggot collecting chamber, after 3h, allowing the toad to climb in, and cleaning to obtain clean fresh and alive maggots;
s5, putting the fresh maggots into water, heating to 50 ℃, filtering to remove water after 10min, and naturally airing for 3 days to obtain the toad food.
After maggots are collected, chrysomyia megacephala imagoes in the maggot breeding box can be continuously used for breeding toads in the next round.
The preparation method of the porous microsphere comprises the following steps: firstly, indole is dissolved in ethanol to prepare the indole with the concentration of 1 × 10-4And (3) pouring the indole solution of g/mL into porous activated carbon, immersing, stirring for 60min, and drying for 8h at 70 ℃ to obtain the porous microspheres.
The preparation method of the mixed solution comprises the following steps: 100g of magnolia officinalis, 100g of immature bitter orange, 100g of mirabilite and 100g of rhubarb are taken, mixed and crushed, decocted in water for 30min and filtered, 30 kg of clear water is added into the filtrate, and the water temperature is kept at 28 ℃.
Before the toad slurry is put in, the lamp of a maggot breeding box is adjusted to be ultraviolet light to irradiate and sterilize for 5-10min, after the toad slurry is put in, white light irradiation is carried out for 6:00-20:00, then the lamp light is turned off in 20:00-6:00 time period, the humidity is controlled at 80%, the temperature is controlled at 30 ℃, and the rotating speed of magnetic stirring is 300 r/min.
The mass quantity ratio of the mixed toad slurry to the chrysomyia megacephala is 1000g/200, wherein the quantity ratio of male to female of the chrysomyia megacephala is 1: 1; the female chrysomyia megacephala is to-be-born chrysomyia megacephala.
The selenious acid triglyceride mixture is synthesized by esterification of sunflower oil and selenious acid, the content of the selenious acid triglyceride is 2%, and the selenious acid triglyceride mixture mainly has a dioxaselenolane structure.
The toad variety is Bufo siccus.
Comparative example 1
The selenious acid triglyceride mixture in the step of artificially culturing toad feeding in example 1 is replaced by sodium selenite, and the rest steps are unchanged.
Comparative example 2
The honey in the step of artificially culturing toad feeding in example 1 is removed, and the rest steps are unchanged.
Comparative example 3
The porous microspheres in the step of artificially culturing toad feeding in example 1 are removed, and the rest steps are unchanged.
Comparative example 4
The step of artificially culturing toad feeding in example 1, "S2, pulping: taking out the toad in the S1, cleaning the toad with flowing clear water for 20h, taking out the toad, beating the toad to be stunned, then beating the toad uniformly, adding selenious acid triglyceride mixture and honey, beating the mixture uniformly again to obtain mixed toad pulp, replacing the mixed toad pulp with S2, and pulping: taking out Bufo siccus in S1, washing with flowing clear water for 20 hr, taking out Bufo siccus, beating to make it dizzy, adding selenious acid triglyceride mixture and Mel into Bufo siccus body to obtain mixed Bufo siccus slurry, and keeping the rest steps unchanged.
Comparative example 5
The selenious acid triglyceride mixture in the step of artificially culturing toad feeding in example 1 is removed, and the rest steps are unchanged.
Test example 1
The quality of the toad feeding insects obtained in examples 1-3 and comparative examples 1-5 was recorded, respectively, and the toad feeding yield (toad feeding quality/toad quality) was calculated;
the average selenium content in the toad larvae of examples 1 to 3 and comparative examples 1 to 5 was determined by hydride atomic fluorescence spectrometry according to determination of selenium in national standard food for food safety (GB 5009.93-2017);
randomly selecting 100 toad pests in each of examples 1-3 and comparative examples 1-5, determining the content of the toad peptide in each toad pest according to the method for determining the toad peptide in CN102091318A, and recording the lowest content and the highest content;
100 toad feeding insects in each of examples 1 to 3 and comparative examples 1 to 5 were randomly selected, and the content of the toad active ingredients in the toad feeding insects was expressed by toad venom base content measurement, wherein the measurement method is as follows: quality analysis of toad fed and maggots [ J ] Shenyang pharmaceutical university report, 2013(5), recording minimum and maximum content;
100 toads are randomly selected in each of examples 1 to 3 and comparative examples 1 to 5, the total nitrogen content in the toads is used as the protein content, the total nitrogen content is measured for each toad according to the requirement of the second method of the first appendix IX L of the 2010 edition of the pharmacopoeia of the people's republic of China, and the lowest content and the highest content are recorded.
The test results are shown in Table 1
From table 1, the toad feeding insects obtained in examples 1 to 3 have high yield, high content of bufopeptide and bufogenin, uniform quality, high effective absorption to toads, selenious acid triglyceride is more suitable for enrichment of selenium element by toads, and sodium selenite can also enrich selenium of toads, but has high mortality rate and low final yield; the honey can promote chrysomyia megacephala to soak the mixed toad serous fluid and can help to improve the selenium content and the active ingredients of the toad eating insects; the porous microspheres contain indole, so that the chrysomyia megacephala can be promoted to lay eggs, the egg laying period is shortened, and the yield is improved; compared with the direct eating of toad carcasses, the mixed toad slurry can better ensure the uniform quality of the toad eating insects from the maximum difference content of the toad peptide and the toad venom base.
Test example 2
The toad feeding immune activity research:
experiment one: 120 mice, half male and female, weighing 18-22g, were randomly divided into 4 groups: group I, group II, group III and control group, wherein the toad feeding in example 1 is group I, the toad feeding in comparative example 5 is group II, and the toad feeding in comparative example 5 is group III by adding 2.8% of selenoprotein, pulverizing into fine powder, sieving with 80 mesh sieve, and mixing with 1% of sodium carboxymethyl cellulose to obtain suspension; 0.5mL of 1% sodium carboxymethyl cellulose solution is irrigated to a control group, 0.5mL of 0.332% suspension is irrigated to a group I, a group II and a group III respectively every day, the mice are continuously fed for 15 days, blood is bled from eye sockets of the mice after the last feeding, the animals are sacrificed, the weights of the body weight, the thymus, the liver and the spleen are weighed respectively, and the organ coefficient is calculated;
experiment two: four groups of mice fed in experiment one were bled from the orbit and placed in anticoagulated test tubes with 1% heparin sodium to isolate plasma. Taking 0.1mL of blood plasma to be detected, adding 0.3mL of physiological saline, uniformly mixing, taking 10uL, injecting into a G plate hole, placing in a horizontal wet box, placing in an incubator at 37 ℃, observing the result after 48 hours, reading the diameter of a diffusion ring of each hole by using an immunity measuring instrument, and calculating the content of IgG by using a standard curve;
experiment three: and adding 0.1mL of anticoagulation blood of each group in experiment II into 1.8mL of RPMll640 culture medium, simultaneously adding 0.1mL of PHA into a 37 ℃ water bath for 72h, sucking the supernatant, adding 4mL of human N trampling CL (8.39L /) and uniformly mixing the mixture, carrying out the water bath for 10min, centrifuging the mixture for 10min, discarding the supernatant, adding 5mL of stationary liquid, standing the mixture for 10min at room temperature, and centrifuging the mixture to discard the supernatant. Soaking the glass slide in distilled water, placing the glass slide in a refrigerator, adding 2 drops of precipitate into the glass slide, naturally drying and dyeing, calculating the number of 100 lymphocytes, and calculating the conversion rate;
the results are shown in Table 2:
from table 2, the weight of important immune organs such as thymus and spleen of mice in groups I-III is obviously reduced, immunoglobulin production and lymphocyte transformation rate are also inhibited to a certain extent, which indicates that the toad feeding insects have the same inhibitory action on body humoral immunity and cellular immunity, the mechanism is consistent with that of clinical application of toad feeding insects for treating malignant tumors, compared with the toad feeding insects in comparative example 5 and the mixture of toad feeding insects and selenoprotein in comparative example 5, the toad feeding insects prepared by the method have stronger inhibitory action on body humoral immunity and cellular immunity of mice, and the toad feeding insects prepared by the method have stronger inhibitory activity on malignant tumors.
While the invention has been described in further detail with reference to specific embodiments thereof, it is not intended that the invention be limited to the specific embodiments thereof; for those skilled in the art to which the present invention pertains and related technologies, the extension, operation method and data replacement should fall within the protection scope of the present invention based on the technical solution of the present invention.
Claims (9)
1. The method for artificially culturing toad feeding insects is characterized by comprising the following steps:
s1, cleaning: cleaning living toads with clear water; then putting the toad into the mixed solution for 72 h;
s2, pulping: taking out the toad in S1, washing with flowing clear water for 20-24h, taking out the toad, beating the toad to cause dizziness, then pulping the toad uniformly, adding selenious acid triglyceride mixture and honey, and pulping the mixture uniformly again to obtain mixed toad pulp;
s3, maggot breeding: pouring the mixed toad slurry into a maggot raising pool in a maggot raising box, putting the porous microspheres into a grid box, putting chrysomyia megacephala into a maggot laying chamber, adjusting light, humidity and temperature, starting a ventilation device, starting a magnetic stirring device, continuously eating the mixed toad slurry by the chrysomyia megacephala and performing generation propagation, and obtaining the maximum toad eating number on the fifth day;
s4, maggot collection: turning off the light in the maggot laying chamber, turning on the light in the light chamber, allowing the toad to climb into the maggot collecting chamber through the pore channel, turning on a water sprayer in the maggot collecting chamber, after 2-3h, allowing the toad to climb in, and cleaning to obtain clean fresh maggots;
s5, putting the fresh maggots into water, heating to 48-50 ℃, filtering to remove water after 5-10min, and naturally airing for 2-3 days to obtain toad eating maggots;
the porous microspheres are porous activated carbon soaked in indole; the maggot raising case comprises a maggot raising chamber and a maggot collecting chamber, wherein a maggot raising pool is arranged at the bottom of the maggot raising chamber, a plurality of grid boxes are arranged outside the maggot raising pool, pore passages are arranged outside the grid boxes, and the pore passages are communicated with the maggot raising chamber and the maggot collecting chamber.
2. The method for artificially culturing toad feeding according to claim 1, wherein the porous microspheres are prepared by the following steps: firstly, indole is dissolved in ethanol to prepare the indole with the concentration of 1 × 10-4And (3) pouring the g/mL indole solution into porous activated carbon, immersing, stirring for 30-60min, and drying at 60-70 ℃ for 6-8h to obtain the porous microspheres.
3. The method for artificially culturing toads according to claim 1, wherein the maggot raising case comprises a maggot producing chamber and a maggot collecting chamber, the maggot producing chamber is arranged above the maggot collecting chamber, case shells are arranged outside the maggot producing chamber and the maggot collecting chamber, the case shells are black, an adjustable light, a humidity detector, a spraying device and a ventilation device are arranged at the top of the maggot producing chamber, a maggot raising pool is arranged at the bottom of the maggot producing chamber, magnetic balls are arranged in the maggot raising pool, a magnetic stirrer is arranged below the maggot raising pool, a plurality of grid boxes are arranged outside the maggot raising pool, a pore passage is arranged outside each grid box, the pore passage is communicated with the maggot producing chamber and the maggot collecting chamber, an extension plate is arranged at the lower edge of the pore passage, the extension plate extends outwards, a water sprayer is arranged at the top of the maggot collecting chamber, light chambers are arranged at two sides of the maggot collecting chamber, a baffle plate between the light chamber and the maggot collecting chamber is made of transparent glass, the maggot collecting box is arranged in the middle of the maggot collecting chamber, a cover is not arranged above the collecting box made of transparent glass, a plurality of net holes are uniformly distributed on the maggot collecting box, the net holes do not allow the smallest maggots to pass through, and a water outlet is arranged at the bottom of the maggot collecting chamber.
4. The method for artificially culturing toad feeding according to claim 1, wherein the preparation method of the mixed solution comprises: 100g of magnolia officinalis, 100g of immature bitter orange, 100g of mirabilite and 100g of rhubarb are taken, mixed and crushed, decocted in water for 30min and filtered, 25-30 kg of clean water is added into the filtrate, and the water temperature is kept at 26-28 ℃.
5. The method for artificially cultivating and feeding toad larvae, as claimed in claim 1, wherein the light of both the maggot-producing chamber and the light chamber is variable, ultraviolet light is used for 5-10min before toad slurry is put in, sterilization is carried out, white light is used for 6:00-20:00 after toad slurry is put in, then the light is turned off in 20:00-6:00, the humidity is controlled at 75% ± 5%, and the temperature is controlled at 28 ± 2 ℃; the light of the light chamber is white light when maggots are collected.
6. The method for artificial cultivation of toad feeding insects as claimed in claim 1, wherein the mass quantity ratio of the mixed toad slurry to the Chrysomya megacephala is 1000g/200, wherein the ratio of the Chrysomya megacephala to the Chrysomya megacephala is 1: 1; the female chrysomyia megacephala is to-be-born chrysomyia megacephala.
7. The method for artificially culturing and feeding toads according to claim 1, wherein the chrysomyia megacephala imagoes in the maggot raising box can be continuously used for culturing the toads in the next round after collecting the maggots.
8. The method for artificially culturing and feeding toad pests according to claim 1, wherein the selenious acid triglyceride mixture is synthesized by esterification of sunflower oil and selenious acid, the selenious acid triglyceride content is 2%, and the selenious acid triglyceride mixture mainly has a dioxaselenolane structure.
9. The method for artificially culturing and feeding the toads according to claim 1, wherein the mass-volume ratio of the toads to the selenious acid triglyceride mixture to the honey is as follows: 800-1000g, 20-24g, 50-60 g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110992506.5A CN113455470B (en) | 2021-08-27 | 2021-08-27 | Method for artificially culturing toad feeding insects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110992506.5A CN113455470B (en) | 2021-08-27 | 2021-08-27 | Method for artificially culturing toad feeding insects |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113455470A CN113455470A (en) | 2021-10-01 |
| CN113455470B true CN113455470B (en) | 2022-03-08 |
Family
ID=77866939
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110992506.5A Active CN113455470B (en) | 2021-08-27 | 2021-08-27 | Method for artificially culturing toad feeding insects |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113455470B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT4869U1 (en) * | 1998-10-20 | 2001-12-27 | Kling Frank | AMPHIBIAN FENCE AS A GUIDE FOR TOADS, FROGS AND THE LIKE AMPHIBIAS |
| CN1736186A (en) * | 2005-08-04 | 2006-02-22 | 周志钦 | Method for breeding fly maggot with anti-tumor biological characteristic |
| CN101491231A (en) * | 2009-02-26 | 2009-07-29 | 袁正洋 | Collection method of selenium-rich toad-ecdysis |
| CN102078593A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotic for preparing medicines for curing diabetic foot |
| CN102078592A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotics in preparing drugs for treating acnes |
| CN102273430A (en) * | 2010-06-09 | 2011-12-14 | 张春燕 | Culturing method of maggot containing toad toxication component and treating function of preparation of maggot |
| CN102578034A (en) * | 2012-03-07 | 2012-07-18 | 安徽省旌德博仕达农业科技有限公司 | Toad breeding device and toad venom acquisition method |
| CN104285913A (en) * | 2014-11-04 | 2015-01-21 | 凌征雄 | Housefly breeding method |
| CN104663585A (en) * | 2013-12-02 | 2015-06-03 | 黑龙江省传奇生物医药科技开发有限公司 | Cultivation method of toad-fed maggot and prepared drug raw material of toad-fed maggot |
| CN112369372A (en) * | 2020-11-16 | 2021-02-19 | 叶守成 | Method for breeding maggots fed by toads |
-
2021
- 2021-08-27 CN CN202110992506.5A patent/CN113455470B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT4869U1 (en) * | 1998-10-20 | 2001-12-27 | Kling Frank | AMPHIBIAN FENCE AS A GUIDE FOR TOADS, FROGS AND THE LIKE AMPHIBIAS |
| CN1736186A (en) * | 2005-08-04 | 2006-02-22 | 周志钦 | Method for breeding fly maggot with anti-tumor biological characteristic |
| CN101491231A (en) * | 2009-02-26 | 2009-07-29 | 袁正洋 | Collection method of selenium-rich toad-ecdysis |
| CN102273430A (en) * | 2010-06-09 | 2011-12-14 | 张春燕 | Culturing method of maggot containing toad toxication component and treating function of preparation of maggot |
| CN102078593A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotic for preparing medicines for curing diabetic foot |
| CN102078592A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotics in preparing drugs for treating acnes |
| CN102578034A (en) * | 2012-03-07 | 2012-07-18 | 安徽省旌德博仕达农业科技有限公司 | Toad breeding device and toad venom acquisition method |
| CN104663585A (en) * | 2013-12-02 | 2015-06-03 | 黑龙江省传奇生物医药科技开发有限公司 | Cultivation method of toad-fed maggot and prepared drug raw material of toad-fed maggot |
| CN104285913A (en) * | 2014-11-04 | 2015-01-21 | 凌征雄 | Housefly breeding method |
| CN112369372A (en) * | 2020-11-16 | 2021-02-19 | 叶守成 | Method for breeding maggots fed by toads |
Non-Patent Citations (2)
| Title |
|---|
| 不同硒源和剂量对成年马的代谢和;郭永清;《中国饲料》;20201231;第68-71页 * |
| 食蟾虫临床运用初探;王宗殿;《河北中医药学报》;20001231;第32-33页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113455470A (en) | 2021-10-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101606508A (en) | The method of ecologically breeding black-bone chickens in medlar garden | |
| CN103130909A (en) | Preparation method of selenium-rich Morchella polysaccharide | |
| CN107593603A (en) | Toad is classified ecological cultivation method | |
| CN106818438A (en) | A kind of method of the floating bed cage culture swamp eel using vegetables of sheltering from heat or light | |
| CN111616101A (en) | Ecological breeding method of osmanthus fragrans chickens | |
| CN110074226A (en) | Improve the production method of wild worm tea purity | |
| CN103168750A (en) | Method of reducing amount of plutella xylostella through propagation and field release of cotesia plutellae | |
| CN102144610B (en) | Snake hibernation-free high-efficiency breeding method | |
| CN109769755A (en) | A kind of simple and fast propagation method of spotted wing drosophila pupa parasitic wasp hair angle clavicorn serphid | |
| CN102172152A (en) | Method for improving dendrobium candidum polysaccharide | |
| CN109220907A (en) | A kind of excellent production breeding method of madai | |
| CN113455470B (en) | Method for artificially culturing toad feeding insects | |
| CN101491231A (en) | Collection method of selenium-rich toad-ecdysis | |
| CN105995249A (en) | Banana prawn feed additive and preparation method thereof | |
| CN109504609B (en) | Method for culturing seaweed for feeding plankton | |
| Tasaki | Resting and action potentials of reversed polarity in frog nerve cells | |
| CN101735329A (en) | Pholiota adiosapose polysaccharide and preparation method thereof | |
| CN108812165A (en) | A kind of high-yield planting method of dendrobium nobile | |
| CN101049161B (en) | Silkworm breeding method in space base as source of animal protein in human dietary | |
| CN114885870A (en) | Spawning induction method for sipunculus australis | |
| CN109743926B (en) | Green and total flavone-enriched broccoli sprout cultivation method | |
| CN106173551A (en) | A kind of holothurian feed additive and preparation method thereof | |
| CN109258563A (en) | A kind of toad Ecology breeding method | |
| CN110521669B (en) | Green giant spiny frog breeding method | |
| CN102090354A (en) | Method for artificially culturing corbicula fluminea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method of artificially cultivating toad eaters Effective date of registration: 20220711 Granted publication date: 20220308 Pledgee: Mudanjiang Zhongrong Financing Guarantee Co.,Ltd. Pledgor: Shanghai hanyue Pharmaceutical Biotechnology Development Co.,Ltd. Registration number: Y2022230000036 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230727 Granted publication date: 20220308 Pledgee: Mudanjiang Zhongrong Financing Guarantee Co.,Ltd. Pledgor: Shanghai hanyue Pharmaceutical Biotechnology Development Co.,Ltd. Registration number: Y2022230000036 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Method for Artificial Cultivation of Toad Eating Insects Effective date of registration: 20230811 Granted publication date: 20220308 Pledgee: Mudanjiang Zhongrong Financing Guarantee Co.,Ltd. Pledgor: Shanghai hanyue Pharmaceutical Biotechnology Development Co.,Ltd. Registration number: Y2023310000454 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230927 Address after: Room 106, Building F3, Yanhe South, Dongshan District, Hegang City, Heilongjiang Province, 154100 Patentee after: Heilongjiang Chanbao Toad Breeding Co.,Ltd. Address before: 201500 building 3, No. 228, jingle Road, Langxia Town, Jinshan District, Shanghai (Langxia Lenong cultural and Creative Industrial Park) Patentee before: Shanghai hanyue Pharmaceutical Biotechnology Development Co.,Ltd. |
|
| TR01 | Transfer of patent right |