CN101491231A - Collection method of selenium-rich toad-ecdysis - Google Patents
Collection method of selenium-rich toad-ecdysis Download PDFInfo
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- CN101491231A CN101491231A CNA2009100246941A CN200910024694A CN101491231A CN 101491231 A CN101491231 A CN 101491231A CN A2009100246941 A CNA2009100246941 A CN A2009100246941A CN 200910024694 A CN200910024694 A CN 200910024694A CN 101491231 A CN101491231 A CN 101491231A
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Abstract
The invention provides a method for collecting selenium enriched toad skins. A toad culture stage comprises the following steps: adding the fodder with the high selenium content 5 to 7 days after the tadpole period, and culturing phycophyta with the high selenium content in the tadpole pond; feeding the bait with the high selenium content and matched fodder in the child toad period; and applying selenium enriched fertilizer to the terrestrial soil of the adult toad pond according to the dosage of 0.4 to 0.6 kg/hm<2>, planting farm crops or vegetables, and feeding artificially cultured bait. A toad skin collecting stage comprises the following steps: adding selenium enriched yeast into the water of an ecdysis pond so as to prevent the selenium contained by the toad from losing; drying the toad skin to the water content less than or equal to 5 percent; and packaging the toad skin into bags after the disinfection through ultraviolet rays so as to obtain the selenium enriched toad skin. The method adds the selenium enriched bait and provides a selenium enriched growing environment in the toad culture process from the child toad to the adult toad, thereby making the toad enriching selenium, and ensuring no loss of selenium in the ecdysis process. Therefore, the toad skin has the high selenium content. The method remarkably improves the medical and functional value of the toad skin.
Description
Technical field
The present invention relates to a kind of collecting method of selenium-rich toad-ecdysis.
Background technology
Toad is the very high wildlife of a kind of medical value, and is just on the books in the medical masterpieces such as Compendium of Material Medica that Ming Dynasty's Li Shizhen (1518-1593 A.D.) is write.Traditional method is to be used as medicine with the dried toad (the dry body of the toad that gills) and the dried venom of toads (dry product of extruding toad ear rear gland and glandula integumentaria gained milky white liquid).But obtain dried toad and just must kill toad, this method of obtaining the medicine source also is unfavorable for saving the wild animals and maintaining ecological balance undoubtedly in sacrificing future gains to satisfy present needs.And the collection of the dried venom of toads is comparatively difficult, and output is also very low.
The toad clothing is the cutin clothing film that toad is taken off naturally.Sun Simiao claims: " toad is sloughed off (clothing), removes to dislike and swells, and god also ".Li Shizhen (1518-1593 A.D.) " this order guiding principle wood " claims: " the toad clothing is its vital essence of holding sufficient the five internal organs the bottom of heart, receives the China of world negative and positive precious, if one of obtain, all foul diseases do not have and do not heal ".The traditional Chinese medical science is thought: the toad clothing has detoxifcation, detumescence, pain relieving, wards off foul effect, is widely used in the treatment of sore pain pyogenic infections, abscess of throat etc.Modern study is thought: toad clothing master contains toad dienol compound, comprises bufalin and arenobufagin, has cardiac stimulant, boosts, antiinflammatory action, and bufalin also has stronger anesthetic effect.Current research shows that also the toad clothing also has magical effects such as antitumor, antiviral, can treat difficult and complicated cases such as multiple malignant tumour, hepatitis, herpes zoster, liver ascites, ephrosis, fibroid.
Selenium is the human body trace elements necessary, and very important physical function is arranged, and is closely related with human beings'health.Selenium can prevent and suppress tumour, anti-ageing, the normal function of keeping cardiovascular system, the appearance of prevention of arterial sclerosis and coronary heart disease.Scarce selenium can cause Keshan disease and big bone knot disease, also can bring out cataract, hepatopathy and pancreas disease etc.
Summary of the invention
The collecting method that the purpose of this invention is to provide a kind of selenium-rich toad-ecdysis, it is by the rich selenium food of absorption in the toad process of growth, and adds rich selenium carrier during toad sloughs off clothing, guarantees that the selenium that toad is replenished does not run off, and the acquisition selenium-rich toad-ecdysis, to improve the medicinal and functional effect of toad clothing.
The technical solution adopted for the present invention to solve the technical problems is: it comprises toad breed stage and toad clothing acquisition phase;
Toad is cultured the stage:
(1), the tadpole phase: at the water purification of tadpole pond injection behind the Exposure to Sunlight aeration, water temperature is 18～24 ℃, the depth of water 20～40cm; Cultivate the high algae of selenium content in the tadpole pond; Implant the pasture and water that account for the water surface 40～60% simultaneously, perch and play the effect of sunshade for tadpole; Add high yellow mealworm powder or the goose powdered egg of selenium content after 5～7 days, or living goose egg, feeding volume is 7%～10% of a tadpole body weight;
(2), the toadlet phase: the toadlet pond is that Tu Chi or pond transformation form, and after carrying out disinfection and treating that toxicity disappears, injects the water of 20～40cm behind the Exposure to Sunlight aeration; The periphery in toadlet pond is provided with anti-divider wall or the protection network of escaping, preventing the string pond; Cultivate the high algae of selenium content in the toadlet pond, toadlet except that the natural living bait of ingesting, the high living bait of selenium content of also throwing something and feeding, the living bait amount of throwing something and feeding day is 10～15% of a toadlet body weight; And, the manufactured feed of also throwing something and feeding, the manufactured feed amount of throwing something and feeding day is 7～10% of a toadlet body weight;
(3), become the toad phase: become toad based on land-based activities, terrestrial soil is by 0.4～0.6kg/hm
2Use rich selenium fertilizer, and plantation crops or vegetables, produce selenium-enriched crop and grow rich selenium bait; Build awning simultaneously, light trap is installed, the brick bits clitter that multiple hole is set is perched for toad, and equipment of sprinkler irrigation also is installed, for toad provide one quietly, plants burst out in profusion and moist terrestrial environment; The assorted worm of natural world that rich selenium bait that crops grow in the one-tenth toad predation plant and light trap are lured, and the yellow mealworm of the artificial culture of throwing something and feeding and fly maggot, injected volume is into 10～15% of toad body weight;
Toad clothing acquisition phase:
(1), catches: catch many, the individual adult toads more than 100g of back stain;
(2), clean: toad is placed service sink, extremely clean with pollution-free neutral fresh water buffering toad;
(3), excite mucous membrane: toad is placed in the container of dry cleansing, not feeding, do not spray water, it is moistening by drying toad secretion mucous membrane to epidermis to be kept, 40～50 hours, the toad clothing selenium content content height under sloughing off like this;
(4), slough off clothing: toad is placed slough off the clothing pond, inject pollution-free neutral fresh water in the pond, 25～35 centimetres of the depth of waters add Se-enriched yeast in the water, and the weight ratio of water and Se-enriched yeast is 25～35: 1; Toad sloughs off clothing after 40～50 hours in sloughing off the clothing pond and since during drink for rich selenium water, its spontaneous contained selenium also can not be run off; The toad that sloughs off behind the clothing becomes toad together to raise with other, after 14～16 days, carries out the collection of toad clothing again;
(5), dry, packing: the toad clothing is taken out behind the natural air drying in 35～45 ℃ baking oven continuous drying to water content≤5%, with dividing the bag of packing into behind the disinfection by ultraviolet light, selenium-rich toad-ecdysis.
The concrete weight proportion of described manufactured feed is: feed of animal origin 40～50%, energy feed 10～20%, all the other are vegetable feed.
Described feed of animal origin is one or more in yellow mealworm powder, goose powdered egg, fish meal, the earthworm powder; Energy feed is vitamin and/or growth hormone; Vegetable feed is one or more in dregs of beans, soya-bean cake, corn, wheat bran and the plant leaf powder.
Beneficial effect of the present invention is: the toadlet of toad in the breeding process that becomes toad, added rich selenium bait, the growing environment of rich selenium is provided, make toad self be rich in selenium, and in sloughing off the clothing process, guaranteed not running off of selenium, thereby the toad clothing selenium content height of gathering, improved the medicinal and function value of toad clothing significantly, and the selenium element combines with protein and polysaccharide organic and is converted into Biological Selenium, thereby eliminated chemical selenium (as sodium selenite) toxicity of human body and stomach are stimulated, make the selenium utilization that can more efficient, more safely be absorbed by the body.
Embodiment
Below the invention will be further described.
Selenium-rich toad-ecdysis collecting method of the present invention comprises toad breed stage and toad clothing acquisition phase:
Toad is cultured the stage:
(1), the tadpole phase: inject the water purification behind the Exposure to Sunlight aeration in the tadpole pond, water temperature is 18～24 ℃, the depth of water 20～40cm, with unhurried current water for well; The breeding density of tadpole in the tadpole pond, incipient preceding ten days, breeding density was every square metre of water surface 1000～1500 tails; Ten to 20 days, be every square metre of water surface 600～800 tails; 20 to 30 days, be every square metre of water surface 450～550 tails; In the tadpole pond, cultivate the high algae of selenium content such as diatom, Chlorococcum, chrysophyceae; Implant some pasture and water simultaneously,, account for about half of the water surface, perch and play the effect of sunshade for tadpole as weter hyacinth, duckweed, hornwort etc.; Add high yellow mealworm powder and the goose powdered egg (or living goose egg) of selenium content after 5～7 days, feeding volume is 7%～10% of a tadpole body weight.
(2), the toadlet phase: after about 30 days, tadpole becomes toadlet; The toadlet pond is Tu Chi (or the pond transformation forms), the border situation can be with water sterilization factually, also can put the solid carbon dioxide sterilization, after treating that toxicity disappears, inject the water of 20～40cm behind the Exposure to Sunlight aeration, the water that preferably flows slowly is to increase the water body dissolved oxygen amount, keep water quality pure and fresh, and establish anti-divider wall or the protection network of escaping, preventing the string pond.Toadlet except that the natural living bait of ingesting, the selenium content of also throwing something and feeding high as living baits such as tussah, yellow mealworm, fly maggot, earthworms, day the living bait amount of throwing something and feeding be 10～15% of toadlet body weight; And, the manufactured feed of also throwing something and feeding, day the manufactured feed amount of throwing something and feeding be 7～10% of toadlet body weight, feed every day 2 times, the time is the morning 8～9 and afternoon 5～6 points.In addition, also throw something and feed to cooperate and raise bait, the manufactured feed amount of throwing something and feeding day is 7～10% of a toadlet body weight; Described manufactured feed mainly is made up of feed of animal origin, vegetable feed and energy feed, divide meter by weight percentage: animal feeds such as yellow mealworm powder, goose powdered egg, fish meal, earthworm powder account for 45% of total amount, vegetable feeds such as dregs of beans, soya-bean cake, corn, wheat bran and plant leaf powder account for 45% of total amount, and vitamin and growth hormone energy feed account for 10% of total amount.
(3), become the toad phase: become toad based on land-based activities, become the terrestrial soil in toad pond to press 0.5kg/hm
2Use rich selenium fertilizer, and plantation crops or vegetables, produce selenium-enriched crop and grow rich selenium bait, build awning simultaneously, light trap is installed, the brick bits clitter that some multiple holes are set is perched for toad, also equipment of sprinkler irrigation will be installed, for toad create one quietly, plants burst out in profusion and moist terrestrial environment.Become the rich selenium bait that crops grow in the toad predation plant, the assorted worm of natural world that light trap is lured, and the yellow mealworm of the artificial culture of throwing something and feeding and fly maggot, a day injected volume is into 10～15% of toad body weight.
Toad clothing acquisition phase:
(1), catch: catch the many adult toads of back stain, individuality is more than 100g;
(2), clean: toad is placed service sink, extremely clean with pollution-free neutral fresh water buffering toad;
(3), excite mucous membrane: toad is placed in the container of dry cleansing, not feeding, do not spray water, it is moistening by drying toad secretion mucous membrane to epidermis to be kept, and needs 48 hours approximately, the toad clothing selenium content content height under sloughing off like this;
(4), slough off clothing: toad is placed slough off the clothing pond, inject pollution-free neutral fresh water in the pond, 30 centimetres of the depth of waters add the Se-enriched yeast that Se content reaches 1000～2500mg/kg in the water, and the weight ratio of water and Se-enriched yeast is 30: 1; Toad sloughed off clothing in about about 3 days sloughing off the clothing pond and since during drink be rich selenium water, its spontaneous contained selenium also can not run off simultaneously; Slough off toad behind the clothing and put into in the toad pond and become toad together to raise, after 14～16 days, carry out the collection of toad clothing again with other;
(5), dry, packing: behind toad clothing taking-up natural air drying, continuous drying with dividing the bag of packing into behind the disinfection by ultraviolet light, gets selenium-rich toad-ecdysis to water content≤5% in 40 ℃ baking oven.
The present invention gathers the method for selenium-rich toad-ecdysis, improved the medical value of toad clothing Se content and toad clothing effectively, and the selenium element combines with protein and polysaccharide organic and is converted into Biological Selenium, thereby eliminated chemical selenium (as sodium selenite) toxicity of human body and stomach are stimulated, make the selenium utilization that can more efficient, more safely be absorbed by the body.
Selenium-rich toad-ecdysis and common toad clothing physical and chemical index and microbiological indicator are measured
| Project | Common toad clothing ultra micro powder | Selenium-rich toad-ecdysis ultra micro powder |
| Plumbous (in Pb), mg/kg | ＜0.5 | ＜0.5 |
| Inorganic arsenic (in As), mg/kg | ＜0.5 | ＜0.5 |
| Methyl mercury, mg/kg | ＜0.5 | ＜0.5 |
| Aluminium (in Al), mg/kg | Do not detect | Do not detect |
| Total plate count, cfu/g＜ | 10000 | 10000 |
| Escherichia coli, MPN/100g＜ | 30 | 30 |
| Pathogenic bacteria (salmonella, staphylococcus aureus, Shigella, secondary haemolysis type vibrios) | Do not detect | Do not detect |
| Selenium (in Se), mg/kg | 1 | 2.5 |
Claims (3)
1, a kind of collecting method of selenium-rich toad-ecdysis is characterized in that: comprise toad breed stage and toad clothing acquisition phase;
Toad is cultured the stage:
(1), the tadpole phase: at the water purification of tadpole pond injection behind the Exposure to Sunlight aeration, water temperature is 18～24 ℃, the depth of water 20～40cm; Cultivate the high algae of selenium content in the tadpole pond; Implant the pasture and water that account for the water surface 40～60% simultaneously, perch and play the effect of sunshade for tadpole; Add high yellow mealworm powder or the goose powdered egg of selenium content after 5～7 days, or living goose egg, feeding volume is 7%～10% of a tadpole body weight;
(2), the toadlet phase: the toadlet pond is that Tu Chi or pond transformation form, and after carrying out disinfection and treating that toxicity disappears, injects the water of 20～40cm behind the Exposure to Sunlight aeration; The periphery in toadlet pond is provided with anti-divider wall or the protection network of escaping, preventing the string pond; Cultivate the high algae of selenium content in the toadlet pond, toadlet except that the natural living bait of ingesting, the high living bait of selenium content of also throwing something and feeding, the living bait amount of throwing something and feeding day is 10～15% of a toadlet body weight; And, the manufactured feed of also throwing something and feeding, the manufactured feed amount of throwing something and feeding day is 7～10% of a toadlet body weight;
(3), become the toad phase: become toad based on land-based activities, terrestrial soil is by 0.4～0.6kg/hm
2Use rich selenium fertilizer, and plantation crops or vegetables, produce selenium-enriched crop and grow rich selenium bait; Build awning simultaneously, light trap is installed, the brick bits clitter that multiple hole is set is perched for toad, and equipment of sprinkler irrigation also is installed, for toad provide one quietly, plants burst out in profusion and moist terrestrial environment; The assorted worm of natural world that rich selenium bait that crops grow in the one-tenth toad predation plant and light trap are lured, and the yellow mealworm of the artificial culture of throwing something and feeding and fly maggot, injected volume is into 10～15% of toad body weight;
Toad clothing acquisition phase:
(1), catches: catch many, the individual adult toads more than 100g of back stain;
(2), clean: toad is placed service sink, extremely clean with pollution-free neutral fresh water buffering toad;
(3), excite mucous membrane: toad is placed in the container of dry cleansing, not feeding, do not spray water, it is moistening by drying toad secretion mucous membrane to epidermis to be kept, 40～50 hours, the toad clothing selenium content content height under sloughing off like this;
(4), slough off clothing: toad is placed slough off the clothing pond, inject pollution-free neutral fresh water in the pond, 25～35 centimetres of the depth of waters add Se-enriched yeast in the water, and the weight ratio of water and Se-enriched yeast is 25～35: 1; Toad sloughs off clothing after 40～50 hours in sloughing off the clothing pond and since during drink for rich selenium water, its spontaneous contained selenium also can not be run off; The toad that sloughs off behind the clothing becomes toad together to raise with other, after 14～16 days, carries out the collection of toad clothing again;
(5), dry, packing: the toad clothing is taken out behind the natural air drying in 35～45 ℃ baking oven continuous drying to water content≤5%, with dividing the bag of packing into behind the disinfection by ultraviolet light, selenium-rich toad-ecdysis.
2, the collecting method of a kind of selenium-rich toad-ecdysis according to claim 1 is characterized in that: the concrete weight proportion of described manufactured feed is: feed of animal origin 40～50%, energy feed 10～20%, all the other are vegetable feed.
3, the collecting method of a kind of selenium-rich toad-ecdysis according to claim 2 is characterized in that: described feed of animal origin is one or more in yellow mealworm powder, goose powdered egg, fish meal, the earthworm powder; Energy feed is vitamin and/or growth hormone; Vegetable feed is one or more in dregs of beans, soya-bean cake, corn, wheat bran and the plant leaf powder.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2009100246941A CN101491231A (en) | 2009-02-26 | 2009-02-26 | Collection method of selenium-rich toad-ecdysis |
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| CNA2009100246941A CN101491231A (en) | 2009-02-26 | 2009-02-26 | Collection method of selenium-rich toad-ecdysis |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948638A (en) * | 2012-10-11 | 2013-03-06 | 石台县中昱棘胸蛙特种养殖合作社 | Feed for raising stone frog |
| CN104982384A (en) * | 2015-07-15 | 2015-10-21 | 吉林市昌邑区永发蟾蜍养殖专业合作社 | Toad cortex artificial collection method and toad cortex separator |
| CN105815270A (en) * | 2016-03-18 | 2016-08-03 | 吉林市昌邑区永发蟾蜍养殖专业合作社 | Selenium-enriched toad culture method |
| CN107980710A (en) * | 2016-10-26 | 2018-05-04 | 张伟 | Six Steps are collected in a kind of toad decortication |
| CN109258566A (en) * | 2018-10-10 | 2019-01-25 | 安徽省农业科学院水产研究所 | A method of improving artificial breeding toad dried venom of toads potency |
| CN113455470A (en) * | 2021-08-27 | 2021-10-01 | 上海瀚岳药源生物科技发展有限公司 | Method for artificially culturing toad feeding insects |
-
2009
- 2009-02-26 CN CNA2009100246941A patent/CN101491231A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948638A (en) * | 2012-10-11 | 2013-03-06 | 石台县中昱棘胸蛙特种养殖合作社 | Feed for raising stone frog |
| CN104982384A (en) * | 2015-07-15 | 2015-10-21 | 吉林市昌邑区永发蟾蜍养殖专业合作社 | Toad cortex artificial collection method and toad cortex separator |
| CN105815270A (en) * | 2016-03-18 | 2016-08-03 | 吉林市昌邑区永发蟾蜍养殖专业合作社 | Selenium-enriched toad culture method |
| CN105815270B (en) * | 2016-03-18 | 2018-08-07 | 吉林市昌邑区永发蟾蜍养殖专业合作社 | The cultural method of selenium-rich toad |
| CN107980710A (en) * | 2016-10-26 | 2018-05-04 | 张伟 | Six Steps are collected in a kind of toad decortication |
| CN109258566A (en) * | 2018-10-10 | 2019-01-25 | 安徽省农业科学院水产研究所 | A method of improving artificial breeding toad dried venom of toads potency |
| CN113455470A (en) * | 2021-08-27 | 2021-10-01 | 上海瀚岳药源生物科技发展有限公司 | Method for artificially culturing toad feeding insects |
| CN113455470B (en) * | 2021-08-27 | 2022-03-08 | 上海瀚岳药源生物科技发展有限公司 | Method for artificially culturing toad feeding insects |
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