CN113383868B

CN113383868B - Pet polypeptide nutrient solution and preparation method and application thereof

Info

Publication number: CN113383868B
Application number: CN202110711499.7A
Authority: CN
Inventors: 刘威; 郭维科; 郭维学; 张慧丽
Original assignee: Shanghai Chuanwei Biotechnology Co ltd
Current assignee: Shanghai Chuanwei Biotechnology Co ltd
Priority date: 2021-06-25
Filing date: 2021-06-25
Publication date: 2022-11-18
Anticipated expiration: 2041-06-25
Also published as: CN113383868A

Abstract

The invention relates to a pet polypeptide nutrient solution and a preparation method and application thereof. The specific combination mode is added into the product related to the invention, so that the bitter taste presented by the bitter peptide can be effectively reduced, compared with other taste masking agent components or the single component, the combination mode can more obviously improve the palatability of the product, and pets show more obvious preference.

Description

Pet polypeptide nutrient solution and preparation method and application thereof

Technical Field

The invention belongs to the technical field of pet foods, particularly relates to a pet polypeptide nutrient solution and a preparation method and application thereof, and particularly relates to a pet polypeptide nutrient solution which is good in palatability, high in digestibility, balanced in nutrition and in accordance with the national staple food standard of dogs and cats, and a preparation method and application thereof.

Background

With the continuous development of the pet market in China, the health consciousness of dog and cat pets is continuously enhanced, the formula and nutrition of pet food are paid more attention, the demand of middle and high-end pet food is vigorous, and the liquid food becomes a bright spot in nearly two years and has the advantages of supplementing water and being convenient to digest and absorb. The liquid pet food in the current market mainly comprises dairy products such as cow milk, goat milk and the like and meat pulp products, wherein the dairy products generally contain lactose and are not suitable for feeding pet animals with lactose intolerance; the applicable scenes of the meat pulp products also have limitations, the meat pulp products are not friendly to pets which have difficulty in digestion and absorption in the postoperative recovery period and other scenes, and the palatability is poor because the bitter peptides have certain bitter taste.

In order to solve the limitation of the current pet liquid food market and enrich the product form, the invention develops the pet polypeptide nutrient solution which has good palatability, high digestibility and balanced nutrition, meets the national staple food standard of dogs and cats and is suitable for dogs and cats in various stages and various health states, and the preparation method and the application thereof.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide the pet polypeptide nutrient solution and the preparation method and the application thereof, and particularly provides the pet polypeptide nutrient solution which has good palatability, high digestibility and balanced nutrition and meets the national staple food standard of dogs and cats, and the preparation method and the application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the invention provides a pet polypeptide nutrient solution which is prepared from raw materials including livestock and poultry meat, flavor enzyme, taurine, glycine, glutamic acid, animal fat, natural nutritional supplement, natural food additive and water.

The product related to the invention is added with the specific combination mode, the bitter taste presented by the bitter peptide can be effectively reduced, compared with other taste masking components or the single components, the combination mode can more obviously improve the palatability of the product, and pets show more obvious preference.

"palatability" as referred to herein refers to the relative preference of a pet for one of a food or food composition over another, palatability referring to the overall willingness of an animal to consume a food. Palatability also refers to the ability of the food to be consumed to satisfy the animal, advantageously but not necessarily. Whenever an animal, for example, one of two or more foods, exhibits a preference, the preferred food is more "palatable" and has "enhanced palatability. The relative palatability of one food product compared to other food products may be measured, for example, by the relative consumption of the food products, as determined, for example, in side-by-side, freely-selected comparisons. It may be advantageously determined by a standard test protocol, such as the test known as the "two bowl test", in which the animal has equal chance of approaching both compositions. Such preferences may be derived from any animal perception, but are typically associated with, inter alia, taste, aroma, flavor, texture, odor, or mouthfeel.

Preferably, the raw materials for preparing the pet polypeptide nutrient solution comprise, by mass, 11-63 parts of livestock and poultry meat, 0.1-1 part of flavor enzyme, 0.1-0.9 part of taurine, 0.1-0.5 part of glycine, 0.1-0.5 part of glutamic acid, 1-3 parts of animal fat, 0.2-1.5 parts of natural nutrient supplement, 1-7 parts of natural food additive and 27-77 parts of water.

When the raw materials for preparing the pet polypeptide nutrient solution, particularly taurine, glycine and glutamic acid, are matched according to the specific mass ratio, the prepared product has better palatability.

The parts by mass of the livestock and poultry meat can be 11 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts, 63 parts and the like.

The mass parts of the flavor enzyme can be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like.

The taurine may be in an amount of 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, etc.

The glycine may be present in an amount of 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, etc.

The glutamic acid can be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part and the like in parts by mass.

The animal fat can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts and the like by mass.

The natural nutrient supplement can be 0.2 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 1.0 part, 1.2 parts, 1.4 parts, 1.5 parts and the like in parts by mass.

The natural food additive can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts and the like in parts by mass.

The water may be 27 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 77 parts, or the like by mass.

Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.

More preferably, the raw materials for preparing the pet polypeptide nutrient solution comprise, by mass, 40-63 parts of livestock and poultry meat, 0.3-0.7 part of flavor enzyme, 0.7-0.9 part of taurine, 0.1-0.3 part of glycine, 0.1-0.3 part of glutamic acid, 1-3 parts of animal fat, 0.2-1.5 parts of natural nutrient supplement, 3-5 parts of natural food additive and 30-50 parts of water.

Preferably, the raw materials for preparing the pet polypeptide nutrient solution further comprise, by mass, 0.1-1 part of papain, such as 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like, and other specific values in the numerical range can be selected, which is not described herein again.

In a preferred embodiment of the present invention, papain is added to the raw materials to participate in the enzymatic hydrolysis of the product together with flavor enzymes, and both of them have unexpected synergistic effect in improving the digestibility of the product.

Preferably, the enzyme activity of the flavor enzyme is 20000-50000IU/g, such as 20000IU/g, 25000IU/g, 30000IU/g, 35000IU/g, 40000IU/g, 45000IU/g, 50000IU/g and the like, and other specific points in the numerical range can be selected, so that the description is omitted.

Preferably, the enzyme activity of the papain is 20000-50000IU/g, such as 20000IU/g, 25000IU/g, 30000IU/g, 35000IU/g, 40000IU/g, 45000IU/g, 50000IU/g and the like, and other specific point values in the numerical value range can be selected, so that the details are not repeated.

Preferably, the natural nutritional supplement comprises any one or a combination of at least two of sodium alginate, calcium carbonate, inulin, yucca extract, yak bone powder, rosemary, bovine colostrum or wheat germ powder;

the combination of at least two of the above components, such as the combination of sodium alginate and calcium carbonate, the combination of inulin and yucca extract, the combination of rosemary and bovine colostrum, and the like, can be selected in any combination mode, and are not described in detail herein.

Preferably, the natural food additive comprises any one of sodium chloride, sorbitol, fructooligosaccharide, nisin, or epsilon-polylysine, or a combination of at least two thereof.

The combination of at least two of the above compounds, such as the combination of sodium chloride and sorbitol, the combination of fructooligosaccharide and nisin, the combination of nisin and epsilon-polylysine, and the like, can be selected in any combination manner, and will not be described in detail herein.

Preferably, the animal fat comprises any one of chicken oil, beef tallow, mutton fat or fish oil or the combination of at least two of the chicken oil, the beef tallow, the mutton fat or the fish oil; the combination of at least two of the above-mentioned components, such as the combination of chicken fat and beef tallow, the combination of mutton fat and fish oil, etc., can be selected in any other combination manner, and thus, the details are not repeated herein.

Preferably, the livestock and poultry meat comprises any one of pork, beef, mutton, chicken, duck meat or goose meat or a combination of at least two of the pork, the beef, the mutton, the chicken, the duck meat or the goose meat; the combination of at least two of the above components, such as pork and beef combination, beef and mutton combination, chicken and duck meat combination, and the like, any other combination mode can be selected, and the details are not repeated herein.

In a second aspect, the present invention provides a method for preparing a polypeptide pet nutritional solution as described in the first aspect, the method comprising:

(1) Mixing livestock and poultry meat with water, mincing into meat paste, and steaming and curing;

(2) Mixing the product obtained in the step (1) with flavor enzyme, taurine, glycine and glutamic acid, carrying out enzymolysis, and then carrying out enzyme deactivation treatment to obtain an enzymolysis solution;

(3) And (3) filtering the enzymolysis liquid obtained in the step (2), mixing the filtrate with animal fat, natural nutrient supplements and natural food additives, homogenizing and emulsifying to obtain the pet polypeptide nutrient solution.

The preparation process of the pet polypeptide nutrient solution is simple and easy to operate, is very suitable for industrial scale-up production, and has obvious practical value.

Preferably, the cooking and aging in step (1) is performed at 70-90 ℃ (e.g. 70 ℃, 75 ℃, 80 ℃, 85 ℃, 90 ℃ and the like) for 20-40min (e.g. 20min, 25min, 30min, 35min, 40min and the like), and other specific values in the above numerical range can be selected, and are not repeated herein.

Preferably, the enzymolysis in step (2) is performed at 50-60 ℃ (e.g., 50 ℃, 52 ℃, 55 ℃, 57 ℃, 60 ℃, etc.) for 1.5-3.5h (e.g., 1.5h, 2.0h, 2.5h, 3.0h, 3.5h, etc.), and other specific values within the above numerical range can be selected, which is not described herein again.

Preferably, the enzyme deactivation treatment in step (2) is performed at 85-95 ℃ (e.g., 85 ℃, 87 ℃, 90 ℃, 92 ℃, 95 ℃ and the like) for 10-30min (e.g., 10min, 15min, 20min, 25min, 30min and the like), and other specific values within the above numerical range can be selected, which is not described herein again.

Preferably, the filtering in step (3) is performed by filtering through a 50-70 mesh (e.g., 50 mesh, 55 mesh, 60 mesh, 65 mesh, 70 mesh, etc.) screen, and other specific values in the numerical range can be selected, which are not described in detail herein.

Preferably, the pet polypeptide nutrient solution prepared in the step (3) is sterilized.

In a third aspect, the invention provides a use of the pet polypeptide nutrient solution of the first aspect in preparing a pet feed.

Compared with the prior art, the invention has the following beneficial effects:

the specific combination mode is added into the product related to the invention, so that the bitter taste presented by the bitter peptide can be effectively reduced, compared with other taste masking agent components or the single component, the combination mode can more obviously improve the palatability of the product, and pets show more obvious preference. In a preferred embodiment of the present invention, papain is added to the raw materials to participate in the enzymatic hydrolysis of the product together with flavor enzymes, and both have unexpected synergistic effect in improving the digestibility of the product.

Detailed Description

To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

The flavor enzymes referred to in the following examples and comparative examples were purchased from Nanning Pombo bioengineering, inc. under the model number PBF-02-01; papain is purchased from Nanning Pombo bioengineering Co., ltd, and is PBD-01-01; all of the livestock meats of the examples and comparative examples were used after a bone-removing pretreatment.

Example 1

The embodiment provides a pet polypeptide nutrient solution, which comprises the following preparation raw materials in parts by mass: 30 parts of chicken breast meat, 20 parts of beef tendon meat, 0.5 part of flavor enzyme (enzyme activity 40000 IU/g), 0.8 part of taurine, 0.2 part of glycine, 0.2 part of glutamic acid, 1 part of chicken fat, 0.3 part of sodium alginate, 0.4 part of calcium carbonate, 0.15 part of sodium chloride, 4 parts of sorbitol and 42 parts of water. The preparation method comprises the following steps:

(1) Mixing chicken breast meat, beef tendon meat and water, mincing into meat paste, and cooking at 80 deg.C for 30min;

(2) Mixing the product obtained in the step (1) with flavor enzyme, taurine, glycine and glutamic acid, performing enzymolysis at 55 ℃ for 2.5h, and performing enzyme deactivation treatment at 90 ℃ for 20min to obtain an enzymolysis solution;

(3) And (3) filtering the enzymolysis liquid obtained in the step (2) by using a 60-mesh screen, mixing the filtrate with chicken fat, sodium alginate, calcium carbonate, sodium chloride and sorbitol, homogenizing and emulsifying to obtain the pet polypeptide nutrient solution, and sterilizing.

Example 2

The embodiment provides a pet polypeptide nutrient solution, which comprises the following preparation raw materials in parts by mass: 20 parts of chicken breast meat, 20 parts of sirloin meat, 0.3 part of flavor enzyme (enzyme activity 40000 IU/g), 0.7 part of taurine, 0.3 part of glycine, 0.1 part of glutamic acid, 1 part of chicken fat, 0.2 part of sodium alginate, 0.2 part of calcium carbonate, 0.1 part of sodium chloride, 3 parts of sorbitol and 37 parts of water. The preparation method comprises the following steps:

(1) Mixing chicken breast, sirloin and water, mincing into meat paste, steaming and cooking at 70 deg.C for 40min;

(2) Mixing the product obtained in the step (1) with flavor enzyme, taurine, glycine and glutamic acid, carrying out enzymolysis at 50 ℃ for 3.5h, and carrying out enzyme inactivation treatment at 85 ℃ for 30min to obtain an enzymolysis solution;

(3) And (3) filtering the enzymatic hydrolysate obtained in the step (2) by using a 70-mesh screen, mixing the filtrate with chicken fat, sodium alginate, calcium carbonate, sodium chloride and sorbitol, homogenizing and emulsifying to obtain the pet polypeptide nutrient solution, and sterilizing.

Example 3

The embodiment provides a pet polypeptide nutrient solution, which comprises the following preparation raw materials in parts by mass: 30 parts of chicken breast meat, 30 parts of sirloin meat, 0.7 part of flavor enzyme (enzyme activity 40000 IU/g), 0.8 part of taurine, 0.3 part of glycine, 0.3 part of glutamic acid, 3 parts of chicken fat, 0.5 part of sodium alginate, 0.5 part of calcium carbonate, 0.25 part of sodium chloride, 4 parts of sorbitol and 50 parts of water. The preparation method comprises the following steps:

(1) Mixing chicken breast, sirloin and water, mincing into meat paste, steaming and cooking at 90 deg.C for 20min;

(2) Mixing the product obtained in the step (1) with flavor enzyme, taurine, glycine and glutamic acid, carrying out enzymolysis for 1.5h at 60 ℃, and then carrying out enzyme inactivation treatment for 10min at 95 ℃ to obtain an enzymolysis solution;

(3) And (3) filtering the enzymolysis liquid obtained in the step (2) by using a 50-mesh screen, mixing the filtrate with chicken fat, sodium alginate, calcium carbonate, sodium chloride and sorbitol, homogenizing and emulsifying to obtain the pet polypeptide nutrient solution, and sterilizing.

Example 4

This example provides a pet polypeptide nutritional solution, which is prepared from the raw materials different from example 1 only in that 0.5 part of flavor enzyme is replaced by 0.3 part of flavor enzyme and 0.2 part of papain, and the other conditions are kept unchanged. The preparation process was identical to example 1.

Comparative example 1

The comparative example provides a pet polypeptide nutrient solution, and the preparation raw materials of the pet polypeptide nutrient solution are different from those of the example 1 only in that 0.8 part of taurine, 0.2 part of glycine and 0.2 part of glutamic acid are replaced by 1.2 parts of taurine, and other conditions are kept unchanged. The preparation process was identical to example 1.

Comparative example 2

The comparative example provides a pet polypeptide nutrient solution, and the preparation raw materials of the pet polypeptide nutrient solution are different from those in example 1 only in that 0.8 part of taurine, 0.2 part of glycine and 0.2 part of glutamic acid are replaced by 1.2 parts of glycine, and other conditions are kept unchanged. The preparation process was identical to example 1.

Comparative example 3

The comparative example provides a pet polypeptide nutrient solution, and the preparation raw materials of the pet polypeptide nutrient solution are only different from those of example 1 in that 0.8 part of taurine, 0.2 part of glycine and 0.2 part of glutamic acid are replaced by 1.2 parts of glutamic acid, and other conditions are kept unchanged. The preparation process was identical to example 1.

Comparative example 4

The comparative example provides a pet polypeptide nutrient solution, and the preparation raw materials of the pet polypeptide nutrient solution are different from those of example 1 only in that 0.8 part of taurine, 0.2 part of glycine and 0.2 part of glutamic acid are replaced by 0.96 part of taurine and 0.24 part of glycine, and other conditions are kept unchanged. The preparation process was identical to example 1.

Comparative example 5

The comparative example provides a pet polypeptide nutrient solution, and the preparation raw materials of the pet polypeptide nutrient solution are different from those of example 1 only in that 0.8 part of taurine, 0.2 part of glycine and 0.2 part of glutamic acid are replaced by 0.96 part of taurine and 0.24 part of glutamic acid, and other conditions are kept unchanged. The preparation process was identical to example 1.

Comparative example 6

The comparative example provides a pet polypeptide nutrient solution, and the preparation raw materials of the pet polypeptide nutrient solution are different from those in example 1 only in that 0.8 part of taurine, 0.2 part of glycine and 0.2 part of glutamic acid are replaced by 0.6 part of glycine and 0.6 part of glutamic acid, and other conditions are kept unchanged. The preparation process was identical to example 1.

Comparative example 7

The comparative example provides a pet polypeptide nutrient solution, and the preparation raw materials of the pet polypeptide nutrient solution are different from those in example 1 only in that 0.5 part of flavourzyme is replaced by 0.5 part of papain, and other conditions are kept unchanged. The preparation process was identical to example 1.

Test example 1

And (3) nutrient component detection test:

in the test example, the pet polypeptide nutrient solution prepared in the examples 1 to 4 is subjected to nutrient component detection, a single test is performed for three times, an average value is obtained, the average value is compared with the AFFCO standard, whether the standard is reached is judged, and the test results are shown in the following table 1.

TABLE 1

As can be seen from the data in Table 1, the pet polypeptide nutrient solution has rich and balanced nutrition, and the nutritional indexes meet the national staple food standards of dogs and cats.

Test example 2

Palatability evaluation test-two bowl test:

this test example evaluates the relative palatability of pet polypeptide nutritional liquids prepared in example 1 and comparative examples 1-6. Principle of "double bowl" test: the test is based on the following assumptions: the more the food is consumed, the more palatable it is. 60 labrador dogs of 3 months old (with substantially identical body forms and health conditions) growing under the same breeding standard and the same feeding environment are selected and randomly divided into six groups of 10 dogs each, namely, A, B, C, D, E and F, and the products of example 1 in each group are compared with the products of comparative examples 1 to 6 respectively.

All test dogs were kept in the same environment, free-moving, free-drinking, and the same amount of product from example 1 and comparative example 1 (or comparative examples 2-6) was weighed into the same bowl, the amount present in each ration being sufficient for the daily requirements, and the bowl was placed in a separate feeder accessible to the dog. The test was performed for a total of 3 days, once a day, with a time of 40min per test (if one of the two bowls was completely eaten before the end of the test, both bowls were removed and the test was stopped).

The proportions consumed by the individuals at the end of the test were calculated in% for the examples and comparative examples, respectively, and the average of all the individual proportions was calculated. CR _Examples = consumption of example (g) × 100/(consumption of example + comparative example) (g); CR _{Comparative example} Consumption of comparative example (g) × 100/(consumption of example + comparative example) (g). The results are shown in Table 2.

TABLE 2

As can be seen from the data in Table 2, the pet polypeptide nutrient solution provided by the invention can significantly improve the palatability of the product through the matching combination of taurine, glycine and glutamic acid, and compared with the situation that any one component or a single component is absent, the combination mode can significantly improve the palatability of the product, so that the pet shows more obvious preference.

Test example 3

Digestibility assessment test-stool score:

stool scoring was according to the stool scoring system of the wehao @ research center. Stool scores are according to table 3:

TABLE 3

25 3-month-old English kittens which grow under the same breeding standard and the same feeding environment are selected (the body form and the health condition are basically consistent), and are randomly divided into five groups of A, B, C, D and E, and each group comprises 5 cats. All test cats were kept in the same environment, freely moved, freely drunk water, and fed regularly three times a day. Group a was fed the product of example 1, group B was fed the product of example 2, group C was fed the product of example 3, group D was fed the product of example 4, and group E was fed the product of comparative example 7.

Taking the rest of the diet away based on the free intake of 15 min. The body weight was weighed every two days, and the fecal status of the test cats was observed daily during the test period and recorded. Stool scoring was performed for the test period of 60 days, at 20, 40, and 60 days, respectively, according to the stool scoring system of the wehao @ research center, and the results showed average values, as shown in table 4.

TABLE 4

Group of	20 days	40 days	60 days
				A	2.5	3	3
B	3	2.5	3
				C	2.5	3	3
D	2.5	2.5	2.5
				E	3.5	3.5	3

From the data in table 4, it can be seen that: the pet polypeptide nutrient solution provided by the invention can be beneficial to digestion and absorption of pets, so that the excrement condition is obviously improved, and the gastrointestinal health is improved.

The applicant states that the present invention is illustrated by the above examples to describe a pet polypeptide nutritional liquid of the present invention, and a preparation method and application thereof, but the present invention is not limited to the above examples, i.e. it does not mean that the present invention must be implemented by the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.

It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.

Claims

1. The pet polypeptide nutrient solution is characterized in that raw materials for preparing the pet polypeptide nutrient solution comprise, by mass, 11-63 parts of livestock and poultry meat, 0.1-1 part of flavor enzyme, 0.1-0.9 part of taurine, 0.1-0.5 part of glycine, 0.1-0.5 part of glutamic acid, 1-3 parts of animal fat, 0.2-1.5 parts of natural nutrient supplement, 1-7 parts of natural food additive and 27-77 parts of water;

the pet polypeptide nutrient solution is prepared by the method comprising the following steps:

(3) Filtering the enzymolysis liquid obtained in the step (2), mixing the filtrate with animal fat, natural nutrient supplements and natural food additives, homogenizing and emulsifying to obtain the pet polypeptide nutrient solution;

the enzyme activity of the flavor enzyme is 20000-50000IU/g;

the natural nutrient supplement comprises any one or the combination of at least two of sodium alginate, calcium carbonate, inulin, yucca extract, yak bone powder, rosemary, bovine colostrum or wheat germ powder;

the natural food additive comprises any one or combination of at least two of sodium chloride, sorbitol, fructooligosaccharide, nisin or epsilon-polylysine;

the animal fat comprises any one or the combination of at least two of chicken fat, beef tallow, mutton fat or fish oil;

the livestock and poultry meat comprises any one or combination of at least two of pork, beef, mutton, chicken, duck meat or goose meat;

carrying out enzymolysis at 50-60 ℃ for 1.5-3.5h;

and (3) performing enzyme deactivation treatment at 85-95 ℃ for 10-30min.

2. The pet polypeptide nutrient solution as claimed in claim 1, wherein the raw materials for preparing the pet polypeptide nutrient solution comprise, by mass, 40-63 parts of livestock and poultry meat, 0.3-0.7 part of flavor enzyme, 0.7-0.9 part of taurine, 0.1-0.3 part of glycine, 0.1-0.3 part of glutamic acid, 1-3 parts of animal fat, 0.2-1.5 parts of natural nutrient supplement, 3-5 parts of natural food additive and 30-50 parts of water.

3. A method for preparing pet polypeptide nutritional liquid as claimed in claim 1 or 2, wherein the cooking and ripening in step (1) is performed at 70-90 ℃ for 20-40min.

4. The method for preparing polypeptide pet nutritional solution according to claim 3, wherein the filtering in step (3) is performed by filtering through a 50-70 mesh screen.

5. The method for preparing a pet polypeptide nutrient solution as claimed in claim 3, wherein the pet polypeptide nutrient solution prepared in the step (3) is sterilized.

6. Use of a pet polypeptide nutritional solution according to claim 1 or 2 in the preparation of a pet feed.