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CN112450430A - Preparation method of germinated brown rice jujube juice enzyme powder/tablet rich in natural digestive enzymes - Google Patents

Preparation method of germinated brown rice jujube juice enzyme powder/tablet rich in natural digestive enzymes Download PDF

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Publication number
CN112450430A
CN112450430A CN202011485661.XA CN202011485661A CN112450430A CN 112450430 A CN112450430 A CN 112450430A CN 202011485661 A CN202011485661 A CN 202011485661A CN 112450430 A CN112450430 A CN 112450430A
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brown rice
germinated brown
jujube juice
fermentation
digestive enzymes
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江安娜
刁洋洲
詹志春
吴启涛
李若维
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Wuhan Bosen Health Technology Co ltd
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Wuhan Bosen Health Technology Co ltd
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Abstract

The invention belongs to the technical field of food processing, and particularly relates to a preparation method of germinated brown rice jujube juice enzyme powder rich in natural digestive enzymes, which comprises the following steps: s100, grinding germinated brown rice into pulp, pumping the pulp into a fermentation tank, inoculating the cultured aspergillus niger strains, and culturing for 24-72 hours at 28-32 ℃ to obtain a first fermentation solution; s200, cleaning, peeling and mixing papaya and pineapple, pulping, putting into a fermentation tank, adding jujube juice, and naturally fermenting at normal temperature for 30-60 days to obtain a second fermentation liquid; s300, mixing the first fermentation liquid and the second fermentation liquid, then pumping the mixture into a fermentation tank, inoculating acetic acid bacteria and saccharomycetes, and culturing at 28-30 ℃ for 12-24 hours to obtain mixed fermentation liquid; s400, filtering the mixed fermentation liquor, adding a carrier, and performing spray drying to obtain germinated brown rice jujube juice enzyme powder; the invention also provides a preparation method of the germinated brown rice jujube juice ferment tablet, which is to uniformly mix the germinated brown rice jujube juice ferment powder and auxiliary materials and tablet the mixture. The invention enriches and preserves natural digestive enzyme in the product through raw material selection and process design.

Description

Preparation method of germinated brown rice jujube juice enzyme powder/tablet rich in natural digestive enzymes
Technical Field
The invention belongs to the technical field of food processing, and particularly relates to a preparation method of germinated brown rice jujube juice enzyme powder/tablet rich in natural digestive enzymes.
Background
Digestive enzymes are substances contained in digestive juices secreted from digestive organs of the human body, and are proteins. The main function of digestive enzymes is to break down food into small molecules that can be absorbed by the human body. However, due to various factors such as aging, mental stress, unhealthy lifestyle and environmental pollution, the secretion of digestive enzymes in the body is insufficient, so that it is necessary to supplement the digestive enzymes in our daily diet, but on the one hand, the content of enzymes in the food taken daily is low, and on the other hand, the loss of these enzymes is increased by our cooking method.
The brown rice is a caryopsis of rice grains with rice husks of outer protective sheaths removed from the rice husks and intact inner protective sheaths (pericarp, seed coat and nucellar layer). About 64% of the nutrients in brown rice are accumulated in the bran layer and germ, such as protein, fat, minerals, vitamins, cellulose, etc.
The germinated brown rice is substantially activated as brown rice. The brown rice sprouts are living bodies having vigorous vitality, and contain a large amount of enzymes such as alpha-amylase, beta-amylase, R-enzyme, beta-glucanase, pentosan converting enzyme, maltase, cellulase, hemicellulase, protease, nuclease, lipase, phospholipase, phytase, etc. in a bound state, and are stored in the embryo and cortex. When the moisture of the brown rice is increased to a certain degree, part of the stored substances are changed into sol; meanwhile, the enzyme is converted from a bound state to a free state to perform enzymolysis, and substances insoluble in water in the grains are converted into substances for the embryo to utilize. Therefore, the brown rice germination is essentially a biochemical reaction process; the germinated brown rice is a multi-enzyme system with activated and released enzyme activity.
The red dates are known as vitamin pills and contain 8 essential amino acids for human bodies, and most of the red dates also contain 2 types of arginine and histidine. Every 100g of dried jujube contains 3.3g of protein and 3.1g of crude fiber, and contains calcium, phosphorus, iron and vitamins; contains cyclic Adenosine Monophosphate (AMP), cyclic Guanosine Monophosphate (GMP) and other bioactive substances beneficial to human health, and has the effects of nourishing, strengthening, resisting aging and delaying aging.
Papaya is a fruit in tropical and subtropical regions and has high nutritive and medicinal values. The fruit juice is rich in saccharides, flavonoids and terpenoids, contains beta-carotene, organic acid, papain and other nutrient substances, has the effects of protecting liver, resisting inflammation, easing pain, resisting tumors, resisting bacteria, resisting oxidation and the like, is a member of fruits used as medicine and food, and is rich in papain.
The pineapple is rich in nutrition, is rich in sugar, protein, fat, vitamin A, B1, B2, C, bromelain, mineral substances, organic acid and the like, and is rich in bromelain.
The existing ferment products have low enzyme activity, and the ferment products rich in digestive enzymes are basically industrial enzyme preparations added from external sources, and have certain insecurity in the aspects of addition amount and sources.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide the preparation method of the germinated brown rice jujube juice enzyme powder/tablet rich in natural digestive enzymes, the natural digestive enzymes are enriched and stored in the product through raw material selection and process design, the problems that the enzyme activity of the existing enzyme products is low or an industrial enzyme preparation needs to be added externally are solved, and the tablet is convenient to carry and eat.
In order to achieve the purpose, the technical scheme of the invention is a preparation method of germinated brown rice jujube juice enzyme powder rich in natural digestive enzymes, which comprises the following steps:
s100, grinding fresh germinated brown rice into pulp, pumping the obtained germinated brown rice pulp into a fermentation tank, inoculating the cultured aspergillus niger strain, and culturing at 28-32 ℃ for 24-72 hours to obtain a first fermentation solution;
s200, cleaning, peeling, mixing and pulping papaya and pineapple, pumping the obtained mixed pulp into a fermentation tank, adding jujube juice into the mixed pulp, uniformly mixing, and naturally fermenting at normal temperature for 30-60d to obtain a second fermentation liquid;
s300, mixing the first fermentation liquid and the second fermentation liquid according to a weight ratio of 1: 5-1: 7 mixing, putting into a fermentation tank, inoculating acetic acid bacteria and yeast, and culturing at 28-30 deg.C for 12-24h to obtain mixed fermentation liquid;
s400, filtering the mixed fermentation liquor, adding a carrier accounting for 20-30% of the weight of the mixed fermentation liquor, and performing spray drying to obtain the germinated brown rice jujube juice enzyme powder.
Further, in step S100, the cultured Aspergillus niger species is inoculated into a fermenter at an inoculum size of 3-5% for culture.
Further, in step S100, Aspergillus niger species are cultured as follows: inoculating the preserved Aspergillus niger strain to sterilized PDA solid culture medium, and performing slant culture at 28-32 deg.C for 24-48 h; then inoculating the aspergillus niger strain cultured on the inclined plane into a liquid culture medium, and carrying out shake culture at the temperature of 28 ℃ and at the speed of 150-; finally, inoculating the Aspergillus niger culture solution cultured by a shaking table into a sterilized rice milk culture medium according to the proportion of 1-2% for culture to obtain a cultured Aspergillus niger strain; wherein the rice milk culture medium is prepared from fresh germinated brown rice and water according to a weight ratio of 1: 8 mixing and grinding the materials to obtain pulp, and sterilizing the pulp at 100 ℃ for 30 min.
Further, in step S200, the ratio by weight of the papaya to the pineapple is 1: 1-2, mixing and pulping, wherein the addition amount of the jujube juice is 3-6% of the weight of the mixed pulp.
Further, in step S300, the total inoculation amount of the acetic acid bacteria and the yeast is 1-2.0%, and the inoculation ratio of the acetic acid bacteria to the yeast is 1.2: 1-2: 1.
further, in step S400, the carrier includes oxidized starch and resistant dextrin, and a weight ratio of the oxidized starch to the resistant dextrin is 3: 2 or 4: 1.
further, in step S400, the spray drying process parameters are as follows: the air inlet temperature is 150-160 ℃, and the air outlet temperature is 60-70 ℃.
The invention also provides a preparation method of the germinated brown rice jujube juice ferment tablet rich in natural digestive enzyme, which comprises the steps of mixing the germinated brown rice jujube juice ferment powder prepared by the preparation method with auxiliary materials to obtain formula powder, uniformly mixing the formula powder and tabletting to obtain the germinated brown rice jujube juice ferment tablet.
Further, the auxiliary materials comprise direct compression D-mannitol, resistant dextrin, microcrystalline cellulose and magnesium stearate; the formula powder comprises the following components in percentage by weight: 60-80% of germinated brown rice jujube juice enzyme powder, 4-10% of resistant dextrin, 10-20% of direct-compression D-mannitol, 5-10% of microcrystalline cellulose and 0.5-1% of magnesium stearate.
Further, during tabletting, the ambient humidity is controlled to be 45-60%, the filling pressure of the tabletting machine is 18-25N, and the filling depth is 8.0-8.4 mm.
Compared with the prior art, the invention has the following beneficial effects:
(1) the germinated brown rice jujube juice enzyme powder/tablet prepared by the invention is rich in natural active enzyme, and the active enzyme contained in the enzyme powder and the enzyme tablet products comprises the following components: protease, amylase, glucoamylase, lipase, cellulase, SOD, etc.;
(2) in order to achieve the purpose of enriching the active enzyme, in the fermentation stage, according to the growth characteristics of different strains, the invention adopts the methods of inoculation and natural fermentation, and aspergillus niger has excellent performance for producing the active enzyme and grows rapidly, so that a large amount of active enzyme can be accumulated in a relatively short time when the germinated brown rice pulp is fermented; in the natural fermentation of the papaya, the pineapple pulp and the red date juice, the red date juice can provide a sufficient natural carbon source, and after long-term natural fermentation, the generated active enzyme is 2-3 times of that of the inoculated fermentation; in addition, the fruit pulp fermentation can also be used for adding specific flavor to the product;
(3) the raw materials and strains selected in the invention not only generate a large amount of active enzyme, but also the germinated brown rice used and the acetic acid bacteria and the microzyme obtained by screening from the black tea fungus can generate a large amount of GABA, gluconic acid, glucaric acid, glucuronic acid and glucaric acid-1, 4-lactone, so that the ferment powder and the ferment tablet products are rich in the functional components, and after entering a human body, the ferment powder and the ferment tablet products mutually promote the effect of the active enzyme, and the immunity of the human body is improved;
(4) the method for obtaining the natural active enzyme is characterized in that the natural active enzyme is gradually enriched in three steps of preparing the fermentation liquor, and then enzyme powder rich in the natural active enzyme is prepared, in addition, the enzyme powder is used as a main raw material for preparing the ferment tablet, and a full-powder direct tabletting process is adopted instead of the traditional wet tabletting process, so that the active enzyme in the enzyme powder is protected to the maximum extent, and the industrial cost is saved to the maximum extent;
(5) in order to smoothly tablet and ensure the product quality of the ferment tablet, the raw and auxiliary materials of the formula need to have lower hygroscopicity, good fluidity and compressibility, so that the three factors are comprehensively considered from the preparation process of the ferment powder to the selection of the raw and auxiliary materials of the formula of the follow-up ferment tablet, and the achievement of the final target of the product is ensured through repeated experiments; therefore, from the design and optimization of the fermentation process to the spray drying of the fermentation liquid to prepare the ferment powder and then to the preparation of the ferment tablet by taking the ferment powder as the main raw material, all process links are closely related and are gradually promoted;
(6) in consideration of smooth powder discharge of spray drying and reduction of hygroscopicity of subsequent enzyme powder and enzyme tablets, the carrier is selected from oxidized starch and resistant dextrin for matching use, and the weight ratio of the oxidized starch to the resistant dextrin is 3: 2 or 4: 1, smooth powder discharging can be ensured, and the hygroscopicity of enzyme powder and enzyme tablets can be reduced, so that the preservation is facilitated;
(7) the enzyme powder and the ferment tablet prepared by the invention meet the requirements of industrial production, have no safety risk and have high utilization rate of raw materials.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The embodiment provides a preparation method of germinated brown rice jujube juice enzyme powder rich in natural digestive enzymes, which comprises the following steps:
s100, taking fresh germinated brown rice (the germination rate reaches more than 80%) and water according to the weight ratio of 1: 1, mixing, grinding into thick liquid, pumping the obtained germinated brown rice pulp into a sterilized closed fermentation tank, inoculating the cultured aspergillus niger into the fermentation tank according to the inoculation amount of 3%, culturing for 24-72h at 28 ℃, and detecting enzyme activity to obtain a first fermentation liquid;
the aspergillus niger strains are cultured in advance according to the following method: inoculating the preserved Aspergillus niger strain to sterilized PDA solid culture medium, and performing slant culture at 28-32 deg.C for 24-48 h; then inoculating the aspergillus niger strain cultured on the inclined plane into a liquid culture medium, and carrying out shake culture at the temperature of 28 ℃ and at the speed of 150-; finally, inoculating the Aspergillus niger culture solution cultured by a shaking table into a sterilized rice milk culture medium according to the proportion of 2% for culturing to obtain a cultured Aspergillus niger strain; the rice milk culture medium is prepared from fresh germinated brown rice and water according to the weight ratio of 1: 8 mixing and grinding the pulp to obtain pulp, and sterilizing the pulp at 100 ℃ for 30 min;
as Aspergillus niger is used as an aerobic microorganism, the growth is rapid, the metabolism is vigorous, and the viability is strong, therefore, the quality of protein and enzyme produced by an Aspergillus niger expression system is high, the yield is high, and the yield of active enzyme in a fermentation system needs to be closely concerned during the fermentation of the mold; detecting enzyme activity in a fermentation system every 4 hours from 24 hours, wherein the enzyme activity comprises amylase, saccharifying enzyme, lipase, acid protease, cellulase and SOD, and according to the detection result, ending the fermentation to obtain a first fermentation solution when the enzyme activity of each enzyme is gradually increased;
s200, selecting high-quality papaya and pineapple, cleaning, peeling, and mixing the papaya and the pineapple according to a weight ratio of 1: 2, mixing and pulping, namely pumping the obtained mixed pulp into a fermentation tank, adding jujube juice into the mixed pulp, wherein the addition amount of the jujube juice is 3% of the weight of the mixed pulp, uniformly mixing, sealing, naturally fermenting at normal temperature for 30-60 days, and detecting enzyme activity to obtain a second fermentation liquid;
in the long-term natural fermentation process, the production of the active enzyme has a process of increasing firstly and then decreasing, so that in the fermentation process, the active enzyme activity in the fermentation liquor needs to be detected by sampling in time according to the fermentation condition, and the detection of the enzyme activity comprises the following steps: amylase, saccharifying enzyme, lipase, protease and SOD, and according to the final detection result, ending the fermentation to obtain a second fermentation liquid when the total increase of the enzyme activities of the above enzymes is gradual;
s300, mixing the first fermentation liquid and the second fermentation liquid according to a weight ratio of 1: 5 mixing the two materials, putting the mixture into a fermentation tank, inoculating acetic acid bacteria and yeast, wherein the total inoculation amount of the acetic acid bacteria and the yeast is 2.0 percent, and the inoculation ratio of the acetic acid bacteria to the yeast is 1.2: culturing at 1 and 30 ℃ for 12h to obtain mixed fermentation liquor;
wherein the acetic acid bacteria and yeast are selected from black tea fungus to obtain high-yield gluconic acid, glucaric acid, glucuronic acid and glucaric acid-1, 4-lactone;
s400, filtering the mixed fermentation liquor by using a plate and frame, adding a carrier accounting for 30% of the weight of the filtrate into the filtrate, wherein the carrier adopts oxidized starch and resistant dextrin according to a weight ratio of 4: 1, matching and using, and carrying out spray drying to obtain the germinated brown rice jujube juice enzyme powder rich in natural digestive enzyme; the technological parameters of spray drying are as follows: the air inlet temperature is 160 ℃ and the air outlet temperature is 60 ℃;
the embodiment also provides a preparation method of the germinated brown rice jujube juice ferment tablet rich in natural digestive enzymes, wherein the germinated brown rice jujube juice ferment powder prepared by the preparation method is mixed with crushed auxiliary materials to obtain formula powder, the formula powder is accurately measured according to the formula proportion, and the formula powder is sieved by a 60-mesh sieve, so that the mixture is more uniform during subsequent mixing; putting the screened formula powder into a mixer, mixing for about 10min, taking out, directly tabletting the mixed formula powder by using a rotary tablet press under the condition that the ambient humidity is 60% to obtain the germinated brown rice jujube juice ferment tablet, wherein the filling pressure of the tablet press is 18-25N, the filling depth is 8.0-8.4mm, the weight of the ferment tablet is controlled to be about 0.6g, and the friability of the obtained ferment tablet finished product is less than 1%;
in order to ensure smooth tabletting and quality of the yeast tablets, the auxiliary materials comprise direct compression D-mannitol, resistant dextrin, microcrystalline cellulose and magnesium stearate, and the formula powder comprises the following components in percentage by weight: 60% of germinated brown rice jujube juice enzyme powder, 9% of resistant dextrin, 20% of direct-compression D-mannitol, 10% of microcrystalline cellulose and 1% of magnesium stearate; pulverizing the raw materials with particle size of more than 60 meshes, controlling the particle size at 80-100 meshes, and mixing.
Example 2
The embodiment provides a preparation method of germinated brown rice jujube juice enzyme powder rich in natural digestive enzymes, which comprises the following steps:
s100, taking fresh germinated brown rice (the germination rate reaches more than 80%) and water according to the weight ratio of 1: 3, mixing and grinding the mixture, pumping the obtained germinated brown rice pulp into a sterilized closed fermentation tank, inoculating the cultured aspergillus niger strains into the fermentation tank according to the inoculation amount of 4%, culturing for 24-72 hours at 28 ℃, and detecting enzyme activity to obtain a first fermentation solution;
the aspergillus niger strains are cultured in advance according to the following method: inoculating the preserved Aspergillus niger strain to sterilized PDA solid culture medium, and performing slant culture at 28-32 deg.C for 24-48 h; then inoculating the aspergillus niger strain cultured on the inclined plane into a liquid culture medium, and carrying out shake culture at the temperature of 28 ℃ and at the speed of 150-; finally, inoculating the Aspergillus niger culture solution cultured by a shaking table into a sterilized rice milk culture medium according to the proportion of 1% for culturing to obtain a cultured Aspergillus niger strain; the rice milk culture medium is prepared from fresh germinated brown rice and water according to the weight ratio of 1: 8 mixing and grinding the pulp to obtain pulp, and sterilizing the pulp at 100 ℃ for 30 min;
as Aspergillus niger is used as an aerobic microorganism, the growth is rapid, the metabolism is vigorous, and the viability is strong, therefore, the quality of protein and enzyme produced by an Aspergillus niger expression system is high, the yield is high, and the yield of active enzyme in a fermentation system needs to be closely concerned during the fermentation of the mold; detecting enzyme activity in a fermentation system every 4 hours from 24 hours, wherein the enzyme activity comprises amylase, saccharifying enzyme, lipase, acid protease, cellulase and SOD, and according to the detection result, ending the fermentation to obtain a first fermentation solution when the enzyme activity of each enzyme is gradually increased;
s200, selecting high-quality papaya and pineapple, cleaning, peeling, and mixing the papaya and the pineapple according to a weight ratio of 1: 1, mixing and pulping, namely pumping the obtained mixed pulp into a fermentation tank, adding jujube juice into the mixed pulp, wherein the addition amount of the jujube juice is 4% of the weight of the mixed pulp, uniformly mixing, and sealing for natural fermentation at normal temperature for 30-60 days to obtain second fermentation liquor;
in the long-term natural fermentation process, the production of the active enzyme has a process of increasing firstly and then decreasing, so that in the fermentation process, the active enzyme activity in the fermentation liquor needs to be detected by sampling in time according to the fermentation condition, and the detection of the enzyme activity comprises the following steps: amylase, saccharifying enzyme, lipase, protease and SOD, and according to the final detection result, ending the fermentation to obtain a second fermentation liquid when the total increase of the enzyme activities of the above enzymes is gradual;
s300, combining the first fermentation liquor and the second fermentation liquor according to the weight ratio of 1: 7, inoculating acetic acid bacteria and yeast into the fermentation tank, wherein the total inoculation amount of the acetic acid bacteria and the yeast is 1.5 percent, and the inoculation ratio of the acetic acid bacteria to the yeast is 2: culturing at 1, 28 deg.C for 16h to obtain mixed fermentation liquid;
wherein the acetic acid bacteria and yeast are selected from black tea fungus to obtain high-yield gluconic acid, glucaric acid, glucuronic acid and glucaric acid-1, 4-lactone;
s400, filtering the mixed fermentation liquor by using a plate frame, adding a carrier accounting for 20% of the weight of the filtrate into the filtrate, wherein the carrier adopts oxidized starch and resistant dextrin according to a weight ratio of 3: 2, matching and using, and carrying out spray drying to obtain the germinated brown rice jujube juice enzyme powder rich in natural digestive enzyme; the technological parameters of spray drying are as follows: the air inlet temperature is 150 ℃ and the air outlet temperature is 70 ℃;
the embodiment also provides a preparation method of the germinated brown rice jujube juice ferment tablet rich in natural digestive enzymes, wherein the germinated brown rice jujube juice ferment powder prepared by the preparation method is mixed with crushed auxiliary materials to obtain formula powder, the formula powder is accurately measured according to the formula proportion, and the formula powder is sieved by a 60-mesh sieve, so that the germinated brown rice jujube juice ferment tablet is more uniform in subsequent mixing; putting the screened formula powder into a mixer, mixing for about 15min, taking out, directly tabletting the mixed formula powder by using a rotary tablet press under the condition that the ambient humidity is 45% to obtain the germinated brown rice jujube juice ferment tablet, wherein the filling pressure of the tablet press is 18-25N, the filling depth is 8.0-8.4mm, the weight of the ferment tablet is controlled to be about 0.6g, and the friability of the obtained ferment tablet finished product is less than 1%;
in order to ensure smooth tabletting and quality of the yeast tablets, the auxiliary materials comprise D-mannitol, resistant dextrin, microcrystalline cellulose and magnesium stearate, and the formula powder comprises the following components in percentage by weight: 80% of germinated brown rice jujube juice enzyme powder, 4.5% of resistant dextrin, 10% of D-mannitol, 5% of microcrystalline cellulose and 0.5% of magnesium stearate; pulverizing the raw materials with particle size of more than 60 meshes, controlling the particle size at 80-100 meshes, and mixing.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. The preparation method of the germinated brown rice jujube juice enzyme powder rich in natural digestive enzymes is characterized by comprising the following steps:
s100, grinding fresh germinated brown rice into pulp, pumping the obtained germinated brown rice pulp into a fermentation tank, inoculating the cultured aspergillus niger strain, and culturing at 28-32 ℃ for 24-72 hours to obtain a first fermentation solution;
s200, cleaning, peeling, mixing and pulping papaya and pineapple, pumping the obtained mixed pulp into a fermentation tank, adding jujube juice into the mixed pulp, uniformly mixing, and naturally fermenting at normal temperature for 30-60d to obtain a second fermentation liquid;
s300, mixing the first fermentation liquid and the second fermentation liquid according to a weight ratio of 1: 5-1: 7 mixing, putting into a fermentation tank, inoculating acetic acid bacteria and yeast, and culturing at 28-30 deg.C for 12-24h to obtain mixed fermentation liquid;
s400, filtering the mixed fermentation liquor, adding a carrier accounting for 20-30% of the weight of the filtrate, and performing spray drying to obtain the germinated brown rice jujube juice enzyme powder.
2. The method for preparing the germinated brown rice jujube juice ferment powder rich in natural digestive enzymes according to claim 1, wherein the method comprises the following steps: in step S100, the cultured Aspergillus niger strains are inoculated into a fermentation tank according to the inoculation amount of 3-5% for culture.
3. The method for preparing the germinated brown rice jujube juice ferment powder rich in natural digestive enzymes according to claim 1, wherein the method comprises the following steps: in step S100, Aspergillus niger strains are cultured as follows: inoculating the preserved Aspergillus niger strain to sterilized PDA solid culture medium, and performing slant culture at 28-32 deg.C for 24-48 h; then inoculating the aspergillus niger strain cultured on the inclined plane into a liquid culture medium, and carrying out shake culture at the temperature of 28 ℃ and at the speed of 150-; finally, inoculating the Aspergillus niger culture solution cultured by a shaking table into a sterilized rice milk culture medium according to the proportion of 1-2% for culture to obtain a cultured Aspergillus niger strain; wherein the rice milk culture medium is prepared from fresh germinated brown rice and water according to a weight ratio of 1: 8 mixing and grinding the materials to obtain pulp, and sterilizing the pulp at 100 ℃ for 30 min.
4. The method for preparing the germinated brown rice jujube juice ferment powder rich in natural digestive enzymes according to claim 1, wherein the method comprises the following steps: in step S200, the papaya and the pineapple are mixed according to a weight ratio of 1: 1-2, mixing and pulping, wherein the addition amount of the jujube juice is 3-6% of the weight of the mixed pulp.
5. The method for preparing the germinated brown rice jujube juice ferment powder rich in natural digestive enzymes according to claim 1, wherein the method comprises the following steps: in step S300, the total inoculation amount of the acetic acid bacteria and the yeast is 1-2.0%, and the inoculation ratio of the acetic acid bacteria to the yeast is 1.2: 1-2: 1.
6. the method for preparing the germinated brown rice jujube juice ferment powder rich in natural digestive enzymes according to claim 1, wherein the method comprises the following steps: in step S400, the carrier includes oxidized starch and resistant dextrin, and the weight ratio of the oxidized starch to the resistant dextrin is 3: 2 or 4: 1.
7. the method for preparing the germinated brown rice jujube juice ferment powder rich in natural digestive enzymes according to claim 1, wherein the method comprises the following steps: in step S400, the spray drying process parameters are: the air inlet temperature is 150-160 ℃, and the air outlet temperature is 60-70 ℃.
8. A preparation method of germinated brown rice jujube juice ferment tablet rich in natural digestive enzyme is characterized in that: mixing the germinated brown rice jujube juice ferment powder prepared according to the preparation method of any one of claims 1 to 7 with auxiliary materials to obtain formula powder, uniformly mixing the formula powder, and tabletting to obtain the germinated brown rice jujube juice ferment tablet.
9. The method for preparing the germinated brown rice jujube juice ferment tablet rich in natural digestive enzymes according to claim 8, wherein the natural digestive enzymes are selected from the group consisting of: the auxiliary materials comprise direct compression D-mannitol, resistant dextrin, microcrystalline cellulose and magnesium stearate; the formula powder comprises the following components in percentage by weight: 60-80% of germinated brown rice jujube juice enzyme powder, 4-10% of resistant dextrin, 10-20% of direct-compression D-mannitol, 5-10% of microcrystalline cellulose and 0.5-1% of magnesium stearate.
10. The method for preparing the germinated brown rice jujube juice ferment tablet rich in natural digestive enzymes according to claim 8, wherein the natural digestive enzymes are selected from the group consisting of: during tabletting, the environment humidity is controlled to be 45-60%, the filling pressure of the tabletting machine is 18-25N, and the filling depth is 8.0-8.4 mm.
CN202011485661.XA 2020-12-16 2020-12-16 Preparation method of germinated brown rice jujube juice enzyme powder/tablet rich in natural digestive enzymes Pending CN112450430A (en)

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