CN112189511A

CN112189511A - Culture medium suitable for artificially domesticating mycelium and fruiting body of antrodia camphorata

Info

Publication number: CN112189511A
Application number: CN202011107171.6A
Authority: CN
Inventors: 王勇凯; 李勇新
Original assignee: Shanghai Huahong Biotechnology Co ltd
Current assignee: Shanghai Huahong Biotechnology Co ltd
Priority date: 2020-10-16
Filing date: 2020-10-16
Publication date: 2021-01-08

Abstract

The invention provides a culture medium suitable for artificially domesticating antrodia cinnamomea mycelia and fruiting bodies, which comprises main materials, auxiliary materials, cane sugar and trace elements, wherein the mass ratio of the main materials to the auxiliary materials is 3:1, and the main materials mainly comprise: camphor wood powder, ganoderma lucidum powder, Chinese yam, astragalus root, liquorice, camphor tree leaves, corncobs and the like. The culture medium suitable for artificially domesticating the antrodia camphorata mycelia and the fruiting bodies, provided by the invention, has the advantages that the raw material components of the culture medium are reasonable in proportion and resources are saved, the antrodia camphorata mycelia can grow on the culture material healthily and quickly, and the problem of slow fungus growth in antrodia camphorata mycelia and fruiting body bag material cultivation is solved.

Description

Culture medium suitable for artificially domesticating mycelium and fruiting body of antrodia camphorata

Technical Field

The invention relates to a culture medium for antrodia cinnamomea mycelia and fruiting bodies, in particular to a culture medium suitable for artificially domesticating the antrodia cinnamomea mycelia and the fruiting bodies.

Background

Antrodia camphorata, also known as Antrodia camphorata, belongs to the order Aphyllophorales, the family Polyporaceae, the family of perennial fungi, and is known by the academic name (Antrodiacamphora), a new species published by the Biochemical kingdom in 1990. The growing area is a mountain forest at an altitude of 450-2000 m in Taiwan mountain areas, and the social events of stealing the camphor trees are still smelled because the stems of the camphor trees grow on the rotten inner wall of the special camphor trees of Taiwan for more than one hundred years or the withered and lodging camphor trees have wet surfaces and are difficult to collect. Antrodia camphorata is regarded as a unique and precious medicinal fungus in Taiwan, has extremely high research and commercial values, is the most expensive wild fungus in Taiwan at present, is called as 'Shenzhi' in hong Kong and is called as 'ruby in forest' in Taiwan folk.

However, the antrodia camphorata is generally a wild plant, and the wild quantity cannot meet the requirements of people, so that the artificial cultivation of the antrodia camphorata is urgent.

Disclosure of Invention

Aiming at the problems pointed out in the background technology, the invention provides a culture medium suitable for artificially domesticating antrodia camphorata mycelia and fruiting bodies, and aims to solve the related technical problems of slow bacteria of an antrodia camphorata mycelium method.

The technical scheme of the invention is realized as follows:

the culture medium suitable for artificially domesticating antrodia cinnamomea mycelia and fruiting bodies is characterized by comprising main materials and auxiliary materials, wherein the mass ratio of the main materials to the auxiliary materials is 3:1, and the main materials comprise the following components in parts by mass: 28-32 parts of camphor wood powder, 18-22 parts of ganoderma lucidum powder, 3-6 parts of Chinese yam, 4-7 parts of astragalus membranaceus, 8-11 parts of liquorice and 9-12 parts of camphor tree leaves.

Preferably, the auxiliary material is a mixture of wheat bran, corn and gypsum, wherein the auxiliary material comprises the following components in parts by weight: 4-7 parts of wheat bran, 8-11 parts of corn, 1-3 parts of gypsum and 1-4 parts of cane sugar.

Preferably, the culture medium further comprises trace elements and sucrose, wherein the sucrose and the trace elements are dissolved by purified water and then mixed with the main material and the auxiliary material to form the culture medium.

Preferably, the trace elements are a mixture of selenium, zinc, iron and manganese, wherein the mass fraction is as follows: 8-10 g of selenium, 9-11 g of zinc, 10-11 g of iron and 8-11 g of manganese.

Preferably, the water content of the culture medium is controlled to be 58% -62%, and the culture medium can be bagged after being subjected to stacking and closed fermentation for 1-3 hours.

Preferably, the packed medium is sterilized at atmospheric pressure for 10 hours.

In conclusion, the beneficial effects of the invention are as follows: the culture medium suitable for artificially domesticating the antrodia camphorata mycelia and the fruiting bodies, provided by the invention, has the advantages that the raw material components of the culture medium are reasonable in proportion and resources are saved, the antrodia camphorata mycelia can grow on the culture material healthily and quickly, and the problem of slow fungus growth in antrodia camphorata mycelia and fruiting body bag material cultivation is solved.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

A culture medium suitable for artificially domesticating antrodia cinnamomea mycelia and fruiting bodies comprises main materials, auxiliary materials, cane sugar and trace elements, wherein the mass ratio of the main materials to the auxiliary materials is 3:1, and the main materials comprise the following components in parts by mass: 30 parts of camphor wood powder, 20 parts of ganoderma lucidum powder, 5 parts of Chinese yam, 5 parts of astragalus membranaceus, 10 parts of liquorice and 10 parts of camphor tree leaves.

The auxiliary materials are a mixture of wheat bran, corn and gypsum, wherein the auxiliary materials comprise, by mass, 6 parts of wheat bran, 10 parts of corn, 2 parts of gypsum and 2 parts of cane sugar.

The trace elements are a mixture of selenium, zinc, iron and manganese, wherein the mixture comprises the following components in parts by mass: 10 g of selenium, 10 g of zinc, 10 g of iron and 10 g of manganese.

By adopting the technical scheme, when the antrodia camphorata is cultured, the method comprises the following operation steps:

s1, proportioning: weighing the crushed mixture, adding water to dissolve sugar and trace elements, and mixing the dissolved sugar and trace elements into the mixture;

s2, fermentation: controlling the water content of the culture medium to be 58% -62%, uniformly stirring, tightly piling for 1-3 hours, and bagging;

s3, bagging: sleeving a plastic primary-secondary ring on the bag, and covering a breathable cover with filter cotton;

s4, sterilization: the culture base material is bagged into a truck and sterilized for 10 hours under normal pressure;

s5, inoculation: inoculating with an internal inoculation process, and transferring to a bed frame of a spawn running culture room;

s6, cultivation: culturing under dark condition at 23-25 deg.c for 40-60 days.

S7, quasi-ecological cultivation: and (3) moving the culture medium into a mushroom-out room when the fungus bags are fully covered with hyphae, providing weak light in the daytime, matching with a quasi-ecological cultivation process, controlling the temperature at 25-28 ℃, controlling the relative humidity at 70% -75%, and properly ventilating.

By adopting the technical scheme, 30-45 days are required from inoculation to generation of a large number of sporocarp.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. The culture medium suitable for artificially domesticating antrodia cinnamomea mycelia and fruiting bodies is characterized by comprising main materials and auxiliary materials, wherein the mass ratio of the main materials to the auxiliary materials is 3:1, and the main materials comprise the following components in parts by mass: 28-32 parts of camphor wood powder, 18-22 parts of ganoderma lucidum powder, 3-6 parts of Chinese yam, 4-7 parts of astragalus membranaceus, 8-11 parts of liquorice and 9-12 parts of camphor tree leaves.

2. The culture medium suitable for artificially acclimating mycelia and fruiting bodies of antrodia camphorata according to claim 1, wherein: the auxiliary material is a mixture of wheat bran, corn and gypsum, wherein the auxiliary material comprises the following components in parts by mass: 4-7 parts of wheat bran, 8-11 parts of corn, 1-3 parts of gypsum and 1-4 parts of cane sugar.

3. The culture medium suitable for artificially acclimating mycelia and fruiting bodies of antrodia camphorata according to claim 1, wherein: the culture medium is characterized by further comprising trace elements and sucrose, wherein the sucrose and the trace elements are dissolved in purified water and then are mixed with the main material and the auxiliary material to form the culture medium.

4. The culture medium suitable for artificially acclimating mycelia and fruiting bodies of antrodia camphorata according to claim 3, wherein: the trace elements are a mixture of selenium, zinc, iron and manganese, wherein the mixture comprises the following components in parts by mass: 8-10 g of selenium, 9-11 g of zinc, 10-11 g of iron and 8-11 g of manganese.

5. The culture medium suitable for artificially acclimating mycelia and fruiting bodies of antrodia camphorata according to claim 3, wherein: controlling the water content of the culture medium to be 58% -62%, and bagging after piling, sealing and fermenting for 1-3 hours.

6. The culture medium suitable for artificially acclimating mycelia and fruiting bodies of antrodia camphorata according to claim 5, wherein: the packed culture medium is sterilized under normal pressure for 10 hours.