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CN101743851A - Method for producing agrocybe aegerita - Google Patents

Method for producing agrocybe aegerita Download PDF

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Publication number
CN101743851A
CN101743851A CN200810233380A CN200810233380A CN101743851A CN 101743851 A CN101743851 A CN 101743851A CN 200810233380 A CN200810233380 A CN 200810233380A CN 200810233380 A CN200810233380 A CN 200810233380A CN 101743851 A CN101743851 A CN 101743851A
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China
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percent
fertilisers
composts
cultivating
producing
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Pending
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CN200810233380A
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Chinese (zh)
Inventor
王涛
陈泽军
祝晓波
周瑞平
游玲
邱芳
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Yibin University
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Yibin University
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Abstract

The invention relates to a method for producing agrocybe aegerita, which comprises the following steps of: adding 3 to 5 percent of lime into fresh concentrated fragrance type liquor waste lees, mixing the lime and the liquor waste lees uniformly, and adjusting the pH value to be 9 to 12; mixing the following components uniformly in percentage by weight: 80 to 95 percent of the waste lees, 1 to 10 percent of wood flour, 1 to 4 percent of wheat bran, 0.5 to 2 percent of white sugar and 0.5 to 2 percent of gypsum powder; preparing the mixture into a culture material, bottling or bagging the culture material, sterilizing the culture material at the temperature of between 100 and 125 DEG C, inoculating the culture material according to 8 to 15 percent of the inoculum concentration, and performing management according to a conventional management method for fungus-germination and the fruiting of the agrocybe aegerita. The method uses the multi-grain concentrated fragrance type liquor waste lees in stead of cotton seed hulls to produce the agrocybe aegerita and reduces the production cost, and simultaneously, because the fresh multi-grain concentrated fragrance type liquor waste lees are used, the method reduces the link of tedding the waste lees, and protects the environment.

Description

A kind of method for producing agrocybe aegerita
Technical field
The present invention relates to a kind of Edible Fungi method, mainly is to utilize many grain giving off a strong fragrance spirit distiller grain to produce the production method of tea tree mushroom.
Background technology
Tea tree mushroom has another name called tea firewood mushroom, tea mushroom, oil tea mushroom, belongs to Eumycota, Basidiomycetes, and Agaricales, excrement is embroidered umbrella section, is a kind of famous edible and medicinal fungi.Since tea tree mushroom in 1972 is by domesticating and cultivating, selected suitable tame high yield and high quality bacterial strain through production practices for many years, formed comparatively ripe culture technique.Main cultivation prescription has at present: (1) cotton seed hulls 75%, wheat bran 20%, sucrose 2%, superphosphate 1%, calcium carbonate 1%, land plaster 1%; (2) cotton seed hulls 83%, wheat bran 10%, corn flour 5%, land plaster 1%, calcium carbonate 1%.In these 2 prescriptions, cotton seed hulls mainly provides slow effect carbon source and partial nitrogen source, and water conservation is ventilative; Wheat bran mainly provides nitrogenous source, part carbon source and growth factor, the quick-acting carbon sources that sucrose and corn flour more easily absorb when the mycelium germination field planting is provided; When providing calcium, magnesium ion, land plaster and calcium carbonate also has the effect of ph stability agent to a certain degree.Utilize above-mentioned 2 kinds of prescriptions to produce tea tree mushroom technology maturation at present, all there is plantation the nationwide.From be inoculated into pluck the first damp mushroom time generally about 80 days, conversion ratio is all at 90%-110%.But above-mentioned 2 kinds of prescriptions all need to use a large amount of cotton seed hullss and wheat bran, cause production cost higher, but not the cotton growing area cost are higher.Add, nearly all edible fungus culturing all is to be main raw material(s) (accounting for more than 60% of composts or fertilisers of cultivating composition) with the cotton seed hulls at present, along with further developing of China's mushroom industry, the imbalance between supply and demand of cotton seed hulls also can further strengthen (play), and cost also can constantly increase.
Summary of the invention
The purpose of this invention is to provide the production method that substitutes cotton seed hulls production tea tree mushroom with many grain giving off a strong fragrance spirit distiller grain, to reduce production costs.
The object of the present invention is achieved like this: many grain aroma type spirit distiller grain is to add a large amount of chaff shells after the plurality of cereals mixed culture fermentation, and the product behind the liquor is produced in distillation.Because mainly utilize part carbon source and a small amount of nitrogenous source in the grain in the liquor production process, so contain in many grain aroma type spirit distiller grain abundant quick-acting, imitate carbon source, nitrogenous source and growth factor late, chaff shell in many grain aroma type spirit distiller grain also has loose composts or fertilisers of cultivating simultaneously, make the medium consolidation, have air permeability and good.
The lime that fresh aroma type spirit distiller grain is added 3%-5% is mixed thoroughly, regulates the pH value to 9-12; And by losing poor 80%-95%, wood chip 1%-10%, wheat bran 1%-4%, 0.5%-2%, land plaster 0.5%-2%, mix, make composts or fertilisers of cultivating, bottling or pack, sterilization, bacterium, management of producing mushroom management by methods, fruiting are sent out according to traditional tea tree mushroom in inoculum concentration inoculation back by 8%-15% (producing the proportion of bacterial classification and composts or fertilisers of cultivating).
As preferably, because the material of meeting inhibition mycelial growths such as aldehyde, alcohol is more in the fresh spirit distiller grain, can (press cotton seed hulls 75%-83% at composts or fertilisers of cultivating surface coverage one deck tradition cotton seed hulls composts or fertilisers of cultivating, wheat bran 6%-10%, corn flour 2%-5%, land plaster 0.5%-2%, calcium carbonate 0.5%-2% preparation), make bacterial classification a procedure of adaptation be arranged, also make the composts or fertilisers of cultivating surface mycelia formation fruit body of easily twisting together simultaneously for losing poor composts or fertilisers of cultivating.
Because when using bag to plant, be that the composts or fertilisers of cultivating of main component is not easy to compress to lose poor, to lose poor water retention property poor slightly in addition, so add thin wood chip to reduce the space between composts or fertilisers of cultivating when using bag to plant, the enhancing water retention capacity also can improve the part carbon source, stablizes the C/N ratio.When using bottle to plant, can be less with or without wood chip.
The present invention adopts traditional tea tree mushroom cultivation management method, and fruiting time, conversion ratio and the quality that is obtained is all suitable with traditional composts or fertilisers of cultivating cultivation effect.
Effect of the present invention is: the raw materials for production cost declines to a great extent.According to the price of above-mentioned various main raw material(s)s of in November, 2008, the cost of material of producing tea firewood mushroom with traditional composts or fertilisers of cultivating prescription and prescription of the present invention in Sichuan compares, and uses production technology of the present invention, and the raw materials for production cost descends 80%.Simultaneously, ted link, protected environment owing to using fresh many grain aroma type spirit distiller grain, reduced losing to be pickled with grains or in wine.In addition, the present invention has found a kind of new technology of utilizing aroma type spirit distiller grain, and also the problem that too relies on cotton seed hulls for China's Edible Fungi has found a kind of new method and thinking.
Embodiment
Below in conjunction with example, the present invention is described further:
Embodiment
Embodiment 1:
Get fresh many grain aroma type spirit distiller grain (distillery from the wine rice steamer, come out 5 days with interior all can), measure pH value, after roughly calculating, add pulverized limestone and mix thoroughly, adjusting pH value is to 10-11.And by losing poor 90%-94%, wheat bran 2%-4%, white sugar 0.5%-1%, land plaster 0.5%-1% mixes.When mixing thoroughly, slowly add water, to hold composts or fertilisers of cultivating moisture, water droplet is arranged between webs but not following for standard.Accomplish to mix when noting spice, absorb water evenly, acid-base value is even.With the composts or fertilisers of cultivating of the mixing volume of packing into is in 800 milliliters the mushroom bottle, is filled to shoulder, with hand compacting slightly, cleans out bottleneck and seals with double-deck brown paper or 4 layers of newspaper and thin cotton thread then.Sterilized 3-4 hour for 100 ℃-125 ℃.After the bacterium bottle is cooled to room temperature, original seed directly is inoculated in the composts or fertilisers of cultivating surface, press the inoculum concentration inoculation of 10%-15% (producing the proportion of bacterial classification and composts or fertilisers of cultivating), require to cover the composts or fertilisers of cultivating surface comprehensively, and compress 1 centimetre-2 centimetres of original seed height (thick) slightly, bottle interior medium and outside air are separated, to reduce infection rate and to promote mycelial growth.After inoculation finishes, place and send out bacterium 40-50 days in 23 ℃-28 ℃ the culturing room and get final product purseful, require to cultivate room ventilation, breathe freely, lucifuge, relative air humidity is lower than 70%.After mycelia sends out fully and expires, with the scattered light irradiation, to stimulate the mycelia knot.The bacterium bag of respectively filling a prescription that covers with mycelia is moved into the fruiting room, mushroom room temperature is controlled at 18 ℃-26 ℃, with spray pattern control relative air humidity is 85%-95%, suitably ventilates, through 12-20 days latters stage of ripening, opening bag waters and formed the mushroom flower bud in 6-10 days, can reach eight fens maturations through 7-15 days again, the cap color is thin out gradually, diameter 2-3 centimetre, when mycoderm is about to break, has met the commodity mushroom and required and to pluck.Fruiting phase 2-3 month, can adopt 4-5 tide mushroom continuously, conversion ratio can reach 85%-95%.Under identical management, harvesting condition, it is consistent that one-tenth mushroom quality and traditional cultivation technology are produced into mushroom.
Embodiment 2:
Get fresh many grain aroma type spirit distiller grain (distillery from the wine rice steamer, come out 5 days with interior all can) measure pH value, after roughly calculating, add pulverized limestone and mix thoroughly, adjusting pH value is to 10-11.According to losing poor 80%-85%, wheat bran 2%-4%, wood chip 6%-10%, land plaster 0.5%-2% prescription is mixed thoroughly after adding various auxiliary materials successively.When mixing thoroughly, slowly add water, to hold composts or fertilisers of cultivating, water droplet is arranged between webs but not following for standard.Accomplish to mix when noting spice, absorb water evenly, acid-base value is even.With the composts or fertilisers of cultivating that the ferments specification of packing into is in the polypropylene plastic sack of 17cm * 33cm * 0.06cm, every sacked material 600g, conventional cotton seed hulls composts or fertilisers of cultivating (cotton seed hulls 83%, wheat bran 10%, the corn flour 5% mixed that add 1 centimetre-2 cm thick again on the composts or fertilisers of cultivating surface, land plaster 1%, calcium carbonate 1%), and real with hand, the leveling charge level, clean out sack, put the plastics collar.Sterilized 3-4 hour for 100 ℃-125 ℃.Press the inoculum concentration inoculation of 8%-15% (producing the proportion of bacterial classification and composts or fertilisers of cultivating), a bacterium, fruiting, harvesting condition are identical with embodiment 1.The tea tree mushroom bag was planted about 3-4 of fruiting phase month, can adopt 4-5 tide mushroom continuously, and conversion ratio can reach 90%-100%, under identical management, harvesting condition, and commodity mushroom output and traditional cultivation technology basically identical.

Claims (2)

1. method for producing agrocybe aegerita, it is characterized in that: the lime that fresh aroma type spirit distiller grain is added 3%-5% is mixed thoroughly, regulates the pH value to 9-12; And by losing poor 80%-95%, wood chip 1%-10%, wheat bran 1%-4%, white sugar 0.5%-2%, land plaster 0.5%-2%, mix, make composts or fertilisers of cultivating, bottling or pack, 100 ℃ of-125 ℃ of sterilizations, bacterium, management of producing mushroom management by methods, fruiting are sent out according to traditional tea tree mushroom in inoculum concentration inoculation back by 8%-15%.
2. a kind of method for producing agrocybe aegerita as claimed in claim 1 is characterized in that: composts or fertilisers of cultivating surface coverage one deck tradition cotton seed hulls composts or fertilisers of cultivating, and its prescription is cotton seed hulls 75%-83%, wheat bran 6%-10%, corn flour 2%-5%, land plaster 0.5%-2%, calcium carbonate 0.5%-2% preparation.
CN200810233380A 2008-12-17 2008-12-17 Method for producing agrocybe aegerita Pending CN101743851A (en)

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Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101941867A (en) * 2010-08-05 2011-01-12 镇江市食用菌研究所 Drumstick mushroom culture medium and preparation processing method thereof
CN101941853A (en) * 2010-08-05 2011-01-12 镇江市食用菌研究所 Low-carbon compound edible fungus culture material
CN101946638A (en) * 2010-09-20 2011-01-19 福建省曲斗香酒业有限公司 Method for culturing cordyceps militaris by distilled grain
CN102007847A (en) * 2010-10-26 2011-04-13 镇江市京口区瑞京农业科技示范园 Method for cultivating agrocybe aegerita by using light industrial waste residues
CN102047813A (en) * 2010-10-20 2011-05-11 天津渔阳酒业有限责任公司 Sterilization and inoculation process for edible fungus culture material
CN102126886A (en) * 2010-11-25 2011-07-20 张若泰 Straw ethanol waste substrate for culturing edible fungi
CN102584363A (en) * 2011-12-01 2012-07-18 浙江塔牌绍兴酒有限公司 Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium
CN102577839A (en) * 2011-12-01 2012-07-18 浙江塔牌绍兴酒有限公司 Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium
CN102674938A (en) * 2012-06-11 2012-09-19 宁德师范学院 Formula technology for agrocybe chaxingu strain culture medium and process for culturing strain
CN103145501A (en) * 2013-03-20 2013-06-12 贵州省土壤肥料研究所 Culture base stock used for cultivating edible fungi and preparation method thereof
CN103435402A (en) * 2013-08-03 2013-12-11 邬金飞 Agrocybe cylindracea cultivation material composition and preparation method thereof
CN103975763A (en) * 2014-05-14 2014-08-13 河北沃达生物技术有限公司 Agrocybe cylindracea cultivation method
CN104478605A (en) * 2014-12-19 2015-04-01 苏州市经纬农产品有限公司 Agrocybe aegerita culture medium and preparation method thereof
CN106518464A (en) * 2016-12-07 2017-03-22 重庆维得鲜农业发展有限公司 Culture medium of flammulina velutipes and preparation method of culture medium
CN107466682A (en) * 2017-10-18 2017-12-15 灵川县海洋乡欣源食品有限公司 A kind of agrocybe implantation methods
CN107950298A (en) * 2017-12-12 2018-04-24 四川才阁机械有限公司 A kind of agrocybe cultivation formula and preparation method thereof

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101941867A (en) * 2010-08-05 2011-01-12 镇江市食用菌研究所 Drumstick mushroom culture medium and preparation processing method thereof
CN101941853A (en) * 2010-08-05 2011-01-12 镇江市食用菌研究所 Low-carbon compound edible fungus culture material
CN101941867B (en) * 2010-08-05 2012-05-30 镇江市食用菌研究所 Coprinus comatus culture medium and preparation treatment method thereof
CN101941853B (en) * 2010-08-05 2012-05-30 镇江市食用菌研究所 Low-carbon composite edible fungus culture material
CN101946638A (en) * 2010-09-20 2011-01-19 福建省曲斗香酒业有限公司 Method for culturing cordyceps militaris by distilled grain
CN101946638B (en) * 2010-09-20 2011-12-28 福建省曲斗香酒业有限公司 Method for culturing cordyceps militaris by distilled grain
CN102047813A (en) * 2010-10-20 2011-05-11 天津渔阳酒业有限责任公司 Sterilization and inoculation process for edible fungus culture material
CN102007847A (en) * 2010-10-26 2011-04-13 镇江市京口区瑞京农业科技示范园 Method for cultivating agrocybe aegerita by using light industrial waste residues
CN102126886A (en) * 2010-11-25 2011-07-20 张若泰 Straw ethanol waste substrate for culturing edible fungi
CN102577839A (en) * 2011-12-01 2012-07-18 浙江塔牌绍兴酒有限公司 Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium
CN102577839B (en) * 2011-12-01 2013-07-31 浙江塔牌绍兴酒有限公司 Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium
CN102584363B (en) * 2011-12-01 2013-11-27 浙江塔牌绍兴酒有限公司 Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium
CN102584363A (en) * 2011-12-01 2012-07-18 浙江塔牌绍兴酒有限公司 Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium
CN102674938A (en) * 2012-06-11 2012-09-19 宁德师范学院 Formula technology for agrocybe chaxingu strain culture medium and process for culturing strain
CN103145501B (en) * 2013-03-20 2015-03-04 贵州省土壤肥料研究所 Culture base stock used for cultivating edible fungi and preparation method thereof
CN103145501A (en) * 2013-03-20 2013-06-12 贵州省土壤肥料研究所 Culture base stock used for cultivating edible fungi and preparation method thereof
CN103435402B (en) * 2013-08-03 2015-04-22 邬金飞 Agrocybe cylindracea cultivation material composition and preparation method thereof
CN103435402A (en) * 2013-08-03 2013-12-11 邬金飞 Agrocybe cylindracea cultivation material composition and preparation method thereof
CN103975763A (en) * 2014-05-14 2014-08-13 河北沃达生物技术有限公司 Agrocybe cylindracea cultivation method
CN104478605A (en) * 2014-12-19 2015-04-01 苏州市经纬农产品有限公司 Agrocybe aegerita culture medium and preparation method thereof
CN106518464A (en) * 2016-12-07 2017-03-22 重庆维得鲜农业发展有限公司 Culture medium of flammulina velutipes and preparation method of culture medium
CN107466682A (en) * 2017-10-18 2017-12-15 灵川县海洋乡欣源食品有限公司 A kind of agrocybe implantation methods
CN107950298A (en) * 2017-12-12 2018-04-24 四川才阁机械有限公司 A kind of agrocybe cultivation formula and preparation method thereof

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Open date: 20100623