CN112129963A

CN112129963A - Automatic analysis device and analysis method

Info

Publication number: CN112129963A
Application number: CN202011029124.4A
Authority: CN
Inventors: 上路央
Original assignee: Hitachi High Technologies Corp
Current assignee: Hitachi High Tech Corp
Priority date: 2015-09-17
Filing date: 2016-07-27
Publication date: 2020-12-25
Anticipated expiration: 2036-07-27
Also published as: JPWO2017047240A1; CN108027380A; CN112129963B; JP6698665B2; WO2017047240A1; CN108027380B

Abstract

The invention provides an automatic analysis device and an analysis method. The disposable reaction vessels are collected into a disposal part after analysis, but if the reaction vessels are collectively stored in a part of the disposal part, there is a problem that the contents overflow. Further, when the height of the reaction vessel is equalized by vibrating the waste portion itself, there is a problem that the interference caused by the vibration affects the measurement result, and the reliability of the analysis is lowered. The reaction vessel disposal unit includes a reaction vessel disposal unit configured to dispose of a reaction vessel after completion of measurement, a reaction vessel transfer mechanism configured to transfer the reaction vessel from the reaction vessel holding unit to the reaction vessel disposal unit and dispose the reaction vessel to the reaction vessel disposal unit, and a control unit configured to control an operation of the reaction vessel transfer mechanism so that disposal of the reaction vessel in the reaction vessel disposal unit is prevented from being continuously performed at a predetermined position. Thus, an automatic analyzer capable of maintaining high reliability of analysis and equalizing the storage of used reaction vessels is provided.

Description

Automatic analysis device and analysis method

The present invention is a divisional application of an invention having an application number of 201680050816.2 (International application number of PCT/JP2016/071948), an invention name of "automatic analyzer", and an application date of 2016, 7, and 27.

Technical Field

The present invention relates to an automatic analyzer for automatically analyzing components contained in a biological sample such as blood, and more particularly to an automatic analyzer including a disposal unit for storing a used reaction container.

Background

As an apparatus for analyzing a target component contained in a biological sample such as blood, the following automatic analyzer is widely used: the reaction solution to be analyzed, in which a sample and a reagent are mixed, is irradiated with light from a light source, and the amounts of transmitted light and scattered light having single or multiple wavelengths obtained by the irradiation are measured.

Automatic analyzers are provided with devices for biochemical analysis for quantitatively and qualitatively analyzing target components in biological samples, such as in the fields of biochemical tests and hematological tests, and devices for blood coagulation analysis for measuring the coagulation ability of blood as a sample.

In particular, in the latter analysis, since the reaction solution changes from a liquid state to a solid state by the coagulation reaction of blood, the reaction container containing the reaction solution used for the analysis needs to be discarded and collected. There are the following problems here: when used reaction vessels or the like are collected in a disposal part by natural falling, the reaction vessels are gathered at a specific part of the disposal part and overflow, and when the overflowing reaction vessels are picked up by a human hand, secondary infection or the like is caused.

Patent document 1 describes an apparatus having a mechanism for collecting used sheets and dishes in a disposable storage container by natural dropping, vibrating the storage container itself back and forth, left and right, or up and down by reciprocating linear motion, and leveling the sheets and dishes stored therein by the vibration, as a technique for preventing such reaction containers and the like from being gathered at a certain specific location.

Documents of the prior art

Patent document

Patent document 1: japanese laid-open patent publication No. 9-127127

Disclosure of Invention

Problems to be solved by the invention

However, in the method described in patent document 1, although the heights of the sheets and the dishes inside can be equalized by the vibration of the container itself, a dedicated mechanism for vibrating the container needs to be separately provided, which makes the apparatus complicated and large-sized. Further, there is a problem that the vibration of the container itself is transmitted to the detection unit of the optical system, and affects the measurement result.

The purpose of the present invention is to realize a highly reliable analysis in which the influence of disturbance such as vibration on the measurement result is suppressed while preventing the accumulation of used reaction vessels while saving space.

Means for solving the problems

As one aspect for solving the above problems, there is provided an automatic analyzer and an analysis method using the same, the automatic analyzer including: a sample dispensing mechanism that dispenses a sample into a reaction vessel; a reagent dispensing mechanism that dispenses a reagent into a reaction vessel; a measuring unit including a reaction container holding unit for holding the reaction container, a light source for irradiating a mixed solution of a sample and a reagent contained in the held reaction container with light, and a detecting unit for detecting the irradiated light; a reaction vessel discarding unit configured to discard the reaction vessel after the measurement is completed; a reaction container transfer mechanism for transferring the reaction container from the reaction container holding portion to the reaction container discarding portion and discarding the reaction container to the reaction container discarding portion; and a control unit that controls an operation of the reaction container transfer mechanism, wherein the control unit controls the operation of the reaction container transfer mechanism so that the reaction container is prevented from being continuously discarded at a predetermined position in the reaction container discarding unit.

Effects of the invention

According to the above aspect, the reaction container transfer mechanism operates so that the point and timing at which the reaction containers are discarded to the discard section do not overlap, and therefore, it is possible to realize a highly reliable analysis in which the influence of the interference due to vibration or the like on the measurement result is suppressed while preventing the used reaction containers in the discard section from being accumulated at a specific location while saving space.

Drawings

Fig. 1 is a diagram showing a basic configuration of an automatic analyzer according to the present embodiment.

Fig. 2 is a diagram showing a configuration of a reaction vessel disposal unit without a lid portion according to the present embodiment (first embodiment).

Fig. 3 is a diagram showing a configuration of a reaction vessel disposal unit having a lid portion with a plurality of opening portions according to the present embodiment (second embodiment).

Fig. 4 is a diagram showing a configuration of a reaction vessel disposal unit having a lid portion with a groove-like opening portion according to the present embodiment (third embodiment).

Fig. 5 is a diagram showing a configuration of a reaction vessel disposal unit according to the present embodiment (fourth embodiment) having a lid portion having a plurality of opening portions and having a plurality of divided housing spaces therein.

Fig. 6 is a diagram showing a configuration of a reaction vessel disposal unit having a detector for detecting a storage state of a reaction vessel therein according to the present embodiment (fifth embodiment).

Fig. 7 is a flowchart illustrating the operation of the reaction container transfer mechanism according to the storage state of the reaction containers therein in the present embodiment (fifth embodiment).

Fig. 8 is a diagram showing a basic configuration of an automatic analyzer including a two-module blood coagulation analyzer according to the present embodiment (sixth embodiment).

Fig. 9 is a diagram showing a configuration of a blood coagulation analyzer of an automatic analyzer according to the present embodiment (sixth embodiment).

Detailed Description

Hereinafter, embodiments for carrying out the present invention will be described in detail with reference to the drawings. In the drawings, the description of the components having the same function is omitted.

< basic Structure of apparatus >

Fig. 1 shows a basic configuration of an automatic analyzer according to the present embodiment. Here, an example of an apparatus for performing blood coagulation analysis will be described as one mode of an automatic analyzer. As shown in the figure, the automatic analyzer 100 mainly includes a sample disk 102, a reagent disk 104, a sample dispensing mechanism 105, a sample dispensing mechanism cleaning unit 106, a reaction container installation unit 108, a reaction container transfer mechanism 109, a reaction container supply unit 110, a detection unit 111, a reagent dispensing mechanism 112, a reagent dispensing mechanism cleaning unit 113, a reaction container disposal unit 114, an operation unit 115, a control unit 116, a storage unit 117, an interface 118, and the like.

The sample disk 102 is a disk-shaped unit that is rotatable clockwise and counterclockwise, and a plurality of sample containers 101 for holding samples or quality control samples can be arranged on the circumference thereof.

The reagent disk 104 is a disk-shaped unit that is rotatable clockwise and counterclockwise in the same manner as the sample disk 102, and a plurality of reagent containers 103 containing reagents containing components that react with components of the respective test items contained in the samples can be arranged on the circumference thereof. Although not shown in the figure, the reagent disk 104 may be configured to be capable of cooling the reagent in the disposed reagent container 103 by providing a cooling mechanism or the like.

The sample dispensing mechanism 105 sucks the sample held in the sample container 101 of the sample disk 102 and discharges the sample into the reaction container 107 provided in the sample dispensing port 119. Here, the operation of the sample dispensing mechanism 105 is controlled based on an instruction from the control unit 116 in accordance with the operation of a sample syringe pump, not shown.

The sample dispensing mechanism cleaning unit 106 is a mechanism for cleaning the sample dispensing mechanism 105 with cleaning water. The timing of the water washing is controlled based on an instruction from the control unit 116.

The reagent dispensing mechanism 112 suctions the reagent held in the reagent container 103 of the reagent disk 104, and dispenses the reagent into the reaction container 107 into which the sample is dispensed, the reaction container being disposed in the reaction container disposition unit 108 of the detection unit 111. Here, the operation of the reagent dispensing mechanism 112 is controlled based on an instruction from the control unit 116 in accordance with the operation of a not-shown reagent syringe pump.

The reagent dispensing mechanism cleaning unit 113 is a mechanism for cleaning the reagent dispensing mechanism 112 with cleaning water. The timing of the water washing is controlled based on an instruction from the control unit 116.

Note that, although the description is given here of a configuration in which the sample dispensing mechanism cleaning unit 106 and the reagent dispensing mechanism cleaning unit 113 are provided separately, they may be a single unit.

The reaction container supply unit 110 is configured to be able to hold a plurality of reaction containers 107 before analysis.

The reaction vessel transfer mechanism 109 transfers the reaction vessel 107 for analysis from the reaction vessel supply unit 110 and carries it into the sample dispensing port 119. The reaction vessel 107 into which the sample has been dispensed is carried out of the sample dispensing port 119, transferred, and carried into the reaction vessel installation section 108 of the detection unit 111. After the analysis is completed, the reaction vessel 107 is carried out of the reaction vessel installation part 108, and transferred and discarded to the reaction vessel discarding part 114.

The detection unit 111 includes one or more (six in this embodiment) reaction container installation parts 108 for installing the reaction containers 107, and an optical system including a light source and a detection part (photosensor), which are not shown, and is constituted by a photodiode. The light source irradiates light to a reaction vessel 107, which is inserted into a reaction vessel installation part 108 and contains a reaction solution that is a mixed solution of a sample and a reagent to be analyzed, and measures the light intensity of scattered light, transmitted light, and the like obtained from a substance generated by a reaction occurring in the reaction solution. For example, in the case of detecting scattered light, each reaction vessel installation section 108 has a light source disposed below and a detection section disposed on a side surface. At this time, the detection unit performs light-current conversion on the received scattered light, and outputs a photometric signal indicating the intensity of the received light to an a/D converter, not shown. The measurement signal of the light intensity a/D-converted by the a/D converter is transmitted to the control section 116 and the storage section 117 via the interface 118.

For example, in a blood coagulation test item, when a sample and a reagent react, fibrin is precipitated as time passes. Then, the amount of scattered light increases with the deposition of fibrin. By detecting the amount of light, the fibrinogen amount (Fbg) in the sample can be determined. Further, by using reagents corresponding to the respective test items and monitoring the light amount in the same manner, other blood coagulation test items such as Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) can be analyzed.

The reaction vessel disposal unit 114 is a means for disposing of the reaction vessel 107 used for the analysis. More specifically, the reaction vessel 114 includes an opening serving as a disposal port and a storage box disposed below the opening and not shown in the figure.

The operation unit 115 includes input terminals such as a keyboard, a mouse, and an operation screen displayed on the display unit as input means, and inputs analysis items of a sample to be analyzed from the keyboard and the operation screen to the control unit 116.

The control unit 116 controls the operations of the sample disk 102, the reagent disk 104, the sample dispensing mechanism 105, the sample dispensing mechanism cleaning unit 106, the reaction container transfer mechanism 109, the detection unit 111, the reagent dispensing mechanism 112, and the reagent dispensing mechanism cleaning unit 113 based on the input from the operation unit 115, and performs all the controls of the operations of the various structures constituting the automatic analyzer 100, condition settings, and the like. The control unit processes the measurement signal input from the detection unit 111 via the interface 118, and performs data processing for obtaining the concentration of the target component based on the detection result, calculating the blood coagulation time, and identifying the abnormal portion. Details will be described later.

The storage unit 117 stores input information from the operation unit 115, operation information of the sample tray 102 and the like, and reagent and sample information and the like.

The interface 118 mediates operation information of the sample disk 102 and the like, input information from the operation unit 115, operation information from the control unit 116, and information stored in the storage unit 117.

The automatic analyzer 100 according to the embodiment of the present embodiment configured as described above analyzes the sample in the order of sample dispensing, reagent dispensing, photometry, disposal of the reaction vessel 107, and data processing. The basic analysis flow will be described in detail below.

A plurality of reagent vessels 103 are arranged in a row on the circumference of the reagent disk 104, and when selection of an analysis item and an analysis start instruction are received from the operation unit 115, the reagent disk is rotated clockwise or counterclockwise according to the analysis item until a target reagent vessel 103 is transferred to the position below the reagent dispensing mechanism 112. A predetermined amount of the reagent in the reagent container 103 is dispensed by a reagent syringe (not shown) connected to the reagent dispensing mechanism 112. A plurality of reaction vessels 107 are arranged in the vertical and horizontal directions in the reaction vessel supply section 110. The reaction vessel transfer mechanism 109 transfers and carries the reaction vessels 107 from the reaction vessel supply unit 110 to the reaction vessel installation unit 108 via the reagent dispensing ports 119 in a predetermined order.

The sample disk 102 has a plurality of sample containers 101 arranged in a circumferential array, and is rotated clockwise or counterclockwise according to the order of samples to be analyzed, and conveyed to a position below the sample dispensing mechanism 105.

A predetermined amount of the sample in the sample container 101 is dispensed to the reaction container 107 provided in the sample dispensing port 119 by a sample syringe (not shown) connected to the sample dispensing mechanism 105. Then, the reagent dispensing mechanism 112 suctions the reagent from the reagent vessel 103 and dispenses the reagent into the reaction vessel 107 containing the sample, which is provided in the reaction vessel installation part 108 of the detection unit 111.

The light is irradiated from the light source, and the progress of the reaction in the reaction solution, which is the mixed solution of the sample and the reagent stored in the reaction container 107, is collected by the detection unit composed of the photodiode described above and detected by the detection unit 111. The detected data, the number of the use detection unit, the type of the use sample, the set position on the sample disk 102, the type of the use reagent, and the set position on the reagent disk 104 are stored in the storage section 117.

When the reaction and measurement of the reaction solution held in the reaction container 107 are completed, the reaction container 107 is discarded to the reaction container discarding part 114 by the reaction container transfer mechanism 109 while holding the mixed solution after the reaction.

First embodiment

FIG. 2 is a diagram showing the structure of a reaction vessel disposal unit without a lid unit according to this embodiment. The reaction vessel disposal section 114 shown in fig. 1 is constituted by an open storage box 201 in the present embodiment. The reaction container transfer mechanism 202 transfers and discards the used reaction container 203 after the analysis to the storage box 201 via the transfer path 204.

At this time, when the position where the reaction container transfer mechanism 202 discards the reaction container 203 is always the same, the reaction container 203 is stored at a fixed position in the storage box 201. Therefore, the reaction vessel transfer mechanism 202 of the present embodiment can arbitrarily determine the disposal point on the storage box 201 so that the disposal operation of the reaction vessel 203 is not continuously performed at a predetermined position. The place of disposal is not limited as long as the storage box 201 is provided, and, for example, the disposal points can be shifted in a discontinuous manner by slightly changing the driving amount of a motor (not shown) that controls the position of the reaction vessel transfer mechanism 202. Therefore, if the discarded point can be shifted by controlling the operation of the reaction vessel transfer mechanism 202, the reaction vessel 203 can be prevented from being stored at a specific position in the storage box 201 while suppressing the influence of disturbance due to vibration. This makes it possible to uniformly store the reaction vessels 203 in the storage box 201 in the automatic analyzer which maintains the reliability of the measurement results and saves space.

Second embodiment

In the above-described embodiment, the structure of the reaction vessel disposal unit having the storage box which does not have the lid and has an open upper surface is described. In the present embodiment, the structure of a reaction vessel disposal unit constituted by a storage box having a lid portion with a plurality of opening portions will be described with reference to fig. 3.

In this configuration, the reaction container transfer mechanism 302 transports the used reaction containers 303 after the analysis is completed to the plurality of openings 304 formed in the lid portion 305 of the storage box 301 via the transport path 306 and discards the reaction containers. In the present figure, the case where the number of the opening portions 304 formed in the cover portion 305 is five is shown, but the number is not limited and may be changed as needed.

Here, the reaction container transfer mechanism 302 holds the used reaction container 303 set in the reaction container setting unit 108 shown in fig. 1, and then moves the reaction container 303 to a position directly above any one of the plurality of openings 304 through the conveyance path 306 to discard the reaction container 303. The order of discarding can be set in a fixed sequence as shown in fig. 1 → 2 → 3 → 4 → 5 → 1 → …, or can be changed as needed. Therefore, by changing the order of discarding the openings formed in plurality in a fixed order, the reaction vessels 303 can be prevented from being stored at fixed positions in the storage box 301, and the heights of the stored reaction vessels 303 can be equalized.

Further, by providing the lid 305, it is possible to prevent falling objects and the like other than the reaction containers 303 from falling into the storage box 301. Here, the reaction container 303 holds a reaction solution, which is a mixed solution of a sample and a reagent, and a precipitate generated after the reaction, and contains a substance having a risk of a dangerous infectious disease depending on the sample. Therefore, if an object other than the reaction container 303 is collected in the storage box 301, there is a risk of secondary infection due to the user touching the inside of the storage box 303. According to the present embodiment, since the cover 305 is provided, it is possible to prevent the objects other than the reaction containers 303 from being collected into the housing box 301, and thus such a risk can be avoided.

Third embodiment

Next, the structure of the reaction vessel disposal unit having the lid portion with the groove-like opening portion according to the present embodiment will be described with reference to fig. 4. In the second embodiment described above, the configuration in which the lid portion 305 is formed with the plurality of openings 304 has been described, and here, the lid portion 405 includes the single opening 404 formed in a groove shape and including the plurality of disposal points.

In this configuration, the reaction container transfer mechanism 402 transfers the used reaction containers 403 after the analysis is completed to the single opening 404 formed in a groove shape in the lid portion 405 of the storage box 401 via the transfer path 406, and a plurality of waste dots included in the opening 404 are discarded, and the order of discarding may be set in a fixed sequence as shown in, for example, P1 → P2 → P3 → P4 → P5 → P6 → … of the opening 404 in the drawing, or may be changed as necessary.

The shape of the opening 405 is not a circular shape corresponding to the reaction vessel 403 as in the second embodiment but a groove shape, so that a plurality of disposal points can be included in one opening 405. The reaction container transfer unit 402 can discard the reaction containers 403 at any point as long as it is on the opening 404, and after discarding the reaction containers 403 at a certain point, the distance between the discarded points can be increased by increasing the movement distance, and conversely, the movement distance can be shortened so that the distance between the discarded points is several millimeters.

According to the present embodiment, the reaction vessel 403 can be discarded into the storage box 401 over a wide range in one opening 404. By discarding the reaction vessels 403 in the storage box 401 at a plurality of disposal points of the single opening 404, the heights of the reaction vessels 403 in the storage box 401 can be equalized. In addition, since the disposal reaction vessels 403 can be disposed in the single opening 404 with a shift of several millimeters as described above, the degree of freedom of disposal points increases. This makes it possible to more accurately equalize the heights of the reaction vessels 403 in the storage box 401.

The shape, arrangement, and disposal procedure of the opening 404 can be changed as appropriate, in addition to the above-described configuration.

Fourth embodiment

In this embodiment, a configuration of a reaction vessel disposal unit having a lid portion with a plurality of opening portions and having a plurality of storage spaces divided therein and a mode of changing the storage space in the storage box of the disposal reaction vessel in accordance with the state of the sample using this configuration will be described with reference to fig. 5.

In this configuration, the reaction container transfer mechanism 502 transfers the used reaction containers 503 after the analysis is completed to the plurality of openings 604 formed in the lid 505 of the storage box 501 via the transfer path 506, and discards any one of the plurality of storage spaces partitioned by the partition member 505 in the storage box 501. Here, the inside of the housing box 501 is divided into two spaces, a first space 501a and a second space 501b, by a partition member 505.

In blood coagulation analysis, there is a difference in measurement items, and a blood sample as a sample and a reagent react with each other in several seconds for a rapid item, and the mixed solution starts coagulation. However, in the case of some samples lacking a blood coagulation factor, the sample does not coagulate for several seconds after mixing with the reagent, and sometimes does not coagulate even after several minutes.

In this case, the automatic analyzer sets a maximum time for which measurement is continued, such as a maximum measurement time. When the time has elapsed, the measurement is terminated without obtaining the blood coagulation time as the analysis result. At this time, the sample is kept in a substantially liquid state in the reaction container 503. When the reaction vessel 503 holding the liquid specimen is discarded into the common space in the housing box 501 by the reaction vessel transfer mechanism 502 similarly to the other reaction vessel 503, that is, the reaction vessel 503 containing the mixture which has been changed into a solid after the completion of the blood coagulation reaction, there is a problem that the liquid in the reaction vessel 503 is scattered and the inside of the housing box 501 is contaminated.

In the present embodiment, for example, the reaction container 503, which is directly accommodated in a state where the mixed solution of the sample and the reagent does not solidify even when the maximum measurement time is reached, is discarded from the position No. five or No. six of the opening 504 and is collected into the first space 501a in the housing box 501. On the other hand, when the mixed solution of the sample and the reagent is solidified and changed to a solid state as in the usual case, the reaction container 503 is discarded from any position of the first to fourth openings 504 and collected into the second space 501b in the housing box 501. More specifically, when the amount of optical change (time-out) necessary to obtain the blood coagulation time is not obtained by the detection unit of the detection means 111 even when the maximum measurement time has elapsed, the control unit 116 determines that the mixed liquid contained in the reaction container 503 for analysis is in a liquid state, and controls the operation of the reaction container transfer mechanism 502 so as to be collected into the first space 501a as described above.

Therefore, by changing the position of the opening 504 in each of the spaces defined by the partition member 505 provided in the housing box 501 in accordance with the state of the blood coagulation reaction, the mixture of the liquid mixed solution and the coagulated solid in the reaction vessel 503 can be separately collected.

Further, by taking measures such as providing a double disposal plastic bag in the first space 501a of the reaction vessel 503 for collecting and storing the liquid-state mixed liquid, it is possible to reduce the risk of contamination in the storage box 501 and contact with the liquid-state mixed liquid or the like scattered from the inside when the reaction vessel 503 is discarded.

The shape, arrangement, and disposal order of the opening 504 can be changed as appropriate, in addition to the above-described configuration.

Fifth embodiment

In this embodiment, a configuration of a reaction container disposal unit having a detector for detecting a storage state of a reaction container therein and an operation of a reaction container transfer mechanism for detecting a storage state of a reaction container in a storage box to more accurately equalize the storage of the reaction container using this configuration will be described with reference to fig. 6 and 7.

In this configuration, the reaction container transfer mechanism 602 transfers the used reaction containers 603 after the end of analysis to the plurality of openings 604 formed in the lid 605 of the storage box 601 via the transfer path 606 and discards the containers.

Here, a plurality of detectors 607 capable of detecting the storage state of the collected reaction containers 603 are provided in the storage box 601. The type of the detector 607 is not particularly limited as long as the presence of the reaction container 603 collected from the corresponding opening 604 can be detected. Here, in order to detect the presence of the reaction container 603 collected from the corresponding opening 604 with high accuracy, the detector 607 is preferably provided below the position of the corresponding opening 604.

When the reaction container transfer mechanism 602 starts the transfer and disposal operations of the reaction containers 603, first, the collection information of the reaction containers 603 stored in the storage unit 117 at the initial stage is read (step 701). Here, the collection information of the reaction vessel 603 is information including the time when the reaction vessel 603 was discarded last time at the present time and the position of the used opening 604.

When the reaction vessel 603 is transferred by the reaction vessel transfer mechanism 602 and continuously discarded into the housing box 601, the reaction vessel 603 is stored under the opening 604 used. Here, when the storage of the reaction vessel 603 is continued up to the setting position of the detector 607, the detector 607 detects the presence of the stored reaction vessel 603 (step 702). In this case, the number of detectable detectors 607 is not limited to one, and a plurality of detectors 607 can detect and store the detected data. If at least one of the plurality of detectors 607 detects the presence of a reaction vessel 603, the process proceeds to step 703.

When the detector 607 detects the storage state of the reaction containers 603 in the storage box 601, the opening 604 in which the storage of the reaction containers 603 is minimum is determined based on the information on the previous disposal time stored in the storage unit 117 and the position of the detector 607 (step 703).

Here, the opening 604 in which the reaction container 603 is stored at the minimum corresponds to the opening 604 in which the presence of the reaction container 603 is not detected in step 702. In this step, when a plurality of openings 604 are not detected as the presence of the reaction vessel 603, the opening 604 at the position where the distance between the previous discarding time obtained in step 701 and the current time is longest is determined as the opening 604 with the small number of the reaction vessels 603 stored.

After the opening 604 determined to have a small amount of the reaction container 603 is determined, the reaction container transfer mechanism 602 transfers the reaction container 603 to the determined position of the opening 604 and discards it in the storage box 601 through the opening 604 (step 704).

Here, when all the detectors 607 in the housing 601 do not detect the presence of the reaction vessel 603, the control unit 116 determines that the storage amount of the reaction vessel 603 is still small. In this case, the opening 604 that is located at the position having the longest distance between the previous used disposal time and the current time among the plurality of openings 604 is determined as the next disposal position. Alternatively, when the opening 604 for disposal cannot be determined in step 703, for example, when all the detectors 607 detect the presence of the reaction container 603, the opening 604 whose disposal time used last time is the longest from the current time among the plurality of openings 604 is determined as the next disposal site (step 705). After the opening 604 of the disposal reaction container 603 is determined, the process proceeds to step 704.

After the reaction vessel 603 is discarded, the position of the used opening 604 and the time for discarding are stored in the storage section 117 (step 706). Here, for example, when the reaction vessel 603 is discarded through the opening 604, the collection information about the opening 604 stored in the previous use is rewritten and stored, and the discarding operation is terminated.

According to this configuration, by providing a plurality of detectors 607 in the storage box 601, the presence of the collected reaction containers 603 can be detected, and the storage status can be checked. Further, by storing information on the position of the opening 604 used at the time of disposal and the time of disposal, it is possible to more accurately determine from which opening 604 among the plurality of openings 604 the reaction vessel 603 is disposed of, and thus it is possible to equalize the height of the reaction vessel 603 collected into the housing box 601.

The present embodiment can also be applied to a configuration having a plurality of disposal points in one opening 604 as in the third embodiment described above.

Sixth embodiment

In the above-described embodiments, a description has been given of an apparatus for performing blood coagulation analysis, which is an example of an automatic analyzer, particularly, an independent type structure which is operated as one independent apparatus.

However, the automatic analyzer for clinical examination has a module type structure in addition to such a stand-alone type, and is connected to analysis units in a plurality of analysis fields such as biochemical analysis, immunoassay, and blood coagulation analysis for the purpose of enhancing the efficiency of the operation of the examination room, and is operated as one apparatus using the entire common sample rack transport line.

In the present embodiment, an example of application of an automatic analyzer including a two-module blood coagulation analyzer will be described with reference to fig. 8 as an example of a module type automatic analyzer.

Fig. 8 is a diagram showing a basic configuration of an automatic analyzer including a two-module blood coagulation analyzer according to the present embodiment. As shown in the figure, the module type automatic analyzer 800 includes a first blood coagulation analyzer 812 and a second blood coagulation analyzer 817 as a plurality of analyzers for analyzing a reaction solution that is a mixed solution of a sample and a reagent, and includes

transfer lines

804 and 805 for transferring a sample rack 801 on which a sample container for storing a sample is mounted in order to supply a sample to each analyzer.

An example of a transport system for transporting a sample rack 801 on which a sample container containing a sample such as plasma to be analyzed is mounted is shown in which: a rack supply unit 802 that supplies the sample rack 801 to the transfer line 804; a rack storage unit 803 for storing the sample rack 801 that has moved on the transport line 805 after the analysis is completed; a transport line (transport direction) 804 for transporting the sample rack 801 to each analysis unit; a conveying line (return direction) 805; a rack standby unit 806 that causes a sample rack 801 to be analyzed to stand by; a rack handling mechanism 807 for transferring the sample rack 801 between the

transfer lines

804 and 805 and the rack standby unit 806 and within the rack standby unit 806; a rack distribution mechanism 809 that distributes the destinations of the racks on the transport line 805 based on the information on the sample rack 801; a rack returning mechanism 808 for moving the distributed sample rack 801 to the rack storage unit 803; an emergency sample rack input unit 810 for inputting a sample rack 801 to be analyzed in an emergency; and a reading unit 811 (transport line) for reading information such as a barcode of the sample rack 801 attached to the transport line 804.

The transport system of the first blood coagulation analyzer 812 disposed along the transport line 804 includes: a reading unit (first blood coagulation analyzer) 816 for checking analysis request information for the samples stored in the sample racks 901 from the transfer line 804; a first rack carrying-in mechanism 814 that receives the sample rack 801 from the carrying line 804; a first dispensing line 813 which includes a sampling region for dispensing a sample and can stand by the sample rack 801 before the dispensing of the sample is started; and a first rack handling mechanism 815 for transferring the sample rack 801 into which the sample has been dispensed to the

transfer lines

804 and 805.

The transport system of the second blood coagulation analyzer 817 arranged along the transport line 804 also includes, in the same manner as the above-described structure of the transport system of the first blood coagulation analyzer 812: a reading unit (second blood coagulation analyzing unit) 821 for checking analysis request information for the sample stored in the sample rack 801 from the transfer line 804; a second rack carrying-in mechanism 819 that receives the sample rack 801 from the carrying line 804; a second dispensing line 818 which includes a sampling region for dispensing a sample and can stand by the sample rack 801 before the dispensing of the sample is started; and a second rack manipulator 820 for transporting the sample rack 801 to the transport line 805 after sample dispensing.

The control unit 822 performs control of operations of various configurations constituting the automatic analyzer 800, such as the above-described transport operation of the sample rack 801, the sample/reagent dispensing operation, the sample rack 801 distribution, carrying in/out operation based on the read information, and the like, and data processing operations such as calculation of blood coagulation time and target component concentration based on the detection result, and condition setting, as a whole. The control unit 822 is connected to an input unit 825 such as a keyboard for inputting various data relating to analysis conditions, instructions from an operator, and the like, a storage unit 823 for storing input information, information read from a sample, a reagent, and the like, information relating to a detection result, and the like, and an output unit 824 for displaying the detection result and various operations of the automatic analysis apparatus 800, and the like. In the present figure, the control unit 822 is connected to each component to control the entire automatic analyzer, but may be configured to include a control unit independent for each component.

Here, the first to fifth embodiments described above can be applied to the structures of the reaction vessel discarding part 828 of the first blood coagulation analyzer 812, the reaction vessel transfer mechanism 830, the reaction vessel discarding part 829 of the second blood coagulation analyzer 817, and the reaction vessel transfer mechanism 831.

In the case of a module-type automatic analyzer, since the processing capacity per unit time is higher than that of a stand-alone type, the amount of reaction containers after disposal is large, and the above-described problem becomes more significant. Therefore, with such a configuration, even in a module-type automatic analyzer, storage of used reaction vessels can be equalized, concentration can be prevented, and high analysis accuracy can be ensured.

Next, the structure of the blood coagulation analyzer described above will be described in more detail with reference to fig. 9. In fig. 9, the present invention includes: a sample dispensing mechanism 917 that dispenses the sample in the sample container stored in the sample rack to the reaction container 901 used for measurement; a sample dispensing port 916 capable of being disposed in the reaction container 901 to be subjected to the sample dispensing operation; a standby unit 911 having a plurality of standby ports 910 for storing reaction containers in a standby state; a reaction container library 902 storing a plurality of reaction containers 901; a reaction container transfer mechanism 912 for transferring the reaction container 901 to and from each position as necessary; a preheating unit 909 that adjusts the temperature to 37 ℃, and includes a plurality of preheating ports 908 that heat up the sample before the blood coagulation time is measured, or the sample after the completion of the pretreatment such as dilution; an analysis unit 907 similarly adjusted to 37 ℃ and provided with a plurality of analysis ports 910 for measuring blood coagulation time; a reagent disk 904 in which a reagent cartridge 903 is arranged in a circumferential shape, the temperature of the reagent cartridge 903 is adjusted to about 10 ℃, and reagent bottles in which reagents are sealed are placed in the reagent cartridge 903; a reagent cassette transfer mechanism 915 for transferring the reagent cassette 903 disposed in the reagent cassette supply part 913 to the reagent tray 904; a reagent information reading unit 905 that reads reagent information from a medium such as a barcode or an RFID to which measurement items, a lifetime, and the like are input, of the reagent cartridge 903 transferred to the reagent disk 904; a reagent cassette storage section 914 that is taken out from the reagent disk 904 by the reagent cassette transfer mechanism 915 and stores the used reagent cassettes 903; a reaction vessel disposal unit 923 for disposing of the used reaction vessel 901; a sample dispensing mechanism cleaning unit 918 for cleaning the sample dispensing mechanism; a first reagent mechanism cleaning unit 920 for cleaning the first reagent dispensing mechanism; and a second reagent dispensing mechanism cleaning unit 922 for cleaning the second reagent dispensing mechanism 921.

The measurement of the blood coagulation time is obtained by calculation in the control unit 922 based on the data of the detected light.

Here, the first to fifth embodiments described above can be applied to the configurations of the reaction vessel discarding section 923 and the reaction vessel transfer mechanism 912 in this figure.

The present invention is not limited to the above-described embodiments, and various modifications are possible. For example, the above-described embodiments are examples explained in detail to explain the present invention easily and understandably, and are not limited to having all the configurations explained. Further, a part of the structure of one embodiment may be replaced with the structure of another embodiment, and the structure of another embodiment may be added to the structure of one embodiment.

Further, the above-described respective structures, functions, processing units, processing means, and the like may be partially or entirely realized by hardware, for example, by designing them on an integrated circuit. The above-described structures, functions, and the like may be realized by software by a processor interpreting and executing a program for realizing the respective functions. Information such as programs, tables, and files for realizing the respective functions can be stored in a memory, a storage device such as a hard disk or an SSD (Solid State Drive), or a storage medium such as an IC card, an SD card, or a DVD.

Note that the information lines and the control lines are illustrated as parts which are considered necessary for the description, and not all the information lines and the control lines are illustrated in the product. In fact, it is also contemplated that substantially all of the structures may be interconnected.

Description of the symbols

100-an automatic analyzer, 101-a sample container, 102-a sample disk, 103-a reagent container, 104-a reagent disk, 105-a sample dispensing mechanism, 106-a sample dispensing mechanism cleaning section, 107, 203, 303, 403, 503, 603-a reaction container, 108-a reaction container setting section, 109, 202, 302, 402, 502, 602-a reaction container transfer mechanism, 110-a reaction container supply section, 111-a detection unit, 112-a reagent dispensing mechanism, 113-a reagent dispensing mechanism cleaning section, 114-a reaction container discarding section, 115-an operation section, 116-a control section, 117-a storage section, 118-an interface, 119-a sample dispensing port, 201, 301, 401, 501, 601-a storage box, 204, 306, 406, 506, 606-a path of a reaction container transfer mechanism, 304, 504, 604-an opening section, 305, 405, 505, 605-a lid section, 404-an opening section, 507-a partition member, 607-probe, 800-automatic analyzer (modular type), 801-sample rack, 802-rack supply section, 803-rack storage section, 804-transport line (transport direction), 805-transport line (return direction), 806-rack standby section, 807-rack manipulation mechanism, 808-rack return mechanism, 809-rack distribution mechanism, 810-emergency sample rack input section, 811-reading section (transport line), 812-first blood coagulation analysis section, 813-first injection line, 814-first rack carry-in mechanism, 815-first rack manipulation mechanism, 816-reading section (first blood coagulation analysis section), 817-second blood coagulation analysis section, 818-second injection line, 819-second rack carry-in mechanism, 820-second rack manipulation mechanism, 821-reading section (second blood coagulation analysis section), 822-control section, 823-storage section, 824-output section, 825-input section, 826-first analysis unit, 827 — second analysis unit, 828, 829 — reaction vessel discarding unit, 830, 831 — reaction vessel transfer unit, 901 — reaction vessel, 902 — reaction vessel bank, 903 — reagent cartridge, 904 — reagent disk, 905 — reagent information reading unit, 906 — analysis port, 907 — analysis unit, 908 — preheating port, 909 — preheating unit, 910 — standby port, 911 — standby unit, 912 — reaction vessel transfer mechanism, 913 — reagent cartridge supply unit, 914 — reagent cartridge storage unit, 915 — reagent cartridge transfer mechanism, 916 — dispensed sample port, 917 — sample dispensing mechanism, 918 — sample dispensing mechanism cleaning unit, 919 — first reagent dispensing mechanism, 920 — first reagent dispensing mechanism cleaning unit, 921 — second reagent dispensing mechanism, 922 — second reagent dispensing mechanism cleaning unit, 923 — reaction vessel discarding unit.

Claims

1. An automatic analyzer is characterized by comprising:

a sample dispensing mechanism that dispenses a sample into a reaction vessel;

a reagent dispensing mechanism that dispenses a reagent into a reaction vessel;

a measuring unit including a reaction container holding unit for holding the reaction container, a light source for irradiating a mixed solution of a sample and a reagent contained in the held reaction container with light, and a detecting unit for detecting the irradiated light;

a reaction vessel discarding unit configured to discard the reaction vessel after the measurement is completed;

a reaction container transfer mechanism for transferring the reaction container from the reaction container holding portion to the reaction container discarding portion and discarding the reaction container to the reaction container discarding portion; and

a control unit for controlling the operation of the reaction vessel transfer mechanism,

the reaction vessel disposal unit includes: a storage unit for storing the discarded reaction vessel; and a cover portion formed to cover an upper surface of the receiving portion,

the lid portion is formed with an opening portion configured to include a plurality of disposal points into which the reaction vessel can be carried into the housing portion,

a detector for detecting the reaction container received through the disposal point is provided in the receiving portion,

the control unit controls the operation of the reaction container transfer mechanism based on a detection state of the reaction container by the detector so as to avoid continuous progress of a point where the reaction container is discarded in the reaction container discarding portion.

2. The automatic analysis device according to claim 1,

a plurality of opening portions are formed so as to correspond to the plurality of disposal points,

the control unit controls the operation of the reaction container transfer mechanism so as to prevent the reaction containers from being discarded continuously through any one of the plurality of openings, based on the detection state of the detector for the reaction containers stored through each of the plurality of openings.

3. The automatic analysis device according to claim 1,

the opening is formed singly so as to include a plurality of the disposal points,

the control unit controls the operation of the reaction container transfer mechanism so as to prevent the waste of the reaction container from being continuously performed through any one of the plurality of waste spots of the opening portion, based on a detection state of the detector with respect to the reaction container stored through each of the plurality of waste spots of the opening portion.

4. The automatic analysis device according to claim 2,

a plurality of detectors for detecting the reaction vessels accommodated in the accommodating section are provided in the accommodating section so as to correspond to the plurality of openings,

the controller controls the operation of the reaction container transfer mechanism based on a detection state of the reaction container by the detector.

5. The automatic analysis device according to claim 4,

in the case where at least any one of the plurality of detectors detects a reaction vessel,

the control unit controls the operation of the reaction container transfer mechanism so as to prevent the waste of the reaction containers from being continuously performed through the opening corresponding to the detector that detects the reaction containers.

6. The automatic analysis device according to claim 5,

as for the control section described above, it is preferable that,

storing position information of each of the plurality of probes and information on a timing of discarding at the corresponding opening,

when at least two or more of the detectors detect a reaction container, the deviation between the stored time for discarding at the corresponding opening and the current time is determined for each of the detectors, and the operation of the reaction container transfer mechanism is controlled so that the reaction container is discarded at the opening corresponding to the detector having the larger deviation.

7. An analysis method of an automatic analyzer, the automatic analyzer comprising:

a sample dispensing mechanism that dispenses a sample into a reaction vessel;

a reagent dispensing mechanism that dispenses a reagent into a reaction vessel;

the above-mentioned analytical method is characterized in that,