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CA2173862A1 - Apparatus and method for transfer of a fluid sample - Google Patents

Apparatus and method for transfer of a fluid sample

Info

Publication number
CA2173862A1
CA2173862A1 CA002173862A CA2173862A CA2173862A1 CA 2173862 A1 CA2173862 A1 CA 2173862A1 CA 002173862 A CA002173862 A CA 002173862A CA 2173862 A CA2173862 A CA 2173862A CA 2173862 A1 CA2173862 A1 CA 2173862A1
Authority
CA
Canada
Prior art keywords
reaction
nucleic acid
detection
chamber
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002173862A
Other languages
French (fr)
Other versions
CA2173862C (en
Inventor
Peter Zaun
Stanley R. Bouma
Julian Gordon
John J. Kotlarik
Natalie A. Solomon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/US1994/011016 external-priority patent/WO1995011437A1/en
Publication of CA2173862A1 publication Critical patent/CA2173862A1/en
Application granted granted Critical
Publication of CA2173862C publication Critical patent/CA2173862C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G01N2035/00366—Several different temperatures used
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G01N2035/00376—Conductive heating, e.g. heated plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G01N2035/00742—Type of codes
    • G01N2035/00752—Type of codes bar codes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device (16) that operates in conjunction with a reaction/detection unit (20). A sample (38) is loaded into a reaction chamber (30) of the device which is then mated with a detection chamber (32) to form the reaction/detection unit. A
first heating element (90) of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element (92) and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.
CA002173862A 1993-10-21 1994-09-28 Apparatus and method for transfer of a fluid sample Expired - Fee Related CA2173862C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14073093A 1993-10-21 1993-10-21
US08/140,730 1993-10-21
PCT/US1994/011016 WO1995011437A1 (en) 1993-10-21 1994-09-28 Apparatus and method for transfer of a fluid sample

Publications (2)

Publication Number Publication Date
CA2173862A1 true CA2173862A1 (en) 1995-04-27
CA2173862C CA2173862C (en) 2006-10-31

Family

ID=37395683

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002173862A Expired - Fee Related CA2173862C (en) 1993-10-21 1994-09-28 Apparatus and method for transfer of a fluid sample

Country Status (1)

Country Link
CA (1) CA2173862C (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110195017A (en) * 2019-06-11 2019-09-03 郑州大学第二附属医院 A kind of Parkinson's disease Disease-causing gene Mutation Screening detection method
CN110582703A (en) * 2017-02-07 2019-12-17 株式会社日立高新技术 Automatic analyzer
CN112129963A (en) * 2015-09-17 2020-12-25 株式会社日立高新技术 Automatic analysis device and analysis method

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112129963A (en) * 2015-09-17 2020-12-25 株式会社日立高新技术 Automatic analysis device and analysis method
CN110582703A (en) * 2017-02-07 2019-12-17 株式会社日立高新技术 Automatic analyzer
EP3581936A4 (en) * 2017-02-07 2020-12-23 Hitachi High-Tech Corporation Automatic analysis device
US11300488B2 (en) 2017-02-07 2022-04-12 Hitachi High-Tech Corporation Automatic analysis device
CN110582703B (en) * 2017-02-07 2023-01-06 株式会社日立高新技术 Automatic analyzer
CN110195017A (en) * 2019-06-11 2019-09-03 郑州大学第二附属医院 A kind of Parkinson's disease Disease-causing gene Mutation Screening detection method
CN110195017B (en) * 2019-06-11 2022-04-15 郑州大学第二附属医院 Parkinson disease pathogenic gene mutation screening and detecting method

Also Published As

Publication number Publication date
CA2173862C (en) 2006-10-31

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