CN111643411B

CN111643411B - Anti-aging composition and application thereof

Info

Publication number: CN111643411B
Application number: CN202010488887.9A
Authority: CN
Inventors: 方素云; 彭健; 胡晓珂; 闵军
Original assignee: Zhongke Marine Microbial Industry Technology Research Institute Shandong Co ltd; Yantai Institute of Coastal Zone Research of CAS
Current assignee: Zhongke Marine Microbial Industry Technology Research Institute Shandong Co ltd; Yantai Institute of Coastal Zone Research of CAS
Priority date: 2020-06-02
Filing date: 2020-06-02
Publication date: 2022-07-05
Anticipated expiration: 2040-06-02
Also published as: CN111643411A

Abstract

The invention relates to the field of skin care products, in particular to an anti-aging composition and application thereof, wherein the composition comprises the following raw materials in parts by weight: 5-15 parts of astaxanthin microcapsules, 5-10 parts of vaccinium myrtillus extracts, 5-10 parts of seaweed extracts, 2-5 parts of centella asiatica extracts and 2-5 parts of rosemary extracts. The anti-aging composition is rich in various active substances, such as astaxanthin, plant flavonoids, procyanidine, organic acid, alpha-hydroxy acid, beta-hydroxy acid, thiol enzyme and the like, and has good functions of scavenging free radicals, resisting oxidation, inhibiting bacteria, promoting skin metabolism and the like.

Description

Anti-aging composition and application thereof

Technical Field

The invention relates to the field of skin care products, in particular to an anti-aging composition and application thereof.

Background

The skin care product market is a monopoly competitive market, and the problem that many enterprises produce skin care products with different functions, whether the skin care products contain irritating chemical components and are harmful to the skin or not becomes the biggest problem when consumers buy the products. Many skin care products are illegally added with polysaccharide corticoids, and the skin care products containing the substances can cause skin thinning and telangiectasis hair follicle atrophy after long-term use; some skin care products contain not only glucocorticoids, but also a part of the skin care products contain a plurality of preservatives. These non-natural skin care products can cause skin irritation and even disfigurement. At present, the demand of people for skin care products is increasing day by day, and related data show that the market of the skin care products is developing rapidly at present, and the research on the efficacy of the skin care products is also an indispensable research focus for the development of the skin care product industry. For consumers, purchasing skin care products no longer only pays attention to price, but tends to be rational and more concerned about safety. Therefore, natural safety will be the dominant development of the skin care product industry for the future skin care product market.

The skin is the largest organ of the human body, and aging with age can cause the skin to be flaccid, rough and dull, wrinkles and spots to be deepened and become more. The skin aging is accompanied with the growth of half of a person, which has negative influence on the work and life of people who love beauty, and the anti-aging skin care products are greatly needed in the world, especially in affluent countries. With the economic progress of China, the skin care product industry develops rapidly. Under the background of the contemporary society, especially the aging of the population comes to make the anti-aging skin care products have wider development space. With the progress of biotechnology, the natural extract is applied to skin care products to realize the anti-aging function of the skin care products, which is a necessary trend for the development of the skin care product industry and is also a new demand for the skin care products at present.

Aiming at the characteristics of skin aging and the specific reasons for causing the skin aging, the invention improves the skin state by adding a plurality of active substances into the skin care product. The natural substances have obvious effects on anti-aging, whitening, moisturizing, sterilizing and the like, can be widely applied to various skin care products, and are hot spots for developing the skin care products.

The invention adopts astaxanthin as one of active components, but the astaxanthin is unstable and easy to oxidize and discolor due to the strong oxidation resistance, and the astaxanthin is also one of important factors limiting the application of the astaxanthin. According to the invention, the astaxanthin is microencapsulated, so that the stability is improved, the fishy smell of the astaxanthin is shielded, and the use comfort is improved.

Disclosure of Invention

The invention aims to provide an anti-aging composition and application thereof.

In order to achieve the purpose, the invention adopts the technical scheme that:

an anti-aging composition characterized by: the raw materials are as follows by weight: 5-15 parts of astaxanthin microcapsules, 5-10 parts of vaccinium myrtillus extracts, 5-10 parts of seaweed extracts, 2-5 parts of centella asiatica extracts and 2-5 parts of rosemary extracts.

The preferable raw materials comprise the following components in parts by weight: 7-12 parts of astaxanthin microcapsules, 6-9 parts of vaccinium myrtillus extracts, 6-9 parts of seaweed extracts, 3.5-4.5 parts of centella asiatica extracts and 3.5-4.5 parts of rosemary extracts.

Further preferably, the raw materials comprise the following components in parts by weight: 8-10 parts of astaxanthin microcapsules, 7-8 parts of vaccinium myrtillus extracts, 7-8 parts of seaweed extracts, 4-4.5 parts of centella asiatica extracts and 4-4.5 parts of rosemary extracts.

Mixing an astaxanthin extract with a wall material, performing ultrasonic emulsification at 30 ℃, adjusting the pH value of a mixed solution to 4.0, then cooling to 4 ℃, solidifying the microcapsule, and performing centrifugal separation to obtain the astaxanthin microcapsule;

mixing the astaxanthin extract with wall materials, performing ultrasonic emulsification at 30 ℃ for 20min, adjusting the pH value of the mixed solution to 4.0 by using 10% acetic acid, stirring at 400r/min for 30min, cooling to 4 ℃, adding a small amount of absolute ethyl alcohol to solidify microcapsules, and performing centrifugal separation to obtain the astaxanthin microcapsules.

The wall material is prepared by mixing gelatin, Arabic gum, hydroxypropyl methylcellulose and purified water according to a mass ratio of 1: 1: 0.2: 20, uniformly mixing and stirring to obtain a wall material;

the ratio (g/ml) of the astaxanthin extract to the wall material liquid is 5-10: 100.

The astaxanthin extract is extracted from haematococcus pluvialis, haematococcus pluvialis powder is weighed and placed in an extraction kettle, ethanol is added according to the feed-liquid ratio of 1:1g/ml, the temperature of the extraction kettle is set to be 40-42 ℃, the pressure is set to be 30Mpa, and CO is set₂The flow rate is 20L/h, the temperature of the first separation kettle is 30-35 ℃, the pressure is 8-9MPa, the temperature of the second separation kettle is 35-40 ℃, continuous dynamic extraction is carried out for 2h, dark red liquid is collected, and then the ethanol is removed by rotary evaporation, so that the astaxanthin extract is obtained.

The vaccinium myrtillus extract is prepared by mixing washed and crushed vaccinium myrtillus with water, adding mixed enzyme into the vaccinium myrtillus crushed mixed solution to adjust the pH value of the mixed solution to 4.0-5.0, placing the mixed solution in a water bath at 50-60 ℃ for enzymolysis for 2-4h, then adding sugar which accounts for 14% -20% of the mass of the mixed solution, inoculating mixed bacteria suspension into the adjusted enzymolysis product, taking supernatant after sealed fermentation, and carrying out reduced pressure ultrafiltration to obtain the vaccinium myrtillus extract concentrated solution.

The seaweed extract is prepared by cleaning and crushing seaweed, mixing the crushed seaweed with water, adding a mixed enzyme to adjust the pH value of a system to 4.5, carrying out enzymolysis for 2-4h in a water bath at 50-60 ℃, adding sugar accounting for 8-14% of the mass of the crushed mixed solution after enzymolysis, inoculating the adjusted enzymolysis product to the mixed bacterial suspension, carrying out sealed fermentation, taking supernatant, and carrying out reduced pressure ultrafiltration to obtain the seaweed extract concentrated solution.

The seaweed is selected from one or more of herba Zosterae Marinae, Undaria Pinnatifida and thallus laminariae.

In the preparation of the vaccinium myrtillus extract and the seaweed extract, the mixed enzyme is cellulase, hemicellulase, pectinase and acid protease, the addition amount of the cellulase is 0.2-0.6% of the mass of the mixed solution, the addition amount of the hemicellulase is 0.2-0.6% of the mass of the mixed solution, the addition amount of the pectinase is 0.16-0.22% of the mass of the mixed solution, and the addition amount of the acid protease is 0.2-0.6% of the mass of the mixed solution;

the mixed bacterial suspension is yeast suspension, lactobacillus plantarum bacterial suspension and bifidobacterium bacterial suspension, and the mass ratio of the enzymolysis product to the yeast bacterial suspension, the lactobacillus plantarum bacterial suspension and the bifidobacterium bacterial suspension is 50-100:1-2:1-2: 1-2.

The yeast suspension is 20% bacterial suspension prepared from yeast dry powder and sterile normal saline.

The lactobacillus plantarum bacterial suspension is a bacterial suspension which is prepared by 20% of lactobacillus plantarum dry powder with sterile normal saline.

The bifidobacterium bacterial suspension is a bacterial suspension prepared by 20 percent of bifidobacterium dry powder and sterile physiological saline.

The centella extract is prepared by cleaning fresh or dried centella, crushing, adding purified water and pectinase (the addition amount is 4mg/g) according to the material-liquid ratio of 1:45-60g/ml, carrying out enzymolysis for 2 hours at 45 ℃, carrying out hot reflux extraction for 2 hours, repeatedly extracting for 1 time, combining 2 times of filtrates, cooling the filtrate to room temperature, centrifuging for 15min at 10000r/min, obtaining a supernatant, and carrying out reduced pressure ultrafiltration to obtain the centella extract.

The rosemary extract is prepared by drying fresh rosemary at 40 ℃ for 10h, then crushing, adding purified water and pectinase (addition amount: 5mg/g) according to the material-liquid ratio of 1:40-60g/ml, carrying out enzymolysis at 45 ℃ for 2h, carrying out hot reflux extraction for 2h, repeatedly extracting for 1 time, combining 2 filtrates, cooling the filtrate to room temperature, centrifuging at 4 ℃, 10000r/min for 15min, obtaining a supernatant, and carrying out reduced pressure ultrafiltration to obtain the rosemary extract.

A skin care product comprises the anti-aging composition and an auxiliary agent, wherein the auxiliary agent is one or a mixture of a plurality of humectants, thickening agents, emulsifiers, emollients, chelating agents and deionized water.

The skin care product is one of facial mask, essence, lotion, skin care gel and cream.

Astaxanthin belongs to ketocarotenoid, and is extracted from food-grade Haematococcus pluvialis dry powder. Astaxanthin is a natural carotenoid, the capacity of scavenging free radicals is determined to be hundreds of times of that of vitamin E, oxidative stress injury can be prevented through various ways, and the astaxanthin has various biological effects of strong oxidation resistance, senility delaying, cancer resistance and the like. But the astaxanthin is unstable due to strong oxidation resistance, and the stability of the astaxanthin is improved by adopting a microcapsule technology, and meanwhile, the fishy smell of the astaxanthin is shielded, so that the use comfort is improved.

The effective components of Vaccinium myrtillus L.extract mainly comprise anthocyanidin, pectin, flavonoid, vitamins, minerals, etc. Especially contains rich anthocyanin, and has multiple physiological effects of scavenging free radicals, resisting inflammation, improving immunity, delaying aging, resisting cancer and the like.

The seaweed extract is a natural marine organism extract, contains alginic acid, crude protein, multiple vitamins, enzymes and trace elements, can reduce surface blood fat and improve skin hemopoiesis after being absorbed by skin, and has a large amount of anions which can stimulate fibroblasts to generate collagen and elastin, promote skin metabolism, resist wrinkles and resist aging.

The centella asiatica extract mainly comprises beta-sitosterol, hexacosanol caprylate, kaempferol, quercetin, daucosterol, vanillic acid, succinic acid, asiatic acid, madecassic acid and the like. The centella asiatica extract can tighten the connecting part of the epidermis and the dermis, can soften the skin and is beneficial to solving the skin relaxation phenomenon; help promote collagen formation in the dermis layer, allowing fibrin regeneration; can promote the close connection between epidermis and dermis, inhibit the increase of fat cells, and prevent skin edema.

The main functions of rosemary extract are astringing and antioxidation. The antioxidant components mainly comprise carnosic acid, carnosol, rosmanol, ursolic acid, rosmanic acid, etc.

The humectant is one or more of butanediol, glycerol, propylene glycol, sodium hyaluronate, betaine, biological sugar gum and polyethylene glycol.

The emollient is one or more of allantoin, hydrolyzed collagen, myristyl myristate, lauryl alcohol, and oleyl alcohol.

The emulsifier or thickener is one or more of xanthan gum, carbomer, sodium alginate and carboxypolymethylene.

The chelating agent is disodium EDTA.

The invention has the advantages that:

the anti-aging composition is rich in a plurality of active substances, such as astaxanthin, plant flavonoids, procyanidine, organic acid, alpha-hydroxy acid, beta-hydroxy acid, mercaptoenzyme and the like, and has good functions of scavenging free radicals, resisting oxidation, inhibiting bacteria, promoting skin metabolism and the like.

Astaxanthin with a strong antioxidant function is added into the anti-aging composition. Astaxanthin is the strongest antioxidant vitamin in the nature, has the reputation of 'super vitamin E', the antioxidant activity of the astaxanthin is 550 times of that of the vitamin E, and the astaxanthin can effectively protect the skin from being damaged by ultraviolet rays. It has strong ability of eliminating free radicals, and can prevent skin cells from being damaged by free radicals, and reduce wrinkle and freckle.

The astaxanthin added in the anti-aging composition is subjected to microencapsulation treatment, so that the problem that the astaxanthin is easily oxidized when meeting air is solved, and the tissue and activity of the astaxanthin are well protected. Astaxanthin can give off unpleasant taste, and is added after microencapsulation treatment, so that peculiar smell can be shielded, and comfort of users is improved.

The anti-aging composition disclosed by the invention is added with a plurality of natural extracts, contains a plurality of natural antioxidants, has different antioxidant mechanisms and action parts of different antioxidants, forms synergistic antioxidant effect by a plurality of modes of repairing, regenerating, absorbing oxygen, complexing metal ions and the like, forms a composite natural antioxidant after compatibility, simultaneously acts in cells and outside the cells, plays roles of each component, complements functions, enhances skin firmness, promotes cell metabolism, delays cell aging and even reverses aging, has excellent whitening and wrinkle removing effects, has the synergistic effect of a plurality of effective components, greatly improves the action effect of a single component, achieves the synergistic effect of multiple effects in one, is more efficient and higher in antioxidant performance compared with the single antioxidant, and can be used for masks, essence, emulsions, skin care gels and the like by matching different additives, Cream, etc.

The odorless plants are one of the most important sources of exogenous antioxidants of the human body, the components of the anti-aging composition are optimized, the proportion and the variety of natural substances are improved, the absorption of the skin to various active substances is enhanced, and the anti-aging composition has better use feeling and skin care effect. The anti-aging composition has the advantages of safe quality, no side effect and continuous effect, can further smooth the skin, remove skin aging wrinkles and achieve the purpose of delaying skin aging besides the basic moisturizing function of the skin care product containing the anti-aging composition, and is also the theoretical basis for the formation of the anti-aging skin care product.

The plant extract substances added in the anti-aging composition have the bacteriostatic effect, so that no chemical preservative can be added in the skin care product containing the anti-aging composition, and the safety is further improved.

The specific implementation mode is as follows:

the technical solution of the present invention is further illustrated below with reference to the following embodiments, and the described embodiments are only a part of the embodiments of the present invention, but the scope of the present invention is not limited thereto.

The astaxanthin microcapsules used in the following examples were prepared as follows:

mixing the astaxanthin extract with wall materials, performing ultrasonic emulsification at 30 ℃ for 20min, adjusting the pH value of the mixed solution to 4.0 by using 10% acetic acid, stirring at 400r/min for 30min, cooling to 4 ℃, adding a small amount of absolute ethyl alcohol until microcapsules are solidified, and performing centrifugal separation to obtain astaxanthin microcapsules;

the wall material is prepared by mixing gelatin, Arabic gum, hydroxypropyl methylcellulose and purified water according to the mass ratio of 1: 1: 0.2: mixing and stirring uniformly at a ratio of 20 to obtain a wall material;

the ratio of the astaxanthin extract to the wall materials (g/ml) is 8: 100.

The astaxanthin extract is extracted from haematococcus pluvialis, haematococcus pluvialis powder is weighed and placed in an extraction kettle, ethanol is added according to the feed-liquid ratio of 1:1g/ml, the temperature of the extraction kettle is set to be 40-42 ℃, the pressure is set to be 30Mpa, the flow rate of CO2 is set to be 20L/h, the temperature of a separation kettle is set to be 30 ℃, the pressure is set to be 8-9MPa, the temperature of a separation kettle is set to be 35 ℃, continuous dynamic extraction is carried out for 2h, dark red liquid is collected, and then the ethanol is removed through rotary evaporation, so that the astaxanthin extract is obtained.

The following examples employ a method of preparation of vaccinium myrtillus extract:

mixing the washed and crushed Vaccinium myrtillus with water, adding mixed enzyme into the crushed mixture of Vaccinium myrtillus to adjust pH value of the mixture to 4.5, placing in water bath at 55 deg.C for enzymolysis for 3h, adding sugar 16% of the mixture, inoculating mixed bacteria to the adjusted enzymolysis product, sealing, fermenting, collecting supernatant, and performing reduced pressure ultrafiltration to obtain Vaccinium myrtillus extract.

The seaweed extracts used in the following examples were prepared:

cleaning and crushing seaweed, mixing the seaweed with water, adding a mixed enzyme to adjust the pH value of a system to 4.5, carrying out enzymolysis for 4 hours in water bath at 55 ℃, adding sugar accounting for 12% of the mass of the crushed mixed solution after enzymolysis, inoculating the adjusted enzymolysis product into mixed bacterial suspension, carrying out sealed fermentation, carrying out ultrafiltration sterilization to obtain a primary fermentation liquid, taking supernatant, and carrying out reduced pressure ultrafiltration to obtain a seaweed extract. The seaweed material is kelp.

The mixed fungal enzymes in the preparation method of the vaccinium myrtillus extract and the seaweed extract are cellulase, hemicellulase, pectinase and acid protease, the addition amounts of the cellulase, the hemicellulase, the pectinase and the acid protease are respectively based on the mass ratio of the mixed solution, the addition amount of the cellulase is 0.4%, the addition amount of the hemicellulase is 0.4%, the addition amount of the pectinase is 0.2% and the addition amount of the acid protease is 0.4%.

The mixed bacterial suspension in the preparation method of the vaccinium myrtillus extract and the seaweed extract is yeast suspension, lactobacillus plantarum suspension and bifidobacterium suspension, and the enzymolysis product is uniformly stirred with the prepared yeast suspension, lactobacillus plantarum suspension and bifidobacterium suspension according to the mass ratio of 60:1.5:1.5: 1.5; the yeast suspension is 20% yeast suspension prepared from yeast dry powder and sterile normal saline; the lactobacillus plantarum bacterial suspension is a bacterial suspension with the concentration of 20 percent prepared by sterile normal saline for lactobacillus plantarum dry powder; the bacterial suspension of the bifidobacterium is 20 percent of bacterial suspension prepared by bifidobacterium dry powder and sterile normal saline.

The preparation of centella asiatica extract used in the following examples:

cleaning fresh or dried herba Centellae, pulverizing, adding purified water and pectase (addition amount: 4mg/g) at a ratio of 1:50g/ml, performing enzymolysis at 45 deg.C for 2 hr, performing heat reflux extraction for 2 hr, repeatedly extracting for 1 time, mixing filtrates, cooling the filtrate to room temperature, centrifuging at 4 deg.C and 10000r/min for 15min to obtain supernatant, and performing reduced pressure ultrafiltration to obtain herba Centellae extract.

The rosemary extracts used in the following examples were prepared:

drying fresh rosemary at 40 ℃ for 10h, then crushing, adding purified water and pectinase (the addition amount is 5mg/g) according to the material-liquid ratio of 1:40-60g/ml, carrying out enzymolysis at 45 ℃ for 2 hours, carrying out hot reflux extraction for 2 hours, repeatedly extracting for 1 time, combining 2 times of filtrates, cooling the filtrate to room temperature, centrifuging at 4 ℃ at 10000r/min for 15min, obtaining supernatant, and carrying out reduced pressure ultrafiltration to obtain the rosemary extract.

Other raw materials and equipment which are not specially described are all commercial products.

Example 1

The anti-aging composition comprises the following raw materials in parts by weight: 7 parts of astaxanthin microcapsules, 6 parts of vaccinium myrtillus extracts, 6 parts of seaweed extracts, 3.5 parts of centella asiatica extracts and 3.5 parts of rosemary extracts.

The anti-aging composition of this example was tested to have a DPPH radical scavenging capacity of 88.2%, superoxide anion radical scavenging capacity of 52%, and hydroxyl radical scavenging capacity of 23.9%; after the anti-aging composition is placed for half an hour, the moisture retention rate is 84.7 percent; has a certain inhibition rate on tyrosinase, and the inhibition effect is as follows: 38.5 percent.

The method for detecting the DPPH free radical scavenging capacity comprises the following steps: 1mL of the anti-aging composition mixture and 2mL of an 80% aqueous solution of DPPH in ethanol (2X 10) were sequentially added to a colorimetric tube⁴mol/L), fully oscillating and mixing uniformly, measuring the light absorption value at the wavelength of 517nm by using a 1cm cuvette, placing the cuvette in the dark for reacting for half an hour, immediately measuring the OD value at the wavelength after the reaction is finished, taking the OD value as a test group, and recording A₁(ii) a Mixing 2mL of the PPH solution and 1mL of 80% ethanol solution, measuring the absorbance under the same condition to obtain a blank group A₀(ii) a Mixing 1mL of the antiaging composition mixture with 2mL of 80% ethanol solution, measuring absorbance under the same condition, and recording as control group A₂. Each set of experiments was averaged in triplicate. DPPH radical clearance was calculated using the following formula: DPPH.radical scavenging ratio (%) [1- (A)₁-A₂)/A₀]×100。

The method for detecting the scavenging capacity of the superoxide anion free radicals comprises the following steps: 1mL of the anti-aging composition mixture and the control solution were weighed into a graduated tube, followed by addition of 0.5mL of phosphate buffer (0.5mol/L) at pH 7.8, addition of 1.5mL of methionine reagent (0.13mol/L), addition of 0.3mL of NBT solution (0.75mmol/L), and addition of 0.3mL of riboflavin (0.02mmol/L) to mix well, followed by reaction at 35 ℃ under 4000lx for 25 min. After the reaction, the reaction was terminated in the dark, and after the reaction was completed, the OD value was measured immediately at 560nm, and each test sample was recorded using distilled water as a blankOD value of (1). Each set of experiments was averaged in triplicate and recorded in detail. Superoxide anion clearance was calculated using the following equation: o is^2-Clearance (%) ═ a₁-A₂)/A₁X 100, wherein: a1 is the absorbance of the blank control, A2 is the absorbance of the mixture of the experimental composition.

The method for detecting the scavenging capacity of the hydroxyl free radical comprises the following steps: adding 1mL of the anti-aging composition mixed solution into a 10mL graduated test tube, taking 1mL of distilled water as a blank control, adding 1.0mL of hydrogen peroxide with the concentration of 6mmol/L, adding 1.0mL of salicylic acid with the concentration of 20mmol/L, and adding 0.5mL of ferrous sulfate with the concentration of 1.5 mmol/L. Heating in a thermostatic waterbath at 37 deg.C for 1h, adjusting to zero with distilled water, measuring absorbance A with spectrophotometer at wavelength of 562nm, and repeating for 3 times. The hydroxyl radical clearance is calculated using the following formula: hydroxyl radical scavenging rate (%) - (A)₁-A₂)/A₁]X 100, wherein: a. the₁Average absorbance for blank; a. the₂Is the average absorbance of the anti-aging composition mixture.

The moisture retention rate detection method comprises the following steps: placing the humectant in a dryer, and placing the allochroic silica gel at the bottom of the dryer. Soaking facial mask paper with a certain specification in the anti-aging composition mixed solution to uniformly soak the anti-aging composition mixed solution, taking out after 1h, weighing and counting, then placing the facial mask paper in a weighed beaker, uniformly placing the beaker close to the wall, then placing the beaker with the soaked facial mask paper in a dryer, placing the beaker in a safe and clean environment, and weighing after a certain period of time. In the test, the determination time interval is set as 0.5h, and then the moisture retention rate of the anti-aging composition mixed liquor is calculated. The calculation formula is as follows: moisture retention rate is M₂/M₁X 100%, wherein: m₁For the weight of water before standing, M₂Is the weight of water after standing.

Example 2

The anti-aging composition comprises the following raw materials in parts by weight: 8 parts of astaxanthin microcapsules, 7 parts of vaccinium myrtillus extracts, 7 parts of seaweed extracts, 4 parts of centella asiatica extracts and 4 parts of rosemary extracts.

The anti-aging composition of this example was tested to have a DPPH radical scavenging capacity of 91.3%, superoxide anion radical scavenging capacity of 55.2%, and hydroxyl radical scavenging capacity of 25.4%; after the anti-aging composition is placed for half an hour, the moisture retention rate is 86.2 percent; has a certain inhibition rate on tyrosinase, and the inhibition effect is as follows: 42.1 percent.

Example 3

The anti-aging composition comprises the following raw materials in parts by weight: 9 parts of astaxanthin microcapsules, 8 parts of vaccinium myrtillus extracts, 8 parts of seaweed extracts, 4.5 parts of centella asiatica extracts and 4.5 parts of rosemary extracts.

The anti-aging composition of this example was found to have a DPPH radical scavenging ability of 92.8%, superoxide anion radical scavenging ability of 58.6%, and hydroxyl radical scavenging ability of 27.2%; after the anti-aging composition is placed for half an hour, the moisture retention rate is 87.5 percent; has a certain inhibition rate on tyrosinase, and the inhibition effect is as follows: 43.5 percent.

Comparative example 1

The anti-aging composition comprises the following raw materials in parts by weight: 5 parts of bilberry extract, 5 parts of seaweed extract, 5 parts of asiatic centella extract and 5 parts of rosemary extract.

The anti-aging composition of this comparative example was tested to have a DPPH radical scavenging capacity of 65.2%, superoxide anion radical scavenging capacity of 39.5%, and hydroxyl radical scavenging capacity of 12.5%; after the anti-aging composition is placed for half an hour, the moisture retention rate is 78.6 percent; has a certain inhibition rate on tyrosinase, and the inhibition effect is as follows: 35.6 percent.

Comparative example 2

The anti-aging composition comprises the following raw materials in parts by weight: 5 parts of astaxanthin microcapsules, 5 parts of seaweed extract and 4 parts of centella asiatica extract.

The anti-aging composition of this comparative example was tested to have a DPPH radical scavenging capacity of 62.4%, superoxide anion radical scavenging capacity of 34.5%, and hydroxyl radical scavenging capacity of 10.6%; after the anti-aging composition is placed for half an hour, the moisture retention rate is 80.5 percent; has a certain inhibition rate on tyrosinase, and the inhibition effect is as follows: 22.6 percent.

Comparative example 3

The anti-aging composition comprises the following raw materials in parts by weight: 5 parts of astaxanthin extract, 4 parts of bilberry extract, 4 parts of seaweed extract, 2 parts of centella asiatica extract and 2 parts of rosemary extract.

The anti-aging composition of this comparative example was tested to have a DPPH radical scavenging capacity of 60.4%, superoxide anion radical scavenging capacity of 35.1%, and hydroxyl radical scavenging capacity of 9.6%; after the anti-aging composition is placed for half an hour, the moisture retention rate is 77.8 percent; has a certain inhibition rate on tyrosinase, and the inhibition effect is as follows: 20.5 percent.

As can be seen from the analysis of the above examples and comparative examples, the anti-aging composition obtained by the examples of the present invention has a good effect. The anti-aging composition of the embodiment has good anti-oxidation activity effect and can play a role in repairing cells and delaying aging in view of the DPPH free radical scavenging capacity, superoxide anion free radical scavenging capacity and hydroxyl free radical scavenging capacity. The whitening effect of the anti-aging composition is proved by detecting the tyrosinase activity inhibition rate, the obtained embodiment has high tyrosinase inhibition rate which can reach 43.5%, and the anti-aging composition is proved to have the whitening effect at the same time.

The anti-aging composition ingredients of comparative examples 1 and 2 lack 1-2 ingredients, and the DPPH radical scavenging ability, superoxide anion radical scavenging ability, hydroxyl radical scavenging ability, and tyrosinase inhibition rate were significantly lower than those of the examples. This is because different antioxidants have different mechanisms and functional sites during their use, and they have synergistic effects and cannot be substituted for each other, and the antioxidant properties are limited when the antioxidant structure is relatively single. The invention has synergistic effect among different types of antioxidants, the characteristics of all components are complementary, the coordination and the synergy are realized, the oxidation resistance is obviously improved, and the invention has stronger anti-aging performance.

The anti-aging composition of comparative example 3, in which non-microencapsulated astaxanthin was used, was remarkably reduced in oxidation resistance because astaxanthin, although having strong oxidation resistance, is very unstable and easily oxidatively denatured upon contact with air, while microencapsulation improved stability and water solubility of astaxanthin.

The composition obtained in the embodiment can be prepared into one of facial masks, essence, lotion, skin-care gel and cream according to the existing preparation method of cosmetics and the selection of corresponding auxiliary agents.

Application example 1

The mask containing the anti-aging composition of example 1 further comprises an auxiliary agent, wherein the auxiliary agent comprises 5 parts of butanediol, 4 parts of glycerol, 3 parts of propylene glycol, 1.5 parts of betaine, 0.8 part of bioglycan, 0.8 part of 1, 2-pentanediol, 1.5 parts of sodium hyaluronate, 0.2 part of allantoin, 1 part of hydrolyzed collagen, 0.03 part of disodium EDTA, and the balance of deionized water to 100 parts.

The preparation method of the mask containing the anti-aging composition comprises the following steps:

(1) adding water, butanediol, glycerol, propylene glycol, betaine, bioglycan gum, 1, 2-pentanediol, sodium hyaluronate, allantoin, hydrolyzed collagen and EDTA disodium into a reactor in sequence according to the weight parts, heating to 80-85 ℃, stirring until the materials are completely dissolved, and preserving heat for 20min to obtain a mixed solution.

(2) And (2) stirring the mixed solution prepared in the step (1), cooling to 30-35 ℃, adding the anti-aging composition according to the weight parts, uniformly stirring, and standing for 10 hours to obtain the facial mask nutrient solution.

(3) Filling the facial mask nutrient solution into a packaging bag containing facial mask paper after irradiation disinfection to obtain the facial mask containing the anti-aging composition.

Application example 2

The mask containing the anti-aging composition of example 2 further comprises an auxiliary agent, wherein the auxiliary agent comprises 6.5 parts of butanediol, 6 parts of glycerol, 5 parts of propylene glycol, 2 parts of betaine, 1.1 parts of bioglycan, 1.1 parts of 1, 2-pentanediol, 2.5 parts of sodium hyaluronate, 0.3 part of allantoin, 2 parts of hydrolyzed collagen, 0.04 part of disodium EDTA, and the balance of deionized water to 100 parts.

The preparation method of the facial mask containing the anti-aging composition is the same as the application example 1.

Application example 3

The mask containing the anti-aging composition of example 3 further comprises an auxiliary agent, wherein the auxiliary agent comprises 6 parts of butanediol, 5 parts of glycerol, 4 parts of propylene glycol, 2 parts of betaine, 1.1 parts of bioglycan, 1.1 parts of 1, 2-pentanediol, 2.5 parts of sodium hyaluronate, 0.3 part of allantoin, 2 parts of hydrolyzed collagen, 0.04 part of disodium EDTA, and the balance of deionized water to 100 parts.

Application example 4

The essence containing the anti-aging composition of example 3 further includes an auxiliary agent, which is 7 parts of sodium hyaluronate, 2 parts of glycerin, 1.5 parts of bioglycan, 3 parts of polyethylene glycol, 0.3 part of allantoin, 2 parts of hydrolyzed collagen, 0.4 part of xanthan gum, 0.04 part of disodium EDTA, and the balance up to 100 parts of deionized water.

The preparation method of the essence containing the anti-aging composition comprises the following steps:

(1) adding water, glycerol, bioglycan, polyethylene glycol, allantoin, hydrolyzed collagen, xanthan gum and EDTA disodium into a reactor in sequence according to the weight parts, heating to 80-85 ℃, stirring until the materials are completely dissolved, and preserving heat for 20min to obtain a mixed solution.

(2) And (2) stirring the mixed solution prepared in the step (1), cooling to 30-35 ℃, adding sodium hyaluronate according to the weight parts, uniformly stirring the anti-aging composition, and standing for 10 hours to obtain the anti-aging essence.

Application example 5

The skin care gel containing the anti-aging composition of example 3 further comprises an auxiliary agent, wherein the auxiliary agent is 7 parts of sodium hyaluronate, 1 part of glycerol, 3 parts of polyethylene glycol, 0.3 part of allantoin, 0.02 part of sodium alginate, 0.01 part of carbomer, 0.04 part of disodium EDTA, and the balance of deionized water to 100 parts.

The preparation method of the skin care gel containing the anti-aging composition comprises the following steps:

(1) adding sodium hyaluronate, glycerol, polyethylene glycol, an anti-aging composition and a proper amount of deionized water according to the weight parts, and uniformly stirring to obtain a solution 1.

(2) And weighing the sodium alginate in parts by weight, and dispersing the sodium alginate in a proper amount of deionized water to form gel liquid 1.

(3) And weighing the carbomer in parts by weight, and dispersing the carbomer into a proper amount of deionized water to form gel liquid 2.

(4) And respectively adding the solution 1, the gel solution 2 and the EDTA disodium into the balance of deionized water, and fully stirring and uniformly mixing to obtain the anti-aging skin care gel.

Sensory evaluation of mask

20 volunteers were selected by a double-blind random method, and after skin was cleaned, 1 piece of the facial mask of application example 1 was spread and attached to the face, and after 15 minutes, was peeled off and washed clean with clear water. Sensory evaluation volunteers claim: the human body is healthy after being aged 25-55 years, volunteers can accurately express the use feelings after being trained, and the evaluation standard is shown in Table 1.

Table 1: sensory evaluation index of facial mask

Table 2: comprehensive evaluation result table of facial mask

Sample evaluation	Example 1	Example 2	Example 3	Comparative example 1	Comparative example 2
						Total score	88	92	93	80	78

The facial mask liquid disclosed by the invention is good in color, light purple, light in delicate fragrance, free of peculiar smell, fine and smooth, free of tight, mild and irritation to the face after being used, comfortable and cool in feeling, free of greasy adhesion, free of any adverse reaction and good in safety.

The astaxanthin microencapsulation can shield peculiar smell and improve stability and water solubility. Besides the functions of antioxidation, whitening and anti-aging, the natural plant extract has the effects of bacteriostasis and corrosion prevention, avoids the addition of chemical preservatives and further ensures the pure naturalness of the mask liquid.

Sensory evaluation of mask for anti-aging

Compared with other skin care products, the facial mask has the obvious characteristics of rapidly supplementing moisture and nutrient substances to the skin and having good instant effect. Therefore, the test adopts a double-blind random test method to evaluate the human body efficacy, a multifunctional skin tester is used for measuring the skin state parameters (including the skin water content and the skin elasticity) of the volunteer before use (0 h) and after use (4 h and 8h), the skin water content and the elasticity value change value are obtained by subtracting the value before use from the detection value after use, and the effect is better when the value is larger.

The using method comprises the following steps: after skin cleaning, 1 piece of the facial mask of application example 1 is spread and pasted on a face, and is taken off after 15 minutes, and is washed clean by clear water.

The volunteer requires: 30 people, age 25-55 years old, healthy, volunteers trained, can express their own use experience accurately.

TABLE 3 anti-aging sensory evaluation results of facial mask

	Example 1	Example 2	Example 3	Comparative example 1	Comparative example 2
						Skin moisture content (0-4h)	26.5	29.6	32.5	18.8	16.9
Skin moisture content (0-8h)	15.6	17.8	20.2	10.3	9.8
						Skin elasticity value (0-4h)	18.6	20.7	22.5	9.5	8.8
Skin elasticity value (0-8h)	9.2	9.5	10.6	2.6	2.1

As can be seen from the above table, after the facial mask is used for 4 hours and 8 hours, the moisture content and elasticity value of the skin of the experimental group and the control group are greatly improved after the product of the invention is used, and the moisture content and elasticity value of the skin fall back to 8 hours, which indicates that the prepared facial mask has good effect of improving the moisture content and elasticity of the skin and the duration time is longer than 8 hours. Meanwhile, the embodiment is more improved compared with the comparative example, and the falling speed is slow, so that the added astaxanthin and various natural plant extracts have the obvious effects of improving the moisture content and the elasticity value of the skin. Experiments show that the facial mask prepared by adding the astaxanthin and other plant extracts has the obvious effects of improving the water content and the elasticity of the skin, and no one of all volunteers has the symptoms of skin allergy and the like.

The anti-aging composition disclosed by the invention has the advantages that the anti-oxidation activity is obviously enhanced after the combination of various natural substances, and the anti-aging composition prepared by the invention achieves a remarkable anti-oxidation effect by forming a synergistic anti-oxidation effect in various modes such as repairing, regenerating, oxygen absorbing, metal ion complexing and the like just because various substances such as astaxanthin, blueberry extract, seaweed extract and the like have different anti-oxidation action mechanisms, and has the effects of obviously removing fine wrinkles and improving the skin elasticity. Therefore, the anti-aging composition disclosed by the invention can generate a good effect of immediately improving skin, can promote cell metabolism, is natural and non-irritant in the added raw materials, and can be widely applied to anti-aging skin care products.

Claims

1. An anti-aging composition characterized by: the raw materials comprise the following components in parts by weight: 5-15 parts of astaxanthin microcapsules, 5-10 parts of vaccinium myrtillus extracts, 5-10 parts of seaweed extracts, 2-5 parts of centella asiatica extracts and 2-5 parts of rosemary extracts;

the vaccinium myrtillus extract is prepared by mixing washed and crushed vaccinium myrtillus with water, adding mixed enzyme into the vaccinium myrtillus crushed mixed solution to adjust the pH value of the mixed solution to 4.0-5.0, placing the mixed solution in a water bath at 50-60 ℃ for enzymolysis for 2-4h, then adding sugar which accounts for 14% -20% of the mass of the mixed solution, inoculating mixed bacteria suspension into the adjusted enzymolysis product, taking supernate after sealed fermentation, and carrying out reduced pressure ultrafiltration to obtain the vaccinium myrtillus extract;

the seaweed extract is prepared by cleaning and crushing seaweed, mixing the crushed seaweed with water, adding a mixed enzyme to adjust the pH value of a system to 4.5, carrying out enzymolysis for 2-4h in a water bath at 50-60 ℃, adding sugar accounting for 8-14% of the mass of the crushed mixed solution after enzymolysis, inoculating the adjusted enzymolysis product to a mixed bacteria suspension, carrying out sealed fermentation, taking supernatant, and carrying out reduced pressure ultrafiltration to obtain the seaweed extract;

the mixed enzyme is cellulase, hemicellulase, pectinase and acid protease, the addition amount of the cellulase is 0.2-0.6% of the mass of the mixed solution, the addition amount of the hemicellulase is 0.2-0.6% of the mass of the mixed solution, the addition amount of the pectinase is 0.16-0.22% of the mass of the mixed solution, and the addition amount of the acid protease is 0.2-0.6% of the mass of the mixed solution;

the mixed bacteria suspension is yeast bacteria suspension, lactobacillus plantarum suspension and bifidobacterium suspension, and the mass ratio of the enzymolysis product to the yeast bacteria suspension, the lactobacillus plantarum suspension and the bifidobacterium suspension is 50-100:1-2:1-2: 1-2.

2. The anti-aging composition according to claim 1, wherein: the raw materials are as follows by weight: 7-12 parts of astaxanthin microcapsules, 6-9 parts of vaccinium myrtillus extracts, 6-9 parts of seaweed extracts, 3.5-4.5 parts of centella asiatica extracts and 3.5-4.5 parts of rosemary extracts.

3. The anti-aging composition according to claim 2, wherein: the raw materials are as follows by weight: 8-10 parts of astaxanthin microcapsules, 7-8 parts of vaccinium myrtillus extracts, 7-8 parts of seaweed extracts, 4-4.5 parts of centella asiatica extracts and 4-4.5 parts of rosemary extracts.

4. The anti-aging composition according to claim 3, wherein: the astaxanthin microcapsule is prepared by mixing an astaxanthin extract with a wall material, then carrying out ultrasonic emulsification, adjusting the pH value of a mixed solution to 4.0, cooling and solidifying the microcapsule, and carrying out centrifugal separation;

the ratio of the astaxanthin extract to the wall material liquid is 5-10:100 g/ml.

5. A skin care product characterized by: the skin care product comprises the anti-aging composition of any one of claims 1 to 4 and an adjuvant.

6. A skin care product according to claim 5 wherein: the auxiliary agent is one or a mixture of a plurality of humectants, thickening agents, emulsifiers, emollients, chelating agents and deionized water.

7. A skin care product according to claim 5 wherein: the skin care product is one of facial mask, essence, lotion, skin care gel and cream.