CN110917061B - Composition containing ergothioneine extracting solution, brown rice fermentation filtrate and acetyl chitosamine and application thereof - Google Patents
Composition containing ergothioneine extracting solution, brown rice fermentation filtrate and acetyl chitosamine and application thereof Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61Q19/08—Anti-ageing preparations
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Abstract
The invention discloses a composition containing ergothioneine extracting solution, brown rice fermentation filtrate and acetyl chitosamine and application thereof, wherein the ergothioneine extracting solution is 0.1-2.0 parts, the brown rice fermentation filtrate is 0.1-4.0 parts, and the acetyl chitosamine is 0.1-2.0 parts. The ergothioneine extracting solution, the brown rice fermentation filtrate and the acetyl chitosamine are selected as three components of natural fermentation sources, so that the ergothioneine extracting solution, the brown rice fermentation filtrate and the acetyl chitosamine have the advantages of being natural, high in activity, easy to absorb and high in safety, obvious synergistic effect is shown by screening of dosage relation of the components, and the effects of regulating biological rhythm of skin, delaying skin aging and improving pigmentation are achieved.
Description
Technical Field
The invention relates to a composition, in particular to a composition containing ergothioneine extract, brown rice fermentation filtrate and acetyl chitosamine, and application of the composition in the aspects of regulating biological rhythm and resisting blue light, belonging to the technical field of cosmetics.
Background
The solar spectrum can be roughly divided into three parts: ultraviolet (wavelength 200-400 nm), visible (wavelength 400-800 nm) and infrared (wavelength 800nm-1 mm). Awareness of the hazards of excessive exposure to ultraviolet light has been increasingly heightened. Compared with ultraviolet rays, visible light has stronger penetrating power and can reach the dermis of human skin directly, so that the visible light can cause deeper damage to the skin and even genes. The visible light is composed of seven colors of light such as purple, blue, cyan, green, yellow, orange, red and the like, wherein the blue light wave band with the wavelength of 400-500nm has the highest energy, and the harm to human bodies is the greatest. Studies have shown that irradiation of human skin in the 280-700 nm band produces free radicals of which 50% are from the UVA band and 50% are from the visible band, of which about 35% are from the blue band, and thus the visible blue light is very harmful to the skin.
Besides the existence of 90% of sunlight, blue light also widely exists in electronic products, and computer displays, fluorescent lamps, mobile phones, digital products, display screens, LEDs and the like are almost surrounded by the blue light all the time. The damage of blue light to the skin is mainly manifested by inducing pigmentation, and accelerating skin aging and relaxation. Blue light can penetrate into the dermis of the skin, under the irradiation of the blue light, mitochondria in cells can generate active oxygen free radicals, and the active oxygen free radicals can generate oxidation reaction with the mitochondria, DNA and cell structures containing lipid, such as cell membranes, mitochondrial membranes, lysosomes and the like, so that the mitochondria and the DNA are damaged, the cell membrane dysfunction is caused, the cell growth and development are influenced, the cell structures are damaged, and the cell apoptosis is caused by the cell damage. Apparently, blue light induces the production of free radicals by cells, causing the fibroblasts of the dermal layer of the skin to be damaged and the extracellular matrix to be reduced, thereby causing premature aging of the skin. In addition, blue light is a stimulation source of biological rhythms, and the long-term overexposure to the blue light environment is easy to break the biological rhythms of cells, so that the skin is in a fatigue state.
For blue light, the following techniques are generally used in skin care products: firstly, physical protection raw materials such as mica, pearl powder, boron nitride and the like are adopted to directly block and reflect blue light; secondly, radiation-absorbing components are used, and this technique generally uses blue-light absorbers, such as toner or some high molecular materials, and in addition, lutein, coffee beans and the like of plant origin can also absorb radiation; thirdly, with pigmentation-improving ingredients, studies have shown that the pigmentation residue induced by BLUE light is more permanent than that induced by UVB, and in cosmetics several antioxidants are generally used to achieve the effect of improving pigmentation, such as sarcophylin (INCI name carnosine) by deluxe, BLUE oeococtif by OLEOS, france; fourthly, regulating biological rhythm components which can restore the normal physiological rhythm of the skin, enhancing the resistance of the skin in a healthy state, and having better functions of resisting aging, resisting pigmentation and resisting free radicals; fifthly, ingredients for delaying skin aging are adopted, the skin carries a photosensitive system to visible rays, the skin senses blue light through opsin photoreceptors on keratinocytes and melanocytes, opsin participates in epidermal barrier differentiation and clock signal synchronization, excessive exposure to the blue light can cause opsin aggregation in cells, cell damage is caused, excessive exposure to the blue light can delay barrier repair, and the ingredients for delaying skin aging can improve barrier repair capability.
CN110236965A discloses an anti-blue light skin care cosmetic and a preparation method thereof, wherein the anti-blue light composition comprises carotene, flos Camelliae Japonicae extract, and stigma croci Sativi extract, and the composition can improve pigmentation by absorbing radiation and resisting oxidation to achieve anti-blue light effect, and has no effect of regulating biological rhythm.
CN110292547A discloses a cosmetic composition, a preparation method thereof and a cosmetic with blue light resistance, wherein the composition is mainly prepared from seawater, palmitoyl tripeptide-5, an algae extract, hesperidin methyl chalcone, a cocoa seed extract, carnosine, caffeine, an anti-allergy agent, a chelating agent, water and other raw materials, and the components are complex.
CN106727027A discloses a composition for resisting blue light, resisting oxidation and scavenging free radicals, a skin care product and a preparation method thereof, wherein the composition comprises a compound of marigold flower extract and safflower seed oil, a tea extract and an alpine edelweiss extract, and the blue light resisting functional component of the composition is mainly the compound of the marigold flower extract and the safflower seed oil, can supplement lutein, achieves the blue light resisting effect by absorbing radiation, and has no biological rhythm regulating effect.
At present, the blue light resisting compositions on the market are various in types, but the combination modes and the effects of raw materials are different, and no composition for realizing the blue light resisting effect from three aspects of improving pigmentation, regulating biological rhythm and delaying aging is seen at present.
Disclosure of Invention
Based on the above, the invention aims to provide the composition containing the ergothioneine extracting solution, the brown rice fermentation filtrate and the acetyl chitosamine, the composition has few raw material types, has the characteristics of regulating biological rhythm, improving pigmentation and delaying skin aging, and has better blue light resisting effect by combining the three effects in a synergistic manner.
The ergothioneine, the brown rice fermentation filtrate and the acetyl chitosamine are selected as effective components, the components have the advantages of naturalness, high activity, easy absorption and high safety, the combination of the components can regulate the skin state in three aspects of regulating biological rhythm, improving pigmentation and delaying skin aging, and particularly the composition has the function of regulating the biological rhythm, can improve the fatigue state of the skin, improve the health condition of the skin, improve the resistance of the skin to the aging and pigmentation problems caused by blue light and improve the blue light resistance of the skin.
In order to realize the purpose, the technical scheme adopted by the invention is as follows:
a composition containing ergothioneine extract, brown rice fermentation filtrate and acetyl chitosamine comprises the following effective components in parts by weight: 0.1-2.0 parts of ergothioneine extract, 0.1-4.0 parts of brown rice fermentation filtrate and 0.1-2.0 parts of acetyl chitosamine.
Preferably, the composition containing the ergothioneine extracting solution, the brown rice fermentation filtrate and the acetyl chitosamine comprises the following effective components in parts by weight: 0.5-2.0 parts of ergothioneine extract, 0.5-4.0 parts of brown rice fermentation filtrate and 0.5-2.0 parts of acetyl chitosamine.
Furthermore, ergothioneine (L-Ergothionine, EGT) is chemically named as 2-sulfydryl histidine trimethyl inner salt, is the only known natural 2-thioimidazole amino acid so far, and has definite antioxidant and anti-inflammatory effects. At present, many methods for preparing ergothioneine are reported, including a chemical synthesis method, an extraction method and a biological fermentation synthesis method, and the fermentation method is low in cost, convenient for industrial production and the main production method at present. The ergothioneine extracting solution used by the invention is prepared by a fermentation method, the content of the ergothioneine in fermentation liquids obtained by different fermentation methods is different, and the extracting solution with high content of the ergothioneine is preferably selected by the invention.
Preferably, the ergothioneine extract of the invention is prepared from Hericium erinaceus (Hericium erinaceus) CCTCC No: m2018567, and is prepared by fermenting Hericium erinaceus (Hericium erinaceus) with a culture medium with a preservation number of CCTCC No: treating fermentation liquor obtained by fermentation of M2018567 to obtain ergothioneine extract, wherein the specific method comprises the following steps: hericium erinaceus (Hericium erinaceus) CCTCC No: inoculating the M2018567 mycelium into a liquid seed culture medium for culture to obtain a seed solution; inoculating the seed liquid into a fermentation culture medium for fermentation, adding a ergothioneine precursor substance, and fermenting to the end point to obtain a fermentation liquid; and (3) treating the fermentation liquor containing the mycelium to obtain an ergothioneine extracting solution. The method is described in detail in CN201811605003.2 and CN 201910157074.9. The ergothioneine extract contains L-ergothioneine, dextran, polypeptide, amino acid, polysaccharide, etc., has good antioxidant effect, and also has effects of protecting DNA of skin cells, reducing apoptosis, protecting cells from ultraviolet injury, scavenging free radicals, and preventing pigmentation.
Furthermore, the content of the ergothioneine in the ergothioneine extracting solution is more than or equal to 200mg/L, and preferably more than or equal to 300 mg/L. The higher the ergothioneine content, the stronger the effect of regulating biological rhythm, improving pigmentation and delaying skin aging and the better the blue light resistance effect by combining with the brown rice fermentation filtrate and the acetyl chitosamine.
Further, the brown rice fermentation filtrate is obtained by performing impurity removal and sterilization treatment on fermentation liquor obtained by fermenting brown rice with yeast, and the more specific preparation method comprises the following steps: accelerating germination of brown rice, crushing the brown rice into brown rice powder, preparing the brown rice powder into rice pulp, performing enzymolysis on the rice pulp, adding yeast for fermentation, and removing impurities and bacteria from fermentation liquor to obtain brown rice fermentation filtrate. The brown rice is whole grain rice with complete vitality, contains rich dietary fiber, functional active ingredients and mineral substances, and can improve cell activity and human body immunity. Fermenting brown rice with yeast, fully releasing, converting and enriching nutrients in the fermented brown rice, wherein the obtained brown rice fermentation filtrate contains rich active ingredients such as gamma-aminobutyric acid, polypeptide, amino acid, active oxygen resistant phytic acid, ferulic acid, lactic acid, plant amide and sterol, and the amino acid types comprise more than 20 of hydroxyproline, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, ornithine, lysine, histidine, tryptophan and arginine. The active ingredients can improve cell activity, promote cell regeneration and tissue repair, improve skin darkness, and make skin moist and beautiful. The brown rice fermentation filtrate mainly has the effects of improving cell activity, promoting cell regeneration and tissue repair, resisting oxidation, delaying aging and preventing pigmentation (compared with the commonly used freckle removing agent). The brown rice fermentation filtrate is a product prepared by the applicant, is a commercial product at present, and the content of gamma-aminobutyric acid in the product is as high as 1g/L.
Furthermore, the chemical name of the acetylcysteine amine is N-acetyl-D-glucosamine (NAG), the safe and mild small molecular amino monosaccharide is obtained by selecting plant-derived fermentation raw materials and adopting non-transgenic safe strains for fermentation, the safe and mild small molecular amino monosaccharide HAS excellent transdermal absorbability, can improve the hydration of the skin, is a high-quality multifunctional humectant, and can enhance the activity of Hyaluronic Acid Synthase (HAS), promote the synthesis and secretion of hyaluronic acid by skin keratinocytes and fibroblasts and improve the content of hyaluronic acid in the skin. The acetyl chitosamine can also remove cutin and maintain normal metabolism of surface layer keratinocyte, unhealthy keratinocyte metabolism makes its surface glycoprotein intertwined together, and is not easy to shrink into small dandruff and shed, NAG can normalize metabolism of keratinocyte surface glycoprotein, and make stratum corneum pushed to outermost layer easily form tiny scale shape and naturally shed. In addition, the synergistic effect of NAG and nicotinamide can reduce local pigmentation, lighten skin color spots, reduce the phenomena of skin color deepening and uneven skin color caused by ultraviolet rays and effectively homogenize the skin color.
The composition is simple to prepare, and can be prepared by uniformly mixing the components, wherein the obtained composition is in a liquid state and needs to be stored in a dark place.
As can be seen from the above descriptions of the functional effects of ergothioneine extract, brown rice fermentation filtrate and acetyl chitosamine, the effects of the components reported in the prior art are antioxidant, moisturizing, anti-aging and pigmentation preventing effects, and no related report on the effect of regulating biological rhythm is found. The experiment proves that the composition not only has the functions of resisting oxidation, preventing aging and preventing pigmentation, but also can regulate biological rhythm, improve the recovery capability of cells and have better blue light resistance effect.
Further, the invention also provides application of a composition containing ergothioneine extract, brown rice fermentation filtrate and acetyl chitosamine in preparing cosmetics with the effects of regulating skin biological rhythm, delaying skin aging or improving pigmentation.
Further, the invention also provides application of the composition containing the ergothioneine extracting solution, the brown rice fermentation filtrate and the acetyl chitosamine in blue light resisting cosmetics.
Further, the present invention provides a cosmetic comprising the above-mentioned ergothioneine-containing extract, brown rice fermented filtrate and chitosamine as effective ingredients, which may be used alone or in combination with other ingredients having different effects. Preferably, the cosmetic is a blue light resistant cosmetic.
Further, the content of ergothioneine extract in the cosmetic is 0.1-2.0wt%, the content of brown rice fermentation filtrate in the cosmetic is 0.1-4.0 wt%, and the content of acetylcysteine in the cosmetic is 0.1-2.0wt%.
Further, the cosmetic comprises, besides the active ingredients, matrix ingredients necessary for preparing different types of cosmetics, wherein the matrix ingredients include, but are not limited to, solvents, humectants, emollients, emulsifiers, preservatives, essences and the like. The cosmetics may be in the form of lotions, essences, milks, creams, masks, introductions, color cosmetics, and the like. Depending on the type of cosmetic product, the required base composition may vary.
The ergothioneine extracting solution, the brown rice fermentation filtrate and the acetyl chitosamine are selected as three components of natural fermentation sources, so that the ergothioneine extracting solution, the brown rice fermentation filtrate and the acetyl chitosamine have the advantages of being natural, high in activity, easy to absorb and high in safety, obvious synergistic effect is shown by screening of dosage relation of the components, and the effects of regulating biological rhythm of skin, delaying skin aging and improving pigmentation are achieved.
Detailed Description
The present invention will be further described with reference to specific examples for better illustrating the objects, technical solutions and advantages of the present invention. It should be noted that the process parameters, raw materials and the like which are not described in detail in the present invention are performed according to the conventional technical means in the art.
In the following examples, the ergothioneine extract was obtained from Huaxi Biotech Co., ltd and is commercially available as ergothioneine Armillaria matsutake extract (Bioyouth. RTM. -EGT), and the content of ergothioneine was 200mg/L or more. The brown rice fermentation filtrate used is also available from Huaxi Biotech Co., ltd, which is commercially available under the name brown rice fermentation filtrate. The acetyl chitosamine used is also from Huaxi Biotech Co., ltd, the product being marketed under the name Hyafactor TM -NAG acetylchitosamine.
Example 1
The composition comprises the following components in parts by weight: 1.0 part of ergothioneine pine mushroom extract, 2.0 parts of brown rice fermentation filtrate and 1.0 part of acetyl chitosamine.
The preparation method comprises the following steps: dissolving acetyl chitosamine in ergothioneine Armillaria mellea extract and brown rice fermentation filtrate, mixing, stirring, and dissolving completely to obtain composition, and storing in dark place.
Example 2
The composition comprises the following components in parts by weight: 0.1 part of ergothioneine pine mushroom extract, 4.0 parts of brown rice fermentation filtrate and 0.2 part of acetyl chitosamine.
The preparation method is the same as example 1.
Example 3
The composition comprises the following components in parts by weight: 0.5 part of ergothioneine pine mushroom extract, 1.0 part of brown rice fermentation filtrate and 2.0 parts of acetyl chitosamine.
The preparation method is the same as example 1.
Example 4
The composition comprises the following components in parts by weight: 2.0 parts of ergothioneine pine mushroom extract, 4.0 parts of brown rice fermentation filtrate and 0.5 part of acetyl chitosamine.
The preparation method is the same as example 1.
Example 5
The composition comprises the following components in parts by weight: 1.5 parts of ergothioneine pine mushroom extract, 0.1 part of brown rice fermentation filtrate and 1.5 parts of acetyl chitosamine.
The preparation method is the same as example 1.
Example 6
The composition comprises the following components in parts by weight: 1.0 part of ergothioneine pine mushroom extract, 3.0 parts of brown rice fermentation filtrate and 1.0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Example 7
The composition comprises the following components in parts by weight: 2.0 parts of ergothioneine pine mushroom extract, 4.0 parts of brown rice fermentation filtrate and 2.0 parts of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 1
The composition comprises the following components in parts by weight: 0 part of ergothioneine pine mushroom extract, 2.0 parts of brown rice fermentation filtrate and 1.0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 2
The composition consists of the following components in parts by weight: 1.0 part of ergothioneine pine mushroom extract, 0 part of brown rice fermentation filtrate and 1.0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 3
The blue-light resisting skin care composition containing the ergothioneine matsutake extract, the brown rice fermentation filtrate and the acetyl chitosamine comprises the following components in parts by weight: 1.0 part of ergothioneine pine mushroom extract, 2.0 parts of brown rice fermentation filtrate and 0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 4
The blue-light resisting skin care composition containing the ergothioneine matsutake extract, the brown rice fermentation filtrate and the acetyl chitosamine comprises the following components in parts by weight: 0 part of ergothioneine pine mushroom extract, 0 part of brown rice fermentation filtrate and 1.0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 5
The blue-light resisting skin care composition containing the ergothioneine matsutake extract, the brown rice fermentation filtrate and the acetyl chitosamine comprises the following components in parts by weight: 1.0 part of ergothioneine pine mushroom extract, 0 part of brown rice fermentation filtrate and 0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 6
The blue-light resisting skin care composition containing the ergothioneine matsutake extract, the brown rice fermentation filtrate and the acetyl chitosamine comprises the following components in parts by weight: 0 part of ergothioneine pine mushroom extract, 2.0 parts of brown rice fermentation filtrate and 0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 7
The blue-light resisting skin care composition containing the ergothioneine matsutake extract, the brown rice fermentation filtrate and the acetyl chitosamine comprises the following components in parts by weight: 1.0 part of ergothioneine pine mushroom extract, 2.0 parts of brown rice fermentation filtrate and 2.5 parts of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 8
The blue-light-resistant skin care composition containing the ergothioneine matsutake extract, the brown rice fermentation filtrate and the acetyl chitosamine comprises the following components in parts by weight: 3.0 parts of ergothioneine pine mushroom extract, 2.0 parts of brown rice fermentation filtrate and 1.0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 9
The blue-light-resistant skin care composition containing the ergothioneine matsutake extract, the brown rice fermentation filtrate and the acetyl chitosamine comprises the following components in parts by weight: 1.0 part of ergothioneine pine mushroom extract, 6.0 parts of brown rice fermentation filtrate and 1.0 part of acetyl chitosamine.
The preparation method is the same as example 1.
Comparative example 10
The blue light resisting skin care composition comprises the following components in parts by weight: 0.1 part of carnosine, 4.0 parts of brown rice fermentation filtrate and 0.2 part of acetyl chitosamine.
The preparation method is the same as example 1.
Application example
The compositions prepared in the above examples and comparative examples can be used in cosmetics. The types of cosmetics include, but are not limited to, lotions, essences, milks, creams, masks, introductions, color cosmetics, and the like. In addition to the above-mentioned composition, the cosmetic also contains the matrix necessary for making various dosage forms, and the matrix can be selected from the conventional matrix of various dosage forms in the prior art, and the preparation method of the cosmetic is that the cosmetic is prepared according to the conventional method in the prior art.
In order to verify the excellent effects of the composition containing ergothioneine matsutake extract, brown rice fermentation filtrate, and acetylcysteine according to the present invention, the compositions prepared in the above examples and comparative examples were prepared as anti-blue essence water. The content of the anti-blue essence water basic formula and each composition in the anti-blue essence water is shown in the following tables 1 and 2:
the preparation method comprises the following steps:
(1) Mixing and stirring the phase A raw materials uniformly;
(2) And adding the phase B and the phase C into the phase A, mixing and stirring uniformly, and discharging to obtain the composite material.
The blue light resisting essence prepared by the application example is adopted to verify the blue light resisting effect of the product.
Experimental example 1 antioxidant Activity
1.1 hydroxyl radical scavenging Rate experiments
The method comprises the following steps: taking 1.0mL of an absolute ethanol solution of 1.865mmol/L o-phenanthroline, adding 2mL of 0.2mol/L phosphate buffer solution with pH of 7.4 and 1mL of anti-blue light essence water respectively, fully and uniformly mixing, adding 1.0mL of 1.865mmol/L FeSO 4 ·7H 2 Mixing O solution, adding 1.0mL of 0.03% (v/v) H 2 O 2 Respectively measuring absorbance values of the mixed solutions at 536nm in constant temperature water bath at 37 deg.C for 60min to obtain A1, measuring absorbance values with distilled water instead of composition as blank group to obtain A2, and replacing H with distilled water 2 O 2 The absorbance value of the damage group is measured as A3, and the hydroxyl radical clearance of the sample is calculated according to the following formula: hydroxyl radical clearance (%) = [ (A1-A3)/(A2-A3)]X 100%, the average of three measurements was taken, and the results are shown in table 3.
Radical scavenging rate experiment
The method comprises the following steps: taking 3mL of DPPH-anhydrous methanol solution with the concentration of 0.0178 mmol/L, adding 3mL of blue-light resistant essence water into a cuvette, uniformly mixing, immediately measuring the light absorption value at the wavelength of 517nm, marking the light absorption value as A1, keeping the cuvette at 37 ℃ in the dark for 30min, and then measuring the light absorption value as A2, wherein the blank control is only adding DPPH-anhydrous methanol solution, and the light absorption value is marked as A3. DPPH radical clearance was calculated as follows: DPPH radical clearance (%) = [1- (A1-A2)/A3 ] × 100%, and the results of detection are shown in table 3, taking the average of three measurements.
As can be seen from Table 3, the ergothioneine matsutake extract, brown rice fermented filtrate and acetylcysteine-containing composition of the formulation of the present invention has more excellent hydroxyl radical scavenging rate and DPPH radical scavenging rate, and the ergothioneine matsutake extract, brown rice fermented filtrate and acetylcysteine have excellent synergistic effects as analyzed by comparing examples 1-7 with comparative examples 1-6; it can be obtained by analyzing example 1 and comparative examples 7 to 9 that the selected content of the present invention is excellent in effect, and the increase in content beyond this content range does not bring about a doubling of the effect. Carnosine is a multifunctional active peptide, can effectively quench active oxygen free radicals, can complex heavy metals, has other effects of detoxifying and detoxifying heavy metals and the like, and has a better effect in the anti-blue-light composition. It can be seen from the analysis of example 2 and comparative example 10 that the replacement of the ergothioneine matsutake extract in the composition with carnosine commonly used on the market, at the same content, did not differ significantly in the rate of hydroxyl radical scavenging and DPPH radical scavenging.
Experimental example 2 whitening Effect
Cell culture solution: 1640 culture medium containing 10% FBS; naOH lysate: preparing lysis solution with the concentration of 0.1 mol/L by using 10% DMSO solution; monkey head extract solution: preparing 20 mu M of a mao monkey hormone solution by using a cell culture solution; sample solution: a4% solution was prepared from a Macacan mulatta solution and examples 1 to 7 and comparative examples 1 to 6 and 10 as a sample solution; MTT: and (3) preparing an MTT (methyl thiazolyl tetrazolium) acting solution by using PBS (phosphate buffer solution), filtering and sterilizing by using a filter membrane of 0.22 mu m, and storing for later use in a refrigerator at 4 ℃.
B16 cells were plated at 1X 10 5 Inoculating in 6-well plate at a density of 5% CO at 37 deg.C 2 Culturing for 24h under the condition. Adding sample solution, reacting for 72 hr, cracking cell with NaOH lysate, heating at 80 deg.C for 30min, and detecting with microplate readerThe absorbance. Meanwhile, cells with the same volume density are inoculated in a 96-well plate to serve as proliferation control, and the influence of the sample on the melanin level in a unit cell is examined. The results are shown in Table 4.
From the data analysis of examples 1 to 7 and comparative examples 1 to 6, it can be seen that the ergothioneine matsutake extract, the brown rice fermented filtrate and the acetyl chitosamine have synergistic whitening effect, and from the comparison analysis of example 2 and comparative example 10, it can be seen that the composition of the present invention has superior whitening effect at the same content by replacing the ergothioneine matsutake extract in the composition with carnosine, which is a commonly used anti-blue light raw material in the market.
Experimental example 3 Effect of regulating biological rhythm
The main component of the B-Circadin raw material developed by Laien corporation of Switzerland is the extract of the leaf/stem of Lespedeza capitata, and the contained active components of echinacoside and isoschaftoside are directly involved in maintaining the biological rhythm. It was found that a concentration of 3% B-Circadin had the best results, so this test was conducted using 3% B-Circadin in comparison with example 1.
Volunteers: 18 women of 25-35 years old are healthy, live for 2 weeks according to the same work and rest rule and diet rule, and are subjected to experiments during which the work and rest rule and the diet rule are the same.
Sample preparation: blue light prevention essence prepared in example 1; example 1 was replaced with anti-blue essence water at a concentration of 3wt% -B-Circadin.
The test method comprises the following steps: the right face was smeared with blue-light-proof essence containing example 1, the left face was smeared with blue-light-proof essence containing 3% by weight of b-Circadin, then the volunteers were arranged to watch the artificial screen at night, the double-blind half-face test was performed, and the volunteers were allowed to perform self-evaluation 5 days after the experiment.
Evaluation items: skin fatigue symptoms, wherein the skin fatigue symptoms are comprehensively evaluated from the following aspects: dark and deep pores, oily or acne skin, dark eye circles, pouches, dry lines, poor skin absorption, and the like.
Evaluation criteria: the volunteers subjectively evaluate the change condition of the skin fatigue symptom according to the skin condition of the volunteers.
And (3) test results: of the 18 volunteers, 50% of the persons felt about the fatigue symptoms with almost the same effect, 22.2% of the persons felt about the fatigue symptoms with better effect than the left face, and 27.8% of the persons felt about the fatigue symptoms with better effect than the right face. It can be seen that the composition of the present invention comprising ergothioneine matsutake mushroom extract, brown rice fermentation filtrate and acetylcysteine has the effect of regulating biological rhythm.
As can be seen from the experimental data of experimental examples 1-3, the composition containing ergothioneine matsutake mushroom extract, brown rice fermentation filtrate and acetyl chitosamine of the present invention has excellent performance in improving pigmentation, delaying skin aging, and regulating biological rhythm, and can achieve more comprehensive blue light prevention effect through three aspects, and the combination of the three has excellent blue light prevention effect.
Claims (9)
1. A blue light resisting composition containing ergothioneine extract, brown rice fermentation filtrate and acetyl chitosamine is characterized by comprising the following effective components in parts by weight: 0.1-2.0 parts of ergothioneine extract, 0.1-4.0 parts of brown rice fermentation filtrate and 0.1-2.0 parts of acetyl chitosamine;
the ergothioneine extract is prepared from Hericium Erinaceus (Hericium Erinaceus)Hericium erinaceus ) CCTCC No: and (3) treating the fermentation liquor obtained by the fermentation of M2018567 to obtain an extracting solution, wherein the content of ergothioneine in the ergothioneine extracting solution is more than or equal to 200mg/L.
2. The blue light-resistant composition according to claim 1, which is characterized by comprising the following effective ingredients in parts by weight: 0.5-2.0 parts of ergothioneine extract, 0.5-4.0 parts of brown rice fermentation filtrate and 0.5-2.0 parts of acetyl chitosamine.
3. The blue-light resistant composition of claim 1, wherein: the content of the ergothioneine in the ergothioneine extracting solution is more than or equal to 300 mg/L.
4. The blue-light resistant composition of claim 1, wherein: the brown rice fermentation filtrate is obtained by performing impurity removal and sterilization treatment on fermentation liquor obtained by fermenting brown rice with yeast.
5. Use of the anti-blue light composition comprising ergothioneine extract, brown rice fermented filtrate and acetyl chitosamine according to any one of claims 1-4 in the preparation of cosmetics for regulating skin biorhythm, delaying skin aging or improving pigmentation.
6. Use of the anti-blue light composition comprising ergothioneine extract, brown rice fermented filtrate and acetyl chitosamine according to any one of claims 1-4 in anti-blue light cosmetics.
7. A blue light resistant cosmetic is characterized in that: the effective components comprise the ergothioneine-containing extract as claimed in any one of claims 1 to 4, brown rice fermentation filtrate and acetyl chitosamine.
8. The blue-light resistant cosmetic as set forth in claim 7, wherein: the content of ergothioneine extract in cosmetic is 0.1-2.0wt%, the content of brown rice fermented filtrate in cosmetic is 0.1-4.0 wt%, and the content of acetyl chitosamine in cosmetic is 0.1-2.0wt%.
9. The blue-light resistant cosmetic as set forth in claim 7, wherein: the cosmetic comprises lotion, essence, milk, cream, facial mask, introduction liquid or color makeup.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| IT202000011776A1 (en) * | 2020-05-20 | 2021-11-20 | Mister Bio Food Srl | FORMULATION OF A FERMENTED VEGETABLE BLEND BASED ON SPROUTED BROWN RICE |
| CN111557875B (en) * | 2020-06-02 | 2023-03-10 | 华熙生物科技股份有限公司 | Skin care composition for resisting light pollution and light injury and preparation method and application thereof |
| CN111803397B (en) * | 2020-07-07 | 2022-12-02 | 华熙生物科技股份有限公司 | Scalp care product and preparation method thereof |
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| CN116440051A (en) * | 2022-12-29 | 2023-07-18 | 华熙生物科技股份有限公司 | Composition with moisturizing effect and application of composition in cosmetics |
| CN115944548B (en) * | 2023-02-03 | 2024-06-21 | 山东福瑞达生物股份有限公司 | Oil control composition for improving light stability of ergothioneine and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101851623B1 (en) * | 2017-07-25 | 2018-06-04 | 주식회사 아미코스메틱 | A cosmetic composition comprising spirulina extract for blue light interception |
| CN108324633A (en) * | 2018-03-01 | 2018-07-27 | 华熙福瑞达生物医药有限公司 | A kind of preparation method and products thereof of fermentative brown rice stoste used for cosmetic |
| EP3466419A1 (en) * | 2017-10-05 | 2019-04-10 | Thorel, Jean-Noël | Use of trimethoxybenzyl acetylsinapate, advantageously in combination with at least one sunscreen, for protecting the skin |
| CN109820739A (en) * | 2018-11-27 | 2019-05-31 | 广州瑞誉化工科技有限公司 | A kind of skin composition and its preparation method and application of Plantain skin lightening |
| CN109939027A (en) * | 2019-03-01 | 2019-06-28 | 华熙生物科技股份有限公司 | A kind of method of cosmetics stoste of the Hericium erinaceus fermentation preparation containing erythrothioneine |
| CN110200875A (en) * | 2019-07-09 | 2019-09-06 | 中山卡丝生物科技有限公司 | A kind of antiallergic whitening mask and preparation method thereof |
| CN113855608A (en) * | 2021-09-29 | 2021-12-31 | 广州万邦实业发展有限公司 | Skin care composition for resisting light pollution and light injury and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200237626A1 (en) * | 2019-01-28 | 2020-07-30 | Jamrm, Llc | Anti-Aging Corrective and Protective Formulation and Methods |
-
2019
- 2019-12-26 CN CN201911362073.4A patent/CN110917061B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101851623B1 (en) * | 2017-07-25 | 2018-06-04 | 주식회사 아미코스메틱 | A cosmetic composition comprising spirulina extract for blue light interception |
| EP3466419A1 (en) * | 2017-10-05 | 2019-04-10 | Thorel, Jean-Noël | Use of trimethoxybenzyl acetylsinapate, advantageously in combination with at least one sunscreen, for protecting the skin |
| CN108324633A (en) * | 2018-03-01 | 2018-07-27 | 华熙福瑞达生物医药有限公司 | A kind of preparation method and products thereof of fermentative brown rice stoste used for cosmetic |
| CN109820739A (en) * | 2018-11-27 | 2019-05-31 | 广州瑞誉化工科技有限公司 | A kind of skin composition and its preparation method and application of Plantain skin lightening |
| CN109939027A (en) * | 2019-03-01 | 2019-06-28 | 华熙生物科技股份有限公司 | A kind of method of cosmetics stoste of the Hericium erinaceus fermentation preparation containing erythrothioneine |
| CN110200875A (en) * | 2019-07-09 | 2019-09-06 | 中山卡丝生物科技有限公司 | A kind of antiallergic whitening mask and preparation method thereof |
| CN113855608A (en) * | 2021-09-29 | 2021-12-31 | 广州万邦实业发展有限公司 | Skin care composition for resisting light pollution and light injury and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 《蓝光危害及抗蓝光技术在化妆品中的应用概况》;许锐林等;《香料香精化妆品》;20190630;第80-84页 * |
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