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CN118415341B - Composition for increasing bone mineral density and preparation method thereof - Google Patents

Composition for increasing bone mineral density and preparation method thereof Download PDF

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Publication number
CN118415341B
CN118415341B CN202410840600.2A CN202410840600A CN118415341B CN 118415341 B CN118415341 B CN 118415341B CN 202410840600 A CN202410840600 A CN 202410840600A CN 118415341 B CN118415341 B CN 118415341B
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parts
powder
extract
eucommia ulmoides
composition
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CN118415341A (en
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陈忠平
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Lancy Purcell Biotechnology Guangzhou Co ltd
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Lancy Purcell Biotechnology Guangzhou Co ltd
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Abstract

The invention belongs to the technical field of biological medicines, and particularly discloses a composition for increasing bone mineral density and a preparation method thereof, wherein the composition comprises the following components: the composition disclosed by the invention can effectively maintain blood calcium balance, supplement calcium of a human body and reduce loss of calcium ions, can excite and promote growth of bone cells, can supplement collagen in bones of the human body, effectively promote synthesis of new cartilage tissues and proteoglycan, improve generation of hyaluronic acid in joints, effectively improve bone density, improve toughness and hardness of bones, effectively improve and prevent problems of osteoporosis, osteoarticular discomfort and the like.

Description

Composition for increasing bone mineral density and preparation method thereof
Technical Field
The invention relates to the technical field of biological medicines, in particular to a composition for increasing bone mineral density and a preparation method thereof.
Background
With the gradual increase of the aging degree of people, osteoporosis gradually becomes a key factor of healthy life of people. In addition, fracture is one of the important factors of bone injury, and fracture refers to a complete or partial fracture of bone or bone structure, which is often found in children and the elderly, and sometimes occurs in young and middle-aged people. Bone diseases such as osteoporosis are becoming an important factor affecting the quality of life of people. Bone mineral density is called bone mineral density, and bone mineral density is an important index for measuring bone health, and improving bone mineral density has important significance for preventing bone diseases such as osteoporosis.
At present, although some products for increasing bone mineral density exist on the market, the effect is not remarkable, and the application is proposed in view of the fact.
Disclosure of Invention
The invention provides a composition for increasing bone mineral density and a preparation method thereof.
The invention solves the technical problems by adopting the following technical scheme:
A composition for increasing bone mineral density, comprising the following components in parts by weight: 8-15 parts of hydrolyzed collagen, 15-22 parts of calcium lactate, 6-10 parts of D-glucosamine potassium sulfate, 4-8 parts of casein phosphopeptide, 8-12 parts of cyclodextrin, 0.1-0.4 part of citric acid, 0.5-1 part of stevioside, 16-22 parts of dogwood extract and 20-25 parts of eucommia ulmoides extract.
The invention creatively combines the raw materials according to specific parts by weight to obtain the composition capable of effectively increasing bone density, effectively maintaining blood calcium balance, supplementing calcium of human body and reducing loss of calcium ions, exciting and promoting growth of bone cells, supplementing collagen in bones of human body, effectively promoting synthesis of new cartilage tissues and proteoglycan, improving generation of hyaluronic acid in joints, effectively improving bone density, improving toughness and hardness of bones, effectively improving and preventing osteoporosis, bone joint discomfort and other problems, and has wide application prospect.
As a preferred embodiment of the present invention, the composition comprises the following components in parts by weight: 12 parts of hydrolyzed collagen, 20 parts of calcium lactate, 8 parts of D-glucosamine potassium sulfate, 5 parts of casein phosphopeptide, 10 parts of cyclodextrin, 0.2 part of citric acid, 0.8 part of stevioside, 20 parts of dogwood extract and 24 parts of eucommia ulmoides extract.
As a preferred embodiment of the invention, the preparation method of the dogwood extract comprises the following steps:
(1) Pulverizing dogwood to 50-200 meshes to obtain dogwood powder;
(2) Crushing calcined oyster shell to 50-200 meshes to obtain calcined oyster shell powder;
(3) Adding cellulase, keratinase, alkaline proteinase, cornus officinalis powder, calcined oyster powder and milk mineral salt into water, and performing enzymolysis to obtain an enzymolysis solution;
(4) And (3) injecting the enzymolysis liquid into a carbon dioxide fluid reaction kettle for supercritical treatment, and drying to obtain the dogwood extract.
The invention creatively carries out enzymolysis on the cornel powder, the calcined oyster powder and the milk mineral salt, wherein the calcined oyster powder and the milk mineral salt can effectively improve the stability of the effective components of the cornel, can supplement calcium of human bodies and reduce the loss of calcium ions, can excite and promote the growth of bone cells in the preparation process, and can improve the solubility of polysaccharide and amino acid in the calcined oyster in the enzymolysis process of volatile oil and organic acid in the cornel, so as to improve the extraction rate of the polysaccharide and amino acid, and further promote the synthesis of new cartilage tissues and proteoglycan, thereby effectively improving the bone density.
As a preferred embodiment of the invention, the mass ratio of the cellulase, the keratinase, the alkaline protease, the dogwood powder, the calcined oyster powder, the milk mineral salt and the water is (0.005-0.02): (0.005-0.02): (0.005-0.02): 1: (0.1 to 0.4): (0.05-0.1): (4-10).
As a preferred embodiment of the invention, the enzymolysis temperature is 45-52 ℃ and the enzymolysis time is 2-6 hours.
As a preferred embodiment of the present invention, the conditions of the supercritical treatment are: the temperature is 30-40 ℃ and the pressure is 6-10 MPa the flow rate of CO 2 is 0.4-0.8L/min, and the time is 20-50 min.
As a preferred embodiment of the invention, the preparation method of the eucommia ulmoides extract comprises the following steps:
(1) Crushing eucommia ulmoides to 50-200 meshes to obtain eucommia ulmoides powder;
(2) Adding eucommia ulmoides powder into deionized water, and performing ultrasonic treatment to obtain a mixed solution, wherein the feed liquid ratio of the eucommia ulmoides powder to the deionized water is 1g: 4-10 ml;
(3) Adding the mixed solution into absolute ethyl alcohol, carrying out microwave treatment and centrifugation to obtain supernatant, wherein the volume ratio of the mixed solution to the absolute ethyl alcohol is 1: 2-5;
(4) And regulating the pH of the supernatant to 6.2-6.8, performing silica gel column chromatography, eluting with 5-15wt% of methanol aqueous solution, eluting with 30-50wt% of methanol aqueous solution, collecting 30-50wt% of methanol aqueous solution eluent, and drying to obtain the eucommia ulmoides extract.
As a preferred embodiment of the invention, the power of the ultrasonic treatment is 300-600W, and the time of the ultrasonic treatment is 20-40 min; and/or
The microwave treatment power is 200-600W, and the microwave treatment time is 6-15 min.
The invention also provides a preparation method of the composition for increasing bone mineral density, which comprises the following steps:
Mixing hydrolyzed collagen, calcium lactate, D-glucosamine potassium sulfate, casein phosphopeptide, cyclodextrin, citric acid, stevioside, corni fructus extract, and Eucommiae cortex extract to obtain composition for increasing bone density.
The invention has the beneficial effects that: the composition can effectively maintain the balance of blood and calcium, supplement calcium of a human body, reduce the loss of calcium ions, excite and promote the growth of bone cells, supplement collagen in bones of the human body, effectively promote the synthesis of new cartilage tissues and proteoglycan, improve the generation of hyaluronic acid in joints, effectively improve the bone density, improve the toughness and the hardness of bones, effectively improve and prevent the problems of osteoporosis, bone joint discomfort and the like, and has wide application prospect.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the invention, the technical characteristics described in an open mode comprise a closed technical scheme composed of the listed characteristics and also comprise an open technical scheme comprising the listed characteristics.
In the present invention, the numerical ranges are referred to as continuous, and include the minimum and maximum values of the ranges, and each value between the minimum and maximum values, unless otherwise specified. Further, when a range refers to an integer, each integer between the minimum and maximum values of the range is included. Further, when multiple range description features or characteristics are provided, the ranges may be combined. In other words, unless otherwise indicated, all ranges disclosed herein are to be understood to include any and all subranges subsumed therein.
In the present invention, the specific dispersing and stirring treatment method is not particularly limited.
The reagents or instruments used in the invention are not pointed out by manufacturers, are all conventional products which can be obtained commercially, and the raw materials used in each comparative example and the raw materials used in the experiment parallel to each example are all the same commercial products except for specific descriptions.
Example 1
A composition for increasing bone mineral density, comprising the following components in parts by weight: 12 parts of hydrolyzed collagen, 20 parts of calcium lactate, 8 parts of D-glucosamine potassium sulfate, 5 parts of casein phosphopeptide, 10 parts of cyclodextrin, 0.2 part of citric acid, 0.8 part of stevioside, 20 parts of dogwood extract and 24 parts of eucommia ulmoides extract.
The preparation method of the dogwood extract comprises the following steps:
(1) Pulverizing Corni fructus to 100 mesh to obtain Corni fructus powder;
(2) Pulverizing Concha Ostreae Preparata to 100 mesh to obtain Concha Ostreae Preparata powder;
(3) Adding cellulase, keratinase, alkaline proteinase, cornus officinalis powder, calcined oyster powder and milk mineral salt into water, and performing enzymolysis for 5 hours at 50 ℃ to obtain enzymolysis liquid; the mass ratio of the cellulase to the keratinase to the alkaline protease to the dogwood powder to the calcined oyster powder to the milk mineral salt to the water is 0.01:0.01:0.01:1:0.2:0.08:8, 8;
(4) Injecting the enzymolysis liquid into a carbon dioxide fluid reaction kettle for supercritical treatment, and drying to obtain the dogwood extract, wherein the supercritical treatment conditions are as follows: the temperature is 35 ℃, the pressure is 8MPa, the flow rate of CO 2 is 0.5L/min, and the time is 30min.
The preparation method of the eucommia ulmoides extract comprises the following steps:
(1) Crushing eucommia ulmoides to 100 meshes to obtain eucommia ulmoides powder;
(2) Adding eucommia ulmoides powder into deionized water, and performing ultrasonic treatment at 500W for 25min to obtain a mixed solution, wherein the feed liquid ratio of the eucommia ulmoides powder to the deionized water is 1g:6ml;
(3) Adding the mixed solution into absolute ethyl alcohol, treating for 25min by using 500W microwaves, and centrifuging for 10min at a rotation speed of 5000rpm to obtain supernatant, wherein the volume ratio of the mixed solution to the absolute ethyl alcohol is 1:4, a step of;
(4) Regulating pH of the supernatant to 6.5, performing silica gel column chromatography, eluting with 10wt% methanol water solution, eluting with 40wt% methanol water solution, collecting 40wt% methanol water solution eluate, and drying to obtain Eucommiae cortex extract.
The preparation method of the composition for increasing bone mineral density comprises the following steps:
Mixing hydrolyzed collagen, calcium lactate, D-glucosamine potassium sulfate, casein phosphopeptide, cyclodextrin, citric acid, stevioside, corni fructus extract, and Eucommiae cortex extract to obtain composition for increasing bone density.
Example 2
A composition for increasing bone mineral density, comprising the following components in parts by weight: 8 parts of hydrolyzed collagen, 22 parts of calcium lactate, 6 parts of D-glucosamine potassium sulfate, 8 parts of casein phosphopeptide, 12 parts of cyclodextrin, 0.1 part of citric acid, 1 part of stevioside, 16 parts of dogwood extract and 25 parts of eucommia ulmoides extract.
The preparation method of the dogwood extract comprises the following steps:
(1) Pulverizing Corni fructus to 100 mesh to obtain Corni fructus powder;
(2) Pulverizing Concha Ostreae Preparata to 100 mesh to obtain Concha Ostreae Preparata powder;
(3) Adding cellulase, keratinase, alkaline proteinase, cornus officinalis powder, calcined oyster powder and milk mineral salt into water, and performing enzymolysis for 5 hours at 50 ℃ to obtain enzymolysis liquid; the mass ratio of the cellulase to the keratinase to the alkaline protease to the dogwood powder to the calcined oyster powder to the milk mineral salt to the water is 0.01:0.01:0.01:1:0.2:0.08:8, 8;
(4) Injecting the enzymolysis liquid into a carbon dioxide fluid reaction kettle for supercritical treatment, and drying to obtain the dogwood extract, wherein the supercritical treatment conditions are as follows: the temperature is 35 ℃, the pressure is 8MPa, the flow rate of CO 2 is 0.5L/min, and the time is 30min.
The preparation method of the eucommia ulmoides extract comprises the following steps:
(1) Crushing eucommia ulmoides to 100 meshes to obtain eucommia ulmoides powder;
(2) Adding eucommia ulmoides powder into deionized water, and performing ultrasonic treatment at 500W for 25min to obtain a mixed solution, wherein the feed liquid ratio of the eucommia ulmoides powder to the deionized water is 1g:6ml;
(3) Adding the mixed solution into absolute ethyl alcohol, treating for 25min by using 500W microwaves, and centrifuging for 10min at a rotation speed of 5000rpm to obtain supernatant, wherein the volume ratio of the mixed solution to the absolute ethyl alcohol is 1:4, a step of;
(4) Regulating pH of the supernatant to 6.5, performing silica gel column chromatography, eluting with 10wt% methanol water solution, eluting with 40wt% methanol water solution, collecting 40wt% methanol water solution eluate, and drying to obtain Eucommiae cortex extract.
The preparation method of the composition for increasing bone mineral density comprises the following steps:
Mixing hydrolyzed collagen, calcium lactate, D-glucosamine potassium sulfate, casein phosphopeptide, cyclodextrin, citric acid, stevioside, corni fructus extract, and Eucommiae cortex extract to obtain composition for increasing bone density.
Example 3
A composition for increasing bone mineral density, comprising the following components in parts by weight: 15 parts of hydrolyzed collagen, 15 parts of calcium lactate, 10 parts of D-glucosamine potassium sulfate, 4 parts of casein phosphopeptide, 8 parts of cyclodextrin, 0.4 part of citric acid, 0.5 part of stevioside, 22 parts of dogwood extract and 20 parts of eucommia ulmoides extract.
The preparation method of the dogwood extract comprises the following steps:
(1) Pulverizing Corni fructus to 100 mesh to obtain Corni fructus powder;
(2) Pulverizing Concha Ostreae Preparata to 100 mesh to obtain Concha Ostreae Preparata powder;
(3) Adding cellulase, keratinase, alkaline proteinase, cornus officinalis powder, calcined oyster powder and milk mineral salt into water, and performing enzymolysis for 5 hours at 50 ℃ to obtain enzymolysis liquid; the mass ratio of the cellulase to the keratinase to the alkaline protease to the dogwood powder to the calcined oyster powder to the milk mineral salt to the water is 0.01:0.01:0.01:1:0.2:0.08:8, 8;
(4) Injecting the enzymolysis liquid into a carbon dioxide fluid reaction kettle for supercritical treatment, and drying to obtain the dogwood extract, wherein the supercritical treatment conditions are as follows: the temperature is 35 ℃, the pressure is 8MPa, the flow rate of CO 2 is 0.5L/min, and the time is 30min.
The preparation method of the eucommia ulmoides extract comprises the following steps:
(1) Crushing eucommia ulmoides to 100 meshes to obtain eucommia ulmoides powder;
(2) Adding eucommia ulmoides powder into deionized water, and performing ultrasonic treatment at 500W for 25min to obtain a mixed solution, wherein the feed liquid ratio of the eucommia ulmoides powder to the deionized water is 1g:6ml;
(3) Adding the mixed solution into absolute ethyl alcohol, treating for 25min by using 500W microwaves, and centrifuging for 10min at a rotation speed of 5000rpm to obtain supernatant, wherein the volume ratio of the mixed solution to the absolute ethyl alcohol is 1:4, a step of;
(4) Regulating pH of the supernatant to 6.5, performing silica gel column chromatography, eluting with 10wt% methanol water solution, eluting with 40wt% methanol water solution, collecting 40wt% methanol water solution eluate, and drying to obtain Eucommiae cortex extract.
The preparation method of the composition for increasing bone mineral density comprises the following steps:
Mixing hydrolyzed collagen, calcium lactate, D-glucosamine potassium sulfate, casein phosphopeptide, cyclodextrin, citric acid, stevioside, corni fructus extract, and Eucommiae cortex extract to obtain composition for increasing bone density.
Example 4
A composition for increasing bone mineral density, comprising the following components in parts by weight: 10 parts of hydrolyzed collagen, 18 parts of calcium lactate, 9 parts of D-glucosamine potassium sulfate, 7 parts of casein phosphopeptide, 9 parts of cyclodextrin, 0.3 part of citric acid, 0.7 part of stevioside, 21 parts of dogwood extract and 23 parts of eucommia ulmoides extract.
The preparation method of the dogwood extract comprises the following steps:
(1) Pulverizing Corni fructus to 100 mesh to obtain Corni fructus powder;
(2) Pulverizing Concha Ostreae Preparata to 100 mesh to obtain Concha Ostreae Preparata powder;
(3) Adding cellulase, keratinase, alkaline proteinase, cornus officinalis powder, calcined oyster powder and milk mineral salt into water, and performing enzymolysis for 5 hours at 50 ℃ to obtain enzymolysis liquid; the mass ratio of the cellulase to the keratinase to the alkaline protease to the dogwood powder to the calcined oyster powder to the milk mineral salt to the water is 0.01:0.01:0.01:1:0.2:0.08:8, 8;
(4) Injecting the enzymolysis liquid into a carbon dioxide fluid reaction kettle for supercritical treatment, and drying to obtain the dogwood extract, wherein the supercritical treatment conditions are as follows: the temperature is 35 ℃, the pressure is 8MPa, the flow rate of CO 2 is 0.5L/min, and the time is 30min.
The preparation method of the eucommia ulmoides extract comprises the following steps:
(1) Crushing eucommia ulmoides to 100 meshes to obtain eucommia ulmoides powder;
(2) Adding eucommia ulmoides powder into deionized water, and performing ultrasonic treatment at 500W for 25min to obtain a mixed solution, wherein the feed liquid ratio of the eucommia ulmoides powder to the deionized water is 1g:6ml;
(3) Adding the mixed solution into absolute ethyl alcohol, treating for 25min by using 500W microwaves, and centrifuging for 10min at a rotation speed of 5000rpm to obtain supernatant, wherein the volume ratio of the mixed solution to the absolute ethyl alcohol is 1:4, a step of;
(4) Regulating pH of the supernatant to 6.5, performing silica gel column chromatography, eluting with 10wt% methanol water solution, eluting with 40wt% methanol water solution, collecting 40wt% methanol water solution eluate, and drying to obtain Eucommiae cortex extract.
The preparation method of the composition for increasing bone mineral density comprises the following steps:
Mixing hydrolyzed collagen, calcium lactate, D-glucosamine potassium sulfate, casein phosphopeptide, cyclodextrin, citric acid, stevioside, corni fructus extract, and Eucommiae cortex extract to obtain composition for increasing bone density.
Comparative example 1
Comparative example 1 is different from example 1 in that comparative example 1 does not contain a cornel extract, and an equivalent amount of eucommia ulmoides extract is used instead of cornel extract, all of which are the same.
A composition for increasing bone mineral density, comprising the following components in parts by weight: 12 parts of hydrolyzed collagen, 20 parts of calcium lactate, 8 parts of D-glucosamine potassium sulfate, 5 parts of casein phosphopeptide, 10 parts of cyclodextrin, 0.2 part of citric acid, 0.8 part of stevioside and 44 parts of eucommia ulmoides extract.
Comparative example 2
Comparative example 2 is different from example 1 in that comparative example 2 does not contain eucommia ulmoides extract, and the eucommia ulmoides extract is replaced with the cornel extract in the same amount, and the other are the same.
A composition for increasing bone mineral density, comprising the following components in parts by weight: 12 parts of hydrolyzed collagen, 20 parts of calcium lactate, 8 parts of D-glucosamine potassium sulfate, 5 parts of casein phosphopeptide, 10 parts of cyclodextrin, 0.2 part of citric acid, 0.8 part of stevioside and 44 parts of dogwood extract.
Comparative example 3
Comparative example 3 is different from example 1 in that the preparation method of the cornel extract described in comparative example 3 is different and the other are the same.
The preparation method of the dogwood extract comprises the following steps:
(1) Pulverizing Corni fructus to 100 mesh to obtain Corni fructus powder;
(2) Adding cellulase, keratinase, alkaline proteinase, cornus officinalis powder and milk mineral salt into water, and performing enzymolysis for 5 hours at 50 ℃ to obtain enzymolysis liquid; the mass ratio of the cellulase to the keratinase to the alkaline protease to the dogwood powder to the milk mineral salt to the water is 0.01:0.01:0.01:1:0.2:0.08:8, 8;
(3) Injecting the enzymolysis liquid into a carbon dioxide fluid reaction kettle for supercritical treatment, and drying to obtain the dogwood extract, wherein the supercritical treatment conditions are as follows: the temperature is 35 ℃, the pressure is 8MPa, the flow rate of CO 2 is 0.5L/min, and the time is 30min.
Comparative example 4
Comparative example 4 is different from example 1 in that the preparation method of the cornus officinalis extract described in comparative example 4 is different, and the other are the same.
The preparation method of the dogwood extract comprises the following steps:
(1) Pulverizing Corni fructus to 100 mesh to obtain Corni fructus powder;
(2) Pulverizing Concha Ostreae Preparata to 100 mesh to obtain Concha Ostreae Preparata powder;
(3) Adding cellulase, keratinase, alkaline proteinase, cornus officinalis powder, calcined oyster powder and milk mineral salt into water, and performing enzymolysis for 5 hours at 50 ℃ to obtain enzymolysis liquid; the mass ratio of the cellulase to the keratinase to the alkaline protease to the dogwood powder to the calcined oyster powder to the milk mineral salt to the water is 0.01:0.01:0.01:1:0.2:0.08:8, 8;
(4) And (5) drying the enzymolysis liquid to obtain the dogwood extract.
Comparative example 5
Comparative example 5 is different from example 1 in that the preparation method of eucommia ulmoides extract described in comparative example 5 is different from example 1, and the other are the same.
The preparation method of the eucommia ulmoides extract comprises the following steps:
(1) Crushing eucommia ulmoides to 100 meshes to obtain eucommia ulmoides powder;
(2) Adding eucommia ulmoides powder into deionized water, and performing ultrasonic treatment at 500W for 25min to obtain a mixed solution, wherein the feed liquid ratio of the eucommia ulmoides powder to the deionized water is 1g:6ml; drying to obtain eucommia ulmoides extract.
Test case
And randomly dividing 144 weight of 75-85 g weaned SPF-class female rats into 12 groups of 12 rats each. The blank control group, the model control group, the positive control group and the test group are respectively compositions of examples and comparative examples. In addition to the placebo group, the other groups were fed low calcium diet continuously to prepare low bone mass model rats, while each group was fed the corresponding test article. The positive control group was fed with 150mg/kg of calcium carbonate, the test group was fed with 150mg/kg of the corresponding composition, the model control group was given a corresponding volume of distilled water, and the normal control group was fed with the normal feed and a corresponding volume of distilled water, each for 10 weeks.
The formula of the low-calcium feed comprises the following components: methionine 0.4%, choline chloride 0.4%, soybean powder 12%, casein 11%, soybean oil 5%, flour 50%, cellulose 1.5%, vitamin 1%, calcium-free mineral salt 2.6%, and starch in balance.
Determination of bone mineral density: the lumbar bone density of the rat was measured 1h after the end of the test using a dual-energy X-ray bone densitometer.
Table 1 bone mineral density test results table
Annotation: P <0.05 for comparison to the placebo group; # is P <0.05 compared to the model control group.
As can be seen from table 1, the composition of the present invention is effective in increasing bone density.
As can be seen from comparative examples 1 to 4, example 1 is the best mode for carrying out the present invention, and has the best effect of improving bone mineral density.
As can be seen from comparative examples 1 and 1-2, the eucommia ulmoides extract and the dogwood extract have remarkable synergistic effect in improving bone mineral density.
As can be seen from comparative examples 1 and 3-4, the cornel extracts prepared by different methods are different in improving bone density, and the cornel extracts prepared by the method provided by the invention can be used for remarkably improving bone density.
Finally, it should be noted that the above-mentioned embodiments illustrate rather than limit the scope of the invention, and that those skilled in the art will understand that changes can be made to the technical solutions of the invention or equivalents thereof without departing from the spirit and scope of the technical solutions of the invention.

Claims (6)

1. A composition for increasing bone mineral density, comprising the following components in parts by weight: 8-15 parts of hydrolyzed collagen, 15-22 parts of calcium lactate, 6-10 parts of D-glucosamine potassium sulfate, 4-8 parts of casein phosphopeptide, 8-12 parts of cyclodextrin, 0.1-0.4 part of citric acid, 0.5-1 part of stevioside, 16-22 parts of dogwood extract and 20-25 parts of eucommia ulmoides extract;
The preparation method of the dogwood extract comprises the following steps:
(1) Pulverizing dogwood to 50-200 meshes to obtain dogwood powder;
(2) Crushing calcined oyster shell to 50-200 meshes to obtain calcined oyster shell powder;
(3) Adding cellulase, keratinase, alkaline proteinase, cornus officinalis powder, calcined oyster powder and milk mineral salt into water, and performing enzymolysis to obtain an enzymolysis solution;
(4) Injecting the enzymolysis liquid into a carbon dioxide fluid reaction kettle for supercritical treatment, and drying to obtain a dogwood extract;
The mass ratio of the cellulase to the keratinase to the alkaline protease to the cornus officinalis powder to the calcined oyster shell powder to the milk mineral salt to the water is (0.005-0.02): (0.005-0.02): (0.005-0.02): 1: (0.1 to 0.4): (0.05-0.1): (4-10);
the preparation method of the eucommia ulmoides extract comprises the following steps:
(1) Crushing eucommia ulmoides to 50-200 meshes to obtain eucommia ulmoides powder;
(2) Adding eucommia ulmoides powder into deionized water, and performing ultrasonic treatment to obtain a mixed solution, wherein the feed liquid ratio of the eucommia ulmoides powder to the deionized water is 1g: 4-10 ml;
(3) Adding the mixed solution into absolute ethyl alcohol, carrying out microwave treatment and centrifugation to obtain supernatant, wherein the volume ratio of the mixed solution to the absolute ethyl alcohol is 1: 2-5;
(4) And regulating the pH of the supernatant to 6.2-6.8, performing silica gel column chromatography, eluting with 5-15wt% of methanol aqueous solution, eluting with 30-50wt% of methanol aqueous solution, collecting 30-50wt% of methanol aqueous solution eluent, and drying to obtain the eucommia ulmoides extract.
2. The bone mineral density increasing composition of claim 1, comprising the following components in parts by weight: 12 parts of hydrolyzed collagen, 20 parts of calcium lactate, 8 parts of D-glucosamine potassium sulfate, 5 parts of casein phosphopeptide, 10 parts of cyclodextrin, 0.2 part of citric acid, 0.8 part of stevioside, 20 parts of dogwood extract and 24 parts of eucommia ulmoides extract.
3. The bone mineral density increasing composition of claim 1, wherein the temperature of the enzymatic hydrolysis is 45-52 ℃ and the time of the enzymatic hydrolysis is 2-6 hours.
4. The bone mineral density increasing composition of claim 1 wherein the conditions of the supercritical treatment are: the temperature is 30-40 ℃ and the pressure is 6-10 MPa the flow rate of CO 2 is 0.4-0.8L/min, and the time is 20-50 min.
5. The bone mineral density increasing composition of claim 1, wherein the power of the ultrasonic treatment is 300-600 w, and the time of the ultrasonic treatment is 20-40 min; and/or
The microwave treatment power is 200-600W, and the microwave treatment time is 6-15 min.
6. The method for preparing the bone mineral density increasing composition according to any one of claims 1 to 5, comprising the steps of:
Mixing hydrolyzed collagen, calcium lactate, D-glucosamine potassium sulfate, casein phosphopeptide, cyclodextrin, citric acid, stevioside, corni fructus extract, and Eucommiae cortex extract to obtain composition for increasing bone density.
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