CN118141106A - Plant sterol nano preparation and preparation method and application thereof - Google Patents
Plant sterol nano preparation and preparation method and application thereof Download PDFInfo
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- CN118141106A CN118141106A CN202410266662.7A CN202410266662A CN118141106A CN 118141106 A CN118141106 A CN 118141106A CN 202410266662 A CN202410266662 A CN 202410266662A CN 118141106 A CN118141106 A CN 118141106A
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- phytosterol
- plant sterol
- maltodextrin
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- 238000002360 preparation method Methods 0.000 title claims abstract description 52
- 235000002378 plant sterols Nutrition 0.000 title claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 57
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 28
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 28
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 28
- 238000003756 stirring Methods 0.000 claims abstract description 25
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 20
- 239000008367 deionised water Substances 0.000 claims abstract description 18
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 18
- 239000012074 organic phase Substances 0.000 claims abstract description 17
- 238000002390 rotary evaporation Methods 0.000 claims abstract description 16
- 239000000839 emulsion Substances 0.000 claims abstract description 14
- 238000004108 freeze drying Methods 0.000 claims abstract description 11
- 239000008346 aqueous phase Substances 0.000 claims abstract description 10
- 238000000265 homogenisation Methods 0.000 claims abstract description 9
- 239000012071 phase Substances 0.000 claims abstract description 7
- 229940079593 drug Drugs 0.000 claims abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 6
- 108010026206 Conalbumin Proteins 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 14
- 229920000136 polysorbate Polymers 0.000 claims description 13
- 102000004169 proteins and genes Human genes 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- LWZFANDGMFTDAV-BURFUSLBSA-N [(2r)-2-[(2r,3r,4s)-3,4-dihydroxyoxolan-2-yl]-2-hydroxyethyl] dodecanoate Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O LWZFANDGMFTDAV-BURFUSLBSA-N 0.000 claims description 6
- 239000004359 castor oil Substances 0.000 claims description 6
- 235000019438 castor oil Nutrition 0.000 claims description 6
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 6
- 235000011067 sorbitan monolaureate Nutrition 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000013376 functional food Nutrition 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 235000013305 food Nutrition 0.000 abstract description 6
- 235000019441 ethanol Nutrition 0.000 description 15
- 102000004338 Transferrin Human genes 0.000 description 11
- 108090000901 Transferrin Proteins 0.000 description 11
- 239000012581 transferrin Substances 0.000 description 11
- 235000018102 proteins Nutrition 0.000 description 8
- 239000002105 nanoparticle Substances 0.000 description 6
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241000282414 Homo sapiens Species 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 238000004821 distillation Methods 0.000 description 3
- 230000001804 emulsifying effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RGSFGYAAUTVSQA-UHFFFAOYSA-N Cyclopentane Chemical compound C1CCCC1 RGSFGYAAUTVSQA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 229940109262 curcumin Drugs 0.000 description 2
- 235000012754 curcumin Nutrition 0.000 description 2
- 239000004148 curcumin Substances 0.000 description 2
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229940071440 soy protein isolate Drugs 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- GNMCGMFNBARSIY-UHFFFAOYSA-N 1,2,3,4,4a,4b,5,6,7,8,8a,9,10,10a-tetradecahydrophenanthrene Chemical compound C1CCCC2C3CCCCC3CCC21 GNMCGMFNBARSIY-UHFFFAOYSA-N 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000011632 Caseins Human genes 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- -1 alcohol compound Chemical class 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- DMEGYFMYUHOHGS-UHFFFAOYSA-N heptamethylene Natural products C1CCCCCC1 DMEGYFMYUHOHGS-UHFFFAOYSA-N 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229940068065 phytosterols Drugs 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229940080237 sodium caseinate Drugs 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000021119 whey protein Nutrition 0.000 description 1
Classifications
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- A23L29/03—Organic compounds
- A23L29/045—Organic compounds containing nitrogen as heteroatom
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- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention provides a phytosterol nano-preparation and a preparation method and application thereof, belonging to the technical field of nano-preparations. The preparation method of the invention comprises the following steps: (1) Dissolving maltodextrin and ovotransferrin in deionized water to obtain a water phase; (2) Adding the phytosterol and the emulsifying agent into ethanol, and stirring until the phytosterol and the emulsifying agent are completely dissolved to obtain an organic phase; (3) Mixing the aqueous phase with the organic phase, and then sequentially carrying out homogenization and ultrasonic treatment to obtain mixed emulsion; (4) And removing ethanol in the mixed emulsion through rotary evaporation, and then performing freeze drying to obtain the plant sterol nano preparation. The nano phytosterol preparation prepared by the invention has high embedding rate, good re-solubility and dispersibility and high bioavailability, and can well improve the application of the phytosterol in foods and medicines.
Description
Technical Field
The invention relates to the technical field of nano preparations, in particular to a phytosterol nano preparation and a preparation method and application thereof.
Background
The phytosterol is an alcohol compound taking cyclopentane perhydro-phenanthrene as a framework, is white crystal powder or particles at normal temperature, is odorless and tasteless, has a melting point of 130-170 ℃, is insoluble in water, strong acid and strong alkali, and is slightly soluble in organic solvents such as acetone, absolute ethyl alcohol, diethyl ether, benzene, chloroform, petroleum ether and the like at normal temperature. Phytosterols are present in almost all vegetable foods, such as grains, legumes, vegetables, fruits and nuts, in amounts that vary significantly from plant to plant, with the greatest amount being found in oil crops. At present, the phytosterol has been proved to have various physiological functions of cholesterol reduction, cardiovascular disease reduction, cancer resistance, inflammation resistance, oxidation resistance, atherosclerosis resistance and the like, but the bioavailability of the phytosterol in a human body is extremely low due to the characteristics of being insoluble in water and slightly soluble in an organic solvent, so that the application of the phytosterol in foods or medicines is limited.
In order to solve the problem of poor water solubility of the phytosterol, the phytosterol can meet daily requirements of human beings, and at present, a nanoparticle technology is generally adopted to improve the water solubility of the phytosterol. The nanoparticle technology for embedding the bioactive substances has the advantages of site-directed targeting delivery and efficient cell absorption, and can also improve the solubility, stability and bioavailability of the embedded bioactive substances. For example, patent CN105410934a discloses a water-soluble protein-phytosterol nanoparticle and preparation and application thereof, and the phytosterol nanoparticle is prepared by embedding a water-soluble protein such as sodium caseinate, soy isolate protein or whey protein concentrate and freeze-drying the embedded phytosterol, although the patent improves the water solubility of the phytosterol to a certain extent, the particle size of the phytosterol nanoparticle prepared by the method is larger, and the water solubility, bioavailability, embedding rate, dispersibility, re-solubility and the like of the phytosterol nanoparticle are all required to be further improved.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a phytosterol nano-preparation, a preparation method and application thereof, and the prepared phytosterol nano-preparation has high embedding rate, good resolubility and dispersibility and high bioavailability, and can well improve the application of phytosterol in foods and medicines.
In order to achieve the technical effects, the invention adopts the following technical scheme:
the invention provides a preparation method of a phytosterol nano-preparation, which comprises the following steps:
(1) Dissolving maltodextrin and protein in deionized water to obtain a water phase for later use;
(2) Adding the phytosterol and the emulsifying agent into ethanol, and stirring until the phytosterol and the emulsifying agent are completely dissolved to obtain an organic phase for later use;
(3) Mixing the aqueous phase in the step (1) with the organic phase in the step (2), and then sequentially carrying out homogenization and ultrasonic treatment to obtain mixed emulsion;
(4) And (3) removing ethanol in the mixed emulsion in the step (3), and then performing freeze drying to obtain the plant sterol nano preparation.
Further, the protein of step (1) is ovotransferrin.
Further, the mass ratio of the maltodextrin to the protein in the step (1) is 1:1-8, and the mass volume ratio of the total amount of the maltodextrin and the protein to the deionized water is 1-3mg/mL.
Further, the dissolving method in the step (1) is to add maltodextrin into deionized water, stir until the maltodextrin is completely dissolved, then add protein under stirring, and stir until the maltodextrin is completely dissolved.
Further, the plant sterol in the step (2) is soybean plant sterol, the emulsifier comprises a natural emulsifier or a synthetic emulsifier, preferably one or more of tween 40, castor oil and span 20, and the emulsifier has the advantages of good emulsifying function property, safety and reliability.
Further, the mass ratio of the plant sterol to the emulsifier in the step (2) is 1:1-5, and the mass volume ratio of the emulsifier to the ethanol is 10-50mg/mL.
Further, the stirring temperature in the step (2) is 80-100 ℃, and the stirring time is 1-5min.
Further, the volume ratio of the water phase to the organic phase in the step (3) is 10:1-3, the homogenizing rotating speed is 3000-10000rpm, the homogenizing time is 3-8min, the ultrasonic power is 400W, and the ultrasonic time is 5-15min.
Further, the ethanol is removed in the step (4) by rotary evaporation, the temperature of the rotary evaporation is 40-50 ℃, the vacuum degree is 0.04-0.1MPa, the mixed emulsion with the ethanol removed is diluted to the mass before the rotary evaporation by deionized water before the freeze drying, and the freeze drying time is 24-48 hours.
The phytosterol nanometer preparation prepared by the preparation method is provided.
The invention also provides application of the phytosterol nano-preparation in preparation of functional foods and medicines.
Compared with the prior art, the invention has the following beneficial effects: the invention adopts maltodextrin and the egg transferrin as wall materials to embed the phytosterol, wherein the egg transferrin has better interfacial activity, can be used as a natural emulsifier to improve the emulsifying effect on the phytosterol, thereby reducing the particle size of the nano-preparation of the phytosterol, and simultaneously has various biological characteristics such as antioxidation, antibiosis, anti-tumor, anti-inflammatory and the like; the maltodextrin has the characteristics of good emulsifying effect and thickening effect, good solubility, excellent filling effect, difficult moisture absorption, difficult agglomeration, good carrier property, good stability and the like, can improve the dispersibility of the phytosterol nano-preparation, and simultaneously contains a large amount of polysaccharide bioactive substances, and trace elements such as calcium, iron and the like and mineral substances beneficial to human bodies.
In addition, the prepared nano phytosterol preparation has high embedding rate, good re-solubility and dispersibility and high bioavailability, and can well improve the application of the phytosterol in foods and medicines.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the invention, are within the scope of the invention.
The test methods or test methods described in the following examples are all conventional methods unless otherwise specified; the starting materials and auxiliaries, unless otherwise specified, are obtained commercially from conventional sources or are prepared in conventional manner.
Example 1
The preparation method of the phytosterol nano preparation comprises the following specific steps:
(1) Adding maltodextrin into deionized water, stirring until the maltodextrin is completely dissolved, slowly adding egg transferrin under a stirring state, and stirring and dissolving uniformly to obtain a water phase, wherein the mass ratio of the maltodextrin to the egg transferrin is 1:4, and the mass volume ratio of the total amount of the maltodextrin and the egg transferrin to the deionized water is 2mg/mL;
(2) Adding soyasterol, tween 40 and castor oil into ethanol, and magnetically stirring at 100deg.C for 2min to fully dissolve to obtain organic phase, wherein the mass ratio of soyasterol to emulsifier (Tween 40 and castor oil) is 1:4, the mass ratio of Tween 40 to castor oil is 1:1, and the mass volume ratio of emulsifier (Tween 40 and castor oil) to ethanol is 25mg/mL;
(3) Mixing the aqueous phase in the step (1) with the organic phase in the step (2), wherein the volume ratio of the aqueous phase to the organic phase is 10:1, then carrying out homogenization treatment under the condition of 5000rpm, wherein the homogenization time is 5min, and then carrying out ultrasonic treatment under the condition of 400W, wherein the ultrasonic time is 10mm, so as to obtain mixed emulsion;
(4) And (3) placing the mixed emulsion in a distillation flask, performing rotary evaporation at 45 ℃ and a vacuum degree of 0.09MPa to remove ethanol, diluting the product after rotary evaporation to the mass before rotary evaporation by deionized water, and performing freeze drying for 24 hours to obtain the phytosterol nano-preparation.
Example 2
The preparation method of the phytosterol nano preparation comprises the following specific steps:
(1) Adding maltodextrin into deionized water, stirring until the maltodextrin is completely dissolved, slowly adding egg transferrin under a stirring state, and stirring and dissolving uniformly to obtain a water phase, wherein the mass ratio of the maltodextrin to the egg transferrin is 1:4, and the mass volume ratio of the total amount of the maltodextrin and the egg transferrin to the deionized water is 2mg/mL;
(2) Adding soyasterol and Tween 40 into ethanol, and magnetically stirring at 100deg.C for 2min to fully dissolve to obtain organic phase, wherein the mass ratio of soyasterol and Tween 40 is 1:3, and the mass volume ratio of Tween 40 and ethanol is 20mg/mL;
(3) Mixing the aqueous phase in the step (1) with the organic phase in the step (2), wherein the volume ratio of the aqueous phase to the organic phase is 10:1, then carrying out homogenization treatment under the condition of 5000rpm, wherein the homogenization time is 5min, and then carrying out ultrasonic treatment under the condition of 400W, wherein the ultrasonic time is 10mm, so as to obtain mixed emulsion;
(4) And (3) placing the mixed emulsion in a distillation flask, performing rotary evaporation at 45 ℃ and a vacuum degree of 0.09MPa to remove ethanol, diluting the product after rotary evaporation to the mass before rotary evaporation by deionized water, and performing freeze drying for 24 hours to obtain the phytosterol nano-preparation.
Example 3
The preparation method of the phytosterol nano preparation comprises the following specific steps:
(1) Adding maltodextrin into deionized water, stirring until the maltodextrin is completely dissolved, slowly adding egg transferrin under a stirring state, and stirring and dissolving uniformly to obtain a water phase, wherein the mass ratio of the maltodextrin to the egg transferrin is 1:2, and the mass volume ratio of the total amount of the maltodextrin and the egg transferrin to the deionized water is 2mg/mL;
(2) Adding soybean phytosterol, tween 40 and span 20 into ethanol, and magnetically stirring at 100deg.C for 2min to fully dissolve to obtain organic phase, wherein the mass ratio of soybean phytosterol to emulsifier (tween 40 and span 20) is 1:3, the mass ratio of tween 40 to span 20 is 2:1, and the mass volume ratio of emulsifier (tween 40 and span 20) to ethanol is 20mg/mL;
(3) Mixing the aqueous phase in the step (1) with the organic phase in the step (2), wherein the volume ratio of the aqueous phase to the organic phase is 10:1, then carrying out homogenization treatment under the condition of 5000rpm, wherein the homogenization time is 5min, and then carrying out ultrasonic treatment under the condition of 400W, wherein the ultrasonic time is 10mm, so as to obtain mixed emulsion;
(4) And (3) placing the mixed emulsion in a distillation flask, performing rotary evaporation at 45 ℃ and a vacuum degree of 0.09MPa to remove ethanol, diluting the product after rotary evaporation to the mass before rotary evaporation by deionized water, and performing freeze drying for 24 hours to obtain the phytosterol nano-preparation.
Comparative example 1
The difference from example 3 is that the ovotransferrin in step (1) is replaced with soy protein isolate and the rest of the procedure is the same as example 3.
Comparative example 2
The difference from example 3 is that maltodextrin in step (1) was omitted and the rest of the steps were the same as in example 3.
The phytosterol nanoformulations prepared in examples 1-3 and comparative examples 1-2 were tested for performance using the following test methods:
1) The embedding rate of the prepared phytosterol nano-preparation is measured by adopting a gas chromatography method, wherein the embedding rate is = (1-the amount of surface phytosterol/the amount of total phytosterol) multiplied by 100%;
2) Measuring the particle size and PDI of the prepared phytosterol nano-preparation by using a particle size potentiometer;
3) The method for measuring the bioavailability comprises the following steps: obtaining a phytosterol nano-preparation digestive juice according to an in vitro digestion model in Fei-Ping Chen,Bian-Sheng Li,Chuan-He Tang.Nanocomplexation between curcumin and soy protein isolate:influence on curcumin stability/bioaccessibility and in vitro protein digestibility.Journal of Agricultural and Food Chemistry,2015,63(13),3559-3569., taking 1mL of the phytosterol nano-preparation digestive juice into a centrifuge tube, centrifuging for 30min at 10000g and 10 ℃, taking supernatant to determine the amount of the phytosterol, and the bioavailability = the amount of the phytosterol in the supernatant/the amount of the total phytosterol multiplied by 100%;
4) Method for measuring solubility: dissolving a certain amount of the phytosterol nano-preparation in water to enable a sample to reach a supersaturated state, sufficiently oscillating for 10min, oscillating for 24h at 37 ℃ by a shaking table, centrifuging for 10min at 10000rpm, taking supernatant, and measuring the phytosterol content in the supernatant by gas chromatography-mass spectrometry, wherein the solubility=the phytosterol content (mg)/the solution volume (mL) in the supernatant.
The test results are shown in the following table.
The experimental data show that the plant sterol nano-preparation prepared in the examples 1-3 has good embedding rate, re-solubility, dispersibility and bioavailability, and can effectively improve the water solubility of plant sterols.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. The preparation method of the phytosterol nano-preparation is characterized by comprising the following steps:
(1) Dissolving maltodextrin and protein in deionized water to obtain a water phase for later use;
(2) Adding the phytosterol and the emulsifying agent into ethanol, and stirring until the phytosterol and the emulsifying agent are completely dissolved to obtain an organic phase for later use;
(3) Mixing the aqueous phase in the step (1) with the organic phase in the step (2), and then sequentially carrying out homogenization and ultrasonic treatment to obtain mixed emulsion;
(4) And (3) removing ethanol in the mixed emulsion in the step (3), and then performing freeze drying to obtain the plant sterol nano preparation.
2. The method of claim 1, wherein the protein of step (1) is ovotransferrin.
3. The method for preparing the nano-preparation of plant sterol according to claim 1, wherein the mass ratio of maltodextrin to protein in the step (1) is 1:1-8, and the mass volume ratio of the total amount of maltodextrin and protein to deionized water is 1-3mg/mL.
4. The method for preparing the nano-preparation of plant sterol according to claim 1, wherein the dissolving mode in the step (1) is to add maltodextrin into deionized water, stir until the maltodextrin is completely dissolved, then add protein under stirring, and stir until the maltodextrin is completely dissolved.
5. The method for preparing the plant sterol nano-preparation according to claim 1, wherein the plant sterol in the step (2) is soybean plant sterol, the emulsifying agent is one or more of tween 40, castor oil and span 20, the mass ratio of the plant sterol to the emulsifying agent is 1:1-5, and the mass volume ratio of the emulsifying agent to the ethanol is 10-50mg/mL.
6. The method for preparing a nano-preparation of plant sterol according to claim 1, wherein the stirring temperature in the step (2) is 80-100 ℃ and the stirring time is 1-5min.
7. The method for preparing the nano-preparation of plant sterol according to claim 1, wherein the volume ratio of the aqueous phase to the organic phase in the step (3) is 10:1-3, the homogenizing speed is 3000-10000rpm, the homogenizing time is 3-8min, the power of the ultrasonic wave is 400W, and the ultrasonic wave time is 5-15min.
8. The method for preparing the nano-preparation of plant sterol according to claim 1, wherein the ethanol removal mode in the step (4) is rotary evaporation, the temperature of the rotary evaporation is 40-50 ℃, the vacuum degree is 0.04-0.1MPa, the mixed emulsion with ethanol removed is diluted with deionized water to the mass before the rotary evaporation before the freeze drying, and the freeze drying time is 24-48h.
9. A phytosterol nano-formulation produced by the method of any one of claims 1-8.
10. Use of the phytosterol nano formulation of claim 9 in the preparation of functional foods and medicines.
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