CN105124603A - Phytosterol-protein compound, as well as preparation and application thereof - Google Patents
Phytosterol-protein compound, as well as preparation and application thereof Download PDFInfo
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- CN105124603A CN105124603A CN201510600660.8A CN201510600660A CN105124603A CN 105124603 A CN105124603 A CN 105124603A CN 201510600660 A CN201510600660 A CN 201510600660A CN 105124603 A CN105124603 A CN 105124603A
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Abstract
The invention belongs to the technical field of functional food processing, and discloses a phytosterol-protein compound, as well as preparation and application thereof. The preparation method comprises the following steps: dispersing and dissolving protein in an aqueous phase according to a solid-to-liquid ratio of (0.1-0.5):10, and hydrating the liquid at 0-10 DEG C for 6-10 hours to obtain a protein solution; dissolving phytosterol in absolute ethanol to obtain a phytosterol ethanol solution according to a solid-to-liquid ratio of (5-14):1; adding the phytosterol ethanol solution into the protein solution under a stirring condition according to a volume ratio of (10-30):100, and uniformly stirring and mixing to obtain a mixed liquid; distilling the mixed liquid to remove the absolute ethanol in the mixed liquid, freezing and drying to obtain the phytosterol-protein compound. The preparation method is simple, low in cost, safe and environment-friendly, and the compound is capable of greatly improving the water solubility and bioavailability of phytosterol and can be used for developing functional health-care foods.
Description
Technical field
The invention belongs to functional food processing technique field, be specifically related to one plant sterols-protein complex and preparation and application.
Background technology
Phytosterol is a kind of cholesterol analog, is classified as functional grease, and having the character such as crystallinity, oily slightly solubility, water-insoluble, is a kind of important food function factor, has anti-inflammatory, anti-oxidant, especially significantly can reduce effect of cholesterol.In recent years, along with improving constantly and development to phytosterol Study on Physiological Effects of health of people consciousness, that particularly be familiar with the health care of phytosterol deepens constantly, and makes phytosterol research and development become focus gradually.But because phytosterol is water insoluble, oil-soluble difference and easily the shortcoming such as crystallization cause its bioavailability extremely low, become and affect its bottleneck in industrial development.Therefore, find practicable method and carry out modification to phytosterol, make it can either retain original biologically active, also can be widely used in food industry system, be current problem demanding prompt solution simultaneously.
At present, domestic and international many scholar's developmental researches method of multiple high load phytosterol.These methods mainly concentrate on the oil-soluble modification of phytosterol, utilize enzyme process and chemical method to carry out esterification to it, improve its oil-soluble, and then can be applied to some high-fat foods.But along with the too much absorption of oil-soluble food, will certainly bring same health problem, this is that we are reluctant to see.Therefore, phytosterol is carried out to the modification of water-soluble aspect, be applied to low fat food as beverage, in the production of milk etc., can yet be regarded as one and improve the good method of phytosterol bio utilization rate.
Summary of the invention
In order to solve the shortcoming and defect part of above prior art, primary and foremost purpose of the present invention is the preparation method providing one plant sterols-protein complex.
Another object of the present invention is to provide a kind of phytosterol-protein complex prepared by said method.
Another object of the present invention is to provide the application of above-mentioned phytosterol-protein complex in functional health-care food.
The object of the invention is achieved through the following technical solutions:
The preparation method of one plant sterols-protein complex, comprises following preparation process:
(1) be (0.1 ~ 0.5) according to solid-to-liquid ratio (kilogram: rise): protein dispersibility is dissolved in aqueous phase by the ratio of 10, be then placed at 0 ~ 10 DEG C of temperature aquation 6 ~ 10 hours, obtain protein solution;
(2) be (5 ~ 14) according to solid-to-liquid ratio (gram: rise): phytosterol to be dissolved in absolute ethyl alcohol and to obtain phytosterol ethanolic solution by 1;
(3), under stirring condition, be (10 ~ 30) by volume: phytosterol ethanolic solution joins in protein solution by 100, is uniformly mixed to obtain mixed liquor;
(4) the absolute ethyl alcohol steaming in step (3) gained mixed liquor removed, freeze drying, obtains phytosterol-protein complex.
Solid-to-liquid ratio described in step (1) is preferably (0.2 ~ 0.4): 10.
Described protein preferably has the protein of strong absorption or complexing power, as soybean protein isolate, lactalbumin or casein sodium.
Solid-to-liquid ratio described in step (2) is preferably (8 ~ 12): 1.
Volume ratio described in step (3) is preferably (10 ~ 20): 100.
Before freeze drying described in step (4), also add deionized water and regulate concentration; The amount of adding deionized water is preferably the amount of the deionized water of steaming except the loss of absolute ethyl alcohol institute.
Before freeze drying described in step (4), also by the free phytosterol of centrifugal removing and protein, the stability of improving product and purity.
One plant sterols-protein complex, is prepared by above method.
The application of above-mentioned phytosterol-protein complex in functional health-care food.
Preparation method of the present invention and the product tool obtained have the following advantages and beneficial effect:
(1) the present invention utilizes the amphipathic characteristic of protein, and by special preparation technology, effectively by completely hydrophobic phytosterol high dispersive in aqueous phase, form nanoscale albumen composition, can greatly improve the water-soluble of phytosterol and bioavailability, and overcome the easy crystallisation problems that phytosterol occurs in actual production process;
(2) product of the present invention not only can be applied to health product as food function factor, also has the great potential as Cardiovarscular reagent combination;
(3) production technology involved by preparation method of the present invention is simple, is a kind of green, safety, is suitable for the production application of food, pharmaceutical industries.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
(1) under room temperature, according to solid-to-liquid ratio (kilogram: rise) for soybean isolate protein powder end is scattered in aqueous phase by the ratio of 0.25:10, under the effect of magnetic stirrer, soybean protein isolate is dissolved completely, then aquation is placed in the refrigerator of 4 DEG C 8 hours, then centrifugal (8000r, 25min) removes insoluble matter, obtains protein solution;
(2) according to solid-to-liquid ratio (gram: rise), for phytosterol is dissolved in absolute ethyl alcohol by 10:1, then centrifugal (8000r, 25min) removes insoluble matter, obtains the phytosterol ethanolic solution that concentration is 10g/L;
(3) under room temperature and stirring condition, dropwise joined in protein solution by phytosterol ethanolic solution by volume for 1:5, continuation stirring 3h mixes and obtains mixed liquor;
(4) with Rotary Evaporators, the absolute ethyl alcohol steaming in step (3) gained mixed liquor is removed, then interpolation deionized water is consistent with the protein solution concentration before evaporation after making evaporation, by centrifugal for mixed liquor (8000r, the phytosterol that 25min) removing is free and soybean protein isolate, obtain phytosterol-soybean protein isolate complex solution, again through freeze drying, obtain phytosterol-soybean protein isolate composite powder.
It is faint yellow homogeneous powder that the present embodiment obtains phytosterol-soybean protein isolate composite powder, after measured, its envelop rate is 86.79%, and capacity value is every gram of albumen load 69.432mg phytosterol, and the solubility of phytosterol increases to 1.736g/L by original water-insoluble.Before measuring this compound drying by nano particle size instrument, the average grain diameter of solution and the rear solution of redissolution is respectively 174nm and 179nm, illustrates that this compound has good heavy dispersiveness.Carry out shed storage test to this complex solution, there is not crystallization and precipitation in result solution after room temperature places three weeks, illustrates that this compound has storage-stable.In vitro digestion is carried out to this composite powder, finds that the bioavailability of phytosterol increases to 86.42% by 51.99%.Above data can show, phytosterol of the present invention-soybean protein isolate compound effectively can improve water-soluble, stability and the biological transformation ratio of phytosterol.
Embodiment 2
(1) under room temperature, according to solid-to-liquid ratio (kilogram: rise) for lactalbumin powder is scattered in aqueous phase by the ratio of 0.1:10, under the effect of magnetic stirrer, lactalbumin is dissolved completely, then aquation is placed in the refrigerator of 4 DEG C 8 hours, then centrifugal (8000r, 25min) removes insoluble matter, obtains protein solution;
(2) according to solid-to-liquid ratio (gram: rise), for phytosterol is dissolved in absolute ethyl alcohol by 8:1, then centrifugal (8000r, 25min) removes insoluble matter, obtains the phytosterol ethanolic solution that concentration is 10g/L;
(3) under room temperature and stirring condition, dropwise joined in protein solution by phytosterol ethanolic solution by volume for 2:25, continuation stirring 5h mixes and obtains mixed liquor;
(4) with Rotary Evaporators, the absolute ethyl alcohol steaming in step (3) gained mixed liquor is removed, then interpolation deionized water is consistent with the protein solution concentration before evaporation after making evaporation, by centrifugal for mixed liquor (8000r, the phytosterol that 25min) removing is free and lactalbumin, obtain phytosterol-lactalbumin complex solution, again through freeze drying, obtain phytosterol-lactalbumin complex powder.
The phytosterol that the present embodiment obtains-lactalbumin complex powder is light yellow homogeneous powder, after measured, its envelop rate is 97.99%, and capacity value is every gram of albumen load 62.714mg phytosterol, and the solubility of phytosterol increases to 0.627g/L by original water-insoluble.Before measuring this compound drying by nano particle size instrument, the average grain diameter of solution and the rear solution of redissolution is respectively 187nm and 189nm, illustrates that this compound has good heavy dispersiveness.Carry out shed storage test to this complex solution, there is not crystallization and precipitation in result solution after room temperature places three weeks, illustrates that this compound has storage-stable.In vitro digestion is carried out to this composite powder, finds that the bioavailability of phytosterol increases to 73 by 51.99%, 88%.Above data can show, phytosterol-lactalbumin complex of the present invention effectively can improve water-soluble, stability and the biological transformation ratio of phytosterol.
Embodiment 3
(1) under room temperature, according to solid-to-liquid ratio (kilogram: rise) for sodium caseinate powder is scattered in aqueous phase by the ratio of 0.25:10, under the effect of magnetic stirrer, sodium caseinate powder is dissolved completely, then aquation is placed in the refrigerator of 4 DEG C 8 hours, then centrifugal (8000r, 25min) removes insoluble matter, obtains protein solution;
(2) according to solid-to-liquid ratio (gram: rise), for phytosterol is dissolved in absolute ethyl alcohol by 10:1, then centrifugal (8000r, 25min) removes insoluble matter, obtains the phytosterol ethanolic solution that concentration is 10g/L;
(3) under room temperature and stirring condition, dropwise joined in protein solution by phytosterol ethanolic solution by volume for 1:5, continuation stirring 3h mixes and obtains mixed liquor;
(4) with Rotary Evaporators, the absolute ethyl alcohol steaming in step (3) gained mixed liquor is removed, then interpolation deionized water is consistent with the protein solution concentration before evaporation after making evaporation, by centrifugal for mixed liquor (8000r, the phytosterol that 25min) removing is free and casein sodium, obtain phytosterol-casein sodium complex solution, again through freeze drying, obtain phytosterol-casein sodium compound.
The phytosterol that the present embodiment obtains-casein sodium compound is the chip solid of white, after measured, its envelop rate is 79.86%, and capacity value is every gram of albumen load 63.888mg phytosterol, and the solubility of phytosterol increases to 1.598g/L by original water-insoluble.Before measuring this compound drying by nano particle size instrument, the average grain diameter of solution and the rear solution of redissolution is respectively 195nm and 201nm, illustrates that this compound has good heavy dispersiveness.Carry out shed storage test to this complex solution, there is not crystallization and precipitation in result solution after room temperature places three weeks, illustrates that this compound has storage-stable.In vitro digestion is carried out to this composite powder, finds that the bioavailability of phytosterol increases to 91.66% by 51.99%.Above data can show, phytosterol of the present invention-casein sodium compound effectively can improve water-soluble, stability and the biological transformation ratio of phytosterol.
Embodiment 4
(1) under room temperature, according to solid-to-liquid ratio (kilogram: rise) for soybean isolate protein powder end is scattered in aqueous phase by the ratio of 0.4:10, under the effect of magnetic stirrer, soybean isolate protein powder end is dissolved completely, then aquation is placed in the refrigerator of 4 DEG C 8 hours, then centrifugal (8000r, 25min) removes insoluble matter, obtains protein solution;
(2) according to solid-to-liquid ratio (gram: rise), for phytosterol is dissolved in absolute ethyl alcohol by 12:1, then centrifugal (8000r, 25min) removes insoluble matter, obtains the phytosterol ethanolic solution that concentration is 12g/L;
(3) under room temperature and stirring condition, dropwise joined in protein solution by phytosterol ethanolic solution by volume for 1:10, continuation stirring 3h mixes and obtains mixed liquor;
(4) with Rotary Evaporators, the absolute ethyl alcohol steaming in step (3) gained mixed liquor is removed, then interpolation deionized water is consistent with the protein solution concentration before evaporation after making evaporation, by centrifugal for mixed liquor (8000r, the phytosterol that 25min) removing is free and soybean protein isolate, obtain complex solution, again through freeze drying, obtain phytosterol-soybean protein isolate compound.
The phytosterol that the present embodiment obtains-soybean protein isolate compound is flaxen powder solid, after measured, its envelop rate is 93.79%, and capacity value is every gram of albumen load 28.13mg phytosterol, and the solubility of phytosterol increases to 1.125g/L by original water-insoluble.Before measuring this compound drying by nano particle size instrument, the average grain diameter of solution and the rear solution of redissolution is respectively 171nm and 177nm, illustrates that this compound has good heavy dispersiveness.Carry out shed storage test to this complex solution, there is not crystallization and precipitation in result solution after room temperature places three weeks, illustrates that this compound has storage-stable.In vitro digestion is carried out to this composite powder, finds that the bioavailability of phytosterol increases to 90.84% by 51.99%.Above data can show, phytosterol of the present invention-casein sodium compound effectively can improve water-soluble, stability and the biological transformation ratio of phytosterol.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. the preparation method of one plant sterols-protein complex, is characterized in that, comprises following preparation process:
(1) be (0.1 ~ 0.5) according to solid-to-liquid ratio: protein dispersibility is dissolved in aqueous phase by the ratio of 10, be then placed at 0 ~ 10 DEG C of temperature aquation 6 ~ 10 hours, obtain protein solution;
(2) be (5 ~ 14) according to solid-to-liquid ratio: phytosterol to be dissolved in absolute ethyl alcohol and to obtain phytosterol ethanolic solution by 1;
(3), under stirring condition, be (10 ~ 30) by volume: phytosterol ethanolic solution joins in protein solution by 100, is uniformly mixed to obtain mixed liquor;
(4) the absolute ethyl alcohol steaming in step (3) gained mixed liquor removed, freeze drying, obtains phytosterol-protein complex.
2. the preparation method of one plant sterols according to claim 1-protein complex, is characterized in that: the solid-to-liquid ratio described in step (1) is (0.2 ~ 0.4): 10.
3. the preparation method of one plant sterols according to claim 1-protein complex, is characterized in that: described protein is soybean protein isolate, lactalbumin or casein sodium.
4. the preparation method of one plant sterols according to claim 1-protein complex, is characterized in that: the solid-to-liquid ratio described in step (2) is (8 ~ 12): 1.
5. the preparation method of one plant sterols according to claim 1-protein complex, is characterized in that: the volume ratio described in step (3) is (10 ~ 20): 100.
6. the preparation method of one plant sterols according to claim 1-protein complex, is characterized in that: before the freeze drying described in step (4), also adds deionized water and regulates concentration.
7. the preparation method of one plant sterols according to claim 6-protein complex, is characterized in that: the addition of deionized water removes the amount of the deionized water of absolute ethyl alcohol institute loss for steaming.
8. the preparation method of one plant sterols according to claim 1-protein complex, it is characterized in that: before the freeze drying described in step (4), also by the free phytosterol of centrifugal removing and protein, the stability of improving product and purity.
9. one plant sterols-protein complex, is characterized in that: prepared by the method described in any one of claim 1 ~ 8.
10. the application of phytosterol-protein complex according to claim 9 in functional health-care food.
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Cited By (9)
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| CN107568744A (en) * | 2017-08-21 | 2018-01-12 | 华南理工大学 | Heat treatment combines the method that the processing of high pressure microjet prepares stable type soybean protein sterol particles |
| CN108606952A (en) * | 2018-05-15 | 2018-10-02 | 浙江工业大学 | A kind of preparation method of the nanoemulsions of zeins load phytosterol |
| CN109511772A (en) * | 2018-11-26 | 2019-03-26 | 华南理工大学 | A kind of dissolution, synergy indissoluble bioactive substance albumen base nano particle preparation method |
| CN110192651A (en) * | 2019-05-31 | 2019-09-03 | 浙江工业大学 | A kind of preparation method of the nanoemulsions using soybean protein isolate load phytosterin ester |
| CN110279123A (en) * | 2019-07-31 | 2019-09-27 | 江南大学 | A kind of preparation method of water-soluble plant sterols nano-hydrogel lotion |
| CN111213880A (en) * | 2019-12-16 | 2020-06-02 | 华南理工大学 | Soybean protein-based nano curcumin suitable for research and development of functional health food for preventing intestinal cancer and production method thereof |
| CN113854576A (en) * | 2021-09-23 | 2021-12-31 | 江南大学 | Preparation method of sodium caseinate-pectin-phytosterol nanoparticles |
| CN114403449A (en) * | 2022-01-19 | 2022-04-29 | 浙江大学 | Protein-sterol covalent complex and preparation method and application thereof |
| CN118141106A (en) * | 2024-03-08 | 2024-06-07 | 时代生物科技（深圳）有限公司 | Plant sterol nano preparation and preparation method and application thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568744A (en) * | 2017-08-21 | 2018-01-12 | 华南理工大学 | Heat treatment combines the method that the processing of high pressure microjet prepares stable type soybean protein sterol particles |
| CN107568744B (en) * | 2017-08-21 | 2020-12-22 | 华南理工大学 | Method for preparing stable soybean protein-sterol particles by heat treatment combined with high-pressure micro-jet treatment |
| CN108606952A (en) * | 2018-05-15 | 2018-10-02 | 浙江工业大学 | A kind of preparation method of the nanoemulsions of zeins load phytosterol |
| CN109511772A (en) * | 2018-11-26 | 2019-03-26 | 华南理工大学 | A kind of dissolution, synergy indissoluble bioactive substance albumen base nano particle preparation method |
| CN109511772B (en) * | 2018-11-26 | 2021-09-21 | 华南理工大学 | Preparation method of protein-based nanoparticles of dissolution-promoting and synergistic insoluble bioactive substances |
| CN110192651A (en) * | 2019-05-31 | 2019-09-03 | 浙江工业大学 | A kind of preparation method of the nanoemulsions using soybean protein isolate load phytosterin ester |
| CN110279123A (en) * | 2019-07-31 | 2019-09-27 | 江南大学 | A kind of preparation method of water-soluble plant sterols nano-hydrogel lotion |
| CN111213880A (en) * | 2019-12-16 | 2020-06-02 | 华南理工大学 | Soybean protein-based nano curcumin suitable for research and development of functional health food for preventing intestinal cancer and production method thereof |
| CN113854576A (en) * | 2021-09-23 | 2021-12-31 | 江南大学 | Preparation method of sodium caseinate-pectin-phytosterol nanoparticles |
| CN114403449A (en) * | 2022-01-19 | 2022-04-29 | 浙江大学 | Protein-sterol covalent complex and preparation method and application thereof |
| CN114403449B (en) * | 2022-01-19 | 2023-05-09 | 浙江大学 | Protein-sterol covalent complex and preparation method and application thereof |
| CN118141106A (en) * | 2024-03-08 | 2024-06-07 | 时代生物科技（深圳）有限公司 | Plant sterol nano preparation and preparation method and application thereof |
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