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CN117881793A - 病原目标的环介导等温扩增(lamp)分析 - Google Patents

病原目标的环介导等温扩增(lamp)分析 Download PDF

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Publication number
CN117881793A
CN117881793A CN202280021474.7A CN202280021474A CN117881793A CN 117881793 A CN117881793 A CN 117881793A CN 202280021474 A CN202280021474 A CN 202280021474A CN 117881793 A CN117881793 A CN 117881793A
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CN
China
Prior art keywords
lamp
composition
saliva
reaction
solid phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280021474.7A
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English (en)
Chinese (zh)
Inventor
J·塞维利亚
D·麦克切斯尼
J·戴维森
J·王
M·K·玛鲁塔木图
A·德克斯特
M·维尔马
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Raytheon BBN Technologies Corp
Purdue Research Foundation
Original Assignee
Raytheon BBN Technologies Corp
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Raytheon BBN Technologies Corp, Purdue Research Foundation filed Critical Raytheon BBN Technologies Corp
Publication of CN117881793A publication Critical patent/CN117881793A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30Oligonucleotides characterised by their secondary structure
    • C12Q2525/301Hairpin oligonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202280021474.7A 2021-01-15 2022-01-15 病原目标的环介导等温扩增(lamp)分析 Pending CN117881793A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163138312P 2021-01-15 2021-01-15
US63/138,312 2021-01-15
PCT/US2022/012635 WO2022155546A1 (en) 2021-01-15 2022-01-15 Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets

Publications (1)

Publication Number Publication Date
CN117881793A true CN117881793A (zh) 2024-04-12

Family

ID=80222449

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280021474.7A Pending CN117881793A (zh) 2021-01-15 2022-01-15 病原目标的环介导等温扩增(lamp)分析

Country Status (12)

Country Link
EP (1) EP4278001A1 (es)
JP (1) JP2024502639A (es)
KR (1) KR20230126217A (es)
CN (1) CN117881793A (es)
AR (1) AR124656A1 (es)
AU (1) AU2022207503A1 (es)
BR (1) BR112023014265A2 (es)
CA (1) CA3203454A1 (es)
CL (1) CL2023002063A1 (es)
MX (1) MX2023008416A (es)
TW (1) TW202242131A (es)
WO (1) WO2022155546A1 (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11746372B2 (en) * 2017-12-01 2023-09-05 Godx, Inc. Rapid nucleic acids separation and sample preparation via hollow-centered silica microsphere
CN111500778A (zh) * 2020-04-30 2020-08-07 三亚市人民医院 逆转录环介导等温扩增结合金纳米生物传感检测2019新型冠状病毒的方法

Also Published As

Publication number Publication date
KR20230126217A (ko) 2023-08-29
TW202242131A (zh) 2022-11-01
MX2023008416A (es) 2024-04-29
EP4278001A1 (en) 2023-11-22
JP2024502639A (ja) 2024-01-22
AR124656A1 (es) 2023-04-19
AU2022207503A1 (en) 2023-06-22
CA3203454A1 (en) 2022-07-21
WO2022155546A1 (en) 2022-07-21
BR112023014265A2 (pt) 2023-10-24
CL2023002063A1 (es) 2024-04-19

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