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AR124656A1 - Análisis de amplificación isotérmica mediada por horquillas (lamp) para objetivos patógenos - Google Patents

Análisis de amplificación isotérmica mediada por horquillas (lamp) para objetivos patógenos

Info

Publication number
AR124656A1
AR124656A1 ARP220100080A ARP220100080A AR124656A1 AR 124656 A1 AR124656 A1 AR 124656A1 AR P220100080 A ARP220100080 A AR P220100080A AR P220100080 A ARP220100080 A AR P220100080A AR 124656 A1 AR124656 A1 AR 124656A1
Authority
AR
Argentina
Prior art keywords
solid phase
phase medium
lamp
composition
analysis
Prior art date
Application number
ARP220100080A
Other languages
English (en)
Inventor
Jordan Seville
Darby Mchesney
Josiah Davidson
Jiangshan Wang
Murali Kannan Maruthamuthu
Andres Dextre
Mohit Verma
Original Assignee
Purdue Research Foundation
Raytheon Bbn Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation, Raytheon Bbn Technologies Corp filed Critical Purdue Research Foundation
Publication of AR124656A1 publication Critical patent/AR124656A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente divulgación se refiere a composiciones, métodos y sistemas para el análisis de amplificación isotérmica mediada por horquillas (LAMP) en un medio en fase sólida. La composición puede comprender uno o más cebadores objetivo, un DNA polimerasa y un agente de resolubilización. La composición puede estar sustancialmente libre de agentes no sensibles al pH capaces de decolorar el medio en fase sólida. El método puede comprender proporcionar un conjunto de un medio en fase sólida, depositar una muestra biológica en el medio en fase sólida y calentar el conjunto a una temperatura isotérmica suficiente para facilitar una reacción de LAMP. El sistema puede comprender una composición y un medio en fase sólida sobre el que se deposita la composición.
ARP220100080A 2021-01-15 2022-01-17 Análisis de amplificación isotérmica mediada por horquillas (lamp) para objetivos patógenos AR124656A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163138312P 2021-01-15 2021-01-15

Publications (1)

Publication Number Publication Date
AR124656A1 true AR124656A1 (es) 2023-04-19

Family

ID=80222449

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220100080A AR124656A1 (es) 2021-01-15 2022-01-17 Análisis de amplificación isotérmica mediada por horquillas (lamp) para objetivos patógenos

Country Status (12)

Country Link
EP (1) EP4278001A1 (es)
JP (1) JP2024502639A (es)
KR (1) KR20230126217A (es)
CN (1) CN117881793A (es)
AR (1) AR124656A1 (es)
AU (1) AU2022207503A1 (es)
BR (1) BR112023014265A2 (es)
CA (1) CA3203454A1 (es)
CL (1) CL2023002063A1 (es)
MX (1) MX2023008416A (es)
TW (1) TW202242131A (es)
WO (1) WO2022155546A1 (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11746372B2 (en) * 2017-12-01 2023-09-05 Godx, Inc. Rapid nucleic acids separation and sample preparation via hollow-centered silica microsphere
CN111500778A (zh) * 2020-04-30 2020-08-07 三亚市人民医院 逆转录环介导等温扩增结合金纳米生物传感检测2019新型冠状病毒的方法

Also Published As

Publication number Publication date
KR20230126217A (ko) 2023-08-29
TW202242131A (zh) 2022-11-01
CN117881793A (zh) 2024-04-12
MX2023008416A (es) 2024-04-29
EP4278001A1 (en) 2023-11-22
JP2024502639A (ja) 2024-01-22
AU2022207503A1 (en) 2023-06-22
CA3203454A1 (en) 2022-07-21
WO2022155546A1 (en) 2022-07-21
BR112023014265A2 (pt) 2023-10-24
CL2023002063A1 (es) 2024-04-19

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