CN116970485A - Microorganism continuous fermentation integrated device - Google Patents
Microorganism continuous fermentation integrated device Download PDFInfo
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- CN116970485A CN116970485A CN202311158230.6A CN202311158230A CN116970485A CN 116970485 A CN116970485 A CN 116970485A CN 202311158230 A CN202311158230 A CN 202311158230A CN 116970485 A CN116970485 A CN 116970485A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 225
- 230000004151 fermentation Effects 0.000 title claims abstract description 224
- 244000005700 microbiome Species 0.000 title claims description 34
- 239000007788 liquid Substances 0.000 claims abstract description 77
- 238000003756 stirring Methods 0.000 claims abstract description 69
- 238000004140 cleaning Methods 0.000 claims abstract description 37
- 238000007790 scraping Methods 0.000 claims abstract description 29
- 230000000813 microbial effect Effects 0.000 claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims abstract description 17
- 238000001694 spray drying Methods 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 63
- 238000009826 distribution Methods 0.000 claims description 33
- 238000005253 cladding Methods 0.000 claims description 31
- 238000007789 sealing Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 238000004891 communication Methods 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 abstract description 21
- 238000005192 partition Methods 0.000 description 11
- 238000000034 method Methods 0.000 description 9
- 230000008859 change Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000009471 action Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000193171 Clostridium butyricum Species 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/087—Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/14—Drying
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Abstract
The utility model relates to the technical field of microbial fermentation, and particularly discloses a microbial continuous fermentation integrated device which comprises a first fermentation tank, a second fermentation tank, a buffer fermentation tank, a third fermentation tank, a buffer tank, a filtering device, a centrifugal concentration tank and a spray drying device, wherein the first fermentation tank is connected with the second fermentation tank; the first fermentation tank, the second fermentation tank, the buffer fermentation tank and the third fermentation tank are identical in structure and comprise tank bodies, each tank body comprises an outer shell and an inner shell, a driving motor is arranged at the top of each outer shell and connected with a stirring shaft, a stirring rod is arranged on the upper body of each stirring shaft, a movable scraping plate is arranged at the end part of each stirring rod, and a cleaning device is arranged on each scraping plate; a heating device is further arranged between the outer shell and the inner shell, and a heat source medium provided by the heating device can flow through a gap between the outer shell and the inner shell, the stirring shaft and the stirring rod so as to heat microbial liquid in the inner shell; the utility model can effectively improve the microbial fermentation culture efficiency and ensure that the bacterial liquid is heated more uniformly.
Description
Technical Field
The utility model relates to the technical field of microbial fermentation, in particular to a microbial continuous fermentation integrated device.
Background
Microbial fermentation is a process of converting raw materials into products required by human beings through specific metabolic pathways under proper conditions (such as proper temperature, air pressure and oxygen) by utilizing microorganisms, and is widely applied to the fields of medicine, food, chemistry, agriculture and the like; in most microbial fermentation processes, a plurality of single thalli are mixed and compounded according to a specific proportion and then fermented under a specific condition.
For example, the Chinese patent application with publication number of CN104371960A discloses a continuous fermentation method conforming to microbial agents and compound microorganisms adopted, and the technical scheme disclosed by the utility model comprises the following steps: 1) Respectively preparing bacillus subtilis bacterial liquid, bacillus licheniformis bacterial liquid and clostridium butyricum bacterial liquid; 2) Adding basic fermentation liquor into a fermentation tank; 3) Controlling the temperature of the basic fermentation liquid to be 36-38 ℃, controlling the pressure of the tank to be 0.01Mpa, firstly adding the bacillus subtilis liquid for fermentation culture, then adding the bacillus licheniformis liquid for continuous fermentation culture, and finally adding the clostridium butyricum liquid for standing anaerobic fermentation culture; 4) Filtering the fermentation liquor obtained in the step 3), and centrifuging and concentrating the fermentation liquor to 28-32% of the original volume; then spray drying to obtain a composite probiotic preparation; the utility model overcomes the defects of more sterilization times and more feeding times in the independent fermentation process of microorganisms, simplifies the fermentation process, and simultaneously improves the viable count, reduces the mixed bacteria count and improves the action effect of microorganisms by the synergistic effect of different microorganisms in continuous fermentation; however, the technical scheme disclosed by the utility model is that a plurality of fermentation procedures are carried out in the same fermentation tank, the time of each fermentation procedure is more than 10 hours, different strains are added for fermentation at specific time points according to the fermentation time of different monomer strains, the fermentation tank can only carry out the next microbial fermentation after the fermentation of the batch of microorganisms is finished, and the real continuous fermentation cannot be realized;
in the technical scheme that microorganisms are fermented and heated to control the fermentation temperature of the microorganisms, chinese patent publication No. CN206396195U discloses a high-efficiency heating microorganism aseptic fermentation tank, which is mainly characterized in that an infrared heating lamp tube is fixedly arranged in a stirring blade through a mounting frame so as to improve the heating efficiency and the heating uniformity; but can not provide heat source for other parts in the fermentation tank, and the microorganisms in the fermentation tank still can not be heated uniformly, so that the fermentation efficiency of the microorganisms is affected.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides a microorganism continuous fermentation integrated device, which solves the problems in the background art.
The technical scheme of the utility model is as follows:
a microorganism continuous fermentation integrated device comprises a first fermentation tank, at least one second fermentation tank, a buffer fermentation tank, at least one third fermentation tank, a buffer tank, a filtering device, a centrifugal concentration tank and a spray drying device which are sequentially connected through pipelines according to the microorganism fermentation process;
the structure of the first fermentation tank, the structure of the second fermentation tank, the structure of the buffer fermentation tank and the structure of the third fermentation tank are the same, the buffer fermentation tank and the third fermentation tank all comprise tank bodies, each tank body comprises an outer shell and an inner shell arranged in the outer shell, a driving motor is arranged at the top of each outer shell, an output shaft of each driving motor is connected with a stirring shaft extending to the bottom of each inner shell, a plurality of stirring rods are arranged on the whole body of each stirring shaft, scraping plates capable of moving along the length direction of each stirring rod are arranged at the end parts of the stirring rods, and cleaning devices are arranged on the scraping plates;
and a heating device is further arranged between the outer shell and the inner shell, and a heat source medium provided by the heating device can flow through a gap between the outer shell and the inner shell, the stirring shaft and the stirring rod so as to heat microbial liquid in the inner shell.
Further, the bottoms of the first fermentation tank, the second fermentation tank, the buffer fermentation tank and the third fermentation tank are respectively provided with an air supply interface communicated with the inside of the first fermentation tank, the second fermentation tank, the buffer fermentation tank and the third fermentation tank.
Further, the stirring shaft comprises a hollow shaft body and a first outer cladding arranged outside the shaft body, and a gap is formed between the shaft body and the first outer cladding; the stirring rod comprises a hollow rod body communicated with the inside of the shaft body and a second outer cladding layer arranged on the outer side of the rod body, a gap is reserved between the rod body and the second outer cladding layer, the first outer cladding layer and the second outer cladding layer are integrally formed, a first partition plate which is not communicated with the bottom of the first outer cladding layer is longitudinally arranged in the gap between the shaft body and the first outer cladding layer, a second partition plate which is not communicated with the end part of the second outer cladding layer, facing the inner side wall of the inner shell, of the second outer cladding layer is transversely arranged in the gap between the rod body and the second outer cladding layer, and the first partition plate is fixedly connected with the second partition plate.
Further, a water supply pipe is arranged in the shaft body, each rod body is internally provided with a water distribution hose communicated with the water supply pipe, and the water distribution hoses are connected to the cleaning device.
Further, the cleaning device comprises a water distribution main pipe arranged in the scraping plate, a water distribution branch pipe communicated with the water distribution main pipe and a plurality of check nozzles communicated with the water distribution branch pipe, wherein the water distribution main pipe is communicated with the water distribution hose, and the check nozzles are arranged on one side surface of the scraping plate facing to the inner side wall of the inner shell.
Further, the non-return nozzle includes hollow nozzle body, the orifice has been seted up to nozzle body's left end, nozzle body's right-hand member is provided with the linkage segment that can carry out shutoff with water distribution branch connection, nozzle body's inside is provided with the annular plate, the left side of annular plate is provided with the mounting panel, the lateral surface of mounting panel is provided with be connected to connecting plate on the nozzle body inside wall, the center of mounting panel is provided with the through-hole, wear to be equipped with the guide arm in the through-hole, the guide arm is provided with the end cap that can carry out shutoff to the through-hole of annular plate center department towards the one end of annular plate, cup joint elastic spring on the guide arm between end cap and the mounting panel.
Further, install the changeable annular electromagnet of polarity in the body of rod, the water distribution hose is followed pass in the through-hole of annular electromagnet, the scraper blade is provided with towards the one end of puddler and extends to inside direction connecting cylinder of puddler, direction connecting cylinder is located the one end of puddler inside is provided with magnet, through changing the polarity of annular electromagnet can pass through absorption or repulsion annular electromagnet to make the scraper blade remove towards or keep away from the puddler.
Further, the heating device comprises a heat source medium coil coiled on the outer side wall of the inner shell and a rotary connecting part arranged in the top space between the outer shell and the inner shell, and two ends of the heat source medium coil are respectively connected with a heat source medium inlet and a heat source medium outlet; the rotary connecting component is communicated with the heat source medium inlet and the heat source medium outlet through pipelines.
Further, the rotary connecting part comprises a connecting shell fixed on the inner top wall of the outer shell, a heat source medium liquid inlet cavity, a heat source medium liquid outlet cavity and a cleaning liquid buffer cavity are sequentially formed in the connecting shell from top to bottom, first connecting pipes which respectively extend into the heat source medium liquid inlet cavity and the heat source medium liquid outlet cavity are arranged on a first outer cladding of the stirring shaft, second connecting pipes which extend into the cleaning liquid buffer cavity are arranged on a water supply pipe in the shaft, the connecting shell is in rotary sealing connection with the first outer cladding, and the cleaning liquid buffer cavity is connected with a cleaning liquid supply pipe interface.
Further, a temperature sensor and a pressure sensor are arranged in the inner shell, and the temperature sensor and the pressure sensor are connected to a controller which is in communication connection with the heat source supply system, the air supply system and the microbial liquid supply system.
Advantageous effects
1. According to the utility model, the plurality of fermentation tanks which are sequentially connected through the pipelines according to the microbial fermentation process are arranged, and fermentation culture can be sequentially carried out in the different fermentation tanks according to the order of adding different strains in the microbial fermentation process and the corresponding different fermentation conditions after adding different strains, so that each fermentation culture stage is mutually independent, after the current fermentation culture is finished, bacterial liquid is conveyed to the next fermentation tank, new bacterial liquid is added, and then fermentation culture is carried out, and the next fermentation culture of bacterial liquid can be carried out by the preceding fermentation tank, so that continuous and uninterrupted fermentation culture of microorganisms is realized, and the fermentation culture efficiency of the microorganisms is effectively improved;
2. the stirring rod arranged on the periphery of the stirring shaft is provided with the scraping plate capable of moving along the length direction of the stirring rod, and the scraping plate is provided with the cleaning device, so that on one hand, the inner side wall of the inner shell of the fermentation tank can be cleaned through the cleaning device on the scraping plate, on the other hand, the liquid adhered on the inner side wall of the inner shell can be cleaned through the scraping plate, and the scraping plate can be moved to a corresponding position to correspond to the cleaning and cleaning operation on the inner side wall of the inner shell; after the bacterial liquid of the current fermentation tank is removed, cleaning and cleaning, new bacterial liquid can be added for fermentation;
3. according to the utility model, the heating device is arranged to provide a heat source for the gap between the outer shell and the inner shell, the stirring shaft and the stirring rod, so that the bacterial liquid in the inner shell is uniformly heated, and the bacterial liquid in the inner shell can be ensured to be heated more uniformly.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic diagram of a fermenter according to the present utility model;
FIG. 3 is a partial schematic view of the upper portion of FIG. 2;
FIG. 4 is an enlarged schematic view of portion B of FIG. 2;
FIG. 5 is a schematic view of the structure of the scraper according to the present utility model;
FIG. 6 is a schematic view of a check nozzle according to the present utility model;
FIG. 7 is a schematic view in section A-A of FIG. 6;
FIG. 8 is a schematic view of a stirring rod in longitudinal section according to the present utility model;
FIG. 9 is a schematic cross-sectional view of a stirring rod in accordance with the present utility model;
fig. 10 is a schematic view of the present utility model with the squeegee inclined.
In the figure: 1. a first fermenter, 101, an outer housing, 102, an inner housing, 2, a second fermenter, 3, a buffer fermenter, 4, a third fermenter, 5, a buffer tank, 6, a filter device, 7, a centrifugal concentration tank, 8, a spray drying device, 9, a driving motor, 10, a stirring shaft, 1001, a shaft body, 1002, a first outer coating, 11, a stirring rod, 1101, a rod body, 1102, a second outer coating, 1103, a second partition, 12, an air supply port, 13, a water supply pipe, 14, a water distribution hose, 15, a water distribution main, 16, a water distribution branch, 17, a check nozzle, 1701, a nozzle body, 1702, a spray orifice, 1703, connecting section, 1704, annular plate, 1705, mounting plate, 1706, connecting plate, 1707, guide rod, 1708, plug, 1709, elastic spring, 18, scraper, 19, annular electromagnet, 20, guide connecting cylinder, 21, heat source medium coil, 22, rotary connecting component, 2201, connecting shell, 2202, heat source medium liquid inlet cavity, 2203, heat source medium liquid outlet cavity, 2204, cleaning liquid buffer cavity, 2205, cleaning liquid supply pipe interface, 2206, wiring cavity, 23, heat source medium inlet, 24, heat source medium outlet, 25, temperature sensor, 26, pressure sensor.
Description of the embodiments
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
As shown in fig. 1 to 3, the present utility model provides a microorganism continuous fermentation integrated apparatus comprising a first fermenter 1, at least one second fermenter 2, a buffer fermenter 3, at least one third fermenter 4, a buffer tank 5, a filter device 6, a centrifugal concentration tank 7, and a spray drying device 8 connected by pipelines according to the process of microorganism fermentation;
in specific implementation, the number of the second fermentation tanks 2 can be selected according to the number of single strains for preparing the compound microorganisms, and the buffer fermentation tank 3 can also be used as a fermentation tank which is the same as the first fermentation tank 1 and the second fermentation tank 2 according to the situation; the bottom of the first fermentation tank 1, the second fermentation tank 2, the buffer fermentation tank 3, the third fermentation tank 4, the buffer tank 5, the centrifugal concentration tank 7 and the spray drying device 8 are all provided with discharge pipes, an electric three-way valve is arranged on the discharge pipes, one discharge port of the electric three-way valve at the bottom of the first fermentation tank 1 is connected to the upper part of the second fermentation tank 2 through a pipeline, one discharge port of the electric three-way valve at the bottom of the second fermentation tank 2 is connected to the upper part of the subsequent second fermentation tank 2 through a pipeline, one discharge port of the electric three-way valve at the bottom of the last second fermentation tank 2 is connected to the upper part of the buffer fermentation tank 3 through a pipeline, one discharge port of the electric three-way valve at the bottom of the buffer fermentation tank 3 is connected to the feed port of the electric three-way valve through a pipeline, two discharge ports of the electric three-way valve are respectively connected to the third fermentation tank 4 through a pipeline, one discharge port of the electric three-way valve at the bottom of the buffer tank 5 is connected to the filter device 6 through a pipeline, one discharge port of the electric three-way valve at the bottom of the buffer tank 5 is connected to the centrifugal concentration tank 7 through a pipeline, and the discharge port of the electric three-way valve at the bottom of the filter device is connected to the spray drying device through a filter device 7 at the bottom of the discharge port of the centrifugal concentration tank 7; the tops of the first fermentation tank 1, the second fermentation tank 2, the buffer fermentation tank 3 and the third fermentation tank 4 are respectively provided with a plurality of feed inlets, and the feed inlets are connected in corresponding single-strain storage tanks through pipelines; the electric three-way valves at the bottoms of the first fermentation tank 1, the second fermentation tank 2, the buffer fermentation tank 3, the third fermentation tank 4 and the buffer tank 5 and the centrifugal concentration tank 7 are provided with liquid pumps corresponding to pipelines of discharge ports of the next fermentation tank or concentration tank or buffer tank or legal device so as to convey bacterial liquid into the next tank;
the first fermentation tank 1, the second fermentation tank 2, the buffer fermentation tank 3 and the third fermentation tank 4 have the same structure and comprise tank bodies, the tank bodies comprise an outer shell 101 and an inner shell 102 arranged in the outer shell 101, the outer diameter of the inner shell 102 is smaller than the inner diameter of the outer shell 101, so that a gap is formed between the inner shell 102 and the side wall of the outer shell 101, the height of the inner shell 102 is smaller than the height of the outer shell 101, and the gap distance between the inner shell 102 and the upper end of the outer shell 101 is larger than the gap distance between the inner shell 102 and the lower end of the outer shell 101; the top of the outer shell 101 is provided with a driving motor 9, an output shaft of the driving motor 9 is connected with a stirring shaft 10 extending to the bottom of the inner shell 102, the whole outer body of the stirring shaft 10 is provided with a plurality of stirring rods 11, the end part of each stirring rod 11 is provided with a scraping plate 18 capable of moving along the length direction of each stirring rod 11, the scraping plates 18 are provided with a cleaning device, in the normal stirring process, the scraping plates 18 are far away from the inner side wall of the inner shell 102, at the moment, the scraping plates 18 play the role of stirring paddles like the stirring rods 11 to stir bacterial liquid, so that various bacterial liquids are uniformly mixed, after the bacterial liquid is fermented and discharged out of the tank, the inner side wall of the inner shell 102 is cleaned by the cleaning device, and then the scraping plates 18 are moved to be attached to the inner side wall of the inner shell 102 to adhere to the inner side wall of the inner shell 102;
a heating device is further disposed between the outer housing 101 and the inner housing 102, and a heat source medium provided by the heating device can flow through a gap between the outer housing 101 and the inner housing 102, the stirring shaft 10 and the stirring rod 11, so as to heat the microbial liquid in the inner housing 102.
As shown in fig. 1 and 2, the bottoms of the first fermenter 1, the second fermenter 2, the buffer fermenter 3 and the third fermenter 4 are respectively provided with an air supply interface 12 communicated with the inside of the first fermenter 1, the second fermenter 2, the buffer fermenter 3 and the third fermenter 4, and the air supply interfaces 12 are connected with an air supply system to provide air sources for the inside of the first fermenter 1, the second fermenter 2, the buffer fermenter 3 and the third fermenter 4, so as to provide air sources for microbial strain fermentation on one hand and to maintain the air pressure in the tank in a required range on the other hand.
As shown in fig. 2 to 4, 8 and 9, the stirring shaft 10 includes a hollow shaft body 1001 and a first outer cladding 1002 disposed outside the shaft body 1001, and a gap is provided between the shaft body 1001 and the first outer cladding 1002 for flowing through a heat source medium; the stirring rod 11 comprises a rod body 1101 which is hollow and is communicated with the inside of the shaft body 1001, and a second outer cladding 1102 which is arranged outside the rod body 1101, a gap is formed between the rod body 1101 and the second outer cladding 1102, the gap is also used for flowing through a heat source medium, the first outer cladding 1002 and the second outer cladding 1102 are integrally formed, a first partition plate (not shown in the drawing) which is not communicated with the bottom of the first outer cladding 1002 is longitudinally arranged in the front-rear gap between the shaft body 1001 and the first outer cladding 1002, the gap between the shaft body 1001 and the first outer cladding 1002 is divided into a left part and a right part by the first partition plate, a second partition plate 1103 which is not communicated with the end part of the second outer cladding 1102, which faces the inner side wall of the inner shell 102, is arranged in the front-rear gap between the rod body 1101 and the second outer cladding 1102 in the transverse direction, so that the gap between the rod body 1101 and the second outer cladding 1102 is divided into an upper part and a lower part by the second partition plate 1103, the first partition plate 1103 is fixedly connected with the heat source of the second partition plate, and the stirring rod 11 can flow through each stirring rod 11, so that the heat of the stirring rod 10 and the stirring rod 11 can be heated in the inner shell 102 at the same time.
As shown in fig. 2-4, a water supply pipe 13 is disposed in the shaft body 1001, each rod body 1101 is provided with a water distribution hose 14 communicated with the water supply pipe 13, the water distribution hoses 14 are connected to the cleaning device, a water supply system (such as a municipal water supply network or a pressurized water supply system formed by a water tank and a water pump) is connected to a water inlet end of the water supply pipe 13, and clean water connected to the water supply pipe 13 is supplied to the corresponding cleaning device by the water distribution hoses 14 so as to clean the side wall of the inner housing 102.
As shown in fig. 5, the cleaning device includes a main water distribution pipe 15 disposed in the scraper 18, a branch water distribution pipe 16 communicating with the main water distribution pipe 15, and a plurality of check nozzles 17 communicating with the branch water distribution pipe 16, the number of the check nozzles 17 may be set according to practical applications, for example, the number of the check nozzles 17 may be 5, 6 or 7, and the plurality of check nozzles 17 may be uniformly arranged along the length direction of the scraper 18; the main pipe 15 communicates with the main pipe 14, and the check nozzle 17 is mounted on a side of the scraper 18 facing the inner side wall of the inner housing 102, so that the fresh water supplied from the main pipe 15 is distributed to the branch pipe 16, and then is sprayed to the inner side of the inner housing 102 via the check nozzle 17.
As shown in fig. 6 and 7, the non-return nozzle 17 includes a hollow nozzle body 1701, a nozzle hole 1702 is formed at the left end of the nozzle body 1701, a connection section 1703 capable of being connected with a water distribution branch pipe 16 is provided at the right end of the nozzle body 1701, and the water distribution branch pipe 16 and the connection section 1703 can be connected by adopting a threaded connection or a welding manner; the inside of the nozzle body 1701 is provided with an annular plate 1704, the left side of the annular plate 1704 is provided with a mounting plate 1705, the outer side surface of the mounting plate 1705 is provided with a connecting plate 1706 connected to the inner side wall of the nozzle body 1701, the center of the mounting plate 1705 is provided with a through hole, a guide rod 1707 is penetrated in the through hole, one end of the guide rod 1707, facing the annular plate 1704, is provided with a plug 1708 capable of plugging the through hole in the center of the annular plate 1704, a spring 1709 is sleeved on the guide rod 1707 between the plug 1708 and the mounting plate 1705, one end of the spring 1709 is abutted against the plug 1708, and the other end is abutted against the mounting plate 1705; one end of the plug 1708 facing the annular plate 1704 is a hemispherical bulge, and the diameter of the hemispherical bulge is larger than that of a through hole in the center of the annular plate 1704, so that the hemispherical bulge can plug the through hole; under the condition that no external force is applied, under the action of the elastic force of the elastic spring 1709, the plug 1708 is in a blocking state on the through hole at the center of the annular plate 1704, after the check nozzle 17 enters water flow from the connecting section 1703, the plug 1708 is driven to move leftwards under the action of water pressure, the guide rod 1707 moves inwards in the through hole of the mounting plate 1705, so that the plug 1708 does not block the through hole at the center of the annular plate 1704 any more, water flow enters the other side of the annular plate 1704 through the through hole of the annular plate 1704, then water flow enters the other side of the mounting plate 1705 through a gap between the connecting plates 1706 at the periphery of the mounting plate 1705, and finally water flow is sprayed out through the spray holes 1702.
As shown in fig. 3, the rod 1101 is internally provided with a ring electromagnet 19 with a variable polarity, and the principle of polarity change of the ring electromagnet 19 is to change the positive and negative poles of the current flowing through the ring electromagnet to change the current direction, so as to change the polarity of the ring electromagnet 19, and how to implement the ring electromagnet 19 is specifically described in the prior art, which is not repeated herein, for example, a power line of the ring electromagnet 19 is connected to a positive and negative pole switch; the water distribution hose 14 passes through the through hole of the annular electromagnet 19, one end of the scraper 18 facing the stirring rod 11 is provided with a guide connecting cylinder 20 extending into the stirring rod 11, one end of the guide connecting cylinder 20 positioned in the stirring rod 11 is provided with a magnet, the polarity of the magnet is fixed, and the polarity of the annular electromagnet 19 is changed, so that the scraper 18 can move towards or away from the stirring rod 11 by adsorbing or repelling the annular electromagnet;
in this embodiment, in order to avoid damage to the magnet and the ring electromagnet 19 or the magnet and the stirring rod 11 caused by frequent collision during the movement of the scraper 18, a rubber gasket is disposed on a side surface of the ring electromagnet 19 facing the magnet and a side surface of the stirring rod 11 facing the magnet; in order to facilitate the installation of annular electromagnet 19, a mounting seat is provided on the inner side wall of rod body 1101, and annular electromagnet 19 is fixedly installed on the mounting seat.
As shown in fig. 2 and 3, the heating device comprises a heat source medium coil 21 coiled on the outer side wall of the inner housing 102, and a rotary connecting member 22 arranged in the top space between the outer housing 101 and the inner housing 102, wherein two ends of the heat source medium coil 21 are respectively connected with a heat source medium inlet 23 and a heat source medium outlet 24; the rotary connecting part 22 is communicated with the heat source medium inlet 23 and the heat source medium outlet 24 through pipelines;
the rotary connection component 22 comprises a connection shell 2201 fixed on the inner top wall of the outer shell 101, a heat source medium liquid inlet cavity 2202, a heat source medium liquid outlet cavity 2203 and a cleaning liquid buffer cavity 2204 are sequentially arranged in the connection shell 2201 from top to bottom, a first connection pipe 1003 extending into the heat source medium liquid inlet cavity 2202 and the heat source medium liquid outlet cavity 2203 respectively is arranged on a first outer cladding 1002 of the stirring shaft 10, a second connection pipe 1301 extending into the cleaning liquid buffer cavity 2204 is arranged on a water supply pipe 13 in the shaft body 1001, the connection shell 2201 is in rotary sealing connection with the first outer cladding 1002, and a cleaning liquid supply pipe interface 2205 is connected with the cleaning liquid buffer cavity 2204; the heat source medium inlet 23 is communicated with the heat source medium liquid inlet 2202 through a pipeline, the heat source medium outlet 24 is communicated with the heat source medium liquid outlet 2203 through a pipeline, the corresponding connecting shell 2201 of the cleaning liquid buffer cavity 2204 is connected with a water inlet port extending out of the outer side surface of the outer shell 101, and the water inlet port is connected with a water supply system; in order to facilitate the power supply to the annular electromagnet 19, a wiring cavity 2206 is further arranged at the lower part of the connecting shell 2201, a slip ring is arranged in the wiring cavity 2206, and all connecting wires of the annular electromagnet 19 are connected with the positive-negative pole change-over switch through the slip ring.
As shown in fig. 2 and 3, a temperature sensor 25 and a pressure sensor 26 are disposed in the inner housing 102, the temperature sensor 25 and the pressure sensor 26 are connected to a controller, the controller is communicatively connected to a heat source supply system, a gas supply system and a microbial liquid supply system, the controller is connected to an upper computer, and the upper computer inputs operation parameters of the continuous microbial fermentation integrated device.
In the embodiment of the utility model, at least two rows of stirring rods 11 are arranged along the circumferential direction of the stirring shaft 10, each row of stirring rods 11 is located on the same vertical plane, the scraping plates 18 are vertically arranged, the ends of two upper and lower adjacent scraping plates 18 are in contact with each other, or the scraping plates 18 are obliquely arranged (as shown in fig. 10), when the scraping plates 18 are obliquely arranged, the side surface of the scraping plates 18 facing the inner shell 102 is an arc surface matched with the inner arc surface of the inner shell 102, and the lower end of the scraping plate 18 located on the upper side of the two upper and lower adjacent scraping plates 18 is lower than the upper end of the scraping plate 18 located on the lower side, so that the scraping plates 18 can clean the liquid adhered on the inner shell 102 in the rotation process of the stirring shaft 10.
The following description is made on the working process of the continuous fermentation integrated device of the microorganism provided by the utility model by taking three single bacterial liquids as an example (see the bacterial types and the fermentation process disclosed in the Chinese patent application with the application publication number of CN 104371960A) for compounding the compound microorganism:
in this case, the number of second fermenter 2 is one, and the scrapers 18 in all fermenter are pressed against the end of the stirring rod 11 by the attraction of the ring electromagnet 19 and the magnet; firstly adding basic fermentation liquor into a first fermentation tank 1, inputting working parameters of the first fermentation tank 1, a second fermentation tank 2, a buffer fermentation tank 3 and a third fermentation tank 4 through an upper computer, controlling a heating device of a heat source supply system to provide heat source media for the first fermentation tank 1, the second fermentation tank 2, the buffer fermentation tank 3 and the third fermentation tank 4, controlling an air supply system to provide air sources for the first fermentation tank 1, the second fermentation tank 2, the buffer fermentation tank 3 and the third fermentation tank 4, actually detecting the temperature and the pressure in the corresponding fermentation tanks through a temperature sensor 25 and a pressure sensor 26, feeding back the detected temperature and pressure values to the controller, and sending a command for adjusting the heat source temperature to the heat source supply system after the controller compares the received temperature detection values with the set working temperature so as to ensure that the temperature in the first fermentation tank 1, the second fermentation tank 2, the buffer fermentation tank 3 and the third fermentation tank 4 is maintained within a preset temperature range; then adding a first single bacterial liquid into the first fermentation tank 1, controlling a driving motor 9 of the first fermentation tank 1 to operate by a controller, driving a stirring shaft 10 to rotate in the operation process of the driving motor 9, and driving a stirring rod 11 to rotate by the stirring shaft 10 to stir the fermentation liquid in the first fermentation tank 1, so that the fermentation liquid and the first single bacterial liquid are fully mixed on one hand, and the fermentation liquid is uniformly heated on the other hand; after fermentation of the fermentation liquor in the first fermentation tank 1 is completed, the controller controls an electric three-way valve and a liquid pump on the first fermentation tank 1 to be opened, and closes a driving motor 9 on the first fermentation tank 1 to convey the fermentation liquor in the first fermentation tank 1 to the second fermentation tank 2, then a second single bacterial liquid is added to the second fermentation tank 2, then the controller controls the driving motor of the second fermentation tank 2 to be started to stir the fermentation liquor in the second fermentation tank 2, after the second fermentation tank 2 is fermented to a preset time, the electric three-way valve and the liquid pump corresponding to the second fermentation tank 2 are started to convey the fermentation liquor in the second fermentation tank 2 to a buffer fermentation tank 3 for static fermentation, after the fermentation liquor in the buffer fermentation tank 3 is completed for a preset fermentation time, the electric three-way valve and the liquid pump corresponding to the buffer fermentation tank 3 are started to convey the fermentation liquor in the buffer fermentation tank 3 to one third fermentation tank 4, then a third single bacterial liquid is added to the third fermentation tank 4, after the fermentation liquor in the third fermentation tank 4 is kept still to be controlled to be set to a preset time, and the three-way valve and the liquid pump is sequentially sprayed to a drying device for drying and a final fermentation tank is sprayed to be subjected to a filter and a final fermentation liquor is obtained after the fermentation liquor is subjected to a drying device of the filtration and is subjected to a filtration device of the filtration;
after the first fermentation tank 1 conveys the fermentation liquor in the first fermentation tank to the second fermentation tank 2, the controller closes a liquid pump and a gas supply system corresponding to the first fermentation tank 1, and switches an electric three-way valve corresponding to the first fermentation tank 1 to another passage, then the controller controls and controls the water supply system to work so as to provide cleaning liquid in the first fermentation tank 1, the cleaning liquid is sprayed onto the inner side wall of the inner shell 102 by a check nozzle 17 on the scraper 18, in order to ensure the cleaning effect, the controller synchronously starts a driving motor of the first fermentation tank 1, so that the scraper 18 rotates in the first fermentation tank 1, after the water supply system works for a preset time, the controller stops the operation of the water supply system, and simultaneously controls an anode-cathode switch to change the current flow direction of the annular electromagnet 19, so as to change the polarity of the annular electromagnet 19, so that the annular electromagnet 19 and the magnet at the moment repel each other due to homopolarity, the scraper is attached to the inner side wall of the inner shell 102 of the first fermentation tank 1, the scraper 18 scrapes the liquid adhered on the inner wall of the inner shell 102 of the first fermentation tank 1 under the driving of the driving motor 9, and the cleaning liquid is cleaned in the same way after the first fermentation tank 1 is cleaned by the same three-way, and the same fermentation tank is cleaned after the same fermentation tank is cleaned; the number of the third fermentation tanks 4 is two in the utility model, because the fermentation time in the link is longer than the working time of the preceding fermentation tanks, so that the whole continuous fermentation process is not influenced, the two third fermentation tanks 4 can be alternately used in different fermentation processes, each fermentation tank is recycled, and each subsequent fermentation work is not influenced, thereby realizing continuous fermentation.
The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.
Claims (10)
1. The microorganism continuous fermentation integrated device is characterized by comprising a first fermentation tank (1), at least one second fermentation tank (2), a buffer fermentation tank (3), at least one third fermentation tank (4), a buffer tank (5), a filtering device (6), a centrifugal concentration tank (7) and a spray drying device (8) which are connected in sequence through pipelines according to the microorganism fermentation process;
the structure of the first fermentation tank (1), the structure of the second fermentation tank (2), the structure of the buffer fermentation tank (3) and the structure of the third fermentation tank (4) are the same, the structure of the buffer fermentation tank comprises tank bodies, each tank body comprises an outer shell (101) and an inner shell (102) arranged in the outer shell (101), a driving motor (9) is arranged at the top of the outer shell (101), a stirring shaft (10) extending to the bottom of the inner shell (102) is connected with an output shaft of the driving motor (9), a plurality of stirring rods (11) are arranged on the whole body of the stirring shaft (10), scraping plates (18) capable of moving along the length direction of the stirring rods (11) are arranged at the end parts of the stirring rods (11), and cleaning devices are arranged on the scraping plates (18);
and a heating device is further arranged between the outer shell (101) and the inner shell (102), and a heat source medium provided by the heating device can flow through a gap between the outer shell (101) and the inner shell (102), the stirring shaft (10) and the stirring rod (11) so as to heat microbial bacteria liquid in the inner shell (102).
2. The microorganism continuous fermentation integrated apparatus according to claim 1, wherein: the bottom of the first fermentation tank (1), the second fermentation tank (2), the buffer fermentation tank (3) and the third fermentation tank (4) are respectively provided with an air supply interface (12) communicated with the inside of the first fermentation tank, the second fermentation tank and the third fermentation tank.
3. The microorganism continuous fermentation integrated apparatus according to claim 1, wherein: the stirring shaft (10) comprises a hollow shaft body (1001) and a first outer cladding (1002) arranged outside the shaft body (1001), wherein a gap is formed between the shaft body (1001) and the first outer cladding (1002); puddler (11) include cavity, and with body of rod (1101) and setting of the inside intercommunication of axis body (1001) are in body of rod (1101) outside second outer covering (1102), have the clearance between body of rod (1101) and second outer covering (1102), first outer covering (1002) and second outer covering (1102) integrated into one piece, clearance around between axis body (1001) and first outer covering (1002) is provided with first baffle that does not communicate with first outer covering (1002) bottom along vertically, clearance around between body of rod (1101) and second outer covering (1102) is provided with second baffle (1103) that does not communicate with second outer covering (1102) towards the tip of inner casing (102) inside wall along transversely, first baffle and second baffle (1103) fixed connection.
4. A microorganism continuous fermentation integrated apparatus according to claim 3, wherein: a water supply pipe (13) is arranged in the shaft body (1001), a water distribution hose (14) communicated with the water supply pipe (13) is arranged in each rod body (1101), and the water distribution hose (14) is connected to the cleaning device.
5. The microorganism continuous fermentation integrated apparatus according to claim 4, wherein: the cleaning device comprises a water distribution main pipe (15) arranged in the scraping plate (18), a water distribution branch pipe (16) communicated with the water distribution main pipe (15) and a plurality of check nozzles (17) communicated with the water distribution branch pipe (16), wherein the water distribution main pipe (15) is communicated with the water distribution hose (14), and the check nozzles (17) are arranged on one side surface of the scraping plate (18) facing the inner side wall of the inner shell (102).
6. The microorganism continuous fermentation integrated apparatus according to claim 5, wherein: the check nozzle (17) comprises a hollow nozzle body (1701), a spray hole (1702) is formed in the left end of the nozzle body (1701), a connecting section (1703) capable of being connected with a water distribution branch pipe (16) is arranged at the right end of the nozzle body (1701), an annular plate (1704) is arranged in the nozzle body (1701), a mounting plate (1705) is arranged on the left side of the annular plate (1704), a connecting plate (1706) connected to the inner side wall of the nozzle body (1701) is arranged on the outer side face of the mounting plate (1705), a through hole is formed in the center of the mounting plate (1705), a guide rod (1707) is arranged in the through hole in a penetrating mode, a plug (8) capable of plugging the through hole in the center of the annular plate (1704) is arranged at one end of the guide rod (1707), and an elastic spring (1709) is sleeved on the guide rod (1707) between the plug (1708) and the mounting plate (1705).
7. The microorganism continuous fermentation integrated apparatus according to claim 4, wherein: install changeable annular electromagnet (19) of polarity in body of rod (1101), water distribution hose (14) follow pass in the through-hole of annular electromagnet (19), scraper blade (18) are provided with towards the one end of puddler (11) and extend to inside direction connecting cylinder (20) of puddler (11), direction connecting cylinder (20) are located the one end of puddler (11) inside is provided with magnet, through changing the polarity of annular electromagnet (19), can pass through absorption or repulsion annular electromagnet to make scraper blade (18) remove towards or keep away from puddler (11).
8. A microorganism continuous fermentation integrated apparatus according to claim 3, wherein: the heating device comprises a heat source medium coil pipe (21) coiled on the outer side wall of the inner shell (102) and a rotary connecting part (22) arranged in a top space between the outer shell (101) and the inner shell (102), and two ends of the heat source medium coil pipe (21) are respectively connected with a heat source medium inlet (23) and a heat source medium outlet (24); the rotary connecting part (22) is communicated with the heat source medium inlet (23) and the heat source medium outlet (24) through pipelines.
9. The microorganism continuous fermentation integrated apparatus according to claim 8, wherein: the rotary connecting component (22) comprises a connecting shell (2201) fixed on the inner top wall of the outer shell (101), a heat source medium liquid inlet cavity (2202), a heat source medium liquid outlet cavity (2203) and a cleaning liquid buffer cavity (2204) are sequentially arranged in the connecting shell (2201) from top to bottom, first connecting pipes (1003) respectively extending to the heat source medium liquid inlet cavity (2202) and the heat source medium liquid outlet cavity (2203) are arranged on a first outer cladding (1002) of the stirring shaft (10), a second connecting pipe (1301) extending to the cleaning liquid buffer cavity (2204) is arranged on a water supply pipe (13) in the shaft body (1001), the connecting shell (2201) is in rotary sealing connection with the first outer cladding (1002), and the cleaning liquid buffer cavity (2204) is connected with a cleaning liquid supply pipe interface (2205).
10. The microorganism continuous fermentation integrated apparatus according to any one of claims 1 to 9, wherein: the inner shell (102) is internally provided with a temperature sensor (25) and a pressure sensor (26), the temperature sensor (25) and the pressure sensor (26) are connected to a controller, and the controller is in communication connection with a heat source supply system, a gas supply system and a microbial liquid supply system.
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CN202311158230.6A CN116970485A (en) | 2023-09-08 | 2023-09-08 | Microorganism continuous fermentation integrated device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN117363480A (en) * | 2023-12-05 | 2024-01-09 | 上海乐纯生物技术股份有限公司 | Biological reactor |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN117363480A (en) * | 2023-12-05 | 2024-01-09 | 上海乐纯生物技术股份有限公司 | Biological reactor |
CN117363480B (en) * | 2023-12-05 | 2024-02-20 | 上海乐纯生物技术股份有限公司 | Biological reactor |
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