CN115975774A - Anti-pollution formula bacteria culture device of microbiological assay - Google Patents
Anti-pollution formula bacteria culture device of microbiological assay Download PDFInfo
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- CN115975774A CN115975774A CN202211719349.1A CN202211719349A CN115975774A CN 115975774 A CN115975774 A CN 115975774A CN 202211719349 A CN202211719349 A CN 202211719349A CN 115975774 A CN115975774 A CN 115975774A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 105
- 230000002906 microbiologic effect Effects 0.000 title claims abstract description 9
- 238000003556 assay Methods 0.000 title description 2
- 230000005540 biological transmission Effects 0.000 claims abstract description 37
- 230000017105 transposition Effects 0.000 claims abstract description 31
- 238000010438 heat treatment Methods 0.000 claims abstract description 29
- 238000012423 maintenance Methods 0.000 claims abstract description 28
- 230000007246 mechanism Effects 0.000 claims abstract description 27
- 238000002474 experimental method Methods 0.000 claims abstract description 18
- 230000000813 microbial effect Effects 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 230000001954 sterilising effect Effects 0.000 claims description 33
- 239000000243 solution Substances 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 27
- 238000003756 stirring Methods 0.000 claims description 24
- 238000003860 storage Methods 0.000 claims description 23
- 230000002093 peripheral effect Effects 0.000 claims description 20
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 19
- 229910052760 oxygen Inorganic materials 0.000 claims description 19
- 239000001301 oxygen Substances 0.000 claims description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- 239000011521 glass Substances 0.000 claims description 12
- 239000002699 waste material Substances 0.000 claims description 12
- 238000005070 sampling Methods 0.000 claims description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 238000002347 injection Methods 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- 230000001681 protective effect Effects 0.000 claims description 10
- 238000009826 distribution Methods 0.000 claims description 8
- 239000011248 coating agent Substances 0.000 claims description 7
- 238000000576 coating method Methods 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 5
- 230000001502 supplementing effect Effects 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 22
- 230000009286 beneficial effect Effects 0.000 abstract description 8
- 239000012530 fluid Substances 0.000 abstract description 3
- 238000001802 infusion Methods 0.000 abstract description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 45
- 235000017491 Bambusa tulda Nutrition 0.000 description 45
- 241001330002 Bambuseae Species 0.000 description 45
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 45
- 239000011425 bamboo Substances 0.000 description 45
- 238000000034 method Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 230000008569 process Effects 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 6
- 239000002912 waste gas Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
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- 230000000149 penetrating effect Effects 0.000 description 2
- 230000003405 preventing effect Effects 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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Abstract
The invention relates to the technical field of bacterial culture, in particular to a microbial experiment anti-pollution type bacterial culture device. Anti-pollution formula bacteria culture device of microbiological experiment, including the frame, the inner wall of frame rotates to be connected with rotates the frame through the rotating electrical machines driven transposition, and the transmission assembly is installed to the side of week of frame is revolved in the transposition, and mutually isolated fluid infusion chamber, exhaust chamber, air inlet chamber and heating chamber have been seted up from top to bottom in proper order to the inside of frame is revolved in the transposition, and the top fixed mounting that the frame was revolved in the transposition has quick-witted case, and the internally mounted of machine case has supplementary module. The invention has the beneficial effects that: according to the invention, through the arrangement of the culture mechanism, the transposition rotating frame and other structures, the device can highly absorb bacteria to complete the culture operation of the bacteria, and the device can realize the automatic maintenance of the bacteria culture environment during the bacteria culture, so that the automation efficiency and the automation degree of the device during the bacteria culture are effectively improved.
Description
Technical Field
The invention relates to the technical field of bacterial culture, in particular to an anti-pollution bacterial culture device for a microbial experiment.
Background
Bacterial culture is a technique in which bacteria are grown and propagated artificially. The bacteria are widely distributed in nature, large in quantity and multiple in types, can benefit mankind, and can also become a cause of diseases. Most bacteria can be cultured artificially by inoculating them on a culture medium and allowing them to grow and propagate. The cultured bacteria are used for research, identification and application. Bacterial culture is a complex technique. In the prior art, in biological experiments, the culture of bacteria is usually carried out in a bacteria incubator, but in the process of adding the medicament, the bacteria is inevitably contacted with air or interfered by other bacteria, so that the bacterial culture result is influenced, and therefore, the improvement space exists.
In the prior art, a patent document with publication number CN106867895a discloses a microbial experiment anti-pollution type bacterial culture device, which adds a medicine into a culture dish in the middle of a rubber tube through an injector, drives a rack rod through a motor after the medicine is added, enables a stop push rod to extrude the rubber tube onto a stop base so as to be closed, and then performs vacuum pumping through a vacuum pump so as to prevent pollution in an incubator, is convenient to operate, ensures the accuracy of a culture result, and has popularization and use values.
Disclosure of Invention
Aiming at the technical problems in the prior art, the invention provides a microbial experiment anti-pollution type bacteria culture device to solve the problems that the prior scheme can not automatically maintain the bacteria culture environment during bacteria culture, the bacteria are easy to stick to the wall during culture, and the prior bacteria culture device can not timely perform ultraviolet sterilization on the inner wall of a culture mechanism before and after culture during culture, so that the anti-pollution effect is limited.
The technical scheme for solving the technical problems is as follows: anti-pollution formula bacteria cultivation device of microbiological experiment, the test platform comprises a support, the inner wall of frame rotates and is connected with and revolves the frame through the rotating electrical machines driven transposition, transmission assembly is installed to the side all around of transposition revolves the frame, mutually isolated's moisturizing chamber, exhaust chamber, air inlet chamber and heating chamber have been seted up in proper order from top to bottom to the inside of transposition revolves the frame, the top fixed mounting that the transposition revolves the frame has quick-witted case, the internally mounted of machine case has supplementary module, the top of moisturizing chamber, exhaust chamber, air inlet chamber and heating chamber all communicates through pipeline and supplementary module, the side all fixed mounting that the transposition revolves the frame has a set of cultivation mechanism that is the circumference array and distributes, and is a set of cultivate the mechanism and all drive, every through transmission assembly the port of cultivateing the mechanism all communicates with moisturizing chamber, exhaust chamber, air inlet chamber and heating chamber.
The invention has the beneficial effects that:
1) According to the invention, through the arrangement of the culture mechanism, the transposition rotating frame and other structures, the device can highly absorb bacteria to complete the culture operation of the bacteria, and the device can realize the automatic maintenance of the bacteria culture environment during the bacteria culture, so that the automation efficiency and the automation degree of the device during the bacteria culture are effectively improved.
2) When the oxygen injection operation is carried out, the ultraviolet lamp ring is kept open, the ultraviolet sterilizing lamp is opened before the bacteria culture solution and bacteria are added, the ultraviolet sterilizing lamp is opened after the ultraviolet sterilizing lamp is opened, the ultraviolet sterilizing operation is carried out on the inner walls of the maintenance cylinder and the cultivation cylinder, the probability that the bacteria and the bacteria culture are polluted after the bacteria and the bacteria are added is effectively reduced through the realization of the ultraviolet pre-sterilizing effect, the ultraviolet sterilizing lamp is kept closed during the bacteria adding and normal cultivation operation, after the bacteria and the bacteria liquid in the cultivation mechanism are discharged, the ultraviolet sterilizing lamp is opened, the secondary sterilization of the inner walls of the cultivation mechanism is carried out, and the wall adhesion preventing effect of the bacteria and the bacteria culture solution on the inner walls of the cultivation mechanism is effectively improved through the setting of the anti-adhesion coating and the rotation state of the cultivation cylinder.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, cultivate the mechanism and respectively including maintaining a section of thick bamboo and installing in the inside singlechip of frame, the interior top fixed mounting of maintaining a section of thick bamboo has temperature humidity probe, the week side fixed mounting of maintaining a section of thick bamboo has the vibration exciter, the fixed intercommunication in top of maintaining a section of thick bamboo has into the appearance pipe, the fixed intercommunication in week side of going into the appearance pipe has the culture solution notes pipe, the other end and the fixed intercommunication in fluid infusion chamber of culture solution notes pipe, the week side of maintaining a section of thick bamboo is fixed intercommunication respectively has exhaust branch and air inlet branch, exhaust branch's the other end and the fixed intercommunication in exhaust chamber, air inlet branch's the other end and the fixed intercommunication in air inlet chamber, the sterilization subassembly is installed to the axis position of maintaining a section of thick bamboo, the bottom of maintaining a section of thick bamboo is rotated the intercommunication and has a cultivation section of thick bamboo, the inside collocation of cultivation section of thick bamboo has the water bath subassembly, the bottom of cultivation section of thick bamboo is rotated and is connected with a kind shaft section of thick bamboo, a kind shaft section of thick bamboo and a cultivation section of thick bamboo are all driven through the transmission assembly, a set of thick bamboo top that goes out the top of thick bamboo sets up a set of leakage hole that is the circumference array distributes is seted up, the top fixed mounting that goes out the stirring axle is the stirring shaft side of thick bamboo and is fixed side of stirring is installed a set of stirring the side and is connected with the dislocation discharge shelf, the circulation frame of sample discharge frame, the rotation of sample discharge frame is connected with the rotation.
The beneficial effects of adopting the above-mentioned further scheme are that, during the use, the effect that the singlechip sets up lies in carrying out well accuse operation to corresponding electric mechanism in this device, wait that the inside of the bacterial culture of cultivateing section of thick bamboo is added by going into appearance pipe department, after the bacterial culture adds, to the inside customization nutrient solution of cultivateing the section of thick bamboo, during the bacterial culture operation, humiture probe carries out real-time supervision to the humiture data of maintaining a section of thick bamboo inside, and humiture probe during operation, with real-time humiture data feedback to the singlechip of monitoring, the singlechip is according to the data feedback of humiture probe with the water bath temperature of control water bath subassembly, when the bacterium cultivates the operation, exhaust branch pipe is used for regularly discharging the waste gas that produces in the inside bacterial culture process of maintaining a section of thick bamboo, air inlet branch pipe then carries out the supply operation of oxygen in the maintaining section of thick bamboo, then carry out the operation of cultivateing of bacterium, the effect that the culture liquid notes pipe set up lies in carrying out the replenishment operation of bacterial culture liquid to carrying out in the maintenance section of thick bamboo, then for the normal operation of bacteria, when the bacterial culture liquid advances in the vibration exciter, then the state work of the vibration exciter and the synchronous operation of stirring, then carry out the rigid stirring and the bacterial culture liquid of the bacteria, the strain culture, further, the even growth of the stirring, thereby the bacterium culture shaft is produced, the even when the culture medium culture is opened, the culture is in the strain culture medium, the vibration of the culture is in the process, thereby, the strain culture medium culture shaft, the strain culture is even, the strain culture is opened, the strain culture is in the process, thereby, the strain culture is produced, the strain culture shaft, the strain culture medium is in the vibration frequency, thereby, the strain culture medium is opened, the strain culture is more.
Further, the transmission assembly is connected in the internal rotation section of thick bamboo in the transposition revolves the frame outside including being fixed in the transposition respectively, rotating, the week side of internal rotation section of thick bamboo rotates and is connected with the external rotation section of thick bamboo, the equal fixed mounting in all sides of internal rotation section of thick bamboo and external rotation section of thick bamboo has transmission ring gear, the equal fixed mounting in both sides face of support has drive machine, two drive machine's output axle head is connected with the transmission of internal rotation section of thick bamboo and external rotation section of thick bamboo respectively, two the week side of transmission ring gear is connected with a kind axle section of thick bamboo and a transmission of cultivating a kind respectively with a kind axle section of thick bamboo.
Furthermore, driven bevel gear rings are fixedly mounted on the peripheral side surfaces of the inner rotary cylinder and the outer rotary cylinder, and driving bevel gears meshed with the driven bevel gear rings are fixedly mounted at the output shaft ends of the two transmission motors.
Furthermore, the all fixed mounting in the week side of going out a kind beam barrel and a cultivation section of thick bamboo has driven ring gear, two the week side of driven ring gear meshes with two transmission ring gears respectively, it is the closed bottom end open-ended cavity tubular structure in top to go out a kind beam barrel, the week side fixed intercommunication of pilot tube has the sampling tube.
Adopt above-mentioned further scheme's beneficial effect is, the during operation, two driving motor are independent operating condition, two driving motor equal independent work promptly, when needs make stir axle output rotational speed and stir the operation, go out a appearance beam barrel and be driven with the settlement state, then make to stir the axle with settlement state output rotational speed, at the in-process that the bacterium normally cultivateed, cultivate to be managed by periodic slow rotating, through cultivating the rotating state setting of a section of thick bamboo, thereby improve bacterium and bacterium culture and cultivate the antiseized wall effect of a section of thick bamboo inner wall.
Furthermore, the interior of the sample discharge pipe, the sampling pipe, the sample inlet pipe, the culture solution injection pipe, the exhaust branch pipe and the air inlet branch pipe are all provided with electromagnetic valves.
The beneficial effect of adopting above-mentioned further scheme is that, during operation, above-mentioned solenoid valve is controlled by the singlechip and is opened, through the setting of solenoid valve to control the worker's form state of arranging appearance pipe, sampling tube, income appearance pipe, culture solution notes pipe, exhaust branch and inlet branch.
Further, the sterilization assembly comprises a glass protective tube arranged at the axis position of the maintenance tube, an ultraviolet sterilization lamp is fixedly arranged at the axis position of the glass protective tube, and an anti-sticking coating is fixedly arranged on the outer wall of the glass protective tube.
Adopt above-mentioned further scheme's beneficial effect is, before bacteria culture solution and bacterium add, the ultraviolet sterilizing lamp is opened, the back is opened to the ultraviolet sterilizing lamp, then carry out the ultraviolet sterilization operation to the inner wall of a maintenance section of thick bamboo and a cultivation section of thick bamboo, the realization through ultraviolet sterilization effect in advance, thereby effectively reduce bacterium and bacterium culture contaminated probability after adding, the bacterium adds and when the operation is normally cultivated to the bacterium, the ultraviolet sterilizing lamp keeps closing, after bacterium and the bacterium liquid discharge in cultivateing the mechanism finishes, the ultraviolet sterilizing lamp is opened, then cultivate the secondary sterilization of mechanism inner wall, setting through anti-sticking coating, thereby effectively improve bacterium and bacteria culture solution at the antiseized wall effect on glass pillar surface.
Further, the water bath subassembly is including offering the water bath annular chamber of a section of thick bamboo inside of cultivating up, with cultivate to cultivate a section of thick bamboo and rotate the cloth liquid ring canal of being connected and be fixed in the heating stick that heats the intracavity portion, cultivate to cultivate the inside of a section of thick bamboo and offer a set of liquid hole that is circumference array distribution, cloth liquid ring canal and water bath annular chamber pass through liquid hole intercommunication, the fixed intercommunication in top surface of water bath annular chamber has the water bath tubulation, the other end and the fixed intercommunication in heating chamber of water bath tubulation.
Adopt above-mentioned further scheme's beneficial effect is, when carrying out the normal cultivation operation of bacterium, the inside of water bath ring chamber and heating chamber all is mended the hot water of sufficient bath, and when carrying out the water bath operation, the heating rod works with setting for the state, and the heating rod work back heats the operation to the hot water of bath then, and during the heating, the heating rod makes the inside temperature of cultivating the section of thick bamboo maintain for the settlement temperature, then realizes cultivating the constant temperature of bacterium in the section of thick bamboo.
Further, supplementary module includes respectively with quick-witted case fixed connection's oxygen storage tank, storage waste tank, inlet tube, aspiration pump, the one end and the fixed intercommunication in exhaust chamber of aspiration pump air inlet, the one end and the fixed intercommunication in storage waste tank of aspiration pump gas outlet, the one end and the fixed intercommunication in chamber of admitting air of oxygen storage tank gas outlet, the end of oxygen storage tank, storage waste tank and intake pipe all communicates there is the joint, every the tip that connects all extends to the outside of quick-witted case.
Further, the inside of air inlet chamber from top to bottom fixed mounting has ultraviolet lamp ring and active carbon filter core in proper order.
Adopt the beneficial effect of above-mentioned further scheme to be, when the injection operation of carrying out oxygen, the ultraviolet lamp ring keeps opening, through the setting of ultraviolet lamp ring and active carbon filter core to aseptic effect when guaranteeing to advance oxygen, the contaminated rate when further reducing the bacteria cultivation then.
Drawings
FIG. 1 is a schematic view of the overall structure of the contamination-proof bacteria culture container for the microorganism experiment of the present invention;
FIG. 2 is a schematic cross-sectional view of FIG. 1 according to the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2A;
FIG. 4 is an enlarged view of a portion of FIG. 2 at B according to the present invention;
FIG. 5 is an enlarged view of a portion of the structure of FIG. 2 at C;
FIG. 6 is a schematic sectional view of a culture mechanism according to the present invention;
fig. 7 is a partial enlarged view of fig. 6 at D according to the present invention.
In the drawings, the reference numbers indicate the following list of parts:
1. a machine base; 2. a rotating electric machine; 3. rotating the rotary frame; 4. a fluid infusion cavity; 5. an exhaust chamber; 6. an air inlet cavity; 7. a heating chamber; 8. a chassis; 9. an auxiliary module; 10. a culture mechanism; 11. maintaining the cylinder; 12. a temperature and humidity probe; 13. a vibration exciter; 14. a sample inlet pipe; 15. injecting a culture solution; 16. an exhaust branch pipe; 17. an intake branch pipe; 18. a cultivation cylinder; 19. a sample outlet shaft cylinder; 20. stirring blades; 21. a sample discharge pipe; 22. an internal rotation cylinder; 23. an outward rotating cylinder; 24. a transmission gear ring; 25. a drive motor; 26. a driven gear ring; 27. a sampling tube; 28. an electromagnetic valve; 29. a glass protective tube; 30. an ultraviolet sterilizing lamp; 31. water bath annular cavity; 32. a liquid distribution ring pipe; 33. a heating rod; 34. water bath connecting pipes; 35. an ultraviolet lamp ring; 36. an activated carbon filter element.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth to illustrate, but are not to be construed to limit the scope of the invention.
The present invention provides the following preferred embodiments
As shown in fig. 1-7, the microorganism experiment anti-pollution type bacteria culture device comprises a machine base 1, wherein the inner wall of the machine base 1 is rotationally connected with a transposition rotating frame 3 driven by a rotating motor 2, the surface of the motor is fixedly connected with the machine base 1, and the output shaft end of the motor is fixedly connected with the transposition rotating frame 3;
the peripheral side surface of the transposition rotating frame 3 is provided with a transmission component, a liquid supplementing cavity 4, an exhaust cavity 5, an air inlet cavity 6 and a heating cavity 7 which are mutually isolated are sequentially arranged in the transposition rotating frame 3 from top to bottom, the top end of the transposition rotating frame 3 is fixedly provided with a case 8, an auxiliary module 9 is arranged in the case 8, and the top ends of the liquid supplementing cavity 4, the exhaust cavity 5, the air inlet cavity 6 and the heating cavity 7 are communicated with the auxiliary module 9 through pipelines;
an ultraviolet lamp ring 35 and an activated carbon filter element 36 are fixedly installed inside the air inlet cavity 6 from top to bottom in sequence, when oxygen is injected, the ultraviolet lamp ring 35 is kept opened, and the ultraviolet lamp ring 35 and the activated carbon filter element 36 are arranged, so that the sterile effect during oxygen inlet is ensured, and the pollution rate during bacterial culture is further reduced;
the auxiliary module 9 comprises an oxygen storage tank, a waste storage tank, a water inlet pipe and an air pump which are fixedly connected with the chassis 8 respectively, the oxygen storage tank is used for storing high-pressure pure oxygen, and the waste storage tank is used for storing waste gas in the process of culturing bacteria;
one end of an air inlet of the air pump is fixedly communicated with the exhaust cavity 5, one end of an air outlet of the air pump is fixedly communicated with the waste storage tank, and the air pump is used for fully exhausting waste gas generated during bacterial culture;
one end of the air outlet of the oxygen storage tank is fixedly communicated with the air inlet cavity 6, the ends of the oxygen storage tank, the waste storage tank and the air inlet pipe are communicated with joints, the end part of each joint extends to the outside of the case 8, the joints are arranged to supplement water for the water inlet pipe and pressurize and add oxygen for the oxygen storage tank, and the joints at the waste storage tank are arranged to discharge waste gas stored in the waste storage tank;
a group of culture mechanisms 10 distributed in a circumferential array are fixedly installed on the peripheral side surface of the transposition rotary frame 3, the culture mechanisms 10 are all driven by a transmission assembly, and the port of each culture mechanism 10 is communicated with the liquid supplementing cavity 4, the air exhaust cavity 5, the air inlet cavity 6 and the heating cavity 7.
The cultivation mechanism 10 comprises a maintenance cylinder 11 and a single chip microcomputer installed in the base 1 respectively, a temperature and humidity probe 12 is fixedly installed at the inner top of the maintenance cylinder 11, a vibration exciter 13 is fixedly installed on the peripheral side face of the maintenance cylinder 11, and the type of the temperature and humidity probe 12 can be HR-NTC;
a sample inlet pipe 14 is fixedly communicated with the top end of the maintenance cylinder 11, a culture solution injection pipe 15 is fixedly communicated with the peripheral side surface of the sample inlet pipe 14, the other end of the culture solution injection pipe 15 is fixedly communicated with the solution supplementing cavity 4, an exhaust branch pipe 16 and an air inlet branch pipe 17 are respectively fixedly communicated with the peripheral side surface of the maintenance cylinder 11, the other end of the exhaust branch pipe 16 is fixedly communicated with the exhaust cavity 5, the other end of the air inlet branch pipe 17 is fixedly communicated with the air inlet cavity 6, and a sterilization assembly is installed at the axial position of the maintenance cylinder 11;
the sterilization assembly comprises a glass protective tube 29 arranged at the axial position of the maintenance cylinder 11, an ultraviolet sterilization lamp 30 is fixedly arranged at the axial position of the glass protective tube 29, and an anti-sticking coating is fixedly arranged on the outer wall of the glass protective tube 29 and is a nano anti-sticking coating.
Before bacteria culture solution and bacteria are added, the ultraviolet sterilizing lamp 30 is turned on, after the ultraviolet sterilizing lamp 30 is turned on, ultraviolet sterilizing operation is conducted on the inner walls of the maintenance cylinder 11 and the cultivation cylinder 18, the probability that the bacteria and the bacteria culture are polluted after the bacteria and the bacteria culture are added is effectively reduced through the realization of ultraviolet pre-sterilizing effect, when the bacteria are added and the bacteria are normally cultivated, the ultraviolet sterilizing lamp 30 is kept turned off, after the bacteria and the bacteria solution in the cultivation mechanism 10 are discharged, the ultraviolet sterilizing lamp 30 is turned on, then secondary sterilization is conducted on the inner wall of the cultivation mechanism 10, and through the arrangement of the anti-sticking coating, the wall sticking prevention effect of the bacteria and the bacteria culture solution on the surface of the glass protection tube 29 is effectively improved;
the bottom end of the maintenance cylinder 11 is rotationally communicated with a cultivation cylinder 18, and a water bath component is matched in the cultivation cylinder 18;
the water bath component comprises a water bath annular cavity 31 arranged inside the cultivation barrel 18, a liquid distribution ring pipe 32 connected with the cultivation barrel 18 in a rotating mode and a heating rod 33 fixed inside the heating cavity 7, a group of liquid penetrating holes distributed in a circumferential array mode are formed in the cultivation barrel 18, the liquid distribution ring pipe 32 and the water bath annular cavity 31 are communicated through the liquid penetrating holes, a water bath connecting pipe 34 is fixedly communicated with the top surface of the water bath annular cavity 31, and the other end of the water bath connecting pipe 34 is fixedly communicated with the heating cavity 7.
When the normal cultivation of bacteria is performed, sufficient bath hot water is supplied to both the inside of the water bath annular chamber 31 and the inside of the heating chamber 7, and when the water bath operation is performed, the heating rod 33 operates in a set state, and after the operation of the heating rod 33, the bath hot water is heated, and when the heating rod 33 is heated, the temperature inside the cultivation cylinder 18 is maintained at the set temperature, so that the constant temperature cultivation of bacteria in the cultivation cylinder 18 is realized.
The bottom end of the cultivation cylinder 18 is rotatably connected with a sample outlet shaft cylinder 19, the sample outlet shaft cylinder 19 is of a hollow cylindrical structure with a closed top end and an open bottom end, and the sample outlet shaft cylinder 19 and the cultivation cylinder 18 are both driven by a transmission assembly;
the transmission assembly comprises a bracket fixed on the circumferential side surface of the transposition rotating frame 3 and an inner rotating cylinder 22 rotatably connected to the outer side of the transposition rotating frame 3, the circumferential side surface of the inner rotating cylinder 22 is rotatably connected with an outer rotating cylinder 23, and the circumferential side surfaces of the inner rotating cylinder 22 and the outer rotating cylinder 23 are fixedly provided with transmission gear rings 24;
two side surfaces of the bracket are fixedly provided with transmission motors 25, output shaft ends of the two transmission motors 25 are respectively in transmission connection with the inner rotary cylinder 22 and the outer rotary cylinder 23, peripheral side surfaces of the inner rotary cylinder 22 and the outer rotary cylinder 23 are fixedly provided with driven bevel gear rings, and output shaft ends of the two transmission motors 25 are fixedly provided with driving bevel gears meshed with the driven bevel gear rings;
the peripheral sides of the two transmission gear rings 24 are respectively in transmission connection with the sample outlet shaft cylinder 19 and the culture cylinder 18;
driven gear rings 26 are fixedly arranged on the peripheral side surfaces of the sample outlet shaft cylinder 19 and the culture cylinder 18, and the peripheral side surfaces of the two driven gear rings 26 are respectively meshed with the two transmission gear rings 24;
when the culture device works, the two transmission motors 25 are in an independent working state, namely, the two transmission motors 25 can work independently, when the stirring shaft is required to output rotating speed and carry out stirring operation, the sample outlet shaft cylinder 19 is driven in a set state, then the stirring shaft is driven in the set state to output rotating speed, in the normal culture process of bacteria, the culture cylinder 18 is periodically slowly rotated, and the rotating state of the culture cylinder 18 is set, so that the wall adhesion preventing effect of the bacteria and bacteria culture on the inner wall of the culture cylinder 18 is improved;
a group of weeping holes that are the distribution of circumference array are seted up to the top of going out a kind shaft 19, the top fixed mounting of going out a kind shaft 19 stirs the axle, the week side fixed mounting that stirs the axle has a group of stirring leaf 20 that are the distribution of circumference array, the bottom of going out a kind shaft 19 rotates the intercommunication and has a layout pipe 21, the week side of layout pipe 21 is through linking frame and transposition spiral frame 3 fixed connection, the week side fixed intercommunication of layout pipe 21 has a sampling tube 27, the pipe diameter of sampling tube 27 is 0.1 times of layout pipe 21 pipe diameter.
When the device works, the electromagnetic valves 28 are controlled to be opened by a single chip microcomputer, and the working states of the sample discharge pipe 21, the sampling pipe 27, the sample inlet pipe 14, the culture solution injection pipe 15, the exhaust branch pipe 16 and the air inlet branch pipe 17 are controlled through the arrangement of the electromagnetic valves 28.
When the device is used, the singlechip is arranged to perform central control operation on a corresponding electric mechanism in the device, a bacterial culture to be cultured is added into the culture cylinder 18 from the sample inlet pipe 14, after the bacterial culture is added, enough customized nutrient solution is supplemented into the culture cylinder 18, when the bacterial culture is performed, the humiture probe 12 monitors humiture data in the maintenance cylinder 11 in real time, when the humiture probe 12 works, the monitored real-time humiture data is fed back to the singlechip, the singlechip controls the water bath temperature of the water bath component according to the data feedback of the humiture probe 12, when the bacterial culture is performed, the exhaust branch pipe 16 is used for discharging waste gas generated in the bacterial culture process in the maintenance cylinder 11 at regular time, and the air inlet branch pipe 17 performs oxygen supply operation to the maintenance cylinder 11, then carry out the operation of cultivating the bacterium, the effect that culture solution annotates the pipe 15 setting lies in carrying out the supplementary operation of bacterium culture solution to in maintaining a section of thick bamboo 11, then with the normal operation of cultivating of confession bacterium, when the bacterium culture solution got into, stir axle and vibration exciter 13 and work with setting for the state in step, then stir bacterium and bacterium culture solution, simultaneously in the in-process of bacterium cultivation, stir axle and vibration exciter 13 and open in step, in the bacterium cultivation process, stir the axle and be the low-speed rotation state, then make bacterium and bacterium culture solution distribute evenly, after vibration exciter 13 worked, then make and maintain a section of thick bamboo 11 and a section of thick bamboo 18 of cultivating and produce the stiff vibration frequency, through the production of vibration frequency, thereby further improve the even effect of bacterium and bacterium culture solution in a section of thick bamboo 18 of cultivating.
To sum up: the invention has the advantages that
According to the invention, through the arrangement of the culture mechanism, the transposition rotating frame and other structures, the device can highly absorb bacteria to complete the culture operation of the bacteria, and the device can realize the automatic maintenance of the bacteria culture environment during the bacteria culture, so that the automation efficiency and the automation degree of the device during the bacteria culture are effectively improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. Anti-pollution formula bacteria culture device of microbiological experiment, including frame (1), its characterized in that, the inner wall of frame (1) rotates and is connected with the transposition that drives through rotating electrical machines (2) and revolves frame (3), the transmission assembly is installed to the side of revolving the transposition on the side of frame (3).
2. The microbial experiment anti-pollution type bacteria culture device according to claim 1, wherein a liquid replenishing cavity (4), an air exhausting cavity (5), an air inlet cavity (6) and a heating cavity (7) which are isolated from each other are sequentially formed in the transposition rotating frame (3) from top to bottom, a case (8) is fixedly mounted at the top end of the transposition rotating frame (3), an auxiliary module (9) is mounted inside the case (8), and the top ends of the liquid replenishing cavity (4), the air exhausting cavity (5), the air inlet cavity (6) and the heating cavity (7) are communicated with the auxiliary module (9) through pipelines.
3. The microbial experiment anti-pollution type bacteria culture device according to claim 2, wherein a group of culture mechanisms (10) distributed in a circumferential array is fixedly installed on the circumferential side surface of the transposition rotary frame (3), the culture mechanisms (10) are driven by a transmission assembly, and a port of each culture mechanism (10) is communicated with the liquid supplementing cavity (4), the air exhaust cavity (5), the air inlet cavity (6) and the heating cavity (7).
4. The microorganism experiment anti-pollution type bacteria culture device according to claim 3, wherein the culture mechanism (10) comprises a maintenance cylinder (11) and a single chip microcomputer installed inside the base (1), the inner top of the maintenance cylinder (11) is fixedly provided with a temperature and humidity probe (12), the peripheral side of the maintenance cylinder (11) is fixedly provided with a vibration exciter (13), the top of the maintenance cylinder (11) is fixedly communicated with a sample inlet pipe (14), the peripheral side of the sample inlet pipe (14) is fixedly communicated with a culture solution injection pipe (15), the other end of the culture solution injection pipe (15) is fixedly communicated with a liquid supplement chamber (4), the peripheral side of the maintenance cylinder (11) is respectively and fixedly communicated with an exhaust branch pipe (16) and an air inlet branch pipe (17), the other end of the exhaust branch pipe (16) is fixedly communicated with an exhaust chamber (5), the other end of the air inlet branch pipe (17) is fixedly communicated with a liquid supplement chamber (6), the axis position of the maintenance cylinder (11) is provided with a sterilization assembly, the bottom of the maintenance cylinder (11) rotates to be communicated with a set of the exhaust branch pipe (18), the bottom of the maintenance cylinder (18) is provided with a set of a rotating sample culture cylinder (19), the rotating shaft output assembly is provided with a set of a water bath drive shaft, the sample outlet cylinder (18), and a set of a rotating sample outlet cylinder (18) is provided with a rotating sample culture cylinder (19), and a set of a rotating shaft (18) and a set of a rotating sample outlet cylinder (19) is provided with a rotating sample culture cylinder (18), the sample outlet device is characterized in that a stirring shaft is fixedly mounted at the top end of the sample outlet shaft cylinder (19), a group of stirring blades (20) distributed in a circumferential array manner are fixedly mounted on the peripheral side surface of the stirring shaft, a sample discharge pipe (21) is rotatably communicated with the bottom end of the sample outlet shaft cylinder (19), and the peripheral side surface of the sample discharge pipe (21) is fixedly connected with the transposition rotating frame (3) through a connecting frame; the transmission assembly comprises a support fixed on the peripheral side surface of the transposition rotating frame (3) and an inner rotating cylinder (22) rotatably connected to the outer side of the transposition rotating frame (3), the peripheral side surface of the inner rotating cylinder (22) is rotatably connected with an outer rotating cylinder (23), the peripheral side surfaces of the inner rotating cylinder (22) and the outer rotating cylinder (23) are fixedly provided with transmission gear rings (24), the two side surfaces of the support are fixedly provided with transmission motors (25), the output shaft ends of the two transmission motors (25) are respectively in transmission connection with the inner rotating cylinder (22) and the outer rotating cylinder (23), and the peripheral side surfaces of the two transmission gear rings (24) are respectively in transmission connection with the sample outlet shaft cylinder (19) and the culture cylinder (18); the peripheral side surfaces of the inner rotary cylinder (22) and the outer rotary cylinder (23) are fixedly provided with driven bevel gear rings, and the output shaft ends of the two transmission motors (25) are fixedly provided with driving bevel gears meshed with the driven bevel gear rings.
5. The device for culturing the bacteria in an anti-pollution manner in the microbiological experiment according to claim 4, wherein the sample outlet shaft cylinder (19) and the culture cylinder (18) are fixedly provided with driven gear rings (26) on the circumferential sides, the circumferential sides of the two driven gear rings (26) are respectively engaged with the two transmission gear rings (24), the sample outlet shaft cylinder (19) is a hollow cylindrical structure with a closed top end and an open bottom end, and the sampling tube (27) is fixedly communicated with the circumferential side of the sample discharge tube (21).
6. The device for culturing the bacteria with the anti-pollution function in the microbiological experiment according to claim 5, wherein the electromagnetic valves (28) are arranged inside the sample discharging pipe (21), the sampling pipe (27), the sample inlet pipe (14), the culture solution injection pipe (15), the exhaust branch pipe (16) and the air inlet branch pipe (17).
7. The microbiological experiment anti-pollution type bacteria culture device according to claim 6, wherein the sterilization assembly comprises a glass protective tube (29) installed at the axial position of the maintenance cylinder (11), the axial position of the glass protective tube (29) is fixedly provided with an ultraviolet sterilization lamp (30), the water bath assembly comprises a water bath annular cavity (31) opened inside the cultivation cylinder (18), a liquid distribution annular tube (32) rotatably connected with the cultivation cylinder (18) and a heating rod (33) fixed inside the heating cavity (7), the cultivation cylinder (18) is internally provided with a group of liquid permeable holes distributed in a circumferential array, the liquid distribution annular tube (32) and the water bath annular cavity (31) are communicated through the liquid permeable holes, the top surface of the water bath annular cavity (31) is fixedly communicated with a water bath connecting tube (34), and the other end of the water bath connecting tube (34) is fixedly communicated with the heating cavity (7); the auxiliary module (9) comprises an oxygen storage tank, a waste storage tank, a water inlet pipe and an air pump which are fixedly connected with the chassis (8) respectively, one end of an air inlet of the air pump is fixedly communicated with the exhaust cavity (5), one end of an air outlet of the air pump is fixedly communicated with the waste storage tank, and one end of the air outlet of the oxygen storage tank is fixedly communicated with the air inlet cavity (6).
8. The device for culturing the microorganism experiment anti-pollution type bacteria according to claim 7, wherein an anti-sticking coating is fixedly arranged on the outer wall of the glass protective tube (29), and the ends of the oxygen storage tank, the waste storage tank and the air inlet pipe are communicated with joints.
9. The device according to claim 8, characterized in that the ends of each of said joints extend outside the cabinet (8).
10. The device for culturing the bacteria with the anti-pollution function in the microbiological experiment according to claim 9, wherein an ultraviolet lamp ring (35) and an activated carbon filter element (36) are fixedly installed inside the air inlet cavity (6) from top to bottom in sequence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211719349.1A CN115975774A (en) | 2022-12-30 | 2022-12-30 | Anti-pollution formula bacteria culture device of microbiological assay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211719349.1A CN115975774A (en) | 2022-12-30 | 2022-12-30 | Anti-pollution formula bacteria culture device of microbiological assay |
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| Publication Number | Publication Date |
|---|---|
| CN115975774A true CN115975774A (en) | 2023-04-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211719349.1A Withdrawn CN115975774A (en) | 2022-12-30 | 2022-12-30 | Anti-pollution formula bacteria culture device of microbiological assay |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117264737A (en) * | 2023-10-08 | 2023-12-22 | 刘辉 | Biological fermentation method for microorganisms |
| CN117586855A (en) * | 2023-12-04 | 2024-02-23 | 苏强 | Incubator for laboratory |
-
2022
- 2022-12-30 CN CN202211719349.1A patent/CN115975774A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117264737A (en) * | 2023-10-08 | 2023-12-22 | 刘辉 | Biological fermentation method for microorganisms |
| CN117264737B (en) * | 2023-10-08 | 2024-08-23 | 菏泽学院 | Biological fermentation method for microorganisms |
| CN117586855A (en) * | 2023-12-04 | 2024-02-23 | 苏强 | Incubator for laboratory |
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Application publication date: 20230418 |