CN114768308A - Blood separation device and separation method thereof - Google Patents
Blood separation device and separation method thereof Download PDFInfo
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- CN114768308A CN114768308A CN202210453590.8A CN202210453590A CN114768308A CN 114768308 A CN114768308 A CN 114768308A CN 202210453590 A CN202210453590 A CN 202210453590A CN 114768308 A CN114768308 A CN 114768308A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
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Abstract
The invention provides a blood separation device and a separation method thereof, and relates to the technical field of blood separation. Comprises the following components which are connected in sequence from top to bottom: the first connecting part, the second connecting part and the third connecting part are respectively provided with a PRP locking rod and a red blood cell locking rod which can respectively extend into the PRP cabin and the red blood cell cabin from the through hole and the open hole; sealing elements capable of improving the sealing performance of the first connecting part and the second connecting part and the third connecting part are arranged between the first connecting part and the second connecting part and between the second connecting part and the third connecting part; the blood separation device has the advantages that the blood can be centrifugally separated twice in a state that the blood is not exposed to the air, PRP can be concentrated more, and the PRP can be easily separated, and the gas in the bottom sealing part is compressed and discharged into the side wall sealing part through the gas guide pipe by abutting the bottom sealing part and the limiting part, so that the sealing performance between the side wall sealing part and the inner wall of the second connecting part or the third connecting part is better, and the sealing performance of the whole blood separation device is improved.
Description
Technical Field
The invention relates to the technical field of blood separation, in particular to a blood separation device and a separation method thereof.
Background
Blood, an opaque red fluid that circulates in the heart and vascular cavities; blood belongs to connective tissue and consists of plasma, platelets and blood cells. The blood plasma contains nutritional components such as plasma proteins (albumin, globulin and fibrinogen) and lipoproteins, inorganic salts, oxygen, hormones, enzymes, antibodies, cell metabolites, and the like; the blood cells include red blood cells, white blood cells and platelets; when blood is centrifuged, the blood is separated into Plasma, platelets, and blood cells by their weight, and the platelets separated by this method are formed into Plasma Rich in Plasma, i.e., Platelet Rich Plasma (PRP: Platelet Rich Plasma), and Platelet Poor Plasma, i.e., Platelet Poor Plasma (PPP: Platelet Poor Plasma). Since platelets contain various growth factors, they have important effects on wound healing and skin regeneration.
Since PRP contains the platelet at a high concentration, various growth factors promote the growth of peripheral cells and the sufficient synthesis of components such as collagen. Thus, PRP has recently been used in various fields such as treatment of pain such as lumbago, treatment of alopecia, skin regeneration, and skin diseases such as burn.
However, the separation method using the conventional blood separation apparatus has the following problems in the separation process:
1. in a process of moving the blood to a centrifugal separation container after blood collection and a process of moving the blood to a separation container including a filter unit, the blood is exposed to the air;
2. with the existing blood separation device, it is difficult to concentrate platelets to a high concentration and to accurately extract the required amount of PRP;
3. the existing blood separation device has poor sealing performance among all parts and has the condition of blood leakage.
In view of the above problems, there is a need to design a blood separation device and a separation method thereof for extracting PRP by centrifugation.
Disclosure of Invention
The present invention is directed to a blood separating apparatus and a separating method thereof to solve the above-mentioned problems in the background art.
The embodiment of the invention is realized by the following steps:
on the one hand, this application embodiment provides a blood separator, and it includes from top to bottom consecutive:
a first connecting part, the interior of which is divided into a PRP chamber and a serum chamber by a spacing part and is communicated with the PRP chamber and the serum chamber by a through hole penetrating through the spacing part; openings are formed in the two ends of the PRP cabin and the two ends of the serum cabin, and the openings of the PRP cabin are movably connected with a PRP cabin tail cap; the first connecting part is also provided with an exhaust hole and an injection hole which are communicated with the serum cabin and are respectively sealed through an exhaust hole silica gel cap and an injection hole silica gel cap;
the second connecting part is hollow, two ends of the second connecting part are provided with ports, one end of the second connecting part is movably connected with the other end of the first connecting part, and the second connecting part form the serum cabin;
the upper part of the third connecting part is movably connected with the other end of the second connecting part, the lower part of the third connecting part forms a red blood cell cabin, the lower end of the third connecting part is movably connected with a tail cap of the red blood cell cabin, and the upper part and the lower part of the third connecting part are communicated through an opening;
the second connecting part is internally provided with a PRP locking rod and a red blood cell locking rod which can respectively extend into the PRP cabin and the red blood cell cabin from a through hole and an opening;
sealing elements capable of improving the sealing performance of the first connecting portion and the second connecting portion and the sealing elements capable of improving the sealing performance of the second connecting portion and the third connecting portion are arranged between the first connecting portion and the second connecting portion.
In some embodiments of the present invention, the first connecting portion is provided with an external thread near a lower end thereof, and the second connecting portion is provided with an internal thread on an inner wall of an upper end thereof; the outer wall of the lower end of the second connecting part is provided with an external thread, and the inner wall of the upper end of the third connecting part is provided with an internal thread; and limiting parts abutted against the lower ends of the first connecting part and the second connecting part are arranged in the second connecting part and the third connecting part.
In some embodiments of the present invention, the sealing member includes a side wall sealing portion and a bottom sealing portion connected to each other, the side wall sealing portion abuts against an inner wall of the second connecting portion or the third connecting portion, and the bottom sealing portion abuts against an end surface of the limiting portion;
the side wall sealing part and the bottom sealing part are arranged in a hollow manner and filled with gas media, and the side wall sealing part and the bottom sealing part are made of materials with elastic deformation; one end of the side wall sealing part and one end of the bottom sealing part are fixedly connected with the first connecting part or the second connecting part.
In some embodiments of the present invention, the inside of the side wall sealing part and the bottom sealing part are communicated through a gas-guide tube, and the gas media filled in the side wall sealing part and the bottom sealing part can flow each other through the gas-guide tube.
In some embodiments of the present invention, the sidewall sealing portion is located in an annular groove formed on an outer wall of a bottom end of the first connecting portion and the second connecting portion.
In some embodiments of the present invention, a rotational positioning assembly is disposed between the first connecting portion and the second connecting portion, and between the second connecting portion and the third connecting portion.
In some embodiments of the present invention, the rotational positioning component includes an insert and a plurality of slots;
the plug-in is connected with the outer wall of the first connecting part or/and the outer wall of the second connecting part, and comprises an elastic part and a plug block; the plurality of slots are located in the thread grooves in the inner walls of the second connecting portion or/and the third connecting portion, and are uniformly distributed in the circumferential direction inside the second connecting portion or/and the third connecting portion.
In another aspect, an embodiment of the present application provides a blood separation method applied to any one of the blood separation apparatuses described above, including the following steps:
the first connecting part is upward, and the first connecting part, the second connecting part, the third connecting part, the red blood cell cabin tail cap and the PRP cabin tail cap are adjusted to be connected with each other in a rotating mode;
opening the vent hole, and injecting blood to be separated into the serum cabin;
sealing the exhaust hole through an exhaust hole silica gel cap, and verifying whether the injection hole silica gel cap seals the injection hole;
removing blood attached to the outer walls of the silica gel cap of the exhaust hole and the silica gel cap of the injection hole, and weighing the weight of the first connecting part;
putting the mixture into a centrifuge, keeping the first connecting part vertical, and performing first centrifugation;
opening the silica gel cap of the exhaust hole and the silica gel cap of the injection hole to balance the air pressure inside the first connecting part, the second connecting part and the third connecting part;
rotating the tail cap of the erythrocyte cabin and rotating the third connecting part to enable the erythrocyte locking rod to block the opening hole, and shaking the blood separating device after sealing the exhaust hole and the injection hole;
placing the third connecting part upwards into a centrifuge, keeping the blood separating device vertical, and performing secondary centrifugation;
the first connection part is rotated to extract the PRP from the PRP compartment.
In some embodiments of the present invention, the opening the vent hole and injecting the blood to be separated into the serum compartment further comprises the following steps:
injecting the blood into the silica gel cap of the blood injection port by using an injection needle;
the included angle of the injection needle is maintained at 30 degrees, so that blood is prevented from being stained on the silica gel cap of the exhaust hole and the injection port;
before the injector is pulled out from the injection port, part of air is pumped back and then pulled out.
In some embodiments of the invention, the first centrifugation is 3200RPM/6 minutes; the second centrifugation was 3300RPM/3 minutes.
Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects:
the side wall sealing part is communicated with the bottom sealing part, and the sealing performance between the first connecting part and the second connecting part can be greatly improved when the structure is used; when the first connecting part and the second connecting part or the second connecting part and the third connecting part are connected, the gas in the bottom sealing part can be compressed through the butting between the bottom sealing part and the limiting part, and the gas in the bottom sealing part is discharged into the side wall sealing part through the gas guide tube, so that the sealing property between the side wall sealing part and the inner wall of the second connecting part or the third connecting part is better, and the whole sealing property of the blood separating device is improved;
through the arrangement of the plug-in and the slots, when the first connecting part, the second connecting part or the third connecting part rotates, the rotating angle can be controlled, namely, the rotation is realized through a plurality of evenly distributed slots; the slots are uniformly distributed along the circumferential direction, if 20 slots are arranged, the angle between every two slots is 18 degrees, and when the first connecting part, the second connecting part or the third connecting part are rotated, the rotating angle can be accurately controlled. Similarly, the rotation of the PRP cabin tail cap and the red blood cell cabin tail cap in the first connecting part bracket and the third connecting part bracket can be controlled in the mode;
meanwhile, the separation device can perform centrifugal separation twice under the condition that the blood is not exposed to the air, can concentrate PRP more and can easily perform separation, and can accurately extract the required amount of blood to perform separation by one component.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a schematic structural view of a separation apparatus according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view of a separation device in an embodiment of the invention;
FIG. 3 is a schematic diagram of a second connecting portion according to an embodiment of the present disclosure;
fig. 4 is an enlarged view of a point a in fig. 2.
An icon: 1. a first connection portion; 2. a second connecting portion; 3. a third connecting portion; 4. a PRP cabin; 5. a serum compartment; 6. a PRP cabin tail cap; 7. a red blood cell cabin tail cap; 8. a red blood cell compartment; 9. a PRP locking bar; 10. a red blood cell locking rod; 11. a limiting part; 12. a sidewall seal portion; 13. a bottom seal portion; 14. an air duct; 15. an annular groove; 16. and (4) a slot.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined or explained in subsequent figures.
In the description of the embodiments of the present invention, it should be noted that, if the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or the orientations or positional relationships that the products of the present invention are usually placed in when used, the terms are only used for convenience of description and simplification of the description, and do not indicate or imply that the devices or elements indicated must have specific orientations, be constructed and operated in specific orientations, and thus, should not be construed as limiting the present invention.
Furthermore, the terms "horizontal", "vertical", "suspended" and the like do not require that the components be absolutely horizontal or suspended, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the embodiments of the present invention, "a plurality" means at least 2.
In the description of the embodiments of the present invention, it should be further noted that unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Examples
Referring to fig. 1 to 4, fig. 1 is a schematic structural diagram of a separation device according to an embodiment of the present invention;
FIG. 2 shows a cross-sectional view of a separation device in an embodiment of the invention;
FIG. 3 is a schematic structural diagram of a second connecting portion according to an embodiment of the present invention;
fig. 4 is an enlarged view of a point a in fig. 2.
On the one hand, this application embodiment provides a blood separator, and it includes from top to bottom consecutive:
a first connection part, the interior of which is divided into a PRP chamber and a serum chamber by a spacing part and is communicated with the PRP chamber and the serum chamber by a through hole penetrating through the spacing part; openings are formed in the two ends of the PRP cabin and the two ends of the serum cabin, and the openings of the PRP cabin are movably connected with PRP cabin tail caps; the first connecting part is also provided with an exhaust hole and an injection hole which are communicated with the serum cabin and are respectively sealed by an exhaust hole silica gel cap and an injection hole silica gel cap;
the second connecting part is hollow, and two ends of the second connecting part are provided with ports, wherein one end of the second connecting part is movably connected with the other end of the first connecting part and forms the serum cabin together with the second connecting part;
the upper part of the third connecting part is movably connected with the other end of the second connecting part, the lower part of the third connecting part forms a red blood cell cabin, the lower end of the third connecting part is movably connected with a tail cap of the red blood cell cabin, and the upper part and the lower part of the third connecting part are communicated through an opening;
the PRP locking rod and the erythrocyte locking rod which can respectively extend into the PRP cabin and the erythrocyte cabin from the through hole and the opening are respectively arranged in the second connecting part;
sealing elements capable of improving the sealing performance of the first connecting portion and the second connecting portion and the sealing performance of the second connecting portion and the third connecting portion are arranged between the first connecting portion and the second connecting portion.
In this embodiment, the first connecting portion has an external thread near the lower end thereof, and the second connecting portion has an internal thread on the inner wall of the upper end thereof; the outer wall of the lower end of the second connecting part is provided with an external thread, and the inner wall of the upper end of the third connecting part is provided with an internal thread; all be provided with the spacing portion with the lower extreme butt of first connecting portion and second connecting portion in second connecting portion and the third connecting portion.
In this embodiment, the sealing member includes a side wall sealing portion and a bottom sealing portion connected to each other, the side wall sealing portion abuts against an inner wall of the second connecting portion or the third connecting portion, and the bottom sealing portion abuts against an end surface of the stopper portion;
the side wall sealing part and the bottom sealing part are arranged in a hollow manner and filled with gas media, and both the side wall sealing part and the bottom sealing part are made of materials with elastic deformation; one end of the side wall sealing part and one end of the bottom sealing part are fixedly connected with the first connecting part or the second connecting part. The gas is nitrogen in this embodiment.
In this embodiment, the side wall sealing portion and the bottom sealing portion are communicated with each other through the gas tube, and the gas medium filled in the side wall sealing portion and the bottom sealing portion can flow through the gas tube.
As shown in fig. 2-4, the positions of the side wall sealing part and the bottom sealing part are shown in the figures, and the insides of the side wall sealing part and the bottom sealing part are communicated, so that when the structure is used, the sealing property between the first connecting part and the second connecting part can be greatly improved; when the first connecting portion and the second connecting portion or the second connecting portion and the third connecting portion are connected, the bottom sealing portion and the limiting portion are abutted, the gas inside the bottom sealing portion can be compressed, the gas in the bottom sealing portion is discharged into the side wall sealing portion through the air guide tube, the sealing performance between the side wall sealing portion and the inner wall of the second connecting portion or the inner wall of the third connecting portion is better, and the sealing performance of the whole blood separating device is improved.
In this embodiment, the sidewall sealing portion is located in an annular groove formed in an outer wall of the bottom end of the first connecting portion and the bottom end of the second connecting portion.
Through the setting of annular groove, can make first connecting portion and second connecting portion and the third connecting portion between be connected more convenient, can be better insert wherein.
In this embodiment, a rotational positioning assembly is disposed between the first connecting portion and the second connecting portion, and between the second connecting portion and the third connecting portion.
In this embodiment, the rotational positioning component includes an insert and a plurality of slots;
the plug-in unit is connected with the outer wall of the first connecting part or/and the outer wall of the second connecting part and comprises an elastic unit and a plug block; the plurality of slots are located in the thread grooves in the inner walls of the second connecting portion or/and the third connecting portion, and are evenly distributed along the circumferential direction inside the second connecting portion or/and the third connecting portion.
Through the arrangement of the plug-in and the slots, when the first connecting part, the second connecting part or the third connecting part rotates, the rotating angle can be controlled, namely the rotation is realized through a plurality of uniformly distributed slots; the slots are uniformly distributed along the circumferential direction, if 20 slots are arranged, the angle between every two slots is 18 degrees, and when the first connecting part, the second connecting part or the third connecting part are rotated, the rotating angle can be accurately controlled. Likewise, the rotation within the PRP and erythrocyte stern caps and the first and third connector brackets can also be controlled in this manner.
When the plug-in is actually used, the plug-in can be made of a miniature spring and a plug-in block, and the plug-in block can extend into a plurality of slots and can achieve the limiting effect.
In another aspect, an embodiment of the present application provides a blood separation method applied to any one of the blood separation apparatuses described above, including the following steps:
the first connecting part is upward, and the first connecting part, the second connecting part, the third connecting part, the red blood cell cabin tail cap and the PRP cabin tail cap are adjusted to be connected with each other in a rotating mode;
opening the vent hole, and injecting blood to be separated into the serum cabin;
sealing the exhaust hole through an exhaust hole silica gel cap, and verifying whether the injection hole silica gel cap seals the injection hole;
removing blood attached to the outer walls of the silica gel cap of the exhaust hole and the silica gel cap of the injection hole, and weighing the weight of the first connecting part;
placing the mixture into a centrifuge, keeping the first connecting part vertical, and performing first centrifugation;
opening the silica gel cap of the exhaust hole and the silica gel cap of the injection hole to balance the air pressure inside the first connecting part, the second connecting part and the third connecting part;
rotating the tail cap of the red blood cell cabin and rotating the third connecting part so that the red blood cell locking rod blocks the opening, and shaking the blood separating device after sealing the exhaust hole and the injection hole;
placing the third connecting part upwards into a centrifuge, keeping the blood separation device vertical, and performing second centrifugation;
the first connection part is rotated to extract the PRP from the PRP chamber.
In this embodiment, the step of opening the vent hole and injecting the blood to be separated into the serum compartment further comprises the steps of:
injecting the blood into the silica gel cap of the blood injection port by using an injection needle;
the included angle of the injection needle is maintained at 30 degrees, so that blood is prevented from being stained on the silica gel cap of the exhaust hole and the injection port; when the blood is injected, the needle is inserted a little deep, and the blood flows down along with the PRP locking rod; the short insertion of the needle may cause blood to be drawn into the injection port, resulting in a risk of leakage.
Before the injector is pulled out from the injection port, part of air is pumped back and then pulled out. Namely, the blood left on the injection needle is prevented from being stained on the silica gel cap at the injection port.
In this embodiment, the first centrifugation is 3200RPM/6 minutes; the second centrifugation was 3300RPM/3 minutes.
It will be evident to those skilled in the art that the application is not limited to the details of the foregoing illustrative embodiments, and that the present application may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (10)
1. A blood separating device, characterized in that, including from top to bottom consecutive:
a first connecting part, the interior of which is divided into a PRP chamber and a serum chamber by a spacing part and is communicated with the PRP chamber and the serum chamber by a through hole penetrating through the spacing part; openings are formed in the two ends of the PRP cabin and the two ends of the serum cabin, and the openings of the PRP cabin are movably connected with a PRP cabin tail cap; the first connecting part is also provided with an exhaust hole and an injection hole which are communicated with the serum cabin and are respectively sealed by an exhaust hole silica gel cap and an injection hole silica gel cap;
the second connecting part is hollow, two ends of the second connecting part are provided with ports, one end of the second connecting part is movably connected with the other end of the first connecting part, and the second connecting part form the serum cabin;
the upper part of the third connecting part is movably connected with the other end of the second connecting part, the lower part of the third connecting part forms a red blood cell cabin, the lower end of the third connecting part is movably connected with a tail cap of the red blood cell cabin, and the upper part and the lower part of the third connecting part are communicated through an opening;
the PRP locking rod and the red blood cell locking rod which can respectively extend into the PRP cabin and the red blood cell cabin from a through hole and an opening are respectively arranged in the second connecting part;
and sealing elements capable of improving the sealing performance of the first connecting part and the second connecting part and the third connecting part are arranged between the first connecting part and the second connecting part and between the second connecting part and the third connecting part.
2. A blood separation device according to claim 1 wherein the first connection portion is provided with an external thread adjacent to the lower end thereof and the second connection portion is provided with an internal thread on the inner wall of the upper end thereof; the outer wall of the lower end of the second connecting part is provided with an external thread, and the inner wall of the upper end of the third connecting part is provided with an internal thread; and limiting parts abutted against the lower ends of the first connecting part and the second connecting part are arranged in the second connecting part and the third connecting part.
3. A blood separation apparatus according to claim 2, wherein the sealing member includes a side wall sealing portion and a bottom sealing portion connected to each other, the side wall sealing portion abuts against an inner wall of the second connecting portion or the third connecting portion, and the bottom sealing portion abuts against an end surface of the stopper portion;
the side wall sealing part and the bottom sealing part are arranged in a hollow manner and filled with gas media, and the side wall sealing part and the bottom sealing part are made of materials with elastic deformation; one end of the side wall sealing part and one end of the bottom sealing part are fixedly connected with the first connecting part or the second connecting part.
4. A blood separating apparatus according to claim 3, wherein the inside of the side wall sealing portion and the bottom sealing portion are communicated with each other by a gas guiding tube, and the gas medium filled in the side wall sealing portion and the bottom sealing portion can be made to flow each other by the gas guiding tube.
5. A blood separation device and method according to claim 3 wherein the sidewall seal is located in an annular recess formed in the outer wall of the bottom end of the first and second connection portions.
6. A blood separation device according to claim 1 wherein rotational positioning means are provided between the first and second connection portions and the second and third connection portions.
7. A blood separation device according to claim 6 wherein the rotational positioning assembly comprises a plug and a plurality of slots;
the plug-in is connected with the outer wall of the first connecting part or/and the outer wall of the second connecting part, and comprises an elastic part and a plug block; the plurality of slots are located in the thread grooves in the inner walls of the second connecting portion or/and the third connecting portion, and are uniformly distributed in the circumferential direction inside the second connecting portion or/and the third connecting portion.
8. A blood separation method applied to the blood separation device according to any one of claims 1 to 7, comprising the steps of:
the first connecting part is upward, and the first connecting part, the second connecting part, the third connecting part and the connection between the erythrocyte cabin tail cap and the PRP cabin tail cap are adjusted in a rotating mode;
opening the vent hole and injecting blood to be separated into the serum cabin;
sealing the exhaust hole through the exhaust hole silica gel cap, and verifying whether the injection hole silica gel cap seals the injection hole;
removing blood attached to the outer walls of the silica gel cap of the exhaust hole and the silica gel cap of the injection hole, and weighing the weight of the first connecting part;
putting the mixture into a centrifuge, keeping the first connecting part vertical, and performing first centrifugation;
the air pressure in the first connecting part, the second connecting part and the third connecting part is balanced by opening the silica gel cap of the exhaust hole and the silica gel cap of the injection hole;
rotating the tail cap of the red blood cell cabin and rotating the third connecting part so that the red blood cell locking rod blocks the opening, and shaking the blood separating device after sealing the exhaust hole and the injection hole;
placing the third connecting part upwards into a centrifuge, keeping the blood separating device vertical, and performing secondary centrifugation;
the first connection part is rotated to extract the PRP from the PRP compartment.
9. A method according to claim 8, wherein the step of opening the vent hole and injecting the blood to be separated into the serum compartment further comprises the steps of:
injecting blood into the silica gel cap of the blood injection port directly by using an injection needle;
the included angle of the injection needle head is maintained at 30 degrees, so that blood is prevented from being stained on the silica gel cap of the exhaust hole and the injection port;
before the injector is pulled out from the injection port, partial air is pumped back and then the injector is pulled out.
10. A blood separation method according to claim 8 wherein the first centrifugation is at 3200RPM/6 minutes; the second centrifugation was 3300RPM/3 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210453590.8A CN114768308A (en) | 2022-04-27 | 2022-04-27 | Blood separation device and separation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210453590.8A CN114768308A (en) | 2022-04-27 | 2022-04-27 | Blood separation device and separation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115445683A (en) * | 2022-09-24 | 2022-12-09 | 北京市美嘉亿盛医疗器械股份有限公司 | PRP test tube |
| CN117563277A (en) * | 2024-01-17 | 2024-02-20 | 北京瑞朗泰科医疗器械有限公司 | Preparation device and method for separating platelet-rich plasma from blood |
-
2022
- 2022-04-27 CN CN202210453590.8A patent/CN114768308A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115445683A (en) * | 2022-09-24 | 2022-12-09 | 北京市美嘉亿盛医疗器械股份有限公司 | PRP test tube |
| CN115445683B (en) * | 2022-09-24 | 2023-08-22 | 北京市美嘉亿盛医疗器械股份有限公司 | PRP test tube |
| CN117563277A (en) * | 2024-01-17 | 2024-02-20 | 北京瑞朗泰科医疗器械有限公司 | Preparation device and method for separating platelet-rich plasma from blood |
| CN117563277B (en) * | 2024-01-17 | 2024-04-09 | 北京瑞朗泰科医疗器械有限公司 | Preparation device and method for separating platelet-rich plasma from blood |
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