CN1146781A - 真菌中的核黄素生物合成 - Google Patents
真菌中的核黄素生物合成 Download PDFInfo
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- CN1146781A CN1146781A CN95192767A CN95192767A CN1146781A CN 1146781 A CN1146781 A CN 1146781A CN 95192767 A CN95192767 A CN 95192767A CN 95192767 A CN95192767 A CN 95192767A CN 1146781 A CN1146781 A CN 1146781A
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Abstract
本发明涉及真菌棉阿舒囊霉中的核黄素生物合成基因和用这些基因及基因产物制备核黄素的遗传工程方法。
Description
本发明涉及真菌中核黄素生物合成的基因、其编码的蛋白质和用这些基因和基因产物制备核黄素的遗传工程方法。
已公开了诸如阿舒假囊酵母(Eremothecium ashbyii)或棉阿舒囊霉(Ashbya gossypii)的真菌发酵制备核黄素的方法(The MerckIndex，Windholz等编，Merck & Co.p1183(1983))。
EP405370描述了核黄素高产细菌株，它们是通过枯草芽孢杆菌的核黄素生物合成基因的转化获得的。
因为在细菌和真核细胞中的核黄素生物合成遗传学是不同的，所以上述枯草芽孢杆菌的基因不适用于用诸如棉阿舒囊霉的真核产生菌制备核黄素的重组方法。
于1992年11月19日在德国专利局提交的一项专利申请中，描述了酿酒醇母(Saccharomyces cerevisiae)的核黄素生物合成基因的克隆。
但不可能用酿酒酵母rib基因通过常规杂交方法来克隆棉阿舒囊霉核黄素生物合成基因；因为酿酒醇母和棉阿舒囊霉的rib基因同源性明显不足以杂交。
本发明的一个目的是从真核细胞中分离核黄素生物合成基因，以便提供一种在真核产生菌中制备核黄素的重组方法。
我们发现通过分离见于子囊菌棉阿舒囊霉的6种基因(rib基因)可达到这一目的，这些基因编码从GTP开始的核黄素生物合成酶。
本发明涉及下列DNA序列：
编码具有SEQ ID NO：2所示氨基酸序列的多肽、或编码SEQID NO：2所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
编码具有SEQ ID NO：4所示氨基酸序列的多肽、或编码SEQID NO：4所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
编码具有SEQ ID NO：6所示氨基酸序列的多肽、或编码SEQID NO：6所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
编码具有SEQ ID NO：8所示氨基酸序列的多肽、或编码SEQID NO：8所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
编码具有SEQ ID NO：10所示氨基酸序列的多肽、或编码SEQID NO：10所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
编码具有SEQ ID NO：12所示氨基酸序列的多肽、或编码SEQID NO：12所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
在序列表中显示了这些基因和其基因产物(多肽)的一级结构，并编排如下：
SEQ ID NO：1：rib1基因
SEQ ID NO：2：rib1基因产物(GTP环水解酶II)
SEQ ID NO：3：rib2基因
SEQ ID NO：4：rib2基因产物(DRAP脱氨基酶)
SEQ ID NO：5：rib3基因
SEQ ID NO：6：rib3基因产物(DBP合成酶)
SEQ ID NO：7：rib4基因
SEQ ID NO：8：rib4基因产物(DMRL合成酶)
SEQ ID NO：9：rib5基因
SEQ ID NO：10：rib5基因产物(核黄素合成酶)
SEQ ID NO：11：rib7基因
SEQ ID NO：12：rib7基因产物(HTP还原酶)
鸟苷三磷酸(GTP)通过GTP环水解酶II(rib1基因产物)转变为2，5-二氨基-6-核糖基氨基-4(3H)-嘧啶二酮5-磷酸。然后该化合物通过rib7基因产物还原成2，5-二氨基核糖醇基氨基-2，4(1H，3H)-嘧啶5-磷酸，然后通过rib2基因产物脱氨基成为5-氨基-6-核糖醇基氨基-2，4(1H，3H)-嘧啶二酮。随后在rib4基因产物催化的反应中在该化合物上加上C4化合物DBP，生成6，7-二甲基-8-核糖醇基-2，4-二氧四氢蝶啶(DMRL)，在rib5基因产物催化的反应中从该化合物产生核黄素。C4化合物DBP(L-3，4-二羟基-2-丁酮-4-磷酸)是在rib3基因产物催化的反应中从D-核酮糖5-磷酸形成的。
SEQ ID NO：1、3、5、7、9、11中描述的DNA序列编码SEQ IDNO：2、4、6、8、10、12中描述的多肽。
除序列表中指出的那些外，适用的DNA序列是那些由于遗传密码简并而具有不同的序列但仍编码相同多肽的DNA序列。
本发明还涉及这样的DNA序列，它们编码一级结构与序列表中不同，但仍与序列表中基因产物具有基本相同生物特性的基因产物。生物特性具体指进行核黄素生物合成的酶活性。
这种具有基本相同生物特性的修饰的基因产物可通过缺失或插入一个或多个氨基酸或肽或用其他氨基酸替代氨基酸而获得，或者可从棉阿舒囊霉以外的生物体分离获得。
编码修饰基因产物的DNA序列一般与序列表中所示DNA序列具有80％或更高的同源性。可以用SEQ ID NO：1、3、5、7、9、11中所述DNA序列从棉阿舒囊霉以外的真核细胞中分离这样的DNA序列，例如用常规杂交方法或PCR技术。这些DNA序列在标准条件下与SEQ ID NO：1、3、5、7、9、11中所示DNA序列杂交。
标准条件指：例如浓度为0.1-1×SSC的水性缓中液中(1×SSC：0.15M NaCl，15mM柠檬酸钠，PH7.2)42-58℃。DNA杂交的实验条件在遗传工程的教科书中有述，例如在Sambrook等的“Molecular Cloning”(Cold Spring Harbor haboratory，1989)中。
本发明还涉及调控序列，尤其是启动子序列，它们位于编码适当多肽之DNA序列5′方向的上游。在序列表中具体给出了这些调节序列，并在下文详细解释。
rib1基因的调控序列：
SEQ ID NO：1核苷酸1-242
rib2基因的调控序列：
SEQ ID NO：3核苷酸1-450
rib3基因的调控序列：
SEQ ID NO：5核苷酸1-314
rib4基因的调控序列：
SEQ ID NO：7核苷酸1-270
rib5基因的调控序列：
SEQ ID NO：9核苷酸1-524
rib7基因的调控序列：
SEQ ID NO：11核苷酸1-352
还可以在5′和/或3′方向缩减这些调节序列，而其功能几乎不降低。
调控作用的必需区一般为上述序列区域中的30-100(优选40-70)个核苷酸的片段。
还可以通过与天然序列比较和定向诱变使这些调控序列的功能最优化。
本发明的调控序列适于在Ashbya中过量表达基因，尤其是负责核黄核生物合成的基因。
本发明还涉及含有一种或多种本发明DNA序列的表达载体。通过向本发明DNA序列提供适当的功能调节信号而获得这种表达载体。这种调节信号是负责表达的DNA序列，例如启动子、操纵子、增强子、核糖体结合位点，宿主生物能识别和遵守这些调节信号。
适当时表达载体还可能包含其他如控制重组DNA在宿主生物中的复制或重组的调节信号。
本发明还涉及用本发明的DNA序列或表达载体转化的宿主生物。优选使用真核生物作为宿主生物，特别优选酵母属、假丝酵母属、毕赤酵母属、假囊酵母属或阿舒囊霉属的那些生物。特别优选的种是酿酒酵母、假丝酵母C、flaveri、假丝酵母C、famala、阿舒假囊酵母和棉阿舒囊霉。
本发明还包括制备核黄素的重组方法，其中按常规方法发酵培养本发明的转化宿主生物，从发酵培养基中分离发醇过程中产生的核黄素，和适当时进行纯化。
可按实施例和序列表中所述分离和表征rib基因和基因产物。实施例1棉阿舒囊霉核黄素生物合成基因(rib基因)的分离a.棉阿舒囊霉cDNA文库的构建
在YEPD培养基上培养核黄素高产菌株棉阿舒囊霉ATCC10195后，从对数生长后期的菌丝体中彻底提取RNA(sher-man等，“Methods in Yeast genetics”，cold Spring Harbor，New York，1989)。
通过在oligo(dT)-纤维素上吸附并洗脱两次而纯化Poly(A)+RNA(Aviv and Leder，Proc.Natl，Acrd，Sci，USA 69，1972，1408-1412)。用Gubler和Hoffmann的一般方法(Gene25，1983，263)分离cDNA，在平端cDNA分子的末端加上了合成的ECORI衔接头。然后用T4多核苷酸激酶将EcoRI切割后的cDNA片段磷酸化，并克隆到已用EcoRI切割的去磷酸化载体pYEura3中(图1)。pYEu-ra3(Clonetech Laboratories，Inc.，California)是一种含有半乳糖可诱导的GAL1和GAL10启动子和URA、CEN4和ARS1的酵母表达载体。这些酵母元件使克隆的DNA片段可以在酵母细胞中转化和表达。
连接反应的等分样品用于转化高感受态(Hanahan，DNA Chon-ing ed.D，M，Glover；IRL Press，Oxford1985，109)大肠杆菌XL1-Blue(Bullock等，Biotechniques 5(1987)376-378)，基于其氨苄青霉素抗性筛选转化子。
将3×105个氨苄青霉素抗性细胞合并及扩增，并从中分离质粒DNA(Birnboim and Doly，Nucleic Acids Res.7，1979，1513)。b.分离编码核黄素生产酶的棉阿舒囊霉cDNA克隆。
通过参与核黄素生物合成的酿酒酵母突变体的功能互补分离了编码核黄素生产酶的棉阿舒囊霉cDNA克隆。
菌株AJ88(Mata leu 2 his3 rib1：：URA3 ura 3-52)、AJ115(Matalpha leu 2 inos1 rib 2：：URA 3 ura 3-52)、AJ71(Matalpha leu2 inos1 rib 3：：URA 3 ura 3-52)、AJ106(Matalpha leu2 inos1rib4：：URA3 ura 3-52)、AJ66(Mata canR inos1 rib5：：URA3 ura 3-52)和AJ121(Matalpha leu2 inos1 rib7：：URA3 ura 3-52)是通过破坏酿酒酵母中参与核黄素生物合成的6个基因(rib1-rib5和rib7)之一而产生的突变菌株。
用来自棉阿舒囊霉cDNA文库的25μg cDNA转化每种这些菌株，并铺在含半乳糖而无核黄素的固体培养基上。生长约1周后，从培养皿中分离rib+转化子。
每次分析每种转化突变体(Rib1+、Rib2+、Rib3+、Rib4+、Rib5+和Rib7+)的一个转化子，发现Rib+表型均只在半乳糖培养基中表达而不在葡萄糖培养基中表达。
这些结果证明Rib+表型在质粒中的半乳糖可诱导的GAL10启动子控制下表达。
通过大肠杆菌的转化从Rib1+、Rib2+、Rib3+、Rib4+、Rib5+和Rib7+转化子分离质粒DNA，并称为pJR715、pJR669、pJR788、pJR733、pJR681和pJR827。
对这些质粒中存在的cDNA插入物进行部分测序，表明它们编码的蛋白质类似于酵母的rib基因产物的蛋白质。c.分离编码核黄素生成酶的棉阿舒囊霉基因组克隆
为了分离棉阿舒囊霉的产核黄素基因的基因组拷贝，在粘粒su-per Cosl(Stratagene Cloning Systems，California)中构建了棉阿舒囊霉ATCC 10195的基因组文库，并用从棉阿舒囊霉ribl、rib2、rib3、rib4、rib5和rib7基因cDNA拷贝得到的32p标记的探针进行了筛选。
通过菌落杂交(Grunstein and Hoguess，Proc，Natl，Acad，Sci，USA 72，1975，3961-3965)分离了带有rib1、rib2、rib3、rib4、rib5和rib7DNA的粘粒克隆。用相同的rib特异性cDNA探针对酶切粘粒进行进一步的Southern分析，可能鉴别出含有棉阿舒囊霉rib1、rib2、rib3、rib4、rib5和rib7基因的限制性酶切片段。
发现长3.1kb的Bam HI-ClaI DNA片段含有完整的编码GTP环水解酶II的棉阿舒囊霉rib1基因。用琼脂糖凝胶分离该片段并克隆到用Bam HI和ClaI切割的噬菌粒pBluescript KS(t)(Strata-gene Cloning Systems)中，这样就形成了质粒pJR765(图2)。
获得了一种长1329bp的DNA序列(SEQ ID NO：1)，它含有906bp的rib1开放读码框架、242bp的5′-非编码区和181bp的3′-非编码区。
发现编码DRAP脱氨基酶的完整棉阿舒囊霉rib2基因在长3.0kb的EcoRI-PstI片段上，将此片段克隆到pBluescript ks(t)中得到质粒pJR758(图3)。
对长2627bp的EcoRI-PstI插入区进行了测序，其包括1830bp的rib2开放读码框架、450bp的5′-未翻译区和347bp的3′-未翻译区(SEQ ID NO：3)。
发现编码DBP合成酶的完整棉阿舒囊霉rib3基因存在于长1.5kb的PstI-HindII片段上，将此片段克隆到pBluescript ks(t)中得质粒pJR790(图4)。
对长1082bp的PstI-HindIII插入区进行了测序，其含有639bp的rib3开放读码框架、314bp的5′-未翻译区和129bp的3′-未翻译区(SEQ ID NO：5)。
发现编码DMRL合成酶的棉阿舒囊霉rib4基因存在于长3.2bP的PstI-PstI片段上，将此片段克隆到pBluescript ks(t)中得质粒pJR762(图5)。
对长996bp的PstI-PstI插入区进行了测序，其包括519bp的rib4开放读码框架、270bp的5′-未翻译区和207bp的3′-未翻译区(SEQ ID NO：7)。
发现编码核黄素合成酶的完整棉阿舒囊霉rib5基因存在于长2.5kb的PstI-PstI片段上，将此片段克隆到pBluescript KS(t)中得质粒pJR739(图6)。
对长1511bp的PstI-PstI插入区进行了测序，其包括708bp的rib5开放读码框架、524bp的5′-未翻译区和279bp的3′-未翻译区(SEQ ID NO：9)。
最后，编码HTP还原酶的棉阿舒囊霉rib7基因见于长4.1kb的EcoRI-EcoRI片段上，该片段克隆至pBluescript ks(t)中得质粒pJR845(图7)。
对长1596bp的EcoRI-EcoRI插入区进行了测序，其包括741bp的rib7开放读码框架、352bp的5′-未翻译区和503bp的3′-未翻译区(SEQ ID NO：11)。实施例2
棉阿舒囊霉rib基因的mRNA分析
进行Northern分析鉴定rib-特异性转录本。从实施例1中所述棉阿舒囊霉菌株ATCC10195分离总RNA。将该菌株的RNA样品(5μg)与RNA大小标志物一起在0.8％琼脂糖甲醛凝胶上电泳进行分级分离并真空吸印在尼龙膜上(Thomas，Proc，Natl，Acad，Sci，USA，77.1980，5201-5205)。
将尼龙膜在50％甲酰胺存在下在5×SSC中42℃下分别与32P-标记的rib-特异性DNA探针杂交。棉阿舒囊霉rib1基因表达为约1150核苷酸的单一信使，用来自质粒pJR765的长0.7kbp的SmaI-SacI探针在两个菌株中都检测了mRNA。
与此相似，用长0.5kpb来自pJR758的SmaI-SmaI片段、长0.6kpb来自pJR790的HindIII-kpnI片段、长0.5kpb来自pJR739的ScaI-HindIII片段和长0.3kpb来自pJR845的PstI-PstI片段作为特异探针，在印迹中检测到了单一的长1900核苷酸的rib2、长900核苷酸的rib3、长800核苷酸的rib4、长1050核苷酸的rib5和长1000核苷酸的rib7的转录本。实施例3
棉阿舒囊霉rib基因在酿酒酵母中的表达
如实施例1所述，在核黄素生物合成一个阶段缺陷的已深入研究过的酿酒酵母突变株，如果它们带有编码互补阿舒囊霉属酶的质粒，则可能在没有核黄素的培养基上生长。为了试验棉阿舒囊霉rib基因产物的功能，在来自带有表达质粒pJR715、pJR669、pJR788、pJR733、pJR681和pJR827之一的酿酒酵母突变株的无细胞提取物中测定了黄素产生酶活性。
这些质粒衍生自pYEura3并在实施例1中进行了描述，它们含有半乳糖可诱导型GAL10启动子控制下的棉阿舒囊霉rib特异的cDNA片段。
酿酒酵母的无细胞蛋白质提取物得自在液体培养基中生长至光密度约为2OD的培养液。
收集细胞，用冷的20mM tris HCl(PH7.5)洗涤，并重悬于补有1mM苯乙基磺酰氟的相同缓中液中。
通过在有玻璃珠存在下涡旋并在4℃下3000g离心20分钟分离细胞裂解液。
按文献中所述方法测定GTP环水解酶II、DRAP脱氨基酶、DBP合成酶、DMRL合成酶、核黄素合成酶和HTP还原酶活性(Shavlovsky et al.，Arch.Microbiol.124，1980，255-259；Richter etal.，J.Bacteriol.175，1993，4045-4051；Klein and Bacher，Z.Natur-forsch.35b，1980，482-484；Richter et al.，J.Bacteriol.174，1992，4050-4056；Nielsen et al.，J.Biol. Chem.261，1986，3661；Plaut and Harvey，Methods Enzymol.18B，1971，515-538；Hollanderand Brown，Biochem. Biophys. Res. Commun.89，1979，759-763；Shavlovski et al.，Biochim. Biophys. Acta，428，1976，611-618).
用Peterson法(Anal.Biochem.83，1977，346-356)进行蛋白质定量。如表1中所示，质粒pJR715在酿酒酵母突变株AJ88中表达GTP环水解酶II活性。而且，该活性只存在于生长在半乳糖培养基上的细胞中，这表明棉阿舒囊霉rib1 cDNA的表达发生在半乳糖可诱导型GAL10启动子的控制之下。
这些结果还证明rib1在棉阿舒囊霉中编码GTP环水解酶II。用相似方法证明了在此真菌中rib2编码DRAP脱氨基酶、rib3编码DBP合成酶、rib4编码DMRL合成酶、rib5编码核黄素合成酶及rib7编码HTP还原酶。表1
酿酒酵母rib1突变体AJ88和其转化子的GTP环水解酶II活性。
n.d.未测到。*)野生型**)GTP环水解酶II活性的单位，1U每小时催化1nmol HTP形成表2
| 菌株 | 质粒 | GTP环水解酶IIU/mg蛋白质** | |
| 葡萄糖 | 半乳糖 | ||
| X2180-1A* | — | 0.48 | 0.34 |
| AJ88 | — | n.d. | n.d |
| AJ88 | pIR715 | n.d. | 21.60 |
酿酒酵母rib2突变株AJ115和其转化子的DRAP脱氨基酶活性。
n.d.：未测到。*)1U每小时催化1nmol ARAP形成。表3
| 菌株 | 质粒 | DRAP脱氨基酶U/mg蛋白质* | |
| 葡萄糖 | 半乳糖 | ||
| X2180-1A | - | 0.45 | 0.38 |
| AJ115 | - | n.d. | n.d. |
| AJ115 | pJR669 | n.d. | 53.22 |
酿酒酵母rib3突变株AJ71和其转化子的DBP合成酶活性
n.d.：未测到。*)1U每小时催化1nmol DBP形成。表4
| 菌株 | 质粒 | DBP合成酶U/mg蛋白质* | |
| 葡萄糖 | 半乳糖 | ||
| X2180-1A | - | 0.80 | 0.75 |
| AJ71 | - | n.d. | n.d. |
| AJ71 | pJR788 | n.d. | 25.19 |
酿酒酵母rib4突变株AJ106和其转化子的DMRL合成酶活性
n.d.：未测到。*)1U每小时催化1nmol DMRL形成。表5
| 菌株 | 质粒 | DMRL合成酶U/mg蛋白质* | |
| 葡萄糖 | 半乳糖 | ||
| X2180-1A | - | 2.04 | 1.73 |
| AJ106 | - | n.d. | n.d. |
| AJ106 | pJR733 | n.d. | 86.54 |
酿酒酵母rib5突变株AJ66和其转化子的核黄素合成酶活性
n.d.未测到。*)1U每小时催化1nmol核黄素形成。表6
| 菌株 | 质粒 | 核黄素合成酶U/mg蛋白质* | |
| 葡萄糖 | 半乳糖 | ||
| X2180-1A | — | 4.41 | 3.80 |
| AJ66 | — | n.d. | n.d. |
| AJ66 | pJR681 | n.d. | 164.20 |
酿酒酵母rib7突变体AJ121和其转化子的HTP还原酶活性。
n.d.未测到。*)1U每小时催化1nmol DRAP形成。
| 菌株 | 质粒 | HTP还原酶U/mg蛋白质* | |
| 葡萄糖 | 半乳糖 | ||
| X2180-1A | - | 1.86 | 2.54 |
| AJ121 | - | n.d. | n.d. |
| AJ121 | pJR827 | n.d. | 46.21 |
序列表
(1)一般信息：
(i)申请人：
(A)名称：BASF Aktiengesellschaft
(B)街道：Carl-Bosch-Strasse 38
(C)城市：Ludwigshafen
(E)国家：德国
(F)邮编：D-67056
(G)电话：0621/6048526
(H)传真：0621/6043123
(I)电传：1762175170
(ii)题目：真菌中的核黄素生物合成
(iii)序列数目：12
(iv)计算机可读形式：
(A)媒介类型：软盘
(B)计算机：IBM PC兼容机
(C)操作系统：PC-DOS/MS-DOS
(D)软件：Patenf In Release#1.0，Version#1.25
(EPO)(2)SEQ ID NO：1的信息：
(i)序列特征：
(A)长度：1329碱基对
(B)类型：核酸
(C)链型：双链
(D)拓扑结构：线性
(ii)分子类型：cDNA到mRNA
(iii)假拟：否
(iii)反义：否
(vi)原始来源：
(A)生物体：棉阿舒囊霉
(ix)特征：
(A)名称/关键词：5’UTR
(B)位置：1..242
(ix)特征：
(A)名称/关键词：CDS
(B)位置：243..1148
(ix)特征：
(A)名称/关键词：3’UTR
(B)位置：1149..1329
(xi)序列描述：SEQ ID NO：1：TTTCTGTCCG CATACTTCAT ATGCTCATCG CACATTGATA ATGTACATTC GAAAAATTTC 60AAGATTAGCC TCCGTGAACA GCGATTTACC TTAGGCAAAA GTAACAAAAG GCTTTTCCGT 120AGGTGCTTTG TCATTCAACA ATCCACGTCG GAATTGGCGA CTATATAGTG TAGGGCCCAT 180AAAGCAGTAG TCGGTGTTGA TAGCTGTGTC AGACCAACTC TTTGTTAATT ACTGAAGCTG 240AT ATG ACT GAA TAC ACA GTG CCA GAA GTG AGG TGT GTC GCA CGC GCG 287Met Thr Glu Tyr Thr Val Pro Glu Val Arg Cys Val Ala Arg Ala
1 5 10 15CGC ATA CCG ACG GTA CAG GGC ACC GAT GTC TTC CTC CAT CTA TAC CAC 335Arg Ile Pro Thr Val Gln Gly Thr Asp Val Phe Leu His Leu Tyr His
20 25 30AAC TCG ATC GAC AGC AAG GAA CAC CTA GCG ATT GTC TTC GGC GAG AAC 383Asn Ser Ile Asp Ser Lys Glu His Leu Ala Ile Val Phe Gly Glu Asn
35 40 45ATA CGC TCG CGG AGT CTG TTC CGG TAC CGG AAA GAC GAC ACG CAG CAG 431Ile Arg Ser Arg Ser Leu Phe Arg Tyr Arg Lys Asp Asp Thr Gln Gln
50 55 60GCG CGG ATG GTG CGG GGC GCC TAC GTG GGC CAG CTG TAC CCC GGG CGG 479Ala Arg Met Val Arg Gly Ala Tyr Val Gly Gln Leu Tyr Pro Gly Arg
65 70 75ACC GAG GCA GAC GCG GAT CGG CGT CAG GGC CTG GAG CTG CGG TTT GAT 527Thr Glu Ala Asp Ala Asp Arg Arg Gln Gly Leu Glu Leu Arg Phe Asp80 85 90 95GAG ACA GGG CAG CTG GTG GTG GAG CGG GCG ACG ACG TGG ACC AGG GAG 575Glu Thr Gly Gln Leu Val Val Glu Arg Ala Thr Thr Trp Thr Arg Glu
100 105 110CCG ACA CTG GTG CGG CTG CAC TCG GAG TGT TAC ACG GGC GAG ACG GCG 623Pro Thr Leu Val Arg Leu His Ser Glu Cys Tyr Thr Gly Glu Thr Ala
115 120 125TGG AGC GCG CGG TGC GAC TGC GGG GAG CAG TTC GAC CAG GCG GGT AAG 671Trp Ser Ala Arg Cys Asp Cys Gly Glu Gln Phe Asp Gln Ala Gly Lys
130 135 140CTG ATG GCT GCG GCG ACA GAG GGC GAG GTG GTT GGC GGT GCG GGG CAC 719Leu Met Ala Ala Ala Thr Glu Gly Glu Val Val Gly Gly Ala Gly His
145 150 155GGC GTG ATC GTG TAC CTG CGG CAG GAG GGC CGC GGC ATC GGG CTA GGC 767Gly Val Ile Val Tyr Leu Arg Gln Glu Gly Arg Gly Ile Gly Leu Gly160 165 170 175GAG AAG CTG AAG GCG TAC AAC CTG CAG GAC CTG GGC GCG GAC ACG GTG 815Glu Lys Leu Lys Ala Tyr Asn Leu Gln Asp Leu Gly Ala Asp Thr Val
180 185 190CAG GCG AAC GAG CTG CTC AAC CAC CCT GCG GAC GCG CGC GAC TTC TCG 863Gln Ala Asn Glu Leu Leu Asn His Pro Ala Asp Ala Arg Asp Phe Ser
195 200 205TTG GGG CGC GCA ATC CTA CTG GAC CTC GGT ATC GAG GAC ATC CGG TTG 911Leu Gly Arg Ala Ile Leu Leu Asp Leu Gly Ile Glu Asp Ile Arg Leu
210 215 220CTC ACG AAT AAC CCC GAC AAG GTG CAG CAG GTG CAC TGT CCG CCG GCG 959Leu Thr Asn Asn Pro Asp Lys Val Gln Gln Val His Cys Pro Pro Ala
225 230 235CTA CGC TGC ATC GAG CGG GTG CCC ATG GTG CCG CTT TCA TGG ACT CAG 1007Leu Arg Cys Ile Glu Arg Val Pro Met Val Pro Leu Ser Trp Thr Gln240 245 250 255CCC ACA CAG GGC GTG CGC TCG CGC GAG CTG GAC GGC TAC CTG CGC GCC 1055Pro Thr Gln Gly Val Arg Ser Arg Glu Leu Asp Gly Tyr Leu Arg Ala
260 265 270AAG GTC GAG CGC ATG GGG CAC ATG CTG CAG CGG CCG CTG GTG CTG CAC 1103Lys Val Glu Arg Met Gly His Met Leu Gln Arg Pro Leu Val Leu His
275 280 285ACG TCT GCG GCG GCC GAG CTC CCC CGC GCC AAC ACA CAC ATA TAATCTTTGC 1155Thr Ser Ala Ala Ala Glu Leu Pro Arg Ala Asn Thr His Ile
290 295 300TATATTAAAA CTCTATAAAC GTATGCCACA CGGCGCCCGC GGGCTGCCAC ACGCTGCTCA 1215CGGGCTGCCG AACAGTTCTA ACAAGTAATC GCGCGCCTCG CCAGTGATCG TGGCGAGCAC 1275CTTGTCGTCC ATCATCACAT ATCCTCGGCT ACAGTCGTCG TTGAAGAGCG TGCA 1329(2)SEQ ID NO：2的信息：
(i)序列特征：
(A)长度：301个氨基酸
(B)类型：氨基酸
(D)拓扑结构：线性
(ii)分子类型：蛋白质
(xi)序列描述：SEQ ID NO：2：Met Thr Glu Tyr Thr Val Pro Glu Val Arg Cys Val Ala Arg Ala Arg1 5 10 15Ile Pro Thr Val Gln Gly Thr Asp Val Phe Leu His Leu Tyr His Asn
20 25 30Ser Ile Asp Ser Lys Glu His Leu Ala Ile Val Phe Gly Glu Asn Ile
35 40 45Arg Ser Arg Ser Leu Phe Arg Tyr Arg Lys Asp Asp Thr Gln Gln Ala
50 55 60Arg Met Val Arg Gly Ala Tyr Val Gly Gln Leu Tyr Pro Gly Arg Thr65 70 75 80Glu Ala Asp Ala Asp Arg Arg Gln Gly Leu Glu Leu Arg Phe Asp Glu
85 90 95Thr Gly Gln Leu Val Val Glu Arg Ala Thr Thr Trp Thr Arg Glu Pro
100 105 110Thr Leu Val Arg Leu His Ser Glu Cys Tyr Thr Gly Glu Thr Ala Trp
115 120 125Ser Ala Arg Cys Asp Cys Gly Glu Gin Phe Asp Gln Ala Gly Lys Leu
130 135 140Met Ala Ala Ala Thr Glu Gly Glu Val Val Gly Gly Ala Gly His Gly145 150 155 160Val Ile Val Tyr Leu Arg Gln Glu Gly Arg Gly Ile Gly Leu Gly Glu
165 170 175Lys Leu Lys Ala Tyr Asn Leu Gln Asp Leu Gly Ala Asp Thr Val Gln
180 185 190Ala Asn Glu Leu Leu Asn His Pro Ala Asp Ala Arg Asp Phe Ser Leu
195 200 205Gly Arg Ala Ile Leu Leu Asp Leu Gly Ile Glu Asp Ile Arg Leu Leu
210 215 220Thr Ash Asn Pro Asp Lys Val Gln Gln Val His Cys Pro Pro Ala Leu225 230 235 240Arg Cys Ile Glu Arg Val Pro Met Val Pro Leu Ser Trp Thr Gln Pro
245 250 255Thr Gln Gly Val Arg Ser Arg Glu Leu Asp Gly Tyr Leu Arg Ala Lys
260 265 270Val Glu Arg Met Gly His Met Leu Gln Arg Pro Leu Val Leu His Thr
275 280 285Ser Ala Ala Ala Glu Leu Pro Arg Ala Asn Thr His Ile
290 295 300(2)SEQ ID NO：3的信息：
(i)序列特征：
(A)长度：2627碱基对
(B)类型：核酸
(C)链型：双链
(D)拓扑结构：线性
(ii)分子类型：cDNA到mRNA
(iii)假拟：否
(iii)反义：否
(vi)原始来源：
(A)生物体：棉阿舒囊霉
(ix)特征：
(A)名称/关键词：5’UTR
(B)位置：1..450
(ix)特征：
(A)名称/关键词：CDS
(B)位置：451..2280
(ix)特征：
(A)名称/关键词：3’UTR
(B)位置：2281..2627
(xi)序列描述：SEQ ID NO：3：CTGCAGGACA ATTTAAATTA CGATTACACG CGGCAGCCTT CTTGGTGCGA CAGGATTTTG 60TACAAGAATG ACCCCAAGCG GGTAAGAGTT CATAGGTATG CCTCGATTGA TAGACGTTCC 120ATTTTGAATT ATACTGATCA CGAACCCGTA ACGCTCGATG TCAGCGTTTC ATGCCATACA 180CAATTTGTCC CAATGGCTAT GCAGAATATT TCCCCACAGA GCACCATGGA AATGTATGTG 240GGAGACGTCA CAGATATACT ACTGATGTTG TTCTCCAGAG TATACTACGC CCCTACCATA 300TTCGATCTTG TGGTATTGAC GATATTCCTC TGTTTGGTTT TACTGGCACT ATTCCGTTTG 360ACGGTATAGC GCTATTCGTT CATAGTGACA CATGCGGCAC TAGCTATTCA GCGAATCCTT 420TATAAACTGC TACTTAACGT TCGTAACACC ATG CTC AAA GGC GTT CCT GGC CTT 474
Met Leu Lys Gly Val Pro Gly Leu
1 5CTT TTT AAG GAG ACG CAA CGT CAT CTG AAA CCC AGG CTG GTT AGG ATT 522Leu Phe Lys Glu Thr Gln Arg His Leu Lys Pro Arg Leu Val Arg Ile
10 15 20ATG GAA AAC ACA TCG CAG GAT GAG AGT CGC AAA AGA CAG GTC GCT TCG 570Met Glu Asn Thr Ser Gln Asp Glu Ser Arg Lys Arg Gln Val Ala Ser25 30 35 40AAC TTG AGC AGC GAT GCC GAT GAG GGC TCG CCG GCA GTT ACG AGG CCG 618Asn Leu Ser Ser Asp Ala Asp Glu Gly Ser Pro Ala Val Thr Arg Pro
45 50 55GTT AAA ATC ACC AAA CGC CTC AGG AAG AAG AAC CTC GGG ACA GGC GAG 666Val Lys Ile Thr Lys Arg Leu Arg Lys Lys Asn Leu Gly Thr Gly Glu
60 65 70CTA CGG GAC AAA GCA GGA TTC AAG TTG AAG GTG CAA GAC GTG AGC AAA 714Leu Arg Asp Lys Ala Gly Phe Lys Leu Lys Val Gln Asp Val Ser Lys
75 80 85AAC CGT CAC AGA CAG GTC GAT CCG GAA TAC GAA GTC GTG GTA GAT GGC 762Asn Arg His Arg Gln Val Asp Pro Glu Tyr Glu Val Val Val Asp Gly
90 95 100CCG ATG CGC AAG ATC AAA CCG TAT TTC TTC ACA TAC AAG ACT TTC TGC 810Pro Met Arg Lys Ile Lys Pro Tyr Phe Phe Thr Tyr Lys Thr Phe Cys105 110 115 120AAG GAG CGC TGG AGA GAT CGG AAG TTG CTT GAT GTG TTT GTG GAT GAA 858Lys Glu Arg Trp Arg Asp Arg Lys Leu Leu Asp Val Phe Val Asp Glu
125 130 135TTT CGG GAC CGC GAT AGG CCT TAC TAC GAG AAA GTC ATC GGT TCG GGT 906Phe Arg Asp Arg Asp Arg Pro Tyr Tyr Glu Lys Val Ile Gly Ser Gly
140 145 150GGT GTG CTC CTG AAC GGT AAG TCA TCG ACG TTA GAT AGC GTA TTG CGT 954Gly Val Leu Leu Asn Gly Lys Ser Ser Thr Leu Asp Ser Val Leu Arg
155 160 165AAT GGA GAC CTC ATT TCG CAC GAG CTG CAC CGT CAT GAG CCA CCG GTC 1002Asn Gly Asp Leu Ile Ser His Glu Leu His Arg His Glu Pro Pro Val
170 175 180TCC TCT AGG CCG ATT AGG ACG GTG TAC GAA GAT GAT GAC ATC CTG GTG 1050Ser Ser Arg Pro Ile Arg Thr Val Tyr Glu Asp Asp Asp Ile Leu Val185 190 195 200ATT GAC AAG CCC AGC GGG ATT CCA GCC CAT CCC ACC GGG CGT TAC CGC 1098Ile Asp Lys Pro Ser Gly Ile Pro Ala His Pro Thr Gly Arg Tyr Arg
205 210 215TTC AAC TCC ATT ACG AAA ATA CTT GAA AAA CAG CTT GGA TAC ACT GTT 1146Phe Asn Ser Ile Thr Lys Ile Leu Glu Lys Gln Leu Gly Tyr Thr Val
220 225 230CAT CCA TGT AAC CGA CTG GAC CGC CTA ACC AGT GGC CTA ATG TTC TTG 1194His Pro Cys Asn Arg Leu Asp Arg Leu Thr Ser Gly Leu Met Phe Leu
235 240 245GCA AAA ACT CCA AAG GGA GCC GAT GAG ATG GGT GAT CAG ATG AAG GCG 1242Ala Lys Thr Pro Lys Gly Ala Asp Glu Met Gly Asp Gln Met Lys Ala
250 255 260CGC GAA GTG AAG AAA GAA TAT GTT GCC CGG GTT GTT GGG GAA TTT CCT 1290Arg Glu Val Lys Lys Glu Tyr Val Ala Arg Val Val Gly Glu Phe Pro265 270 275 280ATA GGT GAG ATA GTT GTG GAT ATG CCA CTG AAG ACT ATA GAG CCG AAG 1338Ile Gly Glu Ile Val Val Asp Met Pro Leu Lys Thr Ile Glu Pro Lys
285 290 295CTT GCC CTA AAC ATG GTT TGC GAC CCG GAA GAC GAA GCG GGC AAG GGC 1386Leu Ala Leu Asn Met Val Cys Asp Pro Glu Asp Glu Ala Gly Lys Gly
300 305 310GCT AAG ACG CAG TTC AAA AGA ATC AGC TAC GAT GGA CAA ACG AGC ATA 1434Ala Lys Thr Gln Phe Lys Arg Ile Ser Tyr Asp Gly Gln Thr Ser Ile
315 320 325GTC AAG TGC CAA CCG TAC ACG GGC CGG ACG CAT CAG ATC CGT GTT CAC 1482Val Lys Cys Gln Pro Tyr Thr Gly Arg Thr His Gln Ile Arg Val His
330 335 340TTG CAA TAC CTG GGC TTC CCA ATT GCC AAC GAT CCG ATT TAT TCC AAT 1530Leu Gln Tyr Leu Gly Phe Pro Ile Ala Asn Asp Pro Ile Tyr Ser Asn345 350 355 360CCG CAC ATA TGG GGC CCA AGT CTG GGC AAG GAA TGC AAA GCA GAC TAC 1578Pro His Ile Trp Gly Pro Ser Leu Gly Lys Glu Cys Lys Ala Asp Tyr
365 370 375AAG GAG GTC ATC CAA AAA CTA AAC GAA ATT GGT AAG ACT AAA TCT GCG 1626Lys Glu Val Ile Gln Lys Leu Asn Glu Ile Gly Lys Thr Lys Ser Ala
380 385 390GAA AGT TGG TAC CAT TCT GAT TCC CAA GGT GAA GTT TTC AAA GGG GAA 1674Glu Ser Trp Tyr His Ser Asp Ser Gln Gly Glu Val Phe Lys Gly Glu
395 400 405CAA TGC GAT GAA TGT GGC ACC GAA CTG TAC ACT GAC CCG GGC CCG AAT 1722Gln Cys Asp Glu Cys Gly Thr Glu Leu Tyr Thr Asp Pro Gly Pro Asn
410 415 420GAT CTT GAC TTA TGG TTG CAT GCA TAT CGG TAT GAA TCC ACT GAA CTG 1770Asp Leu Asp Leu Trp Leu His Ala Tyr Arg Tyr Glu Ser Thr Glu Leu425 430 435 440GAT GAG AAC GGT GCT AAA AAG CGG AGT TAC TCT ACT GCG TTT CCT GAG 1818Asp Glu Asn Gly Ala Lys Lys Arg Ser Tyr Ser Thr Ala Phe Pro Glu
445 450 455TGG GCT CTT GAG CAG CAC GGC GAC TTC ATG CGG CTT GCC ATC GAA CAG 1866Trp Ala Leu Glu Gln His Gly Asp Phe Met Arg Leu Ala Ile Glu Gln
460 465 470GCT AAG AAA TGC CCA CCC GCG AAG ACA TCA TTT AGC GTT GGT GCC GTG 1914Ala Lys Lys Cys Pro Pro Ala Lys Thr Ser Phe Ser Val Gly Ala Val
475 480 485TTA GTT AAT GGG ACC GAG ATT TTG GCC ACT GGT TAC TCA CGG GAG CTG 1962Leu Val Asn Gly Thr Glu Ile Leu Ala Thr Gly Tyr Ser Arg Glu Leu
490 495 500GAA GGC AAC ACG CAC GCT GAA CAA TGT GCA CTT CAA AAA TAT TTT GAA 2010Glu Gly Asn Thr His Ala Glu Gln Cys Ala Leu Gln Lys Tyr Phe Glu505 510 515 520CAA CAT AAA ACC GAC AAG GTT CCT ATT GGT ACA GTA ATA TAC ACG ACT 2058Gln His Lys Thr Asp Lys Val Pro Ile Gly Thr Val Ile Tyr Thr Thr
525 530 535ATG GAG CCT TGT TCT CTC CGT CTC AGT GGT AAT AAA CCG TGT GTT GAG 2106Met Glu Pro Cys Ser Leu Arg Leu Ser Gly Asn Lys Pro Cys Val Glu
540 545 550CGT ATA ATC TGC CAG CAG GGT AAT ATT ACT GCT GTT TTT GTT GGC GTA 2154Arg Ile Ile Cys Gln Gln Gly Asn Ile Thr Ala Val Phe Val Gly Val
555 560 565CTT GAG CCA GAC AAC TTC GTG AAG AAC AAT ACA AGT CGT GCG CTA TTG 2202Leu Glu Pro Asp Asn Phe Val Lys Asn Asn Thr Ser Arg Ala Leu Leu
570 575 580GAA CAA CAT GGT ATA GAC TAT ATT CTT GTC CCT GGG TTT CAA GAA GAA 2250Glu Gln His Gly Ile Asp Tyr Ile Leu Val Pro Gly Phe Gln Glu Glu585 590 595 600TGT ACT GAA GCC GCA TTG AAG GGT CAT TGATTTTGCT GCGAATTGTA 2297Cys Thr Glu Ala Ala Leu Lys Gly His
605 610GATGACTTAA AATATCGAGG CGTATAATTC GTCGCATTTT ATATAGTTAT CTATGTTTAC 2357ATGACTGTTT AAGCTTGATC TATATTTCTC AAGTGAATTG CCACATATGT TGGTACGGTA 2417ATAAATTAAT GAGGGAGTTT TGAAATTCGC AACCAATCTT ATATACGTTT GATGATATAA 2477ACGGATTGAG ATTCATTAAG CTACCTGATT TTCGCTGAAC TGTTTGTTAT AGGTTTTTAC 2537AGTAAGATAG TTCCTAAGTT TGTTTATTGT CCCCAGTCGG CCAATTGTTC CGGACTTATT 2597ATTATTACCA TTAGTGGTGT TAGTAGTATT 2627(2)SEQ ID NO：4的信息：
(i)序列特征：
(A)长度：609个氨基酸
(B)类型：氨基酸
(D)拓扑结构：线性
(ii)分子类型：蛋白质
(xi)序列描述：SEQ ID NO：4：Met Leu Lys Gly Val Pro Gly Leu Leu Phe Lys Glu Thr Gln Arg His1 5 10 15Leu Lys Pro Arg Leu Val Arg Ile Met Glu Asn Thr Ser Gln Asp Glu
20 25 30Ser Arg Lys Arg Gln Val Ala Ser Asn Leu Ser Ser Asp Ala Asp Glu
35 40 45Gly Ser Pro Ala Val Thr Arg Pro Val Lys Ile Thr Lys Arg Leu Arg
50 55 60Lys Lys Asn Leu Gly Thr Gly Glu Leu Arg Asp Lys Ala Gly Phe Lys65 70 75 80Leu Lys Val Gln Asp Val Ser Lys Asn Arg His Arg Gln Val Asp Pro
85 90 95Glu Tyr Glu Val Val Val Asp Gly Pro Met Arg Lys Ile Lys Pro Tyr
100 105 110Phe Phe Thr Tyr Lys Thr Phe Cys Lys Glu Arg Trp Arg Asp Arg Lys
115 120 125Leu Leu AsP Val Phe Val Asp Glu Phe Arg Asp Arg Asp Arg Pro Tyr
130 135 140Tyr Glu Lys Val Ile Gly Ser Gly Gly Val Leu Leu Asn Gly Lys Ser145 150 155 160Ser Thr Leu Asp Ser Val Leu Arg Asn Gly Asp Leu Ile Ser His Glu
165 170 175Leu His Arg His Glu Pro Pro Val Ser Ser Arg Pro Ile Arg Thr Val
180 185 190Tyr Glu Asp Asp Asp Ile Leu Val Ile Asp Lys Pro Ser Gly Ile Pro
195 200 205Ala His Pro Thr Gly Arg Tyr Arg Phe Asn Ser Ile Thr Lys Ile Leu
210 215 220Glu Lys Gln Leu Gly Tyr Thr Val His Pro Cys Asn Arg Leu Asp Arg225 230 235 240Leu Thr Ser Gly Leu Met Phe Leu Ala Lys Thr Pro Lys Gly Ala Asp
245 250 255Glu Met Gly Asp Gln Met Lys Ala Arg Glu Val Lys Lys Glu Tyr Val
260 265 270Ala Arg Val Val Gly Glu Phe Pro Ile Gly Glu Ile Val Val Asp Met
275 280 285Pro Leu Lys Thr Ile Glu Pro Lys Leu Ala Leu Asn Met Val Cys Asp
290 295 300Pro Glu Asp Glu Ala Gly Lys Gly Ala Lys Thr Gln Phe Lys Arg Ile305 310 315 320Ser Tyr Asp Gly Gln Thr Ser Ile Val Lys Cys Gln Pro Tyr Thr Gly
325 330 335Arg Thr His Gln Ile Arg Val His Leu Gln Tyr Leu Gly Phe Pro Ile
340 345 350Ala Ash Asp Pro Ile Tyr Ser Asn Pro His Ile Trp Gly Pro Ser Leu
355 360 365Gly Lys Glu Cys Lys Ala Asp Tyr Lys Glu Val Ile Gln Lys Leu Asn
370 375 380Glu Ile Gly Lys Thr Lys Ser Ala Glu Ser Trp Tyr His Ser Asp Ser385 390 395 400Gln Gly Glu Val Phe Lys Gly Glu Gln Cys Asp Glu Cys Gly Thr Glu
405 410 415Leu Tyr Thr Asp Pro Gly Pro Asn Asp Leu Asp Leu Trp Leu His Ala
420 425 430Tyr Arg Tyr Glu Ser Thr Glu Leu Asp Glu Asn Gly Ala Lys Lys Arg
435 440 445Ser Tyr Ser Thr Ala Phe Pro Glu Trp Ala Leu Glu Gln His Gly Asp
450 455 460Phe Met Arg Leu Ala Ile Glu Gln Ala Lys Lys Cys Pro Pro Ala Lys465 470 475 480Thr Ser Phe Ser Val Gly Ala Val Leu Val Asn Gly Thr Glu Ile Leu
485 490 495Ala Thr Gly Tyr Ser Arg Glu Leu Glu Gly Asn Thr His Ala Glu Gln
500 505 510Cys Ala Leu Gln Lys Tyr Phe Glu Gln His Lys Thr Asp Lys Val Pro
515 520 525Ile Gly Thr Val Ile Tyr Thr Thr Met Glu Pro Cys Ser Leu Arg Leu
530 535 540Ser Gly Asn Lys Pro Cys Val Glu Arg Ile Ile Cys Gln Gln Gly Asn545 550 555 560Ile Thr Ala Val Phe Val Gly Val Leu Glu Pro Asp Asn Phe Val Lys
565 570 575Asn Asn Thr Ser Arg Ala Leu Leu Glu Gln His Gly Ile Asp Tyr Ile
580 585 590Leu Val Pro Gly Phe Gln Glu Glu Cys Thr Glu Ala Ala Leu Lys Gly
595 600 605His(2)SEQ ID NO：5的信息：
(i)序列特征：
(A)长度：1082碱基对
(B)类型：核酸
(C)链型：双链
(D)拓扑结构：线性
(ii)分子类型：cDNA到mRNA
(iii)假拟：否
(iii)反义：否
(vi)原始来源：
(A)生物体：棉阿舒囊霉
(ix)特征：
(A)名称/关键词：5’UTR
(B)位置：1..314
(ix)特征：
(A)名称/关键词：CDS
(B)位置：315..953
(ix)特征：
(A)名称/关键词：3’UTR
(B)位置：954..1082
(xi)序列描述：SEQ ID NO：5：CCCTTCTTGC ACGGTCGTTT CTGAAACTCT ACGATTATTG GAACAATGAG TAAGTCCTCA 60AATGTACCAC CTATCTGTAG TTTACTATCG GATTTACTGG CTAAGAGCTG ACCTGTTAGG 120CAAGTGAAAC ATATCACATC GCCAGCAGGT TGGGCTACCA AGGATAGTTG ATGACTTCCA 180TCACCTATAA AAGCGGCTTG AGTGCTTTTG CAATGATTCT GTTCACATGA TGGACAAGAA 240ATACGTACAA AAATTTCAAC GTTTTACAAG TTCCCAAGCT TAGTCAACTC ATCACCAACG 300ACAAACCAAG CAAC ATG ACA AGC CCA TGC ACT GAT ATC GGT ACC GCT ATA 350
Met Thr Set Pro Cys Thr Asp Ile Gly Thr Ala Ile
1 5 10GAG CAG TTC AAG CAA AAT AAG ATG ATC ATC GTC ATG GAC CAC ATC TCG 398Glu Gln Phe Lys Gln Asn Lys Met Ile Ile Val Met Asp His Ile Ser
15 20 25AGA GAA AAC GAG GCC GAT CTA ATA TGT GCA GCA GCG CAC ATG ACT GCC 446Arg Glu Asn Glu Ala Asp Leu Ile Cys Ala Ala Ala His Met Thr Ala
30 35 40GAG CAA ATG GCA TTT ATG ATT CGG TAT TCC TCG GGC TAC GTT TGC GCT 494Glu Gln Met Ala Phe Met Ile Arg Tyr Ser Ser Gly Tyr Val Cys Ala45 50 55 60CCA ATG ACC AAT GCG ATT GCC GAT AAG CTA GAC CTA CCG CTC ATG AAC 542Pro Met Thr Asn Ala Ile Ala Asp Lys Leu Asp Leu Pro Leu Met Asn
65 70 75ACA TTG AAA TGC AAG GCT TTC TCC GAT GAC AGA CAC AGC ACT GCG TAT 590Thr Leu Lys Cys Lys Ala Phe Ser Asp Asp Arg His Ser Thr Ala Tyr
80 85 90ACA ATC ACC TGT GAC TAT GCG CAC GGG ACG ACG ACA GGT ATC TCC GCA 638Thr Ile Thr Cys Asp Tyr Ala His Gly Thr Thr Thr Gly Ile Ser Ala
95 100 105CGT GAC CGG GCG TTG ACC GTG AAT CAG TTG GCG AAC CCG GAG TCC AAG 686Arg Asp Arg Ala Leu Thr Val Asn Gln Leu Ala Asn Pro Glu Ser Lys
110 115 120GCT ACC GAC TTC ACG AAG CCA GGC CAC ATT GTG CCA TTG CGT GCC CGT 734Ala Thr Asp Phe Thr Lys Pro Gly His Ile Val Pro Leu Arg Ala Arg125 130 135 140GAC GGC GGC GTG CTC GAG CGT GAC GGG CAC ACC GAA GCG GCG CTC GAC 782Asp Gly Gly Val Leu Glu Arg Asp Gly His Thr Glu Ala Ala Leu Asp
145 150 155TTG TGC AGA CTA GCG GGT GTG CCA GAG GTC GCT GCT ATT TGT GAA TTA 830Leu Cys Arg Leu Ala Gly Val Pro Glu Val Ala Ala Ile Cys Glu Leu
160 165 170GTA AGC GAA AGG GAC GTC GGG CTG ATG ATG ACT TTG GAT GAG TGT ATA 878Val Ser Glu Arg Asp Val Gly Leu Met Met Thr Leu Asp Glu Cys Ile
175 180 185GAA TTC AGC AAG AAG CAC GGT CTT GCC CTC ATC ACC GTG CAT GAC CTG 926Glu Phe Ser Lys Lys His Gly Leu Ala Leu Ile Thr Val His Asp Leu
190 195 200AAG GCT GCA GTT GCC GCC AAG CAG TAGACGGCAA CGAGTTCTTT AAGTCGGTGT 980Lys Ala Ala Val Ala Ala Lys Gln205 210TCATTTATGT AATATACCAT TTCATCGAAA AAGTCAAATG GTATGAACTA GATTTATCAA 1040TAGTATCTAA GAGTTATGGT ATTCGCAAAA GCTTATCGAT AC 1082(2)SEQ ID NO：6的信息：
(i)序列特征：
(A)长度：212个氨基酸
(B)类型：氨基酸
(D)拓扑结构：线性
(ii)分子类型：蛋白质
(xi)序列描述：SEQ ID NO：6：Met Thr Ser Pro Cys Thr Asp Ile Gly Thr Ala Ile Glu Gln Phe Lys1 5 10 15Gln Asn Lys Met Ile Ile Val Met Asp His Ile Ser Arg Glu Asn Glu
20 25 30Ala Asp Leu Ile Cys Ala Ala Ala His Met Thr Ala Glu Gln Met Ala
35 40 45Phe Met Ile Arg Tyr Ser Ser Gly Tyr Val Cys Ala Pro Met Thr Asn
50 55 60Ala Ile Ala Asp Lys Leu Asp Leu Pro Leu Met Asn Thr Leu Lys Cys65 70 75 80Lys Ala Phe Ser Asp Asp Arg His Ser Thr Ala Tyr Thr Ile Thr Cys
85 90 95Asp Tyr Ala His Gly Thr Thr Thr Gly Ile Ser Ala Arg Asp Arg Ala
100 105 110Leu Thr Val Asn Gln Leu Ala Asn Pro Glu Ser Lys Ala Thr Asp Phe
115 120 125Thr Lys Pro Gly His Ile Val Pro Leu Arg Ala Arg Asp Gly Gly Val
130 135 140Leu Glu Arg Asp Gly His Thr Glu Ala Ala Leu Asp Leu Cys Arg Leu145 150 155 160Ala Gly Val Pro Glu Val Ala Ala Ile Cys Glu Leu Val Ser Glu Arg
165 170 175Asp Val Gly Leu Met Met Thr Leu Asp Glu Cys Ile Glu Phe Ser Lys
180 185 190Lys His Gly Leu Ala Leu Ile Thr Val His Asp Leu Lys Ala Ala Val
195 200 205Ala Ala Lys Gln
210(2)SEQ ID NO：7的信息：
(i)序列特征：
(A)长度：996碱基对
(B)类型：核酸
(C)链型：双链
(D)拓扑结构：线性
(ii)分子类型：cDNA到mRNA
(iii)假拟：否
(iii)反义：否
(vi)原始来源：
(A)生物体：棉阿舒囊霉
(ix)特征：
(A)名称/关键词：5’UTR
(B)位置：1..270
(ix)特征：
(A)名称/关键词：CDS
(B)位置：271..789
(ix)特征：
(A)名称/关键词：3’UTR
(B)位置：790..996
(xi)序列描述：SEQ ID NO：7：TGGTATAATG ATACAGGAAG TGAAAATCCG AAAGGTTCAG ACGATGAAAA GAGTTTGAGA 60CGCATCAATG ATCAGCTTTG AGCTATATGT AAGTCTATTA ATTGATTACT AATAGCAATT 120TATGGTATCC TCTGTTCTGC ATATCGACGG TTCTCACGTG ATGATCAGCT TGAGGCTTCG 180CGGATAAAGT TCCATCGATT ACTATAAAAC CATCACATTA AACGTTCACT ATAGGCATAC 240ACACAGACTA AGTTCAAGTT AGCAGTGACA ATG ATT AAG GGA TTA GGC GAA GTT 294
Met Ile Lys Gly Leu Gly Glu Val
1 5GAT CAA ACC TAC GAT GCG AGC TCT GTC GAG GTT GGC ATT GTC CAC GCG 342Asp Gln Thr Tyr Asp Ala Ser Ser Val Glu Val Gly Ile Val His Ala
10 15 20AGA TGG AAC AAG ACT GTC ATT GAC GCT CTC GAC CAA GGT GCA ATT GAG 390Arg Trp Asn Lys Thr Val Ile Asp Ala Leu Asp Gln Gly Ala Ile Glu25 30 35 40AAA CTG CTT GCT ATG GGA GTG AAG GAG AAG AAT ATC ACT GTA AGC ACC 438Lys Leu Leu Ala Met Gly Val Lys Glu Lys Asn Ile Thr Val Ser Thr
45 50 55GTT CCA GGT GCG TTT GAA CTA CCA TTT GGC ACT CAG CGG TTT GCC GAG 486Val Pro Gly Ala Phe Glu Leu Pro Phe Gly Thr Gln Arg Phe Ala Glu
60 65 70CTG ACC AAG GCA AGT GGC AAG CAT TTG GAC GTG GTC ATC CCA ATT GGA 534Leu Thr Lys Ala Ser Gly Lys His Leu Asp Val Val Ile Pro Ile Gly
75 80 85GTC CTG ATC AAA GGC GAC TCA ATG CAC TTT GAA TAT ATA TCA GAC TCT 582Val Leu Ile Lys Gly Asp Ser Met His Phe Glu Tyr Ile Ser Asp Ser
90 95 100GTG ACT CAT GCC TTA ATG AAC CTA CAG AAG AAG ATT CGT CTT CCT GTC 630Val Thr His Ala Leu Met Asn Leu Gln Lys Lys Ile Arg Leu Pro Val105 110 115 120ATT TTT GGT TTG CTA ACG TGT CTA ACA GAG GAA CAA GCG TTG ACA CGT 678Ile Phe Gly Leu Leu Thr Cys Leu Thr Glu Glu Gln Ala Leu Thr Arg
125 130 135GCA GGC CTC GGT GAA TCT GAA GGC AAG CAC AAC CAC GGT GAA GAC TGG 726Ala Gly Leu Gly Glu Ser Glu Gly Lys His Asn His Gly Glu Asp Trp
140 145 150GGT GCT GCT GCC GTG GAG ATG GCT GTA AAG TTT GGC CCA CGC GCC GAA 774Gly Ala Ala Ala Val Glu Met Ala Val Lys Phe Gly Pro Arg Ala Glu
155 160 165CAA ATG AAG AAG TGAATATTAA AAAATCACTA CTTAAAATTA ACGTTTTTAT 826Gln Met Lys Lys
170TATGTCTATA TCAAATTCTT ACGTGATAAC TTTTGATTTC GCTTCCTGGA TTGGCGCAAG 886GCCTCCCTGT GTCGCAGTTT TTGTTCACGG GTCCACACAG CTCTGTTTTC CCAGAACATA 946TCCTCCCAGC CGGCGAACCG GTTAGACGCT TCTGCTGGCG TTCTTATTTT 996(2)SEQ ID NO：8的信息：
(i)序列特征：
(A)长度：172个氨基酸
(B)类型：氨基酸
(D)拓扑结构：线性
(ii)分子类型：蛋白质
(xi)序列描述：SEQ ID NO：8：Met Ile Lys Gly Leu Gly Glu Val Asp Gln Thr Tyr Asp Ala Ser Ser1 5 10 15Val Glu Val Gly Ile Val His Ala Arg Trp Asn Lys Thr Val Ile Asp
20 25 30Ala Leu Asp Gln Gly Ala Ile Glu Lys Leu Leu Ala Met Gly Val Lys
35 40 45Glu Lys Asn Ile Thr Val Ser Thr Val Pro Gly Ala Phe Glu Leu Pro
50 55 60Phe Gly Thr Gln Arg Phe Ala Glu Leu Thr Lys Ala Ser Gly Lys His65 70 75 80Leu Asp Val Val Ile Pro Ile Gly Val Leu Ile Lys Gly Asp Ser Met
85 90 95His Phe Glu Tyr Ile Ser Asp Ser Val Thr His Ala Leu Met Asn Leu
100 105 110Gln Lys Lys Ile Arg Leu Pro Val Ile Phe Gly Leu Leu Thr Cys Leu
115 120 125Thr Glu Glu Gln Ala Leu Thr Arg Ala Gly Leu Gly Glu Ser Glu Gly
130 135 140Lys His Asn His Gly Glu Asp Trp Gly Ala Ala Ala Val Glu Met Ala145 150 155 160Val Lys Phe Gly Pro Arg Ala Glu Gln Met Lys Lys
165 170(2)SEQ ID NO：9的信息：
(i)序列特征：
(A)长度：1511碱基对
(B)类型：核酸
(C)链型：双链
(D)拓扑结构：线性
(ii)分子类型：cDNA到mRNA
(iii)假拟：否
(iii)反义：否
(vi)原始来源：
(A)生物体：棉阿舒囊霉
(ix)特征：
(A)名称/关键词：5’UTR
(B)位置：1..524
(ix)特征：
(A)名称/关键词：CDS
(B)位置：525..1232
(ix)特征：
(A)名称/关键词：3’UTR
(B)位置：1233..1511
(xi)序列描述：SEQ ID NO：9：TGTATTCAAC CTGGAGGATA ACGAAATTTC CATGGCGCGG GCGATACCAA CCCACAGGAG 60CCAGATATAA GACCAATCCC GGCGGGTGTG CCAGCCGCCA TCAGAGACAG CGGGCCAGCA 120AGGCATGTGA AGTCAAAAGG CGCCAGCTCC TTATCCGCTC CCGCACAAGC AGGACCGGCA 180TATCCCGATG AGCGCGCCAG CACCCAGACG CTACACCACC ATTCGAAGTA GACTTTAAAA 240GAGCGCTTTC CAGCTTCTCA GGCAGTTAGC TCTACGACAA AGGAACCAAG TGATTTTCCC 300GATAGACGCG ACTTGCTCAA CGATGTTTCT GTGACCAGCG CAAGGAGAGA TAGTCCTAAA 360GTATAATCAG ATAGTTAGTC GTATCTTCTA GTTTTATTAG TCAGCTACAT GGCGAACCGC 420CATTTCCTTA TGCATGTCTT ACGAGTTTAA AAAGCTCGCG GTAGCAGAAA AGAAGATGCA 480TAGATGGCAT ACCGAAGCCT ATATCGCCCA TAGAAGTTGA TAGG ATG TTT ACC GGT 536
Met Phe Thr Gly
1ATA GTG GAA CAC ATT GGC ACT GTT GCT GAG TAC TTG GAG AAC GAT GCC 584Ile Val Glu His Ile Gly Thr Val Ala Glu Tyr Leu Glu Asn Asp Ala5 10 15 20AGC GAG GCA GGC GGC AAC GGT GTG TCA GTC CTT ATC AAG GAT GCG GCT 632Ser Glu Ala Gly Gly Asn Gly Val Ser Val Leu Ile Lys Asp Ala Ala
25 30 35CCG ATA CTG GCG GAT TGC CAC ATC GGT GAC TCG ATT GCA TGC AAT GGT 680Pro Ile Leu Ala Asp Cys His Ile Gly Asp Ser Ile Ala Cys Asn Gly
40 45 50ATC TGC CTG ACG GTG ACG GAG TTC ACG GCC GAT AGC TTC AAG GTC GGG 728Ile Cys Leu Thr Val Thr Glu Phe Thr Ala Asp Ser Phe Lys Val Gly
55 60 65ATC GCA CCA GAA ACA GTT TAT CGG ACG GAA GTC AGC AGC TGG AAA GCT 776Ile Ala Pro Glu Thr Val Tyr Arg Thr Glu Val Ser Ser Trp Lys Ala
70 75 80GGC TCC AAG ATC AAC CTA GAA AGG GCC ATC TCG GAC GAC AGG CGC TAC 824Gly Ser Lys Ile Asn Leu Glu Arg Ala Ile Ser Asp Asp Arg Arg Tyr85 90 95 100GGC GGG CAC TAC GTG CAG GGC CAC GTC GAC TCG GTG GCC TCT ATT GTA 872Gly Gly His Tyr Val Gln Gly His Val Asp Ser Val Ala Ser Ile Val
105 110 115TCC AGA GAG CAC GAC GGG AAC TCT ATC AAC TTT AAG TTT AAA CTG CGC 920Ser Arg Glu His Asp Gly Asn Ser Ile Asn Phe Lys Phe Lys Leu Arg
120 125 130GAT CAA GAG TAC GAG AAG TAC GTA GTA GAA AAG GGT TTT GTG GCG ATC 968Asp Gln Glu Tyr Glu Lys Tyr Val Val Glu Lys Gly Phe Val Ala Ile
135 140 145GAC GGT GTG TCG CTG ACT GTA AGC AAG ATG GAT CCA GAT GGC TGT TTC 1016Asp Gly Val Ser Leu Thr Val Ser Lys Met Asp Pro Asp Gly Cys Phe
150 155 160TAC ATC TCG ATG ATT GCA CAC ACG CAG ACC GCT GTA GCC CTT CCA CTG 1064Tyr Ile Ser Met Ile Ala His Thr Gln Thr Ala Val Ala Leu Pro Leu165 170 175 180AAG CCG GAC GGT GCC CTC GTG AAC ATA GAA ACG GAT GTT AAC GGC AAG 1112Lys Pro Asp Gly Ala Leu Val Asn Ile Glu Thr Asp Val Asn Gly Lys
185 190 195CTA GTA GAG AAG CAG GTT GCA CAG TAC CTG AAT GCG CAG CTG GAA GGT 1160Leu Val Glu Lys Gln Val Ala Gln Tyr Leu Asn Ala Gln Leu Glu Gly
200 205 210GAG AGC TCG CCA TTG CAG CGC GTG CTC GAA AGG ATT ATT GAA TCC AAG 1208Glu Ser Ser Pro Leu Gln Arg Val Leu Glu Arg Ile Ile Glu Ser Lys
215 220 225CTT GCT AGC ATC TCA AAT AAG TGATTATATT ATCTTGGGTG CTGTATATCT 1259Leu Ala Ser Ile Ser Asn Lys
230 235TATGTATGTC TTACGACTGT GAATCAGAGG GGTGGCAGCT GGAACACCAG CGACACACCT 1319TCGTCTCCCG CGGTGATCAG CCTTCTGTTT TCCTCAAGTA GTACAAAGTC TAGGACACCC 1379TGTTGTGGCC AACGCAAACA TGGAGCTGCT GCCCGTTACG CACGTCGAAC TCGTAGACCT 1439TGCCGTCAAT GCACGAGGCG AACAGGTGGA AACCGGTGGT CTTGTCAAAC CGCCAGCTTC 1499GTGACCGAGT CC 1511(2)SEQ ID NO：10的信息：
(i)序列特征：
(A)长度：235个氨基酸
(B)类型：氨基酸
(D)拓扑结构：线性
(ii)分子类型：蛋白质
(xi)序列描述：SEQ ID NO：10：Met Phe Thr Gly Ile Val Glu His Ile Gly Thr Val Ala Glu Tyr Leu1 5 10 15Glu Asn Asp Ala Ser Glu Ala Gly Gly Asn Gly Val Ser Val Leu Ile
20 25 30Lys Asp Ala Ala Pro Ile Leu Ala Asp Cys His Ile Gly Asp Ser Ile
35 40 45Ala Cys Asn Gly Ile Cys Leu Thr Val Thr Glu Phe Thr Ala Asp Ser
50 55 60Phe Lys Val Gly Ile Ala Pro Glu Thr Val Tyr Arg Thr Glu Val Ser65 70 75 80Ser Trp Lys Ala Gly Ser Lys Ile Asn Leu Glu Arg Ala Ile Ser Asp
85 90 95Asp Arg Arg Tyr Gly Gly His Tyr Val Gln Gly His Val Asp Ser Val
100 105 110Ala Ser Ile Val Ser Arg Glu His Asp Gly Asn Ser Ile Asn Phe Lys
115 120 125Phe Lys Leu Arg Asp Gln Glu Tyr Glu Lys Tyr Val Val Glu Lys Gly
130 135 140Phe Val Ala Ile Asp Gly Val Ser Leu Thr Val Ser Lys Met Asp Pro145 150 155 160Asp Gly Cvs Phe Tyr Ile Ser Met Ile Ala His Thr Gln Thr Ala Val
165 170 175Ala Leu Pro Leu Lys Pro Asp Gly Ala Leu Val Asn Ile Glu Thr Asp
180 185 190Val Asn Gly Lys Leu Val Glu Lys Gln Val Ala Gln Tyr Leu Asn Ala
195 200 205Gln Leu Glu Gly Glu Ser Ser Pro Leu Gln Arg Val Leu Glu Arg Ile
210 215 220Ile Glu Ser Lys Leu Ala Ser Ile Ser Asn Lys225 230 235(2)SEQ ID NO：11的信息：
(i)序列特征：
(A)长度：1596碱基对
(B)类型：核酸
(C)链型：双链
(D)拓扑结构：线性
(ii)分子类型：cDNA到mRNA
(iii)假拟：否
(iii)反义：否
(vi)原始来源：
(A)生物体：棉阿舒囊霉
(ix)特征：
(A)名称/关键词：5’UTR
(B)位置：1..352
(ix)特征：
(A)名称/关键词：CDS
(B)位置：353..1093
(ix)特征：
(A)名称/关键词：3’UTR
(B)位置：1094..1596
(xi)序列描述：SEQ ID NO：11：AGAAGAAGCG CAGGCGCCAG TCCGAGCTGG AGGAGAACGA GGCGGCGCGG TTGACGAACA 60GCGCGCTGCC CATGGACGAT GCGGGTATAC AGACGGCGGG TATACAGACG GCGGGTGGTG 120CCGAGAGAGG CACCAGGCCG GCTTCCTCCA GCGATGCAAG GAAGAGAAGG GGACCAGAGG 180CGAAGTTCAA GCCATCTAAG GTACAGAAGC CCCAATTGAA GCGAACTGCA TCGTCCCGGG 240CGGATGAGAA CGAGTTCTCG ATATTATAGA GGCCCCCGTT TCGAGTGATT GGCGTCAAAA 300ACGGCTATCT GCCTTCGTCC GCCCCCACCA CCCTCGGGAA CACTGGCAAA CC ATG 355
Met
1GCG CTA ATA CCA CTT TCT CAA GAT CTG GCT GAT ATA CTA GCA CCG TAC 403Ala Leu Ile Pro Leu Ser Gln Asp Leu Ala Asp Ile Leu Ala Pro Tyr
5 10 15TTA CCG ACA CCA CCG GAC TCA TCC GCA CGC CTG CCG TTT GTC ACG CTG 451Leu Pro Thr Pro Pro Asp Ser Ser Ala Arg Leu Pro Phe Val Thr Leu
20 25 30ACG TAT GCG CAG TCC CTA GAT GCT CGT ATC GCG AAG CAA AAG GGT GAA 499Thr Tyr Ala Gln Ser Leu Asp Ala Arg Ile Ala Lys Gln Lys Gly Glu
35 40 45AGG ACG GTT ATT TCG CAT GAG GAG ACC AAG ACA ATG ACG CAT TAT CTA 547Arg Thr Val Ile Ser His Glu Glu Thr Lys Thr Met Thr His Tyr Leu50 55 60 65CGC TAC CAT CAT AGC GGC ATC CTG ATT GGC TCG GGC ACA GCC CTT GCG 595Arg Tyr His His Ser Gly Ile Leu Ile Gly Ser Gly Thr Ala Leu Ala
70 75 80GAC GAC CCG GAT CTC AAT TGC CGG TGG ACA CCT GCA GCG GAC GGG GCG 643Asp Asp Pro Asp Leu Asn Cys Arg Trp Thr Pro Ala Ala Asp Gly Ala
85 90 95GAT TGC ACC GAA CAG TCT TCA CCA CGA CCC ATT ATC TTG GAT GTT CGG 691Asp Cys Thr Glu Gln Ser Ser Pro Arg Pro Ile Ile Leu Asp Val Arg
100 105 110GGC AGA TGG AGA TAC CGC GGG TCC AAA ATA GAG TAT CTG CAT AAC CTT 739Gly Arg Trp Arg Tyr Arg Gly Ser Lys Ile Glu Tyr Leu His Asn Leu
115 120 125GGC AAG GGG AAG GCG CCC ATA GTG GTC ACG GGG GGT GAG CCG GAG GTC 787Gly Lys Gly Lys Ala Pro Ile Val Val Thr Gly Gly Glu Pro Glu Val130 135 140 145CGC GAA CTA GGC GTC AGT TAC CTG CAG CTG GGT GTC GAC GAG GGT GGC 835Arg Glu Leu Gly Val Ser Tyr Leu Gln Leu Gly Val Asp Glu Gly Gly
150 155 160CGC TTG AAT TGG GGC GAG TTG TTT GAG CGA CTC TAT TCT GAG CAC CAC 883Arg Leu Asn Trp Gly Glu Leu Phe Glu Arg Leu Tyr Ser Glu His His
165 170 175CTG GAA AGT GTC ATG GTC GAA GGC GGC GCG GAG GTG CTC AAC CAG CTG 931Leu Glu Ser Val Met Val Glu Gly Gly Ala Glu Val Leu Asn Gln Leu
180 185 190CTG CTG CGC CCA GAT ATT GTG GAC AGT CTG GTG ATC ACG ATA GGA TCC 979Leu Leu Arg Pro Asp Ile Val Asp Ser Leu Val Ile Thr Ile Gly Ser
195 200 205AAG TTC CTG GGC TCA CTA GGT GTT GCG GTC TCA CCA GCT GAG GAG GTG 1027Lys Phe Leu Gly Ser Leu Gly Val Ala Val Ser Pro Ala Glu Glu Val210 215 220 225AAC CTA GAG CAT GTG AAC TGG TGG CAC GGA ACA AGT GAC AGT GTT TTG 1075Asn Leu Glu His Val Asn Trp Trp His Gly Thr Ser Asp Ser Val Leu
230 235 240TGC GGC CGG CTC GCA TAGCGGTTAT GACTGGTCTA CTAGTTAAAA CTATTTACTC 1130Cys Gly Arg Leu Ala
245CTATACATAT TGCGTCACAT AGCGTTTATC CCCCTCGCCA ACCGCCTCGT GCCGTTGGAA 1190ACACGGCGGC CGGGGGACCT CAAGCGCTCC GCATCGACTA GTTTAATTTA CAAACAGATT 1250CTGTAACTTG CGTAACGGCC AGAGGTCTCT GACTTTCTGA TAATCTTCAC CACCTCACCT 1310CGCTTCAACC CCAGGTATAA TGCAACTTGG ATCCATCCTC TGGATTCTAG GTAACTGAGA 1370TTCCTTTAAC CTGTATCTCT TCAACAACTC CTTCTTTTCT TCGTCGCTGA GTTTGATATG 1430TTTTGGCACA AGCTCATGGT GCGTGATATT TACCACCAAA GCTGTTTCGT TGAAAGTCTC 1490AATTGTAGCA GGAGCGACGG AGGGAAGCAG TTTCAACGCG CTGGGCGTTA TGCCGTTCTG 1550ATATATGAAA ATACCCGTCT GGAAGTTCTT CTCGCCAATG TGGATC 1596(2)SEQ ID NO：12的信息：
(i)序列特征：
(A)长度：246个氨基酸
(B)类型：氨基酸
(D)拓扑结构：线性
(ii)分子类型：蛋白质
(xi)序列描述：SEQ ID NO：12：Met Ala Leu Ile Pro Leu Ser Gln Asp Leu Ala Asp Ile Leu Ala Pro1 5 10 15Tyr Leu Pro Thr Pro Pro Asp Ser Ser Ala Arg Leu Pro Phe Val Thr
20 25 30Leu Thr Tyr Ala Gln Ser Leu Asp Ala Arg Ile Ala Lys Gln Lys Gly
35 40 45Glu Arg Thr Val Ile Ser His Glu Glu Thr Lys Thr Met Thr His Tyr
50 55 60Leu Arg Tyr His His Ser Gly Ile Leu Ile Gly Ser Gly Thr Ala Leu65 70 75 80Ala Asp Asp Pro Asp Leu Asn Cys Arg Trp Thr Pro Ala Ala Asp Gly
85 90 95Ala Asp Cys Thr Glu Gln Ser Ser Pro Arg Pro Ile Ile Leu Asp Val
100 105 110Arg Gly Arg Trp Arg Tyr Arg Gly Ser Lys Ile Glu Tyr Leu His Asn
115 120 125Leu Gly Lys Gly Lys Ala Pro Ile Val Val Thr Gly Gly Glu Pro Glu
130 135 140Val Arg Glu Leu Gly Val Ser Tyr Leu Gln Leu Gly Val Asp Glu Gly145 150 155 160Gly Arg Leu Asn Trp Gly Glu Leu Phe Glu Arg Leu Tyr Ser Glu His
165 170 175His Leu Glu Ser Val Met Val Glu Gly Gly Ala Glu Val Leu Asn Gln
180 185 190Leu Leu Leu Arg Pro Asp Ile Val Asp Ser Leu Val Ile Thr Ile Gly
195 200 205Ser Lys Phe Leu Gly Ser Leu Gly Val Ala Val Ser Pro Ala Glu Glu
210 215 220Val Asn Leu Glu His Val Asn Trp Trp His Gly Thr Ser Asp Ser Val225 230 235 240Leu Cys Gly Arg Leu Ala
245
Claims (9)
1.编码具有SEQ ID NO：2所示氨基酸序列的多肽、或编码SEQ ID NO：2所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
2.编码具有SEQ ID NO：4所示氨基酸序列的多肽、或编码SEQ ID NO：4所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
3.编码具有SEQ ID NO：6所示氨基酸序列的多肽、或编码SEQ ID NO：6所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
4.编码具有SEQ ID NO：8所示氨基酸序列的多肽、或编码SEQ ID NO：8所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
5.编码具有SEQ ID NO：10所示氨基酸序列的多肽、或编码SEQ ID NO：10所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
6.编码具有SEQ ID NO：12所示氨基酸序列的多肽、或编码SEQ ID NO：12所示多肽的类似物或衍生物的DNA序列，所述类似物或衍生物中一个或多个氨基酸已被缺失、插入或被其他氨基酸所替代、而基本不降低该多肽的酶促作用。
7.含有一种或多种权利要求1-6中的DNA序列的表达载体。
8.已被权利要求7的表达系统转化的宿主生物。
9.制备核黄素的重组方法，该方法利用权利要求8的宿主生物。
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP4410382.4 | 1994-03-25 | ||
| DE4410382 | 1994-03-25 | ||
| DE4420785A DE4420785A1 (de) | 1994-03-25 | 1994-06-15 | Riboflavin-Biosynthese in Pilzen |
| DEP4420785.9 | 1994-06-15 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1146781A true CN1146781A (zh) | 1997-04-02 |
| CN1117151C CN1117151C (zh) | 2003-08-06 |
Family
ID=25935071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95192767A Expired - Lifetime CN1117151C (zh) | 1994-03-25 | 1995-03-15 | 真菌中的核黄素生物合成 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5821090A (zh) |
| EP (1) | EP0751995B1 (zh) |
| JP (1) | JP3756180B2 (zh) |
| CN (1) | CN1117151C (zh) |
| CA (1) | CA2186403C (zh) |
| DK (1) | DK0751995T3 (zh) |
| ES (1) | ES2216010T3 (zh) |
| WO (1) | WO1995026406A2 (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8968093B2 (en) | 2004-07-15 | 2015-03-03 | Intel Corporation | Dynamic insertion of personalized content in online game scenes |
| CN114592000A (zh) * | 2020-12-03 | 2022-06-07 | 上海市农业科学院 | 一种六基因组合在水稻种子中提高vb2含量的应用及方法 |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6350597B1 (en) | 1994-01-13 | 2002-02-26 | E. I. Du Pont De Nemours & Company | Riboflavin synthase genes and enzymes and methods of use |
| CN1110569C (zh) * | 1995-07-13 | 2003-06-04 | 巴斯福股份公司 | 利用异柠檬酸裂合酶活性被改变的微生物制备核黄素 |
| US6239264B1 (en) | 1996-12-31 | 2001-05-29 | Syngenta Participations Ag | Genomic DNA sequences of ashbya gossypii and uses thereof |
| US6146866A (en) * | 1997-08-15 | 2000-11-14 | E.I. Du Pont De Nemours And Company | Lumazine synthase and riboflavin synthase |
| US6323013B1 (en) | 1998-10-28 | 2001-11-27 | E. I. Du Pont De Nemours And Company | Lumazine synthase and riboflavin synthase |
| US6682891B2 (en) | 1997-08-15 | 2004-01-27 | E. I. Du Pont De Nemours And Company | Methods of identifying nucleic acid sequences encoding plant riboflavin synthase enzymes |
| DE19840709A1 (de) * | 1997-12-22 | 1999-06-24 | Forschungszentrum Juelich Gmbh | Ein- oder mehrzellige Organismen zur Herstellung von Riboflavin |
| EP0927761A3 (de) * | 1997-12-23 | 2001-09-05 | Basf Aktiengesellschaft | Gene der Purinbiosyntese aus Ashbya gossypii und deren Verwendung in der mikrobiellen Riboflavinsynthese |
| DE19801120A1 (de) | 1998-01-15 | 1999-07-22 | Basf Ag | Orotidin-5'-Phosphatdecarboxylase-Gen, Genkonstrukt enthaltend dieses Gen und seine Verwendung |
| ID23301A (id) | 1998-04-23 | 2000-04-05 | Hoffmann La Roche | Produksi yang berlebihan dari riboflavin di dalam ragi |
| US6074830A (en) * | 1998-06-09 | 2000-06-13 | E. I. Du Pont De Nemours & Company | 3,4-dihydroxy-2-butanone 4-phosphate synthase |
| US6291660B1 (en) | 1998-10-08 | 2001-09-18 | Syngenta Participations Ag | Fungal genes required for normal growth and development |
| US7009045B2 (en) * | 2000-07-14 | 2006-03-07 | Archer-Daniels-Midland Company | Transformation systems for flavinogenic yeast |
| US20050221460A1 (en) * | 2001-08-22 | 2005-10-06 | Basf Ag | Novel genetic products from ashbya gossypii, associated with the structure of the cell wall or the cytoskeleton |
| KR20040032999A (ko) * | 2001-08-29 | 2004-04-17 | 바스프 악티엔게젤샤프트 | 전사 메카니즘, ｒｎａ 프로세싱 및(또는) 번역과 관련된,아쉬비아 고쉬피로부터의 신규 유전자 산물 |
| DE10159396A1 (de) * | 2001-12-04 | 2003-06-12 | Basf Ag | Genetische Stammoptimierung zur verbesserten Herstellung von Riboflavin |
| DE10209363A1 (de) * | 2002-03-02 | 2003-09-11 | Basf Ag | Verfahren zur Herstellung von Riboflavin |
| DE60314258T2 (de) * | 2002-09-06 | 2008-02-07 | Basf Ag | Gtp cyclohydrolase ii als ziel für fungizide |
| KR100422305B1 (ko) | 2002-12-05 | 2004-03-10 | 씨제이 주식회사 | 리보플라빈을 생산하는 미생물 및 이를 이용한리보플라빈의 생산방법 |
| KR100422307B1 (ko) | 2002-12-05 | 2004-03-10 | 씨제이 주식회사 | 리보플라빈을 생산하는 미생물 및 이를 이용한리보플라빈의 생산방법 |
| KR101227226B1 (ko) * | 2004-07-07 | 2013-01-28 | 디에스엠 아이피 어셋츠 비.브이. | 개선된 효소 |
| KR101335853B1 (ko) | 2011-12-01 | 2013-12-02 | 씨제이제일제당 (주) | Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법 |
| KR102561863B1 (ko) * | 2014-11-19 | 2023-08-01 | 바스프 에스이 | Imp 데히드로게나아제 활성을 증가시키기 위한 에레모테시움의 유전적 변형 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066486C (zh) * | 1989-06-22 | 2001-05-30 | 霍夫曼-拉罗奇有限公司 | 高产核黄素的细菌菌株 |
| DE59310239D1 (de) * | 1992-05-11 | 2002-01-10 | Basf Ag | DNA-Verbindungen und rekombinante, die Ribloflavinsynthetaseaktivität von S. cerevisiae codierende DNA-Expressionsvektoren |
| DE4238904A1 (de) * | 1992-11-19 | 1994-05-26 | Basf Ag | Riboflavin-Synthese in Hefen |
-
1995
- 1995-03-15 WO PCT/EP1995/000958 patent/WO1995026406A2/de active IP Right Grant
- 1995-03-15 DK DK95913119T patent/DK0751995T3/da active
- 1995-03-15 JP JP52493295A patent/JP3756180B2/ja not_active Expired - Fee Related
- 1995-03-15 ES ES95913119T patent/ES2216010T3/es not_active Expired - Lifetime
- 1995-03-15 CA CA002186403A patent/CA2186403C/en not_active Expired - Lifetime
- 1995-03-15 EP EP95913119A patent/EP0751995B1/de not_active Expired - Lifetime
- 1995-03-15 CN CN95192767A patent/CN1117151C/zh not_active Expired - Lifetime
- 1995-03-15 US US08/716,301 patent/US5821090A/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8968093B2 (en) | 2004-07-15 | 2015-03-03 | Intel Corporation | Dynamic insertion of personalized content in online game scenes |
| CN114592000A (zh) * | 2020-12-03 | 2022-06-07 | 上海市农业科学院 | 一种六基因组合在水稻种子中提高vb2含量的应用及方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1995026406A2 (de) | 1995-10-05 |
| EP0751995A1 (de) | 1997-01-08 |
| CA2186403A1 (en) | 1995-10-05 |
| JP3756180B2 (ja) | 2006-03-15 |
| WO1995026406A3 (de) | 2001-12-20 |
| EP0751995B1 (de) | 2004-02-18 |
| DK0751995T3 (da) | 2004-03-08 |
| CN1117151C (zh) | 2003-08-06 |
| ES2216010T3 (es) | 2004-10-16 |
| CA2186403C (en) | 2006-01-24 |
| US5821090A (en) | 1998-10-13 |
| JPH09510618A (ja) | 1997-10-28 |
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