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CN114503998B - Insecticide and preparation method and application thereof - Google Patents

Insecticide and preparation method and application thereof Download PDF

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Publication number
CN114503998B
CN114503998B CN202210038640.6A CN202210038640A CN114503998B CN 114503998 B CN114503998 B CN 114503998B CN 202210038640 A CN202210038640 A CN 202210038640A CN 114503998 B CN114503998 B CN 114503998B
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China
Prior art keywords
phosphatidylinositol
phosphatidylethanolamine
phosphatidylcholine
insecticide
pests
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CN114503998A (en
Inventor
刘勇
李志强
黄莉萍
严硕
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Hunan Aigli Biotechnology Co ltd
HUNAN PLANT PROTECTION INSTITUTE
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Hunan Aigli Biotechnology Co ltd
HUNAN PLANT PROTECTION INSTITUTE
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Forests & Forestry (AREA)
  • General Health & Medical Sciences (AREA)
  • Ecology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides an insecticide, a preparation method and application thereof, wherein the insecticide comprises one or more of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine. The preparation method comprises the following steps: dissolving one or more of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine in water to obtain the pesticide. In the invention, phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine are respectively sprayed on plant leaves in different concentrations, so that the quick and massive death of pests such as bemisia tabaci, thrips, aphids, brown planthoppers and the like can be caused in a short time, and the pesticide can be applied to pest control.

Description

Insecticide and preparation method and application thereof
Technical Field
The invention belongs to the field of agricultural products, and relates to an insecticide, a preparation method and application thereof.
Background
The field pests are various, and various pests such as bemisia tabaci, aphids, thrips and the like can occur on one crop at the same time, and the insects can transmit viruses at the same time, so that serious harm is caused to crop production. At present, conventional prevention and control measures mainly depend on chemical pesticides, but misuse or disorder pesticides in production lead to pest resistance development, excessive pesticide residues and environmental pollution of vegetable products, and lack of medicaments capable of simultaneously preventing and controlling various pests.
There is a need for an insecticide that can kill multiple pests simultaneously and that is beneficial to human health.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provide an insecticide and a preparation method and application thereof. The three components of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine in the pesticide are respectively sprayed on plant leaves in different concentrations, and can cause rapid and massive death of pests such as bemisia tabaci, thrips, aphids, brown planthoppers and the like in a short time.
In order to solve the technical problems, the invention provides an insecticide, wherein the insecticide comprises one or more of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine.
The pesticide further comprises 0.5-20 g/L phosphatidylcholine and 1-20 g/L phosphatidylinositol; the concentration of the phosphatidylethanolamine is 1-20 g/L.
The pesticide further comprises 1-10 g/L of phosphatidylcholine and 1-10 g/L of phosphatidylinositol.
The pesticide further comprises 1-10 g/L of phosphatidylcholine and 1-10 g/L of phosphatidylethanolamine.
The pesticide further comprises 0.5-10 g/L phosphatidylinositol and 0.5-10 g/L phosphatidylethanolamine.
The pesticide further comprises 0.1-10 g/L of phosphatidylcholine, 0.1-10 g/L of phosphatidylinositol and 0.1-10 g/L of phosphatidylethanolamine.
Based on a general technical concept, the invention also provides a preparation method of the pesticide, which comprises the following steps:
dissolving one or more of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine in water to obtain the pesticide.
Based on a general technical concept, the invention also provides application of the pesticide in pest control.
The application, further, the application method is as follows: spraying insecticide on the leaves of the plants, and killing the pests by feeding the pests on the leaves.
The application, further, the pests are one or more of bemisia tabaci, thrips, aphids and brown planthoppers.
Compared with the prior art, the invention has the advantages that:
(1) The invention provides an insecticide, the applicant discovers for the first time that phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine which are active ingredients of soybean extract lecithin can cause higher mortality of different pests at different concentrations, and the lecithin is a substance beneficial to human beings, so that the insecticide can be developed by utilizing the characteristic, various pests can be killed at the same time, the insecticide is beneficial to human health, and a reference is provided for developing green nuisance-free insecticide.
(2) The invention provides a preparation method of an insecticide, which has simple preparation process and can be used for industrial production.
(3) The invention provides an application of an insecticide in preventing plant pests, wherein the component is from soybean lecithin, which not only can kill the pests, but also is beneficial to human health, and belongs to a green nuisance-free insecticide.
Drawings
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
FIG. 1 shows the results of examining the number of live bemisia tabaci on leaves after 24 hours of spraying water, phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine alone in experiment one of the present invention.
Detailed Description
The invention is further described below in connection with specific preferred embodiments, but it is not intended to limit the scope of the invention.
The materials and instruments used in the examples below are all commercially available, with pure products of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine, available from Shanghai microphone Lin (Macklin) reagent company.
Example 1
An insecticide comprising phosphatidylcholine.
The preparation method of the pesticide of the embodiment 1 comprises the following steps: a certain amount of phosphatidylcholine is weighed and directly dissolved in a proper amount of water to prepare phosphatidylcholine solutions with the concentration of 0.5-20 g/L respectively.
The application method comprises the following steps: a certain amount of pests are put on each plant leaf for feeding, and then phosphatidylcholine is sprayed on the leaf.
The application result shows that: the better the pest killing effect is with the increase of the concentration of the phosphatidylcholine solution, wherein 20g/L of the phosphatidylcholine solution has the best pest killing effect. Table 1 shows the statistical results of leaf pest numbers 24h after 20g/L phosphatidylcholine spraying.
Table 1: counting the number of leaf pests 24h after 20g/L phosphatidylcholine is sprayed
The results in table 1 show that: the average number of the bemisia tabaci, the myzus persicae, the frankliniella occidentalis and the rice planthoppers on the leaves is lower than 1 after the 20g/L phosphatidylcholine is sprayed for 24 hours, and the pest killing efficiency reaches 100 percent.
Example 2
An insecticide comprising phosphatidylinositol.
The preparation method of the pesticide of the embodiment 1 comprises the following steps: weighing a certain amount of phosphatidylinositol, and directly dissolving the phosphatidylinositol in a proper amount of water to prepare phosphatidylinositol solutions with the concentration of 1-20 g/L respectively.
The application method comprises the following steps: a certain amount of pests are put on each plant leaf for feeding, and phosphatidylinositol is sprayed on the leaf.
The application result shows that: the better the pest killing effect is with the increase of the concentration of the phosphatidylinositol solution, wherein 20g/L phosphatidylinositol solution has the best pest killing effect. Table 2 shows the statistical results of the leaf pest numbers 24h after 20g/L phosphatidylinositol spraying.
Table 2: counting the number of leaf pests 24h after 20g/L phosphatidylinositol is sprayed
Pest class Before spraying After spraying for 24 hours
Bemisia tabaci (head) 50 0.67±0.24
Peach aphid (head) 50 1.00±0.29
Frankliniella occidentalis (head) 50 1.33±0.33
Rice planthopper (head) 50 2.11±0.48
The results in Table 2 show that the average number of bemisia tabaci, myzus persicae, frankliniella occidentalis and rice planthoppers on the leaves is lower than 3 heads 24 hours after 20g/L phosphatidylinositol is sprayed, and the pest killing efficiency reaches 96%.
Example 3
An insecticide comprising phosphatidylethanolamine.
The preparation method of the pesticide of the embodiment 1 comprises the following steps: a certain amount of phosphatidylethanolamine is weighed and directly dissolved in a proper amount of water to prepare phosphatidylethanolamine solutions with the concentration of 1-20 g/L respectively.
The application method comprises the following steps: a certain amount of pests are put on each plant leaf for feeding, and then phosphatidylethanolamine is sprayed on the leaf.
The application result shows that: the better the pest killing effect is with the increase of the concentration of the phosphatidylethanolamine solution, wherein 20g/L of the phosphatidylethanolamine solution has the best pest killing effect. Table 3 shows the statistical results of the leaf pest numbers 24h after 20g/L phosphatidylethanolamine spraying.
Table 3: counting the number of leaf pests 24h after 20g/L phosphatidylethanolamine is sprayed
The results in Table 3 show that the average number of bemisia tabaci, myzus persicae, frankliniella occidentalis and rice planthoppers on the leaves is lower than 6 heads 24 hours after 20g/L phosphatidylethanolamine is sprayed, and the pest killing efficiency reaches 90%.
Example 4
An insecticide comprising phosphatidylcholine and phosphatidylinositol.
The preparation method of the pesticide of the embodiment 1 comprises the following steps: weighing a certain amount of phosphatidylcholine and phosphatidylinositol, and directly dissolving the phosphatidylcholine and the phosphatidylinositol in a proper amount of water to prepare a mixed solution of 1-10 g/L of phosphatidylcholine and 1-10 g/L of phosphatidylinositol.
The application method comprises the following steps: a certain amount of pests are put on each plant leaf for feeding, and then a mixed solution of 1-10 g/L phosphatidylcholine and 1-10 g/L phosphatidylinositol is sprayed on the leaf.
The application result shows that: the better the pest killing effect is with the increase of the concentration of the mixed solution of phosphatidylcholine and phosphatidylinositol, wherein the mixed solution of 10g/L phosphatidylcholine and 10g/L phosphatidylinositol has the best pest killing effect. Table 4 shows the statistical results of the number of leaf pests 24h after spraying 10g/L phosphatidylcholine and 10g/L phosphatidylinositol.
Table 4: counting the number of leaf pests 24h after 10g/L phosphatidylcholine and phosphatidylinositol are sprayed
Pest class Before spraying After spraying for 24 hours
Bemisia tabaci (head) 50 0±0
Peach aphid (head) 50 0.22±0.15
Frankliniella occidentalis (head) 50 0.89±0.31
Rice planthopper (head) 50 1.00±0.33
The results in Table 4 show that the average number of bemisia tabaci, myzus persicae, frankliniella occidentalis and rice planthoppers on the leaves is lower than 1 head 24 hours after 10g/L phosphatidylcholine and 10g/L phosphatidylinositol are sprayed, and the pest killing efficiency reaches 100%.
Example 5
An insecticide comprising phosphatidylcholine and phosphatidylethanolamine.
The preparation method of the pesticide of the embodiment 1 comprises the following steps: weighing a certain amount of phosphatidylcholine and phosphatidylethanolamine, and directly dissolving the phosphatidylcholine and the phosphatidylethanolamine in a proper amount of water to prepare a mixed solution of 1-10 g/L of phosphatidylcholine and 1-10 g/L of phosphatidylethanolamine.
The application method comprises the following steps: a certain amount of pests are put on each plant leaf for feeding, and then mixed solution of 1-10 g/L phosphatidylcholine and 1-10 g/L phosphatidylethanolamine is sprayed on the leaf.
The application result shows that: the better the pest killing effect is along with the increase of the concentration of the mixed solution of phosphatidylcholine and phosphatidylethanolamine, wherein 10g/L of phosphatidylcholine and 10g/L of phosphatidylethanolamine have the best pest killing effect. Table 5 shows the statistical results of the number of leaf pests 24h after spraying 10g/L phosphatidylcholine and 10g/L phosphatidylethanolamine.
Table 5: counting the number of leaf pests 24h after 10g/L of phosphatidylcholine and phosphatidylethanolamine are sprayed
Pest class Before spraying After spraying for 24 hours
Bemisia tabaci (head) 50 0±0
Peach aphid (head) 50 0.33±0.24
Frankliniella occidentalis (head) 50 1.11±0.35
Rice planthopper (head) 50 1.44±0.50
The results in Table 5 show that the average number of bemisia tabaci, myzus persicae, frankliniella occidentalis and rice planthoppers on the leaves is lower than 2 heads 24 hours after 10g/L phosphatidylcholine and 10g/L phosphatidylethanolamine are sprayed, and the pest killing rate reaches 98%.
Example 6
An insecticide comprising phosphatidylinositol and phosphatidylethanolamine.
The preparation method of the pesticide of the embodiment 1 comprises the following steps: weighing a certain amount of phosphatidylinositol and phosphatidylethanolamine, and directly dissolving the phosphatidylinositol and the phosphatidylethanolamine in a proper amount of water to prepare a mixed solution of 0.5-10 g/L phosphatidylinositol and 0.5-10 g/L phosphatidylethanolamine.
The application method comprises the following steps: a certain amount of pests are put on each plant leaf for feeding, and then 0.5-10 g/L of phosphatidylinositol and 0.5-10 g/L of phosphatidylethanolamine mixed solution are sprayed on the leaf.
The application result shows that: the better the pest killing effect is with the increase of the concentration of the mixed solution of phosphatidylinositol and phosphatidylethanolamine, wherein 10g/L phosphatidylinositol and 10g/L phosphatidylethanolamine have the best pest killing effect. Table 6 shows the statistical results of the number of leaf pests 24h after spraying 10g/L phosphatidylinositol and 10g/L phosphatidylethanolamine.
Table 6: counting the number of leaf pests 24h after 10g/L phosphatidylinositol and phosphatidylethanolamine are sprayed
Pest class Before spraying After spraying for 24 hours
Bemisia tabaci (head) 50 1.00±0.29
Peach aphid (head) 50 1.89±0.54
Frankliniella occidentalis (head) 50 2.56±0.60
Rice planthopper (head) 50 3.89±0.68
The results in Table 6 show that the average number of bemisia tabaci, myzus persicae, frankliniella occidentalis and rice planthoppers on the leaves is lower than 4 heads when 10g/L phosphatidylinositol and 10g/L phosphatidylethanolamine are sprayed for 24 hours, and the pest killing rate reaches 94%.
Example 7
An insecticide comprises phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine.
The preparation method of the pesticide of the embodiment 1 comprises the following steps: weighing a certain amount of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine, and directly dissolving the phosphatidylcholine, the phosphatidylinositol and the phosphatidylethanolamine in a proper amount of water to prepare a mixed solution of 0.1-10 g/L of phosphatidylcholine, 0.1-10 g/L of phosphatidylinositol and 0.1-10 g/L of phosphatidylethanolamine.
The application method comprises the following steps: a certain amount of pests are put on each plant leaf for feeding, and then 0.1-10 g/L of phosphatidylcholine, 0.1-10 g/L of phosphatidylinositol and 0.1-10 g/L of phosphatidylethanolamine are sprayed on the leaf.
The application result shows that: the better the pest killing effect is with the increase of the concentration of the mixed solution of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine, wherein 10g/L phosphatidylcholine, 10g/L phosphatidylinositol and 10g/L phosphatidylethanolamine have the best pest killing effect. Table 7 shows the statistical results of the number of leaf pests 24h after spraying 10g/L phosphatidylcholine, 10g/L phosphatidylinositol and 10g/L phosphatidylethanolamine.
Table 7: counting the number of leaf pests 24h after 10g/L phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine are sprayed
Pest class Before spraying After spraying for 24 hours
Bemisia tabaci (head) 50 0±0
Peach aphid (head) 50 0.33±0.17
Frankliniella occidentalis (head) 50 1.00±0.24
Rice planthopper (head) 50 1.11±0.26
The results in Table 7 show that the average number of the bemisia tabaci, the peach aphid, the frankliniella occidentalis and the rice planthopper on the leaf blade is lower than 2 when 10g/L phosphatidylcholine, 10g/L phosphatidylinositol and 10g/L phosphatidylethanolamine are sprayed for 24 hours, and the pest killing rate reaches 98%.
Experiment one: putting 50 bemisia tabaci on cotton leaves of each culture dish for feeding, spraying clear water, 20g/L phosphatidylcholine, 20g/L phosphatidylinositol and 20g/L phosphatidylethanolamine on the leaves respectively, and observing the number of the live bemisia tabaci on the leaves after 24 hours.
See fig. 1 for investigation results: after 24 hours, the number of live bemisia tabaci on the cotton leaves sprayed with the clean water is 48, and the number of live bemisia tabaci on the cotton leaves sprayed with the phosphatidylcholine, the phosphatidylinositol and the phosphatidylethanolamine is only 0, 1 and 3 respectively.
From the above results, it was revealed that a large number of deaths were observed after 24 hours by spraying any of phosphatidylcholine, phosphatidylinositol, and phosphatidylethanolamine. The three are combined at random, and the rapid insecticidal effect of the composition is also found.
The above description is only of the preferred embodiment of the present invention, and is not intended to limit the present invention in any way. While the invention has been described in terms of preferred embodiments, it is not intended to be limiting. Any person skilled in the art can make many possible variations and modifications to the technical solution of the present invention or equivalent embodiments using the method and technical solution disclosed above without departing from the spirit and technical solution of the present invention. Therefore, any simple modification, equivalent substitution, equivalent variation and modification of the above embodiments according to the technical substance of the present invention, which do not depart from the technical solution of the present invention, still fall within the scope of the technical solution of the present invention.

Claims (7)

1. Use of an insecticide in controlling pests, wherein the insecticide comprises one or more of phosphatidylcholine, phosphatidylinositol, and phosphatidylethanolamine, and the pest is one or more of bemisia tabaci, thrips, aphids, and brown planthoppers; the concentration of the phosphatidylcholine is 0.5-20 g/L, and the concentration of the phosphatidylinositol is 1-20 g/L; the concentration of the phosphatidylethanolamine is 1-20 g/L.
2. The use according to claim 1, wherein the insecticide comprises 1 to 10g/L phosphatidylcholine and 1 to 10g/L phosphatidylinositol.
3. The use according to claim 1, wherein the insecticide comprises 1 to 10g/L phosphatidylcholine and 1 to 10g/L phosphatidylethanolamine.
4. The use according to claim 1, wherein the insecticide comprises 0.5 to 10g/L phosphatidylinositol and 0.5 to 10g/L phosphatidylethanolamine.
5. The use according to claim 1, wherein the insecticide comprises 0.1 to 10g/L of phosphatidylcholine, 0.1 to 10g/L of phosphatidylinositol and 0.1 to 10g/L of phosphatidylethanolamine.
6. The use according to any one of claims 1 to 5, wherein the method of preparing the insecticide comprises the steps of: dissolving one or more of phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine in water to obtain the pesticide.
7. The use according to any one of claims 1 to 5, characterized in that the method of use is: spraying insecticide on the leaves of the plants, and killing the pests by feeding the pests on the leaves.
CN202210038640.6A 2022-01-13 2022-01-13 Insecticide and preparation method and application thereof Active CN114503998B (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0068297A1 (en) * 1981-06-27 1983-01-05 A. Nattermann & Cie. GmbH A new insecticidal composition, processes for its production and its use
EP0448070A1 (en) * 1990-03-21 1991-09-25 Ministero Dell' Universita' E Della Ricerca Scientifica E Tecnologica Insecticidal composition based on Bacillus thuringiensis
EP0579951A1 (en) * 1992-07-23 1994-01-26 Rikagaku Kenkyusho Pesticidal composition
CN102603790A (en) * 2012-02-21 2012-07-25 山东师范大学 Method for manufacturing high-purity phosphatidylcholine from Antarctic krill
CN110477003A (en) * 2019-08-09 2019-11-22 成都科利隆生化有限公司 A kind of acaricide containing soybean lecithin
CN113100251A (en) * 2021-04-25 2021-07-13 渭南东旺农华生物科技有限公司 Agricultural composition containing lecithin

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0068297A1 (en) * 1981-06-27 1983-01-05 A. Nattermann & Cie. GmbH A new insecticidal composition, processes for its production and its use
EP0448070A1 (en) * 1990-03-21 1991-09-25 Ministero Dell' Universita' E Della Ricerca Scientifica E Tecnologica Insecticidal composition based on Bacillus thuringiensis
EP0579951A1 (en) * 1992-07-23 1994-01-26 Rikagaku Kenkyusho Pesticidal composition
CN102603790A (en) * 2012-02-21 2012-07-25 山东师范大学 Method for manufacturing high-purity phosphatidylcholine from Antarctic krill
CN110477003A (en) * 2019-08-09 2019-11-22 成都科利隆生化有限公司 A kind of acaricide containing soybean lecithin
CN113100251A (en) * 2021-04-25 2021-07-13 渭南东旺农华生物科技有限公司 Agricultural composition containing lecithin

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Title
HPLC-ELSD法测定乳酸菌饮品中磷脂酰胆碱的质量浓度;窦文渊;王飞;石燕丽;巫培山;;中国乳品工业;41(02);第46页第1栏倒数第1段 *

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