CN114230019B - Application of probiotics activated sludge in sewage treatment - Google Patents
Application of probiotics activated sludge in sewage treatment Download PDFInfo
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- CN114230019B CN114230019B CN202111243321.0A CN202111243321A CN114230019B CN 114230019 B CN114230019 B CN 114230019B CN 202111243321 A CN202111243321 A CN 202111243321A CN 114230019 B CN114230019 B CN 114230019B
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- 239000010802 sludge Substances 0.000 title claims abstract description 40
- 239000006041 probiotic Substances 0.000 title claims abstract description 37
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 37
- 239000010865 sewage Substances 0.000 title claims abstract description 30
- 239000007787 solid Substances 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 14
- 230000000529 probiotic effect Effects 0.000 claims abstract description 13
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 239000002609 medium Substances 0.000 claims abstract description 8
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- 239000012530 fluid Substances 0.000 claims abstract description 5
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 3
- 238000005273 aeration Methods 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000010902 straw Substances 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 238000003501 co-culture Methods 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 3
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 3
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000006188 syrup Substances 0.000 claims description 3
- 235000020357 syrup Nutrition 0.000 claims description 3
- 239000005696 Diammonium phosphate Substances 0.000 claims description 2
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 2
- 241000235342 Saccharomycetes Species 0.000 claims description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 2
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 2
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- 239000004202 carbamide Substances 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 2
- 239000006012 monoammonium phosphate Substances 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 2
- 150000002772 monosaccharides Chemical class 0.000 claims description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- 230000000243 photosynthetic effect Effects 0.000 claims description 2
- 244000005700 microbiome Species 0.000 abstract description 23
- 238000000034 method Methods 0.000 abstract description 9
- 239000000126 substance Substances 0.000 abstract description 5
- 239000002689 soil Substances 0.000 abstract description 4
- 239000002154 agricultural waste Substances 0.000 abstract description 2
- 238000000465 moulding Methods 0.000 abstract description 2
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 238000005189 flocculation Methods 0.000 description 3
- 230000016615 flocculation Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000009264 composting Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000003311 flocculating effect Effects 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028813 Nausea Diseases 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000008394 flocculating agent Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000002340 glycosyl compounds Chemical class 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000008693 nausea Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses application of probiotic activated sludge in sewage treatment. The method comprises the following specific steps: adding probiotics and water into a solid culture medium, performing solid fermentation at 25-35 ℃ and constant temperature and humidity of relative humidity 60-90% RH, and molding to obtain a solid fermentation preparation; adding the solid fermentation preparation into a nutrient medium for expansion culture; adding the expanded liquid into sewage with the same volume, and then adding a water treatment microbial inoculum for co-cultivation to obtain mixed liquid; transferring the mixed liquid into sewage, culturing in a fluid state until the sewage reaches the discharge standard, and performing solid-liquid separation to form the probiotic activated sludge, wherein the input amount of the mixed liquid is set according to the actual scale. The application takes probiotics as a main body, and harmful microorganisms are far lower than the traditional activated sludge, and even if the harmful microorganisms enter soil, agricultural wastes and other substances, the harmful microorganisms can play a role in probiotics. In general, the invention realizes the modification treatment of biological sludge while purifying sewage.
Description
Technical Field
The invention relates to the technical field of microorganism culture and sewage treatment, in particular to application of probiotics activated sludge in sewage treatment.
Background
Activated sludge is a treatment method commonly used in water treatment, and is essentially a microbial population which is formed under natural conditions and consumes pollutants in water and organic matters and inorganic matters attached to the microbial population. The water contains a kind of microorganisms capable of generating flocculating agents, and the microorganisms integrate other microorganisms without flocculation characteristics, organic matters and inorganic matters in the water body, and on the basis of the microorganisms, the propagation of living bacteria and the degradation of dead bacteria simultaneously occur, so that the living bacteria and the degradation of dead bacteria are changed into a sludge-like substance, and the sludge-like substance has certain water treatment activity and is called activated sludge.
The microorganisms in activated sludge are very complex, and are propagated by natural microorganisms in a water body and other microorganisms in the environment, so that the activated sludge does not have microbial safety from the viewpoint of safety. In particular, harmful microorganisms from feces in municipal sewage can also become a major component in activated sludge.
The microorganisms are not limited in the treatment of the activated sludge, and the factors are not considered in the treatment of the activated sludge, whether the activated sludge is recycled or buried. During landfill, harmful microorganisms can permeate from the sludge with the permeate, and contaminate the soil and even equipment as it passes through the water stream. In a strict composting process, harmful microorganisms may generate nausea odor to pollute the environment, and even under high temperature decomposition, 1-2% of harmful microorganisms remain, and along with the fertilizer, the fertilizer may be capable of being caused by plant diseases and the like.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the application of the probiotics activated sludge in sewage treatment, the activated sludge has high environmental friendliness and good safety, and the activated sludge used for sewage treatment can be used as a raw material for recycling and can be used for multiple directions of fertilizer production, soil restoration and black and odorous water body treatment.
Aiming at the defects of the prior art, the invention adopts the following technical scheme:
an application of probiotic activated sludge in sewage treatment, comprising the following steps:
step 1, adding probiotics and water into a solid culture medium, performing solid fermentation at a constant temperature and a constant humidity of between 25 and 35 ℃ and a relative humidity of between 60 and 90 percent RH, and forming to obtain a solid fermentation preparation;
step 2, adding the solid fermentation preparation into a nutrient medium for expansion culture;
step 3, adding the liquid after the expansion culture into the sewage with the same volume, and then adding the water treatment microbial inoculum for co-culture to obtain mixed liquid;
and 4, transferring the mixed liquid into sewage, culturing in a fluid state until the sewage reaches the discharge standard, and performing solid-liquid separation to form the probiotic activated sludge, wherein the input amount of the mixed liquid is set according to the actual scale.
As an improvement, the probiotics in the step 1 are compound flora, and the compound flora comprises at least 2 of saccharomycetes, lactic acid bacteria, bacillus and photosynthetic flora; the water content of the solid culture medium is 35-75% by mass fraction, the addition amount of the probiotic bacteria agent is 0.1-1% by mass fraction, and the solid fermentation time is 24-48h.
As an improvement, the solid medium in step 1 comprises the following components in weight fraction: 10-50% of corn flour, 10-50% of wine groove and 10-20% of soybean meal.
Further improved is that the solid culture medium in the step 1 also comprises one or two of bran or straw powder; the weight fraction of the bran is not more than 20%, and the weight fraction of the straw powder is not more than 10%.
As an improvement, the nutrient medium in step 2 comprises the following components: starch 0-10g/L, syrup 0-10g/L, monosaccharide 0-10g/L, protein powder 0-2g/L, urea 0-1g/L, diammonium phosphate 0-1g/L, monoammonium phosphate 0-1g/L, potassium dihydrogen phosphate 0-0.1g/L, dipotassium hydrogen phosphate 0-0.1g/L, and ammonium sulfate 0-1g/L; the solid state fermentation preparation for the expansion culture has the addition amount of 1-10g/L, the expansion culture time of 0.5-2 days, the culture temperature of 20-40 ℃ and the aeration rate of 0-100 times CV, wherein CV means that one unit of liquid volume flows per minute, the aeration rate is 0CV which usually takes the added liquid amount as a standard, and aeration can be omitted.
As an improvement, the mixed liquid used in the step 4 is added in an amount of 1kg of the solid fermentation preparation, calculated as the solid fermentation preparation, which can be used for a sewage treatment scale of 20-200 kg/d.
As an improvement, the fluid culture in the step 4 is carried out under the condition that the inflow water and the outflow water are kept stable, the culture environment is 0-30 ℃, the aeration rate is 0-100 times CV, and the aeration rate is 0CV, which means that aeration can be omitted.
Further improved is that the aeration in the culture process is staged aeration.
The beneficial effects are that:
compared with the prior art, the application of the probiotics activated sludge in sewage treatment has the other purpose of modifying biological sludge except sewage purification. The traditional discharged activated sludge contains viruses and harmful microorganisms, even the microorganisms can be greatly propagated and have biological dangers, so that the safety coefficient of the biochemical sludge is lower in the composting application process, and even in the stacking and storage process, the biochemical dangers are generated. The probiotic activated sludge has a microorganism composition of probiotics, has very good microorganism safety, and harmful microorganisms are far lower than those of the traditional activated sludge, and can play a probiotic role even if entering into soil, agricultural wastes and other substances. It is safer to discharge, store, and even utilize, and generally, the present invention changes the properties of biochemical sludge. Has the following advantages:
1. the microorganisms used in the invention are all probiotics, and the probiotics comprise the effects of promoting nutrient absorption and growth of animals and plants, restoring the environment, inhibiting harmful bacteria and the like;
2. the probiotics have flocculation capability, and the core of the probiotics is glycoprotein, lipoprotein, glycosyl compound and the like with charges, and the effect of flocculating small molecular substances is realized through positive and negative charges, so that the probiotics flocs can be formed under the condition of not depending on flocculating microorganisms in the environment, and further active sludge particles are formed with other probiotics;
3. the active sludge probiotics in the invention has rich content, can be used as probiotics microbial inoculant to directly act on a plurality of fields of agriculture, environmental protection and the like.
Drawings
FIG. 1 shows the suspension loading of different activated sludge, wherein (a) is common activated sludge and (b) is the probiotic activated sludge according to the invention.
Detailed Description
Example 1
A method for culturing probiotic activated sludge, comprising the following steps:
1) Adding 10g of probiotics (biological flocculation bag, water environment restoration series of Dalian liter Tai aquatic feed Co., ltd.) into 1kg of solid culture medium (the solid culture medium is 48% of wine tank, 16% of bean pulp, 15% of bran, 11% of corn flour, 10% of straw powder and 62% of water content), culturing at 30deg.C for 30h, and molding to obtain solid fermentation preparation;
2) 1kg of solid fermentation preparation is put into 100L of nutrient medium, the culture time is 2 days at the temperature of 30 ℃, the aeration rate is 3 CV in the culture process, and the components of the used nutrient medium are 8g/L of starch, 6g/L of syrup, 10g/L of glucose, 2g/L of protein powder and 0.05g/L of diammonium hydrogen phosphate;
3) Adding 100L of domestic sewage into the culture solution, adding 0.4kg of bacillus fungicide (purchased from the national environmental protection and technology Co., ltd., aerobic special fungicide series) into the culture solution, and culturing at 30 ℃ for 3 days, wherein aeration amount is 2 CV during the culturing process to obtain mixed liquid;
4) All the mixed liquid is transferred into an activated sludge aerobic tank with the treatment capacity of 1t/d for treatment, the aeration capacity is 2 CV, the water quality is detected after the culture is finished, and the water quality conditions before and after the treatment are as follows:
TABLE 1 Table of the conditions before and after sewage treatment
The treated water quality reaches the rural domestic sewage discharge standard.
In the foregoing, the protection scope of the present invention is not limited to the preferred embodiments of the present invention, and any simple changes or equivalent substitutions of the technical solutions that can be obviously obtained by those skilled in the art within the technical scope of the present invention disclosed in the present invention fall within the protection scope of the present invention.
Claims (4)
1. The application of the probiotics activated sludge in sewage treatment is characterized by comprising the following steps:
step 1, adding probiotics and water into a solid culture medium, performing solid fermentation at a constant temperature and humidity of between 25 and 35 ℃ and a relative humidity of between 60 and 90 percent RH, and forming to obtain a solid fermentation preparation, wherein the probiotics are compound flora, and the compound flora comprises at least 2 of saccharomycetes, lactic acid bacteria, bacillus and photosynthetic flora; the water content of the solid culture medium is 35-75% by mass, the addition amount of the probiotics is 0.1-1% by mass, the solid fermentation time is 24-48h, and the solid culture medium comprises the following components by weight: 10-50% of corn flour, 10-50% of wine tank, 10-20% of bean pulp, and one or two of bran and straw powder; the weight fraction of the bran is not more than 20%, and the weight fraction of the straw powder is not more than 10%;
step 2, adding the solid fermentation preparation into a nutrient medium for expansion culture, wherein the nutrient medium comprises the following components: starch 0-10g/L, syrup 0-10g/L, monosaccharide 0-10g/L, protein powder 0-2g/L, urea 0-1g/L, diammonium phosphate 0-1g/L, monoammonium phosphate 0-1g/L, potassium dihydrogen phosphate 0-0.1g/L, dipotassium hydrogen phosphate 0-0.1g/L, and ammonium sulfate 0-1g/L; the addition amount of the solid fermentation preparation for the expansion culture is 1-10g/L, the time of the expansion culture is 0.5-2 days, the culture temperature is 20-40 ℃, and the aeration rate is 0-100 times CV, wherein CV means that one unit of liquid volume flows per minute, and the added liquid amount is taken as a standard;
step 3, adding the liquid after the expansion culture into the sewage with the same volume, and then adding a water treatment microbial inoculum to perform co-culture to obtain a mixed liquid, wherein the water treatment microbial inoculum is a spore type aerobic microbial inoculum, the addition amount is 0.01-1%, the co-culture time is 0.5-3 days, the culture temperature is 20-40 ℃, and the aeration rate is 0.1-100 times CV;
and 4, transferring the mixed liquid into sewage, culturing in a fluid state until the sewage reaches the discharge standard, and performing solid-liquid separation to form the probiotic activated sludge, wherein the input amount of the mixed liquid is set according to the actual scale.
2. The use of a probiotic activated sludge in sewage treatment according to claim 1, characterized in that the amount of mixed liquid used in step 4 is 1kg of solid fermentation preparation, calculated as solid fermentation preparation, for sewage treatment scale of 20-200 kg/d.
3. The use of a probiotic activated sludge in sewage treatment according to claim 1, wherein the fluid culture in step 4 is performed under the condition that inflow water and outflow water are kept stable, and the culture environment is room temperature, and the aeration is 0-100 times CV.
4. Use of a probiotic activated sludge in the treatment of sewage according to claim 3, characterized in that the aeration during the cultivation is a staged aeration.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111243321.0A CN114230019B (en) | 2021-10-25 | 2021-10-25 | Application of probiotics activated sludge in sewage treatment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111243321.0A CN114230019B (en) | 2021-10-25 | 2021-10-25 | Application of probiotics activated sludge in sewage treatment |
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| CN114230019B true CN114230019B (en) | 2023-07-25 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1348987A (en) * | 2001-12-02 | 2002-05-15 | 重庆和润实业(集团)有限公司 | Prepn of microbial colony sludge for efficient treatment of sewage |
| CN1257113C (en) * | 2003-10-08 | 2006-05-24 | 上海创博生态工程有限公司 | Microbiological ammonia nitrogen aligning agent and method for making the same |
| CN101899397B (en) * | 2010-07-06 | 2012-12-05 | 北京三泰正方生物环境科技发展有限公司 | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application |
| CN105462880A (en) * | 2015-12-10 | 2016-04-06 | 南平正大欧瑞信生物科技开发有限公司 | Preparation method of sewage processing complex bacterium |
| CN108017161A (en) * | 2017-12-25 | 2018-05-11 | 陈益德 | A kind of superior microorganism solid fungicide preparation method for creek water pollution control |
| RU2721534C1 (en) * | 2019-05-22 | 2020-05-19 | Вячеслав Сергеевич Поляхов | Method of water treatment for cultivation of hydrobionts in closed volumes and device implementing thereof |
| CN110438059A (en) * | 2019-08-01 | 2019-11-12 | 上海环境卫生工程设计院有限公司 | A kind of superior microorganism microbial inoculum of water pollution control and preparation method thereof |
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