CN108017161A - Preparation method of dominant microorganism solid microbial inoculum for river water pollution treatment - Google Patents

Abstract

The invention belongs to the technical field of pollution treatment, and particularly relates to a preparation method of a dominant microorganism solid microbial inoculum for river water pollution treatment, which comprises the following specific preparation steps: s1: preparing a culture medium; s2: culturing the microorganism; s3: injecting muddy water polluted by the river channel, and selecting strains in a targeted manner; s4: the preparation method of the dominant microorganism solid microbial inoculum for treating the river water pollution has the advantages that the river pollution is treated by the strains, the biological method is green and environment-friendly, the river pollution can be treated for a long time, and the solid microbial inoculum is convenient to store and transport by a cold storage mode.

Description

A preparation method of dominant microbial solid bacterial agent for river water pollution control

technical field

The invention relates to the technical field of pollution control, in particular to a method for preparing a dominant microbial solid bacterial agent for river water pollution control.

Background technique

With the development of social economy and the improvement of people's living standards, the dense population brings domestic sewage, and the spread of small factories brings industrial wastewater. These untreated domestic sewage, untreated or untreated industrial production wastewater are also discharged with When the organic pollutants exceed the limit of natural purification that the river water body can hold, the dissolved oxygen in the water will be consumed rapidly and become anoxic, and the river water body will become eutrophic. The water body turns black and smelly. Due to the lack of oxygen, the survival of the original ecological organisms is directly threatened, until death, corpse decay, further pollution of the water body and stench, such a vicious cycle continues, completely destroying the ecology of the river.

At present, the river surge control methods mainly include closure, dredging, running water circulation (that is, water diversion to increase flow), artificial aeration of river channels, sediment covering, coagulation, ecological restoration and other methods. Although these methods have alleviated the problem of the deterioration of the river gushing water environment to a certain extent, there are many shortcomings.

Contents of the invention

The object of the present invention is to provide a kind of preponderant microbial solid bacterial agent preparation method that is used for river water pollution control, to solve the present problem that proposes in the above-mentioned background technology, river surge treatment method mainly contains interception, dredging, running water circulation (being water diversion to increase flow) ), artificial aeration of river channels, sediment covering, coagulation, ecological restoration and other methods. Although these methods have alleviated the problem of the deterioration of the river gushing water environment to a certain extent, there are many deficiencies.

In order to achieve the above object, the present invention provides the following technical solutions: a method for preparing a solid microbial agent for river water pollution control, the specific preparation steps of the solid microbial agent for river water pollution control are as follows:

S1: Culture medium preparation: Take 500g of beef, soak the obtained 500g of beef in 800ml-850ml of water, keep the soaked beef at 20-25 degrees Celsius for 8-10 hours, filter the soaked meat For gravy, add 300g of peptone and 4-5g of NaCl per 100ml of the gravy, heat and mix the gravy with peptone and sodium chloride until the gravy is concentrated into a thick state, add soda and continue stirring, and check the pH value of the gravy at 7.0-7.2 That is, the PH-adjusted medium base material is sterilized by high-temperature steam, and 2.2g-2.4g of agar is added to the sterilized medium base material per 100ml, and mixed evenly;

S2: Cultivate microorganisms: uniformly inoculate different types of strains on the culture medium prepared in step S1, place the culture medium after the inoculated strains at a temperature of 25-30 degrees Celsius for cultivation, and the number of days for cultivation is 15 days. Colonies grow evenly on the culture medium, add a pH regulator every 3 days during the cultivation process, and measure the pH when adding the pH regulator K ₂ HPO ₄ until the pH value returns to 7.0-7.2 again;

S3: Inject the muddy water polluted by the river, and select the bacteria in a targeted manner: select the mud at the bottom of the river and the river water within 20cm from the bottom of the ground from the river section that needs to be treated, mix the taken out river water with the river mud, and mix evenly Pour the muddy water into the medium containing the colonies in step S2, add 30-35ml of muddy water into the 150ml medium, continue to cultivate the medium injected with muddy water for 3-5 days, observe and take out the dead bacterium colonies during the cultivation, and cultivate At 8-10 days, the living bacterial colonies after cultivation were taken out;

S4: Proliferation of microorganisms: Store the remaining muddy water in equal parts according to the amount of 100ml, add the living colonies taken out in step S3 to the muddy water, and add 4-5 groups of colonies per 100ml, and disperse and cultivate the colonies for 3- After 5 days, add the cultivated mud water to the bread, put the cotton bag in the mud water and soak for 2-3 hours, the colony finds nutrients and then adsorbs on the bread, take out the bread to remove the colonies adsorbed on the bread, The colony is refrigerated by cooling down, and the colony can be revived by taking out the cotton bag that has adsorbed the colony and warming it up.

Preferably, an antifoaming additive is also added during the preparation of the medium in the step S1.

Preferably, the defoaming additive is emulsified silicone oil.

Preferably, the soaking temperature of the beef in the step S1 is 25 degrees Celsius, and the soaking time is 9.5 hours.

Preferably, the pH regulator is K ₂ HPO ₄ or KH ₂ PO ₄ .

Preferably, the temperature for refrigerating the colony in the step S4 is -5°C-0°C.

Compared with the prior art, the beneficial effects of the present invention are: the preparation method of the advantageous microbial solid bacterial agent used for the treatment of river water pollution can control the river pollution by the bacteria, and the biological method is green and environmentally friendly, and can prevent the river pollution for a long time. For treatment, through the refrigerated storage method, the solid bacterial agent is conveniently stored and transported.

Description of drawings

Figure 1 is a flow chart of the preparation process of the present invention.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Please refer to Fig. 1, the present invention provides a kind of technical scheme: a kind of method for preparing the solid bacterial agent of dominant microorganisms used in the treatment of river water pollution, the specific preparation steps of the method for preparing the solid microbial agent of dominant microorganisms for the treatment of river water pollution are as follows :

S1: Culture medium preparation: Take 500g of beef, soak the obtained 500g of beef in 800ml-850ml of water, keep the soaked beef at 20-25 degrees Celsius for 8-10 hours, filter the soaked meat For gravy, add 300g of peptone and 4-5g of NaCl per 100ml of the gravy, heat and mix the gravy with peptone and sodium chloride until the gravy is concentrated into a thick state, add soda and continue stirring, and check the pH value of the gravy at 7.0-7.2 That is, the PH-adjusted medium base material is sterilized by high-temperature steam, and 2.2g-2.4g of agar is added to the sterilized medium base material per 100ml, and mixed evenly;

S2: Cultivate microorganisms: evenly inoculate different types of strains on the culture medium prepared in step S1, place the culture medium after inoculating the strains at a temperature of 25-30 degrees Celsius for cultivation, and the number of days for cultivation is 15 days. Colonies grow evenly on the culture medium, add a pH regulator every 3 days during the cultivation process, and measure the pH when adding the pH regulator K ₂ HPO ₄ until the pH value returns to 7.0-7.2 again;

S3: Inject the muddy water polluted by the river, and select the bacteria in a targeted manner: select the mud at the bottom of the river and the river water within 20cm from the bottom of the ground from the river section that needs to be treated, mix the taken out river water with the river mud, and mix evenly Pour the muddy water into the medium containing the colonies in step S2, add 30-35ml of muddy water into the 150ml medium, continue to cultivate the medium injected with muddy water for 3-5 days, observe and take out the dead bacterium colonies during the cultivation, and cultivate At 8-10 days, the living bacterial colonies after cultivation were taken out;

S4: Proliferation of microorganisms: Store the remaining muddy water in equal parts according to the amount of 100ml, add the living colonies taken out in step S3 to the muddy water, and add 4-5 groups of colonies per 100ml, and disperse and cultivate the colonies for 3- After 5 days, add the cultivated mud water to the bread, put the cotton bag in the mud water and soak for 2-3 hours, the colony finds nutrients and then adsorbs on the bread, take out the bread to remove the colonies adsorbed on the bread, The colony is refrigerated by cooling down, and the colony can be revived by taking out the cotton bag that has adsorbed the colony and warming it up.

Wherein, during the preparation of the culture medium in step S1, an antifoaming additive is also added, and the antifoaming additive is emulsified silicone oil. In step S1, the soaking temperature of the beef is 25 degrees Celsius, the soaking time is 9.5 hours, and the pH regulator is K ₂ HPO ₄ or KH ₂ PO ₄ , the temperature of the refrigerated colony in step S4 is -5°C-0°C.

Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes, modifications and substitutions can be made to these embodiments without departing from the principle and spirit of the present invention. and modifications, the scope of the invention is defined by the appended claims and their equivalents.

Claims (6)

1. A method for preparing a dominant microbial solid bacterial agent for river water pollution control, characterized in that: the specific preparation steps of the dominant microbial solid bacterial agent preparation method for river water pollution control are as follows: S1: Culture medium preparation: take 500g of beef, soak the obtained 500g of beef in 800ml-850ml of water, keep soaking the soaked beef for 8-10 hours at a temperature of 20-25 degrees Celsius, and filter the soaked meat For gravy, add 300g of peptone and 4-5g of NaCl per 100ml of the gravy, heat and mix the gravy with peptone and sodium chloride until the gravy is concentrated into a thick state, add soda and continue stirring, and check the pH value of the gravy at 7.0-7.2 That is, the PH-adjusted medium base material is sterilized by high-temperature steam, and 2.2g-2.4g of agar is added to the sterilized medium base material per 100ml, and mixed evenly; S2: Cultivate microorganisms: uniformly inoculate different types of strains on the culture medium prepared in step S1, place the culture medium after the inoculated strains at a temperature of 25-30 degrees Celsius for cultivation, and the number of days for cultivation is 15 days. Colonies grow evenly on the culture medium, add a pH regulator every 3 days during the cultivation process, and measure the pH when adding the pH regulator K ₂ HPO ₄ until the pH value returns to 7.0-7.2 again; S3: Inject the muddy water polluted by the river, and select the bacteria in a targeted manner: select the mud at the bottom of the river and the river water within 20cm from the bottom of the ground from the river section that needs to be treated, mix the taken out river water with the river mud, and mix evenly Pour the muddy water into the medium containing the colonies in step S2, add 30-35ml of muddy water into the 150ml medium, continue to cultivate the medium injected with muddy water for 3-5 days, observe and take out the dead bacterium colonies during the cultivation, and cultivate At 8-10 days, the living bacterial colonies after cultivation were taken out; S4: Proliferation of microorganisms: Store the remaining muddy water in equal parts according to the amount of 100ml, add the living colonies taken out in step S3 to the muddy water, and add 4-5 groups of colonies per 100ml, and disperse and cultivate the colonies for 3- After 5 days, add the cultivated mud water to the bread, put the cotton bag in the mud water and soak for 2-3 hours, the colony finds nutrients and then adsorbs on the bread, take out the bread to remove the colonies adsorbed on the bread, The colony is refrigerated by cooling down, and the colony can be revived by taking out the cotton bag that has adsorbed the colony and warming it up. 2. A method for preparing a dominant microbial solid bacterial agent for river water pollution control according to claim 1, characterized in that an antifoaming additive is also added during the preparation of the medium in the step S1. 3. A method for preparing a dominant microbial solid bacterial agent for river water pollution control according to claim 2, characterized in that: the defoaming additive is emulsified silicone oil. 4. A method for preparing a solid bacterial agent of dominant microorganisms for river water pollution control according to claim 1, characterized in that: in the step S1, the soaking temperature of the beef is 25 degrees Celsius, and the soaking time is 9.5 hours. 5 . The method for preparing a dominant microbial solid bacterial agent for river water pollution control according to claim 1 , wherein the pH regulator is K ₂ HPO ₄ or KH ₂ PO ₄ . 6 . A method for preparing a dominant microbial solid bacterial agent for river water pollution control according to claim 1 , characterized in that: the temperature for refrigerating the colony in the step S4 is -5°C-0°C.