CN102942417A - Plant growth promoting rhinoacteria (PGPR) bio-organic fertilizers and production method thereof - Google Patents
Plant growth promoting rhinoacteria (PGPR) bio-organic fertilizers and production method thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses plant growth promoting rhinoacteria (PGPR) bio-organic fertilizers which are produced by the following steps of evenly mixing pig manure subjected to anaerobic fermentation with stevia slag subjected to anaerobic fermentation to obtain mixed materials, stacking the mixed materials to perform fermentation, adding leavening agents into the mixed materials when the mixed materials are stacked to perform fermentation, keeping the temperature of the stacked materials to range from 54 DEG C to 70 DEG C, performing the fermentation for 7-10 days, mixing the fermented materials with humic acid and inorganic nutrients, smashing the mixture, adding PGPR into the powder, and evenly mixing to obtain the PGPR bio-organic fertilizers. The PGPR bio-organic fertilizers are produced by taking the residual stevia slag of the sugar making enterprises and pig manure which is breeding waste of pig-breeding enterprises as the main raw materials. The produced PGPR bio-organic fertilizers are high in content of organic matter and strong in biological activity, and have disease resisting and production increasing effect.
Description
Technical field
The present invention relates to technical field of fertilizers, be specifically related to a kind of PGPR biological organic fertilizer, also relate to simultaneously its production method.
Background technology
The PGPR biological organic fertilizer refers to microorganisms with specific functions---plant growth-promoting rhizobacteria (PGPR) with mainly take plant and animal residues (such as feces of livestock and poultry, agricultural crop straw etc.) for originating and having the fertilizer of microbial fertilizer and fertilizer effect through the class that harmless treatment, the organic materials that becomes thoroughly decomposed are composited concurrently.
In recent years, biological organic fertilizer production and production technology research and development thereof become the focus that everybody pays close attention to increasingly, and the production technique of having reported at present is also more, and weak point mainly is that raw materials for production are relatively more expensive, and cost is high, and the production cycle is longer.
Summary of the invention
The purpose of this invention is to provide a kind of PGPR biological organic fertilizer.
The present invention also aims to provide a kind of production method of PGPR biological organic fertilizer.
In order to realize above purpose, the technical solution adopted in the present invention is: a kind of PGPR biological organic fertilizer makes by the production method that may further comprise the steps:
(1) preparation mixture
To mix through the pig manure of anaerobically fermenting with through the stevia rebaudiana dregs of anaerobically fermenting, and mix thoroughly, and make mixture, the weight percentage through the pig manure of anaerobically fermenting in the described mixture is 35-45%;
(2) mixture stacking fermentation
Described mixture carries out the stacking fermentation, in described mixture, add starter during the stacking fermentation, the addition of starter is the 1-5 ‰ of described mixture weight, the mixture clamp method is: lay one deck mixture, then on the mixture bed of material, spread the described starter of one deck, sprinkle water on mixture during stacking, the weight percentage of water is controlled to be 55-65% in the mixture, the complete stockpile that obtains of mixture stacking; During described stockpile temperature 〉=54 ℃, stockpile is carried out turning, and keep the skin wet, make that the weight percentage of water remains 55-65% in the stockpile, the stockpile temperature remains 54-70 ℃ afterwards, ferments 7-10 days, turning is 2-3 time between yeast phase, it is complete to ferment, and makes fermentation materials, and the weight percentage of water is 20-30% in the described fermentation materials;
(3) preparation powder
Described fermentation materials is mixed with humic acids, inorganic nutrients, pulverize, make powder, the weight percentage of fermentation materials is 80-85% in the described powder, and the weight percentage of described humic acids is 13-18%, and the weight percentage of inorganic nutrients is 1-2%;
(4) preparation PGPR biological organic fertilizer
Preparation powdery PGPR biological organic fertilizer: add the PGPR bacterium in described powder, the addition of described PGPR bacterium is the 1-2 ‰ of described powder weight, stirs, and makes the PGPR biological organic fertilizer;
Preparation particulate state PGPR biological organic fertilizer: with described granulation for powders, oven dry, cooling, sub-sieve, make granulated fertilizer, by the mode of dressing that PGPR bacterium bag is surperficial to described granulated fertilizer again, the addition of described PGPR bacterium is the 1-2 ‰ of described powder weight, makes particulate state PGPR biological organic fertilizer.
The C/N value of the mixture that step (1) makes is 23-25, and the pH value is 6-8.
Described starter is aerobic thermophilic zymophyte.
Described aerobic thermophilic zymophyte is colloid bacillus cereus and/or subtilis.
The addition of described starter is 3 ‰ of described mixture weight.
The weight percentage of water is controlled to be 60% in the middle mixture of step (2).
The weight percentage of water is 25% in the described fermentation materials.
Inorganic nutrients wherein refers to nitrogen-phosphorus-potassium compound fertilizer, and its effect is the total nutrient of regulating the PGPR biological organic fertilizer.
Because the C/N value of stevia rebaudiana dregs is relatively high, the C/N value of pig manure is relatively low, therefore during the mixture in preparation process (1), stevia rebaudiana dregs and pig manure should mix according to suitable ratio, in the mixture of the present invention through the preferred 35-45% of weight percentage of the pig manure of anaerobically fermenting.
A kind of production method of PGPR biological organic fertilizer may further comprise the steps:
(1) preparation mixture
To mix through the pig manure of anaerobically fermenting with through the stevia rebaudiana dregs of anaerobically fermenting, and mix thoroughly, and make mixture, the weight percentage through the pig manure of anaerobically fermenting in the described mixture is 35-45%;
(2) mixture stacking fermentation
Described mixture carries out the stacking fermentation, in described mixture, add starter during the stacking fermentation, the addition of starter is the 1-5 ‰ of described mixture weight, the mixture clamp method is: lay one deck mixture, then on the mixture bed of material, spread the described starter of one deck, sprinkle water on mixture during stacking, the weight percentage of water is controlled to be 55-65% in the mixture, the complete stockpile that obtains of mixture stacking; When described stockpile temperature reaches more than 54 ℃, stockpile is carried out turning, and keep the skin wet, make that the weight percentage of water remains 55-65% in the stockpile, the stockpile temperature remains 54-70 ℃ afterwards, ferments 7-10 days, turning is 2-3 time between yeast phase, it is complete to ferment, and makes fermentation materials, and the weight percentage of water is 20-30% in the described fermentation materials;
(3) preparation powder
Described fermentation materials is mixed with humic acids, inorganic nutrients, pulverize, make powder, the weight percentage of fermentation materials is 80-85% in the described powder, and the weight percentage of described humic acids is 13-18%, and the weight percentage of inorganic nutrients is 1-2%;
(4) preparation PGPR biological organic fertilizer
Preparation powdery PGPR biological organic fertilizer: add the PGPR bacterium in described powder, the addition of described PGPR bacterium is the 1-2 ‰ of described powder weight, stirs, and makes the PGPR biological organic fertilizer;
Preparation particulate state PGPR biological organic fertilizer: with described granulation for powders, oven dry, cooling, sub-sieve, make granulated fertilizer, by the mode of dressing that PGPR bacterium bag is surperficial to described granulated fertilizer again, the addition of described PGPR bacterium is the 1-2 ‰ of described powder weight, makes particulate state PGPR biological organic fertilizer.
PGPR biological organic fertilizer provided by the invention, to make take the breeding waste pig manure of the stevia rebaudiana dregs of the remnants of sugar enterprise and pig-breeding enterprise as main material production, the PGPR biological organic fertilizer organic content that makes is high, and biological activity is strong, has disease-resistant, effect of increasing production.
At present, most of sweet Stevia processing enterprise and pig-breeding enterprise all are faced with the problem that industrial residue and breeding waste can't be processed and discharge.The raw material refuse reclamation is for such enterprise, complex process not only, cost is also high, thereby in order to save cost, most enterprises has been taked comparatively simple treatment process, directly stevia rebaudiana dregs or pig manure are produced biogas with waste water through anaerobically fermenting, and remaining cripple major part is landfilled, even discarded, seldom be utilized, can't fundamentally solve the environment protection treating problem, bring larger inconvenience to urban environment and resident living, and cause great pressure also for local efforts at environmental protection.PGPR method for producing biological organic fertilizer provided by the invention, solved as how stevia rebaudiana dregs and pig manure are that raw material production goes out low cost, and meet industry standard the biological organic fertilizer product, solve sweet Stevia processing enterprise's waste residue and breeding enterprise offal treatment and deposit, slow down the series of problems such as environmental administration's pressure, the advantages such as it is low to have production cost, and fermentation period is short.Stevia rebaudiana dregs contains the nutrition such as a large amount of carbohydrates, fat, organic matter and nitrogen phosphorus potassium, can satisfy aerobic thermophilic zymophyte breeding and amplification to the demand of nutrition; Take stack type fermentation pattern fermentation materials, be beneficial to ventilatedly, can satisfy the aerobic biological nature of aerobic thermophilic zymophyte, stevia rebaudiana dregs and pig manure mixture can thoroughly, fully become thoroughly decomposed; Simultaneously, adopt the clamp method fermentation to be convenient to monitor the bank temperature and change, be convenient to the turning collapse of setting, be convenient to keep the skin wet.The material fermentation is fermenting twice, and stevia rebaudiana dregs and pig manure are the material of processing through anaerobically fermenting, and then through aerobic stacking fermentation, has guaranteed the thoroughness of mixture attenuation degree.Contain abundant organic matter and glucide in the fermentation materials that fermentation obtains, can guarantee to add the surviving rate of PGPR bacterium, when producing granular bioorganic fertilizer, in granulation, oven dry, cooling, add the PGPR bacterium by the mode of dressing after the steps such as screening, can guarantee the activity of PGPR bacterium, reduce the rate of loss of microbial inoculum.
PGPR biological organic fertilizer provided by the invention be use through thoroughly, the stevia rebaudiana dregs and the pig manure mixture that fully become thoroughly decomposed make, and solved insufficient the causing of the mixture fermentation problem of " stinging seedling ".PGPR method for producing biological organic fertilizer provided by the invention is simple, with low cost, easy to utilize, solves to a certain extent the difficult problem that sweet Stevia processing enterprise waste material is stacked and processed, and can accomplish thoroughly utilization of waste material, energy-conserving and environment-protective.In addition, contain a large amount of organic matters, PGPR probiotics, npk nutrient and humic acids in the fertilizer by production method production of the present invention, can improve Soil structure, strengthen the resistance of crop, lower susceptible rate, promote plant growth, increase output, improve product quality.
Embodiment
Below by specific embodiment technical scheme of the present invention is elaborated.
Embodiment 1
The PGPR biological organic fertilizer that present embodiment provides makes by the production method that may further comprise the steps:
(1) preparation mixture
To mix through the pig manure of anaerobically fermenting with through the stevia rebaudiana dregs of anaerobically fermenting, and mix thoroughly, and make mixture, the weight percentage through the pig manure of anaerobically fermenting in the mixture is 35%; The C/N value of the mixture that makes is that 25, pH value is 6;
(2) mixture stacking fermentation
Mixture carries out the stacking fermentation, in mixture, add the starter colloid bacillus cereus during stacking fermentation, the addition of colloid bacillus cereus is 1 ‰ of mixture weight, the mixture clamp method is: lay one deck mixture, then on the mixture bed of material, spread one deck starter, sprinkle water on mixture during stacking, the weight percentage of water is controlled to be 55% in the mixture, the complete stockpile that obtains of mixture stacking; During 54 ℃ of stockpile temperature, stockpile is carried out turning, and keep the skin wet, make that the weight percentage of water remains 55% in the stockpile, the stockpile temperature remains 54-70 ℃ afterwards, ferments 10 days, and turning is 2 times between yeast phase, this moment, the stockpile homo(io)thermism was 65 ℃, free from extraneous odour shows and ferments fully that it is complete to ferment, make fermentation materials, the weight percentage of water is 20% in the fermentation materials;
(3) preparation powder
Fermentation materials is mixed with humic acids, inorganic nutrients, pulverize, make powder, the weight percentage of fermentation materials is 85% in the powder, and the weight percentage of humic acids is 13%, and the weight percentage of inorganic nutrients is 2%;
(4) preparation PGPR biological organic fertilizer
Preparation powdery PGPR biological organic fertilizer: add the PGPR bacterium in powder, the addition of PGPR bacterium is 2 ‰ of powder weight, stirs, and makes the PGPR biological organic fertilizer.
Embodiment 2
The PGPR biological organic fertilizer that present embodiment provides makes by the production method that may further comprise the steps:
(1) preparation mixture
To mix through the pig manure of anaerobically fermenting with through the stevia rebaudiana dregs of anaerobically fermenting, and mix thoroughly, and make mixture, the weight percentage through the pig manure of anaerobically fermenting in the mixture is 45%; The C/N value of the mixture that makes is that 23, pH value is 8;
(2) mixture stacking fermentation
Mixture carries out the stacking fermentation, in mixture, add the starter subtilis during stacking fermentation, the addition of subtilis is 5 ‰ of mixture weight, the mixture clamp method is: lay one deck mixture, then on the mixture bed of material, spread one deck starter, sprinkle water on mixture during stacking, the weight percentage of water is controlled to be 65% in the mixture, the complete stockpile that obtains of mixture stacking; During 58 ℃ of stockpile temperature, stockpile is carried out turning, and keep the skin wet, make that the weight percentage of water remains 65% in the stockpile, the stockpile temperature remains 54-70 ℃ afterwards, ferments 7 days, and turning is 3 times between yeast phase, this moment, the stockpile homo(io)thermism was 60 ℃, free from extraneous odour shows and ferments fully that it is complete to ferment, make fermentation materials, the weight percentage of water is 30% in the fermentation materials;
(3) preparation powder
Fermentation materials is mixed with humic acids, inorganic nutrients, pulverize, make powder, the weight percentage of fermentation materials is 80% in the powder, and the weight percentage of humic acids is 18%, and the weight percentage of inorganic nutrients is 2%;
(4) preparation PGPR biological organic fertilizer
Preparation particulate state PGPR biological organic fertilizer: with granulation for powders, oven dry, cooling, sub-sieve, make granulated fertilizer, by the mode of dressing that PGPR bacterium bag is surperficial to granulated fertilizer again, the addition of PGPR bacterium is 1 ‰ of powder weight, makes particulate state PGPR biological organic fertilizer.
Embodiment 3
The PGPR biological organic fertilizer that present embodiment provides makes by the production method that may further comprise the steps:
(1) preparation mixture
To mix through the pig manure of anaerobically fermenting with through the stevia rebaudiana dregs of anaerobically fermenting, and mix thoroughly, and make mixture, the weight percentage through the pig manure of anaerobically fermenting in the mixture is 45%; The C/N value of the mixture that makes is that 23, pH value is 7;
(2) mixture stacking fermentation
Mixture carries out the stacking fermentation, in mixture, add starter colloid bacillus cereus and subtilis during the stacking fermentation, the weight proportion of colloid bacillus cereus and subtilis is 1:1, the addition of starter is 3 ‰ of mixture weight, the mixture clamp method is: lay one deck mixture, then on the mixture bed of material, spread one deck starter, on mixture, sprinkle water during stacking, the weight percentage of water is controlled to be 60% in the mixture, the complete stockpile that obtains of mixture stacking; During 58 ℃ of stockpile temperature, stockpile is carried out turning, and keep the skin wet, make that the weight percentage of water remains 60% in the stockpile, the stockpile temperature remains 54-70 ℃ afterwards, ferments 7 days, and turning is 3 times between yeast phase, this moment, the stockpile homo(io)thermism was 60 ℃, free from extraneous odour shows and ferments fully that it is complete to ferment, make fermentation materials, the weight percentage of water is 25% in the fermentation materials;
(3) preparation powder
Fermentation materials is mixed with humic acids, inorganic nutrients, pulverize, make powder, the weight percentage of fermentation materials is 82% in the powder, and the weight percentage of humic acids is 17%, and the weight percentage of inorganic nutrients is 1%;
(4) preparation PGPR biological organic fertilizer
Preparation particulate state PGPR biological organic fertilizer: with granulation for powders, oven dry, cooling, sub-sieve, make granulated fertilizer, by the mode of dressing that PGPR bacterium bag is surperficial to granulated fertilizer again, the addition of PGPR bacterium is 2 ‰ of powder weight, makes particulate state PGPR biological organic fertilizer.
Inorganic nutrients among the embodiment 1-3 refers to nitrogen-phosphorus-potassium compound fertilizer.
The PGPR biological organic fertilizer that embodiment 1 provides is through field test, and phosphorus decomposing intensity reaches 30.76%, and soluble potassium concentration reaches 5.764 μ g/ml, improves nearly one times than existing bio-fertilizer.
The PGPR biological organic fertilizer that embodiment 1 provides is after big area is used, and crop yield can improve about 10-15%, and wherein peanut can improve output 15.6%, and wheat can improve output 11.5%, and corn can improve output 13.8%.
After using the PGPR biological organic fertilizer that embodiment 1 provides, the soil weight reduces by 13.6%, capillary porosity increases by 11.3%, illustrate that PGPR biological organic fertilizer that embodiment 1 provides can obviously improve the structure of soil, can obviously improve soil water-retaining, fertilizer conservation, ventilation capacity, promote the root system development, for farm crop provide comfortable growing environment.Simultaneously, rapid available phosphorus, available potassium, full nitrogen, full phosphorus and organic content exceed 51.2%, 79.6%, 41.3%, 69.7% and 77.1% than using other fertilizer respectively.The purpose of rapid stimulation plant growth be can reach after use in the field, seedling-slowing stage and farm crop starting stage reduced to the demand of nutrient.
Claims (8)
1. a PGPR biological organic fertilizer is characterized in that, makes by the production method that may further comprise the steps:
(1) preparation mixture
To mix through the pig manure of anaerobically fermenting with through the stevia rebaudiana dregs of anaerobically fermenting, and mix thoroughly, and make mixture, the weight percentage through the pig manure of anaerobically fermenting in the described mixture is 35-45%;
(2) mixture stacking fermentation
Described mixture carries out the stacking fermentation, in described mixture, add starter during the stacking fermentation, the addition of starter is the 1-5 ‰ of described mixture weight, the mixture clamp method is: lay one deck mixture, then on the mixture bed of material, spread the described starter of one deck, sprinkle water on mixture during stacking, the weight percentage of water is controlled to be 55-65% in the mixture, the complete stockpile that obtains of mixture stacking; During described stockpile temperature 〉=54 ℃, stockpile is carried out turning, and keep the skin wet, make that the weight percentage of water remains 55-65% in the stockpile, the stockpile temperature remains 54-70 ℃ afterwards, ferments 7-10 days, turning is 2-3 time between yeast phase, it is complete to ferment, and makes fermentation materials, and the weight percentage of water is 20-30% in the described fermentation materials;
(3) preparation powder
Described fermentation materials is mixed with humic acids, inorganic nutrients, pulverize, make powder, the weight percentage of fermentation materials is 80-85% in the described powder, and the weight percentage of described humic acids is 13-18%, and the weight percentage of inorganic nutrients is 1-2%;
(4) preparation PGPR biological organic fertilizer
Preparation powdery PGPR biological organic fertilizer: add the PGPR bacterium in described powder, the addition of described PGPR bacterium is the 1-2 ‰ of described powder weight, stirs, and makes the PGPR biological organic fertilizer;
Preparation particulate state PGPR biological organic fertilizer: with described granulation for powders, oven dry, cooling, sub-sieve, make granulated fertilizer, by the mode of dressing that PGPR bacterium bag is surperficial to described granulated fertilizer again, the addition of described PGPR bacterium is the 1-2 ‰ of described powder weight, makes particulate state PGPR biological organic fertilizer.
2. PGPR biological organic fertilizer according to claim 1 is characterized in that, the C/N value of the mixture that step (1) makes is 23-25, and the pH value is 6-8.
3. PGPR biological organic fertilizer according to claim 1 is characterized in that, described starter is aerobic thermophilic zymophyte.
4. PGPR biological organic fertilizer according to claim 3 is characterized in that, described aerobic thermophilic zymophyte is colloid bacillus cereus and/or subtilis.
5. PGPR biological organic fertilizer according to claim 1 is characterized in that, the addition of described starter is 3 ‰ of described mixture weight.
6. PGPR biological organic fertilizer according to claim 1 is characterized in that, the weight percentage of water is controlled to be 60% in the middle mixture of step (2).
7. PGPR biological organic fertilizer according to claim 1 is characterized in that, the weight percentage of water is 25% in the described fermentation materials.
8. the production method of the arbitrary described PGPR biological organic fertilizer of claim 1-7 is characterized in that, may further comprise the steps:
(1) preparation mixture
To mix through the pig manure of anaerobically fermenting with through the stevia rebaudiana dregs of anaerobically fermenting, and mix thoroughly, and make mixture, the weight percentage through the pig manure of anaerobically fermenting in the described mixture is 35-45%;
(2) mixture stacking fermentation
Described mixture carries out the stacking fermentation, in described mixture, add starter during the stacking fermentation, the addition of starter is the 1-5 ‰ of described mixture weight, the mixture clamp method is: lay one deck mixture, then on the mixture bed of material, spread the described starter of one deck, sprinkle water on mixture during stacking, the weight percentage of water is controlled to be 55-65% in the mixture, the complete stockpile that obtains of mixture stacking; When described stockpile temperature reaches more than 54 ℃, stockpile is carried out turning, and keep the skin wet, make that the weight percentage of water remains 55-65% in the stockpile, the stockpile temperature remains 54-70 ℃ afterwards, ferments 7-10 days, turning is 2-3 time between yeast phase, it is complete to ferment, and makes fermentation materials, and the weight percentage of water is 20-30% in the described fermentation materials;
(3) preparation powder
Described fermentation materials is mixed with humic acids, inorganic nutrients, pulverize, make powder, the weight percentage of fermentation materials is 80-85% in the described powder, and the weight percentage of described humic acids is 13-18%, and the weight percentage of inorganic nutrients is 1-2%;
(4) preparation PGPR biological organic fertilizer
Preparation powdery PGPR biological organic fertilizer: add the PGPR bacterium in described powder, the addition of described PGPR bacterium is the 1-2 ‰ of described powder weight, stirs, and makes the PGPR biological organic fertilizer;
Preparation particulate state PGPR biological organic fertilizer: with described granulation for powders, oven dry, cooling, sub-sieve, make granulated fertilizer, by the mode of dressing that PGPR bacterium bag is surperficial to described granulated fertilizer again, the addition of described PGPR bacterium is the 1-2 ‰ of described powder weight, makes particulate state PGPR biological organic fertilizer.
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| CN104876657A (en) * | 2015-05-15 | 2015-09-02 | 山东中创亿丰肥料有限公司 | Preparation method of efficient organic matter |
| CN104892109A (en) * | 2015-05-15 | 2015-09-09 | 山东中创亿丰肥料有限公司 | Biological organic fertilizer based on polypeptide trichelating |
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| CN104892109A (en) * | 2015-05-15 | 2015-09-09 | 山东中创亿丰肥料有限公司 | Biological organic fertilizer based on polypeptide trichelating |
| CN112830841A (en) * | 2021-01-22 | 2021-05-25 | 辽宁振兴生物科技发展有限公司 | Biological stimulator coated compound fertilizer, application and preparation method thereof |
| CN116217296A (en) * | 2023-03-17 | 2023-06-06 | 山东庞大生物集团有限公司 | Production process for carbon fixation and emission reduction of bio-organic fertilizer |
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