CN105218177A - Utilize silkworm excrement fermentation for the method for composite microbic bacterial fertilizer - Google Patents
Utilize silkworm excrement fermentation for the method for composite microbic bacterial fertilizer Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The present invention discloses a kind of silkworm excrement fermentation that utilizes for the method for composite microbic bacterial fertilizer, and its step is as follows: it is for subsequent use that the silkworm excrement matrix of 1, storing up tans by the sun sterilizing; 2, in subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, blown-ball Azotobacter and the streptomyces microflavus substratum after condition suitable separately and sterilization, 12 hours fermentation cultivations are carried out; 3, by 5 of above-mentioned fermentation culture kinds of strain inoculation in silkworm excrement substratum, add plastic film covering surface stack fermentation 10-15 hour; 4, after the silkworm excrement after fermentation is down to envrionment temperature naturally, spreads fertile heap out, after seasoning, namely obtain composite microbic bacterial fertilizer.Composite microbic bacterial fertilizer of the present invention is rich in the useful microorganism species of soil and corps nutrient material, can increase soil fertility, and improves soil structure and physicochemical character, increases the yield and quality of farm crop.
Description
Technical field
The present invention relates to a kind of production method of composite microbic bacterial fertilizer, to particularly relate to the remaining silkworm excrement stores of sericulture as matrix, take the method for the composite microbic bacterial fertilizer in multiple kinds of crops through fermentation.
Background technology
More than 1,800,000,000 mu, the existing arable land of China, uses chemical fertilizer about 1.5 hundred million tons every year, is chemical fertilizers production state maximum in the world, importer and use state.A large amount of production and use chemical fertilizer and not only consume the precious resources such as a large amount of coal, oil and natural gas, consumes a large amount of energy source and powers, also causes soil compaction and degenerate and environmental pollution widely.The part being applied in the chemical fertilizer in farmland inevitably enters river, river, lake, sea, cause the eutrophication of the water body in China inland and coastal waters, various algae and Herba Eichhorniae be explosive growth continually, and then rots, release toxin and biochemical oxygen demand, cause China's water body pollution in wide area.China's environmental pollution surveys report in recent years lists one of maximum source of pollution of China in chemical fertilizer.Therefore the alternative fertilizer of development environment friendly, has become a very urgent task.
Microbial-bacterial fertilizer is undoubtedly the best substitute of chemical fertilizer, and it is not only reproducible environmental friendly fertilizer, and can turn out the organic agronomic crop product of high-quality and high value.But microbial-bacterial fertilizer Vehicles Collected from Market sold and agriculturally use also has two distinct disadvantage, the first, bacterial classification and the effect of many products are single, extensively can not be adapted to various crop; The second, external commodity microbial-bacterial fertilizer often can not utilize local starting material with suiting measures to local conditions.These local raw material majorities are wastes of local agriculture production, not only reduce cost for the production of microbial-bacterial fertilizer, and eliminate local environmental pollution.
Over nearly 20 years, Guangxi has become that the maximum silkworm cultivation of China is large to economize, but the silkworm excrement that sericulture abandons (silkworm faeces, silkworm are sloughed off and the stores of remaining mulberry leaf) distributes stench, polluted source, becomes local main environmental pollutant.According to analysis, silkworm excrement contains organism 83.77 ~ 90.44%, ash content 9.56 ~ 16.23%, total nitrogen 1.91 ~ 3.60%.Total free aminoacids contained by silkworm excrement, once found out 13 seed amino acid such as leucine, Histidine, along with silkworm grows up, in excrement, leucine and histidine content are also cumulative many.Silkworm excrement is containing volume carotene.After macerating become thoroughly decomposed according to chemical examination 100 jin of dry silkworm excrement, nitrogenous 13.55%, phosphorus 8.37%, potassium ID.15%, calcium 1.16%, can improve soil, and one or two silkworm dung is executed down in field, can black one square chi of earth.Simultaneously also containing trace elements such as copper, iron, manganese, zinc, for plant-growth provides required nutritive ingredient.In addition, after silkworm faeces fermentation, also containing each amino acid, VITAMIN, protein, Plant hormones regulators,gibberellins, growth hormone, carbohydrate, nucleic acid, microbiotic etc., wherein have many physiologically active substances, they have regulating and controlling effect to crop growthing development, have preventive and therapeutic effect to crop pest.Ammonia in silkworm faeces, ammonium salt, microbiotic, can make some insects shy away, reduce and lay eggs on crop, suppresses the growth of worm's ovum, thus reduce insect pest.Thus silkworm excrement is the desirable feedstock of producing microbial-bacterial fertilizer, is the good thing of achieving many things at one stroke.
Retrieve the patent of following patent about silkworm excrement fertilizer:
Patent 1, application number: 201310632650.3; Applicant: Nong Keyuan bio tech ltd, Shandong; Contriver: Li Xikun; Denomination of invention: a kind of silkworm faeces bio-organic fertilizer and preparation method thereof; Summary: the present invention relates to bio-organic fertilizer manufacture craft manufacture field, particularly relate to a kind of silkworm faeces bio-organic fertilizer and preparation method thereof, its technical scheme is: silkworm faeces fermented product 60-80 part, leaf fermented product 10-20 part, rice bran fermented product 30-50 part, animal excrement fermented product 1-3 part, washing water of rice 15-20 part, feature of the present invention makes simply, cost is low, fertilizer efficiency is high, and the interpolation of silkworm faeces, leaf, rice bran fermented product can be nursed one's health soil, activate Soil Microorganism enlivens rate, overcomes soil compaction, increases soil air permeability.Reduce water loss and evaporation, alleviate arid pressure, fertilizer conservation, minimizing chemical fertilizer, alleviate saline and alkaline infringement, when reduce fertilizer amount or progressively replacing fertilizer, animal dung after fermentable process and plant fermentation fertilizer have the advantage of fertilizer, microbial-bacterial fertilizer and humic acid fertilizer concurrently, the demand of crop to nutrient can be met largely, reach the object of high-quality, high yield.
Patent, 2 application numbers: 201410221427.4; Applicant: this Fei Ye company limited of Guangxi Di Yuan; Contriver: Wang Xiuhai, Chen Zuojun; Denomination of invention: a kind of organic fertilizer additive containing silkworm excrement and preparation method thereof; Summary: the present invention discloses a kind of organic fertilizer additive containing silkworm excrement and preparation method thereof, relate to fertilizer additive technical field, its composition includes the raw material of following weight percents: 10 parts, wheat bran, silkworm excrement 50 parts, Semen Maydis powder 20 parts, microbial bacteria 0.1 part, making method is wheat bran, Semen Maydis powder and silkworm excrement are mixed with microbial bacteria, stir laggard row compost fermentation, regulates water ratio to be 60%; When temperature rises to 70 degree, turning ventilation is carried out to fertilizer heap, stirred once every 24 hours, within 18-20 days, complete fermentation, obtain this additive; Organic fertilizer additive of the present invention, can not only provide full nutrition for farm crop, increases fertilizer efficiency, and can increase and upgrade the soil organism, promotes microbial reproduction.
Patent 3, application number: 201410232538.5; Applicant: Qian Feng; Contriver: Qian Feng, Fang Xixiu, Chen Ming, Qian Wei; Denomination of invention: containing the Biofertiliser composition of pig manure, silkworm excrement, rice husk; Summary: the present invention relates to the bio-feritlizer containing salt, fermented bacterium and organic matter, efficiently solve the hardening soil sclerosis caused because of long-term application chemical fertilizer, environmental pollution and the chemical residual to grain, vegetables cause the problems such as the harm of human body.It is characterized in that the component by scientific process primarily of 8 kinds of above-mentioned weight parts such as dry pig manure 45-55 part, silkworm excrement 5-10 part, fermented bacterium 0.1 part, straw powder 5-10 part, rice husk 5-10 part, dregs of beans 10-20 part, mud 10-20 part, salt 3-5 parts forms, the present invention fills a prescription accurately, making method is simple, biological recovery utilization ratio is high, pollution-free, to human body without harm.
Patent 4, application number: 201410450360.1; Applicant: Guangxi San Bao agricultural science and technology company limited; Contriver: Su Lin, Huang Dongling, Zou Chaoxian, Liao Huan, Guo Huaqi, Yu Musheng, circumfluence river; Denomination of invention: a kind of method of quick fermentation silkworm excrement organic fertilizer; Summary: a kind of method of quick fermentation silkworm excrement organic fertilizer, in 4 hours after silk cocoon is put on an arrow, throws in 10-20 kilogram of composite bacteria by every 1000 kilograms of silkworm excrements, evenly be sprinkling upon the surface of silkworm excrement, then in 4 hours, the silkworm excrement sprinkled with composite bacteria is stacked by wall limit or level land, during stacking, face will form ellipse or circle, stack and be highly more than 100 centimetres, can not compacting during stacking, can not epiphragma, keep silkworm excrement to loosen, ventilated, to prevent water from entering during fermentation, rain and sun; Stack fermentation time and reach 10-20 hour, silkworm excrement meeting automatic heating is to 60-70 DEG C, and carry out turning silkworm excrement and again ferment once, fermentation time is 10-14 hour whole fermenting-ripening again, obtains silkworm excrement organic fertilizer; Operation is simple for present method, and fermentation time is short, and production efficiency is high, and silkworm excrement organic fertilizer is particulate state, and the quality of fertilizer is high, and effect is good, has good economic benefit, social benefit and ecological benefits.
Patent 5, application number: 201410477653.9, applicant: this Fei Ye company limited of Guangxi Di Yuan, contriver: Chen Zuojun, Wang Xiuhai, denomination of invention: a kind of silkworm excrement organic fertilizer and preparation method, summary: the present invention discloses a kind of silkworm excrement organic fertilizer and preparation method, relate to bio-organic fertilizer technical field, it is by bacterium chaff 25-35 part, become thoroughly decomposed fermentation silkworm excrement 25-40 part, chicken manure 10-15 part that pyroprocessing is crossed, phosphate fertilizer 5-10 part, potash fertilizer 5-10 part is made, the silkworm excrement of fermentation of becoming thoroughly decomposed mixes with bacterium chaff by preparation method, wet heap carries out spontaneous fermentation, ferment after 5 days, add phosphate fertilizer and potash fertilizer mixes, granulation, dry, obtain, this organic fertilizer organic content is high, the chicken manure that silkworm excrement and pyroprocessing are crossed is combined, its nutritive element contained is comprehensive, fertilizer efficiency is long, the growth of fruits and vegetables can be accelerated, shorten planting time, reduce cultivation cost.
Patent 6, application number: 201410480357.4, applicant: this Fei Ye company limited of Guangxi Di Yuan, contriver: Chen Zuojun, Wang Xiuhai, denomination of invention: a kind of silkworm excrement organic fertilizer and preparation method thereof, summary: the present invention discloses a kind of silkworm excrement organic fertilizer and preparation method thereof, relate to bio-organic fertilizer technical field, it is by bacterium chaff 25-35 part, become thoroughly decomposed fermentation silkworm excrement 25-40 part, fish scale 10-15 part that pyroprocessing is crossed, phosphate fertilizer 5-10 part, potash fertilizer 5-10 part is made, the silkworm excrement of fermentation of becoming thoroughly decomposed mixes with bacterium chaff by preparation method, wet heap carries out spontaneous fermentation, ferment after 5 days, add phosphate fertilizer and potash fertilizer mixes, granulation, dry, obtain, this organic fertilizer organic content is high, the fish scale that silkworm excrement and pyroprocessing are crossed is combined, its nutritive element contained is comprehensive, fertilizer efficiency is long, the growth of fruits and vegetables can be accelerated, shorten planting time, reduce cultivation cost.
Patent 7, application number: 201410482271.5, applicant: this Fei Ye company limited of Guangxi Di Yuan, contriver: Chen Zuojun, Wang Xiuhai, denomination of invention: a kind of silkworm excrement organic fertilizer and preparation method thereof, summary: a kind of silkworm excrement organic fertilizer and preparation method thereof, relate to bio-organic fertilizer technical field, it is by bacterium chaff 30-45 part, become thoroughly decomposed fermentation silkworm excrement 30-45 part, phosphate fertilizer 5-10 part, potash fertilizer 5-10 part is made, the silkworm excrement of fermentation of becoming thoroughly decomposed mixes with bacterium chaff by preparation method, wet heap carries out spontaneous fermentation, ferment after 5 days, add phosphate fertilizer and potash fertilizer mixes, granulation, dry, obtain, this organic fertilizer organic content is high, its nutritive element contained is comprehensive, fertilizer efficiency is long, the growth of fruits and vegetables can be accelerated, shorten planting time, reduce cultivation cost.
Technology and the technology of the present invention of its Patent 2 and patent 4 are the closest, 3 inventions are all mentioned to microbial bacteria, patent 2 discloses a kind of organic fertilizer additive containing silkworm excrement and preparation method thereof, relate to fertilizer additive technical field, its composition includes the raw material of following weight percents: 10 parts, wheat bran, silkworm excrement 50 parts, Semen Maydis powder 20 parts, microbial bacteria 0.1 part, making method is wheat bran, Semen Maydis powder and silkworm excrement are mixed with microbial bacteria, one time fermentation, and its unexposed microbial bacteria specifically which kind of bacterial classification.Patent 4 discloses a kind of method of quick fermentation silkworm excrement organic fertilizer, and in 4 hours after silk cocoon is put on an arrow, throw in 10-20 kilogram of composite bacteria by every 1000 kilograms of silkworm excrements, composite bacteria is wherein microbiobacterial agent: colloid bacillus cereus 0.02-0.04 part; Bacillus licheniformis 0.01-0.03 part; Streptomyces jingyangensis whey powder 0.01-0.03 part; Azotobacter vinelandii 0.01-0.02 part; Aspergillus spore powder 0.04-0.08 part; Thermophilic coccus 0.01-0.03 part; Enterococcus faecalis 0.01-0.02 part; Antibacterial peptide thalline 0.02-0.04 part.Wherein strain inoculation time limitation " silk cocoon put on an arrow after 4 hours in ", fermentation process is that the silkworm excrement that will be mixed with composite bacteria is stacked by wall limit or level land.But disclosed by the invention is that a kind of silkworm excrement that utilizes prepares the method for composite microbic bacterial fertilizer through two-step fermentation, and its step is as follows: 1, silkworm excrement matrix is for subsequent use; 2, in subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, blown-ball Azotobacter and the streptomyces microflavus substratum after condition suitable separately and sterilization, the first step fermentation culture of 12 hours is carried out; 3, by 5 of above-mentioned fermentation culture kinds of strain inoculation in silkworm excrement substratum, add plastic film covering surface stack, carry out second step fermentation culture 10-15 hour; 4, after the silkworm excrement after fermentation is down to envrionment temperature naturally, spreads fertile heap out, after seasoning, namely obtain composite microbic bacterial fertilizer.The first step fermentation scale-up of the present invention quantity of bacterial classification, second step fermentation makes 5 kinds of bacterial classifications breed in silkworm excrement and form the bacterium colony of symbiosis, thus composite microbic bacterial fertilizer is rich in the useful microorganism species of soil and corps nutrient material, can increase soil fertility, improve soil structure and physicochemical character, increase the yield and quality of farm crop.
Summary of the invention
The present invention utilizes silkworm excrement fermentation for the method for composite microbic bacterial fertilizer, the composite microbic bacterial fertilizer of the corps nutrient material containing multiple-microorganism, in order to increase soil fertility, improves soil structure and physical and chemical quality, promotes the seed output and quality of farm crop.
Technical scheme of the present invention is:
Utilize silkworm excrement fermentation for the method for composite microbic bacterial fertilizer, step is as follows:
(1) silkworm excrement stored up is spread out, under burning sun, tan by the sun sterilization in 3 days, or the 1-3 hour that sterilizes under ultraviolet sterilizing lamp, be then dried to moisture content weight content below 10%, be crushed to below 50 orders, obtain silkworm excrement matrix for subsequent use; Described silkworm excrement refers to remaining silkworm faeces of breeding silkworms, silkworm sloughs off and the stores of remaining mulberry leaf;
(2) bacterium culture medium is placed in stainless steel drum, rotate 28 ~ 35 minutes at the temperature of the rotating speeds of 120 revs/min and 110 ~ 130 DEG C, be down to after room temperature extremely moistening with cleaning water spray substratum until substratum temperature, 5 kinds of different bacterial classifications are inoculated respectively by the part by weight of bacterial classification and substratum 1:100, at the rotating speed of 120 revs/min, under the environment of pH=7.4 and temperature 40 ~ 50 DEG C, difference fermentation culture 12 hours, obtains the bacterial classification of fermentation culture; Described 5 kinds different bacterial classifications are subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, blown-ball Azotobacter and streptomyces microflavus;
(3) in 100 parts of silkworm excrement matrix, add 5 parts of molasses, 10 parts of starch and 5 parts of dregs of beans, sprinkle cleaning water is to moistening, after mixing and stirring by the bacterial classification of above-mentioned fermentation culture according to part by weight: subtilis: bacillusmusilaginosiengineering: bacillus amyloliquefaciens: blown-ball Azotobacter: streptomyces microflavus=8:1:1:1:1, be inoculated in silkworm excrement substratum, mixing and stirring, substratum after inoculation sprays a small amount of cleaning water, to moistening, add plastic film covering surface and stack fermentation 10-15 hour; The gross weight of described 5 kinds of bacterial classifications and the weight ratio of silkworm excrement matrix are 0.5-1:100;
(4), after the silkworm excrement after fermentation is down to envrionment temperature naturally, spreads fertile heap out, after seasoning, namely obtain composite microbic bacterial fertilizer;
In described (2) step, the parts by weight of raw materials array of bacterium culture medium becomes: starch 5-15 part, molasses 3-8 part, dregs of beans 3-8 part, phosphate fertilizer 0.01-0.05 part, Paris white 0.1-0.5 part, magnesium sulfate 0.01-0.05 part.
Above-described starch is potato starch or cereal starch.Described dregs of beans is soybean meal, peanut press pulp, cottonseed meal or leached tea oil slag.Described phosphate fertilizer is dipotassium hydrogen phosphate, calcium superphosphate or ammonium phosphate.
In described (4) step, after fermentation culture 10-15 hour, live strain quantity is greater than 200,000,000/gram, pH value is between 5.0 ~ 7.0.
Compared with prior art, positively effect of the present invention is:
1, the present invention utilizes silkworm excrement fermentation for the method for composite microbic bacterial fertilizer, for matrix with the remaining silkworm excrement stores of sericulture, adopt two-step fermentation, prepare the composite microbic bacterial fertilizer contained the useful microorganism species of soil and corps nutrient material, in order to increase soil fertility, improve soil structure and physical and chemical quality, promote the seed output and quality of farm crop.
2, composite microbic bacterial fertilizer of the present invention is through two-step fermentation (the independence fermentation of bacterial classification and the mixed fermentation in silkworm excrement matrix), improves the quantity of probiotics in compound bacterial fertilizer, takes full advantage of the nutritive ingredient in silkworm excrement simultaneously, reduce the production cost of bacterial manure.Second step mixed fermentation makes 5 kinds of bacterial classifications form activated symbiosis flora, plays good action to the structure and physicochemical character improving soil.There is not any residue, raffinate aborning in present method, without any environmental pollution.
3, the present invention utilizes silkworm excrement fermentation for the method for composite microbic bacterial fertilizer, and adopt the remaining silkworm excrement stores of sericulture to be matrix, can improve soil, one or two silkworm dung is executed down in field, can black one square chi of earth.Simultaneously also containing trace elements such as copper, iron, manganese, zinc, for plant-growth provides required nutritive ingredient.In addition, after silkworm faeces fermentation, also containing each amino acid, VITAMIN, protein, Plant hormones regulators,gibberellins, growth hormone, carbohydrate, nucleic acid, microbiotic etc., wherein have many physiologically active substances, they have regulating and controlling effect to crop growthing development, have preventive and therapeutic effect to crop pest.Ammonia in silkworm faeces, ammonium salt, microbiotic, can make some insects shy away, reduce and lay eggs on crop, suppresses the growth of worm's ovum, thus reduce insect pest.
Embodiment
Below in conjunction with embodiments of the invention, be clearly and completely described the technical scheme in invention, described embodiment is only a part of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, other embodiment that those of ordinary skill in the art obtain under the prerequisite not making creative work, all belongs to the scope of protection of the invention.
Embodiment 1
Utilize silkworm excrement fermentation for the method for composite microbic bacterial fertilizer, step is as follows:
(1) silkworm excrement stored up is spread out, under burning sun, tan by the sun sterilization in 3 days, be then dried to moisture content weight content 10%, be crushed to 50 orders, obtain silkworm excrement matrix for subsequent use; Described silkworm excrement refers to remaining silkworm faeces of breeding silkworms, silkworm sloughs off and the stores of remaining mulberry leaf;
(2) bacterium culture medium is placed in stainless steel drum, rotate 28 minutes at the temperature of the rotating speeds of 120 revs/min and 110 DEG C, be down to after room temperature extremely moistening with cleaning water spray substratum until substratum temperature, 5 kinds of different bacterial classifications are inoculated respectively by the part by weight of bacterial classification and substratum 1:100, at the rotating speed of 120 revs/min, under the environment of pH=7.4 and temperature 40 DEG C, difference fermentation culture 12 hours, obtains the bacterial classification of fermentation culture; Described 5 kinds different bacterial classifications are subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, blown-ball Azotobacter and streptomyces microflavus;
(3) in 100 parts of silkworm excrement matrix, add 5 parts of molasses, 10 portions of tapioca (flour)s and 5 parts of soybean meal, sprinkle cleaning water is to moistening, after mixing and stirring by the bacterial classification of above-mentioned fermentation culture according to part by weight: subtilis: bacillusmusilaginosiengineering: bacillus amyloliquefaciens: blown-ball Azotobacter: streptomyces microflavus=8:1:1:1:1, be inoculated in silkworm excrement substratum, mixing and stirring, substratum after inoculation sprays a small amount of cleaning water, to moistening, add plastic film covering surface and stack fermentation 10 hours; The gross weight of described 5 kinds of bacterial classifications and the weight ratio of silkworm excrement matrix are 0.5:100;
(4), after the silkworm excrement after fermentation is down to envrionment temperature naturally, spreads fertile heap out, after seasoning, namely obtain composite microbic bacterial fertilizer.
In described (2) step, the parts by weight of raw materials array of bacterium culture medium becomes: tapioca (flour) 5 parts, 3 parts, molasses, soybean meal 3 parts, dipotassium hydrogen phosphate 0.01 part, Paris white 0.1 part, 0.01 part, magnesium sulfate.
In described (4) step, fermentation culture is after 10 hours, and live strain quantity reaches 2.8 hundred million/gram, pH value, 6.8, meets Ny227-1994 specified standards.
Embodiment 2
Utilize silkworm excrement fermentation for the method for composite microbic bacterial fertilizer, step is as follows:
(1) spread out by the silkworm excrement stored up, sterilize 1-3 hour under ultraviolet sterilizing lamp, is then dried to moisture content weight content 8%, is crushed to 40 orders, obtain silkworm excrement matrix for subsequent use; Described silkworm excrement refers to remaining silkworm faeces of breeding silkworms, silkworm sloughs off and the stores of remaining mulberry leaf;
(2) bacterium culture medium is placed in stainless steel drum, rotate 32 minutes at the temperature of the rotating speeds of 120 revs/min and 120 DEG C, be down to after room temperature extremely moistening with cleaning water spray substratum until substratum temperature, 5 kinds of different bacterial classifications are inoculated respectively by the part by weight of bacterial classification and substratum 1:100, at the rotating speed of 120 revs/min, under the environment of pH=7.4 and temperature 45 C, difference fermentation culture 12 hours, obtains the bacterial classification of fermentation culture;
(3) in 100 parts of silkworm excrement matrix, add 5 parts of molasses, 10 parts of yam starchs and 5 parts of peanut press pulps, sprinkle cleaning water is to moistening, after mixing and stirring by the bacterial classification of above-mentioned fermentation culture according to part by weight: subtilis: bacillusmusilaginosiengineering: bacillus amyloliquefaciens: blown-ball Azotobacter: streptomyces microflavus=8:1:1:1:1, be inoculated in silkworm excrement substratum, mixing and stirring, substratum after inoculation sprays a small amount of cleaning water, to moistening, add plastic film covering surface and stack fermentation 12 hours; The gross weight of described 5 kinds of bacterial classifications and the weight ratio of silkworm excrement matrix are 0.8:100;
(4), after the silkworm excrement after fermentation is down to envrionment temperature naturally, spreads fertile heap out, after seasoning, namely obtain composite microbic bacterial fertilizer.
In described (2) step, the parts by weight of raw materials array of bacterium culture medium becomes: yam starch 10 parts, 5 parts, molasses, peanut press pulp 5 parts, calcium superphosphate 0.03 part, Paris white 0.3 part, 0.03 part, magnesium sulfate.
In described (2) step, described 5 kinds different bacterial classifications are subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, blown-ball Azotobacter and streptomyces microflavus.
In described (4) step, fermentation culture is after 12 hours, and live strain quantity reaches 3.0 hundred million/gram, pH value, 5.0, meets Ny227-1994 specified standards.
Embodiment 3
Utilize silkworm excrement fermentation for the method for composite microbic bacterial fertilizer, step is as follows:
(1) silkworm excrement stored up is spread out, under burning sun, tan by the sun sterilization in 3 days, or at ultraviolet disinfection sterilization 1-3 hour, be then dried to moisture content weight content below 10%, be crushed to below 50 orders, obtain silkworm excrement matrix for subsequent use; Described silkworm excrement refers to remaining silkworm faeces of breeding silkworms, silkworm sloughs off and the stores of remaining mulberry leaf;
(2) bacterium culture medium is placed in stainless steel drum, rotate 35 minutes at the temperature of the rotating speeds of 120 revs/min and 130 DEG C, be down to after room temperature extremely moistening with cleaning water spray substratum until substratum temperature, 5 kinds of different bacterial classifications are inoculated respectively by the part by weight of bacterial classification and substratum 1:100, at the rotating speed of 120 revs/min, under the environment of pH=7.4 and temperature 50 C, difference fermentation culture 12 hours, obtains the bacterial classification of fermentation culture; Described 5 kinds different bacterial classifications are subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, blown-ball Azotobacter and streptomyces microflavus;
(3) in 100 parts of silkworm excrement matrix, add 5 parts of molasses, 10 parts of W-Gums and 5 parts of cottonseed meal, sprinkle cleaning water is to moistening, after mixing and stirring by the bacterial classification of above-mentioned fermentation culture according to part by weight: subtilis: bacillusmusilaginosiengineering: bacillus amyloliquefaciens: blown-ball Azotobacter: streptomyces microflavus=8:1:1:1:1, be inoculated in silkworm excrement substratum, mixing and stirring, the gross weight base of cultivation after inoculation sprays a small amount of cleaning water, to moistening, add plastic film covering surface and stack fermentation 15 hours; The weight ratio of described 5 kinds of bacterial classifications and silkworm excrement matrix is 1:100;
(4), after the silkworm excrement after fermentation is down to envrionment temperature naturally, spreads fertile heap out, after seasoning, namely obtain composite microbic bacterial fertilizer.
In described (2) step, the parts by weight of raw materials array of bacterium culture medium becomes: W-Gum 15 parts, 8 parts, molasses, cottonseed meal 8 parts, 0.05 part, ammonium phosphate, Paris white 0.5 part, 0.05 part, magnesium sulfate.
In described (4) step, fermentation culture is after 15 hours, and live strain quantity reaches 3.1 hundred million/gram, meet Ny227-1994 specified standards.
Embodiment 4
The composite microbic bacterial fertilizer of preparation in embodiment 1 is used for peanut cultivation, and contrasts with 38% potassium sulfate compound fertilizer that Stanley Fertilizer Co., Ltd produces.The red soil soil plantation peanut varieties sweet osmanthus 22 of the medium fertility in Guangxi, under identical cultivation, nursing condition, the plot of using microbial compound bacterial fertilizer obtains the output higher than the plot of 38% potassium sulfate compound fertilizer using Stanley.Use the Microflora in the soil of composite microbic bacterial fertilizer and organic content all higher than comparative example.Concrete data are shown in table 1.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is by claims, instead of above-mentioned explanation limits, all changes be therefore intended in the implication of the equivalency by falling within claim and scope are included in the present invention.
In addition, should emphasize, although this specification sheets is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification sheets is only for clarity sake, those skilled in the art should by specification sheets integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (4)
1. utilize silkworm excrement fermentation for the method for composite microbic bacterial fertilizer, it is characterized in that, step is as follows:
(1) silkworm excrement stored up is spread out, under burning sun, tan by the sun sterilization in 3 days, or the 1-3 hour that sterilizes under ultraviolet sterilizing lamp, be then dried to moisture content weight content below 10%, be crushed to below 50 orders, obtain silkworm excrement matrix for subsequent use;
(2) bacterium culture medium is placed in stainless steel drum, rotate 28 ~ 35 minutes at the temperature of the rotating speeds of 120 revs/min and 110 ~ 130 DEG C, be down to after room temperature extremely moistening with cleaning water spray substratum until substratum temperature, 5 kinds of different bacterial classifications are inoculated respectively by the part by weight of bacterial classification and substratum 1:100, at the rotating speed of 120 revs/min, under the environment of pH=7.4 and temperature 40 ~ 50 DEG C, difference fermentation culture 12 hours, obtains 5 kinds of bacterial classifications of fermentation culture; Described 5 kinds different bacterial classifications are subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, blown-ball Azotobacter and streptomyces microflavus;
(3) in 100 parts of silkworm excrement matrix, add 5 parts of molasses, 10 parts of starch and 5 parts of dregs of beans, sprinkle cleaning water is to moistening, after mixing and stirring by 5 of above-mentioned fermentation culture kinds of bacterial classifications according to part by weight: subtilis: bacillusmusilaginosiengineering: bacillus amyloliquefaciens: blown-ball Azotobacter: streptomyces microflavus=8:1:1:1:1, be inoculated in silkworm excrement substratum, mixing and stirring, substratum after inoculation sprays a small amount of cleaning water, to moistening, add plastic film covering surface and stack fermentation 10-15 hour; The gross weight of described 5 kinds of bacterial classifications and the weight ratio of silkworm excrement matrix are 0.5-1:100;
(4), after the silkworm excrement after fermentation is down to envrionment temperature naturally, spreads fertile heap out, after seasoning, namely obtain composite microbic bacterial fertilizer;
Described silkworm excrement refers to remaining silkworm faeces of breeding silkworms, silkworm sloughs off and the stores of remaining mulberry leaf.
2. the silkworm excrement fermentation that utilizes according to claim 1 is for the method for composite microbic bacterial fertilizer, it is characterized in that: in described (2) step, the parts by weight of raw materials array of bacterium culture medium becomes: starch 5-15 part, molasses 3-8 part, dregs of beans 3-8 part, phosphate fertilizer 0.01-0.05 part, Paris white 0.1-0.5 part, magnesium sulfate 0.01-0.05 part.
3. the silkworm excrement fermentation that utilizes according to claim 1 and 2 is for the method for composite microbic bacterial fertilizer, it is characterized in that: described starch is potato starch or cereal starch; Described dregs of beans is soybean meal, peanut press pulp, cottonseed meal or leached tea oil slag; Described phosphate fertilizer is dipotassium hydrogen phosphate, calcium superphosphate or ammonium phosphate.
4. the silkworm excrement fermentation that utilizes according to claim 1 is for the method for composite microbic bacterial fertilizer, it is characterized in that: in described (4) step, after fermentation culture 10-15 hour, and live strain quantity is greater than 200,000,000/gram, pH value is between 5.0 ~ 7.0.
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