CN101756042A - Application of cupric glutamate or derivatives thereof as feed additive for promoting animal growth - Google Patents
Application of cupric glutamate or derivatives thereof as feed additive for promoting animal growth Download PDFInfo
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- CN101756042A CN101756042A CN 201010122625 CN201010122625A CN101756042A CN 101756042 A CN101756042 A CN 101756042A CN 201010122625 CN201010122625 CN 201010122625 CN 201010122625 A CN201010122625 A CN 201010122625A CN 101756042 A CN101756042 A CN 101756042A
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- China
- Prior art keywords
- copper
- cupric glutamate
- cupric
- glutamate
- derivatives
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Links
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 title claims abstract description 138
- 229930195712 glutamate Natural products 0.000 title claims abstract description 118
- 230000012010 growth Effects 0.000 title claims abstract description 48
- 241001465754 Metazoa Species 0.000 title claims abstract description 32
- 230000001737 promoting effect Effects 0.000 title claims abstract description 21
- 239000003674 animal food additive Substances 0.000 title abstract description 4
- 229910001431 copper ion Inorganic materials 0.000 claims abstract description 55
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000010949 copper Substances 0.000 claims abstract description 48
- 229910052802 copper Inorganic materials 0.000 claims abstract description 45
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 24
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 22
- 235000013922 glutamic acid Nutrition 0.000 claims description 22
- 239000004220 glutamic acid Substances 0.000 claims description 22
- 239000013522 chelant Substances 0.000 claims description 17
- 150000001875 compounds Chemical class 0.000 claims description 16
- 235000019730 animal feed additive Nutrition 0.000 claims description 13
- 239000007952 growth promoter Substances 0.000 claims description 12
- 150000003839 salts Chemical class 0.000 claims description 10
- 235000001014 amino acid Nutrition 0.000 claims description 9
- 150000001413 amino acids Chemical class 0.000 claims description 9
- 230000015572 biosynthetic process Effects 0.000 claims description 9
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 6
- LCQLHJZYVOQKHU-VKHMYHEASA-N carglumic acid Chemical compound NC(=O)N[C@H](C(O)=O)CCC(O)=O LCQLHJZYVOQKHU-VKHMYHEASA-N 0.000 claims description 5
- -1 amide compound Chemical class 0.000 claims description 4
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 3
- 239000003513 alkali Substances 0.000 claims description 3
- BBJIPMIXTXKYLZ-UHFFFAOYSA-N isoglutamic acid Chemical compound OC(=O)CC(N)CC(O)=O BBJIPMIXTXKYLZ-UHFFFAOYSA-N 0.000 claims description 3
- 108010016626 Dipeptides Proteins 0.000 claims description 2
- 150000001408 amides Chemical class 0.000 claims description 2
- 230000003698 anagen phase Effects 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 abstract description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 38
- 230000000694 effects Effects 0.000 abstract description 16
- 230000002829 reductive effect Effects 0.000 abstract description 4
- 231100000331 toxic Toxicity 0.000 abstract description 4
- 230000002588 toxic effect Effects 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract description 2
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000003115 biocidal effect Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- RMNCNUNUQBLASX-UHFFFAOYSA-N 2-aminoacetic acid;copper Chemical compound [Cu].NCC(O)=O RMNCNUNUQBLASX-UHFFFAOYSA-N 0.000 abstract 1
- 239000005752 Copper oxychloride Substances 0.000 abstract 1
- 239000000654 additive Substances 0.000 abstract 1
- 230000000996 additive effect Effects 0.000 abstract 1
- HKMOPYJWSFRURD-UHFFFAOYSA-N chloro hypochlorite;copper Chemical compound [Cu].ClOCl HKMOPYJWSFRURD-UHFFFAOYSA-N 0.000 abstract 1
- 229910000365 copper sulfate Inorganic materials 0.000 abstract 1
- 229940049906 glutamate Drugs 0.000 description 101
- 238000012360 testing method Methods 0.000 description 42
- 239000000243 solution Substances 0.000 description 35
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 25
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 description 24
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 23
- 241000282898 Sus scrofa Species 0.000 description 23
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 23
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 21
- 238000000034 method Methods 0.000 description 17
- 238000002360 preparation method Methods 0.000 description 11
- 231100000111 LD50 Toxicity 0.000 description 10
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- 241000282887 Suidae Species 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- FPVGTPBMTFTMRT-NSKUCRDLSA-L fast yellow Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 FPVGTPBMTFTMRT-NSKUCRDLSA-L 0.000 description 6
- 239000000463 material Substances 0.000 description 6
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- SEWIYICDCVPBEW-BYPYZUCNSA-N L-glutamate methyl ester Chemical compound COC(=O)[C@@H](N)CCC(O)=O SEWIYICDCVPBEW-BYPYZUCNSA-N 0.000 description 4
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- XBJZQKABKZDDGY-DFWYDOINSA-N (2s)-2-aminopentanedioic acid;copper Chemical compound [Cu].OC(=O)[C@@H](N)CCC(O)=O XBJZQKABKZDDGY-DFWYDOINSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 208000005374 Poisoning Diseases 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- LYPSLPXPGMCAHP-DFWYDOINSA-N [Cu].C(N)(=O)N[C@@H](CCC(=O)O)C(=O)O Chemical compound [Cu].C(N)(=O)N[C@@H](CCC(=O)O)C(=O)O LYPSLPXPGMCAHP-DFWYDOINSA-N 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N lysine Chemical compound NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 3
- 125000001288 lysyl group Chemical group 0.000 description 3
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
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- 241000196324 Embryophyta Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- UGTZHPSKYRIGRJ-YUMQZZPRSA-N Lys-Glu Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(O)=O)CCC(O)=O UGTZHPSKYRIGRJ-YUMQZZPRSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
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- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical class [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
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- 108010009298 lysylglutamic acid Proteins 0.000 description 2
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- 239000004223 monosodium glutamate Substances 0.000 description 2
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- 239000000126 substance Substances 0.000 description 2
- LITQZINTSYBKIU-UHFFFAOYSA-F tetracopper;hexahydroxide;sulfate Chemical compound [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[Cu+2].[Cu+2].[Cu+2].[Cu+2].[O-]S([O-])(=O)=O LITQZINTSYBKIU-UHFFFAOYSA-F 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- 241000459479 Capsula Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 241000370738 Chlorion Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 206010021135 Hypovitaminosis Diseases 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 241001508687 Mustela erminea Species 0.000 description 1
- 206010029164 Nephrotic syndrome Diseases 0.000 description 1
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- 101710117971 Peptide Y Proteins 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 241000282485 Vulpes vulpes Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 206010048259 Zinc deficiency Diseases 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
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- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- BESJRHHIPGWPTC-UHFFFAOYSA-N azane;copper Chemical compound N.[Cu] BESJRHHIPGWPTC-UHFFFAOYSA-N 0.000 description 1
- 235000014590 basal diet Nutrition 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
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- VVYPIVJZLVJPGU-UHFFFAOYSA-L copper;2-aminoacetate Chemical compound [Cu+2].NCC([O-])=O.NCC([O-])=O VVYPIVJZLVJPGU-UHFFFAOYSA-L 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 229940011405 cupric glycinate Drugs 0.000 description 1
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- 238000001514 detection method Methods 0.000 description 1
- ALKZAGKDWUSJED-UHFFFAOYSA-N dinuclear copper ion Chemical compound [Cu].[Cu] ALKZAGKDWUSJED-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
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- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
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Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses application of cupric glutamate or derivatives thereof as feed additive for promoting animal growth; the cupric glutamate which has the advantages of low water solubility, strong acid resistance and high bioavailability is firstly found, the cupric glutamate is used as the feed additive for promoting animal growth and has high safety and antibiosis activity compared with other copper sources, such as copper sulfate, copper oxychloride, glycine copper and the like, the additive amount of the copper ions can reduce 60-80 percent, the production cost is reduced and the environment pollution is reduced, and the toxic and side effect of high copper to the animals is reduced simultaneously.
Description
Technical field
The present invention be more particularly directed to of the application of a kind of cupric glutamate or derivatives thereof, belong to field of fodder as animal feed additive for promoting growth.
Background technology
Copper is one of essential trace element of animal, participates in hematopoiesis, metabolism, growth and breeding and the activity such as disease-resistant of animal.Animal to the nutritional requirements of copper at 5-8mg/kg, but since 1945 find that high-copper can improve the growth performance of pig significantly, all adopt the high-copper (in copper ion) of 200-250ppm (quality) at present in children's piglet stage in age, high-copper has the effect of the secretion of solidifying protein, the propagation that reduces microorganism, the peptide Y (NPY) that excites nerve, thereby promote that animal searches for food, improve digestive enzyme activity, finally improve the growth performance effect.So pig has become many nutritionists " custom " so far in the 1950's with high-copper.
At present, the source master of pig starter feed high-copper is inorganic copper sources such as copper sulphate, basic copper chloride or basic copper sulfate, inorganic copper source itself water-soluble or its can be decomposed by hydrochloric acid in gastric juice in the pig stomach fast, thereby a large amount of free copper copper ions discharges fast under one's belt and is absorbed, thereby the amount of giving birth to that surpasses animal is limited the quantity of and caused toxic reaction.Yet in absorbed a large amount of free copper ion, have only a spot of copper ion can arrive the small intestine postmedian and play antimicrobial effect, thereby the copper ion that need add high concentration in feed just can play antibiotic somatotrophic effect.But, use high-copper to bring many side effects, mainly show as: (1) high-copper is to the infringement of body internal organs, use the 200ppm high-copper can cause the liver cell enlargement, the cytoplasmic granule sex change, the glomerulus enlargement, renal tubule swelling, capsula glomeruli oozes out albumen, the cardiac muscle fibre granular degeneration, and lymphocyte reduces under the spleen, the smooth muscle fibers hyperplasia, by evidence the high-copper weanling pig that the low copper of the comprehensive sick ratio of dermatitis ephrosis takes place is high more than 5%, illustrate that high-copper is one of inducement of the scorching nephrotic syndrome of pigskin, also be one of reason that causes the decline of pig resistance; (2) high-copper residual exceeding standard in organ influences the security of food.The copper sanitary standard (GB15199-94) of limiting the quantity of is no more than 10mg/kg in China's meat product, but adds the 250ppm high-copper in the piglet basal diet, and copper content is 186.27mg/kg in the liver; (4) high-copper can disturb the absorption of other nutrition, causes pig hypovitaminosis as vitamin and grease in the high-copper energy oxidation feed, and thick unrest of fur and feed quality descend; High-copper antagonism iron, zinc absorb and cause hypoferric anemia and pachylosis, zinc deficiency symptom such as are taken off by hair; (4) high-copper also has huge environmental pressure, and in ten thousand pig farms, annual compound of discharging copper reaches 2.5 tons, and high-copper poultry pollution underground water source makes fresh-water fishes death, and melon and fruit, vegetable crop is sterile or yield poorly.
Summary of the invention
The objective of the invention is to overcome that employed inorganic copper source provides the application of a kind of cupric glutamate or derivatives thereof as animal feed additive for promoting growth as the weak point of animal feed additive for promoting growth in the prior art.
Purpose of the present invention is achieved through the following technical solutions: a kind of cupric glutamate or derivatives thereof is as the application of animal feed additive for promoting growth.
Described cupric glutamate comprises: glutamic acid and copper 2: 1 in molar ratio or the chelate that forms at 1: 1.
Described derivative comprises:
(1) cupric glutamate and the different sour different salt that form are as the hydrochloride of cupric glutamate, the phosphate of cupric glutamate and the sulfate of cupric glutamate etc.;
(2) glutamic acid becomes inner complex salt or the chelate that forms with copper ion behind the monoesters;
(3) chelate or the salt of N-carbamylglutamic acid and different salt, acid amides and copper ion formation;
(4) with the cupric glutamate be the different shiff alkali that the basis forms;
(5) with the cupric glutamate be the different amide compounds that the basis forms;
(6) with the dipeptides of a kind of formation in glutamic acid and the amino acid and the chelate of copper ion formation;
(7) chelate or the salt of the formation of glutamine and copper ion.
Described animal comprises various cultivated animals, as: various artificial feeding animals such as pig, chicken, duck, goose, beef cattle, milk cow, sheep, various fish and shrimp, fox, ermine, racoon dog.
Described cupric glutamate or derivatives thereof is applied to the different growth phases of animal as the application of animal feed additive for promoting growth.
When described animal feed is perfect compound feed, add cupric glutamate as growth promoter, the consumption of cupric glutamate is in copper, and its content is 20～100ppm.
The present invention has following advantage and effect with respect to prior art: the present invention finds the effect of cupric glutamate or derivatives thereof as animal feed additive for promoting growth first.The present invention finds that cupric glutamate has low aqueous solubility, stronger acidproof ability and high bioavilability, with cupric glutamate as feed additive for promoting growth, have higher security and antibacterial activity than other copper source (comprising copper sulphate, basic copper chloride and cupric glycinate etc.), the addition of copper ion can reduce 60～80%, can reduce production costs and reduce pollution to environment, reduce the toxic and side effect of high-copper to animal simultaneously.
The specific embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
Embodiment 1
(1) synthetic cupric glutamate:
The preparation of A, 10% monosodium glutamate solution: take by weighing 100 gram sodium glutamates (chemical pure) and be dissolved in the 1000ml water;
The preparation of B, 13.37% copper-bath: accurately smart amount 133.7 gram cupric sulfate pentahydrates (chemical pure) are dissolved in the 1000ml water;
C, each 1000ml of 10% monosodium glutamate solution and 13.37% copper-bath is mixed, follow the pH value to 7.0 of regulating aforementioned mixed solution with NaOH solution, stirring at room reaction 2h, centrifugal, get precipitation, water suspended centrifugal washing precipitation 3 times is then dried to constant weight for 100 ℃, obtain the 117.4g cupric glutamate, yield is 96.31%.
(2) glutamic acid of the cupric glutamate that step (1) is obtained and the content of copper ion are measured respectively:
The mensuration of A, content of copper ion: accurately take by weighing the 1.0g cupric glutamate, add water 80ml, use 0.1M HCl adjust pH to 2.0 then, be stirred to cupric glutamate and dissolve fully, with the content of copper ion in the atom absorption method mensuration solution, recording copper ion concentration is 2.807mg/ml.
The assay of B, glutamic acid: accurately take by weighing 1.0 gram cupric glutamates, add water 80ml, use 0.1MHCl adjust pH to 2.0 then, be stirred to cupric glutamate all after the dissolving, with the content of amino-acid analyzer mensuration glutamic acid, the content that records glutamic acid is 6.404mg/ml.
Thereby the mol ratio of copper ion and glutamic acid is 1: 1 in definite cupric glutamate, and crystallization water content is 7.89% in the cupric glutamate, and therefore, the molecular formula of the cupric glutamate that step (1) obtains is C
5H
8NO
4CuH
2O, molecular weight are 228.
(3) the antibacterial activity in vitro research of the cupric glutamate that obtains of step (1)
1. test material
A, culture medium: LB fluid nutrient medium: the 10g tryptone, 5g yeast extract and 10g NaCl are dissolved in the 800ml distilled water, with being settled to 1000mL behind the 1M NaOH adjust pH to 7.4, autoclaving 20min;
B, bacterial strain: e. coli jm109, salmonella typhimurium 50772 and staphylococcus aureus PNB14, all preserve the center available from China Veterinary Drugs Supervisory Inst.'s bacterial classification;
C, test tube: 10ml teat glass with cover;
D, medicine: CuSO
45H
2O solution: 0.2% (in copper ion, the mass/volume percent concentration, down together); Basic copper chloride (Cu
2Cl. (OH)
3): 0.2% (, using the 0.01M dissolving with hydrochloric acid) in copper ion; Cupric glutamate solution: 0.2% (with the DMSO dissolving, in copper ion);
2. test method: determination of tube method copper sulphate, basic copper chloride and cupric glutamate are to the antibacterial activity of e. coli jm109, salmonella typhimurium 50772 and staphylococcus aureus PNB14:
A, establish 9 groups, every group comprises 12 sterile test tube, numbering 1～12; Every big group establish three parallel, promptly comprise three groups;
Under B, the aseptic condition, add 2.0ml LB fluid nutrient medium to the 1 to 11 pipe respectively;
C, the copper sulphate with preparing, basic copper chloride or cupric glutamate solution to be checked add 2.0ml to the 1 pipe respectively, 2.0ml to the 3 pipes will be got after the 1st pipe mixing, to the 10th pipe, get 2.0 milliliters from the 10th pipe again and lose successively, the 11st pipe is made positive control for not adding antiradiation drug;
C, prepare LB Liquid Culture parent tube (the 12nd pipe) 2.0ml in addition and do not add medicine and bacterium, as negative control;
The 1-11 pipe of D, every big group adds e. coli jm109, salmonella typhimurium 50772 and the staphylococcus aureus PNB14 for test respectively, and (concentration of bacterium is about 10 respectively to every pipe 5.0 microlitre bacterium liquid
8Cfu/ml, cell age is 16-18 hour);
E, 37 ℃ of static cultivations 16 hours, perusal has or not bacterial growth, and last pipe Chinese traditional medicine concentration that bacterial growth do not occur promptly is the minimal inhibitory concentration (μ g/ml) of medicine to corresponding bacterium; Positive control is answered the visible haze growth, and negative control should be clarified.
3. result of the test:
In copper ion, copper sulphate and basic copper chloride are 500 μ g/ml, 500 μ g/ml and 500 μ g/ml (in copper ion) to the minimal inhibitory concentration of e. coli jm109, salmonella typhimurium 50772 and staphylococcus aureus PNB14, and cupric glutamate to the minimal inhibitory concentration of e. coli jm109, salmonella typhimurium 50772 and staphylococcus aureus PNB14 for being respectively 125 μ g/ml, 125 μ g/ml and 125 μ g/ml (in copper ion), antibacterial activity has improved 4 times.
The antibacterial activity comparative studies result of table 1 copper sulphate and cupric glutamate
(4) (rat LD is measured in the security of the cupric glutamate that obtains of step (1)
50Measure, improve karber's method)
1. test material
Rat: available from Nanfang Medical Univ's Experimental Animal Center, body weight 120～150 grams, male and female half and half.
Supply test specimen: cupric sulfate pentahydrate, basic copper chloride and cupric glutamate
Equipment: syringe, filling stomach syringe needle, mouse cage
2. test method
A, obtain causing animal 0% (Dn) and the dead dosage of 100% (Dm) by preliminary experiment, wherein the Dn of cupric sulfate pentahydrate, basic copper chloride and cupric glutamate and Dm see Table 2.
Table 2 different Cu source compound rat LD50 trial test result (Dn and Dm)
| Compound | Dn (mg/kg body weight) | Dm (mg/kg body weight) |
| Cupric sulfate pentahydrate | ??1000 | ??1800 |
| Basic copper chloride | ??1200 | ??2000 |
| Cupric glutamate | ??10000 | ??15000 |
The maximum reactivity of B, this requirement of experiment is 100%, and the minimal reaction rate is 0%, or at least reactivity near 100% or 0%; Dn and the Dm value of dose ratio according to above-mentioned each compound on average is provided with 8 dosage between group between Dn to Dm, every group of 10 rats.When 100% and 0% reactivity of repetition being arranged, the group of keeping to the side should be discarded and disregard as adjacent doses occurring in the experiment, the reactivity that makes heavy dose of group have only 100%, small dose group also has only one 0% reactivity; After grouping finished, grouping gavaged copper sulphate, basic copper chloride or the cupric glutamate of various dose; Observed 7 days after the administration, viewing duration writes down the toxic reaction situation of animal and the distribution of dead animal day by day
C, account form are respectively organized the death rate by LD50 and the fiducial limit (P=0.95) of following formula different Cu source compound to rat according to formal experiment
A, when the death rate of minimum dose group is 0%, the death rate of maximum dose group is 100% o'clock, calculates LD50 by following formula:
LD
50＝lg
-1[Xm-i(∑p-0.5)]
B, when the death rate of minimum dose group greater than 0% and less than 30%, or the death rate of maximum dose group can be calculated LD by following updating formula less than 100% and greater than 70% o'clock
50:
LD
50Standard error:
LD
50Average fiducial limit: LD
50± 4.5 S
X50LD
50(P=0.95);
In the following formula, Xm is the logarithm of maximum dose group dosage, and i is the difference (heavy dose of group reduces the dosage group) of two adjacent groups log10 dose, and Pm is the maximum dose group death rate, and Pn is the minimum dose group death rate, and P is each group death rate, and n is every treated animal number.
D, result of the test
In copper ion, rat sees Table 3 to the dead dosage of oral half (LD50) of all given the test agent, and the result shows that cupric glutamate improves respectively about 10 times and 4 times than copper sulphate and the right security of basic copper sulfate.
Table 3 copper sulphate, basic copper chloride and cupric glutamate are to the oral median lethal dose result of study of rat
| Given the test agent | LD50 (the mg/kg body weight is in compound) | LD50 (the mg/kg body weight is in copper ion) |
| Cupric sulfate pentahydrate | ??1300 | ??332.8 |
| Basic copper chloride | ??1500 | ??900 |
| Cupric glutamate | ??12000 | ??3383.3 |
(5) mensuration of the cupric glutamate equilbrium solubility that obtains of step (1)
1. test material
Potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate, sodium hydrogen phosphate, NaOH, hydrochloric acid, phosphoric acid, cupric sulfate pentahydrate and basic copper chloride etc. are chemically pure reagent.
2. test method
The preparation of A, buffer solution: secure ph be respectively 2.0,3.0,4.0,5.0,6.0,7.0 phosphate buffer (PBS, 0.1M).
The mensuration of B, equilbrium solubility: getting cupric glutamate, to add the pH value respectively be in 2.0,3.0,4.0,5.0,6.0,7.0 the 100ml PBS solution, 60 ℃ of water-baths heating is also ultrasonicly no longer dissolved to cupric glutamate, put into water bath chader, temperature keeps (37 ± 1) ℃, jolting 24h; After saturated solution centrifugation,, calculate the equilbrium solubility of cupric glutamate with the copper ion in the aas determination solution; Same procedure is measured the equilbrium solubility of cupric sulfate pentahydrate, basic copper chloride.
3. result of the test
The result shows that cupric sulfate pentahydrate is soluble in water, and basic copper chloride is water insoluble, and is easily molten under the acid condition, and glutamic acid ketone is water-soluble low, and acidproof endurance strong (table 4).
The equilbrium solubility of table 4 cupric glutamate, copper sulphate and basic copper chloride
(6) the profit distribution coefficient of the cupric glutamate that obtains of step (1) is measured
1. test material: n-octyl alcohol, cupric glutamate, cupric sulfate pentahydrate, basic copper chloride
2. test method is an example with the cupric glutamate, and cupric sulfate pentahydrate, basic copper chloride are equally in order to method detection down
A, cupric glutamate are measured in the equilbrium solubility of n-octyl alcohol solution: take by weighing 5 gram cupric glutamates, add 100 milliliters of n-octyl alcohol solution, 60 ℃ of water-baths heating is also ultrasonicly no longer dissolved to cupric glutamate, puts into water bath chader, temperature keeps (37 ± 1) ℃, jolting 24h; After saturated solution centrifugation, the copper ion with in the aas determination solution calculates the equilbrium solubility of cupric glutamate in n-octyl alcohol solution.
B, vortex time are investigated: getting mass concentration is 400mgL
-1Cupric glutamate n-octyl alcohol solution (by water saturated after n-octyl alcohol) 0.5mL, add by n-octyl alcohol the 0.1molL after saturated
-1Hydrochloric acid solution 5ml, difference vortex 5,10,20,45,60min, 10000rmin
-1Centrifugal 2min gets the n-octyl alcohol layer is measured copper ion in the n-octyl alcohol with atom absorption method content; Determine the suitableeest vortex time.
C, profit distribution coefficient are measured: n-octyl alcohol solution (saturated by the water) 0.5mL that gets cupric glutamate adds entry-cushioning liquid (saturated by n-octyl alcohol) 5ml as water as oil phase, vortex min (result according to step 2 is fixed), 10000rmin
-1Centrifugal 2min, the oil phase 0.1ml that gets the upper strata measures copper ion concentration with atom absorption method, calculates the distribution situation of cupric glutamate at oil phase and aqueous phase, calculates profit and divides distribution coefficient (P)=Po/Pw.
3. result of the test
Solubility copper sulphate in water is the highest, and basic copper chloride takes second place, and cupric glutamate is minimum.The solubility of three's solubility copper sulphate is the highest in n-octyl alcohol solution, and cupric glutamate takes second place, and basic copper chloride is minimum.The profit distribution coefficient that calculates each compound is respectively: cupric glutamate 0.0102, basic copper chloride 0.001, and copper sulphate has only 0.0002.Cupric glutamate is than the antibacterial activity of basic copper chloride and copper sulphate high 10 times and 50 times (table 5), and profit divides the system of disposition high more, compound fat-soluble strong more, and antibacterial activity is then strong more.
The profit distribution coefficient of table 5 cupric glutamate, copper sulphate and basic copper chloride
(6) the growth promotion effect test of the cupric glutamate that obtains of step (1)
1. test material
Experimental animal: 350 the 1 yellow fast big fryer (herding research institute of guangdong agricultural science institute) in the age in days south of the Five Ridges; 50 weanling pigs (Guangdong the people live in plenty plant the pig farm);
Feed: the 102 type chickens that do not contain any antibacterials are used perfect compound feed, and Animal Husbandry Inst., Guangdong Prov. Academy of Agricultural Sciences's feed factory is special; The 303 type pigs that do not contain any antibacterials are used perfect compound feed, and the people live in plenty in Guangdong, and herding Development Co., Ltd feed factory is special;
Given the test agent: cupric glutamate, cupric sulfate pentahydrate.
2. test method
A, cupric glutamate are tested the growth promotion of the yellow fast big fryer in the south of the Five Ridges
350 the 1 yellow fast large-scale fryer in the age in days south of the Five Ridges are divided into 7 groups, 50 every group at random.Each group is added different growth promoters (table 6) in feed after, free choice feeding, the weightening finish and the price of deed of each test group test chicken of statistics 1～21 age in days, and relatively copper sulphate and cupric glutamate to the growth enhancing effect of fryer.
Table 6 cupric glutamate is to the growth promotion test grouping of fryer
| Group | Size of animal | Average initial weight (g) | Growth promoter | Concentration (ppm) * |
| Do not add short long agent control group | ??50 | ?50 | ??- | ??- |
| Cupric sulfate pentahydrate-150 | ??50 | ?50 | Cupric sulfate pentahydrate | ??150 |
| Group | Size of animal | Average initial weight (g) | Growth promoter | Concentration (ppm) * |
| Cupric sulfate pentahydrate-100 | ??50 | ?50 | Cupric sulfate pentahydrate | ??100 |
| Cupric sulfate pentahydrate-50 | ??50 | ?50 | Cupric sulfate pentahydrate | ??50 |
| Cupric glutamate-150 | ??50 | ?50 | Cupric glutamate | ??150 |
| Cupric glutamate-100 | ??50 | ?50 | Cupric glutamate | ??100 |
| Cupric glutamate-50 | ??50 | ?50 | Cupric glutamate | ??50 |
*: the working concentration of different growth promoters is in the copper ion in the compound.
B, cupric glutamate are tested the growth promotion of pig
60 weanling pigs such as table 7 grouping, 10 every group.Each group is added different growth promoters (seeing Table 7) in feed after, free choice feeding, the weightening finish and the price of deed of back 30 days each test group test pig of statistics wean, and relatively copper sulphate and cupric glutamate to the growth enhancing effect of pig.
Table 7 cupric glutamate is to the growth promotion test grouping of pork pig
| Group | Size of animal | Average initial weight (kg) | Growth promoter | Concentration (ppm) * |
| Do not add short long agent control group | ??10 | ??8.40 | ??- | ??- |
| Cupric sulfate pentahydrate-250 | ??10 | ??8.45 | Cupric sulfate pentahydrate | ??250 |
| Cupric glutamate-100 | ??10 | ??8.52 | Cupric glutamate | ??100 |
| Cupric glutamate-75 | ??10 | ??8.48 | Cupric glutamate | ??75 |
| Cupric glutamate-50 | ??10 | ??8.25 | Cupric glutamate | ??50 |
| Cupric glutamate-30 | ??10 | ??8.36 | Cupric glutamate | ??30 |
*: the working concentration of different growth promoters is in the copper ion in the compound.
3. result of the test
A, cupric glutamate are tested the growth promotion of the yellow fast big fryer in the south of the Five Ridges
In the feeding experiment process of the yellow fast big fryer in the south of the Five Ridges, the copper sulphate group especially copper ion 150ppm and 100ppm group in second week of test, poisoning symptoms such as feather is in disorder, skin is dry appear in the yellow fast big fryer in the south of the Five Ridges, a small amount of dead chicken phenomenon before off-test, also occurs, illustrate that copper sulphate is added on the medium-term and long-term use of feed at 100ppm and the copper ion poisoning can occur.Though clinical visible poisoning symptom does not appear in the copper sulphate group of 50ppm copper ion, the relative weight gain rate of experimental period is 99.6%, and is approaching with not administration control group, but feedstuff-meat ratio than the control group height 0.052.3 dosage groups of cupric glutamate all show good growth promoting function, and the relative weight gain rate can improve 6.3-7.3%, the price of deed 0.054-0.071 (seeing table 8 for details) that can descend.Security that growth test results suggest cupric glutamate tool is good and growth promotion effect.
Table 8 cupric glutamate is to the growth promotion result of the test of fryer
| Group | Size of animal (only) | Survival rate (%) | Average weight gain (g) | The relative weight gain rate (%) | Total augment weight (kg) | Total feed consumption (kg) | Feedstuff-meat ratio |
| Not dosing control group | ??50 | ??100 | ??478 | ??- | ??23.90 | ??44.29 | ??1.853 |
| Copper sulphate-150 | ??50 | ??90 | ??446 | ??93.3 | ??20.80 | ??42.54 | ??2.045 |
| Copper sulphate-100 | ??50 | ??96 | ??462 | ??96.7 | ??22.84 | ??43.95 | ??1.924 |
| Copper sulphate-50 | ??50 | ??100 | ??476 | ??99.6 | ??23.80 | ??45.34 | ??1.905 |
| Cupric glutamate-150 | ??50 | ??100 | ??513 | ??107.3 | ??25.65 | ??45.79 | ??1.785 |
| Cupric glutamate-100 | ??50 | ??100 | ??509 | ??106.5 | ??25.45 | ??45.35 | ??1.782 |
| Cupric glutamate-50 | ??50 | ??100 | ??508 | ??106.3 | ??25.40 | ??45.69 | ??1.799 |
B, cupric glutamate are tested the growth promotion of pig
In the swine rearing process of the test, the cupric sulfate pentahydrate group (250ppm) of high dose has tangible growth promoting function, and flat more not administration of the counterpoise control group that increases of duration of test improves 7.4%, and the price of deed reduces by 0.177.The cupric glutamate of various dose group all shows the growth enhancing effect with high dose copper sulphate, and the growth promotion effect close (table 9) of 50ppm and 100ppm and 150ppm dosage group, and the cupric glutamate of results suggest 50ppm can replace the copper sulphate of 250ppm.
Table 9 cupric glutamate is to the growth promotion result of the test of pig
| Group | Size of animal (only) | Survival rate (%) | Average weight gain (kg) | The relative weight gain rate (%) | Total augment weight (kg) | Total feed consumption (kg) | Feedstuff-meat ratio |
| Not dosing control group | ??10 | ??100 | ??11.20 | ??100 | ??112 | ??251.33 | ??2.244 |
| Cupric sulfate pentahydrate-250 | ??10 | ??100 | ??12.28 | ??109.65 | ??122.8 | ??253.82 | ??2.067 |
| Cupric glutamate-100 | ??10 | ??100 | ??12.36 | ??110.36 | ??123.6 | ??252.76 | ??2.045 |
| Cupric glutamate-75 | ??10 | ??100 | ??12.18 | ??108.75 | ??121.8 | ??254.68 | ??2.091 |
| Cupric glutamate-50 | ??10 | ??100 | ??11.92 | ??106.43 | ??119.2 | ??250.08 | ??2.098 |
| Cupric glutamate-30 | ??10 | ??100 | ??11.45 | ??102.24 | ??114.5 | ??244.0 | ??2.131 |
Embodiment 2
The cupric glutamate derivative is to the growth promotion effect test of animal
(1) preparation of the derivative of different cupric glutamates
A, cupric glutamate hydrochloride: take by weighing 100 gram CuSO45H
2O and 74.8 gram sodium glutamates are dissolved in respectively in the 1000ml water, stir to dissolving fully, and the two is mixed, and with the 0.1M hydrochloric acid solution pH value of mixed solution are transferred to 4.0, and heating and continuous stirring are reacted, and gather in the crops crystallization and dry to constant weight.Get product and be dissolved in water with 1 gram and be settled to 100ml, measure the content of copper ion (atomic absorption method), chlorion (Moire technique) and glutamic acid (amino-acid analyzer) in the solution.Copper ion concentration is 2.607mg/ml as a result, and chloride ion content is 1.446mg/ml, and the content of glutamic acid is 5.908mg/ml, and the crystal that can infer gained thus is the cupric glutamate hydrochloride that cupric glutamate and hydrochloric acid formed in 1: 1 in molar ratio.
The preparation of B, glutamic acid methyl ester copper chelate: take by weighing 100 gram CuSO45H
2O and 129.0 gram glutamic acid methyl esters are dissolved in respectively in the 1000ml water, stir to dissolving fully, with the two mixing, with 1M sodium hydroxide solution seasoning pH value to 7.0, centrifugal collecting precipitation, and behind the water centrifuge washing three times 100 ℃ dry to constant weight.Get and be settled to 100ml water, the content of copper ion (atomic absorption method) and glutamic acid (amino-acid analyzer) in the mensuration solution after 1 gram product dissolves fully with 0.01M HCl solution.Copper ion concentration is 1.592mg/ml as a result, and the content of glutamic acid is 7.314mg/ml, and the product that can infer gained thus is the chelate of band a part crystallization water of being formed in 2: 1 in molar ratio by glutamic acid methyl ester and copper ion.
The preparation of C, N-carbamylglutamic acid copper: take by weighing 100 gram CuSO45H
2O and 76.8 gram N-carbamylglutamic acids are dissolved in respectively in the 1000ml water, stir to dissolving fully, with the two mixing, with 1M sodium hydroxide solution seasoning pH value to 7.0, centrifugal collecting precipitation, and behind the water centrifuge washing three times 100 ℃ dry to constant weight.Get and be settled to 100ml water, the content of copper ion (atomic absorption method) and glutamic acid (amino-acid analyzer) in the mensuration solution after 1 gram product dissolves fully with 0.01M HCl solution.Copper ion concentration is 2.215mg/ml as a result, and the content of glutamic acid is 5.087mg/ml, and the product that can infer gained thus is the chelate of band a part crystallization water of being formed in 1: 1 in molar ratio by N-carbamylglutamic acid and copper ion.
The preparation of D, glutamine copper: take by weighing 100 gram CuSO45H
2O and 116.8 gram glutamine are dissolved in respectively in the 1000ml water, stir to dissolving fully, with the two mixing, with 1M sodium hydroxide solution seasoning pH value to 7.0, centrifugal collecting precipitation, and behind the water centrifuge washing three times 100 ℃ dry to constant weight.Get and be settled to 100ml water, the content of copper ion (atomic absorption method) and glutamine (amino-acid analyzer) in the mensuration solution after 1 gram product dissolves fully with 0.01M HCl solution.Copper ion concentration is 1.721mg/ml as a result, and the content of glutamic acid is 7.850mg/ml, and the product that can infer gained thus is the chelate of band a part crystallization water of being formed in 2: 1 in molar ratio by N-carbamylglutamic acid and copper ion.
The preparation of E, lysyl cupric glutamate: take by weighing 100 gram CuSO45H
2O and 110.0 gram lysyl glutamic acid are dissolved in respectively in the 1000ml water, stir to dissolving fully, with the two mixing, with 1M sodium hydroxide solution seasoning pH value to 7.0, centrifugal collecting precipitation, and behind the water centrifuge washing three times 100 ℃ dry to constant weight.Get and be settled to 100ml water after 1 gram product dissolves fully with 0.01M HCl solution, measure the content of copper ion (atomic absorption method), lysine and glutamic acid (amino-acid analyzer) in the solution.Copper ion concentration is 1.803mg/ml as a result, lysine content 4.113mg/ml, the content of glutamic acid is 4.141mg/ml, and the product that can infer gained thus is the integration thing of band a part crystallization water of being formed in 1: 1 in molar ratio by lysyl glutamic acid and copper ion.
The preparation of F, salicylide cupric glutamate (cupric glutamate shiff alkali): 0.02mmol potassium hydroxide is dissolved in the 1000mL distilled water. and be added drop-wise to 500mL and contain in 0.02mmol sodium glutamate and the 0.02mmol salicylide ethanolic solution, under 80 ℃ of water-baths, stirring reaction 2h adds 1000mL then and contains 0.02mmol CuSO45H
2The O aqueous solution is regulated pH to 7.0, continues reaction 8h, and cold filtration is collected heavy and dried to constant weight.Get and be settled to 100ml water, the content of copper ion (atomic absorption method) and glutamic acid (amino-acid analyzer) in the mensuration solution after 1 gram product dissolves fully with 0.01M HCl solution.Copper ion concentration is 1.835mg/ml as a result, and the content of glutamic acid is 4.200mg/ml, and the product that can infer gained thus is the chelate of band a part crystallization water of being formed in 1: 1 in molar ratio by salicylide glutamic acid and copper ion.
(2) the cupric glutamate derivative is to the promoting animal growth Research on effect
1. test material
Experimental animal: 90 weanling pigs: the people live in plenty in Guangdong plants the pig farm provides;
Feed: the 303 type pigs that do not contain any antibacterials are used perfect compound feed, and the people live in plenty in Guangdong, and herding Development Co., Ltd feed factory is special;
Given the test agent: six kinds of cupric glutamate derivatives (cupric glutamate hydrochloride, glutamic acid methyl ester copper chelate, N-carbamylglutamic acid copper, glutamine copper, lysyl cupric glutamate and salicylide cupric glutamate), the CuSO45H of cupric glutamate, step (1) preparation
2O.
2. test method
90 weanling pigs such as table 9 grouping, 10 every group.Each group is added different growth promoters (seeing Table 10) in feed after, free choice feeding, the weightening finish and the price of deed of back 30 days each test group test pig of statistics wean, and relatively copper sulphate and cupric glutamate or derivatives thereof to the difference of the growth enhancing effect of pig.
The different cupric glutamate derivatives of table 10 are to the growth promotion test grouping of pork pig
| Group | Size of animal | Average initial weight (kg) | Growth promoter | Concentration (ppm) * |
| ??1 | ??10 | ??8.32 | ??- | ??- |
| ??2 | ??10 | ??8.35 | Cupric sulfate pentahydrate | ??250 |
| ??3 | ??10 | ??8.42 | Cupric glutamate | ??50 |
| ??4 | ??10 | ??8.40 | The cupric glutamate hydrochloride | ??50 |
| ??5 | ??10 | ??8.45 | The glutamic acid methyl ester copper chelate | ??50 |
| ??6 | ??10 | ??8.46 | Cupric glutamate | ??50 |
| ??7 | ??10 | ??8.30 | N-carbamylglutamic acid copper | ??50 |
| ??8 | ??10 | ??8.35 | The lysyl cupric glutamate | ??50 |
| ??9 | ??10 | ??8.36 | The salicylide cupric glutamate | ??50 |
*: the working concentration of different growth promoters is in the copper ion in the compound.
3. the cupric glutamate derivative is to the growth promotion test result of the test of pork pig
Result of the test among the embodiment 1 has confirmed that there is suitable growth promoting function in the cupric glutamate copper source of 50ppm and the cupric sulfate pentahydrate copper source of 250ppm.The cupric sulfate pentahydrate copper source (group 2) of the cupric glutamate copper source of 50ppm in the present embodiment (group 3) and 250ppm has improved 11.36% and 10.63% respectively than the average weight gain of control group, feedstuff-meat ratio has also reduced by 0.273 and 0.261 respectively, has reconfirmed that the cupric glutamate copper source of 50ppm in the feed process for preparation can substitute the cupric sulfate pentahydrate copper source of 250ppm.In addition, the various derivatives of cupric glutamate and paddy ammonia copper have suitable growth promotion effect (table 11), and the derivative of results suggest cupric glutamate and cupric glutamate have suitable biological effect.
The different cupric glutamate derivatives of table 11 are to the growth promotion result of the test of pig
| Group | Size of animal (only) | Survival rate (%) | Average weight gain (kg) | The relative weight gain rate (%) | Total augment weight (kg) | Total feed consumption (kg) | Feedstuff-meat ratio |
| ??1 | ??10 | ??100 | ??11.01 | ??100 | ??110.1 | ??266.2 | ??2.418 |
| ??2 | ??10 | ??100 | ??12.18 | ??110.63 | ??121.8 | ??262.7 | ??2.157 |
| ??3 | ??10 | ??100 | ??12.26 | ??111.36 | ??122.6 | ??263 | ??2.145 |
| ??4 | ??10 | ??100 | ??12.18 | ??110.63 | ??121.8 | ??265.6 | ??2.181 |
| ??5 | ??10 | ??100 | ??12.22 | ??110.99 | ??122.2 | ??267.4 | ??2.188 |
| ??6 | ??10 | ??100 | ??12.45 | ??113.08 | ??124.5 | ??266.5 | ??2.146 |
| ??7 | ??10 | ??100 | ??12.28 | ??111.54 | ??122.8 | ??266.1 | ??2.167 |
| ??8 | ??10 | ??100 | ??12.06 | ??109.54 | ??120.6 | ??258.7 | ??2.145 |
| ??9 | ??10 | ??100 | ??12.38 | ??112.45 | ??123.8 | ??260.1 | ??2.101 |
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (6)
1. the cupric glutamate or derivatives thereof is as the application of animal feed additive for promoting growth.
2. according to the application of the described cupric glutamate or derivatives thereof of claim 1 as animal feed additive for promoting growth, it is characterized in that: described cupric glutamate is the chelate of glutamic acid and copper 2: 1 in molar ratio or formation in 1: 1.
3. according to the application of the described cupric glutamate or derivatives thereof of claim 1 as animal feed additive for promoting growth, it is characterized in that: described derivative comprises:
(1) cupric glutamate and the sour salt that forms;
(2) glutamic acid becomes inner complex salt or the chelate that forms with copper ion behind the monoesters;
(3) chelate or the salt of N-carbamylglutamic acid and salt, acid amides and copper ion formation;
(4) with the cupric glutamate be the shiff alkali that the basis forms;
(5) with the cupric glutamate be the amide compound that the basis forms;
(6) with the dipeptides of a kind of formation in glutamic acid and the amino acid and the chelate of copper ion formation;
(7) chelate or the salt of the formation of glutamine and copper ion.
4. according to the application of the described cupric glutamate or derivatives thereof of claim 1 as animal feed additive for promoting growth, it is characterized in that: described animal is a cultivated animals.
5. according to the application of the described cupric glutamate or derivatives thereof of claim 1 as animal feed additive for promoting growth, it is characterized in that: described cupric glutamate or derivatives thereof is applied to each growth phase of animal.
6. according to of the application of the described cupric glutamate or derivatives thereof of claim 1 as animal feed additive for promoting growth, it is characterized in that: when described feed is perfect compound feed, add cupric glutamate as growth promoter, with feed weight is benchmark, the consumption of cupric glutamate is 20～100ppm in the copper molal quantity.
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| CN2010101226257A CN101756042B (en) | 2010-03-12 | 2010-03-12 | Application of cupric glutamate or derivatives thereof as feed additive for promoting animal growth |
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| CN2010101226257A CN101756042B (en) | 2010-03-12 | 2010-03-12 | Application of cupric glutamate or derivatives thereof as feed additive for promoting animal growth |
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| CN2012100755394A Division CN102599347A (en) | 2010-03-12 | 2010-03-12 | Application of cupric glutamate or derivate thereof being taken as animal growth promotion feed additive |
| CN2012100750070A Division CN102578393A (en) | 2010-03-12 | 2010-03-12 | Use of cupric glutamate or derivative thereof as growth-promoting feed additive for animals |
| CN201210075538XA Division CN102578394A (en) | 2010-03-12 | 2010-03-12 | Application of cupric glutamate or derivative of cupric glutamate serving as feed additive for promoting animal growth |
| CN201210075009XA Division CN102599346A (en) | 2010-03-12 | 2010-03-12 | Application of cupric glutamate or derivative of cupric glutamate as animal growth promotion feed additive |
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| CN101756042B CN101756042B (en) | 2012-07-04 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106473A (en) * | 2010-12-07 | 2011-06-29 | 北京龙科方舟生物工程技术中心 | Feed containing mixture of N-carbamylglutamic acid and sodium salt thereof for boars, preparation and application thereof |
| CN102627583A (en) * | 2012-03-22 | 2012-08-08 | 广州九益生物技术有限公司 | N-Carbamyl-L-glutamic acid copper complex and its preparation method |
| CN102718685A (en) * | 2012-06-01 | 2012-10-10 | 广州九益生物技术有限公司 | Preparation method and application of N-carbamyl-L-glutamic acid manganese complex |
| CN105519799A (en) * | 2016-01-22 | 2016-04-27 | 广州先至饲料添加剂有限公司 | Application of copper calcium sulphate as animal fodder additive |
| CN107960533A (en) * | 2016-10-20 | 2018-04-27 | 湖南晶天科技实业有限公司 | A kind of amino acid aldehyde schiff bases feed addictive and a kind of feed |
| CN113170837A (en) * | 2021-04-07 | 2021-07-27 | 彭险峰 | Copper aspartate complex and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1668577A (en) * | 2002-10-16 | 2005-09-14 | 金宝公司 | Metal complexes of alpha amino dicarboxylic acids |
| CN1720249A (en) * | 2002-12-05 | 2006-01-11 | Md白奥阿尔法有限公司 | Method for preparation of amino acid chelate |
| CN101076256A (en) * | 2004-02-26 | 2007-11-21 | 塔斯克制品知识产权控股公司 | Antimicrobial food additive and prepared food, water and waste water treatment |
-
2010
- 2010-03-12 CN CN2010101226257A patent/CN101756042B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1668577A (en) * | 2002-10-16 | 2005-09-14 | 金宝公司 | Metal complexes of alpha amino dicarboxylic acids |
| CN1720249A (en) * | 2002-12-05 | 2006-01-11 | Md白奥阿尔法有限公司 | Method for preparation of amino acid chelate |
| CN101076256A (en) * | 2004-02-26 | 2007-11-21 | 塔斯克制品知识产权控股公司 | Antimicrobial food additive and prepared food, water and waste water treatment |
Non-Patent Citations (1)
| Title |
|---|
| 《无机精细化学品的制备和应用》 19991031 熊家林等 氨基酸配合物 化学工业出版社 583 , 第一版 1 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106473A (en) * | 2010-12-07 | 2011-06-29 | 北京龙科方舟生物工程技术中心 | Feed containing mixture of N-carbamylglutamic acid and sodium salt thereof for boars, preparation and application thereof |
| CN102106473B (en) * | 2010-12-07 | 2014-04-02 | 北京龙科方舟生物工程技术有限公司 | Feed containing mixture of N-carbamylglutamic acid and sodium salt thereof for boars, preparation and application thereof |
| CN102627583A (en) * | 2012-03-22 | 2012-08-08 | 广州九益生物技术有限公司 | N-Carbamyl-L-glutamic acid copper complex and its preparation method |
| CN102718685A (en) * | 2012-06-01 | 2012-10-10 | 广州九益生物技术有限公司 | Preparation method and application of N-carbamyl-L-glutamic acid manganese complex |
| CN105519799A (en) * | 2016-01-22 | 2016-04-27 | 广州先至饲料添加剂有限公司 | Application of copper calcium sulphate as animal fodder additive |
| CN105519799B (en) * | 2016-01-22 | 2018-08-07 | 广州英赛特生物技术有限公司 | Application of the copper calcium sulfate as animal feed additive |
| CN107960533A (en) * | 2016-10-20 | 2018-04-27 | 湖南晶天科技实业有限公司 | A kind of amino acid aldehyde schiff bases feed addictive and a kind of feed |
| CN113170837A (en) * | 2021-04-07 | 2021-07-27 | 彭险峰 | Copper aspartate complex and application thereof |
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| CN101756042B (en) | 2012-07-04 |
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