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CN101581729B - Liquid sample processing device - Google Patents

Liquid sample processing device Download PDF

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Publication number
CN101581729B
CN101581729B CN2009100163258A CN200910016325A CN101581729B CN 101581729 B CN101581729 B CN 101581729B CN 2009100163258 A CN2009100163258 A CN 2009100163258A CN 200910016325 A CN200910016325 A CN 200910016325A CN 101581729 B CN101581729 B CN 101581729B
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China
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cavity
reagent
piston
packed
liquid sample
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Expired - Fee Related
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CN2009100163258A
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CN101581729A (en
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何峰
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Individual
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Abstract

The invention relates to a liquid sample processing device and belongs to the technical field of the structure of liquid sample processing devices. The liquid sample processing device is characterizedby comprising at least one cavity containing at least one reagent, wherein the lower part of the cavity is provided with at least one capillary tube communicated with the cavity; the inside of each c avity is provided with at least one sealing piston having a pusher arm which is arranged on the upper part; the lower part of each sealing piston having a pusher arm which is arranged on the upper part is correspondingly provided with at least one closed body which is used for isolating the reagent, completing the cavity with a guiding channel and mixing the sample and reagents through movement; the reagent is between the sealing piston and the closed body; and the guiding channel arranged in the cavity is used to realize the mixing of the reagent in the cavity and the sample in the capillary tube. The liquid sample processing device is reasonable in structure, simple and convenient in operation, applicable to various conditions, capable of simplifying and facilitating detection and used for real-time detection.

Description

Liquid sample processing device
One, technical field
The present invention relates to a kind of liquid sample processing device, particularly a kind of liquid sample collection, reagent store and with the two mix, the device of mixing, belong to the liquid sample processing device technical field of structures.
Two, background technology
(Point-of-Care Testing POCT), claims other check of bed or POCT again to real-time test, has the advantage that assay is offered immediately the doctor.And traditional laboratory detection need be delivered to the laboratory with the sample of gathering, and waits for that several hours time to several weeks could send the result to doctor.
Have only that operation steps is few, method of operating is simple, be easy to learn and use, and does not need the large automatic instrument to handle, and can become the real-time test project by the project of amateur docimaster such as nurse or patient oneself completion.
Large automatic instrument detecting sample is used in the laboratory basically now, need not artificial the intervention.Before self-reacting device was widely used, a lot of test items were accomplished by manual work, and such method is still being used in some small test chamber now.Manual detection sample complex steps needs to operate through the docimaster of special training.As detect the glucose in the blood; Drawing sample with micro sample adding appliance earlier joins in the reaction cup; Sample of every absorption need be changed the suction nozzle of sample injector to avoid cross pollution, draws the first component reagent in reaction cup with sample injector then, sways mixing; Draw the second component reagent with sample injector again and add in the above-mentioned reaction cup, detect with photometer after swaying mixing once more.Some detectable is a dry powder for another example, need add quantitative water or reagent before use and process working fluid and can use.Obviously so complicated operations has limited the application of a lot of test items in real-time test.
Three, summary of the invention
The objective of the invention is to solve the weak point that above-mentioned prior art exists, provide a kind of and can save numerous and diverse operation steps, make and detect the liquid sample processing device that becomes simple and easy to do and can be applied to real-time test.
The present invention realizes through following technical scheme:
Liquid sample processing device; Special character is to comprise that at least one internal storage has the cavity 4 of at least a reagent; Cavity 4 bottoms are provided with at least one and are communicated with kapillary 7 with it; Be provided with the packed-piston 2 of at least one top band pusher arm 1 in each cavity 4; The bottom correspondence of the packed-piston 2 of each top band pusher arm 1 be provided with at least one be used to isolate reagent, with cavity supporting belt water conservancy diversion channel, can be through motion with the obturator of sample and reagent mix, reagent is between packed-piston 2 and obturator, mixing between reagent and the kapillary 7 interior samples passed through water conservancy diversion channel realization set in the cavity 4 in the cavity 4;
Said cavity 4 bottoms are communicated with kapillary 7 through the bottom of the cone-shaped cavity 5 that is attached thereto;
It is liquid reagent that the said reagent 8 that is stored in the cavity 4 has at least a kind of, and it is stored between packed-piston 2 and the obturator, and other kinds reagent is dry powder or liquid; Store between reagent and the packed-piston air or other kinds gas such as nitrogen etc. are arranged.
Said obturator for that be used with the water conservancy diversion channel, that fit tightly with cavity 4 inwalls, be used to isolate reagent 8 and make it to seal the packed-piston 9 that is stored in the cavity 4; The water conservancy diversion channel is set at least 1 diversion trench 12 of cavity 4 lower cavity inwalls; When the packed-piston 2 of pusher arm 1 drive bottom pressed down, under pressure, the packed-piston 9 of bottom also moved down thereupon; When moving to diversion trench 12 places; Packed-piston 9 stops to move down, and the pusher arm 1 drive packed-piston on top continues to move down at this moment, and the reagent 8 between packed-piston 9 and the packed-piston 2 gets into kapillaries 7 injection reaction cup and the sample mixing of also passing through cone-shaped cavity 5 bottoms in the cone-shaped cavities 5 along diversion trench 12; Continue to promote pusher arm 1 this moment; Make packed-piston 2 continue to move down, until reagent 8 and air are injected reaction cup fully, can be at the reagent and the pressure of the process of air injection reaction cup fully with reagent and sample mixing;
Said obturator is the diaphragm seal 18 in cavity 4 bottom seals being used for of connecting reagent 8 sealings being stored in; The puncturing lever 16 of water conservancy diversion channel for inserting into the inner by cavity 4 tops, diaphragm seal 18 realizations that penetrate cavity 4 bottoms through moving down of puncturing lever 16 that mix of reagent and sample; During use; Move down earlier diaphragm seal 18 is punctured, realize being communicated with between cavity 4 and the cone-shaped cavity 5, promote pusher arm 1 more at this moment and press down by puncturing lever 16; Reagent 8 in the cavity 4 enters in the cone-shaped cavity 5 through the diaphragm seal 18 of breakage under pressure, and realization reagent mixes with sample;
Be provided with between said pusher arm 1 and cavity 4 outer walls and be used to the locking system 3 that makes packed-piston 2 be in lockup state; The bottom of cone-shaped cavity 5 has the kapillary 7 that is used to draw the quantitative liquid sample, and kapillary 7 is provided with cone-shaped cavity 5 places of connection can stop the platform 15 that kapillary 7 samples are drawn automatically;
Said locking system 3 is the draw and insert-type rhabodoids that are interspersed between cavity 4 outer walls and the pusher arm 1, only needs when removing locking its extraction and pusher arm 1 disengaging are got final product;
Said locking system 3 is the rhabodoids that are fixed between cavity 4 outer walls and the pusher arm 1, and it inserts cavity 4 parts and can under the following acting force of pusher arm 1, fracture, and removes its locking to pusher arm 1;
Packed-piston that is used for store reagents and the set diversion trench of its underpart cavity inner wall in the said cavity are used; The sequencing of said different reagent and sample mixing is regulated and control to the spacing between the lower guide chute through packed-piston, thereby realizes the purpose of mixing of different reagent and sample;
The quantity of said diversion trench, the degree of depth and width can be provided with 1-6 with the need;
In order to realize more kinds of reagent and sample mixing; Said cavity 4 can be combined by a plurality of single cavity of the shared connection cone-shaped cavity 5 in bottom; Each single cavity has the independently packed-piston 2 of top band pusher arm 1 respectively, can need set the packed-piston 9 of varying number and the diversion trench 12 that matches in its underpart with reagent type in single cavity;
In order to realize reagent and multiple sample mixing; Said cavity 4 can be communicated with a plurality of single cavity that has kapillary 7 separately by the bottom and combine; Each single cavity has the packed-piston 2 that top independently has pusher arm 1 respectively, can need set the packed-piston 9 of varying number and the diversion trench 12 that matches in its underpart with reagent type in single cavity.
Liquid sample processing device of the present invention, rational in infrastructure, easy to operate, be applicable to multiple condition of different needs, can save operation steps numerous and diverse in the prior art, make to detect to become simple and easy to do and can be applied to real-time test.
Four, description of drawings
Fig. 1: the structural representation of embodiments of the invention 1 liquid sample processing device;
The planar structure synoptic diagram of Fig. 1-A: Fig. 1; The axial section of Fig. 1-B: Fig. 1-A;
Fig. 1-D: the A-A sectional view among Fig. 1-B;
Fig. 2: the structural representation of embodiments of the invention 2 liquid sample processing devices;
The planar structure synoptic diagram of Fig. 2-A: Fig. 2; The axial section of Fig. 2-B: Fig. 2-A;
Fig. 3: the structural representation of embodiments of the invention 3 liquid sample processing devices;
The planar structure synoptic diagram of Fig. 3-A: Fig. 3; The axial section of Fig. 3-B: Fig. 3-A;
Fig. 3-D: B-B sectional view among Fig. 3-B; Fig. 3-E: C-C sectional view among Fig. 3-B;
Fig. 4: the structural representation of embodiments of the invention 4 liquid sample processing devices;
The planar structure synoptic diagram of Fig. 4-A: Fig. 4; The axial section of Fig. 4-B: Fig. 4-A;
Fig. 4-D: D-D sectional view among Fig. 4-B; Fig. 4-E: E-E sectional view among Fig. 4-B;
Fig. 5: the structural representation of embodiments of the invention 5 liquid sample processing devices;
The planar structure synoptic diagram of Fig. 5-A: Fig. 5; The axial section of Fig. 5-B: Fig. 5-A;
Fig. 5-D: F-F sectional view among Fig. 5-B;
Fig. 6: the structural representation of embodiments of the invention 6 liquid sample processing devices;
The planar structure synoptic diagram of Fig. 6-A: Fig. 6; The axial section of Fig. 6-B: Fig. 6-A;
Fig. 6-D: G-G sectional view among Fig. 6-B; Fig. 6-E: H-H sectional view among Fig. 6-B;
Five, embodiment
Following with reference to accompanying drawing, provide embodiment of the present invention, be used for formation of the present invention is further specified.
Embodiment 1
The liquid sample processing device of present embodiment is with reference to figure 1, Fig. 1-A, Fig. 1-B, Fig. 1-D; There is a kapillary 7 to be used to draw quantitative liquid sample; Kapillary 7 has platform 15 with cone-shaped cavity 5 places of connection, and platform 15 can stop the sample of kapillary 7 automatically and draw; A cavity 4 is connected with cone-shaped cavity 5, has a packed-piston 9 to be used for isolating reagent in the cavity 4 and makes reagent 8 be stored in cavity; The packed-piston 2 that links to each other with pusher arm 1 can move axially along cavity, and storing between reagent 8 and the packed-piston 2 has air; Locking system 3 fixedly pusher arm 1 makes packed-piston 2 and packed-piston 9 be in lockup state, locking system 3 can be the rhabodoid that when using pusher arm 1, takes out also can be formula easy to break apply certain pressure in pusher arm 1 time can fracture; Four diversion trenchs 11,12,13 and 14 are arranged on the wall of cavity 4.During use; Kapillary 7 is contacted with liquid sample, can be full of kapillary 7 automatically, take out or backfin locking system 3 through the capillarity sample; Bring pressure to bear on pusher arm 1; The pressure that packed-piston 2 and packed-piston 9 move down generation injects reaction cup with the sample in the kapillary 7, can stop when packed-piston 9 moves to the diversion trench position, and reagent 8 gets into cone-shaped cavity 5 and injects reaction cup and sample mixing through kapillary 7 along diversion trench; Continue to promote pusher arm 1; Make packed-piston 2 continue to move down, until reagent 8 and air are injected reaction cup fully, can be at the reagent and the pressure of the process of air injection reaction cup fully with reagent and sample mixing.
Embodiment 2
The liquid sample processing device of present embodiment has a kapillary 7 to be used to draw quantitative liquid sample with reference to figure 2, Fig. 2-A, Fig. 2-B, and kapillary 7 has platform 15 with cone-shaped cavity 5 places of connection, and platform 15 can stop the sample of kapillary 7 automatically and draw; A cavity 4, the bottom of cavity 4 have a diaphragm seal 18 to be used for isolating reagent to make reagent 8 be stored in cavity; Cone-shaped cavity 5 is tightly connected through screw thread 17 with cavity 4; The packed-piston 2 that links to each other with pusher arm 1 can move axially along cavity, and storing between reagent 8 and the packed-piston 2 has air; Puncturing lever 16 is used for puncture through seal film 18; Locking system 3 fixedly pusher arm 1 makes packed-piston 2 be in lockup state, locking system 3 can be the rhabodoid that when using pusher arm 1, takes out also can be formula easy to break apply certain pressure in pusher arm 1 time can fracture.During use, kapillary 7 is contacted with liquid sample, can be full of kapillary 7 automatically through the capillarity sample; Promote puncturing lever puncture through seal film 18, cavity 4 can be communicated with cone-shaped cavity 5, takes out or backfin locking system 3; Bring pressure to bear on pusher arm 1; The pressure that packed-piston 2 moves down generation injects the reaction cup mixing with sample in the kapillary 7 and reagent 8, continues to promote pusher arm 1, makes packed-piston 2 continue to move down; Until reagent 8 and air are injected reaction cup fully, can be at the reagent and the pressure of the process of air injection reaction cup fully with reagent and sample mixing.
Embodiment 3
The liquid sample processing device of present embodiment is with reference to figure 3, Fig. 3-A, Fig. 3-B, Fig. 3-D, Fig. 3-E, and the key distinction of present embodiment and embodiment 1 is to store two kinds of reagent in the cavity 4.Have a kapillary 7 to be used to draw quantitative liquid sample, kapillary 7 has platform 15 with cone-shaped cavity 5 places of connection, and platform 15 can stop the sample of kapillary 7 automatically and draw; A cavity 4; Be connected with cone-shaped cavity 5; Packed-piston 9-1 is used to isolate reagent liquid 8-1 and reagent 8-2 in the cavity 4, and reagent 8-2 can be that liquid reagent also can be a powdered reagent, as 8-2 be powdered reagent then should be designed so that reagent 8-1 earlier and after 8-2 mixes again with sample mixing; Packed-piston 9-2 is used for isolating reagent 8-2 and is stored in cavity 4 in the cavity 4; The packed-piston 2 that links to each other with pusher arm 1 can move axially along cavity, and all storing between reagent 8-2 and the packed-piston 9-1 and between reagent 8-1 and the packed-piston 2 has air; Locking system 3 fixedly pusher arm 1 makes packed-piston 2 and packed-piston 9 be in lockup state, locking system 3 can be the rhabodoid that when using pusher arm 1, takes out also can be formula easy to break apply certain pressure in pusher arm 1 time can fracture; Two groups of diversion trenchs are up and down arranged on the wall of cavity 4, and the quantity of every group of diversion trench is more than one, and 3 diversion trench 12-1,11-1,13-1 are used in present embodiment B-B cross section, and 2 diversion trench 12-2,14-2 are used in the C-C cross section; Gap through adjustment packed-piston 9-1 and diversion trench can set the sequencing of two kinds of reagent and sample mixing apart from the gap of 10-1 and packed-piston 9-2 and diversion trench apart from 10-2; After then reagent 8-1 and reagent 8-2 mix earlier less than 10-2 like 10-1 again with sample mixing, as 10-1 greater than 10-2 then reagent 8-2 first with sample mixing after mix with reagent 8-1 again; During use; Kapillary 7 is contacted with liquid sample; Can be full of kapillary 7 automatically through the capillarity sample, take out or backfin locking system 3, bring pressure to bear on pusher arm 1; The pressure that packed-piston 2 and packed-piston 9-1 and 9-2 move down generation injects reaction cup with sample in the kapillary 7 and the reagent in the cavity, and according to big young pathbreaker's reagent 8-1 and reagent 8-2 priority and the sample mixing mixing of 10-1 and 10-2.
Embodiment 4
The liquid sample processing device of present embodiment is with reference to figure 4, Fig. 4-A, Fig. 4-B, Fig. 4-D, Fig. 4-E; Present embodiment can be regarded the set of embodiment 1 and the device of embodiment 3 as, and cavity 4 and two pusher arm 1-1, the 1-2 of a kapillary 7 and two store reagents arranged.Have a kapillary 7 to be used to draw quantitative liquid sample, kapillary 7 has platform 15 with cone-shaped cavity 5 places of connection, and platform 15 can stop the sample of kapillary 7 automatically and draw; Two cavity 4-1 and 4-2 are connected with cone-shaped cavity 5, and two pusher arm 1-1 and 1-2 have a packed-piston 9-3 to be used for isolating reagent and make reagent 8-3 be stored in cavity among the cavity 4-1; The packed-piston 2-1 that links to each other with pusher arm 1-1 can move axially along cavity, and storing between reagent 8-3 and the packed-piston 2-1 has air; Packed-piston 9-1 is used to isolate reagent 8-1 and reagent 8-2 among the cavity 4-2, and packed-piston 9-2 is used for isolating reagent 8-2 and is stored in cavity 4-2 among the cavity 4-2; The packed-piston 2-2 that links to each other with pusher arm 1-2 can move axially along cavity, and all storing between reagent 8-2 and the packed-piston 9-1 and between reagent 8-1 and the packed-piston 2-2 has air; Locking system 3-1 is pusher arm 1-1 fixedly, and locking system 3-2 is pusher arm 1-2 fixedly.The packed-piston 9-2 cavity inner wall of packed-piston 9-1 and bottom is provided with diversion trench 11-1,12-1,13-1,14-1 among the cavity 4-2; Be respectively equipped with diversion trench 11-3,12-3,13-3,14-3 and diversion trench 11-2,12-2,13-2,14-2 among the cavity 4-1 among packed-piston 9-3 and the cavity 4-2 on the lower cavity inwall of packed-piston 9-2, the gap through adjusting each packed-piston and corresponding diversion trench can set the sequencing of each reagent and sample mixing apart from 10-1,10-2 and 10-3.
Embodiment 5
The liquid sample processing device of present embodiment is with reference to figure 5, Fig. 5-A, Fig. 5-B, Fig. 5-D; Present embodiment can be regarded the set of the device of two embodiment 3 as, and cavity 4-1,4-2 and two pusher arm 1-1,1-2 that have packed-piston 2-1,2-2 of two kapillary 7-1,7-2 and two store reagents are arranged.Kapillary 7-1,7-2 have platform 15-1,15-2 with the lower cavity place of connection, and platform 15-1,15-2 can stop the sample of kapillary 7-1,7-2 automatically and draw; Be respectively equipped with locking system 3-1,3-2 between pusher arm 1-1,1-2 and cavity 4-1, the 4-2, kapillary 7-1 is communicated with cavity 4-1, and kapillary 7-2 is connected with cavity 4-2; Store two kinds of reagent 8-4,8-5 by packed-piston 9-4,9-5 in the cavity 4-1; Each correspondence of bottom of packed-piston 9-4,9-5 is provided with diversion trench 11-4,12-4,13-4 and diversion trench 11-5,12-5,13-5 in the cavity 4-1; Spacing between packed-piston 9-4,9-5 and the lower guide chute is respectively 10-4,10-5; Store three kinds of reagent 8-1,8-2,8-3 by packed-piston 9-1,9-2,9-3 in the cavity 4-2; Packed-piston 9-1,9-2,9-3 bottom correspondence are provided with diversion trench 11-1,12-1,13-1 in the cavity 4-2; Diversion trench 11-2,12-2,13-2 and diversion trench 11-3,12-3,13-3, the spacing between packed-piston 9-1,9-2,9-3 and the lower guide chute is 10-1,10-2,10-3, this device can be handled two liquid samples simultaneously.
Embodiment 6
The liquid sample processing device of present embodiment is with reference to figure 6, Fig. 6-A, Fig. 6-B, Fig. 6-D, Fig. 6-E, and present embodiment can be regarded the set of the device of two embodiment 4 as, and cavity and four pusher arms of two kapillaries and four store reagents are arranged.Each kapillary is connected with 2 cavitys, be used for handling simultaneously two liquid samples, and each sample needs plurality of reagents to handle.

Claims (8)

1. liquid sample processing device; The characteristic part is to comprise that at least one internal storage has first cavity (4) of at least a reagent; First cavity (4) bottom is provided with at least one and is communicated with kapillary (7) with it; Be provided with the packed-piston (2) of at least one top band pusher arm (1) in each first cavity (4); The bottom correspondence of the packed-piston (2) of each top band pusher arm be provided with at least one be used to isolate reagent, with cavity supporting belt water conservancy diversion channel, can be through moving with the obturator of sample and reagent mix; Reagent is positioned between packed-piston (2) and the obturator, and mixing through water conservancy diversion channel set in first cavity (4) between the interior reagent of first cavity (4) and the interior sample of kapillary (7) realizes;
Said liquid sample processing device is characterized in that said first cavity (4) bottom is communicated with kapillary (7) through the bottom of taper second cavity (5) that is attached thereto;
Be provided with between said pusher arm (1) and first cavity (4) outer wall and be used to make packed-piston (2) to be in the locking system (3) of lockup state; The bottom of taper second cavity (5) has the kapillary (7) that is used to draw the quantitative liquid sample, and kapillary (7) is provided with taper second cavity (5) place of connection can stop the platform (15) that kapillary (7) sample is drawn automatically.
2. according to the said liquid sample processing device of claim 1, it is characterized in that
It is liquid reagent that the said reagent (8) that is stored in first cavity (4) has at least a kind of, and it is stored between packed-piston (2) and the obturator, and storing between reagent and the packed-piston has air or other kinds gas.
3. according to claim 1 or 2 said liquid sample processing devices, it is characterized in that
Said obturator for that be used with the water conservancy diversion channel, that fit tightly with first cavity (4) inwall, be used to isolate reagent (8) and make it sealing and be stored in the packed-piston (9) in first cavity (4), the water conservancy diversion channel is set at least 1 diversion trench (12) of first cavity (4) lower cavity inwall.
4. according to claim 1 or 2 said liquid sample processing devices, it is characterized in that
Said obturator is the diaphragm seal (18) in first cavity (4) bottom seals being used for of connecting reagent (8) sealing being stored in, the puncturing lever (16) of water conservancy diversion channel for being inserted into the inner by first cavity (4) top.
5. according to the said liquid sample processing device of claim 1, it is characterized in that said locking system (3) is the draw and insert-type rhabodoid that is interspersed between first cavity (4) outer wall and the pusher arm (1).
6. according to the said liquid sample processing device of claim 1, it is characterized in that said locking system (3) be fixed between first cavity (4) outer wall and the pusher arm (1), it inserts the rhabodoid that first cavity (4) part fractures under the following acting force of pusher arm (1).
7. according to claim 1 or 2 said liquid sample processing devices; It is characterized in that said first cavity (4) is combined by a plurality of single cavity of shared connection taper second cavity in bottom (5), each single cavity has the independently packed-piston (2) of top band pusher arm (1) respectively.
8. according to claim 1 or 2 said liquid sample processing devices; It is characterized in that said first cavity (4) is communicated with separately a plurality of single cavity that has kapillary (7) by the bottom and combines, each single cavity has the packed-piston (2) that top independently has pusher arm (1) respectively.
CN2009100163258A 2009-06-05 2009-06-05 Liquid sample processing device Expired - Fee Related CN101581729B (en)

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CN101581729B true CN101581729B (en) 2012-06-27

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CN102072949B (en) * 2010-11-11 2014-01-29 石家庄禾柏生物技术股份有限公司 Integrated quantitative sampling and reagent adding device
CN103776660B (en) 2012-10-24 2016-03-09 艾博生物医药(杭州)有限公司 A kind of device
ES2718069T3 (en) * 2014-04-30 2019-06-27 Merck Patent Gmbh Device for sample preparation and method for preparing a sample for sterility tests
CN107576809B (en) * 2017-09-30 2024-07-23 江苏宝卫士生物科技有限公司 Instant inspection device
CN107817358B (en) * 2017-12-14 2023-11-07 江苏宝卫士生物科技有限公司 Diagnostic reagent detection device and method thereof
CN107866196B (en) * 2017-12-14 2023-09-12 江苏宝卫士生物科技有限公司 Conjoined feeding device and method thereof
CN109738662A (en) * 2019-03-07 2019-05-10 深圳奥萨制药有限公司 A kind of POCT Biochemical Analyzer with multi-channel detection
CN114618419A (en) * 2020-12-11 2022-06-14 深圳市帝迈生物技术有限公司 Sample filling method, sample filling assembly and sample analyzer

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CN88102077A (en) * 1987-04-14 1988-10-26 阿斯特拉医疗技术公司 Automatic Two-chamber Syringe
CN1054374A (en) * 1990-01-22 1991-09-11 诺沃挪第克公司 A kind of method and apparatus that is used to mix with injectable drug
CN2860495Y (en) * 2005-12-31 2007-01-24 湖南千山制药机械股份有限公司 Pre-filled injector with needle for mixed drug injection
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