Background technology:
Along with modern civilization construction and the commercial continuous development that needs, the improving constantly of people's daily life level improves constantly the requirement of bath article.What people used when washing is taken a shower traditionally is with the soap series products of soap as main component.The pH value meta-alkalescence of this series products, inharmonious with the acid ph value of human body skin, thereby zest is bigger, and also the fatty acid resistance to hard water is poor, can form calcium soap, magnesium soap when taking a shower, and is deposited on the skin, hinders the breathing and the metabolism of skin.Along with development of science and technology, bath gel has appearred on the market, can remove the dirt of skin effectively, can not form calcium soap, magnesium soap simultaneously, be deposited on the skin.
In the process of using bath gel, people not only requirement can remove dirt, also wish to kill the pathogenic microorganism on the health.
On skin, people's perspiration has formed dirt with scurf of dust in air and human body etc.Assembled a large amount of microorganisms on dirt, some are wherein just arranged may be the source of disease microorganism, and these health to people are brought certain threat.Especially some outdoor labourers, because the restriction of sanitary condition, dirt is more on the skin, has assembled a large amount of microorganisms above, wherein just having some is morbific microorganisms.In the more environment of source of disease germs collect such as hospital, the source of disease microorganism also can accumulate on the skin.Temperature relatively is fit to the breeding of microorganism on the skin, and the source of disease microorganism may obtain a large amount of breedings.The dirt that can remove skin of bath gel can be removed a certain amount of source of disease microorganism simultaneously, but our common bath gel does not possess sterilizing function, so can't thoroughly remove and pathogenic microbe killing.Therefore may bring threat to our health.Bath gel in the market seldom has sterilizing function.
In the bath gel, generally add antiseptic and prevent the putrid and deteriorated of bath gel at present, these antiseptic have certain danger to human beings'health, may certain stimulation be arranged to skin simultaneously.That adds among the present invention has the antiseptical function by the pure natural fermentation raw material through the antibacterial that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again antiseptic in the bath gel.
Summary of the invention:
The purpose of this invention is to provide a kind of highly effective and safe, adopt the sterilization shower cream product of modern biotechnology product processing.
Key component of the present invention comprises compositions such as anion surfactant, non-ionic surface active agent, amphoteric surfactant, antibacterial, thickening agent, nutrient, emollient, glycerol, essence, deionized water.Antibacterial is for being the fermentation broth extract of bacillus subtilis CGMCC 2294.
Major technique feature of the present invention be added have inexpensive, highly effective and safe, all-natural is through the antibacterial of modern biotechnology processing.The used antibacterial of the present invention is the inventor's an invention achievement.Antibacterial is bacillus subtilis LD-8 (Bacillus subtilis LD-8) fermentation broth extract, culture presevation is in China Microbial Culture Preservation Commission common micro-organisms center, Datun Road, Chaoyang District, Beijing City, preservation address, preservation date December in 2007 18 days, culture presevation numbering CGMCC 2294.
Fungus characteristic is as follows:
1. somatic cells is life or the living Gram-positive of near-end in direct rod shape, the spore.
2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.
3. can utilize various saccharides, as glucose, galactose, xylose, cellobiose, cottonseed sugar etc.; Produce amylase, protease, can gelatin hydrolysate, V.P, the C.I. 13020. positive.
4. 37 ℃ of the suitableeest cultivation temperature, optimum pH 7.0.
This bacterium classification status is as follows: antibacterial territory (Bacteria); Firmicutes (Firmicutes); Bacillus cereus guiding principle (Bacillales); Bacillus cereus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis strain (Bacillus subtilis).
The preparation method of fermentation broth extract is as follows among the present invention:
The seed culture medium of bacterial strain is a broth bouillon, Carnis Bovis seu Bubali cream 3g wherein, peptone 10g, NaCl5g, water 1000ml, pH value 7.0-7.2, high pressure steam sterilization.
The seed liquor condition of culture: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 10-14 hour.
When inoculum concentration is 6%-10%, help the formation of antibacterial substance.
The fermentation medium of optimizing consists of: sucrose 0.3%, and peptone 1%, NaCl 0.5%, MgSO
47H
2O 0.7%,
KH
2PO
4?0.7%,pH8.0。
The fermentation culture conditions of optimizing is: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.
Extracting the antibacterial substance method from fermentation liquid is organic solvent precipitation method.Available organic solvent be acetone, methanol,
Ethanol.
At first with fermentation liquid, add high speed centrifuge, temperature 0-4 ℃, rotating speed is 10000 rev/mins, centrifugal ten minutes, remove precipitation, and keep supernatant.
With the supernatant that obtains, add isopyknic methanol, the temperature of system is reduced to-5 ℃ rapidly, preserved 2 hours for-5 ℃.
Separate obtaining organic facies, adopt vacuum distillation method to remove organic solvent.Vapo(u)rizing temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed.The collecting precipitation material.This promptly is the crude extract of our Fungicidal substance that obtains.
The crude extract of Fungicidal substance, behind water dissolution, concentration is 1% (mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can kill escherichia coli effectively, Salmonella, the pathogenic microorganism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.
This bactericidal liquid has high safety, in the emergency toxicology experiment, we gavage 5 groups of 200 mouse with this bactericidal liquid, and the mouse of experimental group none occur dead, and bacillus subtilis is present in occurring in nature widely, and many important industrial bacterial strains are exactly bacillus subtilis.Substantially can conclude that this bactericidal liquid is safe for user, can relievedly use.
Bath gel method for making of the present invention is simple, adding the part deionized water goes in the reactor, its temperature is heated to 60-70 ℃ adds man-hour anion surfactant 1-10%, non-ionic surface active agent 1-5%, amphoteric surfactant 1-5%, glycerol 3-6%, antilubricant 1-10%, emollient 0.5-3% and deionized water and stirring are even, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5-7.0, add nutrient 0.5-3%, antibacterial 0.01-1%, essence 0.0001-0.0004%, thickening agent 1-4%, insufficient section deionized water polishing, stir, speed of agitator can be controlled in 20-30 rev/min, and the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that forms in the stirring and dissolving process, reduces the air content of material, mixed liquor is carried out packing, can obtain high-efficiency sterilization shower cream.Ratio is the percentage by weight of each ingredients constitute product gross weight herein.
Anion surfactant is a sodium lauryl sulphate, and is at least a in the Sodium Alcohol Ether Sulphate.
Non-ionic surface active agent can be an alkylphenol polyoxyethylene, and is at least a in the fatty alcohol-polyoxyethylene ether.
Amphoteric surfactant is a dodecyldimethylammonium hydroxide inner salt, and is at least a in the dodecayl dimethyl amine oxide.
Thickening agent is a polyethylene glycol mono stearate
Glycerol can prevent that water content reduces in the skin, and can improve the tolerance to cold of product as wetting agent, the variation that can not produce form when product is used.
Nutrient comprises one or more in vitamin or the collagen protein.
Emollient comprises in water-soluble modified silicone oil, cation-modified silicone oil or the amino-modified silicone oil at least a.
Used essence can add according to the odor type of product, makes product have different odor types, satisfies the needs of different consumers.
Beneficial effect:
The present invention has not only realized the function of bath gel removal dirt, and has possessed good sterilization and disinfectant function, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the antibacterial that adds in this detergent people and animals to be had very high safety.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat for people's health.The production of the antibacterial that adds in the bath gel utilizes fermentation raw material with low cost, adopts modern biotechnology, and technology is simple, and is with low cost.Make the present invention, it is strong to become a kind of sterilizing power, safety, cheapness, bath gel with high content of technology.
That adds among the present invention has the antiseptical function by the pure natural fermentation raw material through the antibacterial that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again antiseptic in the bath gel.
Specific embodiment:
The invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.
Embodiment 1
Prepare 100 kilograms of products, 60 kilograms of deionized waters are added in the reactor, its temperature is heated to 70 ℃, add the 4.5kg Sodium Alcohol Ether Sulphate then successively, 3.2kg fatty alcohol-polyoxyethylene ether, the 2kg dodecyldimethylammonium hydroxide inner salt, 3kg glycerol, 4kg antilubricant, adding the water-soluble modified silicone oil of 0.5kg then stirs, speed of agitator is unsuitable too high, can be controlled in 20-30 rev/min, after stirring, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5, add the 1kg collagen protein, the 0.2kg antibacterial, rose essence 0.0001%, 2kg polyethylene glycol mono stearate, insufficient section add the deionized water polishing, stir, speed of agitator can be controlled in 20-30 rev/min, the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out packing, can obtain high-efficiency sterilization shower cream.
This product adopts conventional microorganism detection bactericidal effect with the Safeguard product, and result of the test shows that product of the present invention under equal conditions bactericidal effect improves 60-80% than Safeguard, and bactericidal effect is obvious.Escherichia coli, Salmonella, golden yellow common coccus are selected in microorganism.
Embodiment 2
Prepare 100 kilograms of products, 70 kilograms of deionized waters are added in the reactor, its temperature is heated to 60 ℃, add the 6kg sodium lauryl sulphate then successively, the 4kg alkylphenol polyoxyethylene, the 4kg dodecayl dimethyl amine oxide, 5kg glycerol, 6kg antilubricant, adding the water-soluble modified silicone oil of 1kg then stirs, speed of agitator is unsuitable too high, can be controlled in 20-30 rev/min, after stirring, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 7, add the 0.5kg collagen protein, the 0.01kg antibacterial, rose essence 0.0002%, 1kg polyethylene glycol mono stearate, insufficient section add the deionized water polishing, stir, speed of agitator can be controlled in 20-30 rev/min, the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out packing, can obtain high-efficiency sterilization shower cream.