CN101507702B - Sterilizing cleansing facial milk - Google Patents
Sterilizing cleansing facial milk Download PDFInfo
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- CN101507702B CN101507702B CN2008101017678A CN200810101767A CN101507702B CN 101507702 B CN101507702 B CN 101507702B CN 2008101017678 A CN2008101017678 A CN 2008101017678A CN 200810101767 A CN200810101767 A CN 200810101767A CN 101507702 B CN101507702 B CN 101507702B
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Abstract
The invention discloses cleansing cream with high-efficiency sterilization, and belongs to the field of hygiene products. The invention aims to provide a bactericidal cleansing cream product which is high-efficient and safe and is processed by adopting modern biotechnology. A formula of the cleansing cream mainly comprises a surfactant, a humectant, a nutritional agent, an emollient, essence, anda bactericide. The invention realizes that the cleansing cream removes dirt on skin, and also has good sterilization and disinfection functions. The cleansing cream utilizes a fermentation raw material with low cost, adopts the modern biotechnology, and has simple process and low cost. Thus the invention becomes the cleansing cream with strong sterilizing power, safety, low cost, and high technology content. The bactericide which is added into the cleansing cream and is obtained by processing the pure natural fermentation raw material through the modern biotechnology has the function of antisepsis and the characteristics of safety and high efficiency. No preservative needs to be added into the cleansing cream.
Description
Technical field: the invention belongs to field of hygiene products, relate to special cleansing milk.
Background technology:
Cleansing milk has become a lot of people, particularly indispensable article of everyday use in the youngster daily life.
In actual life, along with improving constantly of people's material and cultural life, U.S.A of natural health becomes the target that people pursue.Various skin care item arise at the historic moment on the market.What people used when washing one's face traditionally is with the soap series products of soap as main component.The pH value meta-alkalescence of this series products, inharmonious with the acid ph value of human body skin, thereby zest is bigger, and also the fatty acid resistance to hard water is poor, can form calcium soap, magnesium soap when taking a shower, and is deposited on the skin, hinders the breathing and the metabolism of skin.In order to remedy the deficiency of soap, cleansing milk arises at the historic moment.
Cleansing milk is exactly to adopt the mode of washing to clean the emulsion form product of human body face.The basis of cleansing milk at present commonly used is water and surfactant.Add additives such as essence, wetting agent, antiseptic, antioxidant with stable prod, give its fragrance.
People's perspiration has formed dirt with scurf of dust in air and human body etc.Assembled a large amount of microorganisms on dirt, some are wherein just arranged may be the source of disease microorganism, and these health to people are brought certain threat.Yet,, can not kill the antibacterial of people's face effectively though the most cleansing milk in market can play good cleaning effect.Facial antibacterial can cause people to form acne, also might cause some diseases simultaneously.In the cleansing milk, generally add antiseptic and prevent the putrid and deteriorated of bath gel at present, these antiseptic have certain danger to human beings'health, simultaneously skin are had certain stimulation.
Summary of the invention:
The purpose of this invention is to provide a kind of highly effective and safe, adopt the sterilization cleansing milk product of modern biotechnology product processing.
Prescription of the present invention mainly contains: surfactant, wetting agent, nutrient, emollient, essence, antibacterial.
Major technique feature of the present invention be added have inexpensive, highly effective and safe, all-natural is through the antibacterial of modern biotechnology processing.The used antibacterial of the present invention is the inventor's an invention achievement.Antibacterial is bacillus subtilis LD-8 (Bacillus subtilis LD-8) fermentation broth extract, and culture presevation is in China Microbial Culture Preservation Commission common micro-organisms center, and CGMCC 2294 is numbered in culture presevation).
Fungus characteristic is as follows:
1. somatic cells is life or the living Gram-positive of near-end in direct rod shape, the spore.
2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.
3. can utilize various saccharides, as glucose, galactose, xylose, cellobiose, cottonseed sugar etc.; Produce amylase, protease, can gelatin hydrolysate, V.P, the C.I. 13020. positive.
4. 37 ℃ of the suitableeest cultivation temperature, optimum pH 7.0.
This bacterium classification status is as follows: antibacterial territory (Bacteria); Firmicutes (Firmicutes); Bacillus cereus guiding principle (Bacillales); Bacillus cereus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis strain (Bacillus subtilis).
The preparation method of fermentation broth extract is as follows among the present invention:
The seed culture medium of bacterial strain is a broth bouillon, Carnis Bovis seu Bubali cream 3g wherein, peptone 10g, NaCl 5g, water 1000ml, pH value 7.0～7.2, high pressure steam sterilization.
The seed liquor condition of culture: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 10～14 hours.
Inoculum concentration is 6%～10% o'clock, helps the formation of antibacterial substance.
The fermentation medium of optimizing consists of: sucrose 0.3%, and peptone 1%, NaCl 0.5%, and MgSO4.7H2O 0.7%, and KH2PO4 0.7%, pH8.0.
The fermentation culture conditions of optimizing is: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.
Extracting the antibacterial substance method from fermentation liquid is organic solvent precipitation method.Available organic solvent is acetone, methanol, ethanol.
At first with fermentation liquid, add high speed centrifuge, temperature 0-4 ℃, rotating speed is 10000 rev/mins, centrifugal ten minutes, remove precipitation, and keep supernatant.
With the supernatant that obtains, add isopyknic methanol, the temperature of system is reduced to-5 ℃ rapidly, preserved 2 hours for-5 ℃.Collect organic facies, adopt vacuum distillation method to remove organic solvent.Vapo(u)rizing temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed.The collecting precipitation material.This promptly is the crude extract of our Fungicidal substance that obtains.
The crude extract of Fungicidal substance, behind water dissolution, concentration is 1% (mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can kill escherichia coli effectively, Salmonella, the pathogenic microorganism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.
This bactericidal liquid has high safety, in the emergency toxicology experiment, we gavage 5 groups of 200 mouse with this bactericidal liquid, and the mouse of experimental group none occur dead, and bacillus subtilis is present in occurring in nature widely, and many important industrial bacterial strains are exactly bacillus subtilis.Substantially can conclude that this bactericidal liquid is safe for user, can relievedly use.After adding it to cleansing milk, find that it still has good bactericidal activity.
Method for making of the present invention is simple, with deionized water, surfactant (5～20%), wetting agent (3～6%), nutrient (0.5～3%), emollient (0.5～3%), antibacterial (0.1～1%) and essence, join in the reactor, stir, regulate acid-base value, can obtain cleansing cream with high-efficiency sterilization through the degassing, fill.The adding proportion of each material is the percentage ratio of gross weight.
Concrete technology is as follows: deionized water is added in the reactor, its temperature is heated to 70 ℃, the cocamido propyl betaine that adds the regulation ratio then successively, trialkyl methyl oxidation amine, coconut monoethanolamide, glycerol, water-soluble modified silicone oil stirs, and speed of agitator is unsuitable too high, can be controlled in 20～30 rev/mins, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5-7.0, add collagen protein, antibacterial, essence, insufficient section stirs with the deionized water polishing, and speed of agitator can be controlled in 20～30 rev/mins, and the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that forms in the stirring and dissolving process, reduces the air content of material, mixed liquor is carried out packing, can obtain efficient sterilizing and wash milk.
Surfactant, this product preferred surfactants has fatty alcohol-polyoxyethylene ether, Oleum Cocois single ethanol amide, cocamido propyl betaine, one or more in cocamidopropyl propyl amide amine oxide, the trialkyl methyl oxidation amine etc.
Glycerol can prevent that water content reduces in the skin, and can improve the tolerance to cold of product as wetting agent, the variation that can not produce form when product is used.
Nutrient is a collagen protein.
Emollient comprises water-soluble modified silicone oil, cation-modified silicone oil, one or more of amino-modified silicone oil.
Citric acid is an amount of, is used to regulate pH value.
Used essence can add according to the odor type of product, makes product have different odor types, satisfies the needs of different consumers.
Beneficial effect:
The present invention has not only realized the dirt on the cleansing milk removal skin,, and possessed good sterilization and disinfectant function, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the antibacterial that adds in this cleansing milk people and animals to be had very high safety.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat for people's health.The production of the antibacterial that adds in the cleansing milk utilizes fermentation raw material with low cost, adopts modern biotechnology, and technology is simple, and is with low cost.Make the present invention, it is strong to become a kind of sterilizing power, safety, cheapness, cleansing milk with high content of technology.
That adds among the present invention has the antiseptical function by the pure natural fermentation raw material through the antibacterial that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again antiseptic in the cleansing milk.
Specific embodiment:
The invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.
Specific embodiment:
The invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.
Embodiment 1, preparation 100kg product.
The 50kg deionized water is added in the reactor, its temperature is heated to 70 ℃, add the 5kg cocamido propyl betaine then successively, 2kg trialkyl methyl oxidation amine, 2kg coconut monoethanolamide, 4kg glycerol, add the water-soluble modified silicone oil of 3kg then and stir, speed of agitator is unsuitable too high, can be controlled in 25 rev/mins, cool the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5, collagen protein 1kg adds the 0.2kg antibacterial, 0.001kg essence, insufficient section stirs with the deionized water polishing, and speed of agitator can be controlled in 25 rev/mins, and the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that forms in the stirring and dissolving process, reduces the air content of material, mixed liquor is carried out packing, can obtain efficient sterilizing and wash milk.
Get 10% concentration product of the present invention, adopt conventional microorganism detection bactericidal effect, result of the test shows that product bactericidal effect of the present invention reaches more than 90%, and bactericidal effect is obvious.Escherichia coli, Salmonella, golden yellow common coccus are selected in microorganism.
Claims (1)
1. a sterilization cleansing milk is characterized in that containing antibacterial, and described antibacterial is a bacillus subtilis CGMCC2294 fermentation broth extract; Described 100kg sterilization cleansing milk product is prepared by following method:
The 50kg deionized water is added in the reactor, its temperature is heated to 70 ℃, add the 5kg cocamido propyl betaine then successively, 2kg trialkyl methyl oxidation amine, 2kg coconut monoethanolamide, 4kg glycerol, adding the water-soluble modified silicone oil of 3kg then stirs, speed of agitator is controlled at 25 rev/mins, cools the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5, collagen protein 1kg, add the 0.2kg antibacterial, 0.001kg essence, insufficient section stirs with the deionized water polishing, speed of agitator can be controlled in 25 rev/mins, the feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure is controlled at 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out packing, can obtain the cleansing milk that sterilizes;
Bacillus subtilis CGMCC2294 fermentation broth extract preparation method is as follows:
The seed culture medium of bacterial strain is a broth bouillon, Carnis Bovis seu Bubali cream 3g wherein, peptone 10g, NaCl5g, water 1000ml, pH value 7.0～7.2, high pressure steam sterilization;
The seed liquor condition of culture: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 10～14 hours;
Fermentation medium consists of: sucrose 0.3%, and peptone 1%, NaCl 0.5%, MgSO
4.7H
2O 0.7%, KH
2PO
40.7%, pH8.0;
Fermentation culture conditions is: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 24 hours;
At first with fermentation liquid, add high speed centrifuge, temperature 0-4 ℃, rotating speed is 10000 rev/mins, centrifugal ten minutes, remove precipitation, and keep supernatant; With the supernatant that obtains, add isopyknic methanol, the temperature of system is reduced to-5 ℃ rapidly, preserved 2 hours for-5 ℃; Collect organic facies, adopt vacuum distillation method to remove organic solvent; Vapo(u)rizing temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed the collecting precipitation material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101017678A CN101507702B (en) | 2008-03-12 | 2008-03-12 | Sterilizing cleansing facial milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101017678A CN101507702B (en) | 2008-03-12 | 2008-03-12 | Sterilizing cleansing facial milk |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101507702A CN101507702A (en) | 2009-08-19 |
| CN101507702B true CN101507702B (en) | 2010-09-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101017678A Active CN101507702B (en) | 2008-03-12 | 2008-03-12 | Sterilizing cleansing facial milk |
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| Country | Link |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793654A (en) * | 2012-06-24 | 2012-11-28 | 邵素英 | Cleansing milk |
| CN104644490A (en) * | 2014-06-10 | 2015-05-27 | 莫治玲 | Facial cleaner |
| CN105943434B (en) * | 2016-05-18 | 2019-01-01 | 广州暨北生物科技有限公司 | A kind of facial cleanser and preparation method thereof for treating acne |
| CN111544371B (en) * | 2020-06-12 | 2020-11-20 | 洋浦吉商生物科技有限公司 | Whitening and repairing mask containing probiotic active extracting solution and nicotinamide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1432284A (en) * | 2002-01-18 | 2003-07-30 | 福建泰禾生化科技股份有限公司 | High-efficiency biological germicide and its prepn process |
| CN1853489A (en) * | 2005-04-28 | 2006-11-01 | 武汉绿世纪生物工程有限责任公司 | Antiseptic antistaling agent containing hay bacillocin |
| CN101041846A (en) * | 2007-01-29 | 2007-09-26 | 中国科学院等离子体物理研究所 | Preparation method and usage for novel lipopeptide type biosurfactant surfactin |
-
2008
- 2008-03-12 CN CN2008101017678A patent/CN101507702B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1432284A (en) * | 2002-01-18 | 2003-07-30 | 福建泰禾生化科技股份有限公司 | High-efficiency biological germicide and its prepn process |
| CN1853489A (en) * | 2005-04-28 | 2006-11-01 | 武汉绿世纪生物工程有限责任公司 | Antiseptic antistaling agent containing hay bacillocin |
| CN101041846A (en) * | 2007-01-29 | 2007-09-26 | 中国科学院等离子体物理研究所 | Preparation method and usage for novel lipopeptide type biosurfactant surfactin |
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| CN101507702A (en) | 2009-08-19 |
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Effective date of registration: 20200407 Address after: 053000 workshop of Hengshui Shangde Fuel Cell Technology Co., Ltd., Taocheng high tech Zone, Taocheng District, Hengshui City, Hebei Province Patentee after: Shengyi Hengshui Daily Chemical Co., Ltd Address before: 101121, 1 Cui Lu, Liyuan, Tongzhou District, Beijing, Zhenjiang Patentee before: BEIJING LVDAOYUAN BEE PRODUCT Co.,Ltd. |
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