CN101480135B - Device for cultivating liquid culture of edible fungus - Google Patents
Device for cultivating liquid culture of edible fungus Download PDFInfo
- Publication number
- CN101480135B CN101480135B CN2009100290677A CN200910029067A CN101480135B CN 101480135 B CN101480135 B CN 101480135B CN 2009100290677 A CN2009100290677 A CN 2009100290677A CN 200910029067 A CN200910029067 A CN 200910029067A CN 101480135 B CN101480135 B CN 101480135B
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- Prior art keywords
- pipe
- glass
- bulb
- double
- glass bulb
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 241000233866 Fungi Species 0.000 title claims abstract description 13
- 238000009630 liquid culture Methods 0.000 title 1
- 239000011521 glass Substances 0.000 claims abstract description 42
- 229920001971 elastomer Polymers 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 229920000742 Cotton Polymers 0.000 claims abstract description 4
- 229920001296 polysiloxane Polymers 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000000855 fermentation Methods 0.000 abstract description 3
- 230000004151 fermentation Effects 0.000 abstract description 3
- 238000009434 installation Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract 1
- 229920002379 silicone rubber Polymers 0.000 abstract 1
- 239000004945 silicone rubber Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 description 4
- 239000012531 culture fluid Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a culture apparatus for liquid strain in the edible fungi, which comprises a glass bottle (1), a rubber plug (2), a glass pipe (3), a double-bulb cavity glass bulb pipe (4) and a connecting hose (5). The culture apparatus is characterized in that the diameter of the big head of the rubber plug (2) is slightly more than that of the glass bottle cap, two holes are punched in the rubber plug (2), and the glass pipe (3) and the double-bulb cavity glass bulb pipe (4) are inserted in the holes; the glass pipe (3) is an air inlet pipe, the length of the glass pipe (3) is slightly higher than the height of the glass bottle, the double-bulb cavity glass bulb pipe (4) is connected to the upper end of the glass pipe (3) through the silicone rubber pipe (5), the lower end of the glass pipe (3) penetrates through the rubber plug, and the distance between the port and the bottle bottom is 1-2 cm; and the double-bulb cavity glass bulb pipe (4) is filled with clean cotton so as to be convenient for filtering the air. The invention can produce the liquid strain in the edible fungi instead of equipment such as fermentation tanks, oscillating table, and the like, has small investment and low production cost, and can save lots of electric power resources and running cost. In addition, the apparatus is made of material easy to obtain, is low in cost, rapid in installation and simple and convenient for operation, thereby being popularized and applied in wide rural households.
Description
Technical field
The present invention relates to the Edible Fungi field, particularly relate to a kind of strain cultivation device.
Background technology
Edible fungi liquid strain is with short production cycle, and speed is fast, and cell age is consistent, is fit to large-scale planting, is the developing direction of mushroom industry.Edible fungi liquid strain all uses fermentation tank or shaking table production at present, and equipment investment is big, and production cost is high, and a set of equipment needs several ten thousand yuan, and ordinary people family is difficult to bear, and has had a strong impact on edible fungi liquid strain technology applying in the rural area.
Summary of the invention
The object of the invention just provides a kind of with low cost, easy to operate, respond well; Ordinary people family can homemade simple and easy culture device for liquid strain of edible fungus; High to overcome existing equipment fermentation price, complicated operation is difficult for the drawback of applying in the rural area.
Technical scheme of the present invention realizes in the following manner: a kind of culture device for liquid strain of edible fungus, by culture vessel 1, rubber stopper 2, glass tube 3, two balls chamber glass tube 4 be connected flexible pipe 5 and form.It is characterized in that: the diameter at butt end of rubber stopper 2 is slightly larger than the culture vessel mouth, so that sealing.And on rubber stopper, make a call to 2 holes, be used to insert glass tube 3 and two ball chamber glass tube I4.Glass tube 3 is an air inlet pipe, and length is a little more than the height of vial, and the upper end connects two ball chamber glass bulb II4 with silicone tube 5, and rubber stopper is passed in the lower end, and port is from end 1-2 centimetre in bottle.Fill with clean cotton in the ball chamber of two balls chamber glass tube I and two ball chamber glass bulb II, so that filtered air.
Compare with the edible fungi liquid strain installation for fermenting of present use, the present invention can reach following effect: 1, practice thrift equipment investment, reduce production costs, simultaneously all right conservation of power resource and operating cost; 2, this device materials is easy to get, and is with low cost, quick for installation, easy and simple to handle, can be in rural households' wide popularization and application.
Description of drawings Fig. 1 is a structural representation of the present invention.1. vial, 2. rubber stopper, 3. glass tube, 4. pair ball chamber glass bulb 5. connects flexible pipe.
Embodiment: vials 1 such as the reagent bottle of purchase 0.5-10L, triangular flask, wide-mouth bottle; Select supporting rubber stopper 2 according to the bottleneck size; On rubber stopper 2, open 2 holes with card punch or electric drill, the aperture will be complementary with the external diameter of glass tube 3 and glass bulb 4.Glass tube 3 is inserted in the rubber stopper with two ball chamber glass bulb I4, and glass tube 3 is an air inlet pipe, and length is a little more than the height of vial, and the upper end connects two ball chamber glass bulb II4 with silica gel hose 5, and rubber stopper is passed in the lower end, and port is from end 1-2 centimetre in bottle.Two balls chamber glass bulb I4 fills with clean cotton with two ball chamber glass bulb II4 are interior, so that filtered air.Mounted air-breather is all wrapped up the position below the rubber stopper with double-deck newspaper or brown paper, and tie with rope, put into medical portable high-pressure sterilizing pot, 1.4 kg/cm pressure sterilizations 50 minutes are subsequent use.The edible fungi liquid strain culture fluid that configures is packed in the vial; The amount of packing into is the 1/2-2/3 of vial volume; Seal with polypropylene film then, the vial that culture fluid will be housed is again put into medical portable high-pressure sterilizing pot, and 1.1 kg/cm pressure sterilizations 35 minutes are subsequent use.At last, culture fluid and the rubber stopper of the bacterium of will going out are put into inoculating hood together, and access needs mother's kind of cultivation in aseptic environments, and the access amount is that the mother of soya bean size plants the 3-4 piece, and the jam-pack rubber stopper is put into culturing room and carries out aerobic culture.Need only during ventilation aseptic air is received on the two balls chamber glass bulb above the glass tube with flexible pipe.
Claims (1)
1. culture device for liquid strain of edible fungus; By vial (1), rubber stopper (2), glass tube (3), two balls chamber glass bulb (4) be connected flexible pipe (5) and form; It is characterized in that: the diameter at butt end of rubber stopper (2) is slightly larger than the glass bottle opening bore; Rubber stopper (2) is gone up and is made a call to 2 holes, inserts glass tube (3) and two ball chamber glass bulb I (4) respectively; Said glass tube (3) is an air inlet pipe, and length is a little more than the height of vial, and the upper end connects two ball chamber glass bulb II (4) with silicone tube (5), and rubber stopper is passed in the lower end, and port is from end 1-2 centimetre in bottle; Fill with clean cotton in said couple of ball chamber glass bulb I (4), the two balls chamber glass bulb II (4), so that filtered air.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100290677A CN101480135B (en) | 2009-01-18 | 2009-01-18 | Device for cultivating liquid culture of edible fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100290677A CN101480135B (en) | 2009-01-18 | 2009-01-18 | Device for cultivating liquid culture of edible fungus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101480135A CN101480135A (en) | 2009-07-15 |
| CN101480135B true CN101480135B (en) | 2012-04-11 |
Family
ID=40877555
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100290677A Expired - Fee Related CN101480135B (en) | 2009-01-18 | 2009-01-18 | Device for cultivating liquid culture of edible fungus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101480135B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102379209A (en) * | 2011-09-08 | 2012-03-21 | 镇江市食用菌研究所 | Bottle stopper |
| CN102388747A (en) * | 2011-09-08 | 2012-03-28 | 镇江市食用菌研究所 | Test tube plug |
| CN102676389B (en) * | 2012-01-04 | 2014-04-23 | 河南科技大学 | Edible fungi strain preservation method and device facilitating taking strains at any time |
| CN103834557A (en) * | 2012-11-26 | 2014-06-04 | 唐杰 | Manufacturing method of simple fermentation device for producing liquid strain |
| CN108300646B (en) * | 2018-03-27 | 2020-05-22 | 华南理工大学 | Uniform-speed sterile ventilation device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87210089U (en) * | 1987-07-13 | 1988-06-22 | 余兆丰 | Simple apparatus for culturing liquid strain in depth layer |
| CN1528127A (en) * | 2003-10-10 | 2004-09-15 | 中国科学院水生生物研究所 | Method for culturing nostoc using bioreactor |
-
2009
- 2009-01-18 CN CN2009100290677A patent/CN101480135B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87210089U (en) * | 1987-07-13 | 1988-06-22 | 余兆丰 | Simple apparatus for culturing liquid strain in depth layer |
| CN1528127A (en) * | 2003-10-10 | 2004-09-15 | 中国科学院水生生物研究所 | Method for culturing nostoc using bioreactor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101480135A (en) | 2009-07-15 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 212364 Danyang Jiangnan Town, Jiangsu City, the Yangtze River in Jiangsu Jiangnan Biotechnology Co., Ltd. Patentee after: Zhu Zhonggui Address before: 212008 Jiangsu city of Zhenjiang province Zongze Road No. 118 Zhenjiang Institute of edible fungi Patentee before: Zhu Zhonggui |
|
| ASS | Succession or assignment of patent right |
Owner name: JIANGSU XUNYUAN PLANTING INDUSTRY TECHNOLOGY CO., Free format text: FORMER OWNER: ZHU ZHONGGUI Effective date: 20140303 |
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| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 212364 ZHENJIANG, JIANGSU PROVINCE TO: 211302 NANJING, JIANGSU PROVINCE |
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| TR01 | Transfer of patent right |
Effective date of registration: 20140303 Address after: Gaochun District of Nanjing City, Jiangsu Province, 211302 Chatham town Jing Yang Village Patentee after: Jiangsu mushroom seed technology Co., Ltd. Address before: 212364 Danyang Jiangnan Town, Jiangsu City, the Yangtze River in Jiangsu Jiangnan Biotechnology Co., Ltd. Patentee before: Zhu Zhonggui |
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| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120411 Termination date: 20210118 |
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| CF01 | Termination of patent right due to non-payment of annual fee |