CN109794082A - It is a kind of for extracting the device of mononuclearcell in blood - Google Patents
It is a kind of for extracting the device of mononuclearcell in blood Download PDFInfo
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- CN109794082A CN109794082A CN201910214563.3A CN201910214563A CN109794082A CN 109794082 A CN109794082 A CN 109794082A CN 201910214563 A CN201910214563 A CN 201910214563A CN 109794082 A CN109794082 A CN 109794082A
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- area
- blood
- laydown
- mononuclearcell
- tube body
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Abstract
The embodiment of the invention discloses a kind of for extracting the device of mononuclearcell in blood, including tube body, piston and push rod, push rod are threadedly coupled with tube body, and piston is fixedly connected with push rod；Tube body is divided into area under control, extracts area and laydown area, extracts area between upper area under control and laydown area；Piston is located on the inside of laydown area, and the inner wall sealing of piston and laydown area is slidably connected；Push rod is located at the outside of laydown area far from one end of piston, with piston along the axial movement of laydown area when push rod is rotated relative to tube body；Upper area under control is equipped with the first jack far from one end of laydown area, and upper area under control inner space is connected to by the first jack with exterior space；It extracts and offers stomata in area, extract area inner space and be connected to by stomata with exterior space；Its advantage is that extraction efficiency is high, outside air can be completely cut off, additional separation solution is not necessarily to, blood will not be polluted.
Description
Technical field
The present embodiments relate to medical equipment technical fields, and in particular to one kind is for extracting mononuclearcell in blood
Device.
Background technique
In some medical treatment detections and experimentation, need using blood mononuclear cells as material, usually first to blood
It is separated with tissue fluid etc., by the way that new blood (Cord blood) is obtained the blood rich in mononuclearcell using centrifugation
Slurry, wherein be rich in a variety of growth factors, as the nutrient of autogenous cell, monocyte in peripheral blood or Cord blood at present by
It is developed in treatment tumour immunity clinic adjuvant treatment, a large amount of blood/red blood cells after the mononuclearcell in Cord blood also can be used as
Source.After its way is separation, first through freezing, further mass propgation and assigned after treatment characteristic again until needing
It feeds back in substance.
But current separate mode usually uses syringe or heparin tube, and blood was collected, be then sent in aseptic experiment room into
Row centrifugation obtains, and mixes in separation process or with other additional solution, is centrifuged, and operating device is not closed, and is easy pollution in the process,
The safety of additional solution, environmental requirement is stringent, and blood sampling centrifugally operated must be completed in different device, mistake in summary
Journey, it is difficult to guarantee the quality of rich mononuclearcell blood plasma.
Summary of the invention
For this purpose, the embodiment of the present invention provide it is a kind of for extracting the device of mononuclearcell in blood, in the process without with
Other additional solution mixing, reduce chance contact with outside air or solution, reduce and pollute, with raising by mononuclearcell from
The efficiency and safety separated in blood.
To achieve the goals above, the embodiment of the present invention provides the following technical solutions:
It is a kind of for extracting the device of mononuclearcell in blood, including tube body, piston and push rod, push rod and tube body screw thread
Connection, piston are fixedly connected with push rod；
Tube body is divided into area under control, extracts area and laydown area, extracts area between upper area under control and laydown area；
Piston is located on the inside of laydown area, and the inner wall sealing of piston and laydown area is slidably connected；
Push rod is located at the outside of laydown area far from one end of piston, and band piston is along down tube when push rod is rotated relative to tube body
The axial movement in area；
Upper area under control is equipped with the first jack far from one end of laydown area, and upper area under control inner space is empty by the first jack and outside
Between be connected to；
It extracts and offers stomata in area, extract area inner space and be connected to by stomata with exterior space.
The embodiment of the present invention is further arranged to: the upper area under control is rotatably connected to far from one end of the laydown area
It covers, offers the second jack on upper lid, first jack and the second jack are respectively offset from what upper cover was rotated relative to the tube body
Central axis, for upper cover after tube body rotation, the first jack and the second jack being capable of axially aligning or being staggered along upper area under control.
The embodiment of the present invention is further arranged to: the upper cover is threadedly coupled with the tube body.
The embodiment of the present invention is further arranged to: the fixed lid in the one end of the laydown area far from the upper area under control is connected to bottom
Lid, the push rod are threadedly coupled with bottom cover.
The embodiment of the present invention is further arranged to: the internal diameter for extracting area is less than the upper area under control and the laydown area
Diameter.
The embodiment of the present invention is further arranged to: the volume in the upper area under control is equal with the volume of laydown area.
The embodiment of the present invention is further arranged to: the tube body is transparent.
The embodiment of the present invention is further arranged to: the stomata is located at the extraction area close to one end of the laydown area；
The embodiment of the present invention is further arranged to: the upper area under control is connected to rubber far from one end sealing-plug of the laydown area
Plug.
The embodiment of the present invention is further arranged to: being sealedly and fixedly connected in first jack has rubber stopper.
Advantage of the embodiment of the present invention is: when work, stomata card being opened the syringe needle equipped with blood from the first jack
Be inserted into tube body and by blood injection pipe body, after blood is full of laydown area, block stomata, continue blood injecting tube body until
Blood extracts the syringe needle of syringe full of area and upper area under control is extracted out of tube body, rotates upper cover, inserts the first jack and second
Hole mutually staggers, and blocks the connection in tubular body space and exterior space.Tube body is loaded and is centrifuged into centrifuge, upper tube
Area in centrifugal movement between Pivot axle at a distance from be less than laydown area between Pivot axle at a distance from, plasma density
Less than the density of haemocyte, mononuclearcell density is between blood plasma and red blood cell, and blood is divided into three layers after centrifugation, blood
Slurry is located in upper area under control, and red blood cell is located in laydown area, and mononuclearcell is between blood plasma and red blood cell, a part of single core
Cell also is located in laydown area.Tube body is taken out from centrifuge, towards the direction rotating push rod of precession, piston pushes single core thin
Born of the same parents, which are gradually collected to, extracts area, and the syringe needle of syringe is sequentially passed through the first jack and the second jack stretches to and extracts area, will be single
A nucleus, which is drawn into syringe, to be completed to collect.During separation and collection, it is possible to reduce blood exposure in air, is dropped
Pollution of the low mononuclearcell by air.During extracting mononuclearcell with syringe, syringe needle stretches to the bottom for extracting area
Portion in extraction process, extracts and generates negative pressure inside area, and blood plasma is in self gravity and extracts under the suction function in area gradually towards entrance
The direction for extracting area, which is moved to, to be extracted in area, is collected mononuclearcell more thorough, is improved extraction efficiency.
Detailed description of the invention
It, below will be to embodiment party in order to illustrate more clearly of embodiments of the present invention or technical solution in the prior art
Formula or attached drawing needed to be used in the description of the prior art are briefly described.It should be evident that the accompanying drawings in the following description is only
It is merely exemplary, it for those of ordinary skill in the art, without creative efforts, can also basis
The attached drawing of offer, which is extended, obtains other implementation attached drawings.
Structure depicted in this specification, ratio, size etc., only to cooperate the revealed content of specification, for
Those skilled in the art understands and reads, and is not intended to limit the invention enforceable qualifications, therefore does not have technical
Essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size are not influencing the function of the invention that can be generated
Under effect and the purpose that can reach, should all still it fall in the range of disclosed technology contents obtain and can cover.
Fig. 1 is cross-sectional view of the embodiment of the present invention 1 along body central axis cutting；
Fig. 2 is the schematic diagram that connection structure between rubber stopper and tube body is embodied in the embodiment of the present invention 2；
Fig. 3 is the structural schematic diagram of the embodiment of the present invention 3.
In figure:
1, tube body；11, upper area under control；12, area is extracted；13, laydown area；14, the first jack；15, stomata；2, piston；3, it pushes away
Bar；4, upper cover；41, the second jack；5, bottom cover；6, rubber stopper.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily, it is clear that described embodiment is the present invention one
Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not doing
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
Embodiment 1
It is a kind of for extracting the device of mononuclearcell in blood, as shown in Figure 1, include tube body 1, piston 2 and push rod 3,
Push rod 3 is threadedly coupled with tube body 1, and piston 2 is fixedly connected with push rod 3；Tube body 1 divides for upper area under control 11, extracts area 12 and laydown area
13, area 12 is extracted between upper area under control 11 and laydown area 13；Piston 2 is located at 13 inside of laydown area, piston 2 and laydown area 13
Inner wall sealing is slidably connected；Push rod 3 is located at the outside of laydown area 13 far from one end of piston 2, and push rod 3 is rotated relative to tube body 1
When with piston 2 along the axial movement of laydown area 13；Upper area under control 11 is equipped with the first jack 14, upper tube far from one end of laydown area 13
11 inner space of area is connected to by the first jack 14 with exterior space；It extracts and offers stomata 15 in area 12, extract inside area 12
Space is connected to by stomata 15 with exterior space.
When work, stomata 15 is blocked to the syringe for opening (do not need using when stomata 15 use plug) will be equipped with blood
Syringe needle is inserted into tube body 1 from the first jack 14 and injects blood in tube body 1, after blood is full of laydown area 13, blocks stomata 15,
Continue blood injecting tube body 1 until blood pulls out the syringe needle of syringe full of area 12 and upper area under control 11 is extracted out of tube body 1
Out, upper cover 4 is rotated, the first jack 14 and the second jack 41 are mutually staggered, blocks the company of tube body 1 inner space and exterior space
It is logical.Tube body 1 is loaded and is centrifuged into centrifuge, upper area under control 11 in centrifugal movement between Pivot axle at a distance from it is small
In the distance between laydown area 13 and Pivot axle, plasma density is less than the density of haemocyte, mononuclearcell density between
Between blood plasma and red blood cell, blood is divided into three layers after centrifugation, and blood plasma is located in upper area under control 11, and red blood cell is located at laydown area 13
Interior, between blood plasma and red blood cell, a part of mononuclearcell also is located in laydown area 13 mononuclearcell.By tube body 1 from
It is taken out in centrifuge, towards the direction rotating push rod 3 of precession, piston pushes mononuclearcell to be gradually collected to and extracts area 12, will infuse
The syringe needle of emitter sequentially passes through the first jack 14 and the second jack 41 stretches to and extracts area, and mononuclearcell is drawn to syringe
Middle completion is collected.During separation and collection, it is possible to reduce blood exposure in air, reduces mononuclearcell by air
Pollution.During extracting mononuclearcell with syringe, syringe needle stretches to the bottom for extracting area 12, in extraction process, extracts
Negative pressure is generated inside area 2, blood plasma is gradually moved towards the direction for entering extraction area 2 under the suction function in self gravity and extraction area 2
It moves to extracting in area, collects mononuclearcell more thorough, improve extraction efficiency.
Upper area under control 11 is rotatably connected to upper cover 4 far from one end of laydown area 13, and the second jack 41 is offered in upper cover 4, the
One jack 14 and the second jack 41 are respectively offset from the central axis that upper cover 4 is rotated relative to tube body 1, and upper cover 4 is rotated relative to tube body 1
Afterwards, the first jack 14 and the second jack 41 being capable of axially aligning or being staggered along upper area under control 11.
Upper cover 4 is threadedly coupled with tube body 1.The fixed lid in the one end of laydown area 13 far from upper area under control 11 is connected to bottom cover 5, push rod 3
It is threadedly coupled with bottom cover 5.
The internal diameter for extracting area 12 is less than the diameter of upper area under control 11 and laydown area 13.The appearance with laydown area 13 in upper area under control 11
Product is equal.Tube body 1 is transparent.Stomata 15, which is located at, extracts area 12 close to one end of laydown area 13.
Embodiment 2
It is a kind of for extracting the device of mononuclearcell in blood, as shown in Fig. 2, difference from example 1 is that,
Upper cover 4 is rubber stopper 6, and 6 sealing-plug of rubber stopper connects at the upper one end of area under control 11 far from laydown area 13, work, by syringe
Syringe needle is inserted through rubber stopper 6 to protrude into tube body 1, and syringe needle is inserted into tube body 1 or from tube body 1 in withdrawal process, rubber
Rubber plug 6 can be avoided outside air and enter tubular body, and the blood in tube body 1 is avoided to be contaminated.
Embodiment 3
It is a kind of for extracting the device of mononuclearcell in blood, as shown in figure 3, difference from example 1 is that,
Being sealedly and fixedly connected in first jack 14 has rubber stopper 6.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (10)
1. it is a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: including tube body (1), piston (2) and push rod
(3), push rod (3) is threadedly coupled with tube body (1), and piston (2) is fixedly connected with push rod (3)；
Tube body (1) is divided into area under control (11), extracts area (12) and laydown area (13), extraction area (12) be located at upper area under control (11) and under
Between area under control (13)；
Piston (2) is located on the inside of laydown area (13), and piston (2) and the inner wall sealing of laydown area (13) are slidably connected；
Push rod (3) is located at the outside of laydown area (13), band when push rod (3) is rotated relative to tube body (1) far from the one end of piston (2)
Axial movement of the piston (2) along laydown area (13)；
Upper area under control (11) is equipped with the first jack (14) far from the one end of laydown area (13), and upper area under control (11) inner space passes through first
Jack (14) is connected to exterior space；
It extracts and is offered stomata (15) on area (12), extract area (12) inner space and be connected to by stomata (15) with exterior space.
2. it is according to claim 1 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: the upper tube
Area (11) is rotatably connected to upper cover (4) far from the one end of the laydown area (13), and the second jack (41) are offered in upper cover (4),
First jack (14) and the second jack (41) are respectively offset from the central axis that upper cover (4) is rotated relative to the tube body (1), on
Cover after (4) rotate with respect to tube body (1), the first jack (14) and the second jack (41) can along upper area under control (11) axially align or
It is staggered.
3. it is according to claim 2 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: the upper cover
(4) it is threadedly coupled with the tube body (1).
4. it is according to claim 1 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: the down tube
The fixed lid in the one end of area (13) far from the upper area under control (11) is connected to bottom cover (5), and the push rod (3) is threadedly coupled with bottom cover (5).
5. it is according to claim 1 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: the extraction
The internal diameter in area (12) is less than the diameter of the upper area under control (11) and the laydown area (13).
6. it is according to claim 1 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: the upper tube
The volume in area (11) is equal with the volume of laydown area (13).
7. it is according to claim 1 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: the tube body
(1) transparent.
8. it is according to claim 1 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: the stomata
(15) it is located at extraction area (12) close to the one end of the laydown area (13).
9. it is according to claim 1 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: the upper tube
Area (11) is connected to rubber stopper (6) far from one end sealing-plug of the laydown area (13).
10. it is according to claim 2 a kind of for extracting the device of mononuclearcell in blood, it is characterized in that: described
Being sealedly and fixedly connected in one jack (41) has rubber stopper (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910214563.3A CN109794082A (en) | 2019-03-20 | 2019-03-20 | It is a kind of for extracting the device of mononuclearcell in blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910214563.3A CN109794082A (en) | 2019-03-20 | 2019-03-20 | It is a kind of for extracting the device of mononuclearcell in blood |
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| Publication Number | Publication Date |
|---|---|
| CN109794082A true CN109794082A (en) | 2019-05-24 |
Family
ID=66563785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910214563.3A Pending CN109794082A (en) | 2019-03-20 | 2019-03-20 | It is a kind of for extracting the device of mononuclearcell in blood |
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| Country | Link |
|---|---|
| CN (1) | CN109794082A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050186120A1 (en) * | 2002-05-03 | 2005-08-25 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
| TW201231087A (en) * | 2011-01-27 | 2012-08-01 | Maria Von Med Biotechnology Co Ltd | Manufacturing process of skin care product containing multiple growth factors |
| TWM475927U (en) * | 2013-12-17 | 2014-04-11 | Maria Von Med-Biotechnology Co Ltd | Device for isolating plasma from whole blood samples |
| CN107034128A (en) * | 2017-06-16 | 2017-08-11 | 何向锋 | A kind of mononuclearcell separating pipe and its application method |
| CN209790967U (en) * | 2019-03-20 | 2019-12-17 | 林侯汎 | Device for extracting mononuclear cells in blood |
-
2019
- 2019-03-20 CN CN201910214563.3A patent/CN109794082A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050186120A1 (en) * | 2002-05-03 | 2005-08-25 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
| TW201231087A (en) * | 2011-01-27 | 2012-08-01 | Maria Von Med Biotechnology Co Ltd | Manufacturing process of skin care product containing multiple growth factors |
| TWM475927U (en) * | 2013-12-17 | 2014-04-11 | Maria Von Med-Biotechnology Co Ltd | Device for isolating plasma from whole blood samples |
| CN107034128A (en) * | 2017-06-16 | 2017-08-11 | 何向锋 | A kind of mononuclearcell separating pipe and its application method |
| CN209790967U (en) * | 2019-03-20 | 2019-12-17 | 林侯汎 | Device for extracting mononuclear cells in blood |
Non-Patent Citations (3)
| Title |
|---|
| 李大魁: "《中华医学百科全书 临床药学》", 31 December 2018, 中国协和医科大学出版社 * |
| 杨晓清等: "《包装机械与设备》", 31 March 2009, 国防工业出版社 * |
| 谭李红等: "《实用脑血管病介入诊断治疗学》", 31 August 2011, 科学技术文献出版社 * |
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Application publication date: 20190524 |
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