CN109730088A - A kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof - Google Patents
A kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof Download PDFInfo
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- CN109730088A CN109730088A CN201910074171.1A CN201910074171A CN109730088A CN 109730088 A CN109730088 A CN 109730088A CN 201910074171 A CN201910074171 A CN 201910074171A CN 109730088 A CN109730088 A CN 109730088A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention belongs to microorganism formulation technical field, a kind of with being related to improvement stony desertification microorganism formulation of plant growth and preparation method thereof；Microorganism formulation includes 40-60 parts of starch, 10-20 parts of charcoal, 2-5 parts of humic acid, 8-10 parts of microbial bacterial agent, 15-30 parts of algal polysaccharides；Microbial bacterial agent is the brevibacterium Brevibacterium sp.TS22 tamed by low altitude area and room temperature, deposit number are as follows: CGMCC 16576；Microbial bacterial agent may also include phosphate solubilizing bacteria, and weight is the 0~20% of microbial bacterial agent；The preparation step of microorganism formulation is as follows: the starting components of microorganism formulation being put into blender according to parts by weight, are stirred to react, microorganism formulation is obtained；Microorganism formulation of the invention can individually or agroforestry organic fertilizer uses；Operation of the present invention is easy, and production cost is low, so that the emergence rate of desertification plant improves 50% or more.
Description
Technical field
The invention belongs to plant growth microorganism formulation technical fields, and in particular to a kind of improvement stony desertification ground plant growth
Microorganism formulation and preparation method thereof.
Background technique
Stony desertification, also known as land desertification, refer to causes surface soil to lose because of soil erosion, exposed bedrock, soil
The phenomenon that losing agricultural use value and environmental degradation.Stony desertification soil thickness is thin (most less than 10 centimetres), and earth's surface is in
Now similar to the rock of desertscape gradually exposed evolution process.For the origin cause of formation, it is artificial for leading to the principal element of stony desertification
Activity.Since natural vegetation is constantly destroyed for a long time, the abrupt slope of large area is opened up wasteland, and causes earth's surface exposed, basement rock exposure,
Heavy rain scouring force is strong, and rock is gradually shown especially exposed after a large amount of soil erosion, to show stony desertification phenomenon.Stony desertification is
As serious ecological problem, referred to as " cancer of the earth ".
On the one hand stony desertification soil can reduce the germination percentage of crop.On the other hand, calcium ion, salt ion etc. are dense in stony desertification soil
Degree is higher, and calcium ion, the salt ion of high concentration easily cause soil water potential to decline, therefore generates water stress to plant, simultaneously also
Ionic stress is generated, intracellular ionic equilibrium is destroyed；The accumulation of active oxygen in plant is also resulted in, to injure cell very
Extremely it is killed；In addition, stony desertification soil also makes plant generate Nutrient Stress, the growth and development of such plant will be abnormal, right
The overall performance of plant growth and development is to inhibit the growth of tissue and organ, and accelerated development process shortens nutrient growth and reproduction
Phase.
So being attempted to develop and utilize stony desertification soil from the directions such as agricultural, chemistry, biology；It is arranged according to improvement
The difference applied can obtain different effects for the development and utilization on stony desertification ground.Improvement stony desertification soil can pass through desalinization of soil by flooding or leaching etc.
Measure, or its ion etc. is improved with crops such as green manuring, organic fertilizer or kind rice；These methods are to stony desertification
Although soil improvement have certain effect, effect is unstable, and in practical application, a large amount of human and material resources and
The cost of this measure has virtually been significantly greatly increased in the investment of financial resources.
Summary of the invention
In view of the above-mentioned problems, present invention seek to address that one of described problem, a kind of improvement stony desertification is provided plant growth
Microorganism formulation and preparation method thereof, specific technical solution is as follows:
A kind of present invention firstly provides improvement stony desertification the microorganism formulation of plant growth, including following starting components
And parts by weight, 40-60 parts of starch, 10-20 parts of charcoal, 2-5 parts of humic acid, 8-10 parts of microbial bacterial agent, algal polysaccharides 15-
30 parts.
The microbial bacterial agent is the brevibacterium (Brevibacterium sp.TS22) derived from Qinghai-Tibet Platean through too low sea
It pulls out and tames and obtain with room temperature, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows:
CGMCC 16576。
The mode of the low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture
In base, in secondary culture repeatedly under room temperature, the brevibacterium for adapting to low altitude area and normal temperature condition is obtained.
The LB culture medium includes tryptone 10g/L, yeast extract 5g/L, sodium chloride 5g/L, pH 7.4.
The microbial bacterial agent further includes phosphate solubilizing microorganism, and the phosphate solubilizing microorganism is bacillus megaterium (Bacillus
Megaterium), pseudomonad (Pseudomonas sp.) or agrobacterium (Agrobacterium sp.).
Need to tame culture before the phosphate solubilizing bacteria use；The domestication culture is that phosphorus decomposing bacterial strain is inoculated into Phos training
Base is supported, in 20-25 DEG C；Strain passage culture is carried out under low temperature environment；The Phos culture medium prescription are as follows: glucose 10g/L,
(NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L,
MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of sterilizing 20min of 5g/L, pH.
The phosphate solubilizing microorganism after the brevibacterium and domestication culture of low altitude area and room temperature domestication acquisition is when in use
It needs to be tamed with the soil environment of use environment.
It is described by low altitude area and room temperature domestication obtain brevibacterium and domestication culture after phosphate solubilizing microorganism when in use,
Taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed need to be passed through are as follows: low altitude area will be passed through
The brevibacterium tamed with room temperature connects and the phosphate solubilizing microorganism after domestication culture, is inoculated into LB culture medium respectively, in 20-25 DEG C
Under secondary culture repeatedly；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L,
PH to 7.4,121 DEG C of high pressure steam sterilization 20min are adjusted with NaOH.
The weight of the phosphate solubilizing bacteria is the 0-20% of microbial bacterial agent.
A kind of the present invention also provides improvement stony desertification the preparation method of the microorganism formulation of plant growth, specific steps are such as
Under:
The starting components of microorganism formulation are put into blender according to parts by weight, the revolving speed that blender is arranged exists
Room temperature item is reacted, and the microorganism formulation of with improving stony desertification plant growth is obtained.
The revolving speed of the blender is set as 100-300r/min；Reaction time is 10-30min
The microorganism formulation can also be used in mixed way with agricultural organic fertilizer.
Beneficial effects of the present invention:
(1) microorganism formulation prepared by the present invention with being effectively improved stony desertification plant growth；It adds at microorganism formulation
After reason, the emergence rate of plant can be improved 50% or more, and the biomass of plant improves 38% or more；In addition, prepared by the present invention
Microorganism formulation can also be used in combination with agricultural organic fertilizer, can preferably improve the growth of plant.
(2) operation of the present invention is easy, and production cost is low, has highly important theory and practice meaning.
Specific embodiment
In the present invention, the number of bacillus megaterium is CICC20665, is purchased from the management of Chinese industrial Microbiological Culture Collection
Center；The number of pseudomonad is CGMCC10242, purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms
The heart；The number of agrobacterium is CICC10214, is purchased from Chinese industrial Microbiological Culture Collection administrative center；
Brevibacterium Brevibacterium sp.TS22, it is commonly micro- to be preserved in China Committee for Culture Collection of Microorganisms
Bio-Centers, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Deposit number are as follows: CGMCC 16576；Preservation date: 2018
On October 12, in.
Brevibacterium sp.TS22 sequence information:
>CCCCGCCATCGGCTTCGTTCGAATGCCATGCATAGCGACTCGCACGCCAAGCGTG CGTCCCTGAGC
CATGATCAAACTCTATGAGTAACACGTGGGCAACCTGCCTATAAGACT GGGATAACTTCGGGAAACCGGAGCTAA
TACCGGATACGTTCTTTTCTCGCATGAGAGAA GATGGAAAGACGGTTTACGCTGTCACTTATAGATGGGCCCGCG
GCGCATTAGCTAGTTG GTGAGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCC
ACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCC GCAATGGACGAAAGTCT
GACGGAGCAACGCCGCGTGAACGAAGAAGGCCTTCGGGTC GTAAAGTTCTGTTGTTAGGGAAGAACAAGTACCAG
AGTAACTGCTGGTACCTTGACGGT ACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACG
TAGGTGGC AAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTCCTTAAGTCTGATG TGAAAGC
CCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGA AGAGGAAAGTGGAATTCCAAAGTG
TAGCGGTGAAATGCGTAGAGATTTGGAGGAACAC CAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAG
GCGCGAAAGCGTGGGGA GCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAG
AGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACG GCCGCAAGGCTGAAAC
TCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT GGTTTAATTCGAAGCAACGCGAAGAACCTTACC
AGGTCTTGACATCCTCTGACAACCCT AGAGATAGGGCTTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGG
TTGTCGTCAG CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTG CCAGC
ATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGG GATGACGTCAAATCATCATGCC
CCTTATGACCTGGGCTACACACGTGCTACAATGGATGG TACAAAGGGCTGCAAACCTGCGAAGGTAAGCGAATCC
CATAAAGCCATTCTCAGTTCGG ATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGAT
CAGCAT GCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCC
Embodiment 1:
A kind of microorganism formulation of with improving stony desertification plant growth, including following starting components and parts by weight, form sediment
60 parts of powder, 10 parts of charcoal, 2 parts of humic acid, 18 parts of algal polysaccharides, 10 parts of microbial bacterial agent, microbial bacterial agent further include domestication
Bacillus megaterium after culture, weight are the 10% of microbial bacterial agent；
Microbial bacterial agent be derived from Qinghai-Tibet Platean brevibacterium (Brevibacterium sp.TS22) by low altitude area and
Room temperature is tamed and is obtained, and is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC
16576；The mode of low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture medium,
In secondary culture repeatedly under room temperature, the brevibacterium for adapting to low altitude area and normal temperature condition is obtained；
The domestication culture of bacillus megaterium is to be inoculated into Phos culture medium, and strain passage training is carried out under 20 environment
It supports；The Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O
0.5g/L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)25g/L, pH 7.2,115
DEG C sterilizing 20min.
Bacillus megaterium is using after the brevibacterium obtained by low altitude area and room temperature domestication and domestication culture
When, taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed need to be passed through are as follows: will be through too low sea
The brevibacterium tamed with room temperature and the bacillus megaterium after domestication culture are pulled out, is inoculated into LB culture medium respectively, in 20-25
Secondary culture repeatedly at DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/
L adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method of with the improving stony desertification microorganism formulation of plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 180r/min, and temperature is room temperature, reaction
Time is 22min, and the microorganism formulation of with improving stony desertification plant growth is made.Microorganism formulation and agricultural organic fertilizer when application
It is uniformly mixed by weight for 1:99.
Embodiment 2:
A kind of microorganism formulation of with improving stony desertification plant growth, including following starting components and parts by weight, form sediment
42 parts of powder, 15 parts of charcoal, 5 parts of humic acid, 30 parts of algal polysaccharides, 8 parts of microbial bacterial agent, microbial bacterial agent further include domestication training
Agrobacterium after supporting, weight are the 20% of microbial bacterial agent.；The microbial bacterial agent is the brevibacterium derived from Qinghai-Tibet Platean
(Brevibacterium sp.TS22) is obtained by low altitude area and room temperature domestication, is preserved in Chinese microorganism strain preservation management
Committee's common micro-organisms center, deposit number are as follows: CGMCC 16576；The mode of low altitude area and room temperature domestication are as follows: blueness will be isolated from
The brevibacterium of hiding plateau soil is inoculated into LB culture medium, in secondary culture repeatedly under room temperature, obtain adapting to low altitude area and
The brevibacterium of normal temperature condition；
The domestication culture of agrobacterium is to be inoculated into Phos culture medium, and strain passage culture is carried out under 25 DEG C of environment；
The Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/
L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of 5g/L, pH sterilizings
20min；
It is described to tame the brevibacterium obtained by low altitude area and room temperature and tame the agrobacterium after cultivating when in use,
It need to be tamed again by corresponding with area's main environmental conditions will be used condition, then be respectively as follows: will be through too low for the condition tamed
The brevibacterium of height above sea level and room temperature domestication is inoculated into LB culture medium, the secondary culture repeatedly at 20-25 DEG C；It will be by domestication culture
Inoculating agrobacteria is into LB culture medium afterwards, the secondary culture repeatedly at 10-20 DEG C；Wherein LB culture medium prescription is as follows: pancreas egg
White peptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilizations with NaOH
20min。
A kind of preparation method of with the improving stony desertification microorganism formulation of plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 200r/min, and temperature is room temperature, reaction
Time is 20min, and the microorganism formulation of with improving stony desertification plant growth is made.Microorganism formulation and agricultural organic fertilizer when application
It is uniformly mixed by weight for 2:98.
Embodiment 3:
A kind of microorganism formulation of with improving stony desertification plant growth, including following starting components and parts by weight, form sediment
58 parts of powder, 12 parts of charcoal, 2 parts of humic acid, 20 parts of algal polysaccharides, 8 parts of microbial bacterial agent, microbial bacterial agent further include domestication training
Bacillus megaterium after supporting, weight are the 20% of microbial bacterial agent；The microbial bacterial agent is the quarter butt derived from Qinghai-Tibet Platean
Bacterium (Brevibacterium sp.TS22) obtains by low altitude area and room temperature domestication, is preserved in Chinese microorganism strain preservation
Administration committee's common micro-organisms center, deposit number are as follows: CGMCC 16576；The mode of low altitude area and room temperature domestication are as follows: will separate
It is inoculated into LB culture medium from the brevibacterium of Qinghai-Tibetan plateau soil, in secondary culture repeatedly under room temperature, obtains adapting to low sea
Pull out the brevibacterium with normal temperature condition；
The domestication culture of bacillus megaterium is to be inoculated into Phos culture medium, and strain passage training is carried out under 20 environment
It supports；The Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O
0.5g/L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)25g/L, pH 7.2,115
DEG C sterilizing 20min.
Bacillus megaterium is using after the brevibacterium obtained by low altitude area and room temperature domestication and domestication culture
When, taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed need to be passed through are as follows: will be through too low sea
The brevibacterium tamed with room temperature and the bacillus megaterium after domestication culture are pulled out, is inoculated into LB culture medium respectively, in 20-25
Secondary culture repeatedly at DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/
L adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method of with the improving stony desertification microorganism formulation of plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 100r/min, and temperature is room temperature, reaction
Time is 30min, and the microorganism formulation of with improving stony desertification plant growth is made.
Embodiment 4:
A kind of microorganism formulation of with improving stony desertification plant growth, including following starting components and parts by weight, form sediment
55 parts of powder, 20 parts of charcoal, 3 parts of humic acid, 17 parts of algal polysaccharides, 5 parts of microbial bacterial agent, microbial bacterial agent further include domestication training
Pseudomonad after supporting, weight are the 10% of microbial bacterial agent；The microbial bacterial agent is the brevibacterium derived from Qinghai-Tibet Platean
(Brevibacterium sp.TS22) is obtained by low altitude area and room temperature domestication, is preserved in Chinese microorganism strain preservation management
Committee's common micro-organisms center, deposit number are as follows: CGMCC 16576；The mode of low altitude area and room temperature domestication are as follows: blueness will be isolated from
The brevibacterium of hiding plateau soil is inoculated into LB culture medium, in secondary culture repeatedly under room temperature, obtain adapting to low altitude area and
The brevibacterium of normal temperature condition；
The domestication culture of pseudomonad is to be inoculated into Phos culture medium, and strain passage culture is carried out under 20 environment；Institute
State Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/L,
KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of 5g/L, pH sterilizings
20min。
It is described to tame the brevibacterium obtained by low altitude area and room temperature and tame the pseudomonad after cultivating when in use,
It need to be tamed again by corresponding with area's main environmental conditions will be used condition, then be respectively as follows: will be through too low for the condition tamed
The brevibacterium of height above sea level and room temperature domestication is inoculated into LB culture medium, the secondary culture repeatedly at 20-25 DEG C；It will be by domestication culture
Pseudomonad afterwards is inoculated into LB culture medium, the secondary culture repeatedly at 10-20 DEG C；Wherein LB culture medium prescription is as follows: pancreas
Peptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilizations with NaOH
20min。
A kind of preparation method of with the improving stony desertification microorganism formulation of plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 250r/min, and temperature is room temperature, reaction
Time is 15min, and the microorganism formulation of with improving stony desertification plant growth is made.
Embodiment 5:
A kind of microorganism formulation of with improving stony desertification plant growth, including following starting components and parts by weight, form sediment
53 parts of powder, 15 parts of charcoal, 3 parts of humic acid, 9 parts of microbial bacterial agent, 20 parts of algal polysaccharides, the microbial bacterial agent are derived from blueness
The brevibacterium (Brevibacterium sp.TS22) on hiding plateau obtains by low altitude area and room temperature domestication, is preserved in the micro- life of China
Object culture presevation administration committee common micro-organisms center, deposit number are as follows: CGMCC 16576；The side of low altitude area and room temperature domestication
Formula are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture medium, in secondary culture repeatedly under room temperature, obtains
To the brevibacterium for adapting to low altitude area and normal temperature condition；
The brevibacterium obtained by low altitude area and room temperature domestication when in use, need to pass through and will use the main ring in area
Condition corresponding condition in border is tamed again, then the condition tamed are as follows: is inoculated into the brevibacterium tamed by low altitude area and room temperature
In LB culture medium, the secondary culture repeatedly at 20-25 DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast mention
Object 5g/L, sodium chloride 10g/L are taken, adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method of with the improving stony desertification microorganism formulation of plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 280r/min, and temperature is room temperature, reaction
Time is 12min, and the microorganism formulation of with improving stony desertification plant growth is made.
Embodiment effect:
Field trial is to fertilizer functional verification:
Summer in choosing the more consistent stony desertification experimental plot of condition in Guilin, is divided into 18 1 meter × 1 meters
Block is tested each embodiment in plot and is used identical part respectively by above-mentioned 5 embodiments and each three design iterations of blank control
Amount (i.e. 100 grams) is uniformly blended into soil, sows each 100, green vegetables seed, and normal watering Irrigation counts emergence rate after 10 days, then
Thinning after 10 days respectively stays 50 plants；Harvest randomly chooses each 20 plants of titles gross weight after 30 days again.Control group does not add microorganism formulation
It is handled with agricultural organic fertilizer, remaining step is identical.Statistical result is described in table 1 below.
1 embodiment effect statistical form of table
As can be seen that the effect of embodiment 1-5 is substantially better than the effect of control group from above-mentioned table 1；The microorganism system of addition
After agent processing, the emergence rate of plant can be improved 50% or more, and the biomass of plant improves 38% or more, this explanation is in the present invention
Formula under can reach promote stony desertification plant growth.In addition, from 1 result of table it has also been discovered that being properly added agricultural has
Machine fertilizer can preferably improve the growth-promoting effect of plant；The emergence rate of plant and the biomass of plant improve 61% or so.
Illustrate: above embodiments are only to illustrate the present invention and not limit the technical scheme described by the invention；Therefore,
Although this specification is referring to above-mentioned each embodiment, the present invention has been described in detail, the common skill of this field
Art personnel should be appreciated that and still can modify to the present invention or equivalent replacement；And all do not depart from spirit of the invention and
The technical solution and its improvement of range, should all cover in scope of the presently claimed invention.
Sequence table
<110>Jiangsu University
<120>a kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 2
<211> 1384
<212> DNA
<213>brevibacterium (Brevibacterium sp.)
<400> 2
ccccgccatc ggcttcgttc gaatgccatg catagcgact cgcacgccaa gcgtgcgtcc 60
ctgagccatg atcaaactct atgagtaaca cgtgggcaac ctgcctataa gactgggata 120
acttcgggaa accggagcta ataccggata cgttcttttc tcgcatgaga gaagatggaa 180
agacggttta cgctgtcact tatagatggg cccgcggcgc attagctagt tggtgaggta 240
atggctcacc aaggcgacga tgcgtagccg acctgagagg gtgatcggcc acactgggac 300
tgagacacgg cccagactcc tacgggaggc agcagtaggg aatcttccgc aatggacgaa 360
agtctgacgg agcaacgccg cgtgaacgaa gaaggccttc gggtcgtaaa gttctgttgt 420
tagggaagaa caagtaccag agtaactgct ggtaccttga cggtacctaa ccagaaagcc 480
acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggaatt 540
attgggcgta aagcgcgcgc aggtggttcc ttaagtctga tgtgaaagcc cacggctcaa 600
ccgtggaggg tcattggaaa ctggggaact tgagtgcaga agaggaaagt ggaattccaa 660
agtgtagcgg tgaaatgcgt agagatttgg aggaacacca gtggcgaagg cgactttctg 720
gtctgtaact gacactgagg cgcgaaagcg tggggagcaa acaggattag ataccctggt 780
agtccacgcc gtaaacgatg agtgctaagt gttagagggt ttccgccctt tagtgctgca 840
gctaacgcat taagcactcc gcctggggag tacggccgca aggctgaaac tcaaaggaat 900
tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 960
ttaccaggtc ttgacatcct ctgacaaccc tagagatagg gctttcccct tcgggggaca 1020
gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080
gcaacgagcg caacccttga tcttagttgc cagcattcag ttgggcactc taaggtgact 1140
gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200
gggctacaca cgtgctacaa tggatggtac aaagggctgc aaacctgcga aggtaagcga 1260
atcccataaa gccattctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga 1320
atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380
cgcc 1384
Claims (9)
1. a kind of ecological agent of with improving stony desertification plant growth, which is characterized in that including following starting components and weight
Number, 40-60 parts of starch, 10-20 parts of charcoal, 2-5 parts of humic acid, 8-10 parts of microbial bacterial agent, 15-30 parts of algal polysaccharides；
The microbial bacterial agent is the brevibacterium Brevibacterium sp.TS22 derived from Qinghai-Tibet Platean, is tamed and dociled by low altitude area and room temperature
Change and obtain, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC 16576.
2. a kind of ecological agent of with improving stony desertification plant growth according to claim 1, which is characterized in that described low
The condition of height above sea level and room temperature domestication are as follows: brevibacterium Brevibacterium sp.TS22 is inoculated into LB culture medium, in room temperature
Under the conditions of secondary culture repeatedly；The LB culture medium includes tryptone 10g/L, yeast extract 5g/L, sodium chloride 5g/L, pH
It is 7.4.
3. a kind of ecological agent of with improving stony desertification plant growth according to claim 1, which is characterized in that described micro-
Bacteria agent further includes the phosphate solubilizing bacteria after domestication culture, and the weight of phosphate solubilizing bacteria is the 0- of microbial bacterial agent after the domestication culture
20%；The domestication culture is that phosphorus decomposing bacterial strain is inoculated into Phos culture medium, and bacterial strain biography is carried out under 10-20 DEG C of low temperature environment
It is commissioned to train feeding；The Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O
0.5g/L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)25g/L, pH 7.2,115
DEG C sterilizing 20min.
4. a kind of ecological agent of with improving stony desertification plant growth according to claim 3, which is characterized in that the solution
Phosphorus bacterium is bacillus megaterium, pseudomonad or agrobacterium.
5. a kind of ecological agent of with improving stony desertification plant growth according to claim 2 or 3, which is characterized in that institute
Stating the phosphate solubilizing bacteria after the brevibacterium and domestication culture of low altitude area and room temperature domestication acquisition when in use, need to pass through and will make
With taming again for the corresponding condition of area's main environmental conditions, then the condition tamed are as follows: short by being tamed by low altitude area and room temperature
Bacillus and the phosphate solubilizing bacteria after domestication culture, are inoculated into LB culture medium, the secondary culture repeatedly at 20-25 DEG C respectively；Wherein
LB culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L, adjusts pH to 7.4 with NaOH,
121 DEG C of high pressure steam sterilization 20min.
6. a kind of preparation method of with improving stony desertification ecological agent of plant growth according to claim 1, feature
It is, the specific steps are as follows: the starting components of ecological agent are put into blender according to parts by weight, blender is set
Revolving speed reacted in room temperature item, obtain improve stony desertification plant growth ecological agent.
7. a kind of preparation method of with improving stony desertification ecological agent of plant growth according to claim 6, feature
It is, the revolving speed of the blender is set as 100-300r/min；Reaction time is 10-30min.
8. by a kind of ecological agent with being used to improve stony desertification plant of with improving stony desertification plant growth described in claim 1
The purposes of growth, which is characterized in that the ecological agent of with the improving stony desertification plant growth is used alone or agroforestry has
Machine fertilizer uses.
9. purposes according to claim 8, which is characterized in that the ecological agent of with the improving stony desertification plant growth is multiple
Agricultural organic fertilizer is closed in use, the ecological agent of with improving stony desertification plant growth and the mass ratio of agricultural organic fertilizer are 1-2:
98-99。
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109735465A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof |
| CN109735466A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof |
| CN114231458A (en) * | 2021-12-24 | 2022-03-25 | 青岛尚德生物技术有限公司 | Compound microbial agent for improving sugar-acid ratio of melons and fruits as well as preparation method and application of compound microbial agent |
| EP4219433A1 (en) * | 2022-01-27 | 2023-08-02 | Farment Bio Solutions Ltd | Bio-stimulant and method of producing same |
Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699298A (en) * | 2004-05-21 | 2005-11-23 | 汉城精细技术有限公司 | PGPR growth accelerant and method for culturing agronomic crop using it |
| CN101671205A (en) * | 2009-09-27 | 2010-03-17 | 上海创博生物技术有限公司 | Composite microbial preparation for promoting growth of rape and preparation method thereof |
| CN101801891A (en) * | 2007-06-20 | 2010-08-11 | 欧亚生物科学有限公司 | Microbial formulation and method of using the same promote plant growth |
| WO2012038740A1 (en) * | 2010-09-21 | 2012-03-29 | Sita Logistics Limited | Soil treatment process |
| CN104478498A (en) * | 2014-12-05 | 2015-04-01 | 上海创博生态工程有限公司 | Microbial preparation for ecological restoration of medium and low-yield field and preparation method of microbial preparation |
| CN105295928A (en) * | 2015-11-27 | 2016-02-03 | 上海师范大学 | Soil conditioner applicable to deteriorated grassland and preparation method |
| CN105367335A (en) * | 2015-11-19 | 2016-03-02 | 中国科学院南京土壤研究所 | Biochar-based coast saline-alkali land improvement conditioner and preparation method and application thereof |
| CN106278767A (en) * | 2016-08-19 | 2017-01-04 | 杨文霞 | A kind of organic biofertilizer improving heavy metal in soil pollution |
| KR101720958B1 (en) * | 2016-10-10 | 2017-03-29 | 주식회사 포이엔 | Soil-conditioner and method of manufacturing the same |
| CN107236548A (en) * | 2017-07-31 | 2017-10-10 | 钟春明 | Saline-alkali soil land soil agent |
| CN107382496A (en) * | 2017-08-11 | 2017-11-24 | 中澳怡柯唯生物技术研究与发展有限责任公司 | A kind of artificial soil for strengthening crop anti-adversity and the method for strengthening crop anti-adversity |
| CN108046931A (en) * | 2018-01-17 | 2018-05-18 | 河南省亿稼欣生物科技有限公司 | A kind of composite microbiological fertilizer for synergy of losing weight and preparation method thereof |
| US9980912B2 (en) * | 2014-10-01 | 2018-05-29 | Cool Planet Energy Systems, Inc. | Biochars for use with animals |
| CN108586060A (en) * | 2018-06-25 | 2018-09-28 | 山东农业大学 | It is a kind of using polyolefin-wax as the functional form composite coated controlled-release of priming coat fertilizer and its production method |
| CN108637010A (en) * | 2018-03-29 | 2018-10-12 | 上海金禾天地信息科技有限公司 | It is a kind of to significantly reduce the efficient soil remediation method of cadmium, lead content simultaneously |
| CA3011849A1 (en) * | 2017-07-20 | 2019-01-20 | Acterra Tech Ltd. | Bio-stimulant and method of producing same |
-
2019
- 2019-01-25 CN CN201910074171.1A patent/CN109730088A/en active Pending
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699298A (en) * | 2004-05-21 | 2005-11-23 | 汉城精细技术有限公司 | PGPR growth accelerant and method for culturing agronomic crop using it |
| CN101801891A (en) * | 2007-06-20 | 2010-08-11 | 欧亚生物科学有限公司 | Microbial formulation and method of using the same promote plant growth |
| CN101671205A (en) * | 2009-09-27 | 2010-03-17 | 上海创博生物技术有限公司 | Composite microbial preparation for promoting growth of rape and preparation method thereof |
| WO2012038740A1 (en) * | 2010-09-21 | 2012-03-29 | Sita Logistics Limited | Soil treatment process |
| US9980912B2 (en) * | 2014-10-01 | 2018-05-29 | Cool Planet Energy Systems, Inc. | Biochars for use with animals |
| CN104478498A (en) * | 2014-12-05 | 2015-04-01 | 上海创博生态工程有限公司 | Microbial preparation for ecological restoration of medium and low-yield field and preparation method of microbial preparation |
| CN105367335A (en) * | 2015-11-19 | 2016-03-02 | 中国科学院南京土壤研究所 | Biochar-based coast saline-alkali land improvement conditioner and preparation method and application thereof |
| CN105295928A (en) * | 2015-11-27 | 2016-02-03 | 上海师范大学 | Soil conditioner applicable to deteriorated grassland and preparation method |
| CN106278767A (en) * | 2016-08-19 | 2017-01-04 | 杨文霞 | A kind of organic biofertilizer improving heavy metal in soil pollution |
| KR101720958B1 (en) * | 2016-10-10 | 2017-03-29 | 주식회사 포이엔 | Soil-conditioner and method of manufacturing the same |
| CA3011849A1 (en) * | 2017-07-20 | 2019-01-20 | Acterra Tech Ltd. | Bio-stimulant and method of producing same |
| CN107236548A (en) * | 2017-07-31 | 2017-10-10 | 钟春明 | Saline-alkali soil land soil agent |
| CN107382496A (en) * | 2017-08-11 | 2017-11-24 | 中澳怡柯唯生物技术研究与发展有限责任公司 | A kind of artificial soil for strengthening crop anti-adversity and the method for strengthening crop anti-adversity |
| CN108046931A (en) * | 2018-01-17 | 2018-05-18 | 河南省亿稼欣生物科技有限公司 | A kind of composite microbiological fertilizer for synergy of losing weight and preparation method thereof |
| CN108637010A (en) * | 2018-03-29 | 2018-10-12 | 上海金禾天地信息科技有限公司 | It is a kind of to significantly reduce the efficient soil remediation method of cadmium, lead content simultaneously |
| CN108586060A (en) * | 2018-06-25 | 2018-09-28 | 山东农业大学 | It is a kind of using polyolefin-wax as the functional form composite coated controlled-release of priming coat fertilizer and its production method |
Non-Patent Citations (3)
| Title |
|---|
| 孔玮琳等: "水稻秸秆炭及组合改良剂对石漠化地区土壤玉米系统中重金属迁移特征影响", 《环境工程学报》 * |
| 张龙等: "《绿色化学》", 31 August 2014, 华中科技大学出版社 * |
| 彭津琴等: "遏制土壤盐碱化、荒漠化的必要性及技术进展", 《天津农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109735465A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof |
| CN109735466A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof |
| CN114231458A (en) * | 2021-12-24 | 2022-03-25 | 青岛尚德生物技术有限公司 | Compound microbial agent for improving sugar-acid ratio of melons and fruits as well as preparation method and application of compound microbial agent |
| EP4219433A1 (en) * | 2022-01-27 | 2023-08-02 | Farment Bio Solutions Ltd | Bio-stimulant and method of producing same |
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