CN109735465A - A kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof - Google Patents
A kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof Download PDFInfo
- Publication number
- CN109735465A CN109735465A CN201910074222.0A CN201910074222A CN109735465A CN 109735465 A CN109735465 A CN 109735465A CN 201910074222 A CN201910074222 A CN 201910074222A CN 109735465 A CN109735465 A CN 109735465A
- Authority
- CN
- China
- Prior art keywords
- plant growth
- microorganism formulation
- parts
- beach
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fertilizers (AREA)
Abstract
The invention belongs to plant growth microorganism formulation technical fields, and in particular to a kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof.The microorganism formulation of with the improving beach plant growth mainly includes starch, microbial bacterial agent, algal polysaccharides, according to parts by weight, 60-70 parts of starch, 8-10 parts of microbial bacterial agent, 18-28 parts of algal polysaccharides, biodegradable foamed dose 2-5 parts.The present invention also provides the improvement beach the preparation method of the microorganism formulation of plant growth.Improvement beach of the invention plant growth microorganism formulation individually or agroforestry organic fertilizer etc. can in beach be effectively improved plant rhizosphere ecological environment, significantly improve plant growth.
Description
Technical field
The invention belongs to plant growth microorganism formulation technical field, and in particular to a kind of with improving beach plant growth
Microorganism formulation and preparation method thereof.
Background technique
Present beach generally refers to Coastal beach more.China tidal land be mainly distributed on North gets Liaoning, south to Guangdong, Guangxi and
The beach area in Hainan is an important component of littoral zone.217.04 ten thousand hectares of China's Marine shoal gross area.Beach
For the industries important in inhibiting such as fishery cultivating.The development and utilization of shoaly land are with a long history, but tradition development and utilization
To enclose tideland for cultivation based on breeding, development mode is single, level is low, with the development of economy and society, the development and utilization of shoaly land
It increasingly attracts people's attention, gradually becomes the important growth pole of coastal area development regional economy.Therefore, reinforce to edge
The research of the Sustainable Development and Utilization of extra large Tidal Flat Resources is of great practical significance.
Beach not only salt content it is high, partial region oxygen content is also relatively low.And salt damage is 21 century world agriculture
Major issue, and one of the ecocrisis that current China's economic development is faced.Often seriously affect vegetable seeds to beach
It sprouts；And further by influencing CO2It is diffused into binding site, changes the structure and function for being responsible for the organelle of light reaction, changes
The chemical process of dimmed reaction, inhibition assimilation products shift and influence the photosynthetic capacity of plant；In addition Plants under Salt Stress is sharp
Plain adjustment effect and metabolism are also affected, to seriously affect the development and growth of plant.
So being attempted to beach develop and utilize from the directions such as agricultural, chemistry, biology.According to ameliorative measure
Difference can obtain different effects for the development and utilization on beach ground.It can be arranged to improvement beach by draining, desalinization of soil by flooding or leaching etc.
It applies, or its salt is improved with crops such as green manuring, organic fertilizer or kind rice.Improvement of these methods to beach ground
Although there is certain effect, effect is unstable, and in practical application, the throwing of a large amount of human and material resources and financial resources
Enter virtually to be significantly greatly increased the cost of this measure.Changed in recent years by improvement beach geomicrobes community structure and function
With the being apt to beach biological control measures such as plant growth show powerful advantages.
Summary of the invention
In view of this, a kind of present invention with providing improvement beach microorganism formulation of plant growth and preparation method thereof, tool
Body technique scheme is as follows:
A kind of microorganism formulation of with improving beach plant growth, including starch, microbial bacterial agent, algal polysaccharides and biology
Degradable foaming agent, according to parts by weight, 60-70 parts of starch, 8-10 parts of microbial bacterial agent, 18-28 parts of algal polysaccharides, biology can
Foaming agent 2-5 parts of degradation；
The microbial bacterial agent is that the brevibacterium Brevibacterium sp.TS22 derived from Qinghai-Tibet Platean passes through low altitude area
It tames and obtains with room temperature, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is
CGMCC16576, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation time 2018.10.12.
The condition of the low altitude area and room temperature domestication are as follows: train the TS22 strain inoculated for being isolated from Qinghai-Tibetan plateau soil to LB
It supports in base, secondary culture, LB culture medium prescription are as follows repeatedly under the conditions of 20-25 DEG C of room temperature: tryptone 10g/L, yeast mention
Object 5g/L, sodium chloride 10g/L are taken, the medium pH is adjusted with NaOH, reaches 7.4, is settled to 1L with deionized water, it is high
Press steam sterilizing 20min；
In use, taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed must be passed through
Are as follows: by the TS22 strain inoculated tamed by low altitude area and room temperature into LB culture medium, passed repeatedly under the conditions of 20-25 DEG C of room temperature
Be commissioned to train feeding, LB culture medium prescription is as follows: tryptone (10g/L), yeast extract (5g/L), sodium chloride (10g/L) are used
NaOH adjusts the medium pH, reaches 7.4, is settled to 1L, high pressure steam sterilization 20min with deionized water.
The microbial bacterial agent further includes phosphate solubilizing microorganism, and the phosphate solubilizing microorganism is bacillus megaterium Bacillus
Megaterium (CICC20665 is purchased from Chinese industrial Microbiological Culture Collection administrative center), pseudomonad Pseudomonas
Sp. (CGMCC10242 is purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center) or agrobacterium
One of Agrobacterium sp. (CICC10214 is purchased from Chinese industrial Microbiological Culture Collection administrative center) or more
Kind；
In use, domestication of the palpus Jing Guo condition corresponding with area's main environmental conditions will be used, the condition of domestication are as follows: will
Phosphorus decomposing bacterial strain is inoculated into Phos culture medium respectively, and strain passage culture, Phos culture are carried out at 20-25 DEG C of room temperature environment
Based formulas are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/L, KCl 0.5g/L,
FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of sterilizing 20min of 5g/L, pH.
The weight of the phosphate solubilizing microorganism accounts for the 0-20% of microbial bacterial agent.
A kind of preparation method of with improving beach microorganism formulation of plant growth of the present invention, including walk as follows
Suddenly, starch, microbial bacterial agent, algal polysaccharides and biodegradable foamed dose are put into blender in proportion to mix, is stirred
The revolving speed for mixing machine is set as 100-300r/min, and temperature is room temperature, and with improving beach plant is made in reaction time 10-30min
The microorganism formulation of growth.
A kind of microorganism formulation of with improving beach plant growth of the present invention is for improving beach plant growth
Purposes, it is described improve beach plant growth microorganism formulation be used alone or agroforestry organic fertilizer use, compound
Agricultural organic fertilizer is in use, the microorganism formulation of with improving beach plant growth and the quality of agricultural organic fertilizer are 2-4:98-96.
The Brevibacterium sp.TS22 sequence information:
CCCCGCCATCGGCTTCGTTCGAATGCCATGCATAGCGACTCGCACGCCAAGCGTGCGTCCCTGAGCCAT
GATCAAACTCTATGAGTAACACGTGGGCAACCTGCCTATAAGACTGGGATAACTTCGGGAAACCGGAGCTAAT AC
CGGATACGTTCTTTTCTCGCATGAGAGAAGATGGAAAGACGGTTTACGCTGTCACTTATAGATGGGCCCGC GGCG
CATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATC GGCCACAC
TGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGAC GAAAGTCTGACG
GAGCAACGCCGCGTGAACGAAGAAGGCCTTCGGGTCGTAAAGTTCTGTTGTTAGGGAAG AACAAGTACCAGAGTA
ACTGCTGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGC AGCCGCGGTAATACGTAGG
TGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTCCTT AAGTCTGATGTGAAAGCCCACG
GCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGA GGAAAGTGGAATTCCAAAGTGTAGCG
GTGAAATGCGTAGAGATTTGGAGGAACACCAGTGGCGAAGGCGA CTTTCTGGTCTGTAACTGACACTGAGGCGCG
AAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCC ACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGG
TTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACT CCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAA
AGGAATTGACGGGGGCCCGCACAAGCGGTGGAGC ATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGT
CTTGACATCCTCTGACAACCCTAGAGATAGGG CTTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGT
CGTCAGCTCGTGTCGTGAGATGTTGGGTT AAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCA
GTTGGGCACTCTAAGGTGACTGCCGGT GACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTA
TGACCTGGGCTACACACGTGCTAC AATGGATGGTACAAAGGGCTGCAAACCTGCGAAGGTAAGCGAATCCCATAA
AGCCATTCTCAGTTCGGATTG CAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCA
TGCCGCGGTGAATACGTTC CCGGGCCTTGTACACACCGCC
The invention has the benefit that
The improvement beach the microorganism formulation of plant growth core component be derived from Qinghai-Tibet Platean brevibacterium pass through
Low altitude area and room temperature tame and obtain strain, then compound taming again by condition corresponding with that will use area's main environmental conditions
Obtained by phosphate solubilizing bacteria and relevant auxiliary materials, with saline-alkali tolerant, lower oxygen concentration resistance and with improving beach plant growth it is multiple persistently compound function
Effect.The present invention has highly important theory and practice meaning.
Specific embodiment
The following is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as
Protection scope of the present invention.
Embodiment 1
Preparation raw material: 70 parts of starch, 10 parts of microbial bacterial agent, 18 parts of algal polysaccharides, 2 parts of foaming agent.Wherein microbial bacteria
Agent is the bacillus (the two 9:1) that the brevibacterium derived from Qinghai-Tibet Platean obtains by taming again, phosphate solubilizing microorganism is domestication.
Material is put into blender to mix, the revolving speed of blender is set as 180r/min, and temperature is room temperature, reaction
Time is 22min, and the microorganism formulation of with improving beach plant growth is made.
Agricultural organic fertilizer is uniformly mixed when application by 1:99.
Embodiment 2
Preparation raw material: 60 parts of starch, 8 parts of microbial bacterial agent, 28 parts of algal polysaccharides, 4 parts of foaming agent.Wherein microbial bacterial agent
It is obtained to be tamed again derived from the brevibacterium of Qinghai-Tibet Platean process, the agrobacterium (the two 8:2) that phosphate solubilizing microorganism is domestication.
Material is put into blender to mix, the revolving speed of blender is set as 200r/min, and temperature is room temperature, reaction
Time is 20min, and the microorganism formulation of with improving beach plant growth is made.
Agricultural organic fertilizer is uniformly mixed when application by 2:98.
Embodiment 3
Preparation raw material: 68 parts of starch, 8 parts of microbial bacterial agent, 21 parts of algal polysaccharides, 3 parts of foaming agent.Wherein microbial bacterial agent
It is obtained for the unicellular bacterium of vacation derived from Qinghai-Tibet Platean by taming again, the bacillus (the two 8:2) that phosphate solubilizing microorganism is domestication.
Material is put into blender to mix, the revolving speed of blender is set as 100r/min, and temperature is room temperature, reaction
Time is 30min, and the microorganism formulation of with improving beach plant growth is made.
Embodiment 4
Preparation raw material: 65 parts of starch, 5 parts of microbial bacterial agent, 28 parts of algal polysaccharides, 2 parts of foaming agent.Wherein microbial bacterial agent
It is obtained to be tamed again derived from the brevibacterium of Qinghai-Tibet Platean process, the pseudomonad (the two 9:1) that phosphate solubilizing microorganism is domestication.
Material is put into blender to mix, the revolving speed of blender is set as 250r/min, and temperature is room temperature, reaction
Time is 15min, and the microorganism formulation of with improving beach plant growth is made.
Embodiment 5
Preparation raw material: 63 parts of starch, 9 parts of microbial bacterial agent, 25 parts of algal polysaccharides, 3 parts of foaming agent.Wherein microbial bacterial agent
For derived from Qinghai-Tibet Platean by brevibacterium obtained by taming again.
Material is put into blender to mix, the revolving speed of blender is set as 280r/min, and temperature is room temperature, reaction
Time is 12min, and the microorganism formulation of with improving beach plant growth is made.
Embodiment effect
Field trial is to fertilizer functional verification:
Summer in choosing the more consistent experimental plot of condition (relative low temperature low-oxygen environment) in Yancheng, Jiangsu Province, is divided into 18
1 meter × 1 meter block tests each embodiment difference in plot by above-mentioned 5 embodiments and each three design iterations of blank control
It is uniformly blended into soil using identical deal (i.e. 100 grams), sows each 100, green vegetables seed, normal watering Irrigation counts after 10 days
Thinning after emergence rate, then 10 days respectively stays 50 plants；Harvest randomly chooses each 20 plants of titles gross weight after 30 days again.Statistical result such as the following table 1
It is described.
1 embodiment effect statistical form of table
From above-mentioned table 1 as can be seen that using embodiment 1-5 effect relative to blank control group want it is excellent very much.
The emergence rate of plant improves 30% or more, and the biomass of plant improves 30% or more, this explanation can under formula of the invention
Reach with improving beach plant growth.In addition, from 1 result of table it has also been discovered that being properly added agricultural organic fertilizer can significantly improve
Plant growth-promoting effect.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Sequence table
<110>Jiangsu University
<120>a kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1384
<212> DNA
<213>brevibacterium (Brevibacterium sp.)
<400> 1
ccccgccatc ggcttcgttc gaatgccatg catagcgact cgcacgccaa gcgtgcgtcc 60
ctgagccatg atcaaactct atgagtaaca cgtgggcaac ctgcctataa gactgggata 120
acttcgggaa accggagcta ataccggata cgttcttttc tcgcatgaga gaagatggaa 180
agacggttta cgctgtcact tatagatggg cccgcggcgc attagctagt tggtgaggta 240
atggctcacc aaggcgacga tgcgtagccg acctgagagg gtgatcggcc acactgggac 300
tgagacacgg cccagactcc tacgggaggc agcagtaggg aatcttccgc aatggacgaa 360
agtctgacgg agcaacgccg cgtgaacgaa gaaggccttc gggtcgtaaa gttctgttgt 420
tagggaagaa caagtaccag agtaactgct ggtaccttga cggtacctaa ccagaaagcc 480
acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggaatt 540
attgggcgta aagcgcgcgc aggtggttcc ttaagtctga tgtgaaagcc cacggctcaa 600
ccgtggaggg tcattggaaa ctggggaact tgagtgcaga agaggaaagt ggaattccaa 660
agtgtagcgg tgaaatgcgt agagatttgg aggaacacca gtggcgaagg cgactttctg 720
gtctgtaact gacactgagg cgcgaaagcg tggggagcaa acaggattag ataccctggt 780
agtccacgcc gtaaacgatg agtgctaagt gttagagggt ttccgccctt tagtgctgca 840
gctaacgcat taagcactcc gcctggggag tacggccgca aggctgaaac tcaaaggaat 900
tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 960
ttaccaggtc ttgacatcct ctgacaaccc tagagatagg gctttcccct tcgggggaca 1020
gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080
gcaacgagcg caacccttga tcttagttgc cagcattcag ttgggcactc taaggtgact 1140
gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200
gggctacaca cgtgctacaa tggatggtac aaagggctgc aaacctgcga aggtaagcga 1260
atcccataaa gccattctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga 1320
atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380
cgcc 1384
Claims (7)
1. a kind of microorganism formulation of with improving beach plant growth, which is characterized in that including starch, microbial bacterial agent, seaweed
Polysaccharide and biodegradable foamed dose, according to parts by weight, 60-70 parts of starch, 8-10 parts of microbial bacterial agent, algal polysaccharides 18-
28 parts, biodegradable foamed dose 2-5 parts；
The microbial bacterial agent be derived from Qinghai-Tibet Platean brevibacterium Brevibacterium sp.TS22 by low altitude area and often
Docilityization and obtain, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is
CGMCC16576。
2. a kind of microorganism formulation of with improving beach plant growth as described in claim 1, which is characterized in that the low sea
Pull out the condition with room temperature domestication are as follows: the TS22 strain inoculated of Qinghai-Tibetan plateau soil will be isolated from into LB culture medium, in room temperature 20-
Secondary culture, LB culture medium prescription are as follows repeatedly under the conditions of 25 DEG C: tryptone 10g/L, yeast extract 5g/L, sodium chloride
10g/L adjusts the medium pH with NaOH, reaches 7.4, is settled to 1L, high pressure steam sterilization 20min with deionized water；
In use, taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed must be passed through are as follows: will
The TS22 strain inoculated tamed by low altitude area and room temperature is into LB culture medium, the passage training repeatedly under the conditions of 20-25 DEG C of room temperature
It supports, LB culture medium prescription is as follows: tryptone (10g/L), yeast extract (5g/L), sodium chloride (10g/L) are adjusted with NaOH
The medium pH, reaches 7.4, is settled to 1L, high pressure steam sterilization 20min with deionized water.
3. a kind of microorganism formulation of with improving beach plant growth as described in claim 1, which is characterized in that micro- life
Object microbial inoculum further includes phosphate solubilizing microorganism, and the phosphate solubilizing microorganism is one in bacillus megaterium, pseudomonad or agrobacterium
Kind is a variety of；
In use, domestication of the palpus Jing Guo condition corresponding with area's main environmental conditions will be used, the condition of domestication are as follows: by phosphorus decomposing
Bacterial strain is inoculated into Phos culture medium respectively, and strain passage culture is carried out at 20-25 DEG C of room temperature environment, and Phos culture medium is matched
Side are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/L, KCl 0.5g/L,
FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of sterilizing 20min of 5g/L, pH.
4. a kind of microorganism formulation of with improving beach plant growth as claimed in claim 3, which is characterized in that the phosphorus decomposing
The weight of microorganism accounts for the 0-20% of microbial bacterial agent.
5. a kind of preparation method of with improving beach microorganism formulation of plant growth as described in claim 1, feature exist
In including the following steps: that starch, microbial bacterial agent, algal polysaccharides and biodegradable foamed dose are put into stirring in proportion
Machine is mixed, and the revolving speed of blender is set as 100-300r/min, and temperature is room temperature, and reaction time 10-30min is made
With the improving beach microorganism formulation of plant growth.
6. a kind of microorganism formulation of with improving beach plant growth as described in claim 1 is raw for improving beach plant
Long purposes, which is characterized in that the microorganism formulation of with the improving beach plant growth is used alone or agroforestry is organic
Fertilizer uses.
7. purposes as claimed in claim 6, which is characterized in that the microorganism formulation of with the improving beach plant growth is compound
Agricultural organic fertilizer is in use, the microorganism formulation of with improving beach plant growth and the quality of agricultural organic fertilizer are 2-4:98-96.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910074222.0A CN109735465A (en) | 2019-01-25 | 2019-01-25 | A kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910074222.0A CN109735465A (en) | 2019-01-25 | 2019-01-25 | A kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109735465A true CN109735465A (en) | 2019-05-10 |
Family
ID=66366251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910074222.0A Pending CN109735465A (en) | 2019-01-25 | 2019-01-25 | A kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109735465A (en) |
Citations (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007087815A2 (en) * | 2004-12-17 | 2007-08-09 | Metanomics Gmbh | Process for the control of production of fine chemicals |
| EP2287322A1 (en) * | 2002-02-26 | 2011-02-23 | Syngenta Limited | A method of selectively producing male or female sterile plants |
| CN102491853A (en) * | 2011-11-18 | 2012-06-13 | 郑州永丰生物肥业有限公司 | High-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent |
| WO2012175474A1 (en) * | 2011-06-20 | 2012-12-27 | Syngenta Participations Ag | 1,2,3 triazole pesticides |
| CN103146632A (en) * | 2013-02-06 | 2013-06-12 | 山东省果树研究所 | Method for screening multifunctional bacteria for degrading organophosphorus pesticides |
| WO2013188858A2 (en) * | 2012-06-15 | 2013-12-19 | Microvi Biotech Inc. | Novel biocatalyst compositions and processes for use |
| EP2821493A1 (en) * | 2007-05-22 | 2015-01-07 | BASF Plant Science GmbH | Plants with increased tolerance and/or resistance to environmental stress and increased biomass production |
| CN104609995A (en) * | 2015-01-22 | 2015-05-13 | 河海大学 | Plant growth promoting bio-organic fertilizer for saline-alkali land |
| CN105265792A (en) * | 2015-10-27 | 2016-01-27 | 盐城工学院 | Alga fermentation feed additive and preparation method of alga fermentation feed additive |
| CN105272406A (en) * | 2015-10-26 | 2016-01-27 | 北京鑫鑫盛达生物工程技术有限公司 | Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer |
| CN105349436A (en) * | 2015-12-05 | 2016-02-24 | 刘晓红 | Preparation method of wild tricholoma matsutake domesticated mother strains |
| CN105367335A (en) * | 2015-11-19 | 2016-03-02 | 中国科学院南京土壤研究所 | Biochar-based coast saline-alkali land improvement conditioner and preparation method and application thereof |
| CN105884415A (en) * | 2016-04-21 | 2016-08-24 | 福建三炬生物科技股份有限公司 | Compound alga oligosaccharide microbial fertilizer and preparation method thereof |
| CN106279453A (en) * | 2015-05-28 | 2017-01-04 | 易媛 | Macromolecular compound of crosslinking and preparation method thereof, hydrogel, aqueous fracturing fluid and purposes |
| CN106431675A (en) * | 2016-10-08 | 2017-02-22 | 海南博士威农用化学有限公司 | Biological bacterial fertilizer containing bacillus subtilis and application |
| CN108017445A (en) * | 2017-12-05 | 2018-05-11 | 菏泽金正大生态工程有限公司 | A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof |
| CN108040786A (en) * | 2017-12-19 | 2018-05-18 | 重庆市合川区农产品质量安全监督检验检测站 | A kind of implantation methods and interplanting method of salt-soda soil hot pickled mustard tuber |
| CN109134114A (en) * | 2018-11-15 | 2019-01-04 | 合肥华盖生物科技有限公司 | A kind of preparation method of organic biofertilizer |
| CN109730088A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof |
| CN109735466A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof |
| CN109757520A (en) * | 2019-01-25 | 2019-05-17 | 江苏大学 | A kind of microorganism formulation and preparation method thereof promoting Limestone Region plant growth |
| CN110078566A (en) * | 2019-06-04 | 2019-08-02 | 北京中农煦丰生态科技有限公司 | A kind of modifying agent and preparation method thereof for soil of for beach saline-alkali planting trees |
| US20200054021A1 (en) * | 2017-02-22 | 2020-02-20 | Microgen Biotech | A process of constructing specific functional microbiomes for promoting plant growth, plant and soil health, biocontrol and bioremediation |
| US20210076682A1 (en) * | 2017-08-30 | 2021-03-18 | Aobiome Llc | Ammonia oxidizing microorganisms for use in pest control |
-
2019
- 2019-01-25 CN CN201910074222.0A patent/CN109735465A/en active Pending
Patent Citations (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2287322A1 (en) * | 2002-02-26 | 2011-02-23 | Syngenta Limited | A method of selectively producing male or female sterile plants |
| WO2007087815A2 (en) * | 2004-12-17 | 2007-08-09 | Metanomics Gmbh | Process for the control of production of fine chemicals |
| EP2821493A1 (en) * | 2007-05-22 | 2015-01-07 | BASF Plant Science GmbH | Plants with increased tolerance and/or resistance to environmental stress and increased biomass production |
| WO2012175474A1 (en) * | 2011-06-20 | 2012-12-27 | Syngenta Participations Ag | 1,2,3 triazole pesticides |
| CN102491853A (en) * | 2011-11-18 | 2012-06-13 | 郑州永丰生物肥业有限公司 | High-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent |
| WO2013188858A2 (en) * | 2012-06-15 | 2013-12-19 | Microvi Biotech Inc. | Novel biocatalyst compositions and processes for use |
| CN103146632A (en) * | 2013-02-06 | 2013-06-12 | 山东省果树研究所 | Method for screening multifunctional bacteria for degrading organophosphorus pesticides |
| CN104609995A (en) * | 2015-01-22 | 2015-05-13 | 河海大学 | Plant growth promoting bio-organic fertilizer for saline-alkali land |
| CN106279453A (en) * | 2015-05-28 | 2017-01-04 | 易媛 | Macromolecular compound of crosslinking and preparation method thereof, hydrogel, aqueous fracturing fluid and purposes |
| CN105272406A (en) * | 2015-10-26 | 2016-01-27 | 北京鑫鑫盛达生物工程技术有限公司 | Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer |
| CN105265792A (en) * | 2015-10-27 | 2016-01-27 | 盐城工学院 | Alga fermentation feed additive and preparation method of alga fermentation feed additive |
| CN105367335A (en) * | 2015-11-19 | 2016-03-02 | 中国科学院南京土壤研究所 | Biochar-based coast saline-alkali land improvement conditioner and preparation method and application thereof |
| CN105349436A (en) * | 2015-12-05 | 2016-02-24 | 刘晓红 | Preparation method of wild tricholoma matsutake domesticated mother strains |
| CN105884415A (en) * | 2016-04-21 | 2016-08-24 | 福建三炬生物科技股份有限公司 | Compound alga oligosaccharide microbial fertilizer and preparation method thereof |
| CN106431675A (en) * | 2016-10-08 | 2017-02-22 | 海南博士威农用化学有限公司 | Biological bacterial fertilizer containing bacillus subtilis and application |
| US20200054021A1 (en) * | 2017-02-22 | 2020-02-20 | Microgen Biotech | A process of constructing specific functional microbiomes for promoting plant growth, plant and soil health, biocontrol and bioremediation |
| US20210076682A1 (en) * | 2017-08-30 | 2021-03-18 | Aobiome Llc | Ammonia oxidizing microorganisms for use in pest control |
| CN108017445A (en) * | 2017-12-05 | 2018-05-11 | 菏泽金正大生态工程有限公司 | A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof |
| CN108040786A (en) * | 2017-12-19 | 2018-05-18 | 重庆市合川区农产品质量安全监督检验检测站 | A kind of implantation methods and interplanting method of salt-soda soil hot pickled mustard tuber |
| CN109134114A (en) * | 2018-11-15 | 2019-01-04 | 合肥华盖生物科技有限公司 | A kind of preparation method of organic biofertilizer |
| CN109730088A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof |
| CN109735466A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of promotion low temperature, microorganism formulation of low-oxygen environment plant growth and preparation method thereof |
| CN109757520A (en) * | 2019-01-25 | 2019-05-17 | 江苏大学 | A kind of microorganism formulation and preparation method thereof promoting Limestone Region plant growth |
| CN110078566A (en) * | 2019-06-04 | 2019-08-02 | 北京中农煦丰生态科技有限公司 | A kind of modifying agent and preparation method thereof for soil of for beach saline-alkali planting trees |
Non-Patent Citations (7)
| Title |
|---|
| W.H. NOORDMAN 等: "Growth stimulation of Brevibacterium sp. by siderophores", 《JOURNAL OF APPLIED MICROBIOLOGY》 * |
| WANG YING 等: "The diversity and potential function of endophytic bacteria isolated from Kobreasia capilifolia at alpine grasslands on the Tibetan Plateau,China", 《JOUMAT OF INTEGRATIVE AGRICULTURE》 * |
| 何敏 等: "两株耐寒PGPB 的分离及其对藏北当地牧草生长的影响", 《广西植物》 * |
| 夏娟娟: "植物促生内生细菌的筛选及其强化油菜富集土壤铅镐重金属的研究", 《中国优秀硕士学位论文全文数据库（电子期刊）》 * |
| 张强等: "生物刺激素及其在功能水溶性肥料中应用前景分析", 《农业资源与环境学报》 * |
| 李兆坤等: "甲胺磷农药的微生物降解研究进展", 《山东省农业管理干部学院学报》 * |
| 陈秀莉等: "微生物絮凝剂的发展趋势及应用研究", 《阴山学刊(自然科学版)》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101775293B (en) | Biological soil conditioner containing phosphogypsum and application thereof | |
| CN102344812B (en) | Microbiological preparation for improving alkaline land, its preparation method and its application | |
| CN102660486B (en) | Geobacillus sp. UTM01 and application thereof | |
| CN104609995A (en) | Plant growth promoting bio-organic fertilizer for saline-alkali land | |
| CN104911129B (en) | The Bacillus amyloliquefaciens strain and its microbial organic fertilizer of control of plant bacterial wilt | |
| CN105964680B (en) | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof | |
| CN105255782A (en) | Cellulosimicrobium cellulans with reducing capacity on hexavalent chromium and application | |
| CN103952349A (en) | Bacillus aryabhattai, microbial agent and applications of bacillus aryabhattai and microbial agent | |
| CN101717315A (en) | Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof | |
| CN101805716B (en) | Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof | |
| CN104726378B (en) | The method for improving salt stress turfgrass defence enzyme activity using Salt-tolerant microbial agent is strengthened | |
| CN109913375A (en) | A kind of Aspergillus flavus of not toxin producing, the composition containing it and its application | |
| CN109730088A (en) | A kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof | |
| CN102703363A (en) | Bacillus methylotrophicus UTM401 and applications thereof | |
| CN105505843B (en) | One plant of Photosynthetic bacterium strain, the Liquid Fertilizer containing the bacterial strain and preparation method, application | |
| CN109320355A (en) | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application | |
| CN102827797B (en) | Antagonistic bacteria S20 for controlling peanut bacterial wilt, organic microbiological fertilizer prepared thereby, and application | |
| CN102071163A (en) | Multifunctional engineering strain for degrading chlorpyrifos and construction method thereof | |
| CN104789494B (en) | The method for improving turf salt-resistance using garbage compost microbial bacterial agent is strengthened | |
| CN109182194B (en) | Rhizobium oridonii for promoting growth of corolla dentiger and culture method and application thereof | |
| CN109553478A (en) | A kind of biological organic fertilizer and preparation method thereof using the rape stalk exploitation dedicated biological and ecological methods to prevent plant disease, pests, and erosion function of rape | |
| CN105296392A (en) | Fermentation method of bacillus amyloliquefaciens Bam22 for preventing and treating cruciferae plasmodiophora brassicae | |
| CN110951625B (en) | Penicillium phosphate solubilizing PSF and application thereof | |
| CN104774788B (en) | Lawn salt tolerant strengthens the preparation method and application of complex microbial community in garbage compost | |
| CN114874953B (en) | Peanut rhizosphere biocontrol bacterium-Bacillus beleisi SW-1 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190510 |