CN109644616A - A kind of fast breeding method of limacia sagittata seedlings - Google Patents
A kind of fast breeding method of limacia sagittata seedlings Download PDFInfo
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- CN109644616A CN109644616A CN201910066033.9A CN201910066033A CN109644616A CN 109644616 A CN109644616 A CN 109644616A CN 201910066033 A CN201910066033 A CN 201910066033A CN 109644616 A CN109644616 A CN 109644616A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention discloses a kind of fast breeding method of limacia sagittata seedlings, it is related to limacia sagittata field of planting, incubation step includes: to manage after seed selection, disinfection and HORMONE TREATMENT, vernalization, culture substrate processing, smooth arable land, the processing of vernalization root block, nursery, nursery.The present invention is using the limacia sagittata stem tuber of health as kind of a block, by sterilization processing twice, bacterium in limacia sagittata stem tuber can first be killed, it avoids infecting during culture, survival rate is increased to a certain extent, in addition, limacia sagittata stem tuber is handled using the 6-BA basic element of cell division, ABP root-inducing powder, promote the sprouting of limacia sagittata Tuber dormancy bud or adventitious bud, it is promoted to take root simultaneously, germination percentage and survival rate are further increased, and limacia sagittata stem tuber advance germination can be promoted using HORMONE TREATMENT, thereby reduces the period that limacia sagittata seed seedling grows into medicinal material.
Description
Technical field
The present invention relates to limacia sagittata planting technology field more particularly to a kind of fast breeding methods of limacia sagittata seedlings.
Background technique
Limacia sagittata Tinospora sagittata (Oliv.) Gagnep. is Menispermaceae liana, and main product is in Sichuan, lake
The provinces and regions such as south, Guangxi, Hubei, Guizhou, dried root are the sources of Chinese medicine tinosporae.Tinosporae is going through version " Chinese Pharmacopoeia "
In record, have it is clearing heat and detoxicating, relieving sore-throat, analgesic the effect of, cure mainly abscess of throat, ulcer serious case of furuncle, diarrhea, dysentery, stomach duct and abdomen
Hot pain etc. is the precious Chinese herbal medicine in Southwestern China area.Modern pharmacology research shows that tinosporae has apparent anti-inflammatory and antalgic, resists
The effects of bacterium, antitumor, hypoglycemic, resisting stress and removing toxic substances, and it is called the natural drug of alternative antibiotic, because it is disease-resistant
The special efficacy of poison, anti-tumor aspect, is devoted to always new drug development research for many years, and demand increases year by year.
And limacia sagittata is during growing naturally, grows into period of medicinal material up to 4 years or more from seed seedling, knot of blooming
The real time is 3 years or so, and all the time, the acquisition of limacia sagittata, which depends on, to be taken wild product and make medicinal, and artificial cultivation is not also real
The development of matter, is not planted due to only digging, and growth it is slower, wild resource is increasingly exhausted, artificial growth and foster it is imperative.
Currently, the breeding of limacia sagittata mainly passes through stem tuber division propagation, cuttage and seedling culture, based on seminal propagation, due to limacia sagittata male and female
Different strain, pollination are influenced greatly by environment, and Natural seed setting rate is low, resource investigation with introduce a fine variety middle discovery, staminiferous plant far more than female plant (about
50: 1), leading to that limacia sagittata setting percentage is very low, seed amount is few, and seed has apparent dormant period, germination percentage is lower, limacia sagittata
Cutting propagation survival rate is also very low, and limacia sagittata division propagation is also faced with always that germination is slow, germination percentage is low, survival rate is low asks
Topic.
Summary of the invention
In view of the above-mentioned problems, a kind of fast breeding method of limacia sagittata seedlings of the present invention, is kind of a block with limacia sagittata stem block, it is right
It carries out vernalization and twice disinfection, HORMONE TREATMENT, increases the germination percentage and survival rate of limacia sagittata stem block, and reduce black ox
Gallbladder seed seedling grows into the period of medicinal material.
The present invention solves above-mentioned technical problem by following technological means:
A kind of fast breeding method of limacia sagittata seedlings, comprising the following steps:
Seed selection: in annual September, health is chosen, the limacia sagittata stem tuber of no disease and pests harm is planted；
Disinfection and HORMONE TREATMENT: the limacia sagittata stem tuber chosen is become into single root block from cutting from connection gap bridge, with more bacterium
Clever solution takes out after impregnating 2-3 minutes to be dried, then is impregnated 2 hours with the 6-BA basic element of cell division of 2mg/L, and taking-up is dried, and is obtained
Sterilized root block, for use；
Vernalization: sterilized root block is placed in germination accelerating pond, in 20-25 DEG C heat preservation 15-20 days, obtain vernalization root block；
Culture substrate processing: configured culture substrate is sprayed with carbendazim solution, so that water content is reached 60%, film
Sterilization after covering 3-4 days, for use；
Smooth arable land: after carrying out smooth, weeding to arable land, sunshade net, the screening of the sunshade net are built in the overhead in arable land
Light rate is 68-72%；
The processing of vernalization root block: it takes out and dries after vernalization root block carbendazim solution is impregnated 2-3 minutes, reuse ABP
Root-inducing powder impregnates 1 hour, takes out, dries for use；
Nursery: by treated, culture medium is packed into the seedling-cultivation plate with leakage hole, and packed height 1.5-3cm will locate
Vernalization root block after reason is embedded in seedling-cultivation plate in the way of one plant of a cave, then covers the culture medium of 1-2cm thickness, and seedling-cultivation plate is placed in
In arable land, then in the straw of one layer of 3cm thickness of the top of seedling-cultivation plate covering；
It is managed after nursery: it is primary every watering in 1-3 days after nursery, keep culture substrate water content in 55-65%, and with
When weed out the rank grass, after emergence, added the cooperation sprinkling of 1000 times of Rogor with 800 times of ambam every 10-15 days, cultivated after emergence to working as
Year September part obtains limacia sagittata seedlings.
Further, the maximum gauge of the single limacia sagittata stem tuber is 0.5-2cm.
Further, the step of vernalization specifically, successively spread from the bottom up in the bottom of pond of germination accelerating pond and the inner wall at four sides
If the straw shone well, cavings, 3-4 layer toilet paper, sterilized root block are lain in germination accelerating pond layer by layer, in sterilized root after the completion of stacking
The straw that 10cm thickness is re-layed above block re-lays heat preservation outer layer above straw, and keeping temperature in germination accelerating pond is 20-25
DEG C, 15-20 days are kept the temperature, vernalization root block is obtained.
Further, the stacking number of plies of the sterilized root block is no more than 3 layers, and thickness is no more than 10cm.
Further, the culture substrate is prepared using detritus Nutrition Soil and loose brunisolic soil according to the ratio of 1:3.
Further, the carbendazim solution is 1000 times of carbendazim dilution.Fungicide of the carbendazim as wide spectrum,
It can be by the bacterium killing as much as possible in limacia sagittata stem tuber.
Further, in the smooth arable land step, the height of the sunshade net is 1.5-2.0m.
Further, in the nursery step, the bore of the seedling-cultivation plate is 6cm, and cave depth is 5-7cm.
Further, in the nursery step, multiple seedling-cultivation plates constitute seedling-cultivation plate group, and the seedling-cultivation plate group width is
1.2m, the distance between two neighboring seedling-cultivation plate group are 0.5m.The distance between two neighboring seedling-cultivation plate group is convenient subsequent
The progress of management work.
Beneficial effects of the present invention: the present invention by the limacia sagittata stem tuber of health as kind of a block, by sterilization processing twice,
Bacterium in limacia sagittata stem tuber can first be killed, avoid infecting during culture, increase to a certain extent
Survival rate, in addition, using the 6-BA basic element of cell division, ABP root-inducing powder handle limacia sagittata stem tuber, promote limacia sagittata Tuber dormancy bud or
The sprouting of adventitious bud, while it being promoted to take root, germination percentage and survival rate are further increased, and blueness can be promoted using HORMONE TREATMENT
Ox gallbladder stem tuber advance germination thereby reduces the period that limacia sagittata seed seedling grows into medicinal material.
Specific embodiment
Below with reference to specific embodiment, the present invention is described in detail:
A kind of fast breeding method of limacia sagittata seedlings of the invention, to the limacia sagittata stem block done kind carry out sterilization twice and
HORMONE TREATMENT improves its survival rate and germination percentage.
Embodiment one
Seed selection: choosing health, no disease and pests harm during annual September, single maximum gauge is the black ox of 0.5-2cm
Gallbladder stem tuber is planted；
Disinfection and HORMONE TREATMENT: the limacia sagittata stem tuber chosen is become into single root block from cutting from connection gap bridge, with 1000
Times carbendazim dilution takes out after impregnating 2 minutes to be dried, then with the immersion of the 6-BA basic element of cell division 2 hours of 2mg/L, taking-up was dried in the air
It is dry, sterilized root block is obtained, for use；
Vernalization: building a rectangle germination accelerating pond with sun-dried mud brick indoors, bay wall is 60 centimetres high, bottom of pond and four sides in germination accelerating pond
Inner wall successively lays the straw that 10cm thickness shone, cavings, the 3 layers of toilet paper of 5cm thickness from the bottom up, fine warm weather is selected, by nothing
Mycorhiza block is lain in germination accelerating pond layer by layer, stack the number of plies be 2 layers, with a thickness of 7cm, after the completion of stacking the top of sterilized root block again
It is laid with the straw of 10cm thickness, cotton blanket is re-layed above straw as heat preservation outer layer, keeping temperature in germination accelerating pond is 20-25
DEG C, 20 days are kept the temperature, vernalization root block is obtained；
Culture substrate processing: by detritus Nutrition Soil with loose brunisolic soil according to the proportional arrangement of 1:3, by configured culture
Matrix is sprayed with 1000 times of carbendazim dilutions, so that water content is reached 60%, after film covers 3 days sterilizations, for use；
Smooth arable land: after carrying out smooth, weeding to arable land, the sunshade net of 1.5m high, sunshade net are built in the overhead in arable land
Shading rate be 68%；
The processing of vernalization root block: it takes out and dries after vernalization root block is impregnated 3 minutes with 1000 times of carbendazim dilutions, then make
It is impregnated 1 hour with the ABP root-inducing powder of 1000 times of liquid, takes out, dries for use；
Nursery: by treated, culture medium is packed into the seedling-cultivation plate with leakage hole, and the bore of seedling-cultivation plate is 6cm, and cave is deep
For 5cm, packed height 2cm, treated vernalization root block is embedded in seedling-cultivation plate in the way of one plant of a cave, then cover 1cm
Thick culture substrate, seedling-cultivation plate is placed in arable land, and multiple seedling-cultivation plates constitute seedling-cultivation plate group, and seedling-cultivation plate group width is 1.2m, phase
The distance between adjacent two seedling-cultivation plate groups are 0.5m, finally cover the straw of one layer of 3cm thickness in the top of seedling-cultivation plate again；
It is managed after nursery: it is primary every watering in 3 days after nursery, keep culture substrate water content in 55-65%, and at any time
It weeds out the rank grass, after emergence, added 1000 times of Rogor cooperation sprinklings with 800 times of ambam every 10 days, cultivate after emergence to current year September
Part obtains limacia sagittata seedlings.
Wherein, in vernalization step, the length and width of germination accelerating pond is depending on the number of limacia sagittata stem block.
Embodiment two
Seed selection: choosing health, no disease and pests harm during annual September, single maximum gauge is the black ox of 0.5-2cm
Gallbladder stem tuber is planted；
Disinfection and HORMONE TREATMENT: the limacia sagittata stem tuber chosen is become into single root block from cutting from connection gap bridge, with 1000
Times carbendazim dilution takes out after impregnating 3 minutes to be dried, then with the immersion of the 6-BA basic element of cell division 2 hours of 2mg/L, taking-up
It dries, obtains sterilized root block, for use；
Vernalization: a rectangle germination accelerating pond is built with brick indoors, bay wall is 60 centimetres high, in the bottom of pond of germination accelerating pond and four sides
Wall successively lays the straw that 10cm thickness shone, cavings, the 4 layers of toilet paper of 5cm thickness from the bottom up, selects fine warm weather, will be sterile
Root block is lain in germination accelerating pond layer by layer, stack the number of plies be 3 layers, with a thickness of 9.8cm, after the completion of stacking the top of sterilized root block again
It is laid with the straw of 10cm thickness, cotton-wadded quilt is re-layed above straw as heat preservation outer layer, keeping temperature in germination accelerating pond is 20-25
DEG C, 15 days are kept the temperature, vernalization root block is obtained；
Culture substrate processing: by detritus Nutrition Soil with loose brunisolic soil according to the proportional arrangement of 1:3, by configured culture
Matrix is sprayed with 1000 times of carbendazim dilutions, so that water content is reached 60%, after film covers 4 days sterilizations, for use；
Smooth arable land: after carrying out smooth, weeding to arable land, building the sunshade net of 2m high in the overhead in arable land, sunshade net
Shading rate is 70%；
The processing of vernalization root block: it takes out and dries after vernalization root block is impregnated 2 minutes with 1000 times of carbendazim dilutions, then make
It is impregnated 1 hour with the ABP root-inducing powder of 1000 times of liquid, takes out, dries for use；
Nursery: by treated, culture medium is packed into the seedling-cultivation plate with leakage hole, and the bore of seedling-cultivation plate is 6cm, and cave is deep
For 7cm, packed height 1.5cm, treated vernalization root block is embedded in seedling-cultivation plate in the way of one plant of a cave, then is covered
The culture medium of 2cm thickness, seedling-cultivation plate is placed in arable land, and multiple seedling-cultivation plates constitute seedling-cultivation plate group, and seedling-cultivation plate group width is 1.2m, phase
The distance between adjacent two seedling-cultivation plate groups are 0.5m, finally cover the straw of one layer of 3cm thickness in the top of seedling-cultivation plate again；
It is managed after nursery: it is primary every watering in 1 day after nursery, keep culture substrate water content in 55-65%, and at any time
It weeds out the rank grass, after emergence, added 1000 times of Rogor cooperation sprinklings with 800 times of ambam every 15 days, cultivate after emergence to current year September
Part obtains limacia sagittata seedlings.
Wherein, in vernalization step, the length and width of germination accelerating pond is depending on the number of limacia sagittata stem block.
Embodiment three
Seed selection: choosing health, no disease and pests harm during annual September, single maximum gauge is the black ox of 0.5-2cm
Gallbladder stem tuber is planted；
Disinfection and HORMONE TREATMENT: the limacia sagittata stem tuber chosen is become into single root block from cutting from connection gap bridge, with 1000
Times carbendazim dilution takes out after impregnating 2 minutes to be dried, then with the immersion of the 6-BA basic element of cell division 2 hours of 2mg/L, taking-up
It dries, obtains sterilized root block, for use；
Vernalization: a rectangle germination accelerating pond is built with brick indoors, bay wall is 60 centimetres high, in the bottom of pond of germination accelerating pond and four sides
Wall successively lays the straw that 10cm thickness shone, cavings, the 3 layers of toilet paper of 5cm thickness from the bottom up, selects fine warm weather, will be sterile
Root block is lain in germination accelerating pond layer by layer, and stacking the number of plies is 1 layer, with a thickness of 5cm, is repaved above sterilized root block after the completion of stacking
If the straw of 10cm thickness, cotton blanket is re-layed above straw as heat preservation outer layer, keeping temperature in germination accelerating pond is 20-25 DEG C,
Heat preservation 18 days, obtains vernalization root block；
Culture substrate processing: by detritus Nutrition Soil with loose brunisolic soil according to the proportional arrangement of 1:3, by configured culture
Matrix impregnates sprinkling with 1000 times of carbendazim dilutions, so that water content is reached 60%, after film covers 3 days sterilizations, for use；
Smooth arable land: after carrying out smooth, weeding to arable land, the sunshade net of 1.8m high, sunshade net are built in the overhead in arable land
Shading rate be 72%；
The processing of vernalization root block: taking out after vernalization root block is impregnated 3 minutes with 1000 times of carbendazim dilutions and dry,
The ABP root-inducing powder for reusing 1000 times of liquid impregnates 1 hour, takes out, dries for use；
Nursery: by treated, culture medium is packed into the seedling-cultivation plate with leakage hole, and the bore of seedling-cultivation plate is 6cm, and cave is deep
For 7cm, packed height 3cm, treated vernalization root block is embedded in seedling-cultivation plate in the way of one plant of a cave, then is covered
The culture substrate of 1.5cm thickness, seedling-cultivation plate is placed in arable land, and multiple seedling-cultivation plates constitute seedling-cultivation plate group, and seedling-cultivation plate group width is
1.2m, the distance between two neighboring seedling-cultivation plate group are 0.5m, finally cover the rice of one layer of 3cm thickness in the top of seedling-cultivation plate again
Grass；
It is managed after nursery: it is primary every watering in 2 days after nursery, keep culture substrate water content in 55-65%, and at any time
It weeds out the rank grass, after emergence, added 1000 times of Rogor cooperation sprinklings with 800 times of ambam every 12 days, cultivate after emergence to current year September
Part obtains limacia sagittata seedlings.
Wherein, in vernalization step, the length and width of germination accelerating pond is depending on the number of limacia sagittata stem block.
Detritus Nutrition Soil used in culture substrate processing step is by animal waste, bean dregs, paddy in above-described embodiment
After shell, fallen leaves, soil are crushed, mixed, leavening is added, stirs evenly, is crushed after sealing and fermenting and obtain decomposed soil, will
To decomposed soil mixing plant ash, fish meal, slow-releasing granules obtain final detritus Nutrition Soil used.
Wherein slow-releasing granules are the nucleocapsid knots of compound one layer of chitosan layer outside core using spherical degradable micropore ceramics as core
Structure, chitosan layer are the chitosans modified with agar, have porous structure.Its it is specific the preparation method is as follows:
Take respectively 1g heteroauxin, 2g zeatin, 1g compound sodium nitrophenolate, 2g methyl α-naphthyl acetate, 1g Thidiazuron with a small amount of 95% wine
After smart solution is completely dissolved, be diluted with water to 10000 times respectively, take respectively dilution after heteroauxin solution, zeatin solution,
Compound sodium nitrophenolate solution, naphthalene acid solution, Thidiazuron solution are mixed according to volume ratio 2:1:1:2:1, obtain growth regulating after stirring
Mixed liquor.
15g zinc sulfate, 5g ammonium molybdate, 5g boric acid, 10g manganese sulfate, 5g ferrous sulfate, the mixing of 10g anhydrous cupric sulfate are taken, is added
Enter suitable water, after 20-30min dissolution is stirred under the speed of 200-300r/min, adds water and be diluted to 1000 times, obtain micro-
Secondary element make-up solution.
Degradable micropore ceramics are immersed in microelement make-up solution, are taken out after standing 1-2 days, sustained release ceramics are obtained
Particle, then take modified chitosan that growth regulating mixed liquor is added to be modulated into paste, the amylan of 1% chitosan mass is added, it will
Sustained release ceramic particle taking-up is placed in modification of chitosan, is stirred, so that modification of chitosan is equably combined to sustained release ceramic particle
Outside, dry in shady place after taking-up, obtain slow-releasing granules.
It is micro- when being immersed in microelement make-up solution since degradable micropore ceramics particle is porous structure
Secondary element replenisher can fill in the gap in degradable micropore ceramics particle completely, and modified with the modulation of growth regulating mixed liquor
Chitosan, modified chitosan have porous mechanism, growth regulating mixed liquor can be adsorbed onto inside chitosan；It is inciting somebody to action
After chitosan is combined on sustained release ceramic particle, during use, external chitosan layer is decomposed first, absorbs, shell
The growth hormone adsorbed in glycan layer can break the suspend mode of bud, promote cell division, promote the sprouting of bud, promote limacia sagittata stem
Block is taken root, and is further increasing the survival rate and germination percentage of limacia sagittata stem block to a certain degree, while when external chitosan layer point
Xie Hou, the microelement in internal sustained release ceramic particle start to come out, provide necessary iron in growing process,
The microelements such as molybdenum, zinc, while the ceramic particle being sustained can be avoided the hardened of Nutrition Soil on the one hand, on the other hand using can
The material of degradation is sustained ceramic particle by weathering, degradation during cultivation, will not influence the limacia sagittata stem block of next batch
Cultivation, while the ceramic powders of weathering are equally capable of providing some microelements.
Germination by the experiment of above embodiments, to the limacia sagittata stem block that embodiment 1, embodiment 2, embodiment 3 are cultivated
Rate and survival rate are counted, and following data is obtained:
| Embodiment | Survival rate | Germination percentage |
| Embodiment 1 | 88.9% | 92.3% |
| Embodiment 2 | 84.6% | 91.8% |
| Embodiment 3 | 86.7% | 94.1% |
By above data it is recognised that the limacia sagittata seedlings cultivated using breeding method of the invention, germination percentage are mentioned
Height is to 90% or more, while survival rate is also 80% or more.
The above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to preferred embodiment to this hair
It is bright to be described in detail, those skilled in the art should understand that, it can modify to technical solution of the present invention
Or equivalent replacement should all cover without departing from the objective and range of technical solution of the present invention in claim of the invention
In range.Technology not described in detail in the present invention, shape, construction portion are well-known technique.
Claims (9)
1. a kind of fast breeding method of limacia sagittata seedlings, which comprises the following steps:
Seed selection: in annual September, health is chosen, the limacia sagittata stem tuber of no disease and pests harm is planted；
Disinfection and HORMONE TREATMENT: becoming single root block from cutting from connection gap bridge for the limacia sagittata stem tuber chosen, molten with carbendazim
Liquid takes out after impregnating 2-3 minutes to be dried, then is impregnated 2 hours with the 6-BA basic element of cell division of 2mg/L, and taking-up is dried, and is obtained sterile
Root block, for use；
Vernalization: sterilized root block is placed in germination accelerating pond, in 20-25 DEG C heat preservation 15-20 days, obtain vernalization root block；
Culture substrate processing: configured culture substrate is sprayed with carbendazim solution, so that water content is reached 60%, film covering
After 3-4 days sterilizations, for use；
Smooth arable land: after carrying out smooth, weeding to arable land, sunshade net, the shading rate of the sunshade net are built in the overhead in arable land
For 68-72%；
The processing of vernalization root block: it takes out and dries after vernalization root block carbendazim solution is impregnated 2-3 minutes, reuse ABP and take root
Powder impregnates 1 hour, takes out, dries for use；
Nursery: by treated, culture medium is packed into the seedling-cultivation plate with leakage hole, packed height 1.5-3cm, after processing
Vernalization root block be embedded in seedling-cultivation plate in the way of one plant of a cave, then cover the culture medium of 1-2cm thickness, seedling-cultivation plate be placed in arable land
It is interior, then the straw of one layer of 3cm thickness is covered in the top of seedling-cultivation plate；
It is managed after nursery: it is primary every watering in 1-3 days after nursery, keep culture substrate water content to pull out in 55-65%, and at any time
It cuts weeds, after emergence, added 1000 times of Rogor cooperation sprinklings with 800 times of ambam every 10-15 days, cultivated after emergence to current year 9
Obtain limacia sagittata seedlings month.
2. a kind of fast breeding method of limacia sagittata seedlings according to claim 1, which is characterized in that the single black ox
The maximum gauge of gallbladder stem tuber is 0.5-2cm.
3. a kind of fast breeding method of limacia sagittata seedlings according to claim 2, which is characterized in that the step of the vernalization
Suddenly specifically, successively laying straw, cavings, 3-4 layer grass shone from the bottom up in the bottom of pond of germination accelerating pond and the inner wall at four sides
Paper lies in sterilized root block in germination accelerating pond layer by layer, re-lays the straw of 10cm thickness after the completion of stacking above sterilized root block,
Heat preservation outer layer is re-layed above straw, keeping temperature in germination accelerating pond is 20-25 DEG C, keeps the temperature 15-20 days, obtains vernalization root
Block.
4. a kind of fast breeding method of limacia sagittata seedlings according to claim 3, which is characterized in that the sterilized root block
The stacking number of plies be no more than 3 layers, thickness be no more than 10cm.
5. a kind of fast breeding method of limacia sagittata seedlings according to claim 4, which is characterized in that the culture substrate
It is prepared using detritus Nutrition Soil and loose brunisolic soil according to the ratio of 1:3.
6. a kind of fast breeding method of limacia sagittata seedlings according to claim 5, which is characterized in that the carbendazim is molten
Liquid is 1000 times of carbendazim dilution.
7. a kind of fast breeding method of limacia sagittata seedlings according to claim 6, which is characterized in that the smooth arable land
In step, the height of the sunshade net is 1.5-2.0m.
8. a kind of fast breeding method of limacia sagittata seedlings according to claim 7, which is characterized in that the nursery step
In, the bore of the seedling-cultivation plate is 6cm, and cave depth is 5-7cm.
9. a kind of fast breeding method of limacia sagittata seedlings according to claim 8, which is characterized in that the nursery step
In, multiple seedling-cultivation plates constitute seedling-cultivation plate groups, and the seedling-cultivation plate group width is 1.2m, between two neighboring seedling-cultivation plate group away from
From for 0.5m.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713327A (en) * | 2020-06-30 | 2020-09-29 | 四川省中医药科学院 | Planting method for intercropping tinospora root and pear trees |
| CN112400639A (en) * | 2020-11-04 | 2021-02-26 | 黔东南州扶贫宣传教育中心 | Method for improving sprouting of tinospora capillipes seeds |
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| CN111713327B (en) * | 2020-06-30 | 2022-01-25 | 四川省中医药科学院 | Planting method for intercropping tinospora root and pear trees |
| CN112400639A (en) * | 2020-11-04 | 2021-02-26 | 黔东南州扶贫宣传教育中心 | Method for improving sprouting of tinospora capillipes seeds |
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