CN109497108A - A kind of coptis soil-borne disease control agent and preparation method thereof - Google Patents
A kind of coptis soil-borne disease control agent and preparation method thereof Download PDFInfo
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- CN109497108A CN109497108A CN201811586318.7A CN201811586318A CN109497108A CN 109497108 A CN109497108 A CN 109497108A CN 201811586318 A CN201811586318 A CN 201811586318A CN 109497108 A CN109497108 A CN 109497108A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of coptis soil-borne disease control agents and preparation method thereof, belong to soil-borne disease Prevention Technique field.The control agent is made of the raw material components of following parts by weight: 3-5 parts and 10-25 parts of straw extracting solution of bacillus subtilis fermentation liquor: the preparation method the present invention also provides above-mentioned control agent is that autoclave is added in the straw that will be crushed, add 2-3 times of parts by weight of ethylene glycol, heating, heat preservation, collect filtrate, filtrate is distilled, and is stood, is obtained straw extracting solution；Bacillus subtilis fermentation liquor is prepared, bacillus subtilis fermentation liquor and straw extracting solution are sufficiently mixed according to weight part ratio to get the coptis soil-borne disease control agent.Fermentation of bacillus subtilis agent in the present invention, which can secrete, generates antagonism element, soil can be more effectively inhibited to pass the growth of bacterium, infect and spread under the synergistic effect of straw extracting solution, the control efficiency of soil-borne disease powdery mildew and southern blight to coptis most serious is obvious.
Description
Technical field
The present invention relates to soil-borne disease Prevention Technique fields, and in particular to a kind of coptis soil-borne disease control agent and its preparation
Method.
Background technique
Soil-borne disease refers to that the germ in the pathogen or diseased plant residuum of life in the soil encroaches on from root or stem
Disease caused by crop.Soil-borne disease is difficult because having the characteristics that concealment, infectiousness, burst, destructiveness and prevention and treatment
Restraining factors as China's sustainable development of agricultural production.Southern blight and powdery mildew are the soil-borne diseases that most serious occurs for the coptis
Evil, and cause the major reason of coptis continuous cropping obstacle.Currently, the tradition prevention and treatment of stone column coptis soil-borne disease mainly uses medicament
Prevention and treatment, breeding resistant variety, crop rotation etc., although these control methods can reduce the incidence of soil-borne disease to a certain extent,
But simultaneously there is also many drawbacks, if Fallow crop rotation is wherein most economical effectively preventing method, but the rhizome of the coptis 4~5 years
Excavation, soil-borne disease can just be effectively prevented by generalling use 3 years or more Fallow crop rotations, and this method feasibility is low, it is difficult to implement；Its
Secondary selection disease-resistant variety plays a degree of effect to the prevention and treatment of specific single soil-borne disease, however to most of
Soil-borne disease preventive effect is little；For another example connect the chemical reagent such as agriculture multi-purpose hymexazol, Flusilazole, Mancozeb to carry out disinfection to come to soil
The generation of soil-borne disease is controlled, but crops are also easy to produce dependence, and a large amount of uses of pesticide to pesticide, can not only bring a system
The environmental problem of column, and the residual quality and outlet that can seriously affect medicinal material of agriculture.However, these traditional control methods cannot be effective
Solve the problems, such as the generation of soil-borne disease, therefore, searching is highly efficient, and free of contamination coptis soil-borne disease control agent is extremely urgent.
Summary of the invention
The purpose of the invention is to overcome the problems of the prior art, provide a kind of coptis soil-borne disease control agent and its
Preparation method.
The present invention provides a kind of coptis soil-borne disease control agents, are made of the raw material components of following parts by weight: withered grass bud
3-5 parts and 10-25 parts of straw extracting solution of spore bacillus fermentation liquid.
Preferably, being made of the raw material components of following parts by weight: 3-5 parts of bacillus subtilis fermentation liquor and straw extracting solution
10-15 parts.
Preferably, being made of the raw material components of following parts by weight: 5 parts of bacillus subtilis fermentation liquor and straw extracting solution 12
Part.
The present invention also provides the preparation methods of above-mentioned coptis soil-borne disease control agent, comprising the following steps:
Autoclave is added Step 1: preparing after straw is crushed to 2-3cm by straw extracting solution, adds 2-3 times of parts by weight
Ethylene glycol, be heated to 60-80 DEG C, keep the temperature 15-30min, collect filtrate, filtrate is distilled, and is stood, and straw extraction is obtained
Liquid；
Step 2: preparation bacillus subtilis fermentation liquor is packed into NA culture medium in a reservoir, through 115-121 DEG C of high pressure sterilization
20-40min is cooled to room temperature；The Bacillus subtilis strain that inclined-plane is saved, is seeded on NA culture medium, 37 DEG C, 100-
120rpm shaking table shake culture 16-20h, is made seed liquor；The bacillus subtilis seed liquor of preparation is pressed to the ratio of 0.1-0.2%
Example, which is linked into the fermentor for have been loaded with fermentation medium, cultivates 18-25h；Up to bacillus subtilis bacterium solution；
Step 3: by bacillus subtilis fermentation liquor and straw extracting solution according to weight part ratio be sufficiently mixed to get
The coptis soil-borne disease control agent.
Preferably, the ingredient of NA culture medium is peptone 26g/L, beef extract 5g/L and sodium chloride 3g/L, pH 7.5.
Preferably, the ingredient of fermentation medium is potato 40.2g/L, beancake powder 15g/L, glucose 2g/L and sodium chloride
5g/L is adjusted to pH 7.5 before medium sterilization.
Compared with prior art, the beneficial effects of the present invention are: coptis soil-borne disease prevention and treatment agent prescription of the invention is simple,
Low in cost, pollution-free, the control efficiency of soil-borne disease powdery mildew and southern blight to coptis most serious is obvious, the coptis of the present invention
Fermentation of bacillus subtilis agent in soil-borne disease control agent, which can secrete, generates antagonism element, under the synergistic effect of straw extracting solution
The growth of soil-borne disease pathogen can more effectively be inhibited, infect and spread.
Specific embodiment
Below with reference to embodiment, specific embodiments of the present invention will be described in detail, it is to be understood that of the invention
Protection scope is not limited by the specific implementation.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
(Bacillus subtilis) deposit number of bacillus subtilis used in the present embodiment is CGMCC
NO.7850.The concentration of bacillus subtilis fermentation liquor Bacillus is 5 × 1010cfu/mL。
Embodiment 1
A kind of coptis soil-borne disease control agent provided in this embodiment, is made: withered grass of the raw material components of following parts by weight
5 parts and 12 parts of straw extracting solution of fermentation of bacillus liquid；
The preparation method of above-mentioned control agent the following steps are included:
Autoclave is added Step 1: preparing after straw is crushed to 2cm by straw extracting solution, adds 3 times of parts by weight of second
Glycol is heated to 75 DEG C, keeps the temperature 17min, collects filtrate, and filtrate is distilled, and stands, obtains straw extracting solution；
Step 2: preparation bacillus subtilis fermentation liquor is packed into NA culture medium in a reservoir, through 120 DEG C of high pressure sterilizations
30min is cooled to room temperature；The Bacillus subtilis strain that inclined-plane is saved, is seeded on NA culture medium, 37 DEG C, 120rpm shakes
Bed shake culture 18h, is made seed liquor；The bacillus subtilis seed liquor of preparation is linked into 0.1% ratio and is had been loaded with
It is cultivated for 24 hours in the fermentor of fermentation medium；Up to bacillus subtilis bacterium solution；
The ingredient of NA culture medium is peptone 26g/L, beef extract 5g/L and sodium chloride 3g/L, pH 7.5.Fermentation medium
Ingredient be potato 40.2g/L, beancake powder 15g/L, glucose 2g/L and sodium chloride 5g/L, be adjusted to pH before medium sterilization
7.5.Fermentation condition in fermentor are as follows: 37 DEG C of temperature, pH=7, dissolved oxygen concentration maintains 25% saturation degree, 220rpm.
Step 3: by bacillus subtilis fermentation liquor and straw extracting solution according to weight part ratio be sufficiently mixed to get
The coptis soil-borne disease control agent.
Embodiment 2
A kind of coptis soil-borne disease control agent provided in this embodiment, is made withered grass bud by the raw material components of following parts by weight
5 parts and 12 parts of straw extracting solution of spore bacillus fermentation liquid；
The preparation method of control agent the following steps are included:
Autoclave is added Step 1: preparing after straw is crushed to 3cm by straw extracting solution, adds 2.5 times of parts by weight
Ethylene glycol is heated to 80 DEG C, keeps the temperature 30min, collects filtrate, and filtrate is distilled, and stands, obtains straw extracting solution；
Step 2: preparation bacillus subtilis fermentation liquor is packed into NA culture medium in a reservoir, through 115 DEG C of high pressure sterilizations
40min is cooled to room temperature；The Bacillus subtilis strain that inclined-plane is saved, is seeded on NA culture medium, 37 DEG C, 110rpm shakes
Bed shake culture 20h, is made seed liquor；The bacillus subtilis seed liquor of preparation is linked into 0.2% ratio and is had been loaded with
20h is cultivated in the fermentor of fermentation medium；Up to bacillus subtilis bacterium solution；
The ingredient of NA culture medium is peptone 26g/L, beef extract 5g/L and sodium chloride 3g/L, pH are 7.5 ± 0.1.Fermentation
The ingredient of culture medium is potato 40.2g/L, beancake powder 15g/L, glucose 2g/L and sodium chloride 5g/L, is adjusted before medium sterilization
It saves to pH 7.5 ± 0.1.Fermentation condition in fermentor are as follows: 37 DEG C of temperature, pH=7, it is full that dissolved oxygen concentration maintains 30%
And degree, 220rpm.
Step 3: by bacillus subtilis fermentation liquor and straw extracting solution according to weight part ratio be sufficiently mixed to get
The coptis soil-borne disease control agent.
Embodiment 3
A kind of coptis soil-borne disease control agent provided in this embodiment, is made: withered grass of the raw material components of following parts by weight
4 parts and 20 parts of straw extracting solution of fermentation of bacillus liquid；The preparation method of the control agent of the present embodiment is same as Example 1.
Embodiment 4
A kind of coptis soil-borne disease control agent provided in this embodiment, is made: withered grass of the raw material components of following parts by weight
5 parts and 20 parts of straw extracting solution of fermentation of bacillus liquid；The preparation method of the control agent of the present embodiment is same as Example 1.
Comparative example 1
A kind of coptis soil-borne disease control agent provided in this embodiment is bacillus subtilis fermentation liquor 3-5 parts and water 10-
25 parts；The preparation method is as follows:
Preparation bacillus subtilis fermentation liquor is packed into NA culture medium in a reservoir, cooling through 120 DEG C of high pressure sterilization 30min
To room temperature；The Bacillus subtilis strain that inclined-plane is saved, is seeded on NA culture medium, 37 DEG C, 120rpm shaking table shake culture
Seed liquor is made in 18h；The bacillus subtilis seed liquor of preparation is linked into 0.1% ratio and has been loaded with fermentation medium
Fermentor in cultivate for 24 hours；Up to bacillus subtilis bacterium solution；
The ingredient of NA culture medium is peptone 26g/L, beef extract 5g/L and sodium chloride 3g/L, pH 7.5.Fermentation medium
Ingredient be potato 40.2g/L, beancake powder 15g/L, glucose 2g/L and sodium chloride 5g/L, be adjusted to pH before medium sterilization
7.5.Fermentation condition in fermentor are as follows: 37 DEG C of temperature, pH=7, dissolved oxygen concentration maintains 25% saturation degree, 220rpm.
Comparative example 2
A kind of coptis soil-borne disease control agent provided in this embodiment is water 3-2 parts and 10-25 parts of straw extracting solution, preparation
Method is as follows: preparing straw extracting solution.Autoclave is added after straw is crushed to 2cm, adds 3 times of parts by weight of ethylene glycol,
75 DEG C are heated to, 17min is kept the temperature, collects filtrate, filtrate is distilled, and is stood, is obtained straw extracting solution, then and after water mixing
Obtain the control agent of this comparative example.
In order to verify control agent of the invention to the control efficiency of coptis powdery mildew and southern blight, it is real following field has been carried out
It tests:
The similar coptis seedling of 700 plants of sizes is chosen, seedling leaf is divided into 7 groups, respectively experimental group 1- between 7-10 piece
6 and control group, every group 100 plants, equidistant cultivation is in experimental plot, unified fertilizing management.
Experimental group 1-6 sprays control agent 1000mL prepared by 1-4 of the embodiment of the present invention and comparative example 1-2 respectively, right
According to a group water of sprinkling 1000mL, the control agent of experimental group 1-6 and control group is divided into sprinkling and a pouring root, pouring root three times and is planting
Training carries out after a week, and pouring root dosage is 400mL, and sprinkling control agent carries out after cultivation one month, primary every sprinkling in 15 days, and three
Secondary sprinkling total amount is 600mL, i.e., total dosage of every group control agent is 1000mL, every the growth of three months observation coptis seedling
Situation, and the plant that powdery mildew and southern blight occurs is counted, it is as a result as follows:
Table 1: there are the statistical conditions of the plant number of powdery mildew after cultivating 1 year in coptis seedling
| Group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 1 | Comparative example 2 | Control group |
| After 3 months | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| After 6 months | 0 | 0 | 2 | 1 | 2 | 5 | 7 |
| After 9 months | 1 | 1 | 3 | 3 | 9 | 13 | 12 |
| After 12 months | 3 | 4 | 8 | 5 | 12 | 18 | 19 |
| Disease incidence | 3% | 4% | 8% | 5% | 12% | 18% | 19% |
Table 2: there are the statistical conditions of the plant number of southern blight after cultivating 1 year in coptis seedling
| Group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 1 | Comparative example 2 | Control group |
| After 3 months | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| After 6 months | 0 | 0 | 0 | 0 | 1 | 5 | 3 |
| After 9 months | 0 | 0 | 1 | 2 | 12 | 18 | 15 |
| After 12 months | 1 | 0 | 3 | 5 | 17 | 24 | 23 |
| Disease incidence | 1% | 0% | 3% | 5% | 14% | 24% | 23% |
As can be seen from Table 1, on the whole, the control agent of the embodiment of the present invention 1-4 for the coptis powdery mildew it is anti-
It is relatively obvious to control effect；Wherein the control efficiency of embodiment 1 is best, comparative example 1 have certain control efficiency, but with implementation
Example 1-4 is compared, and control efficiency is relatively poor, and comparative example 2 is compared with control group, and effect is suitable, does not show white to the coptis
Powder disease has apparent control efficiency.
The experimental results showed that individually bacillus subtilis fermentation liquor has certain preventive and therapeutic effect to the powdery mildew of the coptis, it is single
Only straw extracting solution does not show apparent preventive and therapeutic effect to the powdery mildew of the coptis；And bacillus subtilis and straw mention
It is obvious to the control efficiency of coptis powdery mildew after taking liquid weight mixing according to the invention, illustrate straw extracting solution pair
Bacillus subtilis fermentation liquor has synergistic function.
As can be seen from Table 2, on the whole, the control agent of the embodiment of the present invention 1-4 for the coptis southern blight it is anti-
It is relatively obvious to control effect, disease incidence controls within 5%；Wherein the control efficiency of embodiment 2 is best, and comparative example 1 has certain
Control efficiency, but compared with embodiment 1-4, control efficiency is relatively poor, and comparative example 2 is compared with control group, and effect is suitable,
Do not show that there is apparent control efficiency to the white thin,tough silk of the coptis.
The experimental results showed that individually bacillus subtilis fermentation liquor has certain preventive and therapeutic effect to the southern blight of the coptis, it is single
Only straw extracting solution does not show preventive and therapeutic effect to the southern blight of the coptis；And bacillus subtilis and straw extracting solution are pressed
It is obvious to the control efficiency of coptis southern blight after weight mixing of the invention, show straw extracting solution to withered grass bud
Spore bacillus fermentation liquid has synergistic function；
To sum up, the experimental results showed that coptis soil-borne disease control agent of the invention is capable of the powdery mildew of the effectively preventing coptis
And southern blight, wherein the control efficiency of southern blight is more preferably.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of coptis soil-borne disease control agent, which is characterized in that be made of the raw material components of following parts by weight: bacillus subtilis
3-5 parts and 10-25 parts of straw extracting solution of fermented liquid.
2. coptis soil-borne disease control agent as described in claim 1, which is characterized in that by the raw material components system of following parts by weight
At: 3-5 parts and 10-15 parts of straw extracting solution of bacillus subtilis fermentation liquor.
3. coptis soil-borne disease control agent as described in claim 1, which is characterized in that by the raw material components system of following parts by weight
At: 5 parts and 12 parts of straw extracting solution of bacillus subtilis fermentation liquor.
4. the preparation method of the coptis soil-borne disease control agent as described in claim 1-3 any one claim, feature
It is, comprising the following steps:
Step 1: preparing straw extracting solution: autoclave is added after straw is crushed to 2-3cm, adds 2-3 times of its parts by weight
Ethylene glycol is heated to 60-80 DEG C, keeps the temperature 15-30min, collects filtrate, and filtrate is distilled, and stands, obtains straw extracting solution；
Step 2: preparation bacillus subtilis fermentation liquor: NA culture medium is packed into a reservoir, through 115-121 DEG C of high pressure sterilization 20-
40min is cooled to room temperature；The Bacillus subtilis strain that inclined-plane is saved, is seeded on NA culture medium, 37 DEG C, 100-
120rpm shaking table shake culture 16-20h, is made seed liquor；The bacillus subtilis seed liquor of preparation is pressed to the ratio of 0.1-0.2%
Example, which is linked into the fermentor for have been loaded with fermentation medium, cultivates 18-25h；Up to bacillus subtilis bacterium solution；
Step 3: being sufficiently mixed bacillus subtilis fermentation liquor and straw extracting solution according to weight to get institute
The coptis soil-borne disease control agent stated.
5. the preparation method of coptis soil-borne disease control agent as claimed in claim 4, which is characterized in that the NA culture medium
Ingredient is peptone 26g/L, beef extract 5g/L and sodium chloride 3g/L, pH 7.5.
6. coptis soil-borne disease control agent as claimed in claim 4 and preparation method thereof, which is characterized in that the fermented and cultured
The ingredient of base is potato 40.2g/L, beancake powder 15g/L, glucose 2g/L and sodium chloride 5g/L, is adjusted to before medium sterilization
pH 7.5。
7. coptis soil-borne disease control agent as claimed in claim 4 and preparation method thereof, which is characterized in that in the fermentor
Fermentation condition are as follows: 37 DEG C of temperature, pH=7, dissolved oxygen concentration maintains 20-30% saturation degree, 220rpm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114436705A (en) * | 2022-03-18 | 2022-05-06 | 河南省驻科生物科技有限公司 | Liquid fertilizer for increasing yield and preventing and treating plant diseases and insect pests and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101040620A (en) * | 2007-04-24 | 2007-09-26 | 郝锁振 | Method for preparing preparation for preventing and treating cotton verticillium wilt by using crop straws |
| CN103194415A (en) * | 2013-04-24 | 2013-07-10 | 牛赡光 | Bacillus subtilis and application thereof |
| CN103589671A (en) * | 2013-11-12 | 2014-02-19 | 无锡亚克生物科技有限公司 | Bacillus subtilis LNXM37 and application thereof |
-
2018
- 2018-12-25 CN CN201811586318.7A patent/CN109497108A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101040620A (en) * | 2007-04-24 | 2007-09-26 | 郝锁振 | Method for preparing preparation for preventing and treating cotton verticillium wilt by using crop straws |
| CN103194415A (en) * | 2013-04-24 | 2013-07-10 | 牛赡光 | Bacillus subtilis and application thereof |
| CN103589671A (en) * | 2013-11-12 | 2014-02-19 | 无锡亚克生物科技有限公司 | Bacillus subtilis LNXM37 and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114436705A (en) * | 2022-03-18 | 2022-05-06 | 河南省驻科生物科技有限公司 | Liquid fertilizer for increasing yield and preventing and treating plant diseases and insect pests and preparation method thereof |
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