CN109089957A - A kind of cultural method and device improving zebra fish juvenile fish survival rate - Google Patents
A kind of cultural method and device improving zebra fish juvenile fish survival rate Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01K61/00—Culture of aquatic animals
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- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of cultural methods and device for improving zebra fish juvenile fish survival rate.Cultural method includes: to select healthy zebra fish adult fish as parent；Male and female zebra fish is taken to be put into breeding in breeding cylinder；The embryo collected in breeding cylinder lower layer is put into box for breeding, is dispelled embryo with disposable dropper, rejects embryo dead in incubator in time；Embryo is hatched, after juvenile fish all hatching, with yolk water feeding；Feeding yolk water (7-10 days), after use the fairy shrimp feeding juvenile fish of fresh hatching instead.Cultivation apparatus is box for breeding, including cabinet, upper spacer, lower clapboard and horn-like feeding mouth；Upper spacer and lower clapboard are placed in parallel respectively in the protrusion of box for breeding cabinet wall setting, and cabinet is divided into upper, middle, and lower part；Feeding mouth is fixed on upper spacer；Upper spacer and lower clapboard are equipped with sieve pore.The survival rate that can significantly improve zebra fish juvenile fish is cultivated to zebra fish using cultural method and device of the invention, it is at low cost, it is easy to operate.
Description
Technical field
The present invention relates to technical field of aquaculture more particularly to a kind of cultivation sides for improving zebra fish juvenile fish survival rate
Method.
Background technique
Zebra fish is a kind of typical freshwater fish, due to it is smaller with embryo size, transparent, be easy to raise, yield it is high,
The features such as growth cycle is shorter, and production capacity is lasting, and aquaculture cost is low, and experiment consumptive material is few makes zebra fish become fish species poison
Manage the model of research.There is uniform blue stripe on the body of zebra fish, tail fin is extended down to by mouth, shape is fusiform
Back-shaped and transverse compression.Zebra fish length is up to 6.4cm, but seldom growth is greater than 4cm.The service life of zebra fish generally arrives two
In 3 years, it can extend under ideal conditions 5 years.Similar in the structure and function of fish and vertebrate organs, therefore, use
Intoxicating effect that noxious material generates fish predicts the toxic effect to vertebrate.Zebra fish is a kind of common section again
Learn development and gene function that model organism is used to study vertebrate.Growth and development and gene of the researcher using zebra fish
Index etc. is used as toxic endpoints, has played important role in terms of the development toxicity of investigation early life stage toxic compounds.
Fish embryo experiment is considered as a kind of interior animal experiment of smaller pain, therefore is accepted as a kind of substitution other types
Zoopery.
Patent No. CN201210237027.3 has invented a kind of method for breeding of zebra fish, Patent No.
CN201510088196.9 illustrates a kind of propagation method of zebra fish.Two methods mainly establish zebra fish adult fish raising and
Breeding system does not refer to the method for improving juvenile fish survival rate.The propagation method of zebra fish in the prior art, mainly by raising
Vatting bottom place oviposition net cage collect fertilized eggs, the method will lead to zebra fish fertilized egg stick together the death rate height, juvenile fish
The low problem of survival rate.Patent No. CN201711320960.6 has invented a kind of device and method for improving zebra fish rate of fertilization,
But the antioxidant of 40~60 μ g/100g is injected intraperitoneally in every female zebra fish daily during the method needs to cultivate, troublesome in poeration,
And zebra fish spawning rate, embryonic hatching and juvenile fish are different to the sensitivity of drug.The zebra that each research institution uses at present
Fish culture and propagation method difference, every batch of embryo quality is different, and juvenile fish survival rate and quality are different, eventually leads to experimental result
Poor repeatability.Therefore it provides a kind of cultural method easy to operate, reducing be fertilized egg mortality and raising juvenile fish survival rate is especially heavy
It wants.
Summary of the invention
The survival rate that above-mentioned method in the prior art all has raising zebra fish juvenile fish is low, and some can only even survive
40%, for the experiment of application zebra fish, cost is greatly increased, or even influences experiment progress.
In order to solve the above technical problems, providing one the technical problem to be solved by the present invention is to overcome the deficiencies in the prior art
Kind is easy to operate, reduces the device and method of the zebra fish juvenile fish death rate.
In order to solve the above technical problems, the present invention provides a kind of cultural method for improving zebra fish juvenile fish survival rate, packet
Include following steps:
(1) select that the age of a fish is 4~6 months, body is about the healthy zebra fish adult fish of 2.0~3.0cm as parent, in ultraviolet
It is cultivated in the cyclic water tank of disinfection；Condition of culture are as follows: for the water temperature control in water tank at 26 ± 1 DEG C or so, pH is 6.85 ± 1, is protected
Demonstrate,prove 14h illumination and 12h dark separately, and ultraviolet disinfection is sterilized, female milter is separately cultivated；
(2) in breeding the previous day, taking female milter ratio is that the zebra fish of 1:2 is put into breeding cylinder, and is covered with black-out cloth
Cylinder is bred, dark condition raises 12h, then removes light-blocking cloth, keeps indoor quiet, after 2~4h of light stimulation, adult fish is taken
Out, reproductive process is completed；
(3) with suction pipe collect breeding cylinder lower layer in embryo be put into box for breeding, pick out it is transparent, normotrophic by
Smart ovum, quantity are no more than 100 pieces of embryos, are dispelled embryo with disposable dropper, make between embryo not stick to each other, reject in time
Dead embryo in box for breeding；
(4) box for breeding illumination and interlunation are respectively 12h and 12h alternating, and temperature setting is 28 DEG C, zebra fish after 72h
Hatching larvae, after juvenile fish all hatching, juvenile fish is in the opening phase within the 2-3 days, the critical period survived at this time for juvenile fish, with one
Secondary property dropper instills 5ml yolk water on first layer partition, attention dispersion feeding, increases juvenile fish and ingests range；Daily feeding 3
It is secondary, remaining yolk water is put into 4 DEG C of refrigerators and is saved；Yolk is easy to pollution water quality, and timing replacement culture water clears up lower layer
Doping in partition prevents water quality deterioration from influencing juvenile fish existence；Feeding yolk is after 7-10 days with the fairy shrimp feeding children of fresh hatching
Fish.
In the above method, preferably, the recirculated water in the step (1) in cyclic water tank is through sponge, active carbon and coral
The filtering of coral sand, and water body is sufficiently aerated.
Preferably, the temperature of water body in box for breeding is controlled after step (3) the juvenile fish hatching is 25~28 DEG C, and water quality is in weak
Alkalinity, i.e. pH value are controlled 7~8.
Preferably, step (4) the mesolecithal water is rinsed back and forth by wrapping well-done yolk in beaker with bolting silk
It is made.
Preferably, step (4) zebra fish feeding 3 times in the morning, afternoon and evening daily；More there is the time of the feeding of choosing to be respectively as follows: 8:
00,13:00,18:00.
Preferably, the incubation condition of the fairy shrimp is 28 DEG C, continuous aeration culture 24 hours.
Preferably, before fairy shrimp hatching, the canned brine shrimp eggs in refrigerator to be taken out in advance, prepares incubation fluid, good year
Worm incubation fluid is formulated by brine shrimp eggs, sea salt and pure water, and the ratio of the brine shrimp eggs, sea salt and pure water is 3-5 grams:
20-25 grams: 2 liters.It also needs to install heating rod, gas stone, temperature probe before hatching, heating rod power supply is connected when hatching, is opened
Begin to hatch.
Preferably, it feeds fish: the fairy shrimp removed after hatching is poured into wash bottle, be diluted to concentration appropriate and carry out feeding fish.
It is careful not to pour into the chorion of beaker bottom in wash bottle, fish may be dead because of digestive system blocking after eating chorion, greatly
Measuring the chorion not eaten also can polluted-water.
The present invention also provides a kind of cultivation apparatus for improving zebra fish juvenile fish survival rate.
A kind of cultivation apparatus improving zebra fish juvenile fish survival rate, which is characterized in that the cultivation apparatus is box for breeding, packet
It includes cabinet (1), upper spacer (2), lower clapboard (3) and horn-like feeding mouth (4)；The upper spacer (2) and lower clapboard (3) are put down respectively
Row is placed on the upper limit raised (5) and lower limit raised (6) of cultivation box body (1) inner wall setting, cabinet is divided into,
In, lower three parts；The horn-like feeding mouth (4) is fixed on upper spacer (2)；The upper spacer (2) and lower clapboard (3) are all provided with
Have sieve pore (7), the diameter of the sieve pore (7) is less than the outer diameter of zebrafish embryo；Cabinet top is used for feeding yolk water, in cabinet
Portion is for cultivating zebra fish juvenile fish；Lower box is used to store the swill and excreta of sedimentation.
Preferably, the diameter of the sieve pore is 0.1cm.
The device upper spacer for preventing the biggish yolk water of partial size from feeding in box for breeding, to prevent influence juvenile fish it is edible and
Polluted-water.Horn-like feeding mouth can facilitate the feeding of fairy shrimp.It is detachably connected between partition and cultivation cabinet, it is easy to clean
With replacement, aquaculture cost is reduced.Swill and excreta can be deposited to box for breeding lower box by box for breeding lower clapboard, be separated
It is lethal into cotton-shaped pollutant to guarantee that juvenile fish will not twine for juvenile fish and pollutant.
The present invention has a characteristic that
(1) cultural method of the invention can guarantee the health being frequently grown the day of zebra fish, meet the growth rule of zebra fish
Rule, can guarantee the survival and growth of zebra fish high quality.
(2) cultivation apparatus structure of the invention is simple, and cost is relatively low, convenient maintenance.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of apparatus of the present invention.
In figure: 1 cabinet, 2 upper spacers, 3 lower clapboards, 4 feeding mouths, 5 upper limits protrusion, 6 lower limits protrusion, 7 sieve pores.
Specific embodiment
Below by specific embodiment, and in conjunction with attached drawing, the technical solutions of the present invention will be further described.
In the present invention, if not refering in particular to, all devices and raw material is commercially available or the industry is common are following
Method in embodiment is unless otherwise instructed conventional method in that art.
One, it selects adult fish parent: selecting the healthy zebra fish adult fish that the age of a fish is about 2.0~3.0cm for 4~6 months, body and make
For parent, cultivated in the cyclic water tank of ultraviolet disinfection；Condition of culture are as follows: the water temperature control in cyclic water tank is at 26 ± 1 DEG C, pH
It is 6.85 ± 1, guarantees 14h illumination and 12h dark separately, and ultraviolet disinfection is sterilized, female milter is separately cultivated；In cyclic water tank
Recirculated water filtered through sponge, active carbon and coral sand, and water body is sufficiently aerated.
Two, obtain zebrafish embryo: in breeding the previous day, taking female milter ratio is that the zebra fish of 1:2 is put into breeding cylinder,
And breeding cylinder is covered with black-out cloth, and dark condition raises 12h, then removes light-blocking cloth, keep indoor quiet, light stimulation 2~
After 4h, adult fish is taken out；
Three, incubation condition: illumination and interlunation are respectively 12h and 12h alternating, and temperature setting is 28 DEG C.
Four, cultivation apparatus:
As shown in Figure 1, a kind of cultivation apparatus for improving zebra fish juvenile fish survival rate, the cultivation apparatus is box for breeding, packet
It includes cabinet (1), upper spacer (2), lower clapboard (3) and horn-like feeding mouth (4)；The upper spacer (2) and lower clapboard (3) are put down respectively
Row is placed on the upper limit raised (5) and lower limit raised (6) of cultivation box body (1) inner wall setting, cabinet is divided into,
In, lower three parts；The horn-like feeding mouth (4) is fixed on upper spacer (2)；The upper spacer (2) and lower clapboard (3) are all provided with
Have sieve pore (7), the diameter of the sieve pore (7) is less than the outer diameter of zebrafish embryo；Cabinet top is used for feeding yolk water, in cabinet
Portion is for cultivating zebra fish juvenile fish；Lower box is used to store the swill and excreta of sedimentation.The diameter of the sieve pore is
0.1cm。
Embodiment 1
(1) by 50 pieces of zebrafish embryo cultivation in the middle part of the cabinet of the device of above-mentioned raising juvenile fish survival rate, with disposable
Dropper dispels embryo, makes between embryo not stick to each other, rejects embryo dead in incubator in time；
(2) zebrafish embryo is hatched, zebrafish larvae is about all hatched after 72h；
(3) temperature of water body is 25 DEG C in control device after hatching, and water quality alkalescent pH value is controlled 7；Juvenile fish was from the 1st day
Feeding yolk water, with the fairy shrimp feeding juvenile fish of fresh hatching after 7 days；
(4) it tests the culture-cycle one month, counts the survival rate of juvenile fish.
Embodiment 2
(1) by 70 pieces of zebrafish embryo cultivation in the middle part of the cabinet of the device of above-mentioned raising juvenile fish survival rate, with disposable
Dropper dispels embryo, makes between embryo not stick to each other, rejects embryo dead in incubator in time；
(2) zebrafish embryo is hatched, zebrafish larvae is about all hatched after 72h；
(3) temperature of water body is 26 DEG C in control device after hatching, and water quality alkalescent pH value is controlled 7.5；Juvenile fish is from the 2nd
Its feeding yolk water, with the fairy shrimp feeding juvenile fish of fresh hatching after 8 days；
(4) it tests the culture-cycle one month, counts the survival rate of juvenile fish.
Embodiment 3
(1) by 80 pieces of zebrafish embryo cultivation in the middle part of the cabinet of the device of above-mentioned raising juvenile fish survival rate, with disposable
Dropper dispels embryo, makes between embryo not stick to each other, rejects embryo dead in incubator in time；
(2) zebrafish embryo is hatched, zebrafish larvae is about all hatched after 72h；
(3) temperature of water body is 27 DEG C in control device after hatching, and water quality alkalescent pH value is controlled 7.5；Juvenile fish is from the 3rd
Its feeding yolk water, with the fairy shrimp feeding juvenile fish of fresh hatching after 10 days；
(4) it tests the culture-cycle one month, counts the survival rate of juvenile fish.
Comparative example
(1) by 80 pieces of zebrafish embryo cultivation in the common culturing jar of no partition and feeding hole, with disposable dropper
Embryo is dispelled, makes between embryo not stick to each other, rejects embryo dead in incubator in time；
(2) zebrafish embryo is hatched, zebrafish larvae is about all hatched after 72h；
(3) temperature of water body is 27 DEG C in control device after hatching, and water quality alkalescent pH value is controlled 7.5；Juvenile fish is from the 3rd
Its feeding yolk water, with the fairy shrimp feeding juvenile fish of fresh hatching after 10 days；
(4) it tests the culture-cycle one month, counts the survival rate of juvenile fish.
The survival rate of the zebra fish of embodiment 1-3 and comparative example 1 is counted, the results are shown in Table 1:
1. zebra fish survival rate result of table
As can be seen from Table 1, the survival rate of zebra fish juvenile fish is apparently higher than zebra fish children in comparative example 1 in embodiment 1-3
The survival rate of fish.Therefore cultivation apparatus of the invention is conducive to improve the survival rate of zebra fish juvenile fish.
The method of the present invention that the above embodiments are only used to help understand and its core concept.It should be pointed out that for
For those skilled in the art, without departing from the principle of the present invention, if can also be carried out to the present invention
Dry improvement and modification, these improvement and modification are also fallen into the claims in the present invention protection scope.
Claims (10)
1. a kind of cultural method for improving zebra fish juvenile fish survival rate, which comprises the following steps:
(1) select that the age of a fish is 4~6 months, body is about the healthy zebra fish adult fish of 2.0~3.0cm as parent, in ultraviolet disinfection
Cyclic water tank in cultivate；Condition of culture are as follows: for the water temperature control in cyclic water tank at 26 ± 1 DEG C, pH is 6.85 ± 1, guarantees 14h
Separately, and ultraviolet disinfection is sterilized for illumination and 12h dark, and female milter separately cultivates；
(2) in breeding the previous day, taking female milter ratio is that the zebra fish of 1:2 is put into breeding cylinder, and covers breeding with black-out cloth
Cylinder, dark condition raise 12h, then remove light-blocking cloth, keep indoor quiet, after 2~4h of light stimulation, fish is taken out, is completed
Reproductive process；
(3) embryo in breeding cylinder lower layer is collected with suction pipe to be put into box for breeding, pick out transparent, normotrophic fertilization
Ovum, quantity are no more than 100 pieces of embryos, are dispelled embryo with disposable dropper, make between embryo not stick to each other, reject training in time
Support embryo dead in case；
(4) box for breeding illumination and interlunation are respectively 12h and 12h alternating, and temperature setting is 28 DEG C, zebrafish larvae after 72h
Hatching instills 5ml yolk water with disposable dropper, attention dispersion is thrown after juvenile fish all hatching on box for breeding upper spacer
Food increases juvenile fish and ingests range；Daily feeding 3 times, timing replacement culture water clears up the residue in lower clapboard；Feeding yolk water
Use the fairy shrimp feeding juvenile fish of fresh hatching after 7-10 days instead.
2. a kind of cultural method for improving zebra fish juvenile fish survival rate according to claim 1, which is characterized in that the step
Suddenly the recirculated water in (1) cyclic water tank is filtered through sponge, active carbon and coral sand, and water body is sufficiently aerated.
3. a kind of cultural method for improving zebra fish juvenile fish survival rate according to claim 1, which is characterized in that the step
Suddenly it is 25~28 DEG C that the temperature of water body in box for breeding is controlled after the hatching of (3) juvenile fish, and pH value is 7~8.
4. a kind of cultural method for improving zebra fish juvenile fish survival rate according to claim 1, which is characterized in that the step
Suddenly (4) mesolecithal water by with bolting silk wrap well-done yolk in beaker back and forth rinsing be made.
5. a kind of cultural method for improving zebra fish juvenile fish survival rate according to claim 1, which is characterized in that the step
Suddenly (4) zebra fish is pressed feeding in the morning, afternoon and evening 3 times daily.
6. a kind of cultural method for improving zebra fish juvenile fish survival rate according to claim 1, which is characterized in that described rich
The incubation condition of year worm is 28 DEG C, continuous aeration culture 24 hours.
7. a kind of cultural method for improving zebra fish juvenile fish survival rate according to claim 6, which is characterized in that fairy shrimp
Incubation fluid is formulated by brine shrimp eggs, sea salt and pure water, and the ratio of the brine shrimp eggs, sea salt and pure water is 3-5 grams: 20-
25 grams: 2 liters.
8. a kind of cultural method for improving zebra fish juvenile fish survival rate according to claim 6, which is characterized in that feed fish:
Fairy shrimp after hatching is poured into wash bottle, concentration appropriate is diluted to and carries out feeding fish.
9. a kind of cultivation apparatus for improving zebra fish juvenile fish survival rate, which is characterized in that the cultivation apparatus is box for breeding, including
Cabinet (1), upper spacer (2), lower clapboard (3) and horn-like feeding mouth (4)；The upper spacer (2) is parallel respectively with lower clapboard (3)
Be placed on the upper limit raised (5) and lower limit protrusion (6) of cultivation box body (1) inner wall setting, cabinet is divided into, in,
Lower three parts；The horn-like feeding mouth (4) is fixed on upper spacer (2)；The upper spacer (2) and lower clapboard (3) are equipped with
Sieve pore (7), the diameter of the sieve pore (7) are less than the outer diameter of zebrafish embryo；Cabinet top is used for feeding yolk water, cabinet middle part
For cultivating zebra fish juvenile fish；Lower box is used to store the swill and excreta of sedimentation.
10. a kind of cultivation apparatus for improving zebra fish juvenile fish survival rate according to claim 9, which is characterized in that described
The diameter of sieve pore is 0.1cm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110063275A (en) * | 2019-03-04 | 2019-07-30 | 中国环境科学研究院 | A kind of zebra fish cultural method stage by stage |
| CN111631176A (en) * | 2020-07-22 | 2020-09-08 | 郑昊 | Fry hatching pond device is used to agricultural |
| CN111670846A (en) * | 2020-04-20 | 2020-09-18 | 厦门大学 | In-situ cultivation method for sponge larvae |
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| CN104719196A (en) * | 2015-02-26 | 2015-06-24 | 湖南省植物保护研究所 | Breeding method of zebra fish |
| CN108056058A (en) * | 2017-12-12 | 2018-05-22 | 浙江海洋大学 | A kind of device and method for improving zebra fish rate of fertilization |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110063275A (en) * | 2019-03-04 | 2019-07-30 | 中国环境科学研究院 | A kind of zebra fish cultural method stage by stage |
| CN111670846A (en) * | 2020-04-20 | 2020-09-18 | 厦门大学 | In-situ cultivation method for sponge larvae |
| CN111670846B (en) * | 2020-04-20 | 2021-10-15 | 厦门大学 | In-situ cultivation method for sponge larvae |
| CN111631176A (en) * | 2020-07-22 | 2020-09-08 | 郑昊 | Fry hatching pond device is used to agricultural |
| CN111631176B (en) * | 2020-07-22 | 2022-02-15 | 雷州海茂水产种业科技有限公司 | Fry hatching pond device is used to agricultural |
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Application publication date: 20181228 |