CN102578006B - Annual culturing method for marine medaka in lab - Google Patents
Annual culturing method for marine medaka in lab Download PDFInfo
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- CN102578006B CN102578006B CN201110434197.6A CN201110434197A CN102578006B CN 102578006 B CN102578006 B CN 102578006B CN 201110434197 A CN201110434197 A CN 201110434197A CN 102578006 B CN102578006 B CN 102578006B
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 241001417127 Oryzias melastigma Species 0.000 title abstract description 7
- 238000012258 culturing Methods 0.000 title abstract 4
- 239000013535 sea water Substances 0.000 claims abstract description 18
- 238000009395 breeding Methods 0.000 claims abstract description 16
- 230000001488 breeding effect Effects 0.000 claims abstract description 16
- 241000251468 Actinopterygii Species 0.000 claims abstract description 11
- 238000005286 illumination Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 4
- 239000001301 oxygen Substances 0.000 claims abstract description 4
- 241000276569 Oryzias latipes Species 0.000 claims description 29
- 230000003203 everyday effect Effects 0.000 claims description 10
- 241001247197 Cephalocarida Species 0.000 claims description 4
- 239000004677 Nylon Substances 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 230000002070 germicidal effect Effects 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 230000017448 oviposition Effects 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 2
- 241000238582 Artemia Species 0.000 abstract 1
- 239000003463 adsorbent Substances 0.000 abstract 1
- 229920006284 nylon film Polymers 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 235000013601 eggs Nutrition 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000798443 Hornodermoporus martius Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 238000009318 large scale farming Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an annual culturing method for marine medaka in a lab. Culturing conditions include: culture seawater is 28-30 in salinity and 4-8mg/L in dissolved oxygen level after subjected to five-stage filtering via a nylon film, biological adsorbent, activated carbon, biochemical filter and ultraviolet sterilization system; the culture water is at 26-30 DEG C; the culture density reaches 0.2-0.5L per fish in the growth period and reaches 0.5-1L per fish in the breeding period; a light cycle includes 14 hours of illumination and 10 hours of no illumination, and illumination is performed from 5:00 to 19:00; the culture male-to-female ratio is 2:3; in terms of feeding frequency and feeding time, the fishes are fed with marine tropical fish feed three times a day, from 7:30 to 8:30, from 11:30 to 12:30 and from 15:30 to 16:30, and are fed with Artemia larvae twice a day, from 9:45 to 10:15 and from 13:45 to 14:15. By the annual culturing method, the defects that breeding conditions are difficult to control, cost is high, continuous egg laying fails, egg laying amount is low, breeding conditions are limited, and the like are overcome.
Description
Technical field
The present invention relates to the cultural method of a kind of fish, particularly the laboratory anniversary cultural method of the blue or green Medaka of a kind of seawater (having another name called the blue or green Medaka of stain).
Background technology
Lack generally acknowledged ocean model fingerling in the world in order to carry out marine ecology toxicologic study at present.The blue or green Medaka of stain (
oryzias melastigma) at marine site ubiquities such as China, Korea, Japan and India, its generation cycle is short, genome is less, spawning rate is high, be easy to observation, salinity adaptation scope is wide, has the potentiality becoming ocean model biology.The relevant scholar on the ground such as current China, Japan, Taiwan and Korea S and the mechanism multinomial ecological toxicology Study and appliance that it can be used as new test fingerling to carry out.
The blue or green Medaka body of stain is white, and the flat-sided back of the body is short, and there is a pair larger anal fin at health rear portion.Eye is large in blue.Long 3.5 ~ the 4cm of the individual body of sexual maturity.Male and femalely very easily to distinguish from form.The blue or green Medaka of stain is oviparity, and embryo is hatched after 14 days about fertilization greatly at 28 DEG C.The juvenile fish of new hatching is about ripe through 3 months and lay eggs.The blue or green Medaka of stain is distributed in the top layer in Perenniporia martius marine site, likes a large amount of illumination, and illumination is more strong better, but the illumination continued can upset the rhythm and pace of moving things of the blue or green Medaka breeding of stain.The blue or green Medaka of stain can bear the salinity range from 0 ~ 60ppt, and can survive in the scope of 4 ~ 38 DEG C.26 ~ 30 DEG C is the suitableeest the bread worm, and when temperature gradients, the blue or green Medaka of stain can bear a wide range of temperature fluctuation.
There is following problem in the blue or green Medaka of current Laboratory culture stain: 1. cultivating condition difficulty controls and differs; 2. egg laying amount is few, and incubation rate is low; 3. can not continue to lay eggs; 4. easily there is disease; 5. artificial seawater cost is high.
Therefore the cultural method of the blue or green Medaka of a kind of new stain that can solve the problem is needed now.
Summary of the invention
The present invention is the above-mentioned deficiency in order to solve existing for prior art, a kind of method that can realize stain blue or green Medaka adult fish laboratory large-scale farming is proposed, in order to solve, cultivating condition difficulty controls, cost is high, can not continue to lay eggs, egg laying amount is few and Reproduction Conditions exists the blue or green Medaka of seawater (the stain green grass or young crops Medaka of the aspect defects such as restriction
oryzias melastigma) laboratory anniversary cultural method.
Technical solution of the present invention is: the blue or green Medaka of a kind of seawater (the blue or green Medaka of stain,
oryzias melastigma) laboratory anniversary cultural method, it is characterized in that: the cultivating condition of described cultural method is as follows:
Breeding water body: after nylon membrane, biological adsorption agent, active carbon, biochemical filter and UV germicidal system Pyatyi filtration treatment, salinity is 28-30, and dissolved oxygen levels is the seawater of 4-8mg/L
Temperature of cultivation: 26-30 degree Celsius
Cultivation density: vegetative period is 0.2-0.5L/ tail, breeding period is 0.5-1L/ tail
Photoperiod: illumination 14 hours, dark 10 hours, and lighting hours is 5:00-19:00
Cultivation sex ratio: male: female=2:3
Bait throwing in number of times and bait throwing in time: 7:30-8:30,11:30-12:30,15:30-16:30 of every day divide seawater feed for tropical fish of throwing something and feeding for three times, 9:45-10:15,13:45-14:15 of every day throw something and feed artemia larvae at twice.
Compared with the existing technology, tool has the following advantages in the present invention:
The laboratory anniversary cultural method of the blue or green Medaka of seawater proposed by the invention, has relatively strict cultivating condition, and lower cost simulation can be utilized to go out the self-sow environment of the blue or green Medaka of stain, is more conducive to growth and the breeding of the blue or green Medaka of stain; And profit cultivates the blue or green Medaka of stain in this way, can realize the integration of the processes such as raising, breeding and hatching, save time, simplify breeding process, its quantity of laying eggs and quality all increase, for scientific research provides stable guarantee; And this method is simple, be particularly suitable for ecological toxicology research in laboratory model organism cultivation in promote and application, its market prospects and significance of scientific research are all very good.
Embodiment
The specific embodiment of the present invention will be described in detail below.
The blue or green Medaka of a kind of seawater (the blue or green Medaka of stain,
oryzias melastigma) laboratory anniversary cultural method, it is characterized in that: the cultivating condition of described cultural method is as follows:
Breeding water body: the seawater after nylon membrane, biological adsorption agent, active carbon, biochemical filter and UV germicidal system Pyatyi filtration treatment, controlling its salinity is 28-30, and dissolved oxygen levels is 4-8mg/L,
Temperature of cultivation: 26-30 degree Celsius,
Cultivation density: vegetative period is 0.2-0.5L/ tail, the blue or green Medaka of stain growing more than three months enters breeding period, and the cultivation density between breeding period is 0.5-1L/ tail,
Photoperiod: illumination 14 hours, dark 10 hours, and lighting hours is 5:00-19:00
Cultivation sex ratio: male: female=2:3
Bait throwing in number of times and bait throwing in time: 7:30-8:30,11:30-12:30,15:30-16:30 of every day divide seawater feed for tropical fish of throwing something and feeding for three times, 9:45-10:15,13:45-14:15 of every day throw something and feed artemia larvae at twice.
Every day carries out five bait throwing in the blue or green Medaka of stain, 7:30-8:30 is first time bait throwing in, 9:45-10:15 is second time bait throwing in, 11:30-12:30 is third time bait throwing in, 13:45-14:15 is the 4th bait throwing in, 15:30-16:30 is the 5th bait throwing in, and wherein the first, the 3rd and the 5th bait throwing in is thrown something and fed seawater feed for tropical fish, and first time bait throwing in and third time bait throwing in, time interval between third time bait throwing in and the 5th bait throwing in are four hours; The second, the 4th bait throwing in is thrown something and fed artemia larvae, and the time interval between second time bait throwing in and the 4th bait throwing in is similarly four hours; Each bait throwing in adopts a small amount of principle repeatedly, to the blue or green Medaka of stain is no longer ingested;
In order to assist to cultivate illumination, ensure that illumination is sufficient and even, opened by the illuminating lamp that cultivation is indoor at the 8:00 of every day, 17:00 closes illuminating lamp, and the illuminator only by breeding box provides light;
This Laboratory culture method, because water temperature is higher, evaporation of seawater amount is larger, every day, the salinity of breeding water body can slightly improve, therefore for ensureing the stability of salinity, the salinity of water body must be measured every day, when salinity is more than 30, under the condition that filtered water pump is opened, in water body, slowly add appropriate distilled water stablize to maintain salinity.
Under this method can realize the laboratory environment of the blue or green Medaka of seawater (the blue or green Medaka of stain, Oryzias melastigma), large-scale anniversary cultivation.
Claims (1)
1. a laboratory anniversary cultural method for the blue or green Medaka of seawater, is characterized in that: the cultivating condition of described cultural method is as follows:
Breeding water body: after nylon membrane, biological adsorption agent, active carbon, biochemical filter and UV germicidal system Pyatyi filtration treatment, salinity is 28-30, and dissolved oxygen levels is the seawater of 4-8mg/L
Temperature of cultivation: 26-30 degree Celsius
Cultivation density: vegetative period is 0.2-0.5L/ tail, breeding period is 0.5-1L/ tail
Photoperiod: illumination 14 hours, dark 10 hours, and lighting hours is 5:00-19:00
Cultivation sex ratio: male: female=2:3
Bait throwing in number of times and bait throwing in time: 7:30-8:30,11:30-12:30,15:30-16:30 of every day divide seawater feed for tropical fish of throwing something and feeding for three times, 9:45-10:15,13:45-14:15 of every day throw something and feed artemia larvae at twice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110434197.6A CN102578006B (en) | 2011-12-22 | 2011-12-22 | Annual culturing method for marine medaka in lab |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110434197.6A CN102578006B (en) | 2011-12-22 | 2011-12-22 | Annual culturing method for marine medaka in lab |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102578006A CN102578006A (en) | 2012-07-18 |
| CN102578006B true CN102578006B (en) | 2015-02-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110434197.6A Active CN102578006B (en) | 2011-12-22 | 2011-12-22 | Annual culturing method for marine medaka in lab |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111134056B (en) * | 2020-01-10 | 2021-08-31 | 山东省海洋科学研究院 | Method for hatching fertilized eggs of medaka in seawater |
| CN112293306B (en) * | 2020-11-09 | 2022-03-08 | 闽江学院 | Method for preparing aseptic sea water green larval fish |
| CN114287366A (en) * | 2021-12-02 | 2022-04-08 | 重庆绵凯生物技术研究院有限公司 | Production method and application of sterile seawater medaka adult fish |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828545A (en) * | 2009-03-09 | 2010-09-15 | 中国水产科学研究院东海水产研究所 | Anhydrous transportation method for medaka zygotes |
| CN101831400A (en) * | 2009-03-09 | 2010-09-15 | 中国水产科学研究院东海水产研究所 | Method for collecting and separating medaka zygotes |
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- 2011-12-22 CN CN201110434197.6A patent/CN102578006B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828545A (en) * | 2009-03-09 | 2010-09-15 | 中国水产科学研究院东海水产研究所 | Anhydrous transportation method for medaka zygotes |
| CN101831400A (en) * | 2009-03-09 | 2010-09-15 | 中国水产科学研究院东海水产研究所 | Method for collecting and separating medaka zygotes |
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| CN102578006A (en) | 2012-07-18 |
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