CN108935842A - A kind of preparation and application of balsam pear blue or green money willow lipid-lowering health-care tea - Google Patents
A kind of preparation and application of balsam pear blue or green money willow lipid-lowering health-care tea Download PDFInfo
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- CN108935842A CN108935842A CN201810695020.3A CN201810695020A CN108935842A CN 108935842 A CN108935842 A CN 108935842A CN 201810695020 A CN201810695020 A CN 201810695020A CN 108935842 A CN108935842 A CN 108935842A
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- 230000004060 metabolic process Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229960000292 pectin Drugs 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- GPPXJZIENCGNKB-UHFFFAOYSA-N vanadium Chemical compound [V]#[V] GPPXJZIENCGNKB-UHFFFAOYSA-N 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the preparations and application of a kind of balsam pear blue or green money willow lipid-lowering health-care tea, its main component includes the auxiliary materials such as Momordica charantial extract, blue or green money willow polyoses extract and xylitol, citric acid, stevioside, and particle electuary, oral solution, drink, capsule or tablet can be made in when production；The present invention also provides study the health protection tea in lipid-loweringing, pre- preventing obesity, anti-oxidant, in terms of anti-aging application using Caenorhabditis elegans model.The advantages that health composition of the invention has and has no toxic side effect based on natural extract active object, and synergistic effect is obvious, easy to use, suitable population is used for a long time.
Description
Technical field
The present invention relates to technical field of health care food, and in particular to a kind of preparation of balsam pear blue or green money willow lipid-lowering health-care tea and answers
With.
Background technique
Balsam pear is a kind of Curcurbitaceae balsam pear platymiscium, and in China, cultivated area is wide, wide in variety, and resource is extremely abundant, balsam pear
It is a kind of plant of integration of drinking and medicinal herbs, there is long history in terms of pharmacology.It is rich in Momordica charantial, polypeptide, flavone compound
Etc. a variety of active ingredients, have relieve summer heat quench one's thirst, fall fire is reduced phlegm and internal heat, hypoglycemic, reducing blood lipid, it is anti-oxidant, enhancing immunity of organisms, promote
The extensive pharmacological action such as metabolic and pre- preventing obesity and health value.Active constituent in balsam pear, which has, promotes fat oxidation
With the effect of glucose utilization, have effects that the rouge that disappears of losing weight well, therefore is known as " fatty killer ", but due to balsam pear
It is in sweetness and bitterness taste containing the ingredients such as Momordia grosvenori aglycone and Charantin, so that the development and utilization of balsam pear are very limited, it is domestic at present
Outside to balsam pear hypoglycemic in terms of have more comprehensive research, but the research in terms of lipid-loweringing is less.
Blue or green money willow is cyclocarya plant, is distributed widely in the Yangtze river basin, is the distinctive natural resources in China.It is civil common
Its tender leaf is made tea, sweet tea sweet in flavor, also known as Sweet tea, is a kind of natural resources of Chinese medicinal materials of preciousness, blue or green money willow saponin(e rich in, flavones,
The Organic nutrients such as polysaccharide, the inorganic nutrients ingredient such as a large amount of iron, zinc, selenium, chromium, germanium, magnesium, calcium, also containing manganese, iron, copper,
The microelements such as chromium, zinc, selenium, vanadium, germanium.Research shows that the extract of blue or green money willow has hypoglycemic, reducing blood lipid, blood pressure lowering, antioxygen
The remarkable activities such as change, anti-aging, and without acute, chronic toxic action, wherein existing research shows that blue or green money willow polysaccharide passes through adjusting
With lipid metabolism related gene expression, inhibits liver lipids oxidation and pancreatic lipase activity, play and significantly facilitate blood lipid metabolism
Effect, therefore it is furtherd investigate with exploitation have important theoretical value and application prospect.
1997 the World Health Organization (WHO) obesity is defined as a kind of disease, as rhythm of life is accelerated, diet structure
Change, high in fat and high protein foods intake day is cumulative, and obesity is in the trend being gradually increasing, and obesity is due to extraneous ring
A kind of common disease caused by border factor or human body intrinsic factor, fatty over-deposit can cause metabolic syndrome, cardiovascular and cerebrovascular
The chronic diseases such as disease and three high diseases (hypertension, hyperlipidemia, hyperglycemia), the main population's first is that fat of three high diseases morbidity
Person, and that there is awarenesses is low, serve organization is low, control rate is low, and illness rate is high, disability rate is high, the death rate is high, to society and family
Bring serious burden in front yard, it has also become threaten " the first killer " of health of people.
As the market of weight-reducing and lipid-lowering product is gradually expanded, occur many antihyperlipidemic products such as Statins, fibrates etc. at present
Drug, although having certain lipid-lowering effect, toxic side effect is also larger, serious or even will affect the phases such as liver, kidney, the heart, eye
The function problem for closing device, influences the control of quality of life.With getting worse for problem of obesity, urgent need is quickly effective, treats
Obvious, few side effects weight-reducing modes are imitated, natural health-care products are highly-safe, have apparent advantage and future in contrast
The direction of development, therefore the functional food with effect for reducing fat is researched and developed to prevention and treatment obesity with highly important
Meaning.
Summary of the invention
The purpose of the present invention is to provide the preparations and application of a kind of balsam pear blue or green money willow lipid-lowering health-care tea, to solve above-mentioned back
The problem of being proposed in scape technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of balsam pear blue or green money willow lipid-lowering health-care tea, the raw material including following parts by weight:
Momordica charantial extract 0.1~10%, blue or green money willow polyoses extract 5~35%, xylitol 1~10%, citric acid
0.1~2%, stevioside 0.01~1%.
Be as further scheme of the invention: the balsam pear blue or green money willow lipid-lowering health-care tea includes the original of following parts by weight
Material:
Momordica charantial extract 0.1%, blue or green money willow polyoses extract 10%, xylitol 2.25%, citric acid 0.12%, sweet tea
Synanthrin 0.02%.
It is as further scheme of the invention: is also added in the balsam pear blue or green money willow lipid-lowering health-care tea acceptable auxiliary
Material.
Be as further scheme of the invention: the acceptable auxiliary material is fructose syrup, potassium sorbate, white sand
Sugar, citric acid, malic acid, sodium citrate, calgon, xylitol, stevioside, sucrose, pregelatinized starch, Aspartame,
Beta-cyclodextrin, agar, pectin, vitamin C, vitamin B, vitamin D, xylitol, hydroxypropyl PASELLI EASYGEL, oligomeric different wheat
One of bud sugar or a variety of compositions.
Be as further scheme of the invention: the health protection tea be particle electuary, oral solution, drink, capsule or
Tablet.
Be as further scheme of the invention: the preparation method of the balsam pear blue or green money willow lipid-lowering health-care tea includes following
Step:
Step 1: the extraction of Momordica charantial
Pretreatment of raw material: select appearance it is normal, without rot, the balsam pear without insect pest, half deseeding is cut after cleaning, is sliced, goes forward side by side
Row color protection and de- suffering are managed, and 12h is dried at 55 DEG C, and moisture content is 4~5%, are pulverized, are crossed 60 meshes, be stored in drier
In it is stand-by；
It extracts: balsam pear powder is added to 70% ethyl alcohol for being preheating to certain temperature by 1: 20 solid-liquid ratio, be 60 in extraction temperature
DEG C, extracting pressure 0.5MPa, resolution temperature is 60 DEG C, and extraction time carries out low temperature continuous phase transistion extraction under the conditions of being 60min,
It is centrifuged, filtered, centrifugal speed 4000r/min, centrifugation time collect filter residue and filtrate respectively after being 5min, filter residue carries out same
The second extraction of batten part, the filtrate extracted twice is merged and is concentrated into the 1/5 of original volume, and drying washing 3 times obtains after dry
Momordica charantial extract, recovery rate 12.58%, saponin(e purity are 64.5%, and 4 DEG C save backup；
Step 2: the extraction of blue or green money willow polysaccharide
Pretreatment of raw material: selecting that appearance is normal, disease-free blue or green money willow, removes limb, shreds after being cleaned, at 55 DEG C
12h is dried, moisture content is 3~4%, and stand-by after 40 meshes of pulverizing；
It extracts: blue or green money willow powder ultrasonic wave added hot water being extracted into polysaccharide, solid-liquid ratio 1: 10, temperature is 85 DEG C, is mentioned
It takes 3 times, each 2h, filtrate is concentrated by evaporation to the 1/4 of original volume after suction filtration, be placed in 4 DEG C of alcohol precipitations and stay overnight, be in centrifugal speed
It abandons supernatant after 3500r/min, centrifugation 10min to carry out being freeze-dried to obtain crude extract, and by crude extract with 80% ethanol washing three times
After be freeze-dried up to blue or green money willow polyoses extract, recovery rate 10.25%, purity 78.2%, molecular weight 900kDa
~1160kDa, 4 DEG C save backup；
Step 3: balsam pear-Qingqian willow health tea development process
The extract and xylitol, citric acid, stevioside auxiliary material of step 1 and step 2 are dissolved in water allotment in proportion
Uniformly, it uses boiling water bath to sterilize 15min after homogeneous and degassing, carries out hot filling at once, cover, then use sub-sectional cooling, directly
To close to room temperature, finally examining various microbiological indicators and physicochemical property and being stored, oral liquid formulation health protection tea is made；
Step 4: particle electuary is made using the method for spray drying in oral liquid formulation health protection tea made from step 3 and is protected
Health tea is made in strong tea, or the syrup of 4 times of volumes of addition；
Step 5: particle electuary health protection tea made from step 4 is subjected to pulverization process through pulverizer, pulverizer revolving speed is
8000r/min, then syrup is directly sprayed onto tea powder through atomizing pelletizer, the fine particle formed is packed into capsule shell shape
Tabletted under 5t pressure at capsule or repressed molding machine, cooling down and wrapping sweet tablet forms tablet.
Be as further scheme of the invention: processing condition is in high pressure homogenizer 20MPa item in the step 3
Homogeneous 2 times under part, sub-sectional cooling condition are respectively with 75 DEG C, 50 DEG C, tap water at normal temperature sub-sectional cooling.
Be as further scheme of the invention: routine can be used in the Momordica charantial and blue or green money willow polysaccharide active components
Extracting method extract.
The present invention also provides a kind of balsam pear blue or green money willow lipid-lowering health-care teas in lipid-loweringing, pre- preventing obesity, anti-oxidant, anti-aging
In application.
Be as further scheme of the invention: its described lipid-loweringing, pre- preventing obesity, anti-oxidant and what is delayed senescence comments
Valence system includes Caenorhabditis elegans model.
Compared with prior art, the present invention have it is following the utility model has the advantages that
(1) effect for reducing fat of the present invention using Caenorhabditis elegans model research balsam pear Qingqian willow health tea, caenorhabditis elegan
There is outstanding advantages of culture is simple, life cycle is short, whole genome sequence is clear, highly conserved in evolution, and in lactation
It is also conservative existing in nematode that the factor and metabolic pathway in service life and fat metabolism are adjusted in animal, therefore nematode is fat
With the good animal model of life search, the effect for reducing fat of balsam pear Qingqian willow health tea is studied using caenorhabditis elegan model, for hardship
The lipid-loweringing research of melon saponin(e and blue or green money willow polysaccharide provides theoretical basis, has to prevention or the Related product for treating fat disease
Important directive function.
(2) domestic and foreign scholars have made great progress the research of the active constituent, healthcare function of balsam pear and blue or green money willow,
But effect for reducing fat research combined for the two is very few, at present on the market also without the combined auxiliary drop of the two
The product of blood lipid, the present inventor extract Momordica charantial and blueness from balsam pear and blue or green money willow by furtheing investigate and practicing for a long time
Two kinds of ingredients are combined together by creative labor, develop a Antilipemic health composition by money willow polysaccharide natural component
And its preparation, both be effectively utilized reducing blood lipid, it is anti-oxidant, delay senescence, the health-care efficacy of pre- preventing obesity, while reaching
Flavor is complementary, and act synergistically apparent effect, is sufficiently used the bioactive ingredients of the two.
(3) a large amount of research shows that Momordica charantial have it is hypoglycemic, improve immunity of organisms, adjust endocrine, is anti-oxidant
Effect, and propose to Momordica charantial reduce cholesterol, adjust body substance metabolism, potential source biomolecules are active grinds for bacteriostasis etc.
Study carefully and develop, Momordica charantial can promote glucose utilization, play preferable hypoglycemic effect, become hyperglycemic patients reason
The food sources thought；Blue or green money willow extract has the physiological activity such as reducing blood lipid, blood pressure lowering, hypoglycemic, while there are also enhancing human bodies
Physiological functions, the polysaccharide such as immunity, anticancer, prevention and treatment diabetes are one of the important activity ingredients in Qingqian Willow leaf, and blue or green money willow is more
Sugar has the function of significantly improving blood lipid metabolism and improves antioxidant ability of organism.
(4) present inventors have surprisingly found that consumption proportion according to the invention composition containing Momordica charantial and blue or green money willow it is more
The health protection tea of sugar has both lipid-loweringing, pre- preventing obesity, anti-oxidant and anti-aging excellent achievement, and produces apparent collaboration
Effect, health composition of the invention based on natural extract, safety, it is natural, have no toxic side effect, it is easy to use, meet
The health of modern needs, and long-term use has the effect of lipid-loweringing and anti-aging.
Detailed description of the invention
Fig. 1 is the inhibitory activity of Momordica charantial of the present invention and blue or green money willow polyoses extract to pancreatic lipase；
Fig. 2 is the influence of Momordica charantial of the present invention and blue or green money willow polyoses extract to the mobile power of nematode；
Fig. 3 A is the nematode intestines lipofuscin fluorescence that Momordica charantial of the present invention and blue or green money willow polyoses extract are handled；
Fig. 3 B is qualitative analysis intestines lipofuscin relative intensity of fluorescence of the present invention；
Fig. 4 A is the nematode fat oil red O stain that Momordica charantial of the present invention and blue or green money willow polyoses extract are handled；
Fig. 4 B is qualitative analysis fat stains intensity of the present invention；
Fig. 5 A is fat accumulation model foundation；
Fig. 5 B is the opposite fat content of glucose nematode before and after the processing；
Fig. 5 C is the nematode fat oil red O stain high in fat of balsam pear Qingqian willow health tea of the present invention processing；
Fig. 5 D is the nematode high in fat of balsam pear Qingqian willow health tea of the present invention processing with respect to fat content；
Fig. 6 is the nematode ROS relative amount of balsam pear Qingqian willow health tea processing of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1:
Weigh 50g Momordica charantial extract, 2000g blue or green money willow polyoses extract, 200g xylitol, 20g citric acid, 5g sweet tea
Synanthrin is dissolved in 10L water, through homogeneous (20MPa, 2 times) and degassing after allotment uniformly.It is carried out at once after sterilizing 15min using boiling water bath
Hot filling, capping.Then sub-sectional cooling (respectively with 75 DEG C, 50 DEG C, tap water at normal temperature) is used, until close to room temperature.Finally examine
It tests various microbiological indicators and physicochemical property and is stored, oral liquid formulation balsam pear blue or green money willow lipid-lowering health-care tea is made.
Embodiment 2:
Weigh 10g Momordica charantial extract, 1000g blue or green money willow polyoses extract, 225g xylitol, 12g citric acid, 2g sweet tea
Synanthrin is dissolved in 1L water, through homogeneous (20MPa, 2 times) and degassing after allotment uniformly.It is carried out at once after sterilizing 15min using boiling water bath
Hot filling, capping.Then sub-sectional cooling (respectively with 75 DEG C, 50 DEG C, tap water at normal temperature) is used, until close to room temperature.Finally examine
It tests various microbiological indicators and physicochemical property and is stored, oral liquid formulation balsam pear blue or green money willow lipid-lowering health-care tea is made.Optionally
, particle electuary health protection tea is made using the method for spray drying in oral liquid formulation health protection tea obtained, or 4 times of volumes are added
Syrup oral solution health protection tea is made.Optional, particle electuary health protection tea obtained is subjected to pulverization process through pulverizer
Then syrup is directly sprayed onto tea powder by (8000r/min) through atomizing pelletizer, the fine particle formed is packed into capsule
Shell forms capsule or repressed molding machine is tabletted under 5t pressure, and cooling down and wrapping sweet tablet forms tablet.
Embodiment 3:
Momordica charantial and blue or green money willow polyoses extract are to pancreatic lipase inhibitory activity
Using Copoloid-spectrophotometry measurement Momordica charantial and the external suppression enzyme activity of blue or green money willow polyoses extract, pass through
It measures it and Lipid-lowering activities is evaluated to the inhibiting rate of pancreatic lipase activity.As blue or green money willow polysaccharide presses down Fig. 1 at same concentration (10mg/ml)
Rate processed is that 47.09 ± 0.07%, Momordica charantial inhibiting rate is 43.91 ± 0.16%, and orlistat (positive control) inhibiting rate is
69.19 ± 2.56%, blue or green money willow polysaccharide, Momordica charantial extract Inhibiting enzyme activity reach orlistat Inhibiting enzyme activity respectively
68.06%, 63.46%, blue or green money willow polysaccharide, Momordica charantial extract as natural fat enzyme inhibitor have considerable lipid-loweringing
Activity.
Embodiment 4:
Momordica charantial and blue or green money willow polyoses extract and the two compound the influence to nematode movement ability
Four experimental groups, respectively blank control group (CK), Momordica charantial group (K) are set, and blue or green money willow polysaccharide group (Q) is answered
Combo (K+Q).According to the concentration of effective component each in beverage product, Q group feeds nematode with containing 10% blue or green money willow polysaccharide extraction liquid；
K group feeds nematode with containing 0.1% Momordica charantial extracting solution；K+Q group takes 10% blue or green money willow polysaccharide and 0.1% Momordica charantial to extract
The proportion of liquid 5: 5 feeds nematode (symbol is the same below).The motion conditions of nematode are divided into three grades: 1. nematode autogenic movement, no
It needs to touch stimulation, is denoted as I；2. nematode must just be moved by touching stimulation, it is denoted as II；3. nematode is by after touching stimulation
It swings head or tail, is denoted as III (nematode service life number of days was counted from egg hatching first day).
The experimental results showed that Momordica charantial, blue or green money willow polysaccharide can effectively delay the degeneration of caenorhabditis elegan locomotivity, later period
It is especially apparent, wherein the effect after compounding is best.As shown in Figure 2, on day 4, all groups of nematode carries out quickly having rhythm
Autogenic movement, motion conditions are without significant difference.At the 7th day, each group occurred what nematode must just be moved by touching stimulation
Situation, wherein compounding group occurrence rate minimum 3.33%.At the 11st day, the speed of the spontaneous movement of nematode energy was decreased obviously, nematode
Occurred by the case where only swinging head or tail after touching stimulation, relative to control group, the situation of movement of experimental group is obviously improved, especially
It is that nematode unwanted movement the situation II and III of the processing of compounding group are considerably less than control group.
Embodiment 5:
Momordica charantial and blue or green money willow polyoses extract and the two compound the influence to nematode intestines lipofuscin
Marker of the intestines lipofuscin as aging is the acidic oxidation products of cell, and with aging, content can be got over
Come higher.15 nematodes of every group of random selection are placed on glass slide, are clapped by fluorescence microscope using 100 times of amplification factor
It takes the photograph, and carries out the fluorescence intensity of photodensitometry nematode using image J software.Every group is at least repeated 3 times.
Experiment, which shows Momordica charantial, blue or green money willow polysaccharide, can efficiently reduce the intracorporal intestines lipofuscin accumulation of nematode, compounding
Reducing effect is more significant afterwards.Polypide intestines lipofuscin fluorescence after sample treatment obviously weakens (Fig. 3 A), and qualitative analysis fluorescence is strong
Result such as Fig. 3 B is spent, the 3rd, 7,11 day, sample treatment group can reduce significantly intracorporal intestines lipofuscin (the P < of caenorhabditis elegan
0.05), sample is handled relative to balsam pear processing group and blue or green money willow in the intestines lipofuscin content of the 11st day complex treatment group caenorhabditis elegan
Product conspicuousness reduces, and shows that the two has the function of centainly mutually promoting and reduces the generation of intestines lipofuscin.
Embodiment 6:
The influence of Momordica charantial and blue or green money willow polyoses extract and the two compounding to prevention fat deposition
After oil red O stain is handled, polypide has different degrees of coloring phenomenon, staining power and body fat relative amount
It is proportional.7th day nematode oil red O dye liquor is dyed, 15 nematodes of every group of random selection are placed on glass slide, are put
Big 400 times are taken pictures, and analyze staining power with the shooting of monochromatic digital camera and image J software, every group is at least repeated 3 times.
The result shows that Momordica charantial and blue or green money willow polysaccharide can play the role of significantly preventing nematode intestinal fat deposition,
Wherein composite sample better effect.Compared with the control, the polypide dyeing of Momordica charantial and the processing of blue or green money willow polysaccharide extraction liquid is compared
Control group is shallow, and it is more shallow than the polypide dyeing for containing only Momordica charantial or the processing of blue or green money willow polysaccharide sample to compound sample treatment
(Fig. 4 A).Compared with the control group, it can significantly reduce the intracorporal fat content of nematode (P < 0.05) after sample treatment 7 days for three kinds,
And composite sample treatment effect is most significant, and 33.23% (such as Fig. 4 B) of nematode intestinal fat can be reduced relative to control group, is shown
The two has the function of pre- preventing obesity of centainly mutually promoting.
Embodiment 7:
Influence of the balsam pear Qingqian willow health tea to fat deposition is inhibited
Five sample sets are set, are blue or green money willow polysaccharide group (Q), Momordica charantial group (K), compounding group (K+Q) and drink respectively
Material group (Y) (symbol is the same below), wherein beverage processing group uses the drink health-care tea concentrate of 10% vacuum concentration of experimental example 2
(10 times of concentrations) processing nematode.
With 3 days building hyperlipidemia models of glucose induction L1 phase nematode, as a result such as Fig. 5 A, the polypide after glucose induction contaminates
Intensity of colour is apparently higher than control group.Through further quantitative analysis it is found that high in fat group of nematode fat content is significantly higher than control group
(5B), this illustrates the fat accumulation model foundation success of the nematode under the induction of glucose.
To probe into effect of the balsam pear Qingqian willow health tea to nematode fat deposition is inhibited, 50 μM of glucose is handled 3 days
The sample culturing 4 days of nematode high in fat afterwards simultaneously carries out oil red O stain, the experimental results showed that, it can be substantially reduced after sample treatment
Nematode body fat content, wherein compounding group effect is best, is secondly beverage group, is finally Momordica charantial, at blue or green money willow polysaccharide
Reason group (P < 0.05).Sample sets processing after polypide dyeing it is shallower relative to control group, the fat content of K, Q, K+Q, Y group with it is right
It is compared according to group and declines 13.79%, 9.73%, 25.97% respectively, 18.46% (such as Fig. 5 C, D).This shows Momordica charantial, green money
Willow polysaccharide can reduce the intracorporal fat content of nematode, and more preferably, while balsam pear Qingqian willow health tea also has the two compounding effect
Considerable Lipid-lowering activities.
Embodiment 8:
Influence of the balsam pear Qingqian willow health tea to being accumulated to ROS in nematode body
Free-radical theory thinks, the balance that free radical is generated and removed is destroyed with advancing age, it is excessive from
It is the main reason for leading to aging and many age-related diseases by base.Compared with the control group, the intracorporal ROS of sample sets nematode
Level is remarkably decreased (P < 0.05), and wherein the ROS of K group, Q group and K+Q group declines more significant (P < horizontally relative to Y group
0.05) (Fig. 6) illustrates that Momordica charantial, blue or green money willow polysaccharide and its compositional liquor and manufactured beverage product can efficiently reduce show
The accumulation of ROS, delays the aging of nematode in beautiful hidden rhabditida body.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (10)
1. a kind of balsam pear blue or green money willow lipid-lowering health-care tea, which is characterized in that the raw material including following parts by weight:
Momordica charantial extract 0.1~10%, blue or green money willow polyoses extract 5~35%, xylitol 1~10%, citric acid 0.1~
2%, stevioside 0.01~1%.
2. a kind of balsam pear blue or green money willow lipid-lowering health-care tea according to claim 1, which is characterized in that the balsam pear blue or green money willow drop
Rouge health protection tea includes the raw material of following parts by weight:
Momordica charantial extract 0.1%, blue or green money willow polyoses extract 10%, xylitol 2.25%, citric acid 0.12%, stevioside
0.02%.
3. a kind of balsam pear blue or green money willow lipid-lowering health-care tea according to claim 1 or 2, which is characterized in that the balsam pear blueness money
Acceptable auxiliary material is also added in willow lipid-lowering health-care tea.
4. a kind of balsam pear blue or green money willow lipid-lowering health-care tea according to claim 3, which is characterized in that the acceptable auxiliary material
For fructose syrup, potassium sorbate, white granulated sugar, citric acid, malic acid, sodium citrate, calgon, xylitol, stevioside, sugarcane
Sugar, pregelatinized starch, Aspartame, powder-beta-dextrin, agar, pectin, vitamin C, vitamin B, vitamin D, xylitol, hydroxypropyl
One of PASELLI EASYGEL, oligoisomaltose or a variety of compositions.
5. a kind of balsam pear blue or green money willow lipid-lowering health-care tea according to claim 1 or 2, which is characterized in that the health protection tea is
Particle electuary, oral solution, drink, capsule or tablet.
6. a kind of preparation method of balsam pear blue or green money willow lipid-lowering health-care tea according to claim 5, which is characterized in that the hardship
The preparation method of melon blue or green money willow lipid-lowering health-care tea the following steps are included:
Step 1: the extraction of Momordica charantial
Pretreatment of raw material: select appearance it is normal, without rot, the balsam pear without insect pest, half deseeding is cut after cleaning, is sliced, and protected
Color and de- suffering reason dry 12h at 55 DEG C, and moisture content is 4~5%, pulverizes, crosses 60 meshes, be stored in drier to
With；
It extracts: balsam pear powder is added to 70% ethyl alcohol for being preheating to certain temperature by 1: 20 solid-liquid ratio, be 60 DEG C in extraction temperature, extraction
Pressure power be 0.5MPa, resolution temperature be 60 DEG C, extraction time be 60min under the conditions of carry out low temperature continuous phase transistion extraction, through from
The heart, filtering, centrifugal speed 4000r/min, centrifugation time collect filter residue and filtrate respectively after being 5min, filter residue carries out same batten
The second extraction of part, the filtrate extracted twice is merged and is concentrated into the 1/5 of original volume, and drying washing 3 times obtains balsam pear after dry
Saponin extract, recovery rate 12.58%, saponin(e purity are 64.5%, and 4 DEG C save backup；
Step 2: the extraction of blue or green money willow polysaccharide
Pretreatment of raw material: selecting that appearance is normal, disease-free blue or green money willow, removes limb, shreds after being cleaned, dry at 55 DEG C
12h, moisture content are 3~4%, and stand-by after 40 meshes of pulverizing；
It extracts: blue or green money willow powder ultrasonic wave added hot water being extracted into polysaccharide, solid-liquid ratio 1: 10, temperature is 85 DEG C, extracts 3
Secondary, filtrate is concentrated by evaporation to the 1/4 of original volume after suction filtration by each 2h, is placed in 4 DEG C of alcohol precipitations and stays overnight, is in centrifugal speed
It abandons supernatant after 3500r/min, centrifugation 10min to carry out being freeze-dried to obtain crude extract, and by crude extract with 80% ethanol washing three times
After be freeze-dried up to blue or green money willow polyoses extract, recovery rate 10.25%, purity 78.2%, molecular weight 900kDa
~1160kDa, 4 DEG C save backup；
Step 3: balsam pear-Qingqian willow health tea development process
The extract and xylitol, citric acid, stevioside auxiliary material of step 1 and step 2 are dissolved in water allotment uniformly in proportion,
15min is sterilized using boiling water bath after homogeneous and degassing, carries out hot filling at once, is covered, sub-sectional cooling is then used, until close
Room temperature is finally examined various microbiological indicators and physicochemical property and is stored, and oral liquid formulation health protection tea is made；
Step 4: particle electuary health care is made using the method for spray drying in oral liquid formulation health protection tea made from step 3
Health tea is made in tea, or the syrup of 4 times of volumes of addition；
Step 5: particle electuary health protection tea made from step 4 is subjected to pulverization process through pulverizer, pulverizer revolving speed is
8000r/min, then syrup is directly sprayed onto tea powder through atomizing pelletizer, the fine particle formed is packed into capsule shell shape
Tabletted under 5t pressure at capsule or repressed molding machine, cooling down and wrapping sweet tablet forms tablet.
7. a kind of preparation method of balsam pear blue or green money willow lipid-lowering health-care tea according to claim 6, which is characterized in that the step
In rapid three processing condition be homogeneous 2 times under the conditions of high pressure homogenizer 20MPa, sub-sectional cooling condition be respectively with 75 DEG C, 50 DEG C,
Tap water at normal temperature sub-sectional cooling.
8. a kind of preparation method of balsam pear blue or green money willow lipid-lowering health-care tea according to claim 6, which is characterized in that the hardship
Melon saponin(e and blue or green money willow polysaccharide active components can be used conventional extracting method and extract.
9. a kind of if the described in any item balsam pear blue or green money willow lipid-lowering health-care teas of claim 1-8 are in lipid-loweringing, pre- preventing obesity, antioxygen
Application in changing, delaying senescence.
10. balsam pear blue or green money willow lipid-lowering health-care tea according to claim 9, which is characterized in that its described lipid-loweringing, prevention fertilizer
Appraisement system that is fat, anti-oxidant and delaying senescence includes Caenorhabditis elegans model.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631288A (en) * | 2020-05-29 | 2020-09-08 | 重庆医药高等专科学校 | Bitter gourd formula particle health tea and preparation method thereof |
| CN112120204A (en) * | 2020-10-27 | 2020-12-25 | 湖南华诚生物资源股份有限公司 | Sweet composition for reducing blood fat and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102525945A (en) * | 2012-01-11 | 2012-07-04 | 南京林业大学 | Preparation method for cyclocarya paliurus(Batal)Ijinskaja hypoglycemic granules |
| CN105265690A (en) * | 2015-11-13 | 2016-01-27 | 瞿亮亮 | Cyclocarya paliurus bitter gourd tea and making method thereof |
| CN106692297A (en) * | 2017-02-15 | 2017-05-24 | 云南德彩堂生物医药科技有限公司 | Composition for assistedly decreasing blood sugar, and preparation method and application thereof |
| CN107136269A (en) * | 2017-06-29 | 2017-09-08 | 北京颐方生物科技有限公司 | A kind of blue or green money willow bitter gourd sugar-reducing tea and preparation method thereof |
| CN107647054A (en) * | 2017-10-26 | 2018-02-02 | 霞浦县丰乔农业科技发展有限公司 | A kind of blue or green money willow tea powder and preparation method thereof |
| CN108077727A (en) * | 2017-12-18 | 2018-05-29 | 中科国思生物科技研究（广州）有限公司 | A kind of lotus leaf balsam pear plant amylum food of reducing blood lipid and preparation method thereof |
-
2018
- 2018-06-28 CN CN201810695020.3A patent/CN108935842A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102525945A (en) * | 2012-01-11 | 2012-07-04 | 南京林业大学 | Preparation method for cyclocarya paliurus(Batal)Ijinskaja hypoglycemic granules |
| CN105265690A (en) * | 2015-11-13 | 2016-01-27 | 瞿亮亮 | Cyclocarya paliurus bitter gourd tea and making method thereof |
| CN106692297A (en) * | 2017-02-15 | 2017-05-24 | 云南德彩堂生物医药科技有限公司 | Composition for assistedly decreasing blood sugar, and preparation method and application thereof |
| CN107136269A (en) * | 2017-06-29 | 2017-09-08 | 北京颐方生物科技有限公司 | A kind of blue or green money willow bitter gourd sugar-reducing tea and preparation method thereof |
| CN107647054A (en) * | 2017-10-26 | 2018-02-02 | 霞浦县丰乔农业科技发展有限公司 | A kind of blue or green money willow tea powder and preparation method thereof |
| CN108077727A (en) * | 2017-12-18 | 2018-05-29 | 中科国思生物科技研究（广州）有限公司 | A kind of lotus leaf balsam pear plant amylum food of reducing blood lipid and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631288A (en) * | 2020-05-29 | 2020-09-08 | 重庆医药高等专科学校 | Bitter gourd formula particle health tea and preparation method thereof |
| CN112120204A (en) * | 2020-10-27 | 2020-12-25 | 湖南华诚生物资源股份有限公司 | Sweet composition for reducing blood fat and preparation method thereof |
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Application publication date: 20181207 |